Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23049.t1 | GAU47991.1 | 72.9 | 229 | 53 | 1 | 1 | 229 | 129 | 348 | 6.70E-89 | 337 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23049.t1 | Q84WU2 | 40.3 | 72 | 42 | 1 | 4 | 75 | 112 | 182 | 3.8e-08 | 60.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23049.t1 | A0A2Z6NUX7 | 72.9 | 229 | 53 | 1 | 1 | 229 | 129 | 348 | 4.8e-89 | 337.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23049.t1 | MTR_7g107160 | 77.391 | 230 | 22 | 3 | 1 | 229 | 193 | 393 | 4.17e-109 | 321 |
| MS.gene23049.t1 | MTR_7g107160 | 49.550 | 111 | 55 | 1 | 1 | 111 | 1 | 110 | 6.54e-27 | 107 |
| MS.gene23049.t1 | MTR_7g107200 | 47.059 | 255 | 105 | 3 | 3 | 228 | 72 | 325 | 3.69e-69 | 217 |
| MS.gene23049.t1 | MTR_1g042160 | 42.358 | 229 | 95 | 5 | 1 | 229 | 69 | 260 | 7.69e-50 | 165 |
| MS.gene23049.t1 | MTR_8g011730 | 37.154 | 253 | 126 | 6 | 2 | 229 | 72 | 316 | 1.55e-48 | 163 |
| MS.gene23049.t1 | MTR_7g107170 | 59.091 | 110 | 37 | 1 | 120 | 229 | 114 | 215 | 3.08e-36 | 129 |
| MS.gene23049.t1 | MTR_7g107170 | 81.250 | 48 | 9 | 0 | 182 | 229 | 1 | 48 | 2.16e-20 | 87.0 |
| MS.gene23049.t1 | MTR_2g034780 | 48.544 | 103 | 50 | 1 | 127 | 229 | 119 | 218 | 1.23e-25 | 101 |
| MS.gene23049.t1 | MTR_5g091450 | 37.500 | 72 | 44 | 1 | 1 | 72 | 88 | 158 | 3.00e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23049.t1 | AT5G06600 | 38.667 | 75 | 45 | 1 | 1 | 75 | 110 | 183 | 1.26e-11 | 64.3 |
| MS.gene23049.t1 | AT5G06600 | 38.667 | 75 | 45 | 1 | 1 | 75 | 110 | 183 | 1.27e-11 | 64.3 |
| MS.gene23049.t1 | AT5G06600 | 38.667 | 75 | 45 | 1 | 1 | 75 | 109 | 182 | 1.27e-11 | 64.3 |
| MS.gene23049.t1 | AT3G11910 | 38.667 | 75 | 45 | 1 | 1 | 75 | 109 | 182 | 4.77e-11 | 62.4 |
| MS.gene23049.t1 | AT3G11910 | 38.667 | 75 | 45 | 1 | 1 | 75 | 109 | 182 | 4.77e-11 | 62.4 |
| MS.gene23049.t1 | AT3G11910 | 38.667 | 75 | 45 | 1 | 1 | 75 | 108 | 181 | 5.05e-11 | 62.4 |
| MS.gene23049.t1 | AT3G11910 | 38.667 | 75 | 45 | 1 | 1 | 75 | 108 | 181 | 5.05e-11 | 62.4 |
Find 43 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAAGAAGTTGTAAGTTTA+GGG | 0.193564 | 7.2:+7131447 | MS.gene23049:CDS |
| AGAAAAGAAGTTGTAAGTTT+AGG | 0.206519 | 7.2:+7131446 | MS.gene23049:CDS |
| TGGCTGGGGTATTGGATCTT+TGG | 0.242019 | 7.2:+7131105 | MS.gene23049:CDS |
