AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25816


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25816.t1 MTR_3g080100 96.078 255 10 0 1 255 1 255 0.0 499
MS.gene25816.t1 MTR_3g080100 96.296 216 8 0 40 255 1 216 3.29e-153 425
MS.gene25816.t1 MTR_8g089895 37.785 307 134 7 1 255 1 302 5.26e-54 177
MS.gene25816.t1 MTR_3g086790 39.731 297 133 9 1 255 1 293 1.28e-47 160
MS.gene25816.t1 MTR_6g011610 46.465 198 91 4 42 238 82 265 4.86e-41 142
MS.gene25816.t1 MTR_6g011610 46.465 198 91 4 42 238 63 246 5.51e-41 141
MS.gene25816.t1 MTR_8g468210 57.724 123 51 1 46 168 64 185 2.23e-38 135
MS.gene25816.t1 MTR_5g039000 57.600 125 52 1 44 168 62 185 4.23e-38 135
MS.gene25816.t1 MTR_5g038280 60.000 110 44 0 47 156 75 184 7.25e-37 132
MS.gene25816.t1 MTR_6g007647 64.286 98 35 0 58 155 32 129 9.85e-35 125
MS.gene25816.t1 MTR_8g469430 60.577 104 41 0 59 162 89 192 1.23e-34 126
MS.gene25816.t1 MTR_6g007647 64.286 98 35 0 58 155 66 163 2.44e-34 125
MS.gene25816.t1 MTR_4g107650 61.905 105 40 0 58 162 94 198 4.92e-34 125
MS.gene25816.t1 MTR_7g010020 51.938 129 58 2 47 174 71 196 8.19e-33 120
MS.gene25816.t1 MTR_7g103340 47.761 134 68 2 52 185 56 187 6.73e-30 112
MS.gene25816.t1 MTR_1g061660 49.550 111 56 0 58 168 58 168 1.49e-25 100
MS.gene25816.t1 MTR_8g026960 51.163 86 42 0 66 151 41 126 4.60e-24 97.1
MS.gene25816.t1 MTR_3g092150 48.352 91 47 0 61 151 40 130 3.56e-23 94.7
MS.gene25816.t1 MTR_4g097600 47.748 111 49 2 43 153 113 214 1.46e-20 88.2
MS.gene25816.t1 MTR_4g100550 46.667 105 49 1 49 153 110 207 4.65e-20 87.0
MS.gene25816.t1 MTR_5g019680 44.068 118 54 1 51 168 45 150 2.30e-19 84.0
MS.gene25816.t1 MTR_5g019650 44.167 120 58 3 51 168 45 157 9.67e-19 82.4
MS.gene25816.t1 MTR_1g054285 48.113 106 46 2 48 153 145 241 2.31e-18 82.8
MS.gene25816.t1 MTR_2g061030 42.222 135 58 3 19 153 173 287 2.75e-18 83.2
MS.gene25816.t1 MTR_7g093430 47.170 106 47 2 48 153 151 247 3.00e-18 82.4
MS.gene25816.t1 MTR_4g126900 39.130 138 74 2 19 153 45 175 9.72e-18 79.7
MS.gene25816.t1 MTR_5g013010 42.857 133 56 3 21 152 112 225 2.40e-17 80.1
MS.gene25816.t1 MTR_5g014890 46.226 106 49 2 48 153 113 210 4.39e-17 79.0
MS.gene25816.t1 MTR_1g017090 46.316 95 44 1 59 153 130 217 4.73e-16 76.3
MS.gene25816.t1 MTR_8g006705 43.363 113 59 2 59 170 146 254 4.78e-16 76.6
MS.gene25816.t1 MTR_3g103590 39.130 138 67 4 57 186 143 271 1.40e-14 72.4
MS.gene25816.t1 MTR_4g084750 44.211 95 46 1 59 153 65 152 2.11e-14 70.1
MS.gene25816.t1 MTR_2g038625 44.211 95 46 1 59 153 82 169 3.86e-13 67.0
MS.gene25816.t1 MTR_2g038580 44.211 95 46 1 59 153 82 169 3.86e-13 67.0
