Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41383.t1 | XP_003592328.1 | 99 | 104 | 1 | 0 | 1 | 104 | 1 | 104 | 3.90E-52 | 213.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41383.t1 | G7IC10 | 99.0 | 104 | 1 | 0 | 1 | 104 | 1 | 104 | 2.8e-52 | 213.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene41383 | 0.835984 | 1.22E-56 | -1.69E-46 |
| MS.gene050559 | MS.gene41383 | 0.832108 | 1.14E-55 | -1.69E-46 |
| MS.gene050561 | MS.gene41383 | 0.829932 | 3.90E-55 | -1.69E-46 |
| MS.gene050562 | MS.gene41383 | 0.828035 | 1.12E-54 | -1.69E-46 |
| MS.gene051247 | MS.gene41383 | 0.808635 | 2.81E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene41383 | 0.816779 | 4.64E-52 | -1.69E-46 |
| MS.gene051332 | MS.gene41383 | 0.828753 | 7.55E-55 | -1.69E-46 |
| MS.gene051490 | MS.gene41383 | 0.805335 | 1.40E-49 | -1.69E-46 |
| MS.gene051492 | MS.gene41383 | 0.830576 | 2.72E-55 | -1.69E-46 |
| MS.gene051744 | MS.gene41383 | 0.832937 | 7.11E-56 | -1.69E-46 |
| MS.gene052534 | MS.gene41383 | 0.82025 | 7.58E-53 | -1.69E-46 |
| MS.gene052582 | MS.gene41383 | 0.809783 | 1.60E-50 | -1.69E-46 |
| MS.gene052586 | MS.gene41383 | 0.82139 | 4.14E-53 | -1.69E-46 |
| MS.gene053006 | MS.gene41383 | 0.812646 | 3.82E-51 | -1.69E-46 |
| MS.gene053094 | MS.gene41383 | 0.872299 | 3.54E-67 | -1.69E-46 |
| MS.gene053166 | MS.gene41383 | 0.800059 | 1.72E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene41383 | 0.806841 | 6.76E-50 | -1.69E-46 |
| MS.gene053340 | MS.gene41383 | 0.811104 | 8.27E-51 | -1.69E-46 |
| MS.gene053963 | MS.gene41383 | 0.806566 | 7.73E-50 | -1.69E-46 |
| MS.gene05410 | MS.gene41383 | 0.816054 | 6.74E-52 | -1.69E-46 |
| MS.gene05414 | MS.gene41383 | 0.801081 | 1.07E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene41383 | 0.826383 | 2.80E-54 | -1.69E-46 |
| MS.gene05423 | MS.gene41383 | 0.801593 | 8.37E-49 | -1.69E-46 |
| MS.gene054576 | MS.gene41383 | 0.840578 | 7.99E-58 | -1.69E-46 |
| MS.gene054651 | MS.gene41383 | 0.817358 | 3.44E-52 | -1.69E-46 |
| MS.gene054892 | MS.gene41383 | 0.801213 | 1.00E-48 | -1.69E-46 |
| MS.gene054893 | MS.gene41383 | 0.801106 | 1.05E-48 | -1.69E-46 |
| MS.gene055586 | MS.gene41383 | 0.807971 | 3.90E-50 | -1.69E-46 |
| MS.gene055724 | MS.gene41383 | 0.817562 | 3.09E-52 | -1.69E-46 |
| MS.gene055841 | MS.gene41383 | 0.800455 | 1.43E-48 | -1.69E-46 |
| MS.gene056174 | MS.gene41383 | 0.806993 | 6.28E-50 | -1.69E-46 |
| MS.gene05686 | MS.gene41383 | 0.806104 | 9.67E-50 | -1.69E-46 |
| MS.gene057026 | MS.gene41383 | 0.81124 | 7.73E-51 | -1.69E-46 |
| MS.gene057071 | MS.gene41383 | 0.805426 | 1.34E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene41383 | 0.841866 | 3.66E-58 | -1.69E-46 |
| MS.gene058037 | MS.gene41383 | 0.807552 | 4.78E-50 | -1.69E-46 |
| MS.gene058294 | MS.gene41383 | 0.810829 | 9.49E-51 | -1.69E-46 |
| MS.gene058477 | MS.gene41383 | 0.809972 | 1.45E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene41383 | 0.804819 | 1.80E-49 | -1.69E-46 |
| MS.gene059272 | MS.gene41383 | 0.807907 | 4.02E-50 | -1.69E-46 |
| MS.gene059439 | MS.gene41383 | 0.803908 | 2.79E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene41383 | -0.804429 | 2.17E-49 | -1.69E-46 |
