Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24846.t1 | XP_013458440.1 | 91.6 | 178 | 15 | 0 | 6 | 183 | 97 | 274 | 4.20E-78 | 300.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24846.t1 | A0A072UT04 | 91.6 | 178 | 15 | 0 | 6 | 183 | 97 | 274 | 3.1e-78 | 300.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049580 | MS.gene24846 | 0.818804 | 1.62E-52 | -1.69E-46 |
| MS.gene049821 | MS.gene24846 | 0.815194 | 1.05E-51 | -1.69E-46 |
| MS.gene049824 | MS.gene24846 | 0.842397 | 2.65E-58 | -1.69E-46 |
| MS.gene049825 | MS.gene24846 | 0.85533 | 6.78E-62 | -1.69E-46 |
| MS.gene049873 | MS.gene24846 | 0.838225 | 3.26E-57 | -1.69E-46 |
| MS.gene050277 | MS.gene24846 | 0.83032 | 3.14E-55 | -1.69E-46 |
| MS.gene050303 | MS.gene24846 | 0.816409 | 5.61E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene24846 | 0.832251 | 1.05E-55 | -1.69E-46 |
| MS.gene051330 | MS.gene24846 | 0.808128 | 3.61E-50 | -1.69E-46 |
| MS.gene051700 | MS.gene24846 | 0.840818 | 6.92E-58 | -1.69E-46 |
| MS.gene051746 | MS.gene24846 | 0.806189 | 9.28E-50 | -1.69E-46 |
| MS.gene051791 | MS.gene24846 | 0.816644 | 4.97E-52 | -1.69E-46 |
| MS.gene05188 | MS.gene24846 | 0.821787 | 3.35E-53 | -1.69E-46 |
| MS.gene051902 | MS.gene24846 | 0.812353 | 4.42E-51 | -1.69E-46 |
| MS.gene051903 | MS.gene24846 | 0.825397 | 4.79E-54 | -1.69E-46 |
| MS.gene052133 | MS.gene24846 | 0.813646 | 2.30E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene24846 | 0.804976 | 1.67E-49 | -1.69E-46 |
| MS.gene052947 | MS.gene24846 | 0.862711 | 4.18E-64 | -1.69E-46 |
| MS.gene053122 | MS.gene24846 | 0.809037 | 2.31E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene24846 | 0.810777 | 9.74E-51 | -1.69E-46 |
| MS.gene053294 | MS.gene24846 | 0.819382 | 1.20E-52 | -1.69E-46 |
| MS.gene053599 | MS.gene24846 | 0.800128 | 1.67E-48 | -1.69E-46 |
| MS.gene05364 | MS.gene24846 | 0.822244 | 2.63E-53 | -1.69E-46 |
| MS.gene055424 | MS.gene24846 | 0.807397 | 5.16E-50 | -1.69E-46 |
| MS.gene055468 | MS.gene24846 | 0.817112 | 3.91E-52 | -1.69E-46 |
| MS.gene055555 | MS.gene24846 | 0.851868 | 6.71E-61 | -1.69E-46 |
| MS.gene055580 | MS.gene24846 | -0.824523 | 7.71E-54 | -1.69E-46 |
| MS.gene055808 | MS.gene24846 | 0.851362 | 9.32E-61 | -1.69E-46 |
| MS.gene056043 | MS.gene24846 | -0.826974 | 2.02E-54 | -1.69E-46 |
| MS.gene056075 | MS.gene24846 | 0.814166 | 1.77E-51 | -1.69E-46 |
| MS.gene056091 | MS.gene24846 | 0.854639 | 1.08E-61 | -1.69E-46 |
