AlfalfaGEDB | MS.gene23889

M. sativa cultivar XinJiangDaYe / MS.gene23889

KEGG: K05752
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23889.t1	XP_003517200.1	100	85	0	0	1	85	1	85	2.20E-37	164.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23889.t1	Q94JY4	90.6	85	8	0	1	85	1	85	1.6e-36	152.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23889.t1	A0A0B2PBB9	100.0	85	0	0	1	85	1	85	1.6e-37	164.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene23889	0.821784	3.36E-53	-1.69E-46
MS.gene049341	MS.gene23889	0.814728	1.33E-51	-1.69E-46
MS.gene049821	MS.gene23889	0.80142	9.08E-49	-1.69E-46
MS.gene049850	MS.gene23889	0.823096	1.66E-53	-1.69E-46
MS.gene050020	MS.gene23889	0.848101	7.59E-60	-1.69E-46
MS.gene050303	MS.gene23889	0.804792	1.82E-49	-1.69E-46
MS.gene050355	MS.gene23889	0.802316	5.94E-49	-1.69E-46
MS.gene050442	MS.gene23889	0.823161	1.61E-53	-1.69E-46
MS.gene050807	MS.gene23889	0.813339	2.69E-51	-1.69E-46
MS.gene050815	MS.gene23889	0.847194	1.35E-59	-1.69E-46
MS.gene050844	MS.gene23889	0.803151	4.00E-49	-1.69E-46
MS.gene051247	MS.gene23889	0.815622	8.41E-52	-1.69E-46
MS.gene051328	MS.gene23889	0.809693	1.67E-50	-1.69E-46
MS.gene05157	MS.gene23889	0.821956	3.06E-53	-1.69E-46
MS.gene051719	MS.gene23889	0.823936	1.06E-53	-1.69E-46
MS.gene051720	MS.gene23889	0.811665	6.25E-51	-1.69E-46
MS.gene051744	MS.gene23889	0.809051	2.29E-50	-1.69E-46
MS.gene052012	MS.gene23889	0.805221	1.48E-49	-1.69E-46
MS.gene052020	MS.gene23889	0.808645	2.80E-50	-1.69E-46
MS.gene052519	MS.gene23889	0.832607	8.59E-56	-1.69E-46
MS.gene052584	MS.gene23889	0.801323	9.51E-49	-1.69E-46
MS.gene052611	MS.gene23889	0.85205	5.95E-61	-1.69E-46
MS.gene052612	MS.gene23889	0.806562	7.75E-50	-1.69E-46
MS.gene052863	MS.gene23889	0.824178	9.29E-54	-1.69E-46
MS.gene052896	MS.gene23889	0.800992	1.11E-48	-1.69E-46
MS.gene053008	MS.gene23889	0.82158	3.74E-53	-1.69E-46
MS.gene053226	MS.gene23889	0.840317	9.35E-58	-1.69E-46
MS.gene054236	MS.gene23889	0.806452	8.17E-50	-1.69E-46
MS.gene05462	MS.gene23889	0.80174	7.81E-49	-1.69E-46
MS.gene054775	MS.gene23889	0.810583	1.07E-50	-1.69E-46
MS.gene055166	MS.gene23889	0.810085	1.37E-50	-1.69E-46
MS.gene055357	MS.gene23889	0.80382	2.90E-49	-1.69E-46
MS.gene055358	MS.gene23889	0.80547	1.31E-49	-1.69E-46
MS.gene055465	MS.gene23889	0.81384	2.09E-51	-1.69E-46
MS.gene055466	MS.gene23889	0.849485	3.14E-60	-1.69E-46
MS.gene055468	MS.gene23889	0.819315	1.24E-52	-1.69E-46
MS.gene055778	MS.gene23889	0.828798	7.36E-55	-1.69E-46
MS.gene055818	MS.gene23889	0.847054	1.47E-59	-1.69E-46
MS.gene05604	MS.gene23889	0.806832	6.79E-50	-1.69E-46
MS.gene056162	MS.gene23889	0.80786	4.11E-50	-1.69E-46
MS.gene056215	MS.gene23889	0.823916	1.07E-53	-1.69E-46
MS.gene056498	MS.gene23889	0.804889	1.74E-49	-1.69E-46
MS.gene056595	MS.gene23889	0.817409	3.35E-52	-1.69E-46
MS.gene057060	MS.gene23889	0.839057	1.99E-57	-1.69E-46
MS.gene057314	MS.gene23889	0.800043	1.73E-48	-1.69E-46
MS.gene057693	MS.gene23889	0.846611	1.95E-59	-1.69E-46
MS.gene057915	MS.gene23889	0.813998	1.93E-51	-1.69E-46
MS.gene058084	MS.gene23889	0.806347	8.60E-50	-1.69E-46
MS.gene058168	MS.gene23889	0.802987	4.32E-49	-1.69E-46
MS.gene059151	MS.gene23889	0.830002	3.75E-55	-1.69E-46
MS.gene05954	MS.gene23889	0.817985	2.48E-52	-1.69E-46
MS.gene059842	MS.gene23889	0.810283	1.25E-50	-1.69E-46
MS.gene060322	MS.gene23889	0.819798	9.61E-53	-1.69E-46
MS.gene060429	MS.gene23889	0.83938	1.64E-57	-1.69E-46
MS.gene060553	MS.gene23889	0.811428	7.04E-51	-1.69E-46
MS.gene060572	MS.gene23889	0.812996	3.20E-51	-1.69E-46
MS.gene060803	MS.gene23889	0.844345	8.01E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23889.t1	MTR_5g030935	100.000	85	0	0	1	85	1	85	2.67e-57	171

