AlfalfaGEDB | MS.gene27822

M. sativa cultivar XinJiangDaYe / MS.gene27822

KEGG: -
GO: -
PFAM: PF02620.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27822.t1	XP_003629796.1	98.5	132	2	0	1	132	186	317	8.30E-68	266.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27822.t1	Q9LT27	58.1	129	50	3	3	131	190	314	2.6e-33	142.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27822.t1	G7LJG7	98.5	132	2	0	1	132	186	317	6.0e-68	266.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049758	MS.gene27822	0.8767	1.13E-68	-1.69E-46
MS.gene049910	MS.gene27822	0.816083	6.64E-52	-1.69E-46
MS.gene049915	MS.gene27822	0.80991	1.50E-50	-1.69E-46
MS.gene050099	MS.gene27822	0.804327	2.28E-49	-1.69E-46
MS.gene050301	MS.gene27822	0.841238	5.36E-58	-1.69E-46
MS.gene050397	MS.gene27822	0.815526	8.84E-52	-1.69E-46
MS.gene050464	MS.gene27822	0.818797	1.62E-52	-1.69E-46
MS.gene050529	MS.gene27822	0.850308	1.85E-60	-1.69E-46
MS.gene050558	MS.gene27822	0.815715	8.02E-52	-1.69E-46
MS.gene050642	MS.gene27822	0.824111	9.63E-54	-1.69E-46
MS.gene05101	MS.gene27822	0.813981	1.94E-51	-1.69E-46
MS.gene051471	MS.gene27822	0.806877	6.64E-50	-1.69E-46
MS.gene051662	MS.gene27822	0.8039	2.80E-49	-1.69E-46
MS.gene051663	MS.gene27822	0.83333	5.68E-56	-1.69E-46
MS.gene051860	MS.gene27822	0.848261	6.86E-60	-1.69E-46
MS.gene051983	MS.gene27822	0.817721	2.85E-52	-1.69E-46
MS.gene052077	MS.gene27822	0.82416	9.38E-54	-1.69E-46
MS.gene052078	MS.gene27822	0.808043	3.76E-50	-1.69E-46
MS.gene052178	MS.gene27822	0.823661	1.23E-53	-1.69E-46
MS.gene052188	MS.gene27822	0.828885	7.01E-55	-1.69E-46
MS.gene052253	MS.gene27822	0.806072	9.82E-50	-1.69E-46
MS.gene052310	MS.gene27822	0.817352	3.45E-52	-1.69E-46
MS.gene052340	MS.gene27822	0.828472	8.82E-55	-1.69E-46
MS.gene053557	MS.gene27822	0.826946	2.05E-54	-1.69E-46
MS.gene053836	MS.gene27822	0.877204	7.58E-69	-1.69E-46
MS.gene053907	MS.gene27822	0.801172	1.02E-48	-1.69E-46
MS.gene054930	MS.gene27822	0.811367	7.25E-51	-1.69E-46
