Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44844.t1 | XP_024639822.1 | 99.3 | 566 | 4 | 0 | 1 | 566 | 1 | 566 | 0.00E+00 | 1163.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44844.t1 | G7KE15 | 99.3 | 566 | 4 | 0 | 1 | 566 | 1 | 566 | 0.0e+00 | 1163.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049190 | MS.gene44844 | 0.805676 | 1.19E-49 | -1.69E-46 |
| MS.gene049418 | MS.gene44844 | 0.853587 | 2.17E-61 | -1.69E-46 |
| MS.gene049807 | MS.gene44844 | 0.828556 | 8.42E-55 | -1.69E-46 |
| MS.gene049887 | MS.gene44844 | 0.858684 | 6.96E-63 | -1.69E-46 |
| MS.gene050194 | MS.gene44844 | 0.818545 | 1.85E-52 | -1.69E-46 |
| MS.gene050630 | MS.gene44844 | 0.813149 | 2.96E-51 | -1.69E-46 |
| MS.gene050689 | MS.gene44844 | 0.815394 | 9.46E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene44844 | 0.812117 | 4.98E-51 | -1.69E-46 |
| MS.gene051042 | MS.gene44844 | 0.816355 | 5.77E-52 | -1.69E-46 |
| MS.gene051067 | MS.gene44844 | 0.82044 | 6.85E-53 | -1.69E-46 |
| MS.gene051234 | MS.gene44844 | 0.805603 | 1.23E-49 | -1.69E-46 |
| MS.gene051478 | MS.gene44844 | 0.800762 | 1.24E-48 | -1.69E-46 |
| MS.gene051719 | MS.gene44844 | 0.80795 | 3.93E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene44844 | 0.803994 | 2.67E-49 | -1.69E-46 |
| MS.gene051781 | MS.gene44844 | 0.834598 | 2.73E-56 | -1.69E-46 |
| MS.gene051784 | MS.gene44844 | 0.825145 | 5.50E-54 | -1.69E-46 |
| MS.gene051787 | MS.gene44844 | 0.820435 | 6.87E-53 | -1.69E-46 |
| MS.gene051885 | MS.gene44844 | 0.850188 | 2.00E-60 | -1.69E-46 |
| MS.gene052133 | MS.gene44844 | 0.80004 | 1.74E-48 | -1.69E-46 |
| MS.gene052155 | MS.gene44844 | -0.820536 | 6.51E-53 | -1.69E-46 |
| MS.gene052516 | MS.gene44844 | 0.803631 | 3.18E-49 | -1.69E-46 |
| MS.gene052731 | MS.gene44844 | 0.812716 | 3.69E-51 | -1.69E-46 |
| MS.gene052732 | MS.gene44844 | 0.856891 | 2.37E-62 | -1.69E-46 |
| MS.gene053144 | MS.gene44844 | 0.917057 | 8.43E-86 | -1.69E-46 |
| MS.gene053174 | MS.gene44844 | 0.819559 | 1.09E-52 | -1.69E-46 |
| MS.gene053536 | MS.gene44844 | 0.848592 | 5.56E-60 | -1.69E-46 |
| MS.gene053538 | MS.gene44844 | 0.854414 | 1.25E-61 | -1.69E-46 |
| MS.gene053581 | MS.gene44844 | 0.856248 | 3.66E-62 | -1.69E-46 |
| MS.gene053918 | MS.gene44844 | 0.806452 | 8.17E-50 | -1.69E-46 |
| MS.gene054550 | MS.gene44844 | 0.810993 | 8.74E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene44844 | 0.899775 | 1.42E-77 | -1.69E-46 |
| MS.gene055465 | MS.gene44844 | 0.820911 | 5.34E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene44844 | 0.818189 | 2.23E-52 | -1.69E-46 |
| MS.gene055468 | MS.gene44844 | 0.8341 | 3.64E-56 | -1.69E-46 |
| MS.gene055537 | MS.gene44844 | 0.839976 | 1.15E-57 | -1.69E-46 |
| MS.gene055580 | MS.gene44844 | -0.882985 | 6.58E-71 | -1.69E-46 |
| MS.gene055764 | MS.gene44844 | 0.81016 | 1.32E-50 | -1.69E-46 |
| MS.gene055890 | MS.gene44844 | 0.809304 | 2.02E-50 | -1.69E-46 |
| MS.gene055891 | MS.gene44844 | 0.829993 | 3.77E-55 | -1.69E-46 |
| MS.gene055979 | MS.gene44844 | 0.801225 | 9.96E-49 | -1.69E-46 |
| MS.gene056043 | MS.gene44844 | -0.879523 | 1.16E-69 | -1.69E-46 |
| MS.gene056075 | MS.gene44844 | 0.829469 | 5.06E-55 | -1.69E-46 |
| MS.gene056368 | MS.gene44844 | 0.817508 | 3.18E-52 | -1.69E-46 |
| MS.gene056396 | MS.gene44844 | 0.857495 | 1.57E-62 | -1.69E-46 |
| MS.gene058945 | MS.gene44844 | 0.805589 | 1.24E-49 | -1.69E-46 |
| MS.gene05894 | MS.gene44844 | 0.822616 | 2.15E-53 | -1.69E-46 |
| MS.gene059424 | MS.gene44844 | 0.837511 | 4.98E-57 | -1.69E-46 |
| MS.gene060194 | MS.gene44844 | 0.832976 | 6.95E-56 | -1.69E-46 |
