Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37471.t1 | XP_024635917.1 | 96.2 | 158 | 5 | 1 | 1 | 157 | 1 | 158 | 2.20E-75 | 291.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37471.t1 | A0A072V3C0 | 96.2 | 158 | 5 | 1 | 1 | 157 | 1 | 158 | 1.6e-75 | 291.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04920 | MS.gene37471 | 0.804677 | 1.93E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene37471 | 0.869749 | 2.45E-66 | -1.69E-46 |
| MS.gene049499 | MS.gene37471 | 0.815382 | 9.51E-52 | -1.69E-46 |
| MS.gene049580 | MS.gene37471 | 0.801737 | 7.82E-49 | -1.69E-46 |
| MS.gene049680 | MS.gene37471 | 0.801452 | 8.95E-49 | -1.69E-46 |
| MS.gene04998 | MS.gene37471 | 0.806904 | 6.56E-50 | -1.69E-46 |
| MS.gene050414 | MS.gene37471 | 0.817252 | 3.63E-52 | -1.69E-46 |
| MS.gene050752 | MS.gene37471 | 0.808865 | 2.51E-50 | -1.69E-46 |
| MS.gene050945 | MS.gene37471 | 0.829864 | 4.05E-55 | -1.69E-46 |
| MS.gene051043 | MS.gene37471 | 0.835399 | 1.72E-56 | -1.69E-46 |
| MS.gene051699 | MS.gene37471 | 0.842056 | 3.27E-58 | -1.69E-46 |
| MS.gene051902 | MS.gene37471 | 0.811422 | 7.06E-51 | -1.69E-46 |
| MS.gene052296 | MS.gene37471 | 0.843701 | 1.19E-58 | -1.69E-46 |
| MS.gene052479 | MS.gene37471 | 0.809036 | 2.31E-50 | -1.69E-46 |
| MS.gene052793 | MS.gene37471 | 0.80041 | 1.46E-48 | -1.69E-46 |
| MS.gene052818 | MS.gene37471 | -0.832772 | 7.81E-56 | -1.69E-46 |
| MS.gene053298 | MS.gene37471 | 0.827719 | 1.34E-54 | -1.69E-46 |
| MS.gene053564 | MS.gene37471 | 0.805563 | 1.26E-49 | -1.69E-46 |
| MS.gene053879 | MS.gene37471 | 0.81819 | 2.23E-52 | -1.69E-46 |
| MS.gene053963 | MS.gene37471 | 0.846763 | 1.77E-59 | -1.69E-46 |
| MS.gene054222 | MS.gene37471 | 0.822322 | 2.52E-53 | -1.69E-46 |
| MS.gene054403 | MS.gene37471 | 0.814785 | 1.29E-51 | -1.69E-46 |
| MS.gene054413 | MS.gene37471 | 0.806131 | 9.55E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene37471 | 0.806421 | 8.30E-50 | -1.69E-46 |
| MS.gene054893 | MS.gene37471 | 0.814431 | 1.55E-51 | -1.69E-46 |
| MS.gene054957 | MS.gene37471 | 0.843688 | 1.20E-58 | -1.69E-46 |
| MS.gene055150 | MS.gene37471 | 0.801561 | 8.50E-49 | -1.69E-46 |
| MS.gene056100 | MS.gene37471 | 0.805578 | 1.25E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene37471 | 0.81279 | 3.55E-51 | -1.69E-46 |
| MS.gene057622 | MS.gene37471 | 0.838924 | 2.15E-57 | -1.69E-46 |
| MS.gene05781 | MS.gene37471 | 0.811738 | 6.03E-51 | -1.69E-46 |
| MS.gene057999 | MS.gene37471 | 0.840189 | 1.01E-57 | -1.69E-46 |
| MS.gene058074 | MS.gene37471 | 0.839843 | 1.24E-57 | -1.69E-46 |
| MS.gene058159 | MS.gene37471 | 0.84173 | 3.98E-58 | -1.69E-46 |
| MS.gene058279 | MS.gene37471 | 0.805626 | 1.22E-49 | -1.69E-46 |