MS.gene25816.t1 MTR_5g013010 43.956 91 35 2 21 110 112 187 2.72e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25816.t1 AT5G65310 53.571 168 65 4 1 155 1 168 4.77e-49 164
MS.gene25816.t1 AT5G65310 59.441 143 47 2 24 155 8 150 1.11e-48 162
MS.gene25816.t1 AT4G40060 35.390 308 122 9 1 255 1 284 2.47e-45 154
MS.gene25816.t1 AT2G22430 49.057 159 75 2 1 155 2 158 1.92e-42 147
MS.gene25816.t1 AT1G69780 60.163 123 48 1 46 168 72 193 3.87e-40 140
MS.gene25816.t1 AT5G15150 58.594 128 49 3 42 166 95 221 1.96e-38 136
MS.gene25816.t1 AT1G26960 44.000 200 97 5 41 235 53 242 1.57e-37 132
MS.gene25816.t1 AT3G01220 44.086 186 94 4 44 224 70 250 3.47e-36 130
MS.gene25816.t1 AT3G01470 60.000 100 40 0 56 155 65 164 4.05e-33 121
MS.gene25816.t1 AT3G61890 39.161 143 82 2 62 202 33 172 1.63e-27 106
MS.gene25816.t1 AT1G27045 54.545 99 45 0 57 155 66 164 5.73e-27 104
MS.gene25816.t1 AT1G27045 54.545 99 45 0 57 155 15 113 8.80e-27 102
MS.gene25816.t1 AT1G27045 54.545 99 45 0 57 155 23 121 9.21e-27 102
MS.gene25816.t1 AT1G27045 54.545 99 45 0 57 155 23 121 9.21e-27 102
MS.gene25816.t1 AT2G46680 50.538 93 46 0 60 152 33 125 3.03e-26 103
MS.gene25816.t1 AT1G27045 53.684 95 44 0 57 151 66 160 2.24e-25 100
MS.gene25816.t1 AT2G46680 48.958 96 48 1 60 154 33 128 6.52e-25 99.8
MS.gene25816.t1 AT2G18550 39.521 167 78 4 59 217 61 212 1.43e-22 92.8
MS.gene25816.t1 AT5G03790 50.000 92 46 0 59 150 77 168 1.13e-21 90.5
MS.gene25816.t1 AT4G36740 47.788 113 55 1 52 160 49 161 3.40e-21 89.0
MS.gene25816.t1 AT4G36740 47.788 113 55 1 52 160 48 160 3.51e-21 89.0
MS.gene25816.t1 AT5G03790 52.439 82 39 0 59 140 77 158 6.80e-21 87.4
MS.gene25816.t1 AT2G36610 45.545 101 47 1 58 150 70 170 1.02e-20 87.0
MS.gene25816.t1 AT5G66700 35.429 175 101 5 46 217 60 225 3.83e-20 86.3
MS.gene25816.t1 AT5G53980 42.268 97 56 0 59 155 11 107 4.27e-19 81.6
MS.gene25816.t1 AT2G01430 37.589 141 80 2 45 184 125 258 5.17e-19 84.3
MS.gene25816.t1 AT5G47370 42.424 132 63 2 21 152 101 219 4.39e-18 82.0
MS.gene25816.t1 AT4G17460 42.063 126 62 3 32 153 104 222 1.34e-17 80.5
MS.gene25816.t1 AT5G06710 39.850 133 75 2 20 152 152 279 1.67e-17 80.9
MS.gene25816.t1 AT4G37790 42.241 116 59 2 38 153 106 213 8.34e-17 78.2
MS.gene25816.t1 AT2G44910 46.316 95 44 1 59 153 163 250 6.42e-16 76.3
MS.gene25816.t1 AT3G60390 42.593 108 58 1 45 152 148 251 3.24e-15 74.3
MS.gene25816.t1 AT2G22800 39.837 123 72 2 48 169 101 222 5.04e-15 73.2
MS.gene25816.t1 AT4G16780 43.689 103 50 2 51 153 122 216 8.02e-15 72.8
MS.gene25816.t1 AT2G01430 43.529 85 44 1 45 125 125 209 1.22e-12 65.5
MS.gene25816.t1 AT1G70920 38.525 122 73 1 23 144 5 124 4.51e-12 63.2
