AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049426


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049426.t1 MTR_3g093570 95.349 129 3 1 2 127 287 415 1.89e-82 249
MS.gene049426.t1 MTR_3g093570 94.286 70 4 0 2 71 287 356 3.39e-40 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049426.t1 AT3G10840 59.690 129 48 2 3 127 206 334 9.53e-48 158
MS.gene049426.t1 AT3G10840 59.690 129 48 2 3 127 206 334 9.53e-48 158
MS.gene049426.t1 AT3G10840 59.690 129 48 2 3 127 220 348 1.18e-47 158
MS.gene049426.t1 AT3G10840 59.690 129 48 2 3 127 281 409 3.20e-47 158

Find 34 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCACTGCTGCAATGCTTC+TGG 0.272675 3.1:-74913372 MS.gene049426:CDS
ACATGCTCAGAGACCTGTTT+TGG 0.311785 3.1:+74913566 None:intergenic
TCTATCCTGCGTTCTTCCCC+TGG 0.359604 3.1:-74913998 MS.gene049426:CDS
GCCATTGAGGGTTAAGGATT+GGG 0.400126 3.1:-74913411 MS.gene049426:intron
GTGATCGACTAGTTCCTTCA+TGG 0.407021 3.1:-74913154 MS.gene049426:CDS
AGAATGGCAATTGATAAGTT+TGG 0.426361 3.1:-74913620 MS.gene049426:CDS
GTCTCTGAGCATGTCCTTAG+TGG 0.450535 3.1:-74913557 MS.gene049426:CDS
TGCTGCAATGCTTCTGGACG+AGG 0.462142 3.1:-74913366 MS.gene049426:CDS
AATGCAGCCATTGAGGGTTA+AGG 0.463788 3.1:-74913417 MS.gene049426:intron
AGCCATTGAGGGTTAAGGAT+TGG 0.464429 3.1:-74913412 MS.gene049426:intron
ATTACCTTTGATAACCACTA+AGG 0.481852 3.1:+74913543 None:intergenic
TTCATGGTATGATCCAAAAC+AGG 0.485270 3.1:-74913579 MS.gene049426:CDS
TAAGGATTGGGATAGGGCAC+TGG 0.491068 3.1:-74913399 MS.gene049426:CDS
CTGCTGCAGTTCGAAATTCA+TGG 0.508279 3.1:-74913595 MS.gene049426:CDS
ATATGAATCTCAGGTAAGAA+TGG 0.520846 3.1:-74913636 MS.gene049426:intron
GAGGGTTAAGGATTGGGATA+GGG 0.521224 3.1:-74913405 MS.gene049426:CDS
ATTACTTCAAACGATGCTCC+TGG 0.525094 3.1:+74913103 None:intergenic
TGTGCAGTTCTGATTGTAAC+CGG 0.531619 3.1:-74913182 MS.gene049426:intron
TGAGGGTTAAGGATTGGGAT+AGG 0.534539 3.1:-74913406 MS.gene049426:intron
CTATCCTGCGTTCTTCCCCT+GGG 0.534716 3.1:-74913997 MS.gene049426:CDS
TTCTTCCCCTGGGATAATGC+TGG 0.549567 3.1:-74913987 MS.gene049426:intron
CTGAATGAAATATCTTGTCC+TGG 0.551019 3.1:-74913311 MS.gene049426:intron
GTCCTCTCAGCATTCCATGA+AGG 0.554033 3.1:+74913140 None:intergenic
GAGTTGAGTATATTTATCAT+CGG 0.568376 3.1:+74914043 None:intergenic
TGTATTACCAGCATTATCCC+AGG 0.576948 3.1:+74913980 None:intergenic
GGATTGGGATAGGGCACTGG+TGG 0.577488 3.1:-74913396 MS.gene049426:CDS
AACTAGTCGATCACTATCAC+CGG 0.578562 3.1:+74913163 None:intergenic
TTATCCCAGGGGAAGAACGC+AGG 0.590594 3.1:+74913993 None:intergenic
GATTATCATGTACATTGCAA+AGG 0.604945 3.1:-74914086 None:intergenic
GGACTTTCAAGAGTCATACC+AGG 0.610920 3.1:-74913121 MS.gene049426:CDS
ATGTCCTTAGTGGTTATCAA+AGG 0.619928 3.1:-74913547 MS.gene049426:intron
GTATTACCAGCATTATCCCA+GGG 0.631989 3.1:+74913981 None:intergenic
TTCCTTCATGGAATGCTGAG+AGG 0.647412 3.1:-74913142 MS.gene049426:CDS
TATTACCAGCATTATCCCAG+GGG 0.766035 3.1:+74913982 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAAAATATTTCATTAA+GGG + chr3.1:74913921-74913940 None:intergenic 10.0%
