AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51964


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51964.t1 MTR_3g030055 79.518 166 23 2 1 166 1 155 2.97e-89 257
MS.gene51964.t1 MTR_3g030055 80.769 104 12 1 8 111 1 96 1.22e-54 169
MS.gene51964.t1 MTR_3g034200 47.239 163 67 6 13 164 2 156 2.16e-32 114
MS.gene51964.t1 MTR_3g050860 41.463 123 63 4 44 164 37 152 7.19e-16 73.9
MS.gene51964.t1 MTR_0439s0020 32.331 133 76 5 8 136 1 123 4.07e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51964.t1 AT5G13780 44.444 162 71 6 13 163 2 155 1.06e-28 105

Find 40 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCACGATCTGCCAATATT+CGG 0.311860 3.2:-4180580 None:intergenic
TGTTCCTTGTAACTGTTTCA+TGG 0.332180 3.2:-4180473 None:intergenic
TCCACGATCTGCCAATATTC+GGG 0.342849 3.2:-4180579 None:intergenic
GAGCTGCAGAGTTGCTCTTC+CGG 0.345662 3.2:-4181485 None:intergenic
TCTTGGTTTGTTTGAAAAGA+TGG 0.363394 3.2:+4181506 MS.gene51964:CDS
AGAGGAAGTGGAAGAGGAAG+AGG 0.396334 3.2:+4180663 MS.gene51964:CDS
CTCGTTACTCTCCCGAATAT+TGG 0.398957 3.2:+4180568 MS.gene51964:CDS
AGTGGAAGAGGAAGAGGAAG+AGG 0.420722 3.2:+4180669 MS.gene51964:CDS
AACAATACAGCCGCTGCAAT+CGG 0.422612 3.2:-4180623 None:intergenic
AGAGGAAGAGGAAGAGGAAG+AGG 0.426997 3.2:+4180675 MS.gene51964:CDS
CTCCTCATCATCAAAATACT+TGG 0.430558 3.2:-4181553 None:intergenic
TGCATAAGAGTCGCAACAAT+TGG 0.433948 3.2:+4180514 MS.gene51964:CDS
CTCTAAAGAGGAAGTGGAAG+AGG 0.438354 3.2:+4180657 MS.gene51964:CDS
GAAGAGGAAGAGGAAGAGGA+AGG 0.438355 3.2:+4180679 MS.gene51964:CDS
CGCCCAAAACACCATGGAAA+AGG 0.453353 3.2:+4180783 MS.gene51964:CDS
CTTGGTTTGTTTGAAAAGAT+GGG 0.459057 3.2:+4181507 MS.gene51964:CDS
CCTCCTCTCTAAAGAGGAAG+TGG 0.459131 3.2:+4180651 MS.gene51964:CDS
AAGAGCAACTCTGCAGCTCT+TGG 0.462439 3.2:+4181489 MS.gene51964:CDS
TGCTTACCTCCTCTCTAAAG+AGG 0.467882 3.2:+4180645 MS.gene51964:CDS
CAATTGGAGAAGTATTAGAT+TGG 0.473450 3.2:+4180530 MS.gene51964:CDS
AAGATGTCTCGGTGCACGTC+CGG 0.505757 3.2:+4181466 MS.gene51964:CDS
CTTCCACTTCCTCTTTAGAG+AGG 0.515368 3.2:-4180654 None:intergenic
GAAGAGGAAGAGGAAGGGAA+TGG 0.522153 3.2:+4180685 MS.gene51964:CDS
AAGAGGAAGAGGAAGAGGAA+GGG 0.522871 3.2:+4180680 MS.gene51964:CDS
CCACTTCCTCTTTAGAGAGG+AGG 0.536526 3.2:-4180651 None:intergenic
GAGTGGCTCTCACAGTTAGG+TGG 0.542114 3.2:-4181619 None:intergenic