MS.gene25816.t1 AT1G70920 38.525 122 73 1 23 144 35 154 4.79e-12 63.9
MS.gene25816.t1 AT5G06710 39.560 91 54 1 20 110 152 241 6.09e-12 64.3

Find 36 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCAAGTTCAATGATGAATT+TGG 0.158147 3.2:-70300739 MS.gene25816:CDS
TCGTTCGTGCTCGACGATTT+TGG 0.160811 3.2:+70301137 None:intergenic
CTTTCTATACCTCTTGAAAT+TGG 0.202435 3.2:+70300843 None:intergenic
AACCTTAAGAACATCAAAAT+TGG 0.244715 3.2:+70301075 None:intergenic
TTGAAGTTAGCTGAAGAATT+AGG 0.251335 3.2:-70301190 MS.gene25816:CDS
AACCTAGACAAGTTTCAATT+TGG 0.262579 3.2:-70301162 MS.gene25816:CDS
TAATTCAAGTGGTGTGATTA+AGG 0.320954 3.2:-70300805 MS.gene25816:CDS
ATAATCTCTTTCCAATTGTT+TGG 0.328373 3.2:+70301111 None:intergenic
TTTAGAAAAGAGCTTTGAAT+TGG 0.355867 3.2:-70301239 MS.gene25816:CDS
GATTCTCCTATATGTTTCAA+TGG 0.381447 3.2:-70300768 MS.gene25816:CDS
GTTCAATAACTCAGAAACTA+TGG 0.387793 3.2:-70301552 MS.gene25816:CDS
TCAGAATCAATTTATGAGAA+TGG 0.408136 3.2:-70300676 MS.gene25816:CDS
CATACCTTTGGATTGGTACA+TGG 0.415046 3.2:+70301517 None:intergenic
ACCAAATTCATCATTGAACT+TGG 0.453246 3.2:+70300738 None:intergenic
ACTTATACATACCTTTGGAT+TGG 0.465929 3.2:+70301510 None:intergenic
CTCAGTTGGTATTACAAAGT+AGG 0.474989 3.2:+70300594 None:intergenic
AAAGAACCATTGAAACATAT+AGG 0.475600 3.2:+70300762 None:intergenic
CTTTGAATTGGACAACAAGC+TGG 0.480175 3.2:-70301227 MS.gene25816:CDS
TCAGTTGGTATTACAAAGTA+GGG 0.482035 3.2:+70300595 None:intergenic
ACGAACGAAAACCAAACAAT+TGG 0.484787 3.2:-70301122 MS.gene25816:CDS
GAATGAGACTCTTACTAGAA+AGG 0.510168 3.2:-70301029 MS.gene25816:intron
TGCAAATGCAGGTGTGGATG+TGG 0.525574 3.2:-70301398 MS.gene25816:intron
CAAGAAGATAGCTATGAAGA+TGG 0.532967 3.2:-70301313 MS.gene25816:CDS
TGAAGATGGTAGTTCAATGT+TGG 0.535009 3.2:-70301299 MS.gene25816:CDS
AACCAAATTGAAACTTGTCT+AGG 0.545141 3.2:+70301160 None:intergenic
ATTTCAAGAGGTATAGAAAG+AGG 0.548836 3.2:-70300840 MS.gene25816:CDS
GATCAGAGGAATGAGACAAA+AGG 0.552396 3.2:-70301376 MS.gene25816:CDS
CTTTCCATGTACCAATCCAA+AGG 0.553684 3.2:-70301521 MS.gene25816:intron
TTGGAGAAGAAAAGAAGACT+TGG 0.569876 3.2:-70301280 MS.gene25816:CDS
GTTCATTATTATGATTCAAG+AGG 0.577370 3.2:-70300717 MS.gene25816:CDS
GTAGAATGTCCAATTTCAAG+AGG 0.608484 3.2:-70300852 MS.gene25816:CDS
GAATCAATTTATGAGAATGG+AGG 0.629666 3.2:-70300673 MS.gene25816:CDS
TCAAGAGGTATAGAAAGAGG+AGG 0.641487 3.2:-70300837 MS.gene25816:CDS
CAGTTGGTATTACAAAGTAG+GGG 0.656897 3.2:+70300596 None:intergenic
GGTTCAGAAAGTAATTCAAG+TGG 0.665455 3.2:-70300816 MS.gene25816:CDS
CAGGTGTGGATGTGGATCAG+AGG 0.671722 3.2:-70301390 MS.gene25816:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATACACACATAAATTA+TGG + chr3.2:70300723-70300742 None:intergenic 15.0%
