Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54020.t1 | XP_003588559.1 | 95.7 | 325 | 14 | 0 | 1 | 325 | 1 | 325 | 6.40E-178 | 633.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54020.t1 | Q08298 | 43.8 | 388 | 144 | 7 | 6 | 322 | 5 | 389 | 8.4e-78 | 292.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54020.t1 | B7FI52 | 95.7 | 325 | 14 | 0 | 1 | 325 | 1 | 325 | 4.6e-178 | 633.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene54020 | 0.805076 | 1.59E-49 | -1.69E-46 |
| MS.gene049151 | MS.gene54020 | 0.810053 | 1.40E-50 | -1.69E-46 |
| MS.gene049153 | MS.gene54020 | 0.804766 | 1.85E-49 | -1.69E-46 |
| MS.gene049426 | MS.gene54020 | 0.804551 | 2.05E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene54020 | 0.811464 | 6.91E-51 | -1.69E-46 |
| MS.gene049910 | MS.gene54020 | 0.810349 | 1.20E-50 | -1.69E-46 |
| MS.gene049915 | MS.gene54020 | 0.820841 | 5.54E-53 | -1.69E-46 |
| MS.gene050168 | MS.gene54020 | 0.800606 | 1.33E-48 | -1.69E-46 |
| MS.gene050226 | MS.gene54020 | 0.846937 | 1.59E-59 | -1.69E-46 |
| MS.gene050227 | MS.gene54020 | 0.826625 | 2.45E-54 | -1.69E-46 |
| MS.gene050228 | MS.gene54020 | 0.828238 | 1.00E-54 | -1.69E-46 |
| MS.gene050398 | MS.gene54020 | 0.822076 | 2.87E-53 | -1.69E-46 |
| MS.gene050442 | MS.gene54020 | 0.805615 | 1.23E-49 | -1.69E-46 |
| MS.gene050584 | MS.gene54020 | 0.80739 | 5.18E-50 | -1.69E-46 |
| MS.gene050586 | MS.gene54020 | 0.835305 | 1.81E-56 | -1.69E-46 |
| MS.gene050762 | MS.gene54020 | 0.808315 | 3.29E-50 | -1.69E-46 |
| MS.gene050763 | MS.gene54020 | 0.809523 | 1.81E-50 | -1.69E-46 |
| MS.gene051626 | MS.gene54020 | 0.821672 | 3.56E-53 | -1.69E-46 |
| MS.gene051663 | MS.gene54020 | 0.809829 | 1.56E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene54020 | 0.834513 | 2.87E-56 | -1.69E-46 |
| MS.gene051860 | MS.gene54020 | 0.814272 | 1.68E-51 | -1.69E-46 |
| MS.gene052255 | MS.gene54020 | 0.834416 | 3.04E-56 | -1.69E-46 |
| MS.gene053074 | MS.gene54020 | 0.871788 | 5.23E-67 | -1.69E-46 |
| MS.gene053184 | MS.gene54020 | 0.82733 | 1.66E-54 | -1.69E-46 |
| MS.gene053494 | MS.gene54020 | 0.809111 | 2.22E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene54020 | 0.831327 | 1.78E-55 | -1.69E-46 |
| MS.gene054184 | MS.gene54020 | 0.862781 | 3.97E-64 | -1.69E-46 |
| MS.gene054185 | MS.gene54020 | 0.867572 | 1.24E-65 | -1.69E-46 |
| MS.gene054379 | MS.gene54020 | 0.8172 | 3.73E-52 | -1.69E-46 |
| MS.gene054596 | MS.gene54020 | 0.822181 | 2.72E-53 | -1.69E-46 |
| MS.gene054930 | MS.gene54020 | 0.814524 | 1.47E-51 | -1.69E-46 |
| MS.gene055085 | MS.gene54020 | 0.807524 | 4.85E-50 | -1.69E-46 |
| MS.gene055133 | MS.gene54020 | 0.805996 | 1.02E-49 | -1.69E-46 |
| MS.gene056875 | MS.gene54020 | 0.810688 | 1.02E-50 | -1.69E-46 |
| MS.gene057394 | MS.gene54020 | 0.817069 | 3.99E-52 | -1.69E-46 |
| MS.gene057469 | MS.gene54020 | 0.813015 | 3.17E-51 | -1.69E-46 |
| MS.gene057750 | MS.gene54020 | 0.872526 | 2.97E-67 | -1.69E-46 |
| MS.gene057754 | MS.gene54020 | 0.879044 | 1.72E-69 | -1.69E-46 |
| MS.gene057869 | MS.gene54020 | 0.809755 | 1.62E-50 | -1.69E-46 |
| MS.gene057929 | MS.gene54020 | 0.800416 | 1.46E-48 | -1.69E-46 |