AGGAACAATGTCGTCAAAGA+AGG 0.558119 3.2:+4180489 MS.gene51964:CDS
CACGTCGCGGCCGATTGCAG+CGG 0.560201 3.2:+4180613 MS.gene51964:CDS
TTACACACTTCTTGAGGTGA+AGG 0.565558 3.2:+4181530 MS.gene51964:CDS
ATAGGTATGGTGAACAAAGA+GGG 0.575631 3.2:-4180725 None:intergenic
GATGGGTTACACACTTCTTG+AGG 0.581446 3.2:+4181524 MS.gene51964:CDS
AGGAGGACGCTATTGTCTTG+TGG 0.587701 3.2:+4181571 MS.gene51964:CDS
TCCCGAATATTGGCAGATCG+TGG 0.596909 3.2:+4180578 MS.gene51964:CDS
CTAACCATGAAACAGTTACA+AGG 0.600808 3.2:+4180469 None:intergenic
GATAGGTATGGTGAACAAAG+AGG 0.607709 3.2:-4180726 None:intergenic
CACCGCCGCCCAAAACACCA+TGG 0.620119 3.2:+4180777 MS.gene51964:CDS
CACCATACCTATCGTAAACG+TGG 0.628725 3.2:+4180736 MS.gene51964:CDS
GGTGGGTGCTAAAGATGTCT+CGG 0.629911 3.2:+4181455 MS.gene51964:intron
TACCACGTTTACGATAGGTA+TGG 0.651983 3.2:-4180738 None:intergenic
AGCGATACCACGTTTACGAT+AGG 0.681782 3.2:-4180743 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTGTTTTTTGGATT+AGG + chr3.2:4180830-4180849 MS.gene51964:intron 15.0%
!!! TCTGTTATAATTTTGTTTTT+TGG + chr3.2:4180824-4180843 MS.gene51964:intron 15.0%
! ACATTGTAACAGACTATTAA+TGG - chr3.2:4181421-4181440 None:intergenic 25.0%
! CAACCAAAAACATCAAAAAA+AGG - chr3.2:4181382-4181401 None:intergenic 25.0%
! GAAAACCAAAAGAAAAATCT+CGG - chr3.2:4181287-4181306 None:intergenic 25.0%
!! TTTCTTCTTGTTAGTGATTT+TGG + chr3.2:4181204-4181223 MS.gene51964:intron 25.0%
!!! AGACCTTTTTTTGATGTTTT+TGG + chr3.2:4181376-4181395 MS.gene51964:intron 25.0%
ACAATGTGTAATTTGACTGA+AGG + chr3.2:4181434-4181453 MS.gene51964:intron 30.0%
AGAAAACAGAAAATCATCAC+AGG - chr3.2:4181033-4181052 None:intergenic 30.0%
TCCATACATAACCAGTTAAA+AGG - chr3.2:4180895-4180914 None:intergenic 30.0%
! CTAGGATTATCTTTTATTCC+TGG + chr3.2:4181076-4181095 MS.gene51964:intron 30.0%
! TATGTATGGAGTTGGTTTAA+TGG + chr3.2:4180905-4180924 MS.gene51964:intron 30.0%
! TGTGATGATTTTCTGTTTTC+TGG + chr3.2:4181032-4181051 MS.gene51964:intron 30.0%
! TTATCTTTTATTCCTGGAAG+TGG + chr3.2:4181082-4181101 MS.gene51964:intron 30.0%
!! CAATTGGAGAAGTATTAGAT+TGG + chr3.2:4180530-4180549 MS.gene51964:CDS 30.0%
!! CTTGGTTTGTTTGAAAAGAT+GGG + chr3.2:4181507-4181526 MS.gene51964:CDS 30.0%
!! TCTTGGTTTGTTTGAAAAGA+TGG + chr3.2:4181506-4181525 MS.gene51964:CDS 30.0%
!! TTTTCTTGTTTCCTAGTCTT+GGG + chr3.2:4181302-4181321 MS.gene51964:intron 30.0%
