Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44502.t1 | XP_003608458.1 | 87.8 | 278 | 29 | 2 | 1 | 277 | 66 | 339 | 9.70E-34 | 154.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44502.t1 | G7JCT0 | 87.8 | 278 | 29 | 2 | 1 | 277 | 66 | 339 | 7.0e-34 | 154.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene44502 | 0.814412 | 1.56E-51 | -1.69E-46 |
| MS.gene049435 | MS.gene44502 | 0.817461 | 3.26E-52 | -1.69E-46 |
| MS.gene049501 | MS.gene44502 | 0.813806 | 2.12E-51 | -1.69E-46 |
| MS.gene049647 | MS.gene44502 | 0.801265 | 9.77E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene44502 | 0.834001 | 3.86E-56 | -1.69E-46 |
| MS.gene050807 | MS.gene44502 | 0.81022 | 1.28E-50 | -1.69E-46 |
| MS.gene050844 | MS.gene44502 | 0.810089 | 1.37E-50 | -1.69E-46 |
| MS.gene051247 | MS.gene44502 | 0.809867 | 1.53E-50 | -1.69E-46 |
| MS.gene052042 | MS.gene44502 | 0.808666 | 2.77E-50 | -1.69E-46 |
| MS.gene052323 | MS.gene44502 | 0.800407 | 1.46E-48 | -1.69E-46 |
| MS.gene052665 | MS.gene44502 | 0.810393 | 1.18E-50 | -1.69E-46 |
| MS.gene052773 | MS.gene44502 | 0.805169 | 1.52E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene44502 | 0.812711 | 3.70E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene44502 | 0.803846 | 2.87E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene44502 | 0.819162 | 1.34E-52 | -1.69E-46 |
| MS.gene053166 | MS.gene44502 | 0.800121 | 1.67E-48 | -1.69E-46 |
| MS.gene053221 | MS.gene44502 | 0.813028 | 3.15E-51 | -1.69E-46 |
| MS.gene054236 | MS.gene44502 | 0.809195 | 2.13E-50 | -1.69E-46 |
| MS.gene054291 | MS.gene44502 | -0.801984 | 6.96E-49 | -1.69E-46 |
| MS.gene054467 | MS.gene44502 | 0.850379 | 1.76E-60 | -1.69E-46 |
| MS.gene054468 | MS.gene44502 | 0.81284 | 3.46E-51 | -1.69E-46 |
| MS.gene054472 | MS.gene44502 | 0.808197 | 3.49E-50 | -1.69E-46 |
| MS.gene054559 | MS.gene44502 | 0.803664 | 3.13E-49 | -1.69E-46 |
| MS.gene054775 | MS.gene44502 | 0.817716 | 2.85E-52 | -1.69E-46 |
| MS.gene055356 | MS.gene44502 | 0.826516 | 2.60E-54 | -1.69E-46 |
| MS.gene055357 | MS.gene44502 | 0.841581 | 4.36E-58 | -1.69E-46 |
| MS.gene055358 | MS.gene44502 | 0.818328 | 2.08E-52 | -1.69E-46 |
| MS.gene055466 | MS.gene44502 | 0.818606 | 1.80E-52 | -1.69E-46 |
| MS.gene055818 | MS.gene44502 | 0.826671 | 2.39E-54 | -1.69E-46 |
| MS.gene055926 | MS.gene44502 | 0.809852 | 1.54E-50 | -1.69E-46 |
| MS.gene05604 | MS.gene44502 | 0.847619 | 1.03E-59 | -1.69E-46 |
| MS.gene056173 | MS.gene44502 | 0.829005 | 6.56E-55 | -1.69E-46 |
| MS.gene057693 | MS.gene44502 | 0.809699 | 1.66E-50 | -1.69E-46 |
| MS.gene057915 | MS.gene44502 | 0.802609 | 5.17E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene44502 | 0.801937 | 7.11E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene44502 | 0.800383 | 1.48E-48 | -1.69E-46 |
| MS.gene058636 | MS.gene44502 | 0.800992 | 1.11E-48 | -1.69E-46 |
| MS.gene059607 | MS.gene44502 | 0.812746 | 3.63E-51 | -1.69E-46 |