| GGAATGGGCGTTCACTGCTT+TGG | 0.299493 | 7.2:+7131468 | MS.gene23049:CDS |
| CTTATGGATTTCAAGGGTTT+AGG | 0.322047 | 7.2:+7131349 | MS.gene23049:CDS |
| GCAAAATCCAGGTGAACTTA+TGG | 0.322911 | 7.2:+7131333 | MS.gene23049:CDS |
| GTAAAAGATGTTTGTATTGT+TGG | 0.326070 | 7.2:+7131169 | MS.gene23049:CDS |
| AGCTCCAATGGCTGGGGTAT+TGG | 0.327929 | 7.2:+7131097 | MS.gene23049:CDS |
| GAATGGGCGTTCACTGCTTT+GGG | 0.331310 | 7.2:+7131469 | MS.gene23049:CDS |
| TAGAGGTCAAACTGAAAATC+TGG | 0.352353 | 7.2:+7131270 | MS.gene23049:CDS |
| TGGGAGGAACTTCAGCCATT+TGG | 0.359556 | 7.2:+7131568 | MS.gene23049:CDS |
| TCACTGCAAGCTCCAATGGC+TGG | 0.398816 | 7.2:+7131089 | MS.gene23049:CDS |
| CACTGCAAGCTCCAATGGCT+GGG | 0.419346 | 7.2:+7131090 | MS.gene23049:CDS |
| TATGAATGACTTATCTTGTA+AGG | 0.428376 | 7.2:+7131531 | MS.gene23049:CDS |
| CCAGGAACTTCTTCTAGCAT+TGG | 0.429065 | 7.2:-7131310 | None:intergenic |
| AGGTGAACTTATGGATTTCA+AGG | 0.444860 | 7.2:+7131342 | MS.gene23049:CDS |
| GTTCTTCATGATCCTCATAG+TGG | 0.455915 | 7.2:+7131139 | MS.gene23049:CDS |
| CCAATGCTAGAAGAAGTTCC+TGG | 0.456723 | 7.2:+7131310 | MS.gene23049:CDS |
| ATCTGGAAGCTGAAGTCATA+AGG | 0.473546 | 7.2:+7131287 | MS.gene23049:CDS |
| AAGTTGTAAGTTTAGGGAAT+GGG | 0.480762 | 7.2:+7131453 | MS.gene23049:CDS |
| GAAGTTGTAAGTTTAGGGAA+TGG | 0.491256 | 7.2:+7131452 | MS.gene23049:CDS |
| AGTTTGACCTCTAGATGTAA+AGG | 0.494100 | 7.2:-7131260 | None:intergenic |
| GAAGTTCCTGGGCAAAATCC+AGG | 0.501134 | 7.2:+7131322 | MS.gene23049:CDS |
| TTCTTCATGATCCTCATAGT+GGG | 0.502613 | 7.2:+7131140 | MS.gene23049:CDS |
| TGCTGGGACTCAACAAGTGA+AGG | 0.505102 | 7.2:-7131424 | None:intergenic |
| GAACACTCTTCTACTAGTAA+TGG | 0.512065 | 7.2:-7131394 | None:intergenic |
| AGATCCAATACCCCAGCCAT+TGG | 0.519689 | 7.2:-7131101 | None:intergenic |
| GAGTTCACTGCAAGCTCCAA+TGG | 0.520868 | 7.2:+7131085 | MS.gene23049:CDS |
| TTTACAAGAAACCCACTATG+AGG | 0.523336 | 7.2:-7131151 | None:intergenic |
| CAAACTGCTAACATGTCTAA+AGG | 0.546098 | 7.2:+7130818 | None:intergenic |
| ACTACTTCCTTTACATCTAG+AGG | 0.550454 | 7.2:+7131253 | MS.gene23049:CDS |
| CTGCTAACATGTCTAAAGGA+TGG | 0.552141 | 7.2:+7130822 | None:intergenic |
| TTGAAATCCATAAGTTCACC+TGG | 0.559661 | 7.2:-7131340 | None:intergenic |
