Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35592.t1 | XP_013460895.1 | 96.8 | 220 | 7 | 0 | 1 | 220 | 1 | 220 | 3.30E-117 | 431 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35592.t1 | A0A072UYT0 | 96.8 | 220 | 7 | 0 | 1 | 220 | 1 | 220 | 2.4e-117 | 431.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene35592 | 0.804362 | 2.24E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene35592 | 0.828758 | 7.52E-55 | -1.69E-46 |
| MS.gene050020 | MS.gene35592 | 0.807851 | 4.13E-50 | -1.69E-46 |
| MS.gene050128 | MS.gene35592 | 0.831981 | 1.23E-55 | -1.69E-46 |
| MS.gene050584 | MS.gene35592 | 0.832658 | 8.34E-56 | -1.69E-46 |
| MS.gene050585 | MS.gene35592 | 0.802352 | 5.84E-49 | -1.69E-46 |
| MS.gene050586 | MS.gene35592 | 0.829405 | 5.24E-55 | -1.69E-46 |
| MS.gene050762 | MS.gene35592 | 0.803851 | 2.86E-49 | -1.69E-46 |
| MS.gene050763 | MS.gene35592 | 0.805464 | 1.32E-49 | -1.69E-46 |
| MS.gene051626 | MS.gene35592 | 0.845249 | 4.57E-59 | -1.69E-46 |
| MS.gene051663 | MS.gene35592 | 0.806733 | 7.13E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene35592 | 0.893328 | 6.96E-75 | -1.69E-46 |
| MS.gene051860 | MS.gene35592 | 0.802644 | 5.09E-49 | -1.69E-46 |
| MS.gene052142 | MS.gene35592 | 0.806497 | 7.99E-50 | -1.69E-46 |
| MS.gene052178 | MS.gene35592 | 0.862537 | 4.72E-64 | -1.69E-46 |
| MS.gene052967 | MS.gene35592 | 0.832572 | 8.76E-56 | -1.69E-46 |
| MS.gene053074 | MS.gene35592 | 0.874923 | 4.62E-68 | -1.69E-46 |
| MS.gene053204 | MS.gene35592 | 0.812499 | 4.11E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene35592 | 0.823593 | 1.27E-53 | -1.69E-46 |
| MS.gene054011 | MS.gene35592 | 0.825694 | 4.08E-54 | -1.69E-46 |
| MS.gene054012 | MS.gene35592 | 0.817106 | 3.92E-52 | -1.69E-46 |
| MS.gene054013 | MS.gene35592 | 0.813213 | 2.87E-51 | -1.69E-46 |
| MS.gene054184 | MS.gene35592 | 0.828853 | 7.14E-55 | -1.69E-46 |
| MS.gene054185 | MS.gene35592 | 0.835111 | 2.03E-56 | -1.69E-46 |
| MS.gene054214 | MS.gene35592 | 0.868196 | 7.81E-66 | -1.69E-46 |
| MS.gene054379 | MS.gene35592 | 0.801734 | 7.83E-49 | -1.69E-46 |
| MS.gene05456 | MS.gene35592 | 0.842297 | 2.82E-58 | -1.69E-46 |
| MS.gene054596 | MS.gene35592 | 0.825431 | 4.71E-54 | -1.69E-46 |
| MS.gene055133 | MS.gene35592 | 0.81337 | 2.65E-51 | -1.69E-46 |
| MS.gene055207 | MS.gene35592 | 0.83277 | 7.82E-56 | -1.69E-46 |
| MS.gene055422 | MS.gene35592 | 0.80768 | 4.49E-50 | -1.69E-46 |