| MS.gene058002 | MS.gene54020 | 0.813999 | 1.93E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene54020 | 0.867551 | 1.26E-65 | -1.69E-46 |
| MS.gene059006 | MS.gene54020 | 0.853829 | 1.84E-61 | -1.69E-46 |
| MS.gene059124 | MS.gene54020 | 0.957256 | 4.21E-115 | -1.69E-46 |
| MS.gene059128 | MS.gene54020 | 0.831737 | 1.41E-55 | -1.69E-46 |
| MS.gene059421 | MS.gene54020 | 0.835517 | 1.60E-56 | -1.69E-46 |
| MS.gene060476 | MS.gene54020 | 0.810075 | 1.38E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene54020 | 0.826006 | 3.44E-54 | -1.69E-46 |
| MS.gene061049 | MS.gene54020 | 0.80564 | 1.21E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54020.t1 | MTR_0312s0030 | 95.385 | 325 | 15 | 0 | 1 | 325 | 1 | 325 | 0.0 | 647 |
| MS.gene54020.t1 | MTR_1g007520 | 95.385 | 325 | 15 | 0 | 1 | 325 | 1 | 325 | 0.0 | 647 |
| MS.gene54020.t1 | MTR_1g008590 | 95.692 | 325 | 14 | 0 | 1 | 325 | 1 | 325 | 0.0 | 620 |
| MS.gene54020.t1 | MTR_1g008510 | 75.380 | 329 | 74 | 5 | 1 | 325 | 1 | 326 | 4.02e-179 | 498 |
| MS.gene54020.t1 | MTR_1g008560 | 73.353 | 334 | 76 | 6 | 1 | 325 | 1 | 330 | 8.12e-175 | 488 |
| MS.gene54020.t1 | MTR_1g008500 | 63.496 | 389 | 74 | 7 | 1 | 325 | 1 | 385 | 1.57e-164 | 464 |
| MS.gene54020.t1 | MTR_1g008470 | 62.883 | 326 | 94 | 5 | 1 | 325 | 1 | 300 | 4.13e-142 | 403 |
| MS.gene54020.t1 | MTR_3g109490 | 59.939 | 327 | 120 | 6 | 1 | 323 | 1 | 320 | 3.55e-141 | 402 |
| MS.gene54020.t1 | MTR_1g008450 | 62.577 | 326 | 108 | 5 | 1 | 325 | 1 | 313 | 1.00e-138 | 395 |
| MS.gene54020.t1 | MTR_1g008460 | 58.788 | 330 | 98 | 7 | 1 | 325 | 1 | 297 | 1.03e-125 | 362 |
| MS.gene54020.t1 | MTR_1g008530 | 76.712 | 219 | 48 | 2 | 110 | 325 | 6 | 224 | 1.63e-122 | 351 |
| MS.gene54020.t1 | MTR_1g008580 | 79.412 | 170 | 34 | 1 | 155 | 323 | 1 | 170 | 1.09e-97 | 286 |
| MS.gene54020.t1 | MTR_3g107830 | 42.411 | 224 | 122 | 4 | 108 | 325 | 34 | 256 | 1.21e-55 | 182 |
| MS.gene54020.t1 | MTR_4g069680 | 42.857 | 224 | 121 | 5 | 108 | 325 | 65 | 287 | 3.67e-54 | 179 |
| MS.gene54020.t1 | MTR_3g116410 | 40.179 | 224 | 127 | 4 | 108 | 325 | 54 | 276 | 7.12e-53 | 175 |
| MS.gene54020.t1 | MTR_3g116380 | 42.342 | 222 | 120 | 6 | 108 | 323 | 82 | 301 | 9.23e-53 | 176 |
| MS.gene54020.t1 | MTR_3g116270 | 38.605 | 215 | 124 | 4 | 113 | 322 | 292 | 503 | 6.18e-44 | 157 |
| MS.gene54020.t1 | MTR_1g008420 | 47.475 | 198 | 76 | 6 | 8 | 178 | 3 | 199 | 1.38e-43 | 149 |
| MS.gene54020.t1 | MTR_3g116320 | 39.070 | 215 | 123 | 4 | 113 | 322 | 474 | 685 | 1.81e-43 | 159 |
| MS.gene54020.t1 | MTR_4g069570 | 37.156 | 218 | 127 | 6 | 113 | 323 | 120 | 334 | 1.04e-41 | 148 |
| MS.gene54020.t1 | MTR_4g069040 | 36.441 | 236 | 139 | 7 | 95 | 323 | 277 | 508 | 1.12e-41 | 151 |
| MS.gene54020.t1 | MTR_4g069550 | 36.697 | 218 | 128 | 6 | 113 | 323 | 247 | 461 | 4.99e-40 | 146 |
| MS.gene54020.t1 | MTR_8g064500 | 36.574 | 216 | 127 | 5 | 113 | 322 | 159 | 370 | 4.88e-38 | 139 |
| MS.gene54020.t1 | MTR_2g081610 | 29.721 | 323 | 155 | 12 | 4 | 323 | 5 | 258 | 2.23e-37 | 135 |
| MS.gene54020.t1 | MTR_2g081590 | 29.595 | 321 | 158 | 11 | 4 | 323 | 5 | 258 | 1.18e-35 | 130 |
| MS.gene54020.t1 | MTR_4g069520 | 35.321 | 218 | 131 | 6 | 113 | 323 | 507 | 721 | 1.33e-35 | 136 |