| MS.gene060587 | MS.gene44844 | 0.818098 | 2.34E-52 | -1.69E-46 |
| MS.gene060603 | MS.gene44844 | 0.800142 | 1.66E-48 | -1.69E-46 |
| MS.gene060884 | MS.gene44844 | 0.83633 | 9.97E-57 | -1.69E-46 |
| MS.gene060886 | MS.gene44844 | 0.841029 | 6.09E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene44844 | MS.gene023532 | PPI |
| MS.gene44844 | MS.gene08769 | PPI |
| MS.gene09087 | MS.gene44844 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44844.t1 | MTR_5g069410 | 99.293 | 566 | 4 | 0 | 1 | 566 | 1 | 566 | 0.0 | 1179 |
| MS.gene44844.t1 | MTR_8g085780 | 48.325 | 567 | 265 | 6 | 14 | 561 | 16 | 573 | 0.0 | 565 |
| MS.gene44844.t1 | MTR_8g106395 | 23.948 | 309 | 190 | 9 | 271 | 553 | 75 | 364 | 6.52e-16 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44844.t1 | AT4G12700 | 74.427 | 567 | 134 | 5 | 1 | 564 | 1 | 559 | 0.0 | 895 |
| MS.gene44844.t1 | AT2G04280 | 74.868 | 569 | 135 | 6 | 1 | 564 | 1 | 566 | 0.0 | 895 |
| MS.gene44844.t1 | AT4G08810 | 47.965 | 565 | 254 | 5 | 16 | 561 | 9 | 552 | 0.0 | 521 |
| MS.gene44844.t1 | AT3G56750 | 23.718 | 312 | 186 | 11 | 271 | 553 | 87 | 375 | 3.66e-16 | 81.3 |
| MS.gene44844.t1 | AT2G41150 | 22.581 | 310 | 193 | 10 | 271 | 553 | 87 | 376 | 3.96e-16 | 81.3 |
Find 126 sgRNAs with CRISPR-Local
Find 152 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACGTTTAGTGTTGTTAGTA+TGG | 0.260774 | 5.2:+58656675 | MS.gene44844:CDS |
| CAATAGCTTCAAGGTTAAAC+TGG | 0.270199 | 5.2:+58657346 | MS.gene44844:CDS |
| GGTTTCAGACCAGAGAATTT+AGG | 0.279478 | 5.2:+58656121 | MS.gene44844:CDS |
| TTCATCTGGTCGAGGATTAC+AGG | 0.297935 | 5.2:+58657151 | MS.gene44844:CDS |
| GCTTTAATCATGAGAAAGTT+TGG | 0.298138 | 5.2:+58657207 | MS.gene44844:CDS |
| TTTATGTGGGTGGTGGAGAT+AGG | 0.323850 | 5.2:+58656875 | MS.gene44844:CDS |
| AAAGGCCATGGCATTTGATA+TGG | 0.327645 | 5.2:+58657292 | MS.gene44844:CDS |
| TAAATTTGGGAAACCTGTTC+CGG | 0.333058 | 5.2:+58656540 | MS.gene44844:CDS |
| ACTCAGTGTGAGGGGAATTC+AGG | 0.333650 | 5.2:+58656436 | MS.gene44844:CDS |
| CCATAACCAATGTTCGGTTT+AGG | 0.341807 | 5.2:-58656957 | None:intergenic |
| AGTGTTTGTTATTGGTGTTT+TGG | 0.342766 | 5.2:+58656183 | MS.gene44844:CDS |
| ACTCTATACCAATAGTTATC+AGG | 0.346554 | 5.2:-58657240 | None:intergenic |
| GGATGAAACAAAGGATCTTC+AGG | 0.354420 | 5.2:-58656235 | None:intergenic |
| TTCACATCCATAAGTTTCAT+TGG | 0.367179 | 5.2:-58657180 | None:intergenic |
| AGAGAAGACATCGTCGAATC+TGG | 0.368044 | 5.2:+58656756 | MS.gene44844:CDS |
| GGTCACACCAATGAAACTTA+TGG | 0.375350 | 5.2:+58657173 | MS.gene44844:CDS |
| TGTTGGACAAGGATCAGTTT+TGG | 0.376150 | 5.2:+58657088 | MS.gene44844:CDS |
| TCAACGAGTATAAGGAACTT+TGG | 0.387999 | 5.2:+58657574 | MS.gene44844:CDS |
| CCTAAACCGAACATTGGTTA+TGG | 0.388632 | 5.2:+58656957 | MS.gene44844:CDS |
| CAGAATACTATGGCCATGAT+TGG | 0.392276 | 5.2:+58656145 | MS.gene44844:CDS |
| GTCAATGCGTTGCCGGTTGT+TGG | 0.394102 | 5.2:+58656784 | MS.gene44844:CDS |
| GAGGTATGTGCATCAAGATT+TGG | 0.397669 | 5.2:-58657426 | None:intergenic |
| CAACGAGTATAAGGAACTTT+GGG | 0.400281 | 5.2:+58657575 | MS.gene44844:CDS |
| GTGATATGGCGTGGAGGTAT+AGG | 0.404031 | 5.2:+58656581 | MS.gene44844:CDS |
| TGCACTTCTCTCAACCTTAC+GGG | 0.405105 | 5.2:+58657455 | MS.gene44844:CDS |
| AGGTAAATACTTGGTTTATG+TGG | 0.409434 | 5.2:+58656861 | MS.gene44844:CDS |
| GGATCAGGTCAATGCGTTGC+CGG | 0.418320 | 5.2:+58656777 | MS.gene44844:CDS |
| TAACAGTGATTAAGAATGTT+GGG | 0.418456 | 5.2:+58656073 | None:intergenic |
| GAATGATAGTACCTGTGATA+TGG | 0.418806 | 5.2:+58656567 | MS.gene44844:CDS |
