Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54221.t1 | XP_013453459.1 | 83.8 | 105 | 17 | 0 | 1 | 105 | 1 | 105 | 3.30E-35 | 157.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54221.t1 | A0A072UNS2 | 83.8 | 105 | 17 | 0 | 1 | 105 | 1 | 105 | 2.4e-35 | 157.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene54221 | 0.828942 | 6.79E-55 | -1.69E-46 |
| MS.gene049341 | MS.gene54221 | 0.809995 | 1.44E-50 | -1.69E-46 |
| MS.gene049382 | MS.gene54221 | 0.831692 | 1.44E-55 | -1.69E-46 |
| MS.gene049756 | MS.gene54221 | 0.809758 | 1.62E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene54221 | 0.800925 | 1.15E-48 | -1.69E-46 |
| MS.gene050277 | MS.gene54221 | 0.841166 | 5.60E-58 | -1.69E-46 |
| MS.gene050303 | MS.gene54221 | 0.808411 | 3.14E-50 | -1.69E-46 |
| MS.gene050586 | MS.gene54221 | 0.826627 | 2.45E-54 | -1.69E-46 |
| MS.gene050623 | MS.gene54221 | -0.809007 | 2.34E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene54221 | 0.828598 | 8.22E-55 | -1.69E-46 |
| MS.gene050815 | MS.gene54221 | 0.832247 | 1.05E-55 | -1.69E-46 |
| MS.gene050987 | MS.gene54221 | 0.822757 | 2.00E-53 | -1.69E-46 |
| MS.gene051243 | MS.gene54221 | 0.841782 | 3.86E-58 | -1.69E-46 |
| MS.gene051245 | MS.gene54221 | 0.811777 | 5.91E-51 | -1.69E-46 |
| MS.gene051328 | MS.gene54221 | 0.813489 | 2.49E-51 | -1.69E-46 |
| MS.gene051349 | MS.gene54221 | 0.812875 | 3.40E-51 | -1.69E-46 |
| MS.gene05134 | MS.gene54221 | 0.828865 | 7.09E-55 | -1.69E-46 |
| MS.gene051577 | MS.gene54221 | 0.809201 | 2.13E-50 | -1.69E-46 |
| MS.gene051633 | MS.gene54221 | 0.820484 | 6.69E-53 | -1.69E-46 |
| MS.gene051662 | MS.gene54221 | 0.822496 | 2.30E-53 | -1.69E-46 |
| MS.gene051789 | MS.gene54221 | 0.830319 | 3.14E-55 | -1.69E-46 |
| MS.gene052133 | MS.gene54221 | 0.80036 | 1.50E-48 | -1.69E-46 |
| MS.gene052286 | MS.gene54221 | 0.849425 | 3.26E-60 | -1.69E-46 |
| MS.gene052302 | MS.gene54221 | 0.858435 | 8.26E-63 | -1.69E-46 |
| MS.gene052376 | MS.gene54221 | 0.858243 | 9.42E-63 | -1.69E-46 |
| MS.gene052665 | MS.gene54221 | 0.815066 | 1.12E-51 | -1.69E-46 |
| MS.gene052856 | MS.gene54221 | 0.832838 | 7.52E-56 | -1.69E-46 |
| MS.gene052858 | MS.gene54221 | 0.824079 | 9.80E-54 | -1.69E-46 |
| MS.gene052860 | MS.gene54221 | 0.83221 | 1.08E-55 | -1.69E-46 |
| MS.gene052947 | MS.gene54221 | 0.80691 | 6.54E-50 | -1.69E-46 |
| MS.gene053109 | MS.gene54221 | 0.805195 | 1.50E-49 | -1.69E-46 |
| MS.gene053630 | MS.gene54221 | 0.814675 | 1.37E-51 | -1.69E-46 |
| MS.gene054930 | MS.gene54221 | 0.801996 | 6.92E-49 | -1.69E-46 |
| MS.gene055133 | MS.gene54221 | 0.813072 | 3.08E-51 | -1.69E-46 |