!! ACACATAAATTATGGTAAAA+AGG + chr3.2:70300715-70300734 None:intergenic 20.0%
!! CACATAAATTATGGTAAAAA+GGG + chr3.2:70300714-70300733 None:intergenic 20.0%
!! TAATTACTTATACATACCTT+TGG + chr3.2:70300663-70300682 None:intergenic 20.0%
!!! AATTTATGTGTGTATTTTTG+TGG - chr3.2:70300724-70300743 MS.gene25816:CDS 20.0%
! AAAGAACCATTGAAACATAT+AGG + chr3.2:70301406-70301425 None:intergenic 25.0%
! AACCTTAAGAACATCAAAAT+TGG + chr3.2:70301093-70301112 None:intergenic 25.0%
! ATAATCTCTTTCCAATTGTT+TGG + chr3.2:70301057-70301076 None:intergenic 25.0%
! GTTCATTATTATGATTCAAG+AGG - chr3.2:70301448-70301467 MS.gene25816:intron 25.0%
! TCAGAATCAATTTATGAGAA+TGG - chr3.2:70301489-70301508 MS.gene25816:intron 25.0%
!!! TTTAGAAAAGAGCTTTGAAT+TGG - chr3.2:70300926-70300945 MS.gene25816:intron 25.0%
AACCAAATTGAAACTTGTCT+AGG + chr3.2:70301008-70301027 None:intergenic 30.0%
AACCTAGACAAGTTTCAATT+TGG - chr3.2:70301003-70301022 MS.gene25816:intron 30.0%
ACCAAATTCATCATTGAACT+TGG + chr3.2:70301430-70301449 None:intergenic 30.0%
ACTTATACATACCTTTGGAT+TGG + chr3.2:70300658-70300677 None:intergenic 30.0%
CTTTCTATACCTCTTGAAAT+TGG + chr3.2:70301325-70301344 None:intergenic 30.0%
GAATCAATTTATGAGAATGG+AGG - chr3.2:70301492-70301511 MS.gene25816:intron 30.0%
GATTCTCCTATATGTTTCAA+TGG - chr3.2:70301397-70301416 MS.gene25816:intron 30.0%
GTTCAATAACTCAGAAACTA+TGG - chr3.2:70300613-70300632 MS.gene25816:CDS 30.0%
TAATTCAAGTGGTGTGATTA+AGG - chr3.2:70301360-70301379 MS.gene25816:CDS 30.0%
TTGAAGTTAGCTGAAGAATT+AGG - chr3.2:70300975-70300994 MS.gene25816:intron 30.0%
! ATTTCAAGAGGTATAGAAAG+AGG - chr3.2:70301325-70301344 MS.gene25816:CDS 30.0%
!! GTCCAATTTTGATGTTCTTA+AGG - chr3.2:70301088-70301107 MS.gene25816:CDS 30.0%
ACGAACGAAAACCAAACAAT+TGG - chr3.2:70301043-70301062 MS.gene25816:CDS 35.0%
CAAGAAGATAGCTATGAAGA+TGG - chr3.2:70300852-70300871 MS.gene25816:CDS 35.0%
GAATGAGACTCTTACTAGAA+AGG - chr3.2:70301136-70301155 MS.gene25816:CDS 35.0%
GCCAAGTTCAATGATGAATT+TGG - chr3.2:70301426-70301445 MS.gene25816:intron 35.0%
GGTTCAGAAAGTAATTCAAG+TGG - chr3.2:70301349-70301368 MS.gene25816:CDS 35.0%
GTAGAATGTCCAATTTCAAG+AGG - chr3.2:70301313-70301332 MS.gene25816:CDS 35.0%
GTTTCATTCTTTGCAAATGC+AGG - chr3.2:70300756-70300775 MS.gene25816:CDS 35.0%
TGAAGATGGTAGTTCAATGT+TGG - chr3.2:70300866-70300885 MS.gene25816:CDS 35.0%
TTGGAGAAGAAAAGAAGACT+TGG - chr3.2:70300885-70300904 MS.gene25816:CDS 35.0%
! GCTAACTTCAATTTTCTCTC+TGG + chr3.2:70300966-70300985 None:intergenic 35.0%
ATTCTTTGCAAATGCAGGTG+TGG - chr3.2:70300761-70300780 MS.gene25816:CDS 40.0%
