Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32747.t1 | XP_003603233.2 | 94.9 | 117 | 6 | 0 | 1 | 117 | 1 | 117 | 2.70E-57 | 231.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32747.t1 | G7J943 | 94.9 | 117 | 6 | 0 | 1 | 117 | 1 | 117 | 1.9e-57 | 231.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene32747 | 0.811151 | 8.08E-51 | -1.69E-46 |
| MS.gene049262 | MS.gene32747 | 0.802322 | 5.93E-49 | -1.69E-46 |
| MS.gene049404 | MS.gene32747 | 0.802811 | 4.70E-49 | -1.69E-46 |
| MS.gene049435 | MS.gene32747 | 0.817958 | 2.52E-52 | -1.69E-46 |
| MS.gene050026 | MS.gene32747 | 0.808169 | 3.53E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene32747 | 0.800977 | 1.12E-48 | -1.69E-46 |
| MS.gene050442 | MS.gene32747 | 0.800271 | 1.56E-48 | -1.69E-46 |
| MS.gene050611 | MS.gene32747 | 0.811079 | 8.38E-51 | -1.69E-46 |
| MS.gene050680 | MS.gene32747 | 0.825697 | 4.07E-54 | -1.69E-46 |
| MS.gene050750 | MS.gene32747 | 0.804234 | 2.38E-49 | -1.69E-46 |
| MS.gene051181 | MS.gene32747 | 0.82126 | 4.44E-53 | -1.69E-46 |
| MS.gene051198 | MS.gene32747 | 0.809065 | 2.27E-50 | -1.69E-46 |
| MS.gene052190 | MS.gene32747 | 0.821627 | 3.65E-53 | -1.69E-46 |
| MS.gene052208 | MS.gene32747 | 0.813167 | 2.94E-51 | -1.69E-46 |
| MS.gene052333 | MS.gene32747 | 0.813529 | 2.44E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene32747 | 0.81335 | 2.68E-51 | -1.69E-46 |
| MS.gene052867 | MS.gene32747 | 0.819176 | 1.33E-52 | -1.69E-46 |
| MS.gene053166 | MS.gene32747 | 0.844291 | 8.28E-59 | -1.69E-46 |
| MS.gene053974 | MS.gene32747 | 0.811379 | 7.21E-51 | -1.69E-46 |
| MS.gene053998 | MS.gene32747 | 0.801764 | 7.72E-49 | -1.69E-46 |
| MS.gene05414 | MS.gene32747 | 0.831653 | 1.48E-55 | -1.69E-46 |
| MS.gene05415 | MS.gene32747 | 0.84284 | 2.02E-58 | -1.69E-46 |
| MS.gene054236 | MS.gene32747 | 0.8145 | 1.49E-51 | -1.69E-46 |
| MS.gene054291 | MS.gene32747 | -0.803728 | 3.04E-49 | -1.69E-46 |
| MS.gene05435 | MS.gene32747 | 0.831408 | 1.70E-55 | -1.69E-46 |
| MS.gene054405 | MS.gene32747 | 0.800622 | 1.32E-48 | -1.69E-46 |
| MS.gene054559 | MS.gene32747 | 0.837117 | 6.28E-57 | -1.69E-46 |
| MS.gene054651 | MS.gene32747 | 0.814805 | 1.28E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene32747 | 0.815026 | 1.14E-51 | -1.69E-46 |
| MS.gene055227 | MS.gene32747 | 0.809709 | 1.65E-50 | -1.69E-46 |
| MS.gene055228 | MS.gene32747 | 0.836413 | 9.49E-57 | -1.69E-46 |
| MS.gene055357 | MS.gene32747 | 0.808059 | 3.73E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene32747 | 0.805668 | 1.19E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene32747 | 0.826905 | 2.10E-54 | -1.69E-46 |
| MS.gene056162 | MS.gene32747 | 0.81118 | 7.96E-51 | -1.69E-46 |
| MS.gene056174 | MS.gene32747 | 0.803141 | 4.02E-49 | -1.69E-46 |
| MS.gene056252 | MS.gene32747 | 0.822182 | 2.72E-53 | -1.69E-46 |