| MS.gene055207 | MS.gene54221 | 0.801 | 1.11E-48 | -1.69E-46 |
| MS.gene056069 | MS.gene54221 | -0.832659 | 8.33E-56 | -1.69E-46 |
| MS.gene056631 | MS.gene54221 | 0.826427 | 2.73E-54 | -1.69E-46 |
| MS.gene056760 | MS.gene54221 | 0.843077 | 1.75E-58 | -1.69E-46 |
| MS.gene057057 | MS.gene54221 | 0.824799 | 6.64E-54 | -1.69E-46 |
| MS.gene057079 | MS.gene54221 | 0.805241 | 1.47E-49 | -1.69E-46 |
| MS.gene057140 | MS.gene54221 | 0.823431 | 1.39E-53 | -1.69E-46 |
| MS.gene057234 | MS.gene54221 | 0.809478 | 1.86E-50 | -1.69E-46 |
| MS.gene057260 | MS.gene54221 | 0.812013 | 5.25E-51 | -1.69E-46 |
| MS.gene057439 | MS.gene54221 | 0.803825 | 2.90E-49 | -1.69E-46 |
| MS.gene058084 | MS.gene54221 | 0.814884 | 1.23E-51 | -1.69E-46 |
| MS.gene058182 | MS.gene54221 | 0.808999 | 2.35E-50 | -1.69E-46 |
| MS.gene058238 | MS.gene54221 | 0.817097 | 3.93E-52 | -1.69E-46 |
| MS.gene058906 | MS.gene54221 | 0.84412 | 9.21E-59 | -1.69E-46 |
| MS.gene058982 | MS.gene54221 | 0.802799 | 4.73E-49 | -1.69E-46 |
| MS.gene059898 | MS.gene54221 | 0.800398 | 1.47E-48 | -1.69E-46 |
| MS.gene06001 | MS.gene54221 | 0.832278 | 1.04E-55 | -1.69E-46 |
| MS.gene060476 | MS.gene54221 | 0.815706 | 8.06E-52 | -1.69E-46 |
| MS.gene060803 | MS.gene54221 | 0.807529 | 4.84E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene54221 | 0.808798 | 2.59E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54221.t1 | MTR_5g013975 | 98.095 | 105 | 2 | 0 | 1 | 105 | 1 | 105 | 9.89e-72 | 208 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54221.t1 | AT3G59840 | 58.095 | 105 | 36 | 2 | 1 | 105 | 1 | 97 | 1.58e-34 | 114 |
Find 36 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACGATAAGGTTACTCTTGC+CGG | 0.293256 | 5.1:+6845130 | MS.gene54221:CDS |
| GTCGTCGACGACTTTGCTTC+AGG | 0.338950 | 5.1:-6844968 | None:intergenic |
| GGTTCAAGATCTTCAGAAAC+TGG | 0.373697 | 5.1:-6845160 | None:intergenic |
| TTCAGAAACTGGACAATAAC+CGG | 0.394776 | 5.1:-6845149 | None:intergenic |
| ATCCCTGGCGCCTCCGTGGT+TGG | 0.398567 | 5.1:-6845031 | None:intergenic |
| AAACCAACCACGGAGGCGCC+AGG | 0.442569 | 5.1:+6845028 | MS.gene54221:CDS |
| GGTTGCTGCGAGCAACGATA+AGG | 0.463185 | 5.1:+6845117 | MS.gene54221:CDS |
| GTTTACTGTGGTGGCGGAAG+GGG | 0.469288 | 5.1:+6844933 | MS.gene54221:CDS |
| AGATCTTGAACCTTGTCGTT+GGG | 0.475907 | 5.1:+6845171 | MS.gene54221:CDS |
| AGCAAAGAATGGCGGTGCCT+CGG | 0.478420 | 5.1:+6845000 | MS.gene54221:CDS |
| TCCACTGCACCGTTCATCCC+TGG | 0.487594 | 5.1:-6845046 | None:intergenic |
| GGGTTTACTGTGGTGGCGGA+AGG | 0.492850 | 5.1:+6844931 | MS.gene54221:CDS |
| ACTGAAACACTCGCGTTCAA+AGG | 0.493969 | 5.1:-6845079 | None:intergenic |