| MS.gene055428 | MS.gene35592 | 0.806556 | 7.77E-50 | -1.69E-46 |
| MS.gene055604 | MS.gene35592 | 0.80663 | 7.49E-50 | -1.69E-46 |
| MS.gene056069 | MS.gene35592 | -0.818235 | 2.18E-52 | -1.69E-46 |
| MS.gene057754 | MS.gene35592 | 0.813241 | 2.83E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene35592 | 0.828875 | 7.05E-55 | -1.69E-46 |
| MS.gene059006 | MS.gene35592 | 0.878156 | 3.53E-69 | -1.69E-46 |
| MS.gene059124 | MS.gene35592 | 0.812777 | 3.57E-51 | -1.69E-46 |
| MS.gene059228 | MS.gene35592 | 0.846731 | 1.81E-59 | -1.69E-46 |
| MS.gene06037 | MS.gene35592 | 0.818437 | 1.96E-52 | -1.69E-46 |
| MS.gene060476 | MS.gene35592 | 0.834157 | 3.52E-56 | -1.69E-46 |
| MS.gene061049 | MS.gene35592 | 0.816808 | 4.57E-52 | -1.69E-46 |
| MS.gene061112 | MS.gene35592 | 0.819238 | 1.29E-52 | -1.69E-46 |
| MS.gene061265 | MS.gene35592 | 0.818121 | 2.31E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35592.t1 | MTR_3g074450 | 96.818 | 220 | 7 | 0 | 1 | 220 | 1 | 220 | 1.07e-159 | 440 |
| MS.gene35592.t1 | MTR_5g063720 | 53.540 | 226 | 82 | 10 | 1 | 217 | 1 | 212 | 3.83e-58 | 183 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35592.t1 | AT1G78170 | 34.804 | 204 | 111 | 8 | 18 | 211 | 21 | 212 | 2.00e-19 | 83.6 |
| MS.gene35592.t1 | AT1G22250 | 40.870 | 115 | 55 | 4 | 101 | 208 | 80 | 188 | 4.83e-16 | 73.9 |
| MS.gene35592.t1 | AT4G08910 | 40.000 | 120 | 45 | 3 | 102 | 203 | 86 | 196 | 1.16e-15 | 73.2 |
Find 43 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATGTTTCCTTCAAATAAT+TGG | 0.175659 | 3.3:+64232637 | MS.gene35592:CDS |
| TTGAAGAATTCTTCAGTAAA+TGG | 0.216211 | 3.3:-64232555 | None:intergenic |
| GTGTCAATACCTTGGTATTC+AGG | 0.222403 | 3.3:+64232659 | MS.gene35592:CDS |
| TCTTCTTTAACTTCAGTTCT+TGG | 0.222864 | 3.3:-64232498 | None:intergenic |
| CATGGACAGTGCTTTGAATA+AGG | 0.336246 | 3.3:-64232472 | None:intergenic |
| CAGCTTCTTCTGCTCTAGTT+AGG | 0.340324 | 3.3:+64233200 | MS.gene35592:CDS |
| AGCAAGTTTAAGAAAGATTC+GGG | 0.373167 | 3.3:+64232994 | MS.gene35592:CDS |
| AAGCAAGTTTAAGAAAGATT+CGG | 0.375998 | 3.3:+64232993 | MS.gene35592:CDS |
| CATCTCTTTGTAATCTCCTT+CGG | 0.379098 | 3.3:-64233052 | None:intergenic |
| ATCATGTTCTGTCAAACTCT+TGG | 0.401354 | 3.3:-64232953 | None:intergenic |
| AAAGATTCGGGTGGTTTAAA+CGG | 0.409779 | 3.3:+64233006 | MS.gene35592:CDS |
| GTTTCTATCGTTGTCCGATC+GGG | 0.412127 | 3.3:-64232805 | None:intergenic |