!! CAGAAAAAAATATTTCATTA+AGG + chr3.1:74913922-74913941 None:intergenic 15.0%
!! AGTTACAGAATATGAAAATA+GGG + chr3.1:74913695-74913714 None:intergenic 20.0%
!! ATAATAATCGAAAAGAAACA+TGG - chr3.1:74913293-74913312 MS.gene049426:intron 20.0%
!! TAGTTACAGAATATGAAAAT+AGG + chr3.1:74913696-74913715 None:intergenic 20.0%
! GAGTTGAGTATATTTATCAT+CGG + chr3.1:74913154-74913173 None:intergenic 25.0%
! GCCATGACAAAATTATATAT+GGG + chr3.1:74913513-74913532 None:intergenic 25.0%
! TGAGATTCATATGAAAGAAA+GGG + chr3.1:74913550-74913569 None:intergenic 25.0%
! TTTCCAATAATGATATGTAC+TGG + chr3.1:74913271-74913290 None:intergenic 25.0%
!! ACCCATATATAATTTTGTCA+TGG - chr3.1:74913509-74913528 MS.gene049426:intron 25.0%
AATATCACCAATTATGACCA+AGG + chr3.1:74913985-74914004 None:intergenic 30.0%
AGAATGGCAATTGATAAGTT+TGG - chr3.1:74913574-74913593 MS.gene049426:CDS 30.0%
AGCTATGTGAGGAAAAAATA+TGG - chr3.1:74913413-74913432 MS.gene049426:intron 30.0%
ATATGAATCTCAGGTAAGAA+TGG - chr3.1:74913558-74913577 MS.gene049426:CDS 30.0%
CTGAGATTCATATGAAAGAA+AGG + chr3.1:74913551-74913570 None:intergenic 30.0%
CTTTCTTTCATATGAATCTC+AGG - chr3.1:74913549-74913568 MS.gene049426:CDS 30.0%
GGCCATGACAAAATTATATA+TGG + chr3.1:74913514-74913533 None:intergenic 30.0%
GTTCCAGTACATATCATTAT+TGG - chr3.1:74913265-74913284 MS.gene049426:intron 30.0%
! ATTACCTTTGATAACCACTA+AGG + chr3.1:74913654-74913673 None:intergenic 30.0%
! CATGAAGACAAGGAATTTTA+AGG + chr3.1:74913380-74913399 None:intergenic 30.0%
!! TTCAGTCTTTTAGAAAGTTC+TGG + chr3.1:74913868-74913887 None:intergenic 30.0%
AGTTGAACCTTGGTCATAAT+TGG - chr3.1:74913975-74913994 MS.gene049426:intron 35.0%
ATGTCCTTAGTGGTTATCAA+AGG - chr3.1:74913647-74913666 MS.gene049426:intron 35.0%
CTGAATGAAATATCTTGTCC+TGG - chr3.1:74913883-74913902 MS.gene049426:intron 35.0%
CTGTTGTGTAAAGATCTAAC+TGG - chr3.1:74913939-74913958 MS.gene049426:intron 35.0%
TATGTATGGACATGAAGACA+AGG + chr3.1:74913390-74913409 None:intergenic 35.0%
TTCATGGTATGATCCAAAAC+AGG - chr3.1:74913615-74913634 MS.gene049426:CDS 35.0%
! GGAACATTGATTGAATGACA+AGG + chr3.1:74913359-74913378 None:intergenic 35.0%
! TATAATTTTGTCATGGCCAC+TGG - chr3.1:74913516-74913535 MS.gene049426:intron 35.0%
AACTAGTCGATCACTATCAC+CGG + chr3.1:74914034-74914053 None:intergenic 40.0%
CATACATAGTCAGCTATGTG+AGG - chr3.1:74913402-74913421 MS.gene049426:CDS 40.0%
CTCAAATAGTCTGTGCTTAC+TGG - chr3.1:74913743-74913762 MS.gene049426:intron 40.0%
CTCACATAGCTGACTATGTA+TGG + chr3.1:74913404-74913423 None:intergenic 40.0%
GTATTACCAGCATTATCCCA+GGG + chr3.1:74913216-74913235 None:intergenic 40.0%
TATGACCAAGGTTCAACTTG+AGG + chr3.1:74913973-74913992 None:intergenic 40.0%
TATTACCAGCATTATCCCAG+GGG + chr3.1:74913215-74913234 None:intergenic 40.0%
TGTATTACCAGCATTATCCC+AGG + chr3.1:74913217-74913236 None:intergenic 40.0%
! TGTGCAGTTCTGATTGTAAC+CGG - chr3.1:74914012-74914031 MS.gene049426:CDS 40.0%
AATGCAGCCATTGAGGGTTA+AGG - chr3.1:74913777-74913796 MS.gene049426:intron 45.0%