| MS.gene54020.t1 | MTR_4g069520 | 35.321 | 218 | 131 | 6 | 113 | 323 | 493 | 707 | 2.45e-35 | 135 |
| MS.gene54020.t1 | MTR_5g034320 | 33.333 | 213 | 138 | 4 | 113 | 322 | 415 | 626 | 1.18e-34 | 133 |
| MS.gene54020.t1 | MTR_8g064560 | 34.579 | 214 | 134 | 4 | 113 | 322 | 412 | 623 | 3.81e-34 | 132 |
| MS.gene54020.t1 | MTR_8g046000 | 38.073 | 218 | 103 | 9 | 112 | 323 | 30 | 221 | 8.78e-34 | 124 |
| MS.gene54020.t1 | MTR_8g445170 | 40.260 | 154 | 86 | 4 | 170 | 323 | 235 | 382 | 4.39e-33 | 126 |
| MS.gene54020.t1 | MTR_2g081770 | 28.571 | 301 | 148 | 10 | 26 | 323 | 33 | 269 | 1.37e-32 | 122 |
| MS.gene54020.t1 | MTR_8g045890 | 44.755 | 143 | 73 | 4 | 181 | 323 | 294 | 430 | 1.39e-32 | 125 |
| MS.gene54020.t1 | MTR_8g045890 | 44.056 | 143 | 74 | 4 | 181 | 323 | 335 | 471 | 2.36e-32 | 125 |
| MS.gene54020.t1 | MTR_8g045890 | 44.755 | 143 | 73 | 4 | 181 | 323 | 372 | 508 | 2.67e-32 | 126 |
| MS.gene54020.t1 | MTR_8g445140 | 41.958 | 143 | 77 | 4 | 181 | 323 | 165 | 301 | 3.12e-32 | 122 |
| MS.gene54020.t1 | MTR_3g078090 | 33.953 | 215 | 134 | 4 | 113 | 322 | 413 | 624 | 3.59e-32 | 126 |
| MS.gene54020.t1 | MTR_6g086530 | 33.493 | 209 | 128 | 7 | 125 | 325 | 1 | 206 | 1.11e-31 | 118 |
| MS.gene54020.t1 | MTR_8g064530 | 33.333 | 213 | 138 | 4 | 113 | 322 | 429 | 640 | 1.56e-31 | 124 |
| MS.gene54020.t1 | MTR_1g045860 | 27.607 | 326 | 165 | 10 | 1 | 323 | 1 | 258 | 5.90e-31 | 117 |
| MS.gene54020.t1 | MTR_4g133840 | 32.447 | 188 | 109 | 4 | 138 | 322 | 14 | 186 | 2.62e-30 | 114 |
| MS.gene54020.t1 | MTR_8g045880 | 42.759 | 145 | 77 | 4 | 179 | 323 | 175 | 313 | 1.06e-29 | 115 |
| MS.gene54020.t1 | MTR_8g045880 | 42.759 | 145 | 77 | 4 | 179 | 323 | 210 | 348 | 1.78e-29 | 115 |
| MS.gene54020.t1 | MTR_8g045880 | 42.759 | 145 | 77 | 4 | 179 | 323 | 212 | 350 | 2.21e-29 | 115 |
| MS.gene54020.t1 | MTR_8g044290 | 29.721 | 323 | 171 | 15 | 14 | 289 | 19 | 332 | 6.83e-25 | 104 |
| MS.gene54020.t1 | MTR_5g033340 | 26.852 | 216 | 152 | 4 | 111 | 322 | 164 | 377 | 9.18e-22 | 95.1 |
| MS.gene54020.t1 | MTR_5g033340 | 28.378 | 148 | 101 | 3 | 162 | 306 | 8 | 153 | 1.16e-12 | 68.6 |
| MS.gene54020.t1 | MTR_4g068420 | 35.556 | 135 | 68 | 5 | 192 | 323 | 1 | 119 | 2.18e-21 | 88.6 |
| MS.gene54020.t1 | MTR_5g033350 | 27.128 | 188 | 131 | 4 | 139 | 322 | 13 | 198 | 3.64e-19 | 84.3 |
| MS.gene54020.t1 | MTR_5g033330 | 25.926 | 216 | 154 | 4 | 111 | 322 | 403 | 616 | 7.99e-19 | 87.4 |
| MS.gene54020.t1 | MTR_5g033330 | 26.500 | 200 | 141 | 4 | 111 | 306 | 195 | 392 | 1.79e-16 | 80.5 |
| MS.gene54020.t1 | MTR_5g033330 | 26.344 | 186 | 129 | 5 | 127 | 306 | 1 | 184 | 6.14e-13 | 69.7 |
| MS.gene54020.t1 | MTR_8g075930 | 43.038 | 79 | 41 | 2 | 238 | 313 | 10 | 87 | 3.51e-17 | 75.9 |
| MS.gene54020.t1 | MTR_5g033360 | 25.116 | 215 | 139 | 5 | 111 | 322 | 25 | 220 | 7.92e-16 | 75.9 |
| MS.gene54020.t1 | MTR_5g033430 | 32.941 | 85 | 55 | 2 | 240 | 322 | 24 | 108 | 1.01e-11 | 61.6 |
| MS.gene54020.t1 | MTR_5g033440 | 32.558 | 86 | 56 | 2 | 240 | 323 | 24 | 109 | 2.50e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54020.t1 | AT5G25610 | 44.103 | 390 | 141 | 11 | 5 | 323 | 7 | 390 | 1.42e-99 | 299 |
| MS.gene54020.t1 | AT1G49320 | 37.662 | 231 | 132 | 6 | 106 | 325 | 51 | 280 | 7.07e-47 | 160 |