AAAACACCATGGAAAAGGTT+AGG + chr3.2:4180788-4180807 MS.gene51964:intron 35.0%
AACAAAAAACCTAGCCTACT+CGG - chr3.2:4180976-4180995 None:intergenic 35.0%
AACCAAAAGAAAAATCTCGG+AGG - chr3.2:4181284-4181303 None:intergenic 35.0%
ATAGGTATGGTGAACAAAGA+GGG - chr3.2:4180728-4180747 None:intergenic 35.0%
ATGTGTAATTTGACTGAAGG+TGG + chr3.2:4181437-4181456 MS.gene51964:intron 35.0%
CTCCTCATCATCAAAATACT+TGG - chr3.2:4181556-4181575 None:intergenic 35.0%
TAACTGGTTATGTATGGAGT+TGG + chr3.2:4180897-4180916 MS.gene51964:intron 35.0%
TAATGGTTAGGTATGAAGCT+TGG + chr3.2:4180922-4180941 MS.gene51964:intron 35.0%
TGTGTAATTTGACTGAAGGT+GGG + chr3.2:4181438-4181457 MS.gene51964:intron 35.0%
! AACAAACCTAACCTTTTCCA+TGG - chr3.2:4180797-4180816 None:intergenic 35.0%
! AACCTTTTCCATGGTGTTTT+GGG - chr3.2:4180788-4180807 None:intergenic 35.0%
! ATGGAGTTGGTTTAATGGTT+AGG + chr3.2:4180910-4180929 MS.gene51964:intron 35.0%
! CTTCTTGTTAGTGATTTTGG+TGG + chr3.2:4181207-4181226 MS.gene51964:intron 35.0%
! TAACCTTTTCCATGGTGTTT+TGG - chr3.2:4180789-4180808 None:intergenic 35.0%
! TTTTCAGATAGATCACACAG+TGG - chr3.2:4181010-4181029 None:intergenic 35.0%
!! GCCTTTTAACTGGTTATGTA+TGG + chr3.2:4180891-4180910 MS.gene51964:intron 35.0%
!! GTTTTCTTGTTTCCTAGTCT+TGG + chr3.2:4181301-4181320 MS.gene51964:intron 35.0%
!! TTGTTAGTGATTTTGGTGGT+TGG + chr3.2:4181211-4181230 MS.gene51964:intron 35.0%
!! TTTCTTGTTTCCTAGTCTTG+GGG + chr3.2:4181303-4181322 MS.gene51964:intron 35.0%
!! TTTGTGTAGTTAGTCTTGTC+TGG + chr3.2:4181245-4181264 MS.gene51964:intron 35.0%
!!! CAAGTATTTTGATGATGAGG+AGG + chr3.2:4181554-4181573 MS.gene51964:CDS 35.0%
AAAATCCATACCCCAAGACT+AGG - chr3.2:4181316-4181335 None:intergenic 40.0%
AAACCAATTCTCTGTGGGAA+GGG + chr3.2:4181156-4181175 MS.gene51964:intron 40.0%
AAAGGGAGTGGCAATGATTA+GGG - chr3.2:4181122-4181141 None:intergenic 40.0%
AATCTGAGTTTCACTAGGCT+AGG + chr3.2:4181058-4181077 MS.gene51964:intron 40.0%
AGGAACAATGTCGTCAAAGA+AGG + chr3.2:4180489-4180508 MS.gene51964:CDS 40.0%
GATAGGTATGGTGAACAAAG+AGG - chr3.2:4180729-4180748 None:intergenic 40.0%
GGTAGAATCTGAGTTTCACT+AGG + chr3.2:4181053-4181072 MS.gene51964:intron 40.0%
GTTAGGTATGAAGCTTGGTT+CGG + chr3.2:4180927-4180946 MS.gene51964:intron 40.0%
TAAAGGGAGTGGCAATGATT+AGG - chr3.2:4181123-4181142 None:intergenic 40.0%
TACCACGTTTACGATAGGTA+TGG - chr3.2:4180741-4180760 None:intergenic 40.0%