| MS.gene058592 | MS.gene37471 | 0.811836 | 5.73E-51 | -1.69E-46 |
| MS.gene05878 | MS.gene37471 | 0.801891 | 7.27E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene37471 | 0.818438 | 1.96E-52 | -1.69E-46 |
| MS.gene05952 | MS.gene37471 | 0.853087 | 3.01E-61 | -1.69E-46 |
| MS.gene05985 | MS.gene37471 | -0.827307 | 1.68E-54 | -1.69E-46 |
| MS.gene059901 | MS.gene37471 | -0.807576 | 4.73E-50 | -1.69E-46 |
| MS.gene06018 | MS.gene37471 | 0.846862 | 1.66E-59 | -1.69E-46 |
| MS.gene060763 | MS.gene37471 | 0.829039 | 6.43E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene37471 | 0.813211 | 2.87E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37471.t1 | MTR_3g103290 | 96.203 | 158 | 5 | 1 | 1 | 157 | 1 | 158 | 2.77e-107 | 303 |
| MS.gene37471.t1 | MTR_1g017830 | 60.625 | 160 | 54 | 6 | 6 | 157 | 4 | 162 | 5.54e-49 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37471.t1 | AT1G10140 | 42.353 | 170 | 75 | 6 | 7 | 157 | 2 | 167 | 3.65e-23 | 89.7 |
| MS.gene37471.t1 | AT1G58420 | 37.931 | 174 | 76 | 6 | 8 | 153 | 6 | 175 | 7.67e-21 | 84.3 |
Find 40 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAGCATAGCTTCTACTTCT+TGG | 0.254156 | 3.4:+90930859 | None:intergenic |
| CATACCAACACTAACGGATT+TGG | 0.266128 | 3.4:+90930954 | None:intergenic |
| TTTGGGAAACCGAATTGATA+TGG | 0.303968 | 3.4:-90931031 | MS.gene37471:CDS |
| AAACCGAATTGATATGGATT+TGG | 0.312780 | 3.4:-90931025 | MS.gene37471:CDS |
| GCAGCTTCAGCTAGGACTTT+GGG | 0.347424 | 3.4:-90931048 | MS.gene37471:CDS |
| GGAAAAGCCTAGTGATGATA+TGG | 0.358613 | 3.4:-90930917 | MS.gene37471:CDS |
| AGCAGCTTCAGCTAGGACTT+TGG | 0.364411 | 3.4:-90931049 | MS.gene37471:CDS |
| GGATAAGAATTTGCCACAAC+AGG | 0.377510 | 3.4:+90930880 | None:intergenic |
| ATACCAACACTAACGGATTT+GGG | 0.389033 | 3.4:+90930955 | None:intergenic |
| CAAATCCGTTAGTGTTGGTA+TGG | 0.422959 | 3.4:-90930953 | MS.gene37471:CDS |
| GGGTAACCCGTTTGAAGAAA+CGG | 0.435006 | 3.4:+90930975 | None:intergenic |
| CAAAACCCGTTTCTTCAAAC+GGG | 0.435681 | 3.4:-90930981 | MS.gene37471:CDS |
| GAACCAAATCCATATCAATT+CGG | 0.436672 | 3.4:+90931022 | None:intergenic |
| GGTAACCCGTTTGAAGAAAC+GGG | 0.437522 | 3.4:+90930976 | None:intergenic |
| ACAAAACCCGTTTCTTCAAA+CGG | 0.444703 | 3.4:-90930982 | MS.gene37471:CDS |
| ACCTTGGAGATGAGGAAAAG+TGG | 0.460082 | 3.4:-90931294 | None:intergenic |
| ATGAATTACAGCAATCCTAC+TGG | 0.466774 | 3.4:-90931162 | MS.gene37471:CDS |
| ACAACACCAATAAGAGTGTT+GGG | 0.479331 | 3.4:-90931234 | MS.gene37471:CDS |
| AACAACACCAATAAGAGTGT+TGG | 0.503361 | 3.4:-90931235 | MS.gene37471:CDS |
| CTCATTATCACCACCACCAC+CGG | 0.515759 | 3.4:+90931092 | None:intergenic |
| CCGTTAGTGTTGGTATGGGT+CGG | 0.519449 | 3.4:-90930948 | MS.gene37471:CDS |