| MS.gene54020.t1 | AT1G60390 | 36.111 | 216 | 128 | 5 | 113 | 322 | 409 | 620 | 1.29e-34 | 133 |
| MS.gene54020.t1 | AT1G70370 | 35.514 | 214 | 132 | 4 | 113 | 322 | 411 | 622 | 2.30e-33 | 130 |
| MS.gene54020.t1 | AT1G70370 | 35.514 | 214 | 132 | 4 | 113 | 322 | 411 | 622 | 2.30e-33 | 130 |
| MS.gene54020.t1 | AT1G23760 | 34.112 | 214 | 135 | 4 | 113 | 322 | 407 | 618 | 9.83e-31 | 122 |
Find 66 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGAAATGCCAAATGTTT+TGG | 0.214978 | 1.1:+10930264 | None:intergenic |
| AAATGTAGCACTCTTCTTTC+TGG | 0.227762 | 1.1:-10930820 | MS.gene54020:CDS |
| TCTTACCTCCTCAACTTTAC+TGG | 0.262120 | 1.1:-10931651 | MS.gene54020:CDS |
| ATTTATCTGAAGCTGGATAT+TGG | 0.302370 | 1.1:-10931007 | MS.gene54020:intron |
| GCAGCATAATTATAAATAAA+TGG | 0.303775 | 1.1:+10930870 | None:intergenic |
| TGTAACATCATTGGAGTCTA+TGG | 0.322922 | 1.1:-10930565 | MS.gene54020:CDS |
| TGCCTGAGGATCATGTTGTT+TGG | 0.331746 | 1.1:-10930200 | MS.gene54020:CDS |
| AGCAGATTAGTGATGGCTTT+TGG | 0.347892 | 1.1:+10931601 | None:intergenic |
| TTCCACCTTGTTTGATGAAA+AGG | 0.348656 | 1.1:+10930698 | None:intergenic |
| TAAAGCTTACTCTGTGCCTT+TGG | 0.361974 | 1.1:-10930352 | MS.gene54020:CDS |
| AAAGCTTTGTGTAACATCAT+TGG | 0.398015 | 1.1:-10930574 | MS.gene54020:CDS |
| TGACAGTAAAACACTGCATA+AGG | 0.401155 | 1.1:+10930390 | None:intergenic |
| AGCTTACTCTGTGCCTTTGG+AGG | 0.418522 | 1.1:-10930349 | MS.gene54020:CDS |
| TCAGGCAGGAGGTGACAAAC+TGG | 0.434507 | 1.1:+10930216 | None:intergenic |
| AGAAAGAAGAGTGCTACATT+TGG | 0.440436 | 1.1:+10930822 | None:intergenic |
| CAGCAATATACAATAGCATC+TGG | 0.447095 | 1.1:-10930468 | MS.gene54020:CDS |
| GTGCCTTTGGAGGGTGCTGA+TGG | 0.447611 | 1.1:-10930339 | MS.gene54020:CDS |
| TCCACCTTGTTTGATGAAAA+GGG | 0.448369 | 1.1:+10930699 | None:intergenic |
| AGAATGGAACCCAAAACATT+TGG | 0.452431 | 1.1:-10930274 | MS.gene54020:CDS |
| AGCACTCTTCTTTCTGGAAA+AGG | 0.453598 | 1.1:-10930814 | MS.gene54020:CDS |
| TCAAATGTAGCTTGTGGTAG+TGG | 0.457950 | 1.1:-10931689 | MS.gene54020:intron |
| CTGGAAAAGGACTTGCATCA+TGG | 0.459024 | 1.1:-10930801 | MS.gene54020:CDS |
| GATTTCACTACTTCAAAGCT+TGG | 0.470526 | 1.1:-10930540 | MS.gene54020:CDS |
| GCTTACTCTGTGCCTTTGGA+GGG | 0.480708 | 1.1:-10930348 | MS.gene54020:CDS |
| TGTGAAGAGCAAGGGATCAA+AGG | 0.490839 | 1.1:-10930603 | MS.gene54020:CDS |
| CAAGTACTCAAAGTTCAACC+TGG | 0.499411 | 1.1:-10930246 | MS.gene54020:CDS |
| GCATCTGGAGTGAAGAAGTT+GGG | 0.513820 | 1.1:-10930453 | MS.gene54020:CDS |
| ATCCAAACAACATGATCCTC+AGG | 0.517927 | 1.1:+10930198 | None:intergenic |
| GGAGTGAAGAGAAAGGAACT+TGG | 0.518251 | 1.1:-10930986 | MS.gene54020:CDS |
| GTCAGAAAGGGATACTATGA+AGG | 0.523139 | 1.1:-10930930 | MS.gene54020:CDS |
| GACAGTAAAACACTGCATAA+GGG | 0.527238 | 1.1:+10930391 | None:intergenic |
| GGCAAGGGTGGTGTAGATGT+TGG | 0.529745 | 1.1:-10930954 | MS.gene54020:CDS |
| AAAATCAAGAAAGGGTCAAA+GGG | 0.531552 | 1.1:-10930657 | MS.gene54020:CDS |
| AGCATCTGGAGTGAAGAAGT+TGG | 0.536857 | 1.1:-10930454 | MS.gene54020:CDS |
| TGTAGATGTTGGAGTCAGAA+AGG | 0.539383 | 1.1:-10930943 | MS.gene54020:CDS |
| CATTGAAGAGGTGGGAAGCA+TGG | 0.540494 | 1.1:+10931624 | None:intergenic |
| TGACAAACTGGAACAGTCCC+AGG | 0.541759 | 1.1:+10930228 | None:intergenic |
