Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24285.t1 | XP_024638160.1 | 63.9 | 119 | 31 | 3 | 1 | 117 | 1 | 109 | 1.10E-18 | 102.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24285.t1 | I3SX70 | 63.9 | 119 | 31 | 3 | 1 | 117 | 1 | 109 | 7.8e-19 | 102.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene24285 | 0.844259 | 8.44E-59 | -1.69E-46 |
| MS.gene049983 | MS.gene24285 | 0.826887 | 2.12E-54 | -1.69E-46 |
| MS.gene04998 | MS.gene24285 | 0.835873 | 1.30E-56 | -1.69E-46 |
| MS.gene051676 | MS.gene24285 | 0.824187 | 9.25E-54 | -1.69E-46 |
| MS.gene051975 | MS.gene24285 | 0.82265 | 2.11E-53 | -1.69E-46 |
| MS.gene052434 | MS.gene24285 | 0.820736 | 5.86E-53 | -1.69E-46 |
| MS.gene052533 | MS.gene24285 | 0.80553 | 1.28E-49 | -1.69E-46 |
| MS.gene052818 | MS.gene24285 | -0.817625 | 2.99E-52 | -1.69E-46 |
| MS.gene053295 | MS.gene24285 | 0.801307 | 9.58E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene24285 | 0.992562 | 4.81E-194 | -1.69E-46 |
| MS.gene053340 | MS.gene24285 | 0.824993 | 5.97E-54 | -1.69E-46 |
| MS.gene053786 | MS.gene24285 | 0.809039 | 2.30E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene24285 | 0.877443 | 6.26E-69 | -1.69E-46 |
| MS.gene054222 | MS.gene24285 | 0.848227 | 7.01E-60 | -1.69E-46 |
| MS.gene054479 | MS.gene24285 | 0.812143 | 4.92E-51 | -1.69E-46 |
| MS.gene05469 | MS.gene24285 | 0.817577 | 3.07E-52 | -1.69E-46 |
| MS.gene054892 | MS.gene24285 | 0.8318 | 1.36E-55 | -1.69E-46 |
| MS.gene054893 | MS.gene24285 | 0.83326 | 5.91E-56 | -1.69E-46 |
| MS.gene05517 | MS.gene24285 | 0.802349 | 5.85E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene24285 | 0.807869 | 4.09E-50 | -1.69E-46 |
| MS.gene055603 | MS.gene24285 | 0.806091 | 9.73E-50 | -1.69E-46 |
| MS.gene05636 | MS.gene24285 | 0.804638 | 1.96E-49 | -1.69E-46 |
| MS.gene056403 | MS.gene24285 | 0.821954 | 3.07E-53 | -1.69E-46 |
| MS.gene056605 | MS.gene24285 | 0.810777 | 9.74E-51 | -1.69E-46 |
| MS.gene056776 | MS.gene24285 | 0.802068 | 6.69E-49 | -1.69E-46 |
| MS.gene056786 | MS.gene24285 | 0.803391 | 3.57E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene24285 | 0.844673 | 6.53E-59 | -1.69E-46 |
| MS.gene057102 | MS.gene24285 | 0.823687 | 1.21E-53 | -1.69E-46 |
| MS.gene057155 | MS.gene24285 | 0.856353 | 3.41E-62 | -1.69E-46 |
| MS.gene057622 | MS.gene24285 | 0.820252 | 7.57E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene24285 | 0.84282 | 2.05E-58 | -1.69E-46 |
| MS.gene058159 | MS.gene24285 | 0.815048 | 1.13E-51 | -1.69E-46 |
| MS.gene058429 | MS.gene24285 | 0.802981 | 4.34E-49 | -1.69E-46 |
| MS.gene058592 | MS.gene24285 | 0.803383 | 3.58E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene24285 | 0.810198 | 1.30E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene24285 | -0.819527 | 1.11E-52 | -1.69E-46 |
| MS.gene060012 | MS.gene24285 | 0.807005 | 6.24E-50 | -1.69E-46 |
| MS.gene06011 | MS.gene24285 | 0.824553 | 7.58E-54 | -1.69E-46 |
| MS.gene060259 | MS.gene24285 | 0.834729 | 2.53E-56 | -1.69E-46 |
| MS.gene060412 | MS.gene24285 | 0.82802 | 1.13E-54 | -1.69E-46 |
| MS.gene060932 | MS.gene24285 | 0.806193 | 9.27E-50 | -1.69E-46 |
| MS.gene060937 | MS.gene24285 | 0.80857 | 2.90E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATTGAGTACTACTCTTTA+TGG | 0.120660 | 1.1:+2087483 | None:intergenic |