CTTTCCATGTACCAATCCAA+AGG - chr3.2:70300644-70300663 MS.gene25816:CDS 40.0%
CTTTGAATTGGACAACAAGC+TGG - chr3.2:70300938-70300957 MS.gene25816:intron 40.0%
GATCAGAGGAATGAGACAAA+AGG - chr3.2:70300789-70300808 MS.gene25816:CDS 40.0%
! TCAAGAGGTATAGAAAGAGG+AGG - chr3.2:70301328-70301347 MS.gene25816:CDS 40.0%
!! CATACCTTTGGATTGGTACA+TGG + chr3.2:70300651-70300670 None:intergenic 40.0%
TGCAAATGCAGGTGTGGATG+TGG - chr3.2:70300767-70300786 MS.gene25816:CDS 50.0%
! TCGTTCGTGCTCGACGATTT+TGG + chr3.2:70301031-70301050 None:intergenic 50.0%
CAGGTGTGGATGTGGATCAG+AGG - chr3.2:70300775-70300794 MS.gene25816:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 70300605 70301582 70300605 ID=MS.gene25816
chr3.2 mRNA 70300605 70301582 70300605 ID=MS.gene25816.t1;Parent=MS.gene25816
chr3.2 exon 70301522 70301582 70301522 ID=MS.gene25816.t1.exon1;Parent=MS.gene25816.t1
chr3.2 CDS 70301522 70301582 70301522 ID=cds.MS.gene25816.t1;Parent=MS.gene25816.t1
chr3.2 exon 70301030 70301409 70301030 ID=MS.gene25816.t1.exon2;Parent=MS.gene25816.t1
chr3.2 CDS 70301030 70301409 70301030 ID=cds.MS.gene25816.t1;Parent=MS.gene25816.t1
chr3.2 exon 70300605 70300931 70300605 ID=MS.gene25816.t1.exon3;Parent=MS.gene25816.t1
chr3.2 CDS 70300605 70300931 70300605 ID=cds.MS.gene25816.t1;Parent=MS.gene25816.t1
Gene Sequence

>MS.gene25816

ATGAAGAGGTTCAATAACTCAGAAACTATGGCTTCTTTGCTTTCCATGTACCAATCCAAAGGTATGTATAAGTAATTAAATTACTAATTGTTTCATTCTTCATATACCCTTTTTACCATAATTTATGTGTGTATTTTTGTGGTCTAATTATGTTTCATTCTTTGCAAATGCAGGTGTGGATGTGGATCAGAGGAATGAGACAAAAGGTTACAGTGAAGAATTTCAAGCAATGTTAGATAGATTAGAGCAAGAAGATAGCTATGAAGATGGTAGTTCAATGTTGGAGAAGAAAAGAAGACTTGGTTATGATCAAGTAAAAGCTTTAGAAAAGAGCTTTGAATTGGACAACAAGCTGGAGCCAGAGAGAAAATTGAAGTTAGCTGAAGAATTAGGCTTGCAACCTAGACAAGTTTCAATTTGGTTCCAAAATCGTCGAGCACGAACGAAAACCAAACAATTGGAAAGAGATTATAGTGTTCTTAAGTCCAATTTTGATGTTCTTAAGGTTGAGTATAGTACTCTTCAACAAGAGAATGAGACTCTTACTAGAAAGGTAATAATATATACATGTATTATTCATGATTTTTTATTTATTTATGTGAATAATGTTAATGTGTAATCTTAGTGTCTTTTTATGTGTGTGAGCTGCAGTTAAGAGAGTTGAAAGCGAAATTGTTAAGAGTTGAAAGCAATGAGTTAGAGAAAGAAGTAGAATGTCCAATTTCAAGAGGTATAGAAAGAGGAGGTTCAGAAAGTAATTCAAGTGGTGTGATTAAGGAAGAAAGTAATGTTGATTCTCCTATATGTTTCAATGGTTCTTTGCCAAGTTCAATGATGAATTTGGTTCATTATTATGATTCAAGAGGTTCTCAAGAAAATGTGTTTCAGAATCAATTTATGAGAATGGAGGAGCAGAATTTCTTAAGTAATAGTGAATTATTCAGTTTCTTTTCAGTTGATCAATCCCCTACTTTGTAA

Protein sequence

>MS.gene25816.t1

MKRFNNSETMASLLSMYQSKGVDVDQRNETKGYSEEFQAMLDRLEQEDSYEDGSSMLEKKRRLGYDQVKALEKSFELDNKLEPERKLKLAEELGLQPRQVSIWFQNRRARTKTKQLERDYSVLKSNFDVLKVEYSTLQQENETLTRKLRELKAKLLRVESNELEKEVECPISRGIERGGSESNSSGVIKEESNVDSPICFNGSLPSSMMNLVHYYDSRGSQENVFQNQFMRMEEQNFLSNSELFSFFSVDQSPTL