| AAAGGAACTTGGGTGGATGT+AGG | 0.554611 | 1.1:-10930975 | MS.gene54020:CDS |
| GGATATTGGAGTGAAGAGAA+AGG | 0.556237 | 1.1:-10930993 | MS.gene54020:CDS |
| AACTTGGGTGGATGTAGGCA+AGG | 0.557293 | 1.1:-10930970 | MS.gene54020:CDS |
| AGTTTGTCACCTCCTGCCTG+AGG | 0.562196 | 1.1:-10930214 | MS.gene54020:CDS |
| GTAGATGTTGGAGTCAGAAA+GGG | 0.562316 | 1.1:-10930942 | MS.gene54020:CDS |
| GTCAGTGAGTGTGAAGAGCA+AGG | 0.568006 | 1.1:-10930612 | MS.gene54020:CDS |
| CTTCCATCAGCACCCTCCAA+AGG | 0.576677 | 1.1:+10930336 | None:intergenic |
| TGAAGAGAAAGGAACTTGGG+TGG | 0.577433 | 1.1:-10930982 | MS.gene54020:CDS |
| TTGCTCTTCACACTCACTGA+CGG | 0.578201 | 1.1:+10930614 | None:intergenic |
| TGGGTGGATGTAGGCAAGGG+TGG | 0.579389 | 1.1:-10930966 | MS.gene54020:CDS |
| GAGTGAAGAGAAAGGAACTT+GGG | 0.579403 | 1.1:-10930985 | MS.gene54020:CDS |
| GGTGGCTCTGATGTGAATGT+TGG | 0.588281 | 1.1:-10930906 | MS.gene54020:CDS |
| TCAGTGAGTGTGAAGAGCAA+GGG | 0.599484 | 1.1:-10930611 | MS.gene54020:CDS |
| AAGGGATACTATGAAGGAGG+TGG | 0.599632 | 1.1:-10930924 | MS.gene54020:CDS |
| AACTTGGCGCGGCAAGAATG+TGG | 0.603165 | 1.1:+10930734 | None:intergenic |
| GACTTCCAGTAAAGTTGAGG+AGG | 0.605659 | 1.1:+10931646 | None:intergenic |
| GTCACACTGATACATCAGAA+TGG | 0.606500 | 1.1:-10930290 | MS.gene54020:CDS |
| AAACAACATGATCCTCAGGC+AGG | 0.608017 | 1.1:+10930202 | None:intergenic |
| TCTGATGTGAATGTTGGAGT+TGG | 0.609740 | 1.1:-10930900 | MS.gene54020:CDS |
| ATGGACTTCCAGTAAAGTTG+AGG | 0.616898 | 1.1:+10931643 | None:intergenic |
| AGGGTATTGAATTAGCAACT+TGG | 0.619732 | 1.1:+10930718 | None:intergenic |
| AAGTACTCAAAGTTCAACCT+GGG | 0.622856 | 1.1:-10930245 | MS.gene54020:CDS |
| AGGGTGTAGCAGATTAGTGA+TGG | 0.623388 | 1.1:+10931594 | None:intergenic |
| ATTGAATTAGCAACTTGGCG+CGG | 0.637675 | 1.1:+10930723 | None:intergenic |
| CAACATGATCCTCAGGCAGG+AGG | 0.654087 | 1.1:+10930205 | None:intergenic |
| ACTTGGGTGGATGTAGGCAA+GGG | 0.659855 | 1.1:-10930969 | MS.gene54020:CDS |
| ATCACTAATCTGCTACACCC+TGG | 0.665412 | 1.1:-10931592 | MS.gene54020:intron |
| AGAGCAAGGGATCAAAGGAG+AGG | 0.677572 | 1.1:-10930598 | MS.gene54020:CDS |
| AGAAAGGGATACTATGAAGG+AGG | 0.679610 | 1.1:-10930927 | MS.gene54020:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAATAAACCAAGTAAT+TGG | - | chr1.1:10930789-10930808 | MS.gene54020:CDS | 15.0% |
| !! | AATAAATAAACCAAGTAATT+GGG | - | chr1.1:10930790-10930809 | MS.gene54020:CDS | 15.0% |
| !! | GTTAATTATTTGAAGAAAAT+TGG | + | chr1.1:10930255-10930274 | None:intergenic | 15.0% |
| !! | TTAAATAAATAAAAAGTCTG+TGG | - | chr1.1:10930923-10930942 | MS.gene54020:CDS | 15.0% |
| !!! | ATACTCATTTTATATATAGA+TGG | - | chr1.1:10930487-10930506 | MS.gene54020:CDS | 15.0% |
| !!! | GTTTAAAATGATTTCATTTT+AGG | - | chr1.1:10930526-10930545 | MS.gene54020:CDS | 15.0% |
| !! | AACAAGTTAAAAATCAAGAA+AGG | - | chr1.1:10931341-10931360 | MS.gene54020:intron | 20.0% |
| !! | ACAAGTTAAAAATCAAGAAA+GGG | - | chr1.1:10931342-10931361 | MS.gene54020:intron | 20.0% |
| !! | GCAGCATAATTATAAATAAA+TGG | + | chr1.1:10931140-10931159 | None:intergenic | 20.0% |
| !!! | ATGAAATCATTTTAAACACA+TGG | + | chr1.1:10930523-10930542 | None:intergenic | 20.0% |
| ! | AAAGGACTTATGATATATGA+AGG | - | chr1.1:10930756-10930775 | MS.gene54020:CDS | 25.0% |