| GCTACTAATGAGTCTGTGTT+TGG | 0.419365 | 5.2:+58656349 | MS.gene44844:CDS |
| TCAGGGTTCTTACAATCAAT+AGG | 0.421487 | 5.2:-58656460 | None:intergenic |
| ATGGAAATCGAGGCGATTGA+TGG | 0.422328 | 5.2:+58657311 | MS.gene44844:CDS |
| TGGAGTTTCTTGTGTGCTCT+AGG | 0.424785 | 5.2:+58656922 | MS.gene44844:CDS |
| GGCGTTCCAGTAGAATTTGA+TGG | 0.427805 | 5.2:+58657642 | MS.gene44844:CDS |
| AAATTTGGGAAACCTGTTCC+GGG | 0.431548 | 5.2:+58656541 | MS.gene44844:CDS |
| GATAACAGTGTTGTGAAAAC+TGG | 0.435692 | 5.2:+58656313 | MS.gene44844:CDS |
| ACGTTTAGTGTTGTTAGTAT+GGG | 0.436986 | 5.2:+58656676 | MS.gene44844:CDS |
| TAGTGTCTGCAATAGCTTCA+AGG | 0.440816 | 5.2:+58657337 | MS.gene44844:CDS |
| CGTTTAGTGTTGTTAGTATG+GGG | 0.454205 | 5.2:+58656677 | MS.gene44844:CDS |
| AATGTTGTAATCTTAGTTGA+AGG | 0.455380 | 5.2:-58656256 | None:intergenic |
| TTCACAAATTCTCCAACAAC+CGG | 0.456505 | 5.2:-58656796 | None:intergenic |
| GTATTCTGACCTAAATTCTC+TGG | 0.456518 | 5.2:-58656130 | None:intergenic |
| ATGTAACCATCAAATTCTAC+TGG | 0.457405 | 5.2:-58657648 | None:intergenic |
| AATTTACACTTCATCGAAGC+AGG | 0.462106 | 5.2:+58657002 | MS.gene44844:CDS |
| AGATGGGATGAATCTTCATC+TGG | 0.466104 | 5.2:+58657137 | MS.gene44844:CDS |
| GAATTTAGGTCAGAATACTA+TGG | 0.467250 | 5.2:+58656135 | MS.gene44844:CDS |
| GAACTTTCCGACTCAACTAC+AGG | 0.470123 | 5.2:-58656829 | None:intergenic |
| AGGCGAGGAACAAAGAGCTT+TGG | 0.473800 | 5.2:+58657403 | MS.gene44844:CDS |
| TCATGTTGTGAGAGGGGAAA+AGG | 0.479362 | 5.2:+58657383 | MS.gene44844:CDS |
| CTATCATTCGAACCCGGAAC+AGG | 0.480882 | 5.2:-58656553 | None:intergenic |
| TTCGTCAACCTTGTCCCGTA+AGG | 0.484068 | 5.2:-58657469 | None:intergenic |
| ATCAGACTTAAAAGTAGCAT+TGG | 0.486545 | 5.2:-58656293 | None:intergenic |
| AAGGTTGAGAGAAGTGCATC+GGG | 0.490128 | 5.2:-58657450 | None:intergenic |
| TTCTCTGGTCTGAAACCTCC+AGG | 0.490679 | 5.2:-58656115 | None:intergenic |
| ATGCACTTCTCTCAACCTTA+CGG | 0.491937 | 5.2:+58657454 | MS.gene44844:CDS |
| GCCGCAGCCTTCCCATCCTT+CGG | 0.493260 | 5.2:-58656604 | None:intergenic |
| ATTTAGTCAAGGTAAATACT+TGG | 0.498121 | 5.2:+58656852 | MS.gene44844:CDS |
| TTTGGGCAGATTGGAGTAAG+TGG | 0.499384 | 5.2:+58657106 | MS.gene44844:CDS |
| TCAAATCCATAACCAATGTT+CGG | 0.500832 | 5.2:-58656963 | None:intergenic |
| GACATCGTCGAATCTGGATC+AGG | 0.504087 | 5.2:+58656762 | MS.gene44844:CDS |
| ATGTCGAGCCTGATAACTAT+TGG | 0.506139 | 5.2:+58657232 | MS.gene44844:CDS |
| AAAACAAACATTCCCAATCA+TGG | 0.507885 | 5.2:-58656158 | None:intergenic |
| CACTTCATCGAAGCAGGATG+AGG | 0.511044 | 5.2:+58657008 | MS.gene44844:CDS |
| GGTAAATACTTGGTTTATGT+GGG | 0.513383 | 5.2:+58656862 | MS.gene44844:CDS |
| GGATCAAAGAAAGATGCATC+AGG | 0.513790 | 5.2:-58657522 | None:intergenic |
| AGAATACTATGGCCATGATT+GGG | 0.518270 | 5.2:+58656146 | MS.gene44844:CDS |
| CTGGTATGAATGCGAGGAAG+AGG | 0.518699 | 5.2:+58656710 | MS.gene44844:CDS |
| ATCTTTCTTTGATCCTCTGA+AGG | 0.524319 | 5.2:+58657530 | MS.gene44844:CDS |
| AGGAAGAAGAATCAGAAGGC+TGG | 0.529198 | 5.2:+58656730 | MS.gene44844:CDS |
| GAAGGAGGCGGCGTCTGTGT+TGG | 0.531021 | 5.2:+58657071 | MS.gene44844:CDS |
| TGGAGGTATAGGCCGAAGGA+TGG | 0.536112 | 5.2:+58656592 | MS.gene44844:CDS |
| TACTTGGTTTATGTGGGTGG+TGG | 0.539901 | 5.2:+58656868 | MS.gene44844:CDS |
| TCTCTCAACCTTACGGGACA+AGG | 0.545064 | 5.2:+58657461 | MS.gene44844:CDS |
| GATACCATCCTTGCAATCAC+TGG | 0.552369 | 5.2:-58657739 | None:intergenic |
| TTGACCAGTGATTGCAAGGA+TGG | 0.552652 | 5.2:+58657735 | MS.gene44844:CDS |
| AAAGCATGAATCACTTCTTG+TGG | 0.553481 | 5.2:+58656902 | MS.gene44844:CDS |
| GTTTAGTGTTGTTAGTATGG+GGG | 0.554390 | 5.2:+58656678 | MS.gene44844:CDS |