MS.gene054983	MS.gene27822	0.825338	4.95E-54	-1.69E-46
MS.gene055116	MS.gene27822	0.808616	2.84E-50	-1.69E-46
MS.gene055277	MS.gene27822	0.835236	1.89E-56	-1.69E-46
MS.gene055390	MS.gene27822	0.814506	1.49E-51	-1.69E-46
MS.gene055422	MS.gene27822	0.837562	4.83E-57	-1.69E-46
MS.gene055428	MS.gene27822	0.819483	1.13E-52	-1.69E-46
MS.gene056612	MS.gene27822	0.847481	1.13E-59	-1.69E-46
MS.gene056979	MS.gene27822	0.813426	2.57E-51	-1.69E-46
MS.gene057121	MS.gene27822	0.850752	1.39E-60	-1.69E-46
MS.gene057260	MS.gene27822	0.806508	7.95E-50	-1.69E-46
MS.gene057439	MS.gene27822	0.820819	5.61E-53	-1.69E-46
MS.gene057450	MS.gene27822	0.800407	1.46E-48	-1.69E-46
MS.gene058002	MS.gene27822	0.859425	4.17E-63	-1.69E-46
MS.gene05811	MS.gene27822	0.864922	8.58E-65	-1.69E-46
MS.gene058142	MS.gene27822	0.824165	9.36E-54	-1.69E-46
MS.gene058233	MS.gene27822	0.802788	4.75E-49	-1.69E-46
MS.gene058276	MS.gene27822	0.810153	1.33E-50	-1.69E-46
MS.gene058660	MS.gene27822	0.802891	4.52E-49	-1.69E-46
MS.gene058749	MS.gene27822	0.838161	3.39E-57	-1.69E-46
MS.gene058845	MS.gene27822	0.911819	3.95E-83	-1.69E-46
MS.gene05907	MS.gene27822	0.844875	5.77E-59	-1.69E-46
MS.gene059518	MS.gene27822	0.827885	1.22E-54	-1.69E-46
MS.gene059597	MS.gene27822	0.825347	4.93E-54	-1.69E-46
MS.gene059688	MS.gene27822	0.814041	1.89E-51	-1.69E-46
MS.gene059692	MS.gene27822	0.818253	2.16E-52	-1.69E-46
MS.gene059695	MS.gene27822	0.820184	7.84E-53	-1.69E-46
MS.gene05984	MS.gene27822	0.856146	3.92E-62	-1.69E-46
MS.gene059894	MS.gene27822	0.837516	4.96E-57	-1.69E-46
MS.gene059899	MS.gene27822	0.817496	3.20E-52	-1.69E-46
MS.gene06037	MS.gene27822	0.801645	8.17E-49	-1.69E-46
MS.gene060476	MS.gene27822	0.81902	1.45E-52	-1.69E-46
MS.gene061049	MS.gene27822	0.855488	6.10E-62	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27822.t1	MTR_8g086630	98.485	132	2	0	1	132	186	317	3.52e-91	266
MS.gene27822.t1	MTR_4g086490	100.000	92	0	0	1	92	162	253	1.43e-57	192