TATTCCTGGAAGTGGTTCTT+TGG + chr3.2:4181090-4181109 MS.gene51964:intron 40.0%
TGCATAAGAGTCGCAACAAT+TGG + chr3.2:4180514-4180533 MS.gene51964:CDS 40.0%
TTACACACTTCTTGAGGTGA+AGG + chr3.2:4181530-4181549 MS.gene51964:CDS 40.0%
TTCCACGATCTGCCAATATT+CGG - chr3.2:4180583-4180602 None:intergenic 40.0%
! GGATTAGGTTTGTCACTTGT+GGG + chr3.2:4180845-4180864 MS.gene51964:intron 40.0%
! GTCCTCCGAGATTTTTCTTT+TGG + chr3.2:4181279-4181298 MS.gene51964:intron 40.0%
! TGGATTAGGTTTGTCACTTG+TGG + chr3.2:4180844-4180863 MS.gene51964:intron 40.0%
! TGTGGTATAGGCCTTTTAAC+TGG + chr3.2:4180881-4180900 MS.gene51964:intron 40.0%
! TTGGGGTATGGATTTTCTCT+AGG + chr3.2:4181320-4181339 MS.gene51964:intron 40.0%
!! TTGGTTCGGTTGTTTCGTTA+GGG + chr3.2:4180941-4180960 MS.gene51964:intron 40.0%
!!! GGCCAAGTATTTTGATGATG+AGG + chr3.2:4181551-4181570 MS.gene51964:CDS 40.0%
AAACCCTTCCCACAGAGAAT+TGG - chr3.2:4181162-4181181 None:intergenic 45.0%
AACAATACAGCCGCTGCAAT+CGG - chr3.2:4180626-4180645 None:intergenic 45.0%
AAGAGGAAGAGGAAGAGGAA+GGG + chr3.2:4180674-4180693 MS.gene51964:CDS 45.0%
AGCGATACCACGTTTACGAT+AGG - chr3.2:4180746-4180765 None:intergenic 45.0%
AGGCGAAACCAATTCTCTGT+GGG + chr3.2:4181151-4181170 MS.gene51964:intron 45.0%
ATTGCCACTCCCTTTATGAG+TGG + chr3.2:4181127-4181146 MS.gene51964:intron 45.0%
CACCATACCTATCGTAAACG+TGG + chr3.2:4180736-4180755 MS.gene51964:CDS 45.0%
CTCGTTACTCTCCCGAATAT+TGG + chr3.2:4180568-4180587 MS.gene51964:CDS 45.0%
GAAACCAATTCTCTGTGGGA+AGG + chr3.2:4181155-4181174 MS.gene51964:intron 45.0%
TAGCGTATAGGGTGTGGTAT+AGG + chr3.2:4180869-4180888 MS.gene51964:intron 45.0%
TCCACGATCTGCCAATATTC+GGG - chr3.2:4180582-4180601 None:intergenic 45.0%
TTCACCAAAGAACCACTTCC+AGG - chr3.2:4181097-4181116 None:intergenic 45.0%
TTGCCACTCCCTTTATGAGT+GGG + chr3.2:4181128-4181147 MS.gene51964:intron 45.0%
TTTCGCCTCCCACTCATAAA+GGG - chr3.2:4181139-4181158 None:intergenic 45.0%
! CTCTAAAGAGGAAGTGGAAG+AGG + chr3.2:4180657-4180676 MS.gene51964:CDS 45.0%
! CTTCCACTTCCTCTTTAGAG+AGG - chr3.2:4180657-4180676 None:intergenic 45.0%
! GAAAAAGCTTGTTCACGTCG+CGG + chr3.2:4180600-4180619 MS.gene51964:CDS 45.0%
! TGCTTACCTCCTCTCTAAAG+AGG + chr3.2:4180645-4180664 MS.gene51964:CDS 45.0%
! TTCTCTGTGGGAAGGGTTTT+TGG + chr3.2:4181163-4181182 MS.gene51964:intron 45.0%
!! CACTTGTGGGTTAGCGTATA+GGG + chr3.2:4180858-4180877 MS.gene51964:intron 45.0%
