AlfalfaGEDB | MS.gene37513

M. sativa cultivar XinJiangDaYe / MS.gene37513

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0008150 GO:0008152 GO:0016491 GO:0016614 GO:0016616 GO:0036094 GO:0048037 GO:0050662 GO:0051287 GO:0055114 GO:0097159 GO:1901265 GO:1901363
PFAM: PF00389.33

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene37513.t1	KEH19019.1	96.9	161	5	0	1	161	1	161	5.50E-83	317

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene37513.t1	Q9LE33	43.7	151	79	3	13	161	3	149	1.0e-21	104.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene37513.t1	A0A072TNE4	96.9	161	5	0	1	161	1	161	4.0e-83	317.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene87846	MS.gene37513	PPI
MS.gene23624	MS.gene37513	PPI
MS.gene24945	MS.gene37513	PPI
MS.gene37513	MS.gene33396	PPI
MS.gene019406	MS.gene37513	PPI
MS.gene24946	MS.gene37513	PPI
MS.gene06894	MS.gene37513	PPI
MS.gene87848	MS.gene37513	PPI
MS.gene068259	MS.gene37513	PPI
MS.gene23633	MS.gene37513	PPI
MS.gene37513	MS.gene37515	PPI
MS.gene87842	MS.gene37513	PPI
MS.gene37508	MS.gene37513	PPI
MS.gene06683	MS.gene37513	PPI
MS.gene057128	MS.gene37513	PPI
MS.gene37513	MS.gene019402	PPI
MS.gene37513	MS.gene068262	PPI
MS.gene068263	MS.gene37513	PPI
MS.gene37513	MS.gene33394	PPI
MS.gene37513	MS.gene019404	PPI
MS.gene33395	MS.gene37513	PPI
MS.gene013566	MS.gene37513	PPI
MS.gene24947	MS.gene37513	PPI
MS.gene37513	MS.gene37512	PPI
MS.gene23623	MS.gene37513	PPI
MS.gene019405	MS.gene37513	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene37513.t1	MTR_8g033400	96.894	161	5	0	1	161	1	161	2.50e-113	325
MS.gene37513.t1	MTR_8g033410	70.199	151	44	1	12	161	4	154	1.84e-71	219
MS.gene37513.t1	MTR_8g033390	53.691	149	67	2	15	161	3	151	1.05e-46	155
MS.gene37513.t1	MTR_7g075830	48.819	127	57	4	51	175	19	139	2.77e-25	99.4
MS.gene37513.t1	MTR_4g012960	30.769	130	83	2	32	161	16	138	6.92e-18	79.3
MS.gene37513.t1	MTR_4g013090	31.452	124	78	2	32	155	16	132	8.64e-17	76.6
MS.gene37513.t1	MTR_4g013100	29.032	124	81	2	32	155	17	133	1.02e-15	73.6
MS.gene37513.t1	MTR_2g101840	35.338	133	76	3	32	161	16	141	1.74e-13	67.4
MS.gene37513.t1	MTR_4g013135	31.034	116	73	2	40	155	24	132	4.32e-12	63.5
MS.gene37513.t1	MTR_4g013040	36.000	75	44	1	81	155	1	71	5.05e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene37513.t1	AT2G45630	45.783	166	78	4	5	161	3	165	2.98e-38	129
MS.gene37513.t1	AT2G45630	45.783	166	78	4	5	161	3	165	2.38e-36	129
MS.gene37513.t1	AT1G12550	41.176	170	91	4	13	177	3	168	7.11e-29	109
MS.gene37513.t1	AT1G79870	34.615	104	61	2	61	161	42	141	2.87e-11	61.2
MS.gene37513.t1	AT1G79870	34.737	95	58	1	61	155	42	132	2.89e-11	60.8