| TATTGAGTACTACTCTTTAT+GGG | 0.330343 | 1.1:+2087484 | None:intergenic |
| CAAGACACAGACACACCTTT+TGG | 0.331421 | 1.1:+2087538 | None:intergenic |
| GAGATTCAACTTGTTCCAAA+AGG | 0.436800 | 1.1:-2087553 | MS.gene24285:CDS |
| TCGTAAGATTGTTGTGAAGA+TGG | 0.478593 | 1.1:-2087701 | MS.gene24285:CDS |
| CTTGTAGAAGCAGCAGAGCT+AGG | 0.495428 | 1.1:-2087519 | MS.gene24285:CDS |
| GGTTCTATGAAGAAAGAAGA+AGG | 0.541223 | 1.1:-2087634 | MS.gene24285:CDS |
| ATCAGAAGTAGAAGCATGCA+TGG | 0.580042 | 1.1:-2087599 | MS.gene24285:CDS |
| TCACAACAACAAGAAGAAGA+AGG | 0.587622 | 1.1:-2087655 | MS.gene24285:CDS |
| ATGCATGGTTAGAAAAGAAG+AGG | 0.601267 | 1.1:-2087584 | MS.gene24285:CDS |
| TGCATGGTTAGAAAAGAAGA+GGG | 0.637344 | 1.1:-2087583 | MS.gene24285:CDS |
| ATCTTCACAACAATCTTACG+AGG | 0.649549 | 1.1:+2087703 | None:intergenic |
| ATGGTTAGAAAAGAAGAGGG+AGG | 0.656282 | 1.1:-2087580 | MS.gene24285:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAAACAAAGGAAATTT+GGG | - | chr1.1:2087497-2087516 | MS.gene24285:CDS | 20.0% |
| ! | CACAAAAAACAAAGGAAATT+TGG | - | chr1.1:2087496-2087515 | MS.gene24285:CDS | 25.0% |
| ! | TAAGAAACCACAAAAAACAA+AGG | - | chr1.1:2087488-2087507 | MS.gene24285:CDS | 25.0% |
| ! | TATTGAGTACTACTCTTTAT+GGG | + | chr1.1:2087816-2087835 | None:intergenic | 25.0% |
| ! | TTATTGAGTACTACTCTTTA+TGG | + | chr1.1:2087817-2087836 | None:intergenic | 25.0% |
| !!! | CAAATTTCCTTTGTTTTTTG+TGG | + | chr1.1:2087498-2087517 | None:intergenic | 25.0% |
| ATCTTCACAACAATCTTACG+AGG | + | chr1.1:2087597-2087616 | None:intergenic | 35.0% | |
| ATGCATGGTTAGAAAAGAAG+AGG | - | chr1.1:2087713-2087732 | MS.gene24285:CDS | 35.0% | |
| GGTTCTATGAAGAAAGAAGA+AGG | - | chr1.1:2087663-2087682 | MS.gene24285:CDS | 35.0% | |
| TCACAACAACAAGAAGAAGA+AGG | - | chr1.1:2087642-2087661 | MS.gene24285:CDS | 35.0% | |
| TCGTAAGATTGTTGTGAAGA+TGG | - | chr1.1:2087596-2087615 | MS.gene24285:CDS | 35.0% | |
| TGCATGGTTAGAAAAGAAGA+GGG | - | chr1.1:2087714-2087733 | MS.gene24285:CDS | 35.0% | |
| ! | GAGATTCAACTTGTTCCAAA+AGG | - | chr1.1:2087744-2087763 | MS.gene24285:CDS | 35.0% |
| ATGGTTAGAAAAGAAGAGGG+AGG | - | chr1.1:2087717-2087736 | MS.gene24285:CDS | 40.0% | |
| ! | ATCAGAAGTAGAAGCATGCA+TGG | - | chr1.1:2087698-2087717 | MS.gene24285:CDS | 40.0% |
| CCTCAACCTACCAGAAAAAG+AGG | - | chr1.1:2087567-2087586 | MS.gene24285:CDS | 45.0% | |
| ! | ACGAGGTCTACCTCTTTTTC+TGG | + | chr1.1:2087580-2087599 | None:intergenic | 45.0% |
| ! | CAAGACACAGACACACCTTT+TGG | + | chr1.1:2087762-2087781 | None:intergenic | 45.0% |
| !! | CCTCTTTTTCTGGTAGGTTG+AGG | + | chr1.1:2087570-2087589 | None:intergenic | 45.0% |
| !! | GGTCTACCTCTTTTTCTGGT+AGG | + | chr1.1:2087576-2087595 | None:intergenic | 45.0% |
| CTTGTAGAAGCAGCAGAGCT+AGG | - | chr1.1:2087778-2087797 | MS.gene24285:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 2087483 | 2087836 | 2087483 | ID=MS.gene24285 |
| chr1.1 | mRNA | 2087483 | 2087836 | 2087483 | ID=MS.gene24285.t1;Parent=MS.gene24285 |
| chr1.1 | exon | 2087483 | 2087836 | 2087483 | ID=MS.gene24285.t1.exon1;Parent=MS.gene24285.t1 |
| chr1.1 | CDS | 2087483 | 2087836 | 2087483 | ID=cds.MS.gene24285.t1;Parent=MS.gene24285.t1 |
| Gene Sequence |
| Protein sequence |