| ATTTACTGAAGAATTCTTCA+AGG | 0.415230 | 3.3:+64232557 | MS.gene35592:CDS |
| ATCAAACAAATTTAGAGAAA+AGG | 0.427149 | 3.3:+64232580 | MS.gene35592:CDS |
| TGAAGGAAACATGGTCTCTA+GGG | 0.443563 | 3.3:-64232628 | None:intergenic |
| GGTTTCTATCGTTGTCCGAT+CGG | 0.443816 | 3.3:-64232806 | None:intergenic |
| TATTGACACCAATTATTTGA+AGG | 0.449231 | 3.3:-64232645 | None:intergenic |
| TTGTCGCAGGCTTCGTTTCG+AGG | 0.449883 | 3.3:-64232827 | None:intergenic |
| TCATGTTCTGTCAAACTCTT+GGG | 0.450709 | 3.3:-64232952 | None:intergenic |
| TTGAAGGAAACATGGTCTCT+AGG | 0.459950 | 3.3:-64232629 | None:intergenic |
| AGTGATCAGAAGAAGAATTC+TGG | 0.461088 | 3.3:+64232922 | MS.gene35592:CDS |
| AACTTCAGTTCTTGGAGTCA+TGG | 0.494815 | 3.3:-64232490 | None:intergenic |
| ATCAAAGAAAAGAAAGTTTG+AGG | 0.498556 | 3.3:+64232530 | MS.gene35592:CDS |
| GAGTGCATGAATTTGCAGTT+AGG | 0.515352 | 3.3:-64233138 | None:intergenic |
| AATAATTGGTGTCAATACCT+TGG | 0.524933 | 3.3:+64232651 | MS.gene35592:CDS |
| GTTTAAACGGTTCTCAGTCG+TGG | 0.546535 | 3.3:+64233019 | MS.gene35592:CDS |
| TAGGGGAGGCTTTGTATCAA+AGG | 0.546793 | 3.3:-64232610 | None:intergenic |
| AATTTGCAGTTAGGACAAGC+AGG | 0.557243 | 3.3:-64233129 | None:intergenic |
| CCAATTATTTGAAGGAAACA+TGG | 0.558834 | 3.3:-64232637 | None:intergenic |
| CGAAGGAGATTACAAAGAGA+TGG | 0.571998 | 3.3:+64233053 | MS.gene35592:CDS |
| CACATGTCAAGTTTAGCTCA+AGG | 0.577317 | 3.3:-64232876 | None:intergenic |
| TGCTTTGAATAAGGATACCA+TGG | 0.582273 | 3.3:-64232463 | None:intergenic |
| AAGCTCATGTGATCAGAAGA+AGG | 0.583375 | 3.3:-64232850 | None:intergenic |
| GGGAGGCTTTGTATCAAAGG+TGG | 0.591243 | 3.3:-64232607 | None:intergenic |
| GATCAGAAGAAGGTTGTCGC+AGG | 0.608082 | 3.3:-64232840 | None:intergenic |
| TCGTGGTTGTCGACAACCGA+AGG | 0.619983 | 3.3:+64233036 | MS.gene35592:CDS |
| TGTGTAACACAAGACCCGAT+CGG | 0.652579 | 3.3:+64232791 | MS.gene35592:CDS |
| ACTTGACATGTGAATCACAA+AGG | 0.656443 | 3.3:+64232887 | MS.gene35592:CDS |
| GGAAACATGGTCTCTAGGGG+AGG | 0.657415 | 3.3:-64232624 | None:intergenic |
| TTGCATGCAGTGTCACATGT+TGG | 0.659365 | 3.3:+64233086 | MS.gene35592:CDS |
| GAAGGAAACATGGTCTCTAG+GGG | 0.665977 | 3.3:-64232627 | None:intergenic |
| AAGTTTAAGAAAGATTCGGG+TGG | 0.705138 | 3.3:+64232997 | MS.gene35592:CDS |
| CAACATATACCTGAATACCA+AGG | 0.712025 | 3.3:-64232668 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAAACAAATTTAGAGAAA+AGG | + | chr3.3:64232580-64232599 | MS.gene35592:CDS | 20.0% |