| CGGTGAGACTAATGAGGCTG+AGG | 0.557626 | 5.2:+58656411 | MS.gene44844:CDS |
| GGCATTTGATATGGAAATCG+AGG | 0.561183 | 5.2:+58657301 | MS.gene44844:CDS |
| GTTCATTTGTAGCAATGTAG+AGG | 0.561206 | 5.2:-58657500 | None:intergenic |
| AAATACTTGGTTTATGTGGG+TGG | 0.565916 | 5.2:+58656865 | MS.gene44844:CDS |
| GATGTTGATAACTCGGTTAG+CGG | 0.568706 | 5.2:+58656391 | MS.gene44844:CDS |
| TATGACTGATGTTGATAACT+CGG | 0.569096 | 5.2:+58656384 | MS.gene44844:CDS |
| GTATAGGCCGAAGGATGGGA+AGG | 0.570064 | 5.2:+58656597 | MS.gene44844:CDS |
| ACTCAGTTCATGTTGTGAGA+GGG | 0.571566 | 5.2:+58657376 | MS.gene44844:CDS |
| GACTCAGTTCATGTTGTGAG+AGG | 0.575510 | 5.2:+58657375 | MS.gene44844:CDS |
| AATCTGTTGTTCAAAGGCCA+TGG | 0.581613 | 5.2:+58657280 | MS.gene44844:CDS |
| GTTGAATACTTATCCTTCAG+AGG | 0.583499 | 5.2:-58657543 | None:intergenic |
| CAGCCTCCCTGTAGTTGAGT+CGG | 0.583590 | 5.2:+58656822 | MS.gene44844:CDS |
| CTCAGTTCATGTTGTGAGAG+GGG | 0.585625 | 5.2:+58657377 | MS.gene44844:CDS |
| TAAGGTTGAGAGAAGTGCAT+CGG | 0.587272 | 5.2:-58657451 | None:intergenic |
| ATACCTCCACGCCATATCAC+AGG | 0.588167 | 5.2:-58656578 | None:intergenic |
| AATAGCTTCAAGGTTAAACT+GGG | 0.590944 | 5.2:+58657347 | MS.gene44844:CDS |
| GAGACAACAAAACTCAATAA+CGG | 0.592530 | 5.2:+58657621 | MS.gene44844:CDS |
| AGACAGAATCTGTTGTTCAA+AGG | 0.593771 | 5.2:+58657274 | MS.gene44844:CDS |
| GGAGGTATAGGCCGAAGGAT+GGG | 0.595782 | 5.2:+58656593 | MS.gene44844:CDS |
| TCGGAAAGTTCATTTAGTCA+AGG | 0.597241 | 5.2:+58656841 | MS.gene44844:CDS |
| TCATCTGGTCGAGGATTACA+GGG | 0.598635 | 5.2:+58657152 | MS.gene44844:CDS |
| GCCGAAGGATGGGAAGGCTG+CGG | 0.599090 | 5.2:+58656603 | MS.gene44844:CDS |
| TGATTTGACCAGTGATTGCA+AGG | 0.600244 | 5.2:+58657731 | MS.gene44844:CDS |
| ATCATACTGGTATGAATGCG+AGG | 0.603806 | 5.2:+58656704 | MS.gene44844:CDS |
| TCATCGAAGCAGGATGAGGA+AGG | 0.609494 | 5.2:+58657012 | MS.gene44844:CDS |
| GGCGGCGTCTGTGTTGGACA+AGG | 0.610283 | 5.2:+58657077 | MS.gene44844:CDS |
| TTAGTTGAAGGATGAAACAA+AGG | 0.610506 | 5.2:-58656244 | None:intergenic |
| GATGAATCTTCATCTGGTCG+AGG | 0.613587 | 5.2:+58657143 | MS.gene44844:CDS |
| CTGAGGTGACTCAGTGTGAG+GGG | 0.614034 | 5.2:+58656428 | MS.gene44844:CDS |
| GGCGTGGAGGTATAGGCCGA+AGG | 0.618753 | 5.2:+58656588 | MS.gene44844:CDS |
| GATTTCCATATCAAATGCCA+TGG | 0.621642 | 5.2:-58657297 | None:intergenic |
| AACTTTCCGACTCAACTACA+GGG | 0.628434 | 5.2:-58656828 | None:intergenic |
| GGTTAGCGGTGAGACTAATG+AGG | 0.629932 | 5.2:+58656405 | MS.gene44844:CDS |
| AGTGATTAAGAATGTTGGGT+CGG | 0.631650 | 5.2:+58656077 | None:intergenic |
| TCAGTACCTAAACCGAACAT+TGG | 0.632278 | 5.2:+58656951 | MS.gene44844:CDS |
| TTTGGGATGAGACTAGTGAA+TGG | 0.636894 | 5.2:+58657592 | MS.gene44844:CDS |
| GCTGAGGTGACTCAGTGTGA+GGG | 0.644517 | 5.2:+58656427 | MS.gene44844:CDS |
| TTGTGAGAGGGGAAAAGGCG+AGG | 0.644621 | 5.2:+58657388 | MS.gene44844:CDS |
| GGTTGAGAGAAGTGCATCGG+GGG | 0.648446 | 5.2:-58657448 | None:intergenic |
| CAGGTACTATCATTCGAACC+CGG | 0.649356 | 5.2:-58656559 | None:intergenic |
| TATTGGTATAGAGTCTGCGA+AGG | 0.653670 | 5.2:+58657249 | MS.gene44844:CDS |
| GAAGAGGAAGAAGAATCAGA+AGG | 0.658325 | 5.2:+58656726 | MS.gene44844:CDS |
| GGCTGAGGTGACTCAGTGTG+AGG | 0.673349 | 5.2:+58656426 | MS.gene44844:CDS |
| ATAGTACCTGTGATATGGCG+TGG | 0.678671 | 5.2:+58656572 | MS.gene44844:CDS |
| CAGTGTTGTGAAAACTGGTG+AGG | 0.689827 | 5.2:+58656318 | MS.gene44844:CDS |
| TTACGGGACAAGGTTGACGA+AGG | 0.696825 | 5.2:+58657471 | MS.gene44844:CDS |
| GTACCTGTGATATGGCGTGG+AGG | 0.742102 | 5.2:+58656575 | MS.gene44844:CDS |
