Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26958.t1 | XP_024638160.1 | 63.9 | 119 | 31 | 3 | 1 | 117 | 1 | 109 | 1.10E-18 | 102.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26958.t1 | I3SX70 | 63.9 | 119 | 31 | 3 | 1 | 117 | 1 | 109 | 7.8e-19 | 102.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene26958 | 0.836703 | 8.01E-57 | -1.69E-46 |
| MS.gene049419 | MS.gene26958 | -0.80391 | 2.78E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene26958 | 0.821298 | 4.35E-53 | -1.69E-46 |
| MS.gene04998 | MS.gene26958 | 0.826304 | 2.92E-54 | -1.69E-46 |
| MS.gene051676 | MS.gene26958 | 0.822982 | 1.77E-53 | -1.69E-46 |
| MS.gene051975 | MS.gene26958 | 0.824079 | 9.80E-54 | -1.69E-46 |
| MS.gene052434 | MS.gene26958 | 0.82261 | 2.16E-53 | -1.69E-46 |
| MS.gene052533 | MS.gene26958 | 0.80085 | 1.19E-48 | -1.69E-46 |
| MS.gene052685 | MS.gene26958 | 0.805521 | 1.28E-49 | -1.69E-46 |
| MS.gene052818 | MS.gene26958 | -0.816059 | 6.72E-52 | -1.69E-46 |
| MS.gene053298 | MS.gene26958 | 0.999123 | 2.33E-291 | -1.69E-46 |
| MS.gene053340 | MS.gene26958 | 0.827737 | 1.33E-54 | -1.69E-46 |
| MS.gene053786 | MS.gene26958 | 0.800853 | 1.19E-48 | -1.69E-46 |
| MS.gene053963 | MS.gene26958 | 0.870306 | 1.61E-66 | -1.69E-46 |
| MS.gene054222 | MS.gene26958 | 0.84819 | 7.18E-60 | -1.69E-46 |
| MS.gene054479 | MS.gene26958 | 0.813428 | 2.57E-51 | -1.69E-46 |
| MS.gene05469 | MS.gene26958 | 0.82541 | 4.76E-54 | -1.69E-46 |
| MS.gene054892 | MS.gene26958 | 0.822968 | 1.78E-53 | -1.69E-46 |
| MS.gene054893 | MS.gene26958 | 0.823881 | 1.09E-53 | -1.69E-46 |
| MS.gene05517 | MS.gene26958 | 0.801777 | 7.67E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene26958 | 0.803727 | 3.04E-49 | -1.69E-46 |
| MS.gene055603 | MS.gene26958 | 0.807789 | 4.26E-50 | -1.69E-46 |
| MS.gene05636 | MS.gene26958 | 0.804337 | 2.27E-49 | -1.69E-46 |
| MS.gene056403 | MS.gene26958 | 0.819734 | 9.94E-53 | -1.69E-46 |
| MS.gene056605 | MS.gene26958 | 0.810656 | 1.03E-50 | -1.69E-46 |
| MS.gene056786 | MS.gene26958 | 0.810005 | 1.43E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene26958 | 0.846208 | 2.51E-59 | -1.69E-46 |
| MS.gene057102 | MS.gene26958 | 0.82191 | 3.14E-53 | -1.69E-46 |
| MS.gene057155 | MS.gene26958 | 0.84782 | 9.08E-60 | -1.69E-46 |
| MS.gene057622 | MS.gene26958 | 0.812851 | 3.44E-51 | -1.69E-46 |
| MS.gene057999 | MS.gene26958 | 0.843287 | 1.54E-58 | -1.69E-46 |
| MS.gene058159 | MS.gene26958 | 0.820393 | 7.02E-53 | -1.69E-46 |
| MS.gene058429 | MS.gene26958 | 0.805369 | 1.38E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene26958 | 0.80593 | 1.05E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene26958 | -0.820067 | 8.34E-53 | -1.69E-46 |
| MS.gene060012 | MS.gene26958 | 0.810476 | 1.13E-50 | -1.69E-46 |
| MS.gene06011 | MS.gene26958 | 0.826503 | 2.62E-54 | -1.69E-46 |
| MS.gene060259 | MS.gene26958 | 0.83174 | 1.41E-55 | -1.69E-46 |
| MS.gene060412 | MS.gene26958 | 0.817527 | 3.15E-52 | -1.69E-46 |
| MS.gene060932 | MS.gene26958 | 0.806174 | 9.35E-50 | -1.69E-46 |
| MS.gene060937 | MS.gene26958 | 0.805113 | 1.56E-49 | -1.69E-46 |
| MS.gene061047 | MS.gene26958 | 0.801454 | 8.94E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATTGAGTACTACTCTTTA+TGG | 0.120660 | 1.3:+2842994 | None:intergenic |