| !! | ATCATAAGATATGATATCAT+TGG | - | chr3.3:64232729-64232748 | None:intergenic | 20.0% |
| ! | AAGCAAGTTTAAGAAAGATT+CGG | + | chr3.3:64232993-64233012 | MS.gene35592:CDS | 25.0% |
| ! | ATCAAAGAAAAGAAAGTTTG+AGG | + | chr3.3:64232530-64232549 | MS.gene35592:CDS | 25.0% |
| ! | ATTTACTGAAGAATTCTTCA+AGG | + | chr3.3:64232557-64232576 | MS.gene35592:CDS | 25.0% |
| ! | TATTGACACCAATTATTTGA+AGG | - | chr3.3:64232648-64232667 | None:intergenic | 25.0% |
| ! | TTGAAGAATTCTTCAGTAAA+TGG | - | chr3.3:64232558-64232577 | None:intergenic | 25.0% |
| !!! | AATTTTTTTTATGTGCAGTC+AGG | + | chr3.3:64232757-64232776 | MS.gene35592:intron | 25.0% |
| !!! | ATTTTTTTTATGTGCAGTCA+GGG | + | chr3.3:64232758-64232777 | MS.gene35592:intron | 25.0% |
| AGCAAGTTTAAGAAAGATTC+GGG | + | chr3.3:64232994-64233013 | MS.gene35592:CDS | 30.0% | |
| CCAATTATTTGAAGGAAACA+TGG | - | chr3.3:64232640-64232659 | None:intergenic | 30.0% | |
| CCATGTTTCCTTCAAATAAT+TGG | + | chr3.3:64232637-64232656 | MS.gene35592:CDS | 30.0% | |
| ! | TCTTCTTTAACTTCAGTTCT+TGG | - | chr3.3:64232501-64232520 | None:intergenic | 30.0% |
| ! | TGCTCTTCAAGAATTTTGAA+GGG | - | chr3.3:64233176-64233195 | None:intergenic | 30.0% |
| !! | AATAATTGGTGTCAATACCT+TGG | + | chr3.3:64232651-64232670 | MS.gene35592:CDS | 30.0% |
| AAAGATTCGGGTGGTTTAAA+CGG | + | chr3.3:64233006-64233025 | MS.gene35592:CDS | 35.0% | |
| AAGTTTAAGAAAGATTCGGG+TGG | + | chr3.3:64232997-64233016 | MS.gene35592:CDS | 35.0% | |
| ACTTGACATGTGAATCACAA+AGG | + | chr3.3:64232887-64232906 | MS.gene35592:CDS | 35.0% | |
| AGTGATCAGAAGAAGAATTC+TGG | + | chr3.3:64232922-64232941 | MS.gene35592:CDS | 35.0% | |
| ATCATGTTCTGTCAAACTCT+TGG | - | chr3.3:64232956-64232975 | None:intergenic | 35.0% | |
| CAACATATACCTGAATACCA+AGG | - | chr3.3:64232671-64232690 | None:intergenic | 35.0% | |
| CATCTCTTTGTAATCTCCTT+CGG | - | chr3.3:64233055-64233074 | None:intergenic | 35.0% | |
| TCATGTTCTGTCAAACTCTT+GGG | - | chr3.3:64232955-64232974 | None:intergenic | 35.0% | |
| ! | CTGCTCTTCAAGAATTTTGA+AGG | - | chr3.3:64233177-64233196 | None:intergenic | 35.0% |
| !! | TCAAGAATTTTGAAGGGTTC+TGG | - | chr3.3:64233170-64233189 | None:intergenic | 35.0% |
| AACTTCAGTTCTTGGAGTCA+TGG | - | chr3.3:64232493-64232512 | None:intergenic | 40.0% | |
| AAGCTCATGTGATCAGAAGA+AGG | - | chr3.3:64232853-64232872 | None:intergenic | 40.0% | |
| AATTTGCAGTTAGGACAAGC+AGG | - | chr3.3:64233132-64233151 | None:intergenic | 40.0% | |