| AGGTTGAGAGAAGTGCATCG+GGG | 0.747201 | 5.2:-58657449 | None:intergenic |
| TTTCCGACTCAACTACAGGG+AGG | 0.771122 | 5.2:-58656825 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGTTCATTTTTATAAATTT+GGG | + | chr5.2:58656527-58656546 | MS.gene44844:CDS | 10.0% |
| !!! | GATGTTCATTTTTATAAATT+TGG | + | chr5.2:58656526-58656545 | MS.gene44844:CDS | 15.0% |
| ! | AATGTTGTAATCTTAGTTGA+AGG | - | chr5.2:58656259-58656278 | None:intergenic | 25.0% |
| ! | ATCATCAAATGAAAAACTTC+AGG | - | chr5.2:58656481-58656500 | None:intergenic | 25.0% |
| ! | ATTTAGTCAAGGTAAATACT+TGG | + | chr5.2:58656852-58656871 | MS.gene44844:CDS | 25.0% |
| ! | TCATCAAATGAAAAACTTCA+GGG | - | chr5.2:58656480-58656499 | None:intergenic | 25.0% |
| !!! | AAGTTTTTCATTTGATGATG+AGG | + | chr5.2:58656482-58656501 | MS.gene44844:CDS | 25.0% |
| !!! | AGTTTTTCATTTGATGATGA+GGG | + | chr5.2:58656483-58656502 | MS.gene44844:CDS | 25.0% |
| !!! | TGTTTTTCAGTGTTTGTTAT+TGG | + | chr5.2:58656175-58656194 | MS.gene44844:CDS | 25.0% |
| AAAACAAACATTCCCAATCA+TGG | - | chr5.2:58656161-58656180 | None:intergenic | 30.0% | |
| AATAGCTTCAAGGTTAAACT+GGG | + | chr5.2:58657347-58657366 | MS.gene44844:CDS | 30.0% | |
| ACTCTATACCAATAGTTATC+AGG | - | chr5.2:58657243-58657262 | None:intergenic | 30.0% | |
| AGGTAAATACTTGGTTTATG+TGG | + | chr5.2:58656861-58656880 | MS.gene44844:CDS | 30.0% | |
| ATCAGACTTAAAAGTAGCAT+TGG | - | chr5.2:58656296-58656315 | None:intergenic | 30.0% | |
| ATGTAACCATCAAATTCTAC+TGG | - | chr5.2:58657651-58657670 | None:intergenic | 30.0% | |
| CAAAAACACTTCTGTATCAA+TGG | - | chr5.2:58657682-58657701 | None:intergenic | 30.0% | |
| GAATTTAGGTCAGAATACTA+TGG | + | chr5.2:58656135-58656154 | MS.gene44844:CDS | 30.0% | |
| GAGACAACAAAACTCAATAA+CGG | + | chr5.2:58657621-58657640 | MS.gene44844:CDS | 30.0% | |
| GCTTTAATCATGAGAAAGTT+TGG | + | chr5.2:58657207-58657226 | MS.gene44844:CDS | 30.0% | |
| GGTAAATACTTGGTTTATGT+GGG | + | chr5.2:58656862-58656881 | MS.gene44844:CDS | 30.0% | |
| TCAAATCCATAACCAATGTT+CGG | - | chr5.2:58656966-58656985 | None:intergenic | 30.0% | |
| TTAGTTGAAGGATGAAACAA+AGG | - | chr5.2:58656247-58656266 | None:intergenic | 30.0% | |
| TTCACATCCATAAGTTTCAT+TGG | - | chr5.2:58657183-58657202 | None:intergenic | 30.0% | |
| ! | ACGTTTAGTGTTGTTAGTAT+GGG | + | chr5.2:58656676-58656695 | MS.gene44844:CDS | 30.0% |
| ! | CGTTTTATAAGGATTATAGG+AGG | + | chr5.2:58656626-58656645 | MS.gene44844:CDS | 30.0% |
| ! | TACGTTTAGTGTTGTTAGTA+TGG | + | chr5.2:58656675-58656694 | MS.gene44844:CDS | 30.0% |
| ! | TCATTTTCTCAACGAGTATA+AGG | + | chr5.2:58657566-58657585 | MS.gene44844:CDS | 30.0% |
| !! | CTTTGATTTTGAGCATTTGA+AGG | + | chr5.2:58657053-58657072 | MS.gene44844:CDS | 30.0% |
| !! | TATGACTGATGTTGATAACT+CGG | + | chr5.2:58656384-58656403 | MS.gene44844:CDS | 30.0% |
| !!! | AGTGTTTGTTATTGGTGTTT+TGG | + | chr5.2:58656183-58656202 | MS.gene44844:CDS | 30.0% |
| !!! | GTTATTGAGTTTTGTTGTCT+CGG | - | chr5.2:58657622-58657641 | None:intergenic | 30.0% |
| !!! | TGTTTTGGTTTTCACTATCA+TGG | + | chr5.2:58656198-58656217 | MS.gene44844:CDS | 30.0% |
| AAAGCATGAATCACTTCTTG+TGG | + | chr5.2:58656902-58656921 | MS.gene44844:CDS | 35.0% | |
| AAATACTTGGTTTATGTGGG+TGG | + | chr5.2:58656865-58656884 | MS.gene44844:CDS | 35.0% | |
| AATTTACACTTCATCGAAGC+AGG | + | chr5.2:58657002-58657021 | MS.gene44844:CDS | 35.0% | |
| AGAATACTATGGCCATGATT+GGG | + | chr5.2:58656146-58656165 | MS.gene44844:CDS | 35.0% | |
| AGACAGAATCTGTTGTTCAA+AGG | + | chr5.2:58657274-58657293 | MS.gene44844:CDS | 35.0% | |
| AGTAAGTGGCAACAAAAAGA+TGG | + | chr5.2:58657120-58657139 | MS.gene44844:CDS | 35.0% | |