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23889.t1	AT2G22640	90.588	85	8	0	1	85	1	85	6.83e-53	159

Find 12 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGAAATTCAGTACCGAAT+TGG	0.368507	5.2:+20945472	None:intergenic
TGATGTATTGGAGCGTCGTT+TGG	0.385920	5.2:-20945315	MS.gene23889:CDS
GAATCGCTGTTCAAGCTGAT+TGG	0.386107	5.2:-20945557	MS.gene23889:CDS
TTCGATTTCCTTGTCCAATT+CGG	0.396293	5.2:-20945486	MS.gene23889:intron
GAATGAAAAGCTTGATGTAT+TGG	0.415813	5.2:-20945327	MS.gene23889:CDS
AATCGCTGTTCAAGCTGATT+GGG	0.456401	5.2:-20945556	MS.gene23889:CDS
AGTTCAAGTTGGAAATGCTT+CGG	0.460394	5.2:-20945282	MS.gene23889:CDS
GAATTACTTGAAGTTCAAGT+TGG	0.488446	5.2:-20945293	MS.gene23889:CDS
ATTCCATCAATGGCACGTGC+TGG	0.545650	5.2:-20945606	None:intergenic
CCATCAATGGCACGTGCTGG+TGG	0.566134	5.2:-20945603	None:intergenic
ATTCAGTACCGAATTGGACA+AGG	0.614288	5.2:+20945478	None:intergenic
ATCACAAACGCAGTTAACGT+TGG	0.620945	5.2:-20945579	MS.gene23889:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATAAGATTTCAAATAACA+AGG	+	chr5.2:20945437-20945456	None:intergenic	15.0%
!!!	AATCTTATATCTTTTTTTGA+TGG	-	chr5.2:20945447-20945466	MS.gene23889:intron	15.0%
!!!	ATCTTATATCTTTTTTTGAT+GGG	-	chr5.2:20945448-20945467	MS.gene23889:intron	15.0%
!!!	TGTTATTTTTGTTATTTCAG+AGG	-	chr5.2:20945475-20945494	MS.gene23889:intron	20.0%
!	ATTTCAAATAACAAGGCAAA+AGG	+	chr5.2:20945430-20945449	None:intergenic	25.0%
!	TTTCAAATAACAAGGCAAAA+GGG	+	chr5.2:20945429-20945448	None:intergenic	25.0%
	GAATGAAAAGCTTGATGTAT+TGG	-	chr5.2:20945526-20945545	MS.gene23889:CDS	30.0%
	GAATTACTTGAAGTTCAAGT+TGG	-	chr5.2:20945560-20945579	MS.gene23889:CDS	30.0%
	TTCGATTTCCTTGTCCAATT+CGG	-	chr5.2:20945367-20945386	MS.gene23889:intron	35.0%
!	GAAGAAATTCAGTACCGAAT+TGG	+	chr5.2:20945384-20945403	None:intergenic	35.0%
!!	AGTTCAAGTTGGAAATGCTT+CGG	-	chr5.2:20945571-20945590	MS.gene23889:CDS	35.0%
	AATCGCTGTTCAAGCTGATT+GGG	-	chr5.2:20945297-20945316	MS.gene23889:CDS	40.0%
	ATCACAAACGCAGTTAACGT+TGG	-	chr5.2:20945274-20945293	MS.gene23889:CDS	40.0%
!	ATTCAGTACCGAATTGGACA+AGG	+	chr5.2:20945378-20945397	None:intergenic	40.0%
	GAATCGCTGTTCAAGCTGAT+TGG	-	chr5.2:20945296-20945315	MS.gene23889:CDS	45.0%
!!	TGATGTATTGGAGCGTCGTT+TGG	-	chr5.2:20945538-20945557	MS.gene23889:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	20945256	20945619	20945256	ID=MS.gene23889
chr5.2	mRNA	20945256	20945619	20945256	ID=MS.gene23889.t1;Parent=MS.gene23889
chr5.2	exon	20945487	20945619	20945487	ID=MS.gene23889.t1.exon1;Parent=MS.gene23889.t1
chr5.2	CDS	20945487	20945619	20945487	ID=cds.MS.gene23889.t1;Parent=MS.gene23889.t1
chr5.2	exon	20945256	20945380	20945256	ID=MS.gene23889.t1.exon2;Parent=MS.gene23889.t1
chr5.2	CDS	20945256	20945380	20945256	ID=cds.MS.gene23889.t1;Parent=MS.gene23889.t1

Gene Sequence	>MS.gene23889 ATGGCACGTGCTGGTGGAATCACAAACGCAGTTAACGTTGGAATCGCTGTTCAAGCTGATTGGGAAAATCGTGAATTCATCTCTCACATTTCTCTCAATGTTCGTCGTCTCTTCGATTTCCTTGTCCAATTCGGTACTGAATTTCTTCTCAATTTTCTGTTTTTCTTTGACCCTTTTGCCTTGTTATTTGAAATCTTATATCTTTTTTTGATGGGTTGTTGTTATTTTTGTTATTTCAGAGGCTACAACAAAGAGTAAATTAGCTTCATTGAATGAAAAGCTTGATGTATTGGAGCGTCGTTTGGAATTACTTGAAGTTCAAGTTGGAAATGCTTCGGCAAATCCTTCTCTTTTTGCAACTTGA
Protein sequence	>MS.gene23889.t1 MARAGGITNAVNVGIAVQADWENREFISHISLNVRRLFDFLVQFEATTKSKLASLNEKLDVLERRLELLEVQVGNASANPSLFAT