| MS.gene056215 | MS.gene24846 | 0.816017 | 6.87E-52 | -1.69E-46 |
| MS.gene056631 | MS.gene24846 | 0.823919 | 1.07E-53 | -1.69E-46 |
| MS.gene05689 | MS.gene24846 | 0.808145 | 3.58E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene24846 | 0.802002 | 6.90E-49 | -1.69E-46 |
| MS.gene057094 | MS.gene24846 | 0.819272 | 1.27E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene24846 | 0.814111 | 1.82E-51 | -1.69E-46 |
| MS.gene057705 | MS.gene24846 | 0.854989 | 8.52E-62 | -1.69E-46 |
| MS.gene058074 | MS.gene24846 | 0.813059 | 3.10E-51 | -1.69E-46 |
| MS.gene058238 | MS.gene24846 | 0.800195 | 1.62E-48 | -1.69E-46 |
| MS.gene058852 | MS.gene24846 | 0.826464 | 2.67E-54 | -1.69E-46 |
| MS.gene05953 | MS.gene24846 | 0.813792 | 2.14E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene24846 | 0.834233 | 3.37E-56 | -1.69E-46 |
| MS.gene06001 | MS.gene24846 | 0.844855 | 5.84E-59 | -1.69E-46 |
| MS.gene06011 | MS.gene24846 | 0.812801 | 3.53E-51 | -1.69E-46 |
| MS.gene060365 | MS.gene24846 | 0.830156 | 3.44E-55 | -1.69E-46 |
| MS.gene060429 | MS.gene24846 | 0.862592 | 4.54E-64 | -1.69E-46 |
| MS.gene060586 | MS.gene24846 | 0.811542 | 6.65E-51 | -1.69E-46 |
| MS.gene060587 | MS.gene24846 | 0.801127 | 1.04E-48 | -1.69E-46 |
| MS.gene060763 | MS.gene24846 | 0.828195 | 1.03E-54 | -1.69E-46 |
| MS.gene060917 | MS.gene24846 | 0.808558 | 2.92E-50 | -1.69E-46 |
| MS.gene061266 | MS.gene24846 | 0.809695 | 1.67E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24846.t1 | MTR_4g125720 | 90.556 | 180 | 17 | 0 | 4 | 183 | 95 | 274 | 8.26e-112 | 320 |
| MS.gene24846.t1 | MTR_4g125720 | 90.141 | 142 | 14 | 0 | 4 | 145 | 95 | 236 | 2.61e-86 | 254 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24846.t1 | AT2G19385 | 37.975 | 158 | 56 | 5 | 4 | 149 | 95 | 222 | 3.29e-15 | 72.0 |
Find 36 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACATCAGTTGACGTGGAAAA+TGG | 0.191316 | 8.4:-9213659 | MS.gene24846:CDS |
| GGAAAATGGGGAAGTATTTC+AGG | 0.217234 | 8.4:-9213645 | MS.gene24846:CDS |
| AAACTTCATTCACCGGTTTC+AGG | 0.267736 | 8.4:+9213750 | None:intergenic |
| CATCAGTTGACGTGGAAAAT+GGG | 0.380294 | 8.4:-9213658 | MS.gene24846:CDS |
| TAGATTCCTGCAGTGCTTTG+AGG | 0.398509 | 8.4:+9213300 | None:intergenic |
| GAGGAAACATGAAGCATTAG+AGG | 0.399707 | 8.4:-9213711 | MS.gene24846:CDS |
| TTCTTCATCTTCAATCCATC+AGG | 0.401465 | 8.4:+9213338 | None:intergenic |