| MS.gene060310 | MS.gene41383 | 0.811341 | 7.35E-51 | -1.69E-46 |
| MS.gene060365 | MS.gene41383 | 0.811375 | 7.23E-51 | -1.69E-46 |
| MS.gene060373 | MS.gene41383 | 0.814715 | 1.34E-51 | -1.69E-46 |
| MS.gene060521 | MS.gene41383 | 0.80126 | 9.79E-49 | -1.69E-46 |
| MS.gene060716 | MS.gene41383 | 0.800543 | 1.37E-48 | -1.69E-46 |
| MS.gene060879 | MS.gene41383 | 0.811306 | 7.48E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41383.t1 | MTR_1g101680 | 99.038 | 104 | 1 | 0 | 1 | 104 | 1 | 104 | 6.79e-74 | 214 |
| MS.gene41383.t1 | MTR_1g101750 | 98.361 | 61 | 1 | 0 | 44 | 104 | 6 | 66 | 8.73e-41 | 129 |
| MS.gene41383.t1 | MTR_8g043660 | 61.458 | 96 | 31 | 2 | 6 | 96 | 18 | 112 | 1.39e-37 | 122 |
| MS.gene41383.t1 | MTR_8g032890 | 60.417 | 96 | 32 | 2 | 6 | 96 | 18 | 112 | 1.44e-35 | 117 |
| MS.gene41383.t1 | MTR_7g104250 | 63.333 | 90 | 31 | 1 | 10 | 97 | 6 | 95 | 4.10e-32 | 108 |
| MS.gene41383.t1 | MTR_1g060430 | 51.923 | 104 | 36 | 3 | 2 | 92 | 7 | 109 | 5.59e-31 | 106 |
| MS.gene41383.t1 | MTR_8g032940 | 63.043 | 92 | 29 | 1 | 6 | 92 | 18 | 109 | 1.02e-30 | 105 |
| MS.gene41383.t1 | MTR_5g038200 | 44.928 | 69 | 35 | 1 | 16 | 84 | 9 | 74 | 2.89e-15 | 65.1 |
| MS.gene41383.t1 | MTR_7g055590 | 74.286 | 35 | 9 | 0 | 59 | 93 | 461 | 495 | 1.31e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41383.t1 | AT4G25433 | 52.525 | 99 | 36 | 3 | 4 | 92 | 7 | 104 | 8.36e-30 | 103 |
| MS.gene41383.t1 | AT5G62150 | 58.947 | 95 | 37 | 2 | 7 | 101 | 10 | 102 | 9.28e-29 | 100 |
| MS.gene41383.t1 | AT3G52790 | 51.485 | 101 | 37 | 3 | 6 | 96 | 10 | 108 | 3.23e-28 | 99.0 |
Find 25 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTATTGGGCATGATATTAT+TGG | 0.221140 | 1.4:+78917608 | MS.gene41383:CDS |
| GTATTGTGCAATAGCGTTAT+TGG | 0.257022 | 1.4:+78917593 | MS.gene41383:CDS |
| CTTCAACAATTTCCTAGTAT+TGG | 0.339584 | 1.4:-78917847 | None:intergenic |
| TTATTGGGCATGATATTATT+GGG | 0.350044 | 1.4:+78917609 | MS.gene41383:CDS |
| GGATTCCTCTCTACAATAAA+CGG | 0.377759 | 1.4:-78917765 | None:intergenic |
| TATTGTGCAATAGCGTTATT+GGG | 0.380688 | 1.4:+78917594 | MS.gene41383:CDS |
| GTGGCTGAATTACAAGTTAG+AGG | 0.433397 | 1.4:+78917651 | MS.gene41383:CDS |
| AGACCAGGGAAAACATCATC+TGG | 0.456913 | 1.4:-78917801 | None:intergenic |
| TCATCACACCGACCAATACT+AGG | 0.469317 | 1.4:+78917835 | MS.gene41383:CDS |
| GGAAAACATCATCTGGATCA+TGG | 0.481353 | 1.4:-78917794 | None:intergenic |
| GATTCCTCTCTACAATAAAC+GGG | 0.500635 | 1.4:-78917764 | None:intergenic |
| CAAGTTAGAGGGAATAGTCT+TGG | 0.505805 | 1.4:+78917663 | MS.gene41383:CDS |
| AACAATTTCCTAGTATTGGT+CGG | 0.516529 | 1.4:-78917843 | None:intergenic |
| TCATCTGGATCATGGATATG+TGG | 0.554352 | 1.4:-78917786 | None:intergenic |
| CACAACATGGGTTCCTCTAG+AGG | 0.558317 | 1.4:+78917555 | None:intergenic |
| GAGATATATGTTGTGGGAGA+AGG | 0.558851 | 1.4:+78917705 | MS.gene41383:CDS |
| CTAGAGGCGATTCAGCATCA+TGG | 0.562112 | 1.4:+78917571 | MS.gene41383:CDS |
| ATGATTCTGATAACAAGACC+AGG | 0.590651 | 1.4:-78917816 | None:intergenic |
| TGTGATGAGATATATGTTGT+GGG | 0.600497 | 1.4:+78917699 | MS.gene41383:CDS |
| TGGCTGAATTACAAGTTAGA+GGG | 0.601217 | 1.4:+78917652 | MS.gene41383:CDS |