| AAGATCTTGAACCTTGTCGT+TGG | 0.501854 | 5.1:+6845170 | MS.gene54221:CDS |
| ACGGTGCAGTGGAGGTTAAA+CGG | 0.502142 | 5.1:+6845056 | MS.gene54221:CDS |
| GGTTTACTGTGGTGGCGGAA+GGG | 0.516623 | 5.1:+6844932 | MS.gene54221:CDS |
| ACGGAGAACGCAGCAAAGAA+TGG | 0.516724 | 5.1:+6844989 | MS.gene54221:CDS |
| TAAGAATGGGTTTACTGTGG+TGG | 0.544385 | 5.1:+6844924 | None:intergenic |
| GAATGGGTTTACTGTGGTGG+CGG | 0.545194 | 5.1:+6844927 | None:intergenic |
| TTGTCGTTGGGAAATTCTGC+CGG | 0.548386 | 5.1:+6845183 | MS.gene54221:CDS |
| GGCGCGTTTGACTCAACCGC+CGG | 0.563952 | 5.1:-6845202 | None:intergenic |
| ACGGAGGCGCCAGGGATGAA+CGG | 0.590223 | 5.1:+6845037 | MS.gene54221:CDS |
| AGGGATGAACGGTGCAGTGG+AGG | 0.592078 | 5.1:+6845048 | MS.gene54221:CDS |
| GGCAGAATTTCCCAACGACA+AGG | 0.605086 | 5.1:-6845181 | None:intergenic |
| GTTCATCCCTGGCGCCTCCG+TGG | 0.608954 | 5.1:-6845035 | None:intergenic |
| AACCAACCACGGAGGCGCCA+GGG | 0.609462 | 5.1:+6845029 | MS.gene54221:CDS |
| GCCAGGGATGAACGGTGCAG+TGG | 0.624288 | 5.1:+6845045 | MS.gene54221:CDS |
| CGTGGTTGGTTTCACCGCCG+AGG | 0.625680 | 5.1:-6845017 | None:intergenic |
| CAGAAAACAACGCGAAACTG+CGG | 0.626649 | 5.1:-6845223 | None:intergenic |
| AAAGAATGGCGGTGCCTCGG+CGG | 0.629398 | 5.1:+6845003 | MS.gene54221:CDS |
| TGAAGCAAAGTCGTCGACGA+CGG | 0.647435 | 5.1:+6844970 | MS.gene54221:CDS |
| CGTTAAGAATGGGTTTACTG+TGG | 0.661274 | 5.1:+6844921 | None:intergenic |
| TCGTTGGGAAATTCTGCCGG+CGG | 0.672246 | 5.1:+6845186 | MS.gene54221:CDS |
| GAGAACGCAGCAAAGAATGG+CGG | 0.729371 | 5.1:+6844992 | MS.gene54221:CDS |
| CTCGGCGGTGAAACCAACCA+CGG | 0.734856 | 5.1:+6845018 | MS.gene54221:CDS |
| GGCGGTGAAACCAACCACGG+AGG | 0.756389 | 5.1:+6845021 | MS.gene54221:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTCAGAAACTGGACAATAAC+CGG | - | chr5.1:6845152-6845171 | None:intergenic | 35.0% | |
| !!! | AGTGTTTCAGTTTTCGAGTT+TGG | + | chr5.1:6845091-6845110 | MS.gene54221:CDS | 35.0% |
| !!! | TTCAGTTTTCGAGTTTGGTT+CGG | + | chr5.1:6845096-6845115 | MS.gene54221:CDS | 35.0% |
| AACGATAAGGTTACTCTTGC+CGG | + | chr5.1:6845130-6845149 | MS.gene54221:CDS | 40.0% | |
| AAGATCTTGAACCTTGTCGT+TGG | + | chr5.1:6845170-6845189 | MS.gene54221:CDS | 40.0% | |
| AGATCTTGAACCTTGTCGTT+GGG | + | chr5.1:6845171-6845190 | MS.gene54221:CDS | 40.0% | |
| GGTTCAAGATCTTCAGAAAC+TGG | - | chr5.1:6845163-6845182 | None:intergenic | 40.0% | |
| ACTGAAACACTCGCGTTCAA+AGG | - | chr5.1:6845082-6845101 | None:intergenic | 45.0% | |
| CAGAAAACAACGCGAAACTG+CGG | - | chr5.1:6845226-6845245 | None:intergenic | 45.0% | |
| TTGTCGTTGGGAAATTCTGC+CGG | + | chr5.1:6845183-6845202 | MS.gene54221:CDS | 45.0% | |