| MS.gene057021 | MS.gene32747 | 0.813788 | 2.14E-51 | -1.69E-46 |
| MS.gene057060 | MS.gene32747 | 0.807701 | 4.44E-50 | -1.69E-46 |
| MS.gene057444 | MS.gene32747 | 0.810593 | 1.07E-50 | -1.69E-46 |
| MS.gene058010 | MS.gene32747 | 0.813919 | 2.01E-51 | -1.69E-46 |
| MS.gene058515 | MS.gene32747 | 0.838235 | 3.24E-57 | -1.69E-46 |
| MS.gene058979 | MS.gene32747 | 0.83397 | 3.93E-56 | -1.69E-46 |
| MS.gene059276 | MS.gene32747 | 0.812644 | 3.82E-51 | -1.69E-46 |
| MS.gene059550 | MS.gene32747 | 0.828919 | 6.88E-55 | -1.69E-46 |
| MS.gene059584 | MS.gene32747 | 0.829568 | 4.79E-55 | -1.69E-46 |
| MS.gene060011 | MS.gene32747 | 0.838631 | 2.56E-57 | -1.69E-46 |
| MS.gene060310 | MS.gene32747 | 0.801888 | 7.28E-49 | -1.69E-46 |
| MS.gene060843 | MS.gene32747 | 0.810386 | 1.18E-50 | -1.69E-46 |
| MS.gene060844 | MS.gene32747 | 0.809025 | 2.32E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32747.t1 | MTR_3g105340 | 94.068 | 118 | 7 | 0 | 1 | 118 | 1 | 118 | 1.73e-81 | 235 |
| MS.gene32747.t1 | MTR_1g014930 | 53.509 | 114 | 46 | 2 | 12 | 118 | 17 | 130 | 3.89e-36 | 120 |
| MS.gene32747.t1 | MTR_8g021190 | 55.208 | 96 | 41 | 1 | 8 | 101 | 27 | 122 | 3.64e-34 | 116 |
| MS.gene32747.t1 | MTR_4g125030 | 55.102 | 98 | 42 | 1 | 10 | 105 | 21 | 118 | 6.50e-32 | 109 |
| MS.gene32747.t1 | MTR_4g125010 | 46.957 | 115 | 55 | 3 | 5 | 115 | 18 | 130 | 6.51e-28 | 100 |
| MS.gene32747.t1 | MTR_5g006430 | 47.297 | 74 | 38 | 1 | 10 | 82 | 1 | 74 | 1.53e-22 | 85.1 |
| MS.gene32747.t1 | MTR_2g101140 | 52.778 | 72 | 33 | 1 | 1 | 72 | 52 | 122 | 1.06e-20 | 82.8 |
| MS.gene32747.t1 | MTR_8g013990 | 57.143 | 63 | 26 | 1 | 11 | 73 | 62 | 123 | 1.34e-20 | 82.4 |
| MS.gene32747.t1 | MTR_3g109860 | 36.111 | 108 | 68 | 1 | 11 | 117 | 36 | 143 | 8.85e-20 | 79.3 |
| MS.gene32747.t1 | MTR_1g103550 | 45.946 | 74 | 38 | 2 | 10 | 81 | 1 | 74 | 1.28e-19 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32747.t1 | AT1G72510 | 57.447 | 94 | 38 | 1 | 14 | 105 | 36 | 129 | 1.81e-34 | 117 |
| MS.gene32747.t1 | AT1G72510 | 57.447 | 94 | 38 | 1 | 14 | 105 | 36 | 129 | 1.81e-34 | 117 |
| MS.gene32747.t1 | AT2G09970 | 48.980 | 98 | 47 | 2 | 11 | 105 | 33 | 130 | 1.49e-27 | 99.8 |
| MS.gene32747.t1 | AT5G20670 | 48.454 | 97 | 45 | 3 | 12 | 105 | 23 | 117 | 7.62e-23 | 87.4 |
| MS.gene32747.t1 | AT1G54095 | 45.192 | 104 | 52 | 3 | 1 | 101 | 6 | 107 | 1.91e-21 | 83.6 |
| MS.gene32747.t1 | AT4G14819 | 36.697 | 109 | 62 | 3 | 10 | 118 | 1 | 102 | 3.47e-19 | 76.6 |
| MS.gene32747.t1 | AT3G22540 | 38.462 | 91 | 51 | 3 | 10 | 99 | 1 | 87 | 5.60e-18 | 73.6 |
| MS.gene32747.t1 | AT1G79770 | 43.878 | 98 | 47 | 3 | 7 | 100 | 47 | 140 | 9.80e-16 | 69.3 |
| MS.gene32747.t1 | AT5G25840 | 42.391 | 92 | 38 | 3 | 3 | 82 | 34 | 122 | 1.16e-13 | 63.9 |
| MS.gene32747.t1 | AT2G25780 | 38.202 | 89 | 51 | 3 | 15 | 100 | 39 | 126 | 1.44e-12 | 60.8 |