| TGTTTGTATTGTTGGTGTAG+AGG | 0.561083 | 7.2:+7131177 | MS.gene23049:CDS |
| GTATCATGTTCAATCAGCCC+TGG | 0.564297 | 7.2:+7131612 | MS.gene23049:CDS |
| TTGTTCTGTTGCTGTCGACC+TGG | 0.565780 | 7.2:-7130880 | None:intergenic |
| TAAGTTGTTTGTTCTTAACC+AGG | 0.569810 | 7.2:+7130862 | MS.gene23049:CDS |
| GGTGAACTTATGGATTTCAA+GGG | 0.574063 | 7.2:+7131343 | MS.gene23049:CDS |
| AACAGAACAATCTTCTCTGA+TGG | 0.582000 | 7.2:+7130893 | MS.gene23049:CDS |
| CAATGCTAGAAGAAGTTCCT+GGG | 0.601828 | 7.2:+7131311 | MS.gene23049:CDS |
| ACTGCAAGCTCCAATGGCTG+GGG | 0.608500 | 7.2:+7131091 | MS.gene23049:CDS |
| TATCATGTTCAATCAGCCCT+GGG | 0.620754 | 7.2:+7131613 | MS.gene23049:CDS |
| GCTGGGACTCAACAAGTGAA+GGG | 0.631377 | 7.2:-7131423 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTATTTTTCTTATGG+TGG | + | chr7.2:7130920-7130939 | MS.gene23049:intron | 15.0% |
| !!! | TAAGGATCTTCAAATTTTTT+GGG | + | chr7.2:7131549-7131568 | MS.gene23049:CDS | 20.0% |
| ! | AGAAAAGAAGTTGTAAGTTT+AGG | + | chr7.2:7131446-7131465 | MS.gene23049:CDS | 25.0% |
| ! | CAAGACAAATAAAAACCAAA+TGG | - | chr7.2:7131586-7131605 | None:intergenic | 25.0% |
| ! | GAAAAGAAGTTGTAAGTTTA+GGG | + | chr7.2:7131447-7131466 | MS.gene23049:CDS | 25.0% |
| ! | GTAAAAGATGTTTGTATTGT+TGG | + | chr7.2:7131169-7131188 | MS.gene23049:CDS | 25.0% |
| ! | TATGAATGACTTATCTTGTA+AGG | + | chr7.2:7131531-7131550 | MS.gene23049:CDS | 25.0% |
| !!! | AAGTTTTTTGGAGAAAATGA+AGG | + | chr7.2:7131642-7131661 | MS.gene23049:CDS | 25.0% |
| !!! | CATTTGGTTTTTATTTGTCT+TGG | + | chr7.2:7131584-7131603 | MS.gene23049:CDS | 25.0% |
| !!! | GTAAGGATCTTCAAATTTTT+TGG | + | chr7.2:7131548-7131567 | MS.gene23049:CDS | 25.0% |
| AAGTTGTAAGTTTAGGGAAT+GGG | + | chr7.2:7131453-7131472 | MS.gene23049:CDS | 30.0% | |
| GAACAACACGTAAAAAGAAA+GGG | - | chr7.2:7130958-7130977 | None:intergenic | 30.0% | |
| TAAGTTGTTTGTTCTTAACC+AGG | + | chr7.2:7130862-7130881 | MS.gene23049:CDS | 30.0% | |
| TGAACAACACGTAAAAAGAA+AGG | - | chr7.2:7130959-7130978 | None:intergenic | 30.0% | |
| ! | CAAAAAACTTTTCATTCCCA+GGG | - | chr7.2:7131632-7131651 | None:intergenic | 30.0% |
| !!! | CTTTCACTTTTCTAGTCTTA+AGG | - | chr7.2:7131510-7131529 | None:intergenic | 30.0% |
| !!! | TTTTTTGGAGAAAATGAAGG+AGG | + | chr7.2:7131645-7131664 | MS.gene23049:CDS | 30.0% |