!! CTTGGTTCGGTTGTTTCGTT+AGG + chr3.2:4180940-4180959 MS.gene51964:intron 45.0%
!! CTTTTCCATGGTGTTTTGGG+CGG - chr3.2:4180785-4180804 None:intergenic 45.0%
!! GATGGGTTACACACTTCTTG+AGG + chr3.2:4181524-4181543 MS.gene51964:CDS 45.0%
!! TCACTTGTGGGTTAGCGTAT+AGG + chr3.2:4180857-4180876 MS.gene51964:intron 45.0%
!! TGGTTCGGTTGTTTCGTTAG+GGG + chr3.2:4180942-4180961 MS.gene51964:intron 45.0%
!! TGTTTCCTAGTCTTGGGGTA+TGG + chr3.2:4181308-4181327 MS.gene51964:intron 45.0%
AAGAGCAACTCTGCAGCTCT+TGG + chr3.2:4181489-4181508 MS.gene51964:CDS 50.0%
AAGGTCTGTACAACAGCTGC+AGG - chr3.2:4181363-4181382 None:intergenic 50.0%
AGAGGAAGAGGAAGAGGAAG+AGG + chr3.2:4180675-4180694 MS.gene51964:CDS 50.0%
AGAGGAAGTGGAAGAGGAAG+AGG + chr3.2:4180663-4180682 MS.gene51964:CDS 50.0%
AGGAGGACGCTATTGTCTTG+TGG + chr3.2:4181571-4181590 MS.gene51964:CDS 50.0%
AGTGGAAGAGGAAGAGGAAG+AGG + chr3.2:4180669-4180688 MS.gene51964:CDS 50.0%
CGCCCAAAACACCATGGAAA+AGG + chr3.2:4180783-4180802 MS.gene51964:CDS 50.0%
GAAGAGGAAGAGGAAGAGGA+AGG + chr3.2:4180673-4180692 MS.gene51964:CDS 50.0%
GAAGAGGAAGAGGAAGGGAA+TGG + chr3.2:4180685-4180704 MS.gene51964:CDS 50.0%
GAGGCGAAACCAATTCTCTG+TGG + chr3.2:4181150-4181169 MS.gene51964:intron 50.0%
GGTGGGTGCTAAAGATGTCT+CGG + chr3.2:4181455-4181474 MS.gene51964:intron 50.0%
GTCTTGTCTGGTGAGTGTTC+TGG + chr3.2:4181257-4181276 MS.gene51964:intron 50.0%
GTTTCGCCTCCCACTCATAA+AGG - chr3.2:4181140-4181159 None:intergenic 50.0%
TACAACAGCTGCAGGTCTCA+AGG - chr3.2:4181355-4181374 None:intergenic 50.0%
TCCCGAATATTGGCAGATCG+TGG + chr3.2:4180578-4180597 MS.gene51964:CDS 50.0%
TTGTTTCGTTAGGGGTGGGA+AGG + chr3.2:4180950-4180969 MS.gene51964:intron 50.0%
! CCACTTCCTCTTTAGAGAGG+AGG - chr3.2:4180654-4180673 None:intergenic 50.0%
! CCTCCTCTCTAAAGAGGAAG+TGG + chr3.2:4180651-4180670 MS.gene51964:CDS 50.0%
! TCGGTTGTTTCGTTAGGGGT+GGG + chr3.2:4180946-4180965 MS.gene51964:intron 50.0%
! TTCGGTTGTTTCGTTAGGGG+TGG + chr3.2:4180945-4180964 MS.gene51964:intron 50.0%
CCACTCCCTTTATGAGTGGG+AGG + chr3.2:4181131-4181150 MS.gene51964:intron 55.0%
CCTCCCACTCATAAAGGGAG+TGG - chr3.2:4181134-4181153 None:intergenic 55.0%
GAGCTGCAGAGTTGCTCTTC+CGG - chr3.2:4181488-4181507 None:intergenic 55.0%
! AAGATGTCTCGGTGCACGTC+CGG + chr3.2:4181466-4181485 MS.gene51964:CDS 55.0%