| ACGGAGAACGCAGCAAAGAA+TGG | + | chr5.1:6844989-6845008 | MS.gene54221:CDS | 50.0% | |
| ACGGTGCAGTGGAGGTTAAA+CGG | + | chr5.1:6845056-6845075 | MS.gene54221:CDS | 50.0% | |
| GAGAACGCAGCAAAGAATGG+CGG | + | chr5.1:6844992-6845011 | MS.gene54221:CDS | 50.0% | |
| GGCAGAATTTCCCAACGACA+AGG | - | chr5.1:6845184-6845203 | None:intergenic | 50.0% | |
| TGAAGCAAAGTCGTCGACGA+CGG | + | chr5.1:6844970-6844989 | MS.gene54221:CDS | 50.0% | |
| AGCAAAGAATGGCGGTGCCT+CGG | + | chr5.1:6845000-6845019 | MS.gene54221:CDS | 55.0% | |
| GGTTGCTGCGAGCAACGATA+AGG | + | chr5.1:6845117-6845136 | MS.gene54221:CDS | 55.0% | |
| GTCGTCGACGACTTTGCTTC+AGG | - | chr5.1:6844971-6844990 | None:intergenic | 55.0% | |
| TCGTTGGGAAATTCTGCCGG+CGG | + | chr5.1:6845186-6845205 | MS.gene54221:CDS | 55.0% | |
| !! | GGTTTACTGTGGTGGCGGAA+GGG | + | chr5.1:6844932-6844951 | MS.gene54221:CDS | 55.0% |
| !! | GTTTACTGTGGTGGCGGAAG+GGG | + | chr5.1:6844933-6844952 | MS.gene54221:CDS | 55.0% |
| AAAGAATGGCGGTGCCTCGG+CGG | + | chr5.1:6845003-6845022 | MS.gene54221:CDS | 60.0% | |
| AGGGATGAACGGTGCAGTGG+AGG | + | chr5.1:6845048-6845067 | MS.gene54221:CDS | 60.0% | |
| CTCGGCGGTGAAACCAACCA+CGG | + | chr5.1:6845018-6845037 | MS.gene54221:CDS | 60.0% | |
| TCCACTGCACCGTTCATCCC+TGG | - | chr5.1:6845049-6845068 | None:intergenic | 60.0% | |
| !! | GGGTTTACTGTGGTGGCGGA+AGG | + | chr5.1:6844931-6844950 | MS.gene54221:CDS | 60.0% |
| GCCAGGGATGAACGGTGCAG+TGG | + | chr5.1:6845045-6845064 | MS.gene54221:CDS | 65.0% | |
| GGCGCGTTTGACTCAACCGC+CGG | - | chr5.1:6845205-6845224 | None:intergenic | 65.0% | |
| GGCGGTGAAACCAACCACGG+AGG | + | chr5.1:6845021-6845040 | MS.gene54221:CDS | 65.0% | |
| !! | AAACCAACCACGGAGGCGCC+AGG | + | chr5.1:6845028-6845047 | MS.gene54221:CDS | 65.0% |
| !! | AACCAACCACGGAGGCGCCA+GGG | + | chr5.1:6845029-6845048 | MS.gene54221:CDS | 65.0% |
| !! | ACGGAGGCGCCAGGGATGAA+CGG | + | chr5.1:6845037-6845056 | MS.gene54221:CDS | 65.0% |
| !! | CGTGGTTGGTTTCACCGCCG+AGG | - | chr5.1:6845020-6845039 | None:intergenic | 65.0% |
| ATCCCTGGCGCCTCCGTGGT+TGG | - | chr5.1:6845034-6845053 | None:intergenic | 70.0% | |
| GTTCATCCCTGGCGCCTCCG+TGG | - | chr5.1:6845038-6845057 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 6844929 | 6845246 | 6844929 | ID=MS.gene54221 |
| chr5.1 | mRNA | 6844929 | 6845246 | 6844929 | ID=MS.gene54221.t1;Parent=MS.gene54221 |
| chr5.1 | exon | 6844929 | 6845246 | 6844929 | ID=MS.gene54221.t1.exon1;Parent=MS.gene54221.t1 |
| chr5.1 | CDS | 6844929 | 6845246 | 6844929 | ID=cds.MS.gene54221.t1;Parent=MS.gene54221.t1 |
| Gene Sequence |
| Protein sequence |