| MS.gene059725 | MS.gene44502 | 0.804179 | 2.45E-49 | -1.69E-46 |
| MS.gene059842 | MS.gene44502 | 0.810185 | 1.31E-50 | -1.69E-46 |
| MS.gene060137 | MS.gene44502 | -0.820721 | 5.90E-53 | -1.69E-46 |
| MS.gene060322 | MS.gene44502 | 0.814403 | 1.57E-51 | -1.69E-46 |
| MS.gene06052 | MS.gene44502 | 0.820399 | 7.00E-53 | -1.69E-46 |
| MS.gene060637 | MS.gene44502 | 0.801881 | 7.30E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene44502 | 0.808517 | 2.98E-50 | -1.69E-46 |
| MS.gene060685 | MS.gene44502 | 0.810527 | 1.10E-50 | -1.69E-46 |
| MS.gene060802 | MS.gene44502 | 0.81245 | 4.21E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene44502 | 0.839843 | 1.24E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44502.t1 | MTR_4g095320 | 96.763 | 278 | 4 | 2 | 1 | 277 | 66 | 339 | 0.0 | 535 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGTCTTGCAATCCTATTTC+TGG | 0.121525 | 8.1:-25925683 | None:intergenic |
| TTCTGTCTTGTATCCACTTT+TGG | 0.231382 | 8.1:-25925921 | None:intergenic |
| GACTTGGATGTGTGTGAAAT+TGG | 0.271057 | 8.1:-25925562 | None:intergenic |
| AAAATAGAAAATCATCAGTT+TGG | 0.305211 | 8.1:+25925630 | MS.gene44502:CDS |
| ATGAGTAAGAAGAACGAAAA+TGG | 0.355281 | 8.1:+25925800 | MS.gene44502:CDS |
| AAGGCAAAAGCAAGGGAAAA+AGG | 0.356195 | 8.1:+25925997 | MS.gene44502:CDS |
| AGGCACGTGATGGAAGAATT+TGG | 0.364176 | 8.1:-25925307 | None:intergenic |
| TACAACAACACCAATTGTTT+CGG | 0.381385 | 8.1:+25925517 | MS.gene44502:CDS |
| TCAACGAGTTTCGTAGCAAT+AGG | 0.398438 | 8.1:+25925453 | MS.gene44502:CDS |
| GTTGTCCCTACTCCAGAAAT+AGG | 0.400986 | 8.1:+25925671 | MS.gene44502:CDS |
| GTGAAATTGGTTTCTAACTT+TGG | 0.409356 | 8.1:-25925549 | None:intergenic |
| GCTGGTTTATTGACCACAAC+AGG | 0.425855 | 8.1:+25925344 | MS.gene44502:CDS |
| AAATAGAAAATCATCAGTTT+GGG | 0.443524 | 8.1:+25925631 | MS.gene44502:CDS |
| GTGATGGAAGAATTTGGCCT+TGG | 0.486671 | 8.1:-25925301 | None:intergenic |
| ATCAAAAGTGGTACCAAAAG+TGG | 0.490008 | 8.1:+25925908 | MS.gene44502:CDS |
| GCAATCCTATTTCTGGAGTA+GGG | 0.503974 | 8.1:-25925676 | None:intergenic |
| TGTGGTCAATAAACCAGCTT+GGG | 0.505911 | 8.1:-25925339 | None:intergenic |
| GAGTAGGGACAACACCTAAA+CGG | 0.508584 | 8.1:-25925661 | None:intergenic |
| TTGTGATTGTTCTATTGAAA+CGG | 0.520329 | 8.1:+25925883 | MS.gene44502:CDS |
| GCCTCATTCAGCTCCCAAGC+TGG | 0.528446 | 8.1:+25925326 | MS.gene44502:CDS |
| CACGTCGTAGAACATTTGAA+TGG | 0.535637 | 8.1:+25926026 | MS.gene44502:CDS |
| TGCAATCCTATTTCTGGAGT+AGG | 0.537145 | 8.1:-25925677 | None:intergenic |
| CAGAAGCAAAGGCAAAAGCA+AGG | 0.550325 | 8.1:+25925989 | MS.gene44502:CDS |
| ATACGAGGTGGTTTGTTGAG+TGG | 0.551188 | 8.1:+25925860 | MS.gene44502:CDS |
| ATTGAAACGGTAATCAAAAG+TGG | 0.552501 | 8.1:+25925896 | MS.gene44502:CDS |
| TGCAAGTCATGCTGATGAAG+AGG | 0.552902 | 8.1:+25926063 | MS.gene44502:CDS |
| ACCAGCTTGGGAGCTGAATG+AGG | 0.558380 | 8.1:-25925327 | None:intergenic |