Find 30 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAATCAACCGTAGGTTTAT+TGG | 0.207844 | 3.1:+83178462 | None:intergenic |
| CTCCATCGTCGCCGCGGTTT+CGG | 0.297193 | 3.1:+83178696 | None:intergenic |
| AAATCAACCGTAGGTTTATT+GGG | 0.333585 | 3.1:+83178463 | None:intergenic |
| TACCTGGGTCGTTGGATTTG+TGG | 0.383506 | 3.1:-83178595 | MS.gene32747:CDS |
| ACCTGGGTCGTTGGATTTGT+GGG | 0.391134 | 3.1:-83178594 | MS.gene32747:CDS |
| GTGCTACTGTTGCGGCTTAA+CGG | 0.404880 | 3.1:-83178659 | MS.gene32747:CDS |
| CCATGTTCGTGAGAAATACC+TGG | 0.494919 | 3.1:-83178611 | MS.gene32747:CDS |
| GCCGCAACAGTAGCACTTCA+CGG | 0.509628 | 3.1:+83178666 | None:intergenic |
| GTTGGTAGATCGCATAGATA+AGG | 0.511238 | 3.1:-83178367 | None:intergenic |
| AAACTCAAAGGTTGTTCCCT+CGG | 0.525593 | 3.1:+83178397 | None:intergenic |
| GTGAGAAATACCTGGGTCGT+TGG | 0.543743 | 3.1:-83178603 | MS.gene32747:CDS |
| GTGTTGATTAAGAGCTTCAT+CGG | 0.550067 | 3.1:+83178516 | None:intergenic |
| TCGGTCTTTGGATTCTCCGA+GGG | 0.555608 | 3.1:-83178413 | MS.gene32747:CDS |
| AACCGCGGCGACGATGGAGG+TGG | 0.571492 | 3.1:-83178692 | MS.gene32747:CDS |
| TTCGGTCTTTGGATTCTCCG+AGG | 0.579754 | 3.1:-83178414 | MS.gene32747:CDS |
| AACCGAAACCGCGGCGACGA+TGG | 0.582114 | 3.1:-83178698 | MS.gene32747:CDS |
| CCAGGTATTTCTCACGAACA+TGG | 0.611108 | 3.1:+83178611 | None:intergenic |
| CGAAACCGCGGCGACGATGG+AGG | 0.615356 | 3.1:-83178695 | MS.gene32747:CDS |
| GATTTGTGGGCTATGTGCAG+AGG | 0.630857 | 3.1:-83178581 | MS.gene32747:CDS |
| GCCCACAAATCCAACGACCC+AGG | 0.634250 | 3.1:+83178593 | None:intergenic |
| AAGTCCTCCCAATAAACCTA+CGG | 0.635821 | 3.1:-83178470 | MS.gene32747:CDS |
| CCAACCGTTCGAAAACTCAA+AGG | 0.637318 | 3.1:+83178385 | None:intergenic |
| GCAAGAATCAAATCAACCGT+AGG | 0.641034 | 3.1:+83178454 | None:intergenic |
| CATGTTCGTGAGAAATACCT+GGG | 0.642264 | 3.1:-83178610 | MS.gene32747:CDS |
| GCCGTGAAGTGCTACTGTTG+CGG | 0.673482 | 3.1:-83178667 | MS.gene32747:CDS |
| CTACTGTTGCGGCTTAACGG+AGG | 0.677501 | 3.1:-83178656 | MS.gene32747:CDS |
| TACCACCTCCATCGTCGCCG+CGG | 0.680469 | 3.1:+83178690 | None:intergenic |
| TCAACCGTAGGTTTATTGGG+AGG | 0.689793 | 3.1:+83178466 | None:intergenic |
| GATGGCTTTAACCGAAACCG+CGG | 0.711918 | 3.1:-83178707 | MS.gene32747:CDS |
| CGAACATGGTCAATATACGA+CGG | 0.745897 | 3.1:+83178625 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATCAACCGTAGGTTTATT+GGG | + | chr3.1:83178615-83178634 | None:intergenic | 30.0% | |
| ! | CTGTGAAACATCTTCTTTTT+CGG | - | chr3.1:83178643-83178662 | MS.gene32747:CDS | 30.0% |
| CAAATCAACCGTAGGTTTAT+TGG | + | chr3.1:83178616-83178635 | None:intergenic | 35.0% | |
| !! | ACAACCTTTGAGTTTTCGAA+CGG | - | chr3.1:83178686-83178705 | MS.gene32747:CDS | 35.0% |
| !! | GTGTTGATTAAGAGCTTCAT+CGG | + | chr3.1:83178562-83178581 | None:intergenic | 35.0% |