!! GTGGGTTAGCGTATAGGGTG+TGG + chr3.2:4180863-4180882 MS.gene51964:intron 55.0%
!! TTCCATGGTGTTTTGGGCGG+CGG - chr3.2:4180782-4180801 None:intergenic 55.0%
CACCGCCGCCCAAAACACCA+TGG + chr3.2:4180777-4180796 MS.gene51964:CDS 65.0%
GTGGGAAGGCCGAGTAGGCT+AGG + chr3.2:4180964-4180983 MS.gene51964:intron 65.0%
TAGGGGTGGGAAGGCCGAGT+AGG + chr3.2:4180959-4180978 MS.gene51964:intron 65.0%
CACGTCGCGGCCGATTGCAG+CGG + chr3.2:4180613-4180632 MS.gene51964:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 4180475 4181626 4180475 ID=MS.gene51964
chr3.2 mRNA 4180475 4181626 4180475 ID=MS.gene51964.t1;Parent=MS.gene51964
chr3.2 exon 4180475 4180804 4180475 ID=MS.gene51964.t1.exon1;Parent=MS.gene51964.t1
chr3.2 CDS 4180475 4180804 4180475 ID=cds.MS.gene51964.t1;Parent=MS.gene51964.t1
chr3.2 exon 4181456 4181626 4181456 ID=MS.gene51964.t1.exon2;Parent=MS.gene51964.t1
chr3.2 CDS 4181456 4181626 4181456 ID=cds.MS.gene51964.t1;Parent=MS.gene51964.t1
Gene Sequence

>MS.gene51964

ATGAAACAGTTACAAGGAACAATGTCGTCAAAGAAGGTGTGCATAAGAGTCGCAACAATTGGAGAAGTATTAGATTGGAACAGTAACAACAATCTCGTTACTCTCCCGAATATTGGCAGATCGTGGAAAAAGCTTGTTCACGTCGCGGCCGATTGCAGCGGCTGTATTGTTGCTTACCTCCTCTCTAAAGAGGAAGTGGAAGAGGAAGAGGAAGAGGAAGAGGAAGGGAATGGTCACTTTTGTATCGCTTCCCTCTTTGTTCACCATACCTATCGTAAACGTGGTATCGCTACTGATCTTATCACCGCCGCCCAAAACACCATGGAAAAGGTTAGGTTTGTTTCTTTATTCTGTTATAATTTTGTTTTTTGGATTAGGTTTGTCACTTGTGGGTTAGCGTATAGGGTGTGGTATAGGCCTTTTAACTGGTTATGTATGGAGTTGGTTTAATGGTTAGGTATGAAGCTTGGTTCGGTTGTTTCGTTAGGGGTGGGAAGGCCGAGTAGGCTAGGTTTTTTGTTAATCTAGCACGCCACTGTGTGATCTATCTGAAAACCTGTGATGATTTTCTGTTTTCTGGTAGAATCTGAGTTTCACTAGGCTAGGATTATCTTTTATTCCTGGAAGTGGTTCTTTGGTGAATTCCCTAATCATTGCCACTCCCTTTATGAGTGGGAGGCGAAACCAATTCTCTGTGGGAAGGGTTTTTGGCTTCTTTTCTTCTTTCTCTTTCTTCTTGTTAGTGATTTTGGTGGTTGGTGTTTGTTGTTTTTGTGTAGTTAGTCTTGTCTGGTGAGTGTTCTGGTCCTCCGAGATTTTTCTTTTGGTTTTCTTGTTTCCTAGTCTTGGGGTATGGATTTTCTCTAGGTTCTATCTACCTTGAGACCTGCAGCTGTTGTACAGACCTTTTTTTGATGTTTTTGGTTGTCAATACATATTTTTTCCATTAATAGTCTGTTACAATGTGTAATTTGACTGAAGGTGGGTGCTAAAGATGTCTCGGTGCACGTCCGGAAGAGCAACTCTGCAGCTCTTGGTTTGTTTGAAAAGATGGGTTACACACTTCTTGAGGTGAAGGCCAAGTATTTTGATGATGAGGAGGACGCTATTGTCTTGTGGAAGAAACTCAACGAGAAGCATATAGCCACCTAA

Protein sequence

>MS.gene51964.t1

MKQLQGTMSSKKVCIRVATIGEVLDWNSNNNLVTLPNIGRSWKKLVHVAADCSGCIVAYLLSKEEVEEEEEEEEEGNGHFCIASLFVHHTYRKRGIATDLITAAQNTMEKVGAKDVSVHVRKSNSAALGLFEKMGYTLLEVKAKYFDDEEDAIVLWKKLNEKHIAT