| AATAGGATTGCAAGACCTCA+AGG | 0.561083 | 8.1:+25925688 | MS.gene44502:CDS |
| TTGTGGTCAATAAACCAGCT+TGG | 0.570440 | 8.1:-25925340 | None:intergenic |
| CGTGATGATGATCATGATCA+TGG | 0.580563 | 8.1:+25925380 | MS.gene44502:CDS |
| AGAAGCAAAGGCAAAAGCAA+GGG | 0.590737 | 8.1:+25925990 | MS.gene44502:CDS |
| TTGGAATTGTGATCCTGTTG+TGG | 0.598242 | 8.1:-25925357 | None:intergenic |
| CACTTAGTTGGTATACGAGG+TGG | 0.598581 | 8.1:+25925848 | MS.gene44502:CDS |
| AGAACTAGTGCTACTTGATG+TGG | 0.609982 | 8.1:-25925495 | None:intergenic |
| AGTGGAAGAAACAGAAGCAA+AGG | 0.629120 | 8.1:+25925978 | MS.gene44502:CDS |
| GAGCTGAATGAGGCACGTGA+TGG | 0.634782 | 8.1:-25925317 | None:intergenic |
| GATCATGATCATCATCACGT+TGG | 0.635353 | 8.1:-25925376 | None:intergenic |
| AAACACTTAGTTGGTATACG+AGG | 0.664458 | 8.1:+25925845 | MS.gene44502:CDS |
| TACTACTACTACGAACCTTG+AGG | 0.674315 | 8.1:-25925703 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAGAAAATCATCAGTT+TGG | + | chr8.1:25925630-25925649 | MS.gene44502:CDS | 20.0% |
| !! | AAATAGAAAATCATCAGTTT+GGG | + | chr8.1:25925631-25925650 | MS.gene44502:CDS | 20.0% |
| !!! | AGTACTTTTAATGATAAAAG+TGG | + | chr8.1:25925773-25925792 | MS.gene44502:CDS | 20.0% |
| !!! | TTTTGAGATATAGATTTTCT+TGG | - | chr8.1:25925614-25925633 | None:intergenic | 20.0% |
| ! | TTGTGATTGTTCTATTGAAA+CGG | + | chr8.1:25925883-25925902 | MS.gene44502:CDS | 25.0% |
| ATGAGTAAGAAGAACGAAAA+TGG | + | chr8.1:25925800-25925819 | MS.gene44502:CDS | 30.0% | |
| ATTGAAACGGTAATCAAAAG+TGG | + | chr8.1:25925896-25925915 | MS.gene44502:CDS | 30.0% | |
| TACAACAACACCAATTGTTT+CGG | + | chr8.1:25925517-25925536 | MS.gene44502:CDS | 30.0% | |
| ! | GTGAAATTGGTTTCTAACTT+TGG | - | chr8.1:25925552-25925571 | None:intergenic | 30.0% |
| !!! | CATGTTTTGAAACACTTAGT+TGG | + | chr8.1:25925836-25925855 | MS.gene44502:CDS | 30.0% |
| !!! | GATGATTTTAACTGAGGTTT+TGG | - | chr8.1:25925587-25925606 | None:intergenic | 30.0% |
| !!! | GATTTTGAAACCGAAACAAT+TGG | - | chr8.1:25925530-25925549 | None:intergenic | 30.0% |
| ATCAAAAGTGGTACCAAAAG+TGG | + | chr8.1:25925908-25925927 | MS.gene44502:CDS | 35.0% | |
| ! | AAACACTTAGTTGGTATACG+AGG | + | chr8.1:25925845-25925864 | MS.gene44502:CDS | 35.0% |
| ! | GTTTGGGAAATTTTCCGTTT+AGG | + | chr8.1:25925647-25925666 | MS.gene44502:CDS | 35.0% |
| ! | TTCTGTCTTGTATCCACTTT+TGG | - | chr8.1:25925924-25925943 | None:intergenic | 35.0% |
| !!! | GGACAAGATGATTTTAACTG+AGG | - | chr8.1:25925593-25925612 | None:intergenic | 35.0% |
| !!! | TTTAACTGAGGTTTTGGACT+TGG | - | chr8.1:25925581-25925600 | None:intergenic | 35.0% |
| AAGGCAAAAGCAAGGGAAAA+AGG | + | chr8.1:25925997-25926016 | MS.gene44502:CDS | 40.0% | |
| AATAGGATTGCAAGACCTCA+AGG | + | chr8.1:25925688-25925707 | MS.gene44502:CDS | 40.0% | |
| AGAAGCAAAGGCAAAAGCAA+GGG | + | chr8.1:25925990-25926009 | MS.gene44502:CDS | 40.0% | |
| AGGTCTTGCAATCCTATTTC+TGG | - | chr8.1:25925686-25925705 | None:intergenic | 40.0% | |