| ATCTTTCTTTGATCCTCTGA+AGG | + | chr5.2:58657530-58657549 | MS.gene44844:CDS | 35.0% | |
| CAACGAGTATAAGGAACTTT+GGG | + | chr5.2:58657575-58657594 | MS.gene44844:CDS | 35.0% | |
| CAATAGCTTCAAGGTTAAAC+TGG | + | chr5.2:58657346-58657365 | MS.gene44844:CDS | 35.0% | |
| GAATGATAGTACCTGTGATA+TGG | + | chr5.2:58656567-58656586 | MS.gene44844:CDS | 35.0% | |
| GATAACAGTGTTGTGAAAAC+TGG | + | chr5.2:58656313-58656332 | MS.gene44844:CDS | 35.0% | |
| GATTTCCATATCAAATGCCA+TGG | - | chr5.2:58657300-58657319 | None:intergenic | 35.0% | |
| GTAAGTGGCAACAAAAAGAT+GGG | + | chr5.2:58657121-58657140 | MS.gene44844:CDS | 35.0% | |
| GTATTCTGACCTAAATTCTC+TGG | - | chr5.2:58656133-58656152 | None:intergenic | 35.0% | |
| GTTCATTTGTAGCAATGTAG+AGG | - | chr5.2:58657503-58657522 | None:intergenic | 35.0% | |
| GTTGAATACTTATCCTTCAG+AGG | - | chr5.2:58657546-58657565 | None:intergenic | 35.0% | |
| TAAATTTGGGAAACCTGTTC+CGG | + | chr5.2:58656540-58656559 | MS.gene44844:CDS | 35.0% | |
| TCAACGAGTATAAGGAACTT+TGG | + | chr5.2:58657574-58657593 | MS.gene44844:CDS | 35.0% | |
| TCAGGGTTCTTACAATCAAT+AGG | - | chr5.2:58656463-58656482 | None:intergenic | 35.0% | |
| TCGGAAAGTTCATTTAGTCA+AGG | + | chr5.2:58656841-58656860 | MS.gene44844:CDS | 35.0% | |
| TTCACAAATTCTCCAACAAC+CGG | - | chr5.2:58656799-58656818 | None:intergenic | 35.0% | |
| ! | ATGAGGAAGGCAAAGATTTT+AGG | + | chr5.2:58657025-58657044 | MS.gene44844:CDS | 35.0% |
| ! | CGGCGTTTTATAAGGATTAT+AGG | + | chr5.2:58656623-58656642 | MS.gene44844:CDS | 35.0% |
| ! | CGTTTAGTGTTGTTAGTATG+GGG | + | chr5.2:58656677-58656696 | MS.gene44844:CDS | 35.0% |
| ! | GAAAACTGGTGAGGATTTTA+TGG | + | chr5.2:58656327-58656346 | MS.gene44844:CDS | 35.0% |
| ! | GTTTAGTGTTGTTAGTATGG+GGG | + | chr5.2:58656678-58656697 | MS.gene44844:CDS | 35.0% |
| ! | TGATTTTGAGCATTTGAAGG+AGG | + | chr5.2:58657056-58657075 | MS.gene44844:CDS | 35.0% |
| !!! | GATACAGAAGTGTTTTTGAG+AGG | + | chr5.2:58657684-58657703 | MS.gene44844:CDS | 35.0% |
| AAAGGCCATGGCATTTGATA+TGG | + | chr5.2:58657292-58657311 | MS.gene44844:CDS | 40.0% | |
| AAATTTGGGAAACCTGTTCC+GGG | + | chr5.2:58656541-58656560 | MS.gene44844:CDS | 40.0% | |
| AACTTTCCGACTCAACTACA+GGG | - | chr5.2:58656831-58656850 | None:intergenic | 40.0% | |
| AATCTGTTGTTCAAAGGCCA+TGG | + | chr5.2:58657280-58657299 | MS.gene44844:CDS | 40.0% | |
| ACTCAGTTCATGTTGTGAGA+GGG | + | chr5.2:58657376-58657395 | MS.gene44844:CDS | 40.0% | |
| AGATGGGATGAATCTTCATC+TGG | + | chr5.2:58657137-58657156 | MS.gene44844:CDS | 40.0% | |
| AGTATGGGGGATTATCATAC+TGG | + | chr5.2:58656691-58656710 | MS.gene44844:CDS | 40.0% | |
| ATCATACTGGTATGAATGCG+AGG | + | chr5.2:58656704-58656723 | MS.gene44844:CDS | 40.0% | |
| ATGCACTTCTCTCAACCTTA+CGG | + | chr5.2:58657454-58657473 | MS.gene44844:CDS | 40.0% | |
| ATGTCGAGCCTGATAACTAT+TGG | + | chr5.2:58657232-58657251 | MS.gene44844:CDS | 40.0% | |
| CAGAATACTATGGCCATGAT+TGG | + | chr5.2:58656145-58656164 | MS.gene44844:CDS | 40.0% | |
| CCATAACCAATGTTCGGTTT+AGG | - | chr5.2:58656960-58656979 | None:intergenic | 40.0% | |
| GAAGAGGAAGAAGAATCAGA+AGG | + | chr5.2:58656726-58656745 | MS.gene44844:CDS | 40.0% | |
| GAGGTATGTGCATCAAGATT+TGG | - | chr5.2:58657429-58657448 | None:intergenic | 40.0% | |
| GCTACTAATGAGTCTGTGTT+TGG | + | chr5.2:58656349-58656368 | MS.gene44844:CDS | 40.0% | |
| GGATCAAAGAAAGATGCATC+AGG | - | chr5.2:58657525-58657544 | None:intergenic | 40.0% | |
| GGATGAAACAAAGGATCTTC+AGG | - | chr5.2:58656238-58656257 | None:intergenic | 40.0% | |