| !!! | ACATCTTCTTTTTCGGTCTT+TGG | - | chr3.1:83178650-83178669 | MS.gene32747:CDS | 35.0% |
| AAACTCAAAGGTTGTTCCCT+CGG | + | chr3.1:83178681-83178700 | None:intergenic | 40.0% | |
| AAGTCCTCCCAATAAACCTA+CGG | - | chr3.1:83178605-83178624 | MS.gene32747:CDS | 40.0% | |
| CATGTTCGTGAGAAATACCT+GGG | - | chr3.1:83178465-83178484 | MS.gene32747:CDS | 40.0% | |
| CGAACATGGTCAATATACGA+CGG | + | chr3.1:83178453-83178472 | None:intergenic | 40.0% | |
| GCAAGAATCAAATCAACCGT+AGG | + | chr3.1:83178624-83178643 | None:intergenic | 40.0% | |
| CCAACCGTTCGAAAACTCAA+AGG | + | chr3.1:83178693-83178712 | None:intergenic | 45.0% | |
| CCAGGTATTTCTCACGAACA+TGG | + | chr3.1:83178467-83178486 | None:intergenic | 45.0% | |
| CCATGTTCGTGAGAAATACC+TGG | - | chr3.1:83178464-83178483 | MS.gene32747:CDS | 45.0% | |
| TCAACCGTAGGTTTATTGGG+AGG | + | chr3.1:83178612-83178631 | None:intergenic | 45.0% | |
| !! | CCTTTGAGTTTTCGAACGGT+TGG | - | chr3.1:83178690-83178709 | MS.gene32747:CDS | 45.0% |
| ACCTGGGTCGTTGGATTTGT+GGG | - | chr3.1:83178481-83178500 | MS.gene32747:CDS | 50.0% | |
| GTGAGAAATACCTGGGTCGT+TGG | - | chr3.1:83178472-83178491 | MS.gene32747:CDS | 50.0% | |
| TACCTGGGTCGTTGGATTTG+TGG | - | chr3.1:83178480-83178499 | MS.gene32747:CDS | 50.0% | |
| TCGGTCTTTGGATTCTCCGA+GGG | - | chr3.1:83178662-83178681 | MS.gene32747:CDS | 50.0% | |
| TTCGGTCTTTGGATTCTCCG+AGG | - | chr3.1:83178661-83178680 | MS.gene32747:CDS | 50.0% | |
| ! | GATTTGTGGGCTATGTGCAG+AGG | - | chr3.1:83178494-83178513 | MS.gene32747:CDS | 50.0% |
| ! | GTGCTACTGTTGCGGCTTAA+CGG | - | chr3.1:83178416-83178435 | MS.gene32747:CDS | 50.0% |
| CTACTGTTGCGGCTTAACGG+AGG | - | chr3.1:83178419-83178438 | MS.gene32747:CDS | 55.0% | |
| GCCGCAACAGTAGCACTTCA+CGG | + | chr3.1:83178412-83178431 | None:intergenic | 55.0% | |
| ! | GCCGTGAAGTGCTACTGTTG+CGG | - | chr3.1:83178408-83178427 | MS.gene32747:CDS | 55.0% |
| GCCCACAAATCCAACGACCC+AGG | + | chr3.1:83178485-83178504 | None:intergenic | 60.0% | |
| AACCGAAACCGCGGCGACGA+TGG | - | chr3.1:83178377-83178396 | MS.gene32747:CDS | 65.0% | |
| CTCCATCGTCGCCGCGGTTT+CGG | + | chr3.1:83178382-83178401 | None:intergenic | 65.0% | |
| TACCACCTCCATCGTCGCCG+CGG | + | chr3.1:83178388-83178407 | None:intergenic | 65.0% | |
| AACCGCGGCGACGATGGAGG+TGG | - | chr3.1:83178383-83178402 | MS.gene32747:CDS | 70.0% | |
| CGAAACCGCGGCGACGATGG+AGG | - | chr3.1:83178380-83178399 | MS.gene32747:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 83178369 | 83178728 | 83178369 | ID=MS.gene32747 |
| chr3.1 | mRNA | 83178369 | 83178728 | 83178369 | ID=MS.gene32747.t1;Parent=MS.gene32747 |
| chr3.1 | exon | 83178369 | 83178728 | 83178369 | ID=MS.gene32747.t1.exon1;Parent=MS.gene32747.t1 |
| chr3.1 | CDS | 83178369 | 83178728 | 83178369 | ID=cds.MS.gene32747.t1;Parent=MS.gene32747.t1 |
| Gene Sequence |
| Protein sequence |