| AACAGAACAATCTTCTCTGA+TGG | + | chr7.2:7130893-7130912 | MS.gene23049:CDS | 35.0% | |
| ACTACTTCCTTTACATCTAG+AGG | + | chr7.2:7131253-7131272 | MS.gene23049:CDS | 35.0% | |
| AGGTGAACTTATGGATTTCA+AGG | + | chr7.2:7131342-7131361 | MS.gene23049:CDS | 35.0% | |
| AGTTTGACCTCTAGATGTAA+AGG | - | chr7.2:7131263-7131282 | None:intergenic | 35.0% | |
| CTTATGGATTTCAAGGGTTT+AGG | + | chr7.2:7131349-7131368 | MS.gene23049:CDS | 35.0% | |
| GAACACTCTTCTACTAGTAA+TGG | - | chr7.2:7131397-7131416 | None:intergenic | 35.0% | |
| GAAGTTGTAAGTTTAGGGAA+TGG | + | chr7.2:7131452-7131471 | MS.gene23049:CDS | 35.0% | |
| GGTGAACTTATGGATTTCAA+GGG | + | chr7.2:7131343-7131362 | MS.gene23049:CDS | 35.0% | |
| TAGAGGTCAAACTGAAAATC+TGG | + | chr7.2:7131270-7131289 | MS.gene23049:CDS | 35.0% | |
| TGTTTGTATTGTTGGTGTAG+AGG | + | chr7.2:7131177-7131196 | MS.gene23049:CDS | 35.0% | |
| TTCTTCATGATCCTCATAGT+GGG | + | chr7.2:7131140-7131159 | MS.gene23049:CDS | 35.0% | |
| TTGAAATCCATAAGTTCACC+TGG | - | chr7.2:7131343-7131362 | None:intergenic | 35.0% | |
| TTTACAAGAAACCCACTATG+AGG | - | chr7.2:7131154-7131173 | None:intergenic | 35.0% | |
| ! | ACTTACAACTTCTTTTCTGC+TGG | - | chr7.2:7131444-7131463 | None:intergenic | 35.0% |
| ! | CCAAAAAACTTTTCATTCCC+AGG | - | chr7.2:7131633-7131652 | None:intergenic | 35.0% |
| ! | CTTACAACTTCTTTTCTGCT+GGG | - | chr7.2:7131443-7131462 | None:intergenic | 35.0% |
| !!! | CCTGGGAATGAAAAGTTTTT+TGG | + | chr7.2:7131630-7131649 | MS.gene23049:CDS | 35.0% |
| !!! | GGATCTTCAAATTTTTTGGG+AGG | + | chr7.2:7131552-7131571 | MS.gene23049:CDS | 35.0% |
| !!! | TGATTCATGTTTTTTCCCAG+AGG | + | chr7.2:7131054-7131073 | MS.gene23049:intron | 35.0% |
| ATCTGGAAGCTGAAGTCATA+AGG | + | chr7.2:7131287-7131306 | MS.gene23049:CDS | 40.0% | |
| CAATGCTAGAAGAAGTTCCT+GGG | + | chr7.2:7131311-7131330 | MS.gene23049:CDS | 40.0% | |
| GCAAAATCCAGGTGAACTTA+TGG | + | chr7.2:7131333-7131352 | MS.gene23049:CDS | 40.0% | |
| GTTCTTCATGATCCTCATAG+TGG | + | chr7.2:7131139-7131158 | MS.gene23049:CDS | 40.0% | |
| TATCATGTTCAATCAGCCCT+GGG | + | chr7.2:7131613-7131632 | MS.gene23049:CDS | 40.0% | |
| CCAATGCTAGAAGAAGTTCC+TGG | + | chr7.2:7131310-7131329 | MS.gene23049:CDS | 45.0% | |
| CCAGGAACTTCTTCTAGCAT+TGG | - | chr7.2:7131313-7131332 | None:intergenic | 45.0% | |