| TATTGAGTACTACTCTTTAT+GGG | 0.330343 | 1.3:+2842995 | None:intergenic |
| CAAGACACAGACACACCTTT+TGG | 0.331421 | 1.3:+2843049 | None:intergenic |
| GAGATTCAACTTGTTCCAAA+AGG | 0.436800 | 1.3:-2843064 | MS.gene26958:CDS |
| TCGTAAGATTGTTGTGAAGA+TGG | 0.478593 | 1.3:-2843212 | MS.gene26958:CDS |
| CTTGTAGAAGCAGCAGAGCT+AGG | 0.495428 | 1.3:-2843030 | MS.gene26958:CDS |
| GGTTCTATGAAGAAAGAAGA+AGG | 0.541223 | 1.3:-2843145 | MS.gene26958:CDS |
| ATCAGAAGTAGAAGCATGCA+TGG | 0.580042 | 1.3:-2843110 | MS.gene26958:CDS |
| TCACAACAACAAGAAGAAGA+AGG | 0.587622 | 1.3:-2843166 | MS.gene26958:CDS |
| ATGCATGGTTAGAAAAGAAG+AGG | 0.601267 | 1.3:-2843095 | MS.gene26958:CDS |
| TGCATGGTTAGAAAAGAAGA+GGG | 0.637344 | 1.3:-2843094 | MS.gene26958:CDS |
| ATCTTCACAACAATCTTACG+AGG | 0.649549 | 1.3:+2843214 | None:intergenic |
| ATGGTTAGAAAAGAAGAGGG+AGG | 0.656282 | 1.3:-2843091 | MS.gene26958:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAAACAAAGGAAATTT+GGG | - | chr1.3:2843008-2843027 | MS.gene26958:CDS | 20.0% |
| ! | CACAAAAAACAAAGGAAATT+TGG | - | chr1.3:2843007-2843026 | MS.gene26958:CDS | 25.0% |
| ! | TAAGAAACCACAAAAAACAA+AGG | - | chr1.3:2842999-2843018 | MS.gene26958:CDS | 25.0% |
| ! | TATTGAGTACTACTCTTTAT+GGG | + | chr1.3:2843327-2843346 | None:intergenic | 25.0% |
| ! | TTATTGAGTACTACTCTTTA+TGG | + | chr1.3:2843328-2843347 | None:intergenic | 25.0% |
| !!! | CAAATTTCCTTTGTTTTTTG+TGG | + | chr1.3:2843009-2843028 | None:intergenic | 25.0% |
| ATCTTCACAACAATCTTACG+AGG | + | chr1.3:2843108-2843127 | None:intergenic | 35.0% | |
| ATGCATGGTTAGAAAAGAAG+AGG | - | chr1.3:2843224-2843243 | MS.gene26958:CDS | 35.0% | |
| GGTTCTATGAAGAAAGAAGA+AGG | - | chr1.3:2843174-2843193 | MS.gene26958:CDS | 35.0% | |
| TCACAACAACAAGAAGAAGA+AGG | - | chr1.3:2843153-2843172 | MS.gene26958:CDS | 35.0% | |
| TCGTAAGATTGTTGTGAAGA+TGG | - | chr1.3:2843107-2843126 | MS.gene26958:CDS | 35.0% | |
| TGCATGGTTAGAAAAGAAGA+GGG | - | chr1.3:2843225-2843244 | MS.gene26958:CDS | 35.0% | |
| ! | GAGATTCAACTTGTTCCAAA+AGG | - | chr1.3:2843255-2843274 | MS.gene26958:CDS | 35.0% |
| ATGGTTAGAAAAGAAGAGGG+AGG | - | chr1.3:2843228-2843247 | MS.gene26958:CDS | 40.0% | |
| ! | ATCAGAAGTAGAAGCATGCA+TGG | - | chr1.3:2843209-2843228 | MS.gene26958:CDS | 40.0% |
| CCTCAACCTACCAGAAAAAG+AGG | - | chr1.3:2843078-2843097 | MS.gene26958:CDS | 45.0% | |
| ! | ACGAGGTCTACCTCTTTTTC+TGG | + | chr1.3:2843091-2843110 | None:intergenic | 45.0% |
| ! | CAAGACACAGACACACCTTT+TGG | + | chr1.3:2843273-2843292 | None:intergenic | 45.0% |
| !! | CCTCTTTTTCTGGTAGGTTG+AGG | + | chr1.3:2843081-2843100 | None:intergenic | 45.0% |
| !! | GGTCTACCTCTTTTTCTGGT+AGG | + | chr1.3:2843087-2843106 | None:intergenic | 45.0% |
| CTTGTAGAAGCAGCAGAGCT+AGG | - | chr1.3:2843289-2843308 | MS.gene26958:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 2842994 | 2843347 | 2842994 | ID=MS.gene26958 |
| chr1.3 | mRNA | 2842994 | 2843347 | 2842994 | ID=MS.gene26958.t1;Parent=MS.gene26958 |
| chr1.3 | exon | 2842994 | 2843347 | 2842994 | ID=MS.gene26958.t1.exon1;Parent=MS.gene26958.t1 |
| chr1.3 | CDS | 2842994 | 2843347 | 2842994 | ID=cds.MS.gene26958.t1;Parent=MS.gene26958.t1 |
| Gene Sequence |
| Protein sequence |