| ACTTCTCGATCCGGTGGTGG+TGG | 0.522423 | 3.4:-90931102 | MS.gene37471:CDS |
| AGCGCAGTAACTTCTCGATC+CGG | 0.538267 | 3.4:-90931111 | MS.gene37471:CDS |
| AGAAGTAGAAGCTATGCTGT+TGG | 0.550220 | 3.4:-90930856 | MS.gene37471:CDS |
| GTAACTTCTCGATCCGGTGG+TGG | 0.556180 | 3.4:-90931105 | MS.gene37471:CDS |
| TAATGAGAGCAGCTTCAGCT+AGG | 0.560584 | 3.4:-90931056 | MS.gene37471:CDS |
| CCTTCACCCATATCATCACT+AGG | 0.561979 | 3.4:+90930910 | None:intergenic |
| GTTACTGCGCTGTAACTTCG+CGG | 0.566026 | 3.4:+90931123 | None:intergenic |
| CGGTGGTGGTGGTGATAATG+AGG | 0.566967 | 3.4:-90931091 | MS.gene37471:CDS |
| CCTTGGAGATGAGGAAAAGT+GGG | 0.569313 | 3.4:-90931293 | None:intergenic |
| TGAAGGTGATGTTCCTGTTG+TGG | 0.576444 | 3.4:-90930893 | MS.gene37471:CDS |
| TTACCCAAATCCGTTAGTGT+TGG | 0.593589 | 3.4:-90930958 | MS.gene37471:CDS |
| CCGACCCATACCAACACTAA+CGG | 0.605120 | 3.4:+90930948 | None:intergenic |
| AAATCCGTTAGTGTTGGTAT+GGG | 0.605156 | 3.4:-90930952 | MS.gene37471:CDS |
| AAATTCTCGAATCTTCCAGT+AGG | 0.622390 | 3.4:+90931147 | None:intergenic |
| GAAAAGCCTAGTGATGATAT+GGG | 0.622804 | 3.4:-90930916 | MS.gene37471:CDS |
| GCAGTAACTTCTCGATCCGG+TGG | 0.647445 | 3.4:-90931108 | MS.gene37471:CDS |
| TGGTATGGGTCGGATTGATG+AGG | 0.659406 | 3.4:-90930938 | MS.gene37471:CDS |
| CCTAGTGATGATATGGGTGA+AGG | 0.664408 | 3.4:-90930910 | MS.gene37471:CDS |
| GTAAGAAGCATAACAAAGTG+CGG | 0.704624 | 3.4:-90931192 | MS.gene37471:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACCGAATTGATATGGATT+TGG | - | chr3.4:90931094-90931113 | MS.gene37471:CDS | 30.0% | |
| GAACCAAATCCATATCAATT+CGG | + | chr3.4:90931100-90931119 | None:intergenic | 30.0% | |
| AAATTCTCGAATCTTCCAGT+AGG | + | chr3.4:90930975-90930994 | None:intergenic | 35.0% | |
| ACAAAACCCGTTTCTTCAAA+CGG | - | chr3.4:90931137-90931156 | MS.gene37471:CDS | 35.0% | |
| ATGAATTACAGCAATCCTAC+TGG | - | chr3.4:90930957-90930976 | MS.gene37471:CDS | 35.0% | |
| GAAAAGCCTAGTGATGATAT+GGG | - | chr3.4:90931203-90931222 | MS.gene37471:CDS | 35.0% | |
| TTTGGGAAACCGAATTGATA+TGG | - | chr3.4:90931088-90931107 | MS.gene37471:CDS | 35.0% | |
| ! | AACAACACCAATAAGAGTGT+TGG | - | chr3.4:90930884-90930903 | MS.gene37471:CDS | 35.0% |
| ! | GTAAGAAGCATAACAAAGTG+CGG | - | chr3.4:90930927-90930946 | MS.gene37471:CDS | 35.0% |
| !! | AAATCCGTTAGTGTTGGTAT+GGG | - | chr3.4:90931167-90931186 | MS.gene37471:CDS | 35.0% |
| !! | ACAACACCAATAAGAGTGTT+GGG | - | chr3.4:90930885-90930904 | MS.gene37471:CDS | 35.0% |
| !! | ATACCAACACTAACGGATTT+GGG | + | chr3.4:90931167-90931186 | None:intergenic | 35.0% |
| ACAGCATAGCTTCTACTTCT+TGG | + | chr3.4:90931263-90931282 | None:intergenic | 40.0% | |
| AGAAGTAGAAGCTATGCTGT+TGG | - | chr3.4:90931263-90931282 | MS.gene37471:CDS | 40.0% | |