| TGATGCTGAATCGCCTCTAG+AGG | 0.610324 | 1.4:-78917568 | None:intergenic |
| GTGACCCGTTTATTGTAGAG+AGG | 0.616188 | 1.4:+78917760 | MS.gene41383:CDS |
| ATGTGATGAGATATATGTTG+TGG | 0.646471 | 1.4:+78917698 | MS.gene41383:CDS |
| ATCAATAAGAGAAAGTACAG+TGG | 0.690097 | 1.4:+78917632 | MS.gene41383:CDS |
| TGATTCTGATAACAAGACCA+GGG | 0.737080 | 1.4:-78917815 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTATTGGGCATGATATTATT+GGG | + | chr1.4:78917609-78917628 | MS.gene41383:CDS | 25.0% |
| AACACAATCAGTGAAAAATG+TGG | + | chr1.4:78917738-78917757 | MS.gene41383:CDS | 30.0% | |
| ATGTGATGAGATATATGTTG+TGG | + | chr1.4:78917698-78917717 | MS.gene41383:CDS | 30.0% | |
| CTTCAACAATTTCCTAGTAT+TGG | - | chr1.4:78917850-78917869 | None:intergenic | 30.0% | |
| GTTATTGGGCATGATATTAT+TGG | + | chr1.4:78917608-78917627 | MS.gene41383:CDS | 30.0% | |
| TATTGTGCAATAGCGTTATT+GGG | + | chr1.4:78917594-78917613 | MS.gene41383:CDS | 30.0% | |
| TGTGATGAGATATATGTTGT+GGG | + | chr1.4:78917699-78917718 | MS.gene41383:CDS | 30.0% | |
| ! | ATCAATAAGAGAAAGTACAG+TGG | + | chr1.4:78917632-78917651 | MS.gene41383:CDS | 30.0% |
| !! | AACAATTTCCTAGTATTGGT+CGG | - | chr1.4:78917846-78917865 | None:intergenic | 30.0% |
| ATGATTCTGATAACAAGACC+AGG | - | chr1.4:78917819-78917838 | None:intergenic | 35.0% | |
| GATTCCTCTCTACAATAAAC+GGG | - | chr1.4:78917767-78917786 | None:intergenic | 35.0% | |
| GGATTCCTCTCTACAATAAA+CGG | - | chr1.4:78917768-78917787 | None:intergenic | 35.0% | |
| GTATTGTGCAATAGCGTTAT+TGG | + | chr1.4:78917593-78917612 | MS.gene41383:CDS | 35.0% | |
| TGATTCTGATAACAAGACCA+GGG | - | chr1.4:78917818-78917837 | None:intergenic | 35.0% | |
| TGGCTGAATTACAAGTTAGA+GGG | + | chr1.4:78917652-78917671 | MS.gene41383:CDS | 35.0% | |
| CAAGTTAGAGGGAATAGTCT+TGG | + | chr1.4:78917663-78917682 | MS.gene41383:CDS | 40.0% | |
| GAGATATATGTTGTGGGAGA+AGG | + | chr1.4:78917705-78917724 | MS.gene41383:CDS | 40.0% | |
| GGAAAACATCATCTGGATCA+TGG | - | chr1.4:78917797-78917816 | None:intergenic | 40.0% | |
| GTGGCTGAATTACAAGTTAG+AGG | + | chr1.4:78917651-78917670 | MS.gene41383:CDS | 40.0% | |
| TCATCTGGATCATGGATATG+TGG | - | chr1.4:78917789-78917808 | None:intergenic | 40.0% | |
| AGACCAGGGAAAACATCATC+TGG | - | chr1.4:78917804-78917823 | None:intergenic | 45.0% | |
| GTGACCCGTTTATTGTAGAG+AGG | + | chr1.4:78917760-78917779 | MS.gene41383:CDS | 45.0% | |
| TCATCACACCGACCAATACT+AGG | + | chr1.4:78917835-78917854 | MS.gene41383:CDS | 45.0% | |
| ! | GATCCAGATGATGTTTTCCC+TGG | + | chr1.4:78917798-78917817 | MS.gene41383:CDS | 45.0% |
| CTAGAGGCGATTCAGCATCA+TGG | + | chr1.4:78917571-78917590 | MS.gene41383:CDS | 50.0% | |
| TGATGCTGAATCGCCTCTAG+AGG | - | chr1.4:78917571-78917590 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 78917561 | 78917875 | 78917561 | ID=MS.gene41383 |
| chr1.4 | mRNA | 78917561 | 78917875 | 78917561 | ID=MS.gene41383.t1;Parent=MS.gene41383 |
| chr1.4 | exon | 78917561 | 78917875 | 78917561 | ID=MS.gene41383.t1.exon1;Parent=MS.gene41383.t1 |
| chr1.4 | CDS | 78917561 | 78917875 | 78917561 | ID=cds.MS.gene41383.t1;Parent=MS.gene41383.t1 |
| Gene Sequence |
| Protein sequence |