| ! | ACCATGAAAAAAGTAATGAT+GGG | + | chr1.1:10930209-10930228 | None:intergenic | 25.0% |
| ! | ATTTAATTCATGCACATTGT+CGG | - | chr1.1:10930549-10930568 | MS.gene54020:CDS | 25.0% |
| !!! | TATTTTTTGTGTTAACTCTC+CGG | + | chr1.1:10930903-10930922 | None:intergenic | 25.0% |
| AAAAATCAAGAAAGGGTCAA+AGG | - | chr1.1:10931349-10931368 | MS.gene54020:intron | 30.0% | |
| AAAATCAAGAAAGGGTCAAA+GGG | - | chr1.1:10931350-10931369 | MS.gene54020:intron | 30.0% | |
| AAAGCTTGGAAAAAATGTTG+AGG | - | chr1.1:10931481-10931500 | MS.gene54020:intron | 30.0% | |
| CACCATGAAAAAAGTAATGA+TGG | + | chr1.1:10930210-10930229 | None:intergenic | 30.0% | |
| TTGAATTATTCGTCATAGAG+AGG | + | chr1.1:10930833-10930852 | None:intergenic | 30.0% | |
| ! | AAAGCTTTGTGTAACATCAT+TGG | - | chr1.1:10931433-10931452 | MS.gene54020:intron | 30.0% |
| ! | AATACCCTTTTCATCAAACA+AGG | - | chr1.1:10931304-10931323 | MS.gene54020:intron | 30.0% |
| ! | ACTTGAAATGCCAAATGTTT+TGG | + | chr1.1:10931746-10931765 | None:intergenic | 30.0% |
| ! | ATTTATCTGAAGCTGGATAT+TGG | - | chr1.1:10931000-10931019 | MS.gene54020:intron | 30.0% |
| ! | CTTGAAATGCCAAATGTTTT+GGG | + | chr1.1:10931745-10931764 | None:intergenic | 30.0% |
| ! | TTACTCTATTTATCTGAAGC+TGG | - | chr1.1:10930993-10931012 | MS.gene54020:CDS | 30.0% |
| !!! | ATTTTTTGTGTTAACTCTCC+GGG | + | chr1.1:10930902-10930921 | None:intergenic | 30.0% |
| !!! | TCCCATCATTACTTTTTTCA+TGG | - | chr1.1:10930205-10930224 | MS.gene54020:CDS | 30.0% |
| AAATGTAGCACTCTTCTTTC+TGG | - | chr1.1:10931187-10931206 | MS.gene54020:intron | 35.0% | |
| AAGTACTCAAAGTTCAACCT+GGG | - | chr1.1:10931762-10931781 | MS.gene54020:intron | 35.0% | |
| ACCAAGTAATTGGGTTTAAG+TGG | - | chr1.1:10930799-10930818 | MS.gene54020:CDS | 35.0% | |
| ACCACTTAAACCCAATTACT+TGG | + | chr1.1:10930803-10930822 | None:intergenic | 35.0% | |
| AGAATGGAACCCAAAACATT+TGG | - | chr1.1:10931733-10931752 | MS.gene54020:intron | 35.0% | |
| CAGCAATATACAATAGCATC+TGG | - | chr1.1:10931539-10931558 | MS.gene54020:intron | 35.0% | |
| CTAGACTATGATACTAACCA+GGG | + | chr1.1:10930435-10930454 | None:intergenic | 35.0% | |
| CTATTCTCAAATGTAGCTTG+TGG | - | chr1.1:10930312-10930331 | MS.gene54020:CDS | 35.0% | |
| GACAGTAAAACACTGCATAA+GGG | + | chr1.1:10931619-10931638 | None:intergenic | 35.0% | |
| GATTTCACTACTTCAAAGCT+TGG | - | chr1.1:10931467-10931486 | MS.gene54020:intron | 35.0% | |
| TCAGATGATCTCTATCAGAA+AGG | - | chr1.1:10930738-10930757 | MS.gene54020:CDS | 35.0% | |
| TCTAGACTATGATACTAACC+AGG | + | chr1.1:10930436-10930455 | None:intergenic | 35.0% | |
| TGACAGTAAAACACTGCATA+AGG | + | chr1.1:10931620-10931639 | None:intergenic | 35.0% | |
| TGTAACATCATTGGAGTCTA+TGG | - | chr1.1:10931442-10931461 | MS.gene54020:intron | 35.0% | |
| ! | TCCACCTTGTTTGATGAAAA+GGG | + | chr1.1:10931311-10931330 | None:intergenic | 35.0% |
| ! | TTCCACCTTGTTTGATGAAA+AGG | + | chr1.1:10931312-10931331 | None:intergenic | 35.0% |
| !! | AGAAAGAAGAGTGCTACATT+TGG | + | chr1.1:10931188-10931207 | None:intergenic | 35.0% |
| !! | AGGGTATTGAATTAGCAACT+TGG | + | chr1.1:10931292-10931311 | None:intergenic | 35.0% |
| !!! | TTTTTTGTGTTAACTCTCCG+GGG | + | chr1.1:10930901-10930920 | None:intergenic | 35.0% |