| GGCATTTGATATGGAAATCG+AGG | + | chr5.2:58657301-58657320 | MS.gene44844:CDS | 40.0% | |
| GGTCACACCAATGAAACTTA+TGG | + | chr5.2:58657173-58657192 | MS.gene44844:CDS | 40.0% | |
| GGTTTCAGACCAGAGAATTT+AGG | + | chr5.2:58656121-58656140 | MS.gene44844:CDS | 40.0% | |
| TAGTGTCTGCAATAGCTTCA+AGG | + | chr5.2:58657337-58657356 | MS.gene44844:CDS | 40.0% | |
| TATTGGTATAGAGTCTGCGA+AGG | + | chr5.2:58657249-58657268 | MS.gene44844:CDS | 40.0% | |
| TCAGTACCTAAACCGAACAT+TGG | + | chr5.2:58656951-58656970 | MS.gene44844:CDS | 40.0% | |
| TGATTTGACCAGTGATTGCA+AGG | + | chr5.2:58657731-58657750 | MS.gene44844:CDS | 40.0% | |
| ! | CCTAAACCGAACATTGGTTA+TGG | + | chr5.2:58656957-58656976 | MS.gene44844:CDS | 40.0% |
| ! | GTTGGACAAGGATCAGTTTT+GGG | + | chr5.2:58657089-58657108 | MS.gene44844:CDS | 40.0% |
| ! | TAAGGTTGAGAGAAGTGCAT+CGG | - | chr5.2:58657454-58657473 | None:intergenic | 40.0% |
| ! | TGTTGGACAAGGATCAGTTT+TGG | + | chr5.2:58657088-58657107 | MS.gene44844:CDS | 40.0% |
| ! | TTTGGGATGAGACTAGTGAA+TGG | + | chr5.2:58657592-58657611 | MS.gene44844:CDS | 40.0% |
| ! | TTTTGAGCATTTGAAGGAGG+CGG | + | chr5.2:58657059-58657078 | MS.gene44844:CDS | 40.0% |
| !! | GATGTTGATAACTCGGTTAG+CGG | + | chr5.2:58656391-58656410 | MS.gene44844:CDS | 40.0% |
| AGAGAAGACATCGTCGAATC+TGG | + | chr5.2:58656756-58656775 | MS.gene44844:CDS | 45.0% | |
| AGGAAGAAGAATCAGAAGGC+TGG | + | chr5.2:58656730-58656749 | MS.gene44844:CDS | 45.0% | |
| ATAGTACCTGTGATATGGCG+TGG | + | chr5.2:58656572-58656591 | MS.gene44844:CDS | 45.0% | |
| ATGGAAATCGAGGCGATTGA+TGG | + | chr5.2:58657311-58657330 | MS.gene44844:CDS | 45.0% | |
| CAGGTACTATCATTCGAACC+CGG | - | chr5.2:58656562-58656581 | None:intergenic | 45.0% | |
| CAGTGTTGTGAAAACTGGTG+AGG | + | chr5.2:58656318-58656337 | MS.gene44844:CDS | 45.0% | |
| CTCAGTTCATGTTGTGAGAG+GGG | + | chr5.2:58657377-58657396 | MS.gene44844:CDS | 45.0% | |
| GAACTTTCCGACTCAACTAC+AGG | - | chr5.2:58656832-58656851 | None:intergenic | 45.0% | |
| GACTCAGTTCATGTTGTGAG+AGG | + | chr5.2:58657375-58657394 | MS.gene44844:CDS | 45.0% | |
| GATACCATCCTTGCAATCAC+TGG | - | chr5.2:58657742-58657761 | None:intergenic | 45.0% | |
| GATGAATCTTCATCTGGTCG+AGG | + | chr5.2:58657143-58657162 | MS.gene44844:CDS | 45.0% | |
| GGCGTTCCAGTAGAATTTGA+TGG | + | chr5.2:58657642-58657661 | MS.gene44844:CDS | 45.0% | |
| TACTTGGTTTATGTGGGTGG+TGG | + | chr5.2:58656868-58656887 | MS.gene44844:CDS | 45.0% | |
| TCATCTGGTCGAGGATTACA+GGG | + | chr5.2:58657152-58657171 | MS.gene44844:CDS | 45.0% | |
| TCATGTTGTGAGAGGGGAAA+AGG | + | chr5.2:58657383-58657402 | MS.gene44844:CDS | 45.0% | |
| TGCACTTCTCTCAACCTTAC+GGG | + | chr5.2:58657455-58657474 | MS.gene44844:CDS | 45.0% | |
| TGGAGTTTCTTGTGTGCTCT+AGG | + | chr5.2:58656922-58656941 | MS.gene44844:CDS | 45.0% | |
| TTCATCTGGTCGAGGATTAC+AGG | + | chr5.2:58657151-58657170 | MS.gene44844:CDS | 45.0% | |
| TTGACCAGTGATTGCAAGGA+TGG | + | chr5.2:58657735-58657754 | MS.gene44844:CDS | 45.0% | |
| TTTGGGCAGATTGGAGTAAG+TGG | + | chr5.2:58657106-58657125 | MS.gene44844:CDS | 45.0% | |
| ! | AAGGTTGAGAGAAGTGCATC+GGG | - | chr5.2:58657453-58657472 | None:intergenic | 45.0% |
| ! | TCTGCATTTTCTAGACCTGG+AGG | + | chr5.2:58656100-58656119 | MS.gene44844:CDS | 45.0% |
| !! | TTTATGTGGGTGGTGGAGAT+AGG | + | chr5.2:58656875-58656894 | MS.gene44844:CDS | 45.0% |
| !!! | AGGATCAGTTTTGGGCAGAT+TGG | + | chr5.2:58657097-58657116 | MS.gene44844:CDS | 45.0% |
| ACTCAGTGTGAGGGGAATTC+AGG | + | chr5.2:58656436-58656455 | MS.gene44844:CDS | 50.0% | |
| AGGCGAGGAACAAAGAGCTT+TGG | + | chr5.2:58657403-58657422 | MS.gene44844:CDS | 50.0% | |