AGCCATTGAGGGTTAAGGAT+TGG - chr3.1:74913782-74913801 MS.gene049426:intron 45.0%
ATTCACTGCTGCAATGCTTC+TGG - chr3.1:74913822-74913841 MS.gene049426:intron 45.0%
CTGCTGCAGTTCGAAATTCA+TGG - chr3.1:74913599-74913618 MS.gene049426:CDS 45.0%
GAGGGTTAAGGATTGGGATA+GGG - chr3.1:74913789-74913808 MS.gene049426:intron 45.0%
GCCATTGAGGGTTAAGGATT+GGG - chr3.1:74913783-74913802 MS.gene049426:intron 45.0%
GGACTTTCAAGAGTCATACC+AGG - chr3.1:74914073-74914092 MS.gene049426:CDS 45.0%
TAAGGGTCAGTGAAATCACC+AGG + chr3.1:74913904-74913923 None:intergenic 45.0%
TCCCAATCCTTAACCCTCAA+TGG + chr3.1:74913787-74913806 None:intergenic 45.0%
TGAGGGTTAAGGATTGGGAT+AGG - chr3.1:74913788-74913807 MS.gene049426:intron 45.0%
TTCCTTCATGGAATGCTGAG+AGG - chr3.1:74914052-74914071 MS.gene049426:CDS 45.0%
! ACATGCTCAGAGACCTGTTT+TGG + chr3.1:74913631-74913650 None:intergenic 45.0%
! GTGATCGACTAGTTCCTTCA+TGG - chr3.1:74914040-74914059 MS.gene049426:CDS 45.0%
! TGTGCTTACTGGTGTTAGCA+TGG - chr3.1:74913754-74913773 MS.gene049426:intron 45.0%
!! CATAGCCTCAAGTTGAACCT+TGG - chr3.1:74913965-74913984 MS.gene049426:intron 45.0%
AGAAAGGGCCATTAGTCCAG+TGG + chr3.1:74913535-74913554 None:intergenic 50.0%
AGCATGGAATGCAGCCATTG+AGG - chr3.1:74913770-74913789 MS.gene049426:intron 50.0%
GCATGGAATGCAGCCATTGA+GGG - chr3.1:74913771-74913790 MS.gene049426:intron 50.0%
GTCCTCTCAGCATTCCATGA+AGG + chr3.1:74914057-74914076 None:intergenic 50.0%
GTCTCTGAGCATGTCCTTAG+TGG - chr3.1:74913637-74913656 MS.gene049426:intron 50.0%
TAAGGATTGGGATAGGGCAC+TGG - chr3.1:74913795-74913814 MS.gene049426:intron 50.0%
TGTCATGGCCACTGGACTAA+TGG - chr3.1:74913524-74913543 MS.gene049426:intron 50.0%
TTCTTCCCCTGGGATAATGC+TGG - chr3.1:74913207-74913226 MS.gene049426:intron 50.0%
CTATCCTGCGTTCTTCCCCT+GGG - chr3.1:74913197-74913216 MS.gene049426:intron 55.0%
TCTATCCTGCGTTCTTCCCC+TGG - chr3.1:74913196-74913215 MS.gene049426:intron 55.0%
TGCTGCAATGCTTCTGGACG+AGG - chr3.1:74913828-74913847 MS.gene049426:intron 55.0%
TTATCCCAGGGGAAGAACGC+AGG + chr3.1:74913204-74913223 None:intergenic 55.0%
!! GGATTGGGATAGGGCACTGG+TGG - chr3.1:74913798-74913817 MS.gene049426:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 74913115 74914101 74913115 ID=MS.gene049426
chr3.1 mRNA 74913115 74914101 74913115 ID=MS.gene049426.t1;Parent=MS.gene049426
chr3.1 exon 74913988 74914101 74913988 ID=MS.gene049426.t1.exon1;Parent=MS.gene049426.t1
chr3.1 CDS 74913988 74914101 74913988 ID=cds.MS.gene049426.t1;Parent=MS.gene049426.t1
chr3.1 exon 74913548 74913645 74913548 ID=MS.gene049426.t1.exon2;Parent=MS.gene049426.t1
chr3.1 CDS 74913548 74913645 74913548 ID=cds.MS.gene049426.t1;Parent=MS.gene049426.t1
chr3.1 exon 74913312 74913424 74913312 ID=MS.gene049426.t1.exon3;Parent=MS.gene049426.t1
chr3.1 CDS 74913312 74913424 74913312 ID=cds.MS.gene049426.t1;Parent=MS.gene049426.t1
chr3.1 exon 74913115 74913197 74913115 ID=MS.gene049426.t1.exon4;Parent=MS.gene049426.t1
chr3.1 CDS 74913115 74913197 74913115 ID=cds.MS.gene049426.t1;Parent=MS.gene049426.t1
Gene Sequence