| GTATCATGTTCAATCAGCCC+TGG | + | chr7.2:7131612-7131631 | MS.gene23049:CDS | 45.0% | |
| !!! | ATTTTTTTTTATTTTTCTTA+TGG | + | chr7.2:7130917-7130936 | MS.gene23049:intron | 5.0% |
| AGATCCAATACCCCAGCCAT+TGG | - | chr7.2:7131104-7131123 | None:intergenic | 50.0% | |
| GAAGTTCCTGGGCAAAATCC+AGG | + | chr7.2:7131322-7131341 | MS.gene23049:CDS | 50.0% | |
| GAATGGGCGTTCACTGCTTT+GGG | + | chr7.2:7131469-7131488 | MS.gene23049:CDS | 50.0% | |
| GAGTTCACTGCAAGCTCCAA+TGG | + | chr7.2:7131085-7131104 | MS.gene23049:CDS | 50.0% | |
| GCTGGGACTCAACAAGTGAA+GGG | - | chr7.2:7131426-7131445 | None:intergenic | 50.0% | |
| TGAACTCGTGTTGAGCCTCT+GGG | - | chr7.2:7131072-7131091 | None:intergenic | 50.0% | |
| TGCTGGGACTCAACAAGTGA+AGG | - | chr7.2:7131427-7131446 | None:intergenic | 50.0% | |
| TGGGAGGAACTTCAGCCATT+TGG | + | chr7.2:7131568-7131587 | MS.gene23049:CDS | 50.0% | |
| TTGTTCTGTTGCTGTCGACC+TGG | - | chr7.2:7130883-7130902 | None:intergenic | 50.0% | |
| ! | AGTTCACCTGGATTTTGCCC+AGG | - | chr7.2:7131331-7131350 | None:intergenic | 50.0% |
| !! | TGGCTGGGGTATTGGATCTT+TGG | + | chr7.2:7131105-7131124 | MS.gene23049:CDS | 50.0% |
| ACTGCAAGCTCCAATGGCTG+GGG | + | chr7.2:7131091-7131110 | MS.gene23049:CDS | 55.0% | |
| AGCTCCAATGGCTGGGGTAT+TGG | + | chr7.2:7131097-7131116 | MS.gene23049:CDS | 55.0% | |
| CACTGCAAGCTCCAATGGCT+GGG | + | chr7.2:7131090-7131109 | MS.gene23049:CDS | 55.0% | |
| GGAATGGGCGTTCACTGCTT+TGG | + | chr7.2:7131468-7131487 | MS.gene23049:CDS | 55.0% | |
| GTGAACTCGTGTTGAGCCTC+TGG | - | chr7.2:7131073-7131092 | None:intergenic | 55.0% | |
| TCACTGCAAGCTCCAATGGC+TGG | + | chr7.2:7131089-7131108 | MS.gene23049:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 7130830 | 7131678 | 7130830 | ID=MS.gene23049 |
| chr7.2 | mRNA | 7130830 | 7131678 | 7130830 | ID=MS.gene23049.t1;Parent=MS.gene23049 |
| chr7.2 | exon | 7130830 | 7130914 | 7130830 | ID=MS.gene23049.t1.exon1;Parent=MS.gene23049.t1 |
| chr7.2 | CDS | 7130830 | 7130914 | 7130830 | ID=cds.MS.gene23049.t1;Parent=MS.gene23049.t1 |
| chr7.2 | exon | 7131074 | 7131678 | 7131074 | ID=MS.gene23049.t1.exon2;Parent=MS.gene23049.t1 |
| chr7.2 | CDS | 7131074 | 7131678 | 7131074 | ID=cds.MS.gene23049.t1;Parent=MS.gene23049.t1 |
| Gene Sequence |
| Protein sequence |
