AlfalfaGEDB | MS.gene50615

M. sativa cultivar XinJiangDaYe / MS.gene50615

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF13176.9 PF13181.9 PF13374.9 PF13424.9 PF14559.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50615.t1	XP_013468521.1	97.4	547	14	0	2	548	48	594	5.80E-278	966.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50615.t1	Q9LII8	30.4	477	315	8	68	532	168	639	4.8e-47	190.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50615.t1	A0A072VMA4	97.4	547	14	0	2	548	48	594	4.2e-278	966.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049207	MS.gene50615	0.831504	1.61E-55	-1.69E-46
MS.gene049254	MS.gene50615	0.803414	3.53E-49	-1.69E-46
MS.gene049283	MS.gene50615	0.80133	9.48E-49	-1.69E-46
MS.gene049666	MS.gene50615	0.819872	9.25E-53	-1.69E-46
MS.gene049824	MS.gene50615	0.853962	1.69E-61	-1.69E-46
MS.gene049825	MS.gene50615	0.848846	4.72E-60	-1.69E-46
MS.gene049863	MS.gene50615	0.810502	1.12E-50	-1.69E-46
MS.gene049873	MS.gene50615	0.842118	3.14E-58	-1.69E-46
MS.gene050303	MS.gene50615	0.814273	1.68E-51	-1.69E-46
MS.gene050885	MS.gene50615	0.831134	1.98E-55	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50615.t1	MTR_1g070145	97.340	564	15	0	1	564	47	610	0.0	1125
MS.gene50615.t1	MTR_8g099745	28.715	498	330	6	45	528	65	551	2.88e-49	180
MS.gene50615.t1	MTR_8g027385	28.024	496	336	8	45	529	52	537	2.25e-44	166
MS.gene50615.t1	MTR_3g077950	26.326	509	359	6	37	536	273	774	1.22e-42	164
MS.gene50615.t1	MTR_3g077950	26.326	509	359	6	37	536	245	746	1.30e-42	164
MS.gene50615.t1	MTR_6g007110	27.879	495	324	7	52	528	190	669	2.67e-41	159
MS.gene50615.t1	MTR_4g106900	25.255	491	355	5	45	528	167	652	7.64e-41	157
MS.gene50615.t1	MTR_6g007110	27.974	454	294	7	52	487	190	628	8.25e-38	148

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50615.t1	AT2G31240	68.556	547	166	4	1	544	68	611	0.0	772
MS.gene50615.t1	AT3G27960	30.171	527	346	11	45	557	143	661	4.39e-53	192
MS.gene50615.t1	AT3G27960	30.171	527	346	11	45	557	143	661	4.39e-53	192
MS.gene50615.t1	AT3G27960	30.044	456	301	8	45	487	143	593	2.72e-48	177
MS.gene50615.t1	AT4G10840	29.191	507	324	9	47	529	78	573	7.42e-48	177
MS.gene50615.t1	AT4G10840	28.879	464	296	8	47	487	78	530	6.28e-44	164
MS.gene50615.t1	AT1G27500	26.793	474	334	5	68	533	161	629	7.52e-42	160
MS.gene50615.t1	AT1G27500	26.653	484	322	7	68	533	172	640	8.64e-42	160

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Find 143 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTTGCAGACTGTAAA+AGG	0.282943	1.2:-53499914	MS.gene50615:CDS
AACATCTGCGCTGCTGACTG+AGG	0.607323	1.2:+53499534	None:intergenic
AACTGCAAACTCATAGCATA+AGG	0.469559	1.2:+53500593	None:intergenic
AAGAGAACAGATTACACCGG+TGG	0.642489	1.2:-53499859	MS.gene50615:CDS
AAGAGGCGCTAGAGAATCTT+CGG	0.442376	1.2:-53500391	MS.gene50615:CDS
AATAGGTTTAGTGATAGTTT+AGG	0.302645	1.2:-53500545	MS.gene50615:CDS
AATTGCACAGCATGAAGCAC+AGG	0.576090	1.2:+53500452	None:intergenic
ACGTGGCGGTGTGTCAGGTT+GGG	0.514066	1.2:+53497848	None:intergenic
ACTGTTCGGGATTGACGTGG+CGG	0.661175	1.2:+53497834	None:intergenic
AGAAACCTCCATGATATCCT+TGG	0.489502	1.2:+53499503	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGTTTGCAGACTGTAAA+AGG	-	chr1.2:53498788-53498807	MS.gene50615:intron	30.0%
AAACCAAGAGAAACAATATC+AGG	+	chr1.2:53499328-53499347	None:intergenic	30.0%
AACCAAGAGAAACAATATCA+GGG	+	chr1.2:53499327-53499346	None:intergenic	30.0%
AAGAAAAATTAGGCTGTGTT+TGG	-	chr1.2:53499587-53499606	MS.gene50615:CDS	30.0%
AAGCACATTATCCAAAAACA+AGG	+	chr1.2:53500476-53500495	None:intergenic	30.0%
AGCACATTATCCAAAAACAA+GGG	+	chr1.2:53500475-53500494	None:intergenic	30.0%
AGGTGTTTAGAGATTTCTAT+TGG	-	chr1.2:53498808-53498827	MS.gene50615:intron	30.0%
ATAACCAACTCCAAAATGTT+TGG	+	chr1.2:53499106-53499125	None:intergenic	30.0%
ATCTTGAGTACAAAGAGTTA+TGG	-	chr1.2:53500100-53500119	MS.gene50615:CDS	30.0%
CTATTATGGGATTGATGTAA+CGG	+	chr1.2:53500811-53500830	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	53497830	53500894	53497830	ID=MS.gene50615
chr1.2	mRNA	53497830	53500894	53497830	ID=MS.gene50615.t1;Parent=MS.gene50615
chr1.2	exon	53499432	53500894	53499432	ID=MS.gene50615.t1.exon1;Parent=MS.gene50615.t1
chr1.2	CDS	53499432	53500894	53499432	ID=cds.MS.gene50615.t1;Parent=MS.gene50615.t1
chr1.2	exon	53497830	53498130	53497830	ID=MS.gene50615.t1.exon2;Parent=MS.gene50615.t1
chr1.2	CDS	53497830	53498130	53497830	ID=cds.MS.gene50615.t1;Parent=MS.gene50615.t1