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27822.t1	AT3G19810	61.240	129	46	3	3	131	190	314	2.91e-43	144
MS.gene27822.t1	AT3G19810	61.240	129	46	3	3	131	190	314	2.91e-43	144

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGAATTCAGTATGTGATTC+TGG	0.271880	4.1:-13524323	MS.gene27822:CDS
ATGTCAATTTGTTTCTCTTC+AGG	0.284068	4.1:+13524386	None:intergenic
GTGGTTCTTCAGTGAGTAAA+AGG	0.321200	4.1:+13524537	None:intergenic
ATAGTCTCTGGTTCATTAAC+AGG	0.341261	4.1:+13524515	None:intergenic
TGAAGATGCACTAATTGATT+TGG	0.358713	4.1:-13524429	MS.gene27822:CDS
TTTGCTACAGCTGCAATTGC+CGG	0.362751	4.1:+13524259	None:intergenic
TGTTAATGAACCAGAGACTA+TGG	0.398499	4.1:-13524513	MS.gene27822:CDS
GAGGAAAGCTTTGGCCCACT+TGG	0.410018	4.1:-13524224	MS.gene27822:CDS
GAAGTAAAAGAGGAAAGCTT+TGG	0.436034	4.1:-13524233	MS.gene27822:CDS
GAACCAGAGACTATGGATTT+CGG	0.437471	4.1:-13524506	MS.gene27822:CDS
GAGAATATGCTCTCGGCAGA+TGG	0.505785	4.1:+13524569	None:intergenic
TAGCAAAGAGGAAGTAAAAG+AGG	0.510851	4.1:-13524243	MS.gene27822:CDS
ACACCGAAATCCATAGTCTC+TGG	0.511153	4.1:+13524503	None:intergenic
GAAGACAAAATTCCAACCCT+TGG	0.539761	4.1:-13524470	MS.gene27822:CDS
TGAATTCAGTATGTGATTCT+GGG	0.541883	4.1:-13524322	MS.gene27822:CDS
TCAATTTGTTTCTCTTCAGG+AGG	0.559183	4.1:+13524389	None:intergenic
TGGTTCTTCAGTGAGTAAAA+GGG	0.584264	4.1:+13524538	None:intergenic
TGTGGTCAAAACTTTAACAC+CGG	0.593260	4.1:-13524278	MS.gene27822:CDS
GAACTCAGAGAATATGCTCT+CGG	0.593331	4.1:+13524562	None:intergenic
GTCTCTGGTTCATTAACAGG+TGG	0.598843	4.1:+13524518	None:intergenic
GCTCTCTTAGATTTCCAAGT+GGG	0.608668	4.1:+13524210	None:intergenic
TGCTCTCTTAGATTTCCAAG+TGG	0.611043	4.1:+13524209	None:intergenic
GTATGTGATTCTGGGTGCAA+AGG	0.624787	4.1:-13524314	MS.gene27822:CDS
CAATTGCAGCTGTAGCAAAG+AGG	0.644995	4.1:-13524255	MS.gene27822:CDS
AAAGGTGCGTGCCTGAAATG+TGG	0.693871	4.1:-13524296	MS.gene27822:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTCAAAAAACATAAGAGACA+GGG	-	chr4.1:13524405-13524424	MS.gene27822:CDS	25.0%
!	TTTCAAAAAACATAAGAGAC+AGG	-	chr4.1:13524404-13524423	MS.gene27822:CDS	25.0%
	ATGAATTCAGTATGTGATTC+TGG	-	chr4.1:13524445-13524464	MS.gene27822:CDS	30.0%
	TGAAGATGCACTAATTGATT+TGG	-	chr4.1:13524339-13524358	MS.gene27822:CDS	30.0%
	TGAATTCAGTATGTGATTCT+GGG	-	chr4.1:13524446-13524465	MS.gene27822:CDS	30.0%
!	ATGTCAATTTGTTTCTCTTC+AGG	+	chr4.1:13524385-13524404	None:intergenic	30.0%
!!	TAAAGTTTTGACCACATTTC+AGG	+	chr4.1:13524486-13524505	None:intergenic	30.0%
!!!	ATGGATTTCGGTGTTATTTT+TGG	-	chr4.1:13524274-13524293	MS.gene27822:CDS	30.0%
!!!	TGGATTTCGGTGTTATTTTT+GGG	-	chr4.1:13524275-13524294	MS.gene27822:CDS	30.0%
	GAAGTAAAAGAGGAAAGCTT+TGG	-	chr4.1:13524535-13524554	MS.gene27822:CDS	35.0%
	TAGCAAAGAGGAAGTAAAAG+AGG	-	chr4.1:13524525-13524544	MS.gene27822:CDS	35.0%