| ATACCTCCACGCCATATCAC+AGG | - | chr5.2:58656581-58656600 | None:intergenic | 50.0% | |
| CACTTCATCGAAGCAGGATG+AGG | + | chr5.2:58657008-58657027 | MS.gene44844:CDS | 50.0% | |
| CTATCATTCGAACCCGGAAC+AGG | - | chr5.2:58656556-58656575 | None:intergenic | 50.0% | |
| CTGGTATGAATGCGAGGAAG+AGG | + | chr5.2:58656710-58656729 | MS.gene44844:CDS | 50.0% | |
| GACATCGTCGAATCTGGATC+AGG | + | chr5.2:58656762-58656781 | MS.gene44844:CDS | 50.0% | |
| GGTTAGCGGTGAGACTAATG+AGG | + | chr5.2:58656405-58656424 | MS.gene44844:CDS | 50.0% | |
| TCATCGAAGCAGGATGAGGA+AGG | + | chr5.2:58657012-58657031 | MS.gene44844:CDS | 50.0% | |
| TCTCTCAACCTTACGGGACA+AGG | + | chr5.2:58657461-58657480 | MS.gene44844:CDS | 50.0% | |
| TTACGGGACAAGGTTGACGA+AGG | + | chr5.2:58657471-58657490 | MS.gene44844:CDS | 50.0% | |
| TTCGTCAACCTTGTCCCGTA+AGG | - | chr5.2:58657472-58657491 | None:intergenic | 50.0% | |
| TTTCCGACTCAACTACAGGG+AGG | - | chr5.2:58656828-58656847 | None:intergenic | 50.0% | |
| ! | AGGTTGAGAGAAGTGCATCG+GGG | - | chr5.2:58657452-58657471 | None:intergenic | 50.0% |
| ! | CGGTCTGCATTTTCTAGACC+TGG | + | chr5.2:58656097-58656116 | MS.gene44844:CDS | 50.0% |
| ! | GAAGGCTGCGGCGTTTTATA+AGG | + | chr5.2:58656615-58656634 | MS.gene44844:CDS | 50.0% |
| ! | GTGATATGGCGTGGAGGTAT+AGG | + | chr5.2:58656581-58656600 | MS.gene44844:CDS | 50.0% |
| ! | TTCTCTGGTCTGAAACCTCC+AGG | - | chr5.2:58656118-58656137 | None:intergenic | 50.0% |
| CAGCCTCCCTGTAGTTGAGT+CGG | + | chr5.2:58656822-58656841 | MS.gene44844:CDS | 55.0% | |
| CGGTGAGACTAATGAGGCTG+AGG | + | chr5.2:58656411-58656430 | MS.gene44844:CDS | 55.0% | |
| CTGAGGTGACTCAGTGTGAG+GGG | + | chr5.2:58656428-58656447 | MS.gene44844:CDS | 55.0% | |
| GCTGAGGTGACTCAGTGTGA+GGG | + | chr5.2:58656427-58656446 | MS.gene44844:CDS | 55.0% | |
| GGATCAGGTCAATGCGTTGC+CGG | + | chr5.2:58656777-58656796 | MS.gene44844:CDS | 55.0% | |
| GTACCTGTGATATGGCGTGG+AGG | + | chr5.2:58656575-58656594 | MS.gene44844:CDS | 55.0% | |
| GTATAGGCCGAAGGATGGGA+AGG | + | chr5.2:58656597-58656616 | MS.gene44844:CDS | 55.0% | |
| TTGTGAGAGGGGAAAAGGCG+AGG | + | chr5.2:58657388-58657407 | MS.gene44844:CDS | 55.0% | |
| ! | GGAGGTATAGGCCGAAGGAT+GGG | + | chr5.2:58656593-58656612 | MS.gene44844:CDS | 55.0% |
| ! | GGTTGAGAGAAGTGCATCGG+GGG | - | chr5.2:58657451-58657470 | None:intergenic | 55.0% |
| ! | GTCAATGCGTTGCCGGTTGT+TGG | + | chr5.2:58656784-58656803 | MS.gene44844:CDS | 55.0% |
| ! | TGGAGGTATAGGCCGAAGGA+TGG | + | chr5.2:58656592-58656611 | MS.gene44844:CDS | 55.0% |
| GGCTGAGGTGACTCAGTGTG+AGG | + | chr5.2:58656426-58656445 | MS.gene44844:CDS | 60.0% | |
| ! | TGAGAGAAGTGCATCGGGGG+AGG | - | chr5.2:58657448-58657467 | None:intergenic | 60.0% |
| GAAGGAGGCGGCGTCTGTGT+TGG | + | chr5.2:58657071-58657090 | MS.gene44844:CDS | 65.0% | |
| GCCGAAGGATGGGAAGGCTG+CGG | + | chr5.2:58656603-58656622 | MS.gene44844:CDS | 65.0% | |
| GCCGCAGCCTTCCCATCCTT+CGG | - | chr5.2:58656607-58656626 | None:intergenic | 65.0% | |
| GGCGGCGTCTGTGTTGGACA+AGG | + | chr5.2:58657077-58657096 | MS.gene44844:CDS | 65.0% | |
| ! | GGCGTGGAGGTATAGGCCGA+AGG | + | chr5.2:58656588-58656607 | MS.gene44844:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 58656088 | 58657788 | 58656088 | ID=MS.gene44844 |
| chr5.2 | mRNA | 58656088 | 58657788 | 58656088 | ID=MS.gene44844.t1;Parent=MS.gene44844 |
| chr5.2 | exon | 58656088 | 58657788 | 58656088 | ID=MS.gene44844.t1.exon1;Parent=MS.gene44844.t1 |
| chr5.2 | CDS | 58656088 | 58657788 | 58656088 | ID=cds.MS.gene44844.t1;Parent=MS.gene44844.t1 |
| Gene Sequence |
| Protein sequence |