>MS.gene049426

ATGTACATTGCAAAGGCAATAGCAGAGATGATGAAGCCGATGATAAATATACTCAACTCTCTCTATAAGAAACTGTTATCATCTATCCTGCGTTCTTCCCCTGGGATAATGCTGGTAATACACTTGTTCATGTAATACTATATAATCTTTGTTCCAGTACATATCATTATTGGAAAATATAATAATCGAAAAGAAACATGGAATTATTAGCTCAGATACAATTACATTGAACTCACAAAAACCTTGTCATTCAATCAATGTTCCTTAAAATTCCTTGTCTTCATGTCCATACATAGTCAGCTATGTGAGGAAAAAATATGGTTAGATTGCTTCTTGCTGATACACTTTTATGCATTTTAGTTTCTATCTTTAAATGACTGCAATTCTTAAGCTGACCCATATATAATTTTGTCATGGCCACTGGACTAATGGCCCTTTCTTTCATATGAATCTCAGGTAAGAATGGCAATTGATAAGTTTGGTGCTGCTGCAGTTCGAAATTCATGGTATGATCCAAAACAGGTCTCTGAGCATGTCCTTAGTGGTTATCAAAGGTAATGTAATCTGACAAAAAATTCCCTATTTTCATATTCTGTAACTATATTCTTTCTTTTATTTCATCATAATTCTCAAATAGTCTGTGCTTACTGGTGTTAGCATGGAATGCAGCCATTGAGGGTTAAGGATTGGGATAGGGCACTGGTGGAATTCACTGCTGCAATGCTTCTGGACGAGGAATCTAATACAAAGCCAGAACTTTCTAAAAGACTGAATGAAATATCTTGTCCTGGTGATTTCACTGACCCTTAATGAAATATTTTTTTCTGTTGTGTAAAGATCTAACTGGTTTCATAGCCTCAAGTTGAACCTTGGTCATAATTGGTGATATTGTTTGACTGTGCAGTTCTGATTGTAACCGGTGATAGTGATCGACTAGTTCCTTCATGGAATGCTGAGAGGACTTTCAAGAGTCATACCAGGAGCATC

Protein sequence

>MS.gene049426.t1

MYIAKAIAEMMKPMINILNSLYKKLLSSILRSSPGIMLVRMAIDKFGAAAVRNSWYDPKQVSEHVLSGYQRVKDWDRALVEFTAAMLLDEESNTKPELSKRLNEISCPVLIVTGDSDRLVPSWNAERTFKSHTRSI