| AATTCAACATACAAATTTCA+AGG | 0.401997 | 8.4:+9213571 | None:intergenic |
| TCAGAAGACTCGTGCAACTT+TGG | 0.435496 | 8.4:+9213782 | None:intergenic |
| GCTTCGAAGCATGGAACGCT+CGG | 0.436244 | 8.4:+9213903 | None:intergenic |
| TCCACTGCATCCTTTGCATC+AGG | 0.440074 | 8.4:+9213851 | None:intergenic |
| CAGGCGCAAAATTATCTGTC+TGG | 0.451138 | 8.4:+9213870 | None:intergenic |
| GGAGACGGGTTCTAATGATA+TGG | 0.458688 | 8.4:-9213831 | MS.gene24846:CDS |
| CAAGAGAGAGGAAAATAAAG+TGG | 0.462140 | 8.4:-9213613 | MS.gene24846:CDS |
| TGAACAAAATAACCTGAAAC+CGG | 0.469254 | 8.4:-9213762 | MS.gene24846:CDS |
| TACTGGTGGCAGCATCCTGA+TGG | 0.473972 | 8.4:-9213353 | MS.gene24846:intron |
| GCAAAGGATGCAGTGGAGAC+GGG | 0.502647 | 8.4:-9213845 | MS.gene24846:CDS |
| TGCAAAGGATGCAGTGGAGA+CGG | 0.510937 | 8.4:-9213846 | MS.gene24846:CDS |
| CTTCGAAGCATGGAACGCTC+GGG | 0.518211 | 8.4:+9213904 | None:intergenic |
| TCGGAAGAAACTTCATTCAC+CGG | 0.522070 | 8.4:+9213743 | None:intergenic |
| CTAGTGGTTGCTTCGAAGCA+TGG | 0.522690 | 8.4:+9213894 | None:intergenic |
| GAGCGAGGCGATTGAGCAGA+AGG | 0.538864 | 8.4:-9213252 | None:intergenic |
| AAATTATCTGTCTGGACTAG+TGG | 0.555342 | 8.4:+9213878 | None:intergenic |
| AGTTGTCCTCAAAGCACTGC+AGG | 0.573521 | 8.4:-9213306 | MS.gene24846:CDS |
| GAAAATGGGGAAGTATTTCA+GGG | 0.576073 | 8.4:-9213644 | MS.gene24846:CDS |
| ATGCTGAAGGAAAGAAACAC+GGG | 0.581891 | 8.4:-9213934 | MS.gene24846:CDS |
| GCACTTCTTCTACATGCTGA+AGG | 0.586895 | 8.4:-9213947 | MS.gene24846:CDS |
| ATCAGTTGACGTGGAAAATG+GGG | 0.599482 | 8.4:-9213657 | MS.gene24846:CDS |
| AGGAAACATGAAGCATTAGA+GGG | 0.613312 | 8.4:-9213710 | MS.gene24846:CDS |
| GGAAGTATTTCAGGGCGAGA+AGG | 0.613916 | 8.4:-9213636 | MS.gene24846:CDS |
| AGGGCGAGAAGGCAAGAGAG+AGG | 0.620409 | 8.4:-9213625 | MS.gene24846:CDS |
| CATGCTGAAGGAAAGAAACA+CGG | 0.639025 | 8.4:-9213935 | MS.gene24846:CDS |
| GCCTGATGCAAAGGATGCAG+TGG | 0.663160 | 8.4:-9213852 | MS.gene24846:CDS |
| GAATGACACATCAGTTGACG+TGG | 0.669822 | 8.4:-9213666 | MS.gene24846:CDS |
| AAATGAAAATGAATTGAGCG+AGG | 0.672838 | 8.4:-9213267 | MS.gene24846:CDS |
| TGCTGAAGGAAAGAAACACG+GGG | 0.771538 | 8.4:-9213933 | MS.gene24846:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATCTATATATGTTATAGT+GGG | - | chr8.4:9213733-9213752 | MS.gene24846:CDS | 15.0% |
| !! | TTTATCTATATATGTTATAG+TGG | - | chr8.4:9213732-9213751 | MS.gene24846:CDS | 15.0% |
| !! | AATTCAACATACAAATTTCA+AGG | + | chr8.4:9213683-9213702 | None:intergenic | 20.0% |