Find 53 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAATTTCCACTTGGTTCTA+AGG	0.218406	8.3:+60319917	MS.gene37513:CDS
CATAATATGTCTGTTTAATA+TGG	0.231956	8.3:+60319978	MS.gene37513:CDS
TTCTGAAGATGTTGCTGATA+TGG	0.254694	8.3:+60319812	MS.gene37513:CDS
TTGGAGGATGAAGGAGAATT+CGG	0.310098	8.3:-60319532	None:intergenic
GGTCCATGAAGGAATACTTT+TGG	0.327666	8.3:-60319507	None:intergenic
GTGTCAGCGTCGGGGAATTC+AGG	0.351701	8.3:+60319767	MS.gene37513:CDS
CTCCCAAAAGTATTCCTTCA+TGG	0.370851	8.3:+60319504	MS.gene37513:CDS
TGGAAATTGTGAGAAGGATT+TGG	0.375802	8.3:-60319555	None:intergenic
TGGAGGATGAAGGAGAATTC+GGG	0.378466	8.3:-60319531	None:intergenic
GAGAGGAAGGGTTTAAGATA+TGG	0.380318	8.3:-60319575	None:intergenic
TGGAGAGGAGGAAGAGAGGA+AGG	0.396752	8.3:-60319588	None:intergenic
GGAATTCAGGTTGCCGGAGC+TGG	0.396906	8.3:+60319780	MS.gene37513:CDS
AGGATTTGGTTGGAGGATGA+AGG	0.397061	8.3:-60319541	None:intergenic
TCTCTCTGAGTGTCAGCGTC+GGG	0.401832	8.3:+60319758	MS.gene37513:CDS
GGTGCAGACAAGACGGAGGG+CGG	0.407749	8.3:-60319709	None:intergenic
GGAGGCATGGTGAGGATTGA+GGG	0.414126	8.3:-60319622	None:intergenic
TGTGAGAAGGATTTGGTTGG+AGG	0.436630	8.3:-60319548	None:intergenic
CGTCGGGGAATTCAGGTTGC+CGG	0.442476	8.3:+60319774	MS.gene37513:CDS
GAGATCAATATGATCAGTTC+CGG	0.442837	8.3:-60319739	None:intergenic
AGGAGGCATGGTGAGGATTG+AGG	0.447068	8.3:-60319623	None:intergenic
TTGGTGGAGAGGAGGAAGAG+AGG	0.453807	8.3:-60319592	None:intergenic
CTTTGTTGATTGATGTCAGC+AGG	0.466747	8.3:+60319841	MS.gene37513:CDS
GTGCAGACAAGACGGAGGGC+GGG	0.469363	8.3:-60319708	None:intergenic
TTGGAGAACATCAGCGTTGA+CGG	0.477559	8.3:-60319679	None:intergenic
ATATGATCAGTTCCGGCGCT+GGG	0.489486	8.3:-60319732	None:intergenic
GACGGAGGGCGGGAAGAAGT+TGG	0.491857	8.3:-60319698	None:intergenic
ATCTCTCTGAGTGTCAGCGT+CGG	0.494720	8.3:+60319757	MS.gene37513:CDS
AATATGATCAGTTCCGGCGC+TGG	0.498476	8.3:-60319733	None:intergenic
GACGATAGCGGCAATGGAGG+AGG	0.501959	8.3:-60319640	None:intergenic
GAGGATTGAGGGATAAGAAT+TGG	0.512540	8.3:-60319611	None:intergenic
GATAAGAATTGGTGGAGAGG+AGG	0.525383	8.3:-60319600	None:intergenic
AATTGTGAGAAGGATTTGGT+TGG	0.530046	8.3:-60319551	None:intergenic
ATGATCAGTTCCGGCGCTGG+GGG	0.534589	8.3:-60319730	None:intergenic
GGTACAGACGATAGCGGCAA+TGG	0.548937	8.3:-60319646	None:intergenic
GGAGAGGAGGAAGAGAGGAA+GGG	0.552439	8.3:-60319587	None:intergenic
AGCGTTGACGGTGTAGCCTC+CGG	0.553059	8.3:-60319667	None:intergenic
GGAAAATTTCAGCTGCAGAT+CGG	0.554245	8.3:+60319862	MS.gene37513:CDS
GACAAGTTCAGAATTCAACT+TGG	0.556290	8.3:+60319895	MS.gene37513:CDS
AAGATATGGAAATTGTGAGA+AGG	0.559376	8.3:-60319561	None:intergenic
GATTGAGGGATAAGAATTGG+TGG	0.574137	8.3:-60319608	None:intergenic
TATGATCAGTTCCGGCGCTG+GGG	0.575534	8.3:-60319731	None:intergenic
AGGGATAAGAATTGGTGGAG+AGG	0.577295	8.3:-60319603	None:intergenic
AGAATTCGGGTGGTCCATGA+AGG	0.577437	8.3:-60319518	None:intergenic
TCAACTTGGAAATTTCCACT+TGG	0.589811	8.3:+60319909	MS.gene37513:CDS