	TGGTTCTTCAGTGAGTAAAA+GGG	+	chr4.1:13524233-13524252	None:intergenic	35.0%
	TGTGGTCAAAACTTTAACAC+CGG	-	chr4.1:13524490-13524509	MS.gene27822:CDS	35.0%
	TGTTAATGAACCAGAGACTA+TGG	-	chr4.1:13524255-13524274	MS.gene27822:CDS	35.0%
!	ATAGTCTCTGGTTCATTAAC+AGG	+	chr4.1:13524256-13524275	None:intergenic	35.0%
!	TCAATTTGTTTCTCTTCAGG+AGG	+	chr4.1:13524382-13524401	None:intergenic	35.0%
!!!	GGATTTCGGTGTTATTTTTG+GGG	-	chr4.1:13524276-13524295	MS.gene27822:CDS	35.0%
	ATTCCAACCCTTGGAAAAAG+TGG	-	chr4.1:13524307-13524326	MS.gene27822:CDS	40.0%
	GAACCAGAGACTATGGATTT+CGG	-	chr4.1:13524262-13524281	MS.gene27822:CDS	40.0%
	GAACTCAGAGAATATGCTCT+CGG	+	chr4.1:13524209-13524228	None:intergenic	40.0%
	GAAGACAAAATTCCAACCCT+TGG	-	chr4.1:13524298-13524317	MS.gene27822:CDS	40.0%
	GCTCTCTTAGATTTCCAAGT+GGG	+	chr4.1:13524561-13524580	None:intergenic	40.0%
	GTGGTTCTTCAGTGAGTAAA+AGG	+	chr4.1:13524234-13524253	None:intergenic	40.0%
	TGCTCTCTTAGATTTCCAAG+TGG	+	chr4.1:13524562-13524581	None:intergenic	40.0%
	ACACCGAAATCCATAGTCTC+TGG	+	chr4.1:13524268-13524287	None:intergenic	45.0%
	CAATTGCAGCTGTAGCAAAG+AGG	-	chr4.1:13524513-13524532	MS.gene27822:CDS	45.0%
	GTATGTGATTCTGGGTGCAA+AGG	-	chr4.1:13524454-13524473	MS.gene27822:CDS	45.0%
	TTTGCTACAGCTGCAATTGC+CGG	+	chr4.1:13524512-13524531	None:intergenic	45.0%
!	GTCTCTGGTTCATTAACAGG+TGG	+	chr4.1:13524253-13524272	None:intergenic	45.0%
!!!	GTCATCGCCACTTTTTCCAA+GGG	+	chr4.1:13524317-13524336	None:intergenic	45.0%
	GAGAATATGCTCTCGGCAGA+TGG	+	chr4.1:13524202-13524221	None:intergenic	50.0%
!	AAAGGTGCGTGCCTGAAATG+TGG	-	chr4.1:13524472-13524491	MS.gene27822:CDS	50.0%
!!!	CGTCATCGCCACTTTTTCCA+AGG	+	chr4.1:13524318-13524337	None:intergenic	50.0%
!!!	TCGCCACTTTTTCCAAGGGT+TGG	+	chr4.1:13524313-13524332	None:intergenic	50.0%
!	GAGGAAAGCTTTGGCCCACT+TGG	-	chr4.1:13524544-13524563	MS.gene27822:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	13524196	13524594	13524196	ID=MS.gene27822
chr4.1	mRNA	13524196	13524594	13524196	ID=MS.gene27822.t1;Parent=MS.gene27822
chr4.1	exon	13524196	13524594	13524196	ID=MS.gene27822.t1.exon1;Parent=MS.gene27822.t1
chr4.1	CDS	13524196	13524594	13524196	ID=cds.MS.gene27822.t1;Parent=MS.gene27822.t1

Gene Sequence

>MS.gene27822

ATGCCATCTGCCGAGAGCATATTCTCTGAGTTCTCCCTTTTACTCACTGAAGAACCACCTGTTAATGAACCAGAGACTATGGATTTCGGTGTTATTTTTGGGGAAGACAAAATTCCAACCCTTGGAAAAAGTGGCGATGACGATGAAGATGCACTAATTGATTTGGATGATCAATTATATTTTCCTCCTGAAGAGAAACAAATTGACATTTCAAAAAACATAAGAGACAGGGTGCATCTTGAGATTACAATGAATTCAGTATGTGATTCTGGGTGCAAAGGTGCGTGCCTGAAATGTGGTCAAAACTTTAACACCGGCAATTGCAGCTGTAGCAAAGAGGAAGTAAAAGAGGAAAGCTTTGGCCCACTTGGAAATCTAAGAGAGCAGATGCAATTGTAA

Protein sequence

>MS.gene27822.t1

MPSAESIFSEFSLLLTEEPPVNEPETMDFGVIFGEDKIPTLGKSGDDDEDALIDLDDQLYFPPEEKQIDISKNIRDRVHLEITMNSVCDSGCKGACLKCGQNFNTGNCSCSKEEVKEESFGPLGNLREQMQL