| !!! | GAAATTTGTATGTTGAATTT+TGG | - | chr8.4:9213684-9213703 | MS.gene24846:CDS | 20.0% |
| ! | ATTACAGCAGGAAAATTATA+TGG | + | chr8.4:9213865-9213884 | None:intergenic | 25.0% |
| ! | TTACAGCAGGAAAATTATAT+GGG | + | chr8.4:9213864-9213883 | None:intergenic | 25.0% |
| !! | ATGTTTCCTCTTTTTCTTTT+CGG | + | chr8.4:9213530-9213549 | None:intergenic | 25.0% |
| !!! | TAATCTGCTTATTGTTTTAC+TGG | - | chr8.4:9213881-9213900 | MS.gene24846:CDS | 25.0% |
| AAATGAAAATGAATTGAGCG+AGG | - | chr8.4:9213984-9214003 | MS.gene24846:CDS | 30.0% | |
| GATAAAGCAGAAAACAAGAA+AGG | + | chr8.4:9213718-9213737 | None:intergenic | 30.0% | |
| TACAGCAGGAAAATTATATG+GGG | + | chr8.4:9213863-9213882 | None:intergenic | 30.0% | |
| TATAGTGATATAGAGATAGC+TGG | - | chr8.4:9213782-9213801 | MS.gene24846:CDS | 30.0% | |
| TGAACAAAATAACCTGAAAC+CGG | - | chr8.4:9213489-9213508 | MS.gene24846:intron | 30.0% | |
| TTATCTGTTTATTCTGAGCA+TGG | - | chr8.4:9213759-9213778 | MS.gene24846:CDS | 30.0% | |
| TTTCTTCCGAAAAGAAAAAG+AGG | - | chr8.4:9213521-9213540 | MS.gene24846:intron | 30.0% | |
| AACAATAAGCAGATTACAGC+AGG | + | chr8.4:9213877-9213896 | None:intergenic | 35.0% | |
| CAAGAGAGAGGAAAATAAAG+TGG | - | chr8.4:9213638-9213657 | MS.gene24846:CDS | 35.0% | |
| TTCTTCATCTTCAATCCATC+AGG | + | chr8.4:9213916-9213935 | None:intergenic | 35.0% | |
| ! | AAATTATCTGTCTGGACTAG+TGG | + | chr8.4:9213376-9213395 | None:intergenic | 35.0% |
| ! | AGGAAACATGAAGCATTAGA+GGG | - | chr8.4:9213541-9213560 | MS.gene24846:intron | 35.0% |
| !! | GAAAATGGGGAAGTATTTCA+GGG | - | chr8.4:9213607-9213626 | MS.gene24846:CDS | 35.0% |
| !! | TCTGCTTATTGTTTTACTGG+TGG | - | chr8.4:9213884-9213903 | MS.gene24846:CDS | 35.0% |
| ACATCAGTTGACGTGGAAAA+TGG | - | chr8.4:9213592-9213611 | MS.gene24846:CDS | 40.0% | |
| ATCAGTTGACGTGGAAAATG+GGG | - | chr8.4:9213594-9213613 | MS.gene24846:CDS | 40.0% | |
| ATGCTGAAGGAAAGAAACAC+GGG | - | chr8.4:9213317-9213336 | MS.gene24846:CDS | 40.0% | |
| CATCAGTTGACGTGGAAAAT+GGG | - | chr8.4:9213593-9213612 | MS.gene24846:CDS | 40.0% | |
| CATGCTGAAGGAAAGAAACA+CGG | - | chr8.4:9213316-9213335 | MS.gene24846:CDS | 40.0% | |
| TCGGAAGAAACTTCATTCAC+CGG | + | chr8.4:9213511-9213530 | None:intergenic | 40.0% | |
| ! | GAGATAGCTGGTGACTTTTA+AGG | - | chr8.4:9213794-9213813 | MS.gene24846:CDS | 40.0% |
| ! | GAGGAAACATGAAGCATTAG+AGG | - | chr8.4:9213540-9213559 | MS.gene24846:intron | 40.0% |