CTCTCTGAGTGTCAGCGTCG+GGG	0.607669	8.3:+60319759	MS.gene37513:CDS
TAGCGGCAATGGAGGAGGCA+TGG	0.609190	8.3:-60319635	None:intergenic
ACAGACGATAGCGGCAATGG+AGG	0.613970	8.3:-60319643	None:intergenic
GCCGCTATCGTCTGTACCGG+AGG	0.617073	8.3:+60319651	MS.gene37513:CDS
TTGCATACCTTAGAACCAAG+TGG	0.628108	8.3:-60319924	None:intergenic
ATTGCCGCTATCGTCTGTAC+CGG	0.633546	8.3:+60319648	MS.gene37513:CDS
GCAATGGAGGAGGCATGGTG+AGG	0.666359	8.3:-60319630	None:intergenic
AGGATGAAGGAGAATTCGGG+TGG	0.719251	8.3:-60319528	None:intergenic
GCCTCCGGTACAGACGATAG+CGG	0.744766	8.3:-60319652	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATAATATGTCTGTTTAATA+TGG	+	chr8.3:60319978-60319997	MS.gene37513:CDS	20.0%
!!	TGGGAGATTATTATTTTCTT+GGG	-	chr8.3:60319490-60319509	None:intergenic	25.0%
!!	TTGGGAGATTATTATTTTCT+TGG	-	chr8.3:60319491-60319510	None:intergenic	25.0%
	AAGATATGGAAATTGTGAGA+AGG	-	chr8.3:60319564-60319583	None:intergenic	30.0%
	AGAAAAAAGATTTCCAGCTC+CGG	-	chr8.3:60319796-60319815	None:intergenic	35.0%
	GACAAGTTCAGAATTCAACT+TGG	+	chr8.3:60319895-60319914	MS.gene37513:CDS	35.0%
	GAGATCAATATGATCAGTTC+CGG	-	chr8.3:60319742-60319761	None:intergenic	35.0%
	TCAACTTGGAAATTTCCACT+TGG	+	chr8.3:60319909-60319928	MS.gene37513:CDS	35.0%
	TGGAAATTGTGAGAAGGATT+TGG	-	chr8.3:60319558-60319577	None:intergenic	35.0%
!	AATTGTGAGAAGGATTTGGT+TGG	-	chr8.3:60319554-60319573	None:intergenic	35.0%
!	GTCCATGAAGGAATACTTTT+GGG	-	chr8.3:60319509-60319528	None:intergenic	35.0%
!	TTCTGAAGATGTTGCTGATA+TGG	+	chr8.3:60319812-60319831	MS.gene37513:CDS	35.0%
!!	GAAATTTCCACTTGGTTCTA+AGG	+	chr8.3:60319917-60319936	MS.gene37513:CDS	35.0%
	GAGGATTGAGGGATAAGAAT+TGG	-	chr8.3:60319614-60319633	None:intergenic	40.0%
	GATTGAGGGATAAGAATTGG+TGG	-	chr8.3:60319611-60319630	None:intergenic	40.0%
	GGAAAATTTCAGCTGCAGAT+CGG	+	chr8.3:60319862-60319881	MS.gene37513:CDS	40.0%
	TTGCATACCTTAGAACCAAG+TGG	-	chr8.3:60319927-60319946	None:intergenic	40.0%
	TTGGAGGATGAAGGAGAATT+CGG	-	chr8.3:60319535-60319554	None:intergenic	40.0%
!	CTTTGTTGATTGATGTCAGC+AGG	+	chr8.3:60319841-60319860	MS.gene37513:CDS	40.0%
!	GAGAGGAAGGGTTTAAGATA+TGG	-	chr8.3:60319578-60319597	None:intergenic	40.0%
!	GGTCCATGAAGGAATACTTT+TGG	-	chr8.3:60319510-60319529	None:intergenic	40.0%
!!	CTCCCAAAAGTATTCCTTCA+TGG	+	chr8.3:60319504-60319523	MS.gene37513:CDS	40.0%
	AGGGATAAGAATTGGTGGAG+AGG	-	chr8.3:60319606-60319625	None:intergenic	45.0%
	GATAAGAATTGGTGGAGAGG+AGG	-	chr8.3:60319603-60319622	None:intergenic	45.0%
	TGGAGGATGAAGGAGAATTC+GGG	-	chr8.3:60319534-60319553	None:intergenic	45.0%
!	AGGATTTGGTTGGAGGATGA+AGG	-	chr8.3:60319544-60319563	None:intergenic	45.0%
!	TGTGAGAAGGATTTGGTTGG+AGG	-	chr8.3:60319551-60319570	None:intergenic	45.0%
!	TTGGAGAACATCAGCGTTGA+CGG	-	chr8.3:60319682-60319701	None:intergenic	45.0%
	AATATGATCAGTTCCGGCGC+TGG	-	chr8.3:60319736-60319755	None:intergenic	50.0%
	AGAATTCGGGTGGTCCATGA+AGG	-	chr8.3:60319521-60319540	None:intergenic	50.0%