| CGAAGGAGATTACAAAGAGA+TGG | + | chr3.3:64233053-64233072 | MS.gene35592:CDS | 40.0% | |
| GAGTGCATGAATTTGCAGTT+AGG | - | chr3.3:64233141-64233160 | None:intergenic | 40.0% | |
| GTGTCAATACCTTGGTATTC+AGG | + | chr3.3:64232659-64232678 | MS.gene35592:CDS | 40.0% | |
| TGAAGGAAACATGGTCTCTA+GGG | - | chr3.3:64232631-64232650 | None:intergenic | 40.0% | |
| TTGAAGGAAACATGGTCTCT+AGG | - | chr3.3:64232632-64232651 | None:intergenic | 40.0% | |
| ! | CACATGTCAAGTTTAGCTCA+AGG | - | chr3.3:64232879-64232898 | None:intergenic | 40.0% |
| ! | CATGGACAGTGCTTTGAATA+AGG | - | chr3.3:64232475-64232494 | None:intergenic | 40.0% |
| !! | AATTTTGAAGGGTTCTGGTC+TGG | - | chr3.3:64233165-64233184 | None:intergenic | 40.0% |
| GAAGGAAACATGGTCTCTAG+GGG | - | chr3.3:64232630-64232649 | None:intergenic | 45.0% | |
| GGTTTCTATCGTTGTCCGAT+CGG | - | chr3.3:64232809-64232828 | None:intergenic | 45.0% | |
| GTTTAAACGGTTCTCAGTCG+TGG | + | chr3.3:64233019-64233038 | MS.gene35592:CDS | 45.0% | |
| GTTTCTATCGTTGTCCGATC+GGG | - | chr3.3:64232808-64232827 | None:intergenic | 45.0% | |
| TGTGTAACACAAGACCCGAT+CGG | + | chr3.3:64232791-64232810 | MS.gene35592:CDS | 45.0% | |
| TTGCATGCAGTGTCACATGT+TGG | + | chr3.3:64233086-64233105 | MS.gene35592:CDS | 45.0% | |
| ! | TAGGGGAGGCTTTGTATCAA+AGG | - | chr3.3:64232613-64232632 | None:intergenic | 45.0% |
| GATCAGAAGAAGGTTGTCGC+AGG | - | chr3.3:64232843-64232862 | None:intergenic | 50.0% | |
| ! | GGGAGGCTTTGTATCAAAGG+TGG | - | chr3.3:64232610-64232629 | None:intergenic | 50.0% |
| TCGTGGTTGTCGACAACCGA+AGG | + | chr3.3:64233036-64233055 | MS.gene35592:CDS | 55.0% | |
| TTGTCGCAGGCTTCGTTTCG+AGG | - | chr3.3:64232830-64232849 | None:intergenic | 55.0% | |
| ! | GGAAACATGGTCTCTAGGGG+AGG | - | chr3.3:64232627-64232646 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 64232465 | 64233221 | 64232465 | ID=MS.gene35592 |
| chr3.3 | mRNA | 64232465 | 64233221 | 64232465 | ID=MS.gene35592.t1;Parent=MS.gene35592 |
| chr3.3 | exon | 64232465 | 64232680 | 64232465 | ID=MS.gene35592.t1.exon1;Parent=MS.gene35592.t1 |
| chr3.3 | CDS | 64232465 | 64232680 | 64232465 | ID=cds.MS.gene35592.t1;Parent=MS.gene35592.t1 |
| chr3.3 | exon | 64232775 | 64233221 | 64232775 | ID=MS.gene35592.t1.exon2;Parent=MS.gene35592.t1 |
| chr3.3 | CDS | 64232775 | 64233221 | 64232775 | ID=cds.MS.gene35592.t1;Parent=MS.gene35592.t1 |
| Gene Sequence |
| Protein sequence |