| AGTGGAAGAAACAGAAGCAA+AGG | + | chr8.1:25925978-25925997 | MS.gene44502:CDS | 40.0% | |
| CACGTCGTAGAACATTTGAA+TGG | + | chr8.1:25926026-25926045 | MS.gene44502:CDS | 40.0% | |
| CTGTGAAAGAAAAAGTGGAG+TGG | + | chr8.1:25925960-25925979 | MS.gene44502:CDS | 40.0% | |
| GACTTGGATGTGTGTGAAAT+TGG | - | chr8.1:25925565-25925584 | None:intergenic | 40.0% | |
| GATCATGATCATCATCACGT+TGG | - | chr8.1:25925379-25925398 | None:intergenic | 40.0% | |
| TACTACTACTACGAACCTTG+AGG | - | chr8.1:25925706-25925725 | None:intergenic | 40.0% | |
| TCAACGAGTTTCGTAGCAAT+AGG | + | chr8.1:25925453-25925472 | MS.gene44502:CDS | 40.0% | |
| TGTGGTCAATAAACCAGCTT+GGG | - | chr8.1:25925342-25925361 | None:intergenic | 40.0% | |
| TTGGAATTGTGATCCTGTTG+TGG | - | chr8.1:25925360-25925379 | None:intergenic | 40.0% | |
| TTGTGGTCAATAAACCAGCT+TGG | - | chr8.1:25925343-25925362 | None:intergenic | 40.0% | |
| ! | CGTGATGATGATCATGATCA+TGG | + | chr8.1:25925380-25925399 | MS.gene44502:CDS | 40.0% |
| ! | GCAGATGAAGTTTTCTTCCA+AGG | + | chr8.1:25925284-25925303 | MS.gene44502:CDS | 40.0% |
| !! | AGAACTAGTGCTACTTGATG+TGG | - | chr8.1:25925498-25925517 | None:intergenic | 40.0% |
| !! | GCAATCCTATTTCTGGAGTA+GGG | - | chr8.1:25925679-25925698 | None:intergenic | 40.0% |
| !! | TGCAATCCTATTTCTGGAGT+AGG | - | chr8.1:25925680-25925699 | None:intergenic | 40.0% |
| CAGAAGCAAAGGCAAAAGCA+AGG | + | chr8.1:25925989-25926008 | MS.gene44502:CDS | 45.0% | |
| GAGTAGGGACAACACCTAAA+CGG | - | chr8.1:25925664-25925683 | None:intergenic | 45.0% | |
| GCACGCTGTGAAAGAAAAAG+TGG | + | chr8.1:25925955-25925974 | MS.gene44502:CDS | 45.0% | |
| GTTGTCCCTACTCCAGAAAT+AGG | + | chr8.1:25925671-25925690 | MS.gene44502:CDS | 45.0% | |
| TGCAAGTCATGCTGATGAAG+AGG | + | chr8.1:25926063-25926082 | MS.gene44502:CDS | 45.0% | |
| ! | AGGCACGTGATGGAAGAATT+TGG | - | chr8.1:25925310-25925329 | None:intergenic | 45.0% |
| ! | CACTTAGTTGGTATACGAGG+TGG | + | chr8.1:25925848-25925867 | MS.gene44502:CDS | 45.0% |
| ! | GCTGGTTTATTGACCACAAC+AGG | + | chr8.1:25925344-25925363 | MS.gene44502:CDS | 45.0% |
| ! | GTGATGGAAGAATTTGGCCT+TGG | - | chr8.1:25925304-25925323 | None:intergenic | 45.0% |
| !! | ATACGAGGTGGTTTGTTGAG+TGG | + | chr8.1:25925860-25925879 | MS.gene44502:CDS | 45.0% |
| ACCAGCTTGGGAGCTGAATG+AGG | - | chr8.1:25925330-25925349 | None:intergenic | 55.0% | |
| GAGCTGAATGAGGCACGTGA+TGG | - | chr8.1:25925320-25925339 | None:intergenic | 55.0% | |
| GCCTCATTCAGCTCCCAAGC+TGG | + | chr8.1:25925326-25925345 | MS.gene44502:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 25925275 | 25926108 | 25925275 | ID=MS.gene44502 |
| chr8.1 | mRNA | 25925275 | 25926108 | 25925275 | ID=MS.gene44502.t1;Parent=MS.gene44502 |
| chr8.1 | exon | 25925275 | 25926108 | 25925275 | ID=MS.gene44502.t1.exon1;Parent=MS.gene44502.t1 |
| chr8.1 | CDS | 25925275 | 25926108 | 25925275 | ID=cds.MS.gene44502.t1;Parent=MS.gene44502.t1 |
| Gene Sequence |
| Protein sequence |