| AGAAAGGGATACTATGAAGG+AGG | - | chr1.1:10931080-10931099 | MS.gene54020:intron | 40.0% | |
| ATCCAAACAACATGATCCTC+AGG | + | chr1.1:10931812-10931831 | None:intergenic | 40.0% | |
| ATGGACTTCCAGTAAAGTTG+AGG | + | chr1.1:10930367-10930386 | None:intergenic | 40.0% | |
| ATTGAATTAGCAACTTGGCG+CGG | + | chr1.1:10931287-10931306 | None:intergenic | 40.0% | |
| CAAGTACTCAAAGTTCAACC+TGG | - | chr1.1:10931761-10931780 | MS.gene54020:intron | 40.0% | |
| GAGTGAAGAGAAAGGAACTT+GGG | - | chr1.1:10931022-10931041 | MS.gene54020:intron | 40.0% | |
| GGATATTGGAGTGAAGAGAA+AGG | - | chr1.1:10931014-10931033 | MS.gene54020:intron | 40.0% | |
| GTAGATGTTGGAGTCAGAAA+GGG | - | chr1.1:10931065-10931084 | MS.gene54020:intron | 40.0% | |
| GTCACACTGATACATCAGAA+TGG | - | chr1.1:10931717-10931736 | MS.gene54020:intron | 40.0% | |
| GTCAGAAAGGGATACTATGA+AGG | - | chr1.1:10931077-10931096 | MS.gene54020:intron | 40.0% | |
| TAAAGCTTACTCTGTGCCTT+TGG | - | chr1.1:10931655-10931674 | MS.gene54020:CDS | 40.0% | |
| TCTGATGTGAATGTTGGAGT+TGG | - | chr1.1:10931107-10931126 | MS.gene54020:intron | 40.0% | |
| TCTTACCTCCTCAACTTTAC+TGG | - | chr1.1:10930356-10930375 | MS.gene54020:CDS | 40.0% | |
| TGTAGATGTTGGAGTCAGAA+AGG | - | chr1.1:10931064-10931083 | MS.gene54020:intron | 40.0% | |
| ! | ACCCTTTTCATCAAACAAGG+TGG | - | chr1.1:10931307-10931326 | MS.gene54020:intron | 40.0% |
| ! | AGCACTCTTCTTTCTGGAAA+AGG | - | chr1.1:10931193-10931212 | MS.gene54020:intron | 40.0% |
| ! | AGCAGATTAGTGATGGCTTT+TGG | + | chr1.1:10930409-10930428 | None:intergenic | 40.0% |
| ! | TCAAATGTAGCTTGTGGTAG+TGG | - | chr1.1:10930318-10930337 | MS.gene54020:CDS | 40.0% |
| AAACAACATGATCCTCAGGC+AGG | + | chr1.1:10931808-10931827 | None:intergenic | 45.0% | |
| AAAGGAACTTGGGTGGATGT+AGG | - | chr1.1:10931032-10931051 | MS.gene54020:intron | 45.0% | |
| AAGGGATACTATGAAGGAGG+TGG | - | chr1.1:10931083-10931102 | MS.gene54020:intron | 45.0% | |
| AGGGTGTAGCAGATTAGTGA+TGG | + | chr1.1:10930416-10930435 | None:intergenic | 45.0% | |
| ATCACTAATCTGCTACACCC+TGG | - | chr1.1:10930415-10930434 | MS.gene54020:CDS | 45.0% | |
| CTGGAAAAGGACTTGCATCA+TGG | - | chr1.1:10931206-10931225 | MS.gene54020:intron | 45.0% | |
| GACTTCCAGTAAAGTTGAGG+AGG | + | chr1.1:10930364-10930383 | None:intergenic | 45.0% | |
| GGAGTGAAGAGAAAGGAACT+TGG | - | chr1.1:10931021-10931040 | MS.gene54020:intron | 45.0% | |
| TCAGTGAGTGTGAAGAGCAA+GGG | - | chr1.1:10931396-10931415 | MS.gene54020:intron | 45.0% | |
| TGAAGAGAAAGGAACTTGGG+TGG | - | chr1.1:10931025-10931044 | MS.gene54020:intron | 45.0% | |
| TGCCTGAGGATCATGTTGTT+TGG | - | chr1.1:10931807-10931826 | MS.gene54020:CDS | 45.0% | |
| TGTGAAGAGCAAGGGATCAA+AGG | - | chr1.1:10931404-10931423 | MS.gene54020:intron | 45.0% | |
| TTGCTCTTCACACTCACTGA+CGG | + | chr1.1:10931396-10931415 | None:intergenic | 45.0% | |
| ! | AGCATCTGGAGTGAAGAAGT+TGG | - | chr1.1:10931553-10931572 | MS.gene54020:intron | 45.0% |
| ! | GCATCTGGAGTGAAGAAGTT+GGG | - | chr1.1:10931554-10931573 | MS.gene54020:intron | 45.0% |
| !! | GATGGCTTTTGGCATTGAAG+AGG | + | chr1.1:10930398-10930417 | None:intergenic | 45.0% |
| !! | GCTTTTGGCATTGAAGAGGT+GGG | + | chr1.1:10930394-10930413 | None:intergenic | 45.0% |