| CAAAACCCGTTTCTTCAAAC+GGG | - | chr3.4:90931138-90931157 | MS.gene37471:CDS | 40.0% | |
| GGAAAAGCCTAGTGATGATA+TGG | - | chr3.4:90931202-90931221 | MS.gene37471:CDS | 40.0% | |
| GGATAAGAATTTGCCACAAC+AGG | + | chr3.4:90931242-90931261 | None:intergenic | 40.0% | |
| TTACCCAAATCCGTTAGTGT+TGG | - | chr3.4:90931161-90931180 | MS.gene37471:CDS | 40.0% | |
| ! | GCTTTTCCCAACACTCTTAT+TGG | + | chr3.4:90930894-90930913 | None:intergenic | 40.0% |
| !! | CAAATCCGTTAGTGTTGGTA+TGG | - | chr3.4:90931166-90931185 | MS.gene37471:CDS | 40.0% |
| !! | CATACCAACACTAACGGATT+TGG | + | chr3.4:90931168-90931187 | None:intergenic | 40.0% |
| CCTAGTGATGATATGGGTGA+AGG | - | chr3.4:90931209-90931228 | MS.gene37471:CDS | 45.0% | |
| CCTTCACCCATATCATCACT+AGG | + | chr3.4:90931212-90931231 | None:intergenic | 45.0% | |
| GGGTAACCCGTTTGAAGAAA+CGG | + | chr3.4:90931147-90931166 | None:intergenic | 45.0% | |
| GGTAACCCGTTTGAAGAAAC+GGG | + | chr3.4:90931146-90931165 | None:intergenic | 45.0% | |
| TAATGAGAGCAGCTTCAGCT+AGG | - | chr3.4:90931063-90931082 | MS.gene37471:CDS | 45.0% | |
| TGAAGGTGATGTTCCTGTTG+TGG | - | chr3.4:90931226-90931245 | MS.gene37471:CDS | 45.0% | |
| AGCAGCTTCAGCTAGGACTT+TGG | - | chr3.4:90931070-90931089 | MS.gene37471:CDS | 50.0% | |
| AGCGCAGTAACTTCTCGATC+CGG | - | chr3.4:90931008-90931027 | MS.gene37471:CDS | 50.0% | |
| CCGACCCATACCAACACTAA+CGG | + | chr3.4:90931174-90931193 | None:intergenic | 50.0% | |
| CTCATTATCACCACCACCAC+CGG | + | chr3.4:90931030-90931049 | None:intergenic | 50.0% | |
| GCAGCTTCAGCTAGGACTTT+GGG | - | chr3.4:90931071-90931090 | MS.gene37471:CDS | 50.0% | |
| GTTACTGCGCTGTAACTTCG+CGG | + | chr3.4:90930999-90931018 | None:intergenic | 50.0% | |
| TGGTATGGGTCGGATTGATG+AGG | - | chr3.4:90931181-90931200 | MS.gene37471:CDS | 50.0% | |
| !! | CCGTTAGTGTTGGTATGGGT+CGG | - | chr3.4:90931171-90931190 | MS.gene37471:CDS | 50.0% |
| GCAGTAACTTCTCGATCCGG+TGG | - | chr3.4:90931011-90931030 | MS.gene37471:CDS | 55.0% | |
| GTAACTTCTCGATCCGGTGG+TGG | - | chr3.4:90931014-90931033 | MS.gene37471:CDS | 55.0% | |
| !! | CGGTGGTGGTGGTGATAATG+AGG | - | chr3.4:90931028-90931047 | MS.gene37471:CDS | 55.0% |
| ! | ACTTCTCGATCCGGTGGTGG+TGG | - | chr3.4:90931017-90931036 | MS.gene37471:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 90930834 | 90931307 | 90930834 | ID=MS.gene37471 |
| chr3.4 | mRNA | 90930834 | 90931307 | 90930834 | ID=MS.gene37471.t1;Parent=MS.gene37471 |
| chr3.4 | exon | 90930834 | 90931307 | 90930834 | ID=MS.gene37471.t1.exon1;Parent=MS.gene37471.t1 |
| chr3.4 | CDS | 90930834 | 90931307 | 90930834 | ID=cds.MS.gene37471.t1;Parent=MS.gene37471.t1 |
| Gene Sequence |
| Protein sequence |