	AGGATGAAGGAGAATTCGGG+TGG	-	chr8.3:60319531-60319550	None:intergenic	50.0%
	ATATGATCAGTTCCGGCGCT+GGG	-	chr8.3:60319735-60319754	None:intergenic	50.0%
	ATCTCTCTGAGTGTCAGCGT+CGG	+	chr8.3:60319757-60319776	MS.gene37513:CDS	50.0%
	ATTGCCGCTATCGTCTGTAC+CGG	+	chr8.3:60319648-60319667	MS.gene37513:CDS	50.0%
!!	CAGCTGCAGATCGGTTTTTG+AGG	+	chr8.3:60319871-60319890	MS.gene37513:CDS	50.0%
	GGAGAGGAGGAAGAGAGGAA+GGG	-	chr8.3:60319590-60319609	None:intergenic	55.0%
	TATGATCAGTTCCGGCGCTG+GGG	-	chr8.3:60319734-60319753	None:intergenic	55.0%
	TCTCTCTGAGTGTCAGCGTC+GGG	+	chr8.3:60319758-60319777	MS.gene37513:CDS	55.0%
	TGGAGAGGAGGAAGAGAGGA+AGG	-	chr8.3:60319591-60319610	None:intergenic	55.0%
	TTGGTGGAGAGGAGGAAGAG+AGG	-	chr8.3:60319595-60319614	None:intergenic	55.0%
!	ACAGACGATAGCGGCAATGG+AGG	-	chr8.3:60319646-60319665	None:intergenic	55.0%
!	AGGAGGCATGGTGAGGATTG+AGG	-	chr8.3:60319626-60319645	None:intergenic	55.0%
!	GGAGGCATGGTGAGGATTGA+GGG	-	chr8.3:60319625-60319644	None:intergenic	55.0%
!	GGTACAGACGATAGCGGCAA+TGG	-	chr8.3:60319649-60319668	None:intergenic	55.0%
	CTCTCTGAGTGTCAGCGTCG+GGG	+	chr8.3:60319759-60319778	MS.gene37513:CDS	60.0%
	GCCTCCGGTACAGACGATAG+CGG	-	chr8.3:60319655-60319674	None:intergenic	60.0%
	TAGCGGCAATGGAGGAGGCA+TGG	-	chr8.3:60319638-60319657	None:intergenic	60.0%
!	ATGATCAGTTCCGGCGCTGG+GGG	-	chr8.3:60319733-60319752	None:intergenic	60.0%
!	GACGATAGCGGCAATGGAGG+AGG	-	chr8.3:60319643-60319662	None:intergenic	60.0%
!	GCAATGGAGGAGGCATGGTG+AGG	-	chr8.3:60319633-60319652	None:intergenic	60.0%
!	GTGTCAGCGTCGGGGAATTC+AGG	+	chr8.3:60319767-60319786	MS.gene37513:CDS	60.0%
!!	AGCGTTGACGGTGTAGCCTC+CGG	-	chr8.3:60319670-60319689	None:intergenic	60.0%
!!	CGTCGGGGAATTCAGGTTGC+CGG	+	chr8.3:60319774-60319793	MS.gene37513:CDS	60.0%
!!	GGAATTCAGGTTGCCGGAGC+TGG	+	chr8.3:60319780-60319799	MS.gene37513:CDS	60.0%
	GACGGAGGGCGGGAAGAAGT+TGG	-	chr8.3:60319701-60319720	None:intergenic	65.0%
	GCCGCTATCGTCTGTACCGG+AGG	+	chr8.3:60319651-60319670	MS.gene37513:CDS	65.0%
	GGTGCAGACAAGACGGAGGG+CGG	-	chr8.3:60319712-60319731	None:intergenic	65.0%
	GTGCAGACAAGACGGAGGGC+GGG	-	chr8.3:60319711-60319730	None:intergenic	65.0%
!	CGCTGGGGGTGCAGACAAGA+CGG	-	chr8.3:60319719-60319738	None:intergenic	65.0%
!	GGGGGTGCAGACAAGACGGA+GGG	-	chr8.3:60319715-60319734	None:intergenic	65.0%
!	TGGGGGTGCAGACAAGACGG+AGG	-	chr8.3:60319716-60319735	None:intergenic	65.0%
	CTTGTCTGCACCCCCAGCGC+CGG	+	chr8.3:60319720-60319739	MS.gene37513:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	60319456	60320017	60319456	ID=MS.gene37513
chr8.3	mRNA	60319456	60320017	60319456	ID=MS.gene37513.t1;Parent=MS.gene37513
chr8.3	exon	60319456	60319938	60319456	ID=MS.gene37513.t1.exon1;Parent=MS.gene37513.t1
chr8.3	CDS	60319456	60319938	60319456	ID=cds.MS.gene37513.t1;Parent=MS.gene37513.t1
chr8.3	exon	60319967	60320017	60319967	ID=MS.gene37513.t1.exon2;Parent=MS.gene37513.t1
chr8.3	CDS	60319967	60320017	60319967	ID=cds.MS.gene37513.t1;Parent=MS.gene37513.t1