Gene Sequence

>MS.gene50615

ATGTCAACCCTCATCCAAAAACCCACTCAAATCTCTCCTAGACAACGCAAAATCATCGAAAAATCGCATCTTGAAGAAGCTTTTGAGTCCGCCAAAACAACCGAAGAAATGCTCAAAGCATTCAGTGAGATGGAAATTTCATTCAATGAAAAGGAGCTTGGTTTAGCTGCATTGAAAATTGGTCTGAAGCTTGATCGTGAAGGTGAGGATCCTGAAAAAGCTCTTGAATTTGCTAATAGAGCTTTGAAAGTGTTTGAAAATGAAAAGAAAGATTCTTTTCCTTATGCTATGAGTTTGCAGTTAATGGGTAGTCTTAATTATGGTTTAAATAGGTTTAGTGATAGTTTAGGGTATCTTAATAAGGCTAATAGGGTTTTGGGTAGGTTAGAGAATGAGGGTGTTTGTGTTGATGATGTTAGGCCTGTGCTTCATGCTGTGCAATTTGAGTTGGCTAATGTTAAGACGGCGATGGGGAGGAGAGAAGAGGCGCTAGAGAATCTTCGGAAGTGTTTGGAGATTAAGGAGATGACTTTTGAGGAAGATAGTGAGGAGTTAGGGAAAGGGAATAGGGATTTAGCGGAAGCTTATGTTGCGGTTTTGAATTTTAAGGAGGCGCTTCCGTATTGTTTAAAGGCGTTAGAGATACATACGAAGCGATTAGGGATGAATTCTGTTGAGGTTGCGCATGATAGGAAGCTTCTTGGGATTGTTTATAGTGGGATGGAGGATCATGAGAAGGCGTTGGAGCAGAATGTTTTGGCGCAGAAGATTTTGAAGAATTGGAATCTCAGTTCGGATTTGTTGCGCGCTGAAGTTGATGCTGCGAATATGATGATTGCTTTGGGGAGATACGATGAAGCTGTTGGTACTTTGAAGAACGTTGTGAATCAGACTGAGAAAGATAGTGAGACTCGAGCTCTTGTGCTTGTATCAATGGGGAAAGCGTTATGTAATCAGGAAAAGTTTGCAGACTGTAAAAGGTGTTTAGAGATTTCTATTGGGATTCTTGATAAAAGAGAACAGATTACACCGGTGGAAGTTTCTGAGGCATACTCGGAGATATCCATGCTATATGAAACTATGAACGAGTTCGAAACTGCGATTTCGTTGCTGAAGAGGGCACTAGCTTTGCTCGAGAAGCTCCCACAAGAGCAGCACTCGGAGGGAAGTGTATCTGCAAGAATAGGGTGGTTGTTGTTGCTTACAGGTAAGGTGAAGCAGGCTATTCCTTATTTGGAAAGTGCTGCTGAACGGTTGAAAGAGAGCTTTGGTCCCAAACATTTTGGAGTTGGTTATATCTACAACAATTTGGGGGCAGCATATTTAGAGTTGGATAGACCTCAGTCAGCAGCGCAGATGTTTGCGGTAGCCAAGGATATCATGGAGGTTTCTCTTGGTCCACATCATGCAGATACAATTGAAGCATGTCAGAATCTTTCAAAGGCTTATGGTGATATTGGAAGGTATGGATTGTTTTATTGCTTTGACTTTATTCCCTGATATTGTTTCTCTTGGTTTGTTGATTCTGCAAACAATTGGTTTTGGGTACACTTTACTCTAGCAGTTTAAACATTTAACTTTTGGACATATACCTAATAGTGTGGGATTCAGATTCCAGTGGAACAATTATTTTGAGACTTAAGGATGGCTGAATTCTCTTGCATTCAAGTGTAACGATTTCTGACATAAAATAGAGTGCAGGGTGTACTTAATACGTCTGCTTCTATTTCTTCATTGTGTTGATTGACTGCTTTCAAAAGAAAAATTAGGCTGTGTTTGGATCAACAACTTAATTAAGCGATTATCATATAAGTACTTTATGTGTAAGCTTTTTCTATAACAAAAGATAAAATAAAGTTAAACTATTTTCATATAAGTAGCTATGTTGGAGAACTTATTGAAATAAGCTGAGAACATTTTATGAACATGTCATAAGCTGTTTATGTAAGCTCTCCAAACACTGTTGCTAGTTCTTATGCTAGTAGATAACCTCAAATAAGCCATTCCAAACAGGTCCTTATTCATGCTATGCTCATAATCCTTTTAGTTTCCATGTCTACCTCTCATATAGTTCTTTCCATCTATCAAACTAAGTTTTATTGCTTTCTAAGAGTGAGTTAATTGTTTAACCACTATATTCTCTTCTGTGCTCACCAAGAGAGTTCTGATTTGGTGTTTTGTGTTGTACACTGTAAATTTCTAGTCTTCACATATTAGATAAAATAAAGTGTATCTGAGTAGAATTTGAATGATGAAATTCTCGAGAGTAGATCTTGAGTACAAAGAGTTATGGCCTGAGCCCATAATTGCACATGGAATTGCTGCTAATGTAGTCACTAGTCAGACATCAATGTTTTGCTTGTTTTTGGAACTTTATGCAAACTTATTTCATTATTTAGATCGGTCCATTTCGAGCTCAATTGCATATGATTATTTTGTTTGCAGTCTACACAAATTTTATTTATGAAGTTTATGCACACATGATTTTTTTTCCTATATAGTATGTACTTGAATTACAAAAATATTTAGGAATCGGAAGGTACAATTTAGACTTGGTTGTTCCCGGTTCAGAGGTTCTCGTAAAGGAATTTAATGAATTCAACGTAACATACTGTGAGTGGAATGTGCAAACTTGTTTCTGTCCCTTGTTTTTGGATAATGTGCTTCTATTTTCACTAGTTTAATTTAATTGTCATTGCATTGTGAACTGTATATGTTTTTCCAACGGAAAAATTCATGTTTTCCTAAAATATTGGAATTGCAGCCATTCCCTTGCAATTGAGTTTCAGCAGCAAGTTATAGATGCTTGGGAAAGCCATGGAGCAAGTGCAGAAGAAGAACTAAGAGAAGCTCAGCGACTACTCGAACAATTAAAGAAAAAAGCCCGCGGTGTCTCTGCAAATGAGGTTCCTATGAAGGCTCTTCCCTTTCCTAATAATAATACACATGCAGCATCCAATAACTCCCAACCTGACATACCGTTACATCAATCCCATAATAGTACACCTGCAGCATCCAGTAACTCCCAACCTGACACACCGCCACGTCAATCCCGAACAGTTTAG

Protein sequence

>MS.gene50615.t1

MSTLIQKPTQISPRQRKIIEKSHLEEAFESAKTTEEMLKAFSEMEISFNEKELGLAALKIGLKLDREGEDPEKALEFANRALKVFENEKKDSFPYAMSLQLMGSLNYGLNRFSDSLGYLNKANRVLGRLENEGVCVDDVRPVLHAVQFELANVKTAMGRREEALENLRKCLEIKEMTFEEDSEELGKGNRDLAEAYVAVLNFKEALPYCLKALEIHTKRLGMNSVEVAHDRKLLGIVYSGMEDHEKALEQNVLAQKILKNWNLSSDLLRAEVDAANMMIALGRYDEAVGTLKNVVNQTEKDSETRALVLVSMGKALCNQEKFADCKRCLEISIGILDKREQITPVEVSEAYSEISMLYETMNEFETAISLLKRALALLEKLPQEQHSEGSVSARIGWLLLLTGKVKQAIPYLESAAERLKESFGPKHFGVGYIYNNLGAAYLELDRPQSAAQMFAVAKDIMEVSLGPHHADTIEACQNLSKAYGDIGSHSLAIEFQQQVIDAWESHGASAEEELREAQRLLEQLKKKARGVSANEVPMKALPFPNNNTHAASNNSQPDIPLHQSHNSTPAASSNSQPDTPPRQSRTV