| AACTTGGGTGGATGTAGGCA+AGG | - | chr1.1:10931037-10931056 | MS.gene54020:intron | 50.0% | |
| ACTTGGGTGGATGTAGGCAA+GGG | - | chr1.1:10931038-10931057 | MS.gene54020:intron | 50.0% | |
| AGAGCAAGGGATCAAAGGAG+AGG | - | chr1.1:10931409-10931428 | MS.gene54020:intron | 50.0% | |
| AGCTTACTCTGTGCCTTTGG+AGG | - | chr1.1:10931658-10931677 | MS.gene54020:CDS | 50.0% | |
| CATTGAAGAGGTGGGAAGCA+TGG | + | chr1.1:10930386-10930405 | None:intergenic | 50.0% | |
| GCTTACTCTGTGCCTTTGGA+GGG | - | chr1.1:10931659-10931678 | MS.gene54020:CDS | 50.0% | |
| GGAAGGGGGTCATAGTAATC+AGG | + | chr1.1:10930871-10930890 | None:intergenic | 50.0% | |
| GTCAGTGAGTGTGAAGAGCA+AGG | - | chr1.1:10931395-10931414 | MS.gene54020:intron | 50.0% | |
| GTTAACTCTCCGGGGTCTAA+GGG | + | chr1.1:10930893-10930912 | None:intergenic | 50.0% | |
| TGACAAACTGGAACAGTCCC+AGG | + | chr1.1:10931782-10931801 | None:intergenic | 50.0% | |
| TGTTAACTCTCCGGGGTCTA+AGG | + | chr1.1:10930894-10930913 | None:intergenic | 50.0% | |
| TTAACTCTCCGGGGTCTAAG+GGG | + | chr1.1:10930892-10930911 | None:intergenic | 50.0% | |
| !! | GGCTTTTGGCATTGAAGAGG+TGG | + | chr1.1:10930395-10930414 | None:intergenic | 50.0% |
| !! | GGTGGCTCTGATGTGAATGT+TGG | - | chr1.1:10931101-10931120 | MS.gene54020:intron | 50.0% |
| AACTTGGCGCGGCAAGAATG+TGG | + | chr1.1:10931276-10931295 | None:intergenic | 55.0% | |
| AGTTTGTCACCTCCTGCCTG+AGG | - | chr1.1:10931793-10931812 | MS.gene54020:intron | 55.0% | |
| CAACATGATCCTCAGGCAGG+AGG | + | chr1.1:10931805-10931824 | None:intergenic | 55.0% | |
| CTTCCATCAGCACCCTCCAA+AGG | + | chr1.1:10931674-10931693 | None:intergenic | 55.0% | |
| GGCAAGGGTGGTGTAGATGT+TGG | - | chr1.1:10931053-10931072 | MS.gene54020:intron | 55.0% | |
| TCAGGCAGGAGGTGACAAAC+TGG | + | chr1.1:10931794-10931813 | None:intergenic | 55.0% | |
| TCTCCGGGGTCTAAGGGGAA+GGG | + | chr1.1:10930887-10930906 | None:intergenic | 60.0% | |
| TGGGTGGATGTAGGCAAGGG+TGG | - | chr1.1:10931041-10931060 | MS.gene54020:intron | 60.0% | |
| !! | GTGCCTTTGGAGGGTGCTGA+TGG | - | chr1.1:10931668-10931687 | MS.gene54020:CDS | 60.0% |
| ACCCCCTTCCCCTTAGACCC+CGG | - | chr1.1:10930881-10930900 | MS.gene54020:CDS | 65.0% | |
| CTCCGGGGTCTAAGGGGAAG+GGG | + | chr1.1:10930886-10930905 | None:intergenic | 65.0% | |
| CTCTCCGGGGTCTAAGGGGA+AGG | + | chr1.1:10930888-10930907 | None:intergenic | 65.0% | |
| TCCGGGGTCTAAGGGGAAGG+GGG | + | chr1.1:10930885-10930904 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 10930188 | 10931841 | 10930188 | ID=MS.gene54020 |
| chr1.1 | mRNA | 10930188 | 10931841 | 10930188 | ID=MS.gene54020.t1;Parent=MS.gene54020 |
| chr1.1 | exon | 10931803 | 10931841 | 10931803 | ID=MS.gene54020.t1.exon1;Parent=MS.gene54020.t1 |
| chr1.1 | CDS | 10931803 | 10931841 | 10931803 | ID=cds.MS.gene54020.t1;Parent=MS.gene54020.t1 |
| chr1.1 | exon | 10931593 | 10931701 | 10931593 | ID=MS.gene54020.t1.exon2;Parent=MS.gene54020.t1 |
| chr1.1 | CDS | 10931593 | 10931701 | 10931593 | ID=cds.MS.gene54020.t1;Parent=MS.gene54020.t1 |
| chr1.1 | exon | 10930188 | 10931017 | 10930188 | ID=MS.gene54020.t1.exon3;Parent=MS.gene54020.t1 |
| chr1.1 | CDS | 10930188 | 10931017 | 10930188 | ID=cds.MS.gene54020.t1;Parent=MS.gene54020.t1 |
| Gene Sequence |
| Protein sequence |