Gene Sequence

>MS.gene37513

ATGGCAGATCTAAACAAAAGCATTGAAGAATCCCAAGAAAATAATAATCTCCCAAAAGTATTCCTTCATGGACCACCCGAATTCTCCTTCATCCTCCAACCAAATCCTTCTCACAATTTCCATATCTTAAACCCTTCCTCTCTTCCTCCTCTCCACCAATTCTTATCCCTCAATCCTCACCATGCCTCCTCCATTGCCGCTATCGTCTGTACCGGAGGCTACACCGTCAACGCTGATGTTCTCCAACTTCTTCCCGCCCTCCGTCTTGTCTGCACCCCCAGCGCCGGAACTGATCATATTGATCTCTCTGAGTGTCAGCGTCGGGGAATTCAGGTTGCCGGAGCTGGAAATCTTTTTTCTGAAGATGTTGCTGATATGGCTGTTGCTTTGTTGATTGATGTCAGCAGGAAAATTTCAGCTGCAGATCGGTTTTTGAGGAGACAAGTTCAGAATTCAACTTGGAAATTTCCACTTGGTTCTAAGGTATGCAATTATTTATTAATTTATGAAGAAAATATATTGCATAATATGTCTGTTTAATATGGTACAATATATTGCATAA

Protein sequence

>MS.gene37513.t1

MADLNKSIEESQENNNLPKVFLHGPPEFSFILQPNPSHNFHILNPSSLPPLHQFLSLNPHHASSIAAIVCTGGYTVNADVLQLLPALRLVCTPSAGTDHIDLSECQRRGIQVAGAGNLFSEDVADMAVALLIDVSRKISAADRFLRRQVQNSTWKFPLGSKKIYCIICLFNMVQYIA