| ! | TAATTTTGCGCCTGATGCAA+AGG | - | chr8.4:9213390-9213409 | MS.gene24846:intron | 40.0% |
| !! | AAACTTCATTCACCGGTTTC+AGG | + | chr8.4:9213504-9213523 | None:intergenic | 40.0% |
| !! | GGAAAATGGGGAAGTATTTC+AGG | - | chr8.4:9213606-9213625 | MS.gene24846:CDS | 40.0% |
| CAGGCGCAAAATTATCTGTC+TGG | + | chr8.4:9213384-9213403 | None:intergenic | 45.0% | |
| GAATGACACATCAGTTGACG+TGG | - | chr8.4:9213585-9213604 | MS.gene24846:CDS | 45.0% | |
| GCACTTCTTCTACATGCTGA+AGG | - | chr8.4:9213304-9213323 | MS.gene24846:CDS | 45.0% | |
| TAGATTCCTGCAGTGCTTTG+AGG | + | chr8.4:9213954-9213973 | None:intergenic | 45.0% | |
| TCAGAAGACTCGTGCAACTT+TGG | + | chr8.4:9213472-9213491 | None:intergenic | 45.0% | |
| TGCTGAAGGAAAGAAACACG+GGG | - | chr8.4:9213318-9213337 | MS.gene24846:CDS | 45.0% | |
| ! | GGAGACGGGTTCTAATGATA+TGG | - | chr8.4:9213420-9213439 | MS.gene24846:intron | 45.0% |
| CTAGTGGTTGCTTCGAAGCA+TGG | + | chr8.4:9213360-9213379 | None:intergenic | 50.0% | |
| TCCACTGCATCCTTTGCATC+AGG | + | chr8.4:9213403-9213422 | None:intergenic | 50.0% | |
| TGCAAAGGATGCAGTGGAGA+CGG | - | chr8.4:9213405-9213424 | MS.gene24846:intron | 50.0% | |
| !! | AGTTGTCCTCAAAGCACTGC+AGG | - | chr8.4:9213945-9213964 | MS.gene24846:CDS | 50.0% |
| !! | GGAAGTATTTCAGGGCGAGA+AGG | - | chr8.4:9213615-9213634 | MS.gene24846:CDS | 50.0% |
| CTTCGAAGCATGGAACGCTC+GGG | + | chr8.4:9213350-9213369 | None:intergenic | 55.0% | |
| GCAAAGGATGCAGTGGAGAC+GGG | - | chr8.4:9213406-9213425 | MS.gene24846:intron | 55.0% | |
| GCCTGATGCAAAGGATGCAG+TGG | - | chr8.4:9213399-9213418 | MS.gene24846:intron | 55.0% | |
| GCTTCGAAGCATGGAACGCT+CGG | + | chr8.4:9213351-9213370 | None:intergenic | 55.0% | |
| TACTGGTGGCAGCATCCTGA+TGG | - | chr8.4:9213898-9213917 | MS.gene24846:CDS | 55.0% | |
| AGGGCGAGAAGGCAAGAGAG+AGG | - | chr8.4:9213626-9213645 | MS.gene24846:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9213253 | 9214020 | 9213253 | ID=MS.gene24846 |
| chr8.4 | mRNA | 9213253 | 9214020 | 9213253 | ID=MS.gene24846.t1;Parent=MS.gene24846 |
| chr8.4 | exon | 9213583 | 9214020 | 9213583 | ID=MS.gene24846.t1.exon1;Parent=MS.gene24846.t1 |
| chr8.4 | CDS | 9213583 | 9214020 | 9213583 | ID=cds.MS.gene24846.t1;Parent=MS.gene24846.t1 |
| chr8.4 | exon | 9213253 | 9213363 | 9213253 | ID=MS.gene24846.t1.exon2;Parent=MS.gene24846.t1 |
| chr8.4 | CDS | 9213253 | 9213363 | 9213253 | ID=cds.MS.gene24846.t1;Parent=MS.gene24846.t1 |
| Gene Sequence |
| Protein sequence |
