AlfalfaGEDB | MS.gene57951

M. sativa cultivar XinJiangDaYe / MS.gene57951

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57951.t1	XP_003610246.2	94.5	181	6	2	1	181	1	177	1.00E-79	306.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57951.t1	Q9M0B9	38.6	184	105	5	1	180	1	180	6.0e-17	89.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57951.t1	G7JEC9	94.5	181	6	2	1	181	1	177	7.2e-80	306.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049230	MS.gene57951	0.839164	1.87E-57	-1.69E-46
MS.gene049378	MS.gene57951	0.86346	2.45E-64	-1.69E-46
MS.gene049910	MS.gene57951	0.835555	1.57E-56	-1.69E-46
MS.gene050226	MS.gene57951	0.849145	3.90E-60	-1.69E-46
MS.gene050227	MS.gene57951	0.851279	9.84E-61	-1.69E-46
MS.gene050228	MS.gene57951	0.858318	8.94E-63	-1.69E-46
MS.gene050623	MS.gene57951	-0.834655	2.64E-56	-1.69E-46
MS.gene050642	MS.gene57951	0.857362	1.72E-62	-1.69E-46
MS.gene050680	MS.gene57951	0.820078	8.30E-53	-1.69E-46
MS.gene050750	MS.gene57951	0.806322	8.70E-50	-1.69E-46
MS.gene050820	MS.gene57951	0.831827	1.34E-55	-1.69E-46
MS.gene050953	MS.gene57951	0.82532	5.00E-54	-1.69E-46
MS.gene05119	MS.gene57951	-0.820401	6.99E-53	-1.69E-46
MS.gene051370	MS.gene57951	0.80775	4.34E-50	-1.69E-46
MS.gene051373	MS.gene57951	0.81858	1.82E-52	-1.69E-46
MS.gene05137	MS.gene57951	0.808572	2.90E-50	-1.69E-46
MS.gene051535	MS.gene57951	0.813778	2.15E-51	-1.69E-46
MS.gene051983	MS.gene57951	0.880157	6.92E-70	-1.69E-46
MS.gene052077	MS.gene57951	0.841329	5.08E-58	-1.69E-46
MS.gene052176	MS.gene57951	0.830675	2.57E-55	-1.69E-46
MS.gene052302	MS.gene57951	0.804325	2.28E-49	-1.69E-46
MS.gene052309	MS.gene57951	-0.854203	1.44E-61	-1.69E-46
MS.gene052333	MS.gene57951	0.800409	1.46E-48	-1.69E-46
MS.gene052376	MS.gene57951	0.824369	8.38E-54	-1.69E-46
MS.gene052426	MS.gene57951	0.824081	9.79E-54	-1.69E-46
MS.gene052586	MS.gene57951	0.808117	3.63E-50	-1.69E-46
MS.gene053494	MS.gene57951	0.80724	5.57E-50	-1.69E-46
MS.gene053836	MS.gene57951	0.841773	3.88E-58	-1.69E-46
MS.gene054405	MS.gene57951	0.814032	1.89E-51	-1.69E-46
MS.gene054930	MS.gene57951	0.876732	1.11E-68	-1.69E-46
MS.gene054983	MS.gene57951	0.826759	2.27E-54	-1.69E-46
MS.gene05510	MS.gene57951	0.817829	2.69E-52	-1.69E-46
MS.gene055367	MS.gene57951	0.827659	1.38E-54	-1.69E-46
MS.gene05553	MS.gene57951	0.8117	6.14E-51	-1.69E-46
MS.gene055701	MS.gene57951	0.874117	8.68E-68	-1.69E-46
MS.gene056506	MS.gene57951	0.801973	6.99E-49	-1.69E-46
MS.gene056723	MS.gene57951	0.802117	6.53E-49	-1.69E-46
MS.gene056979	MS.gene57951	0.874	9.50E-68	-1.69E-46
MS.gene05761	MS.gene57951	0.817528	3.15E-52	-1.69E-46
MS.gene057639	MS.gene57951	0.807137	5.85E-50	-1.69E-46
MS.gene057750	MS.gene57951	0.812625	3.86E-51	-1.69E-46
MS.gene058142	MS.gene57951	0.82599	3.47E-54	-1.69E-46
MS.gene058146	MS.gene57951	0.801798	7.60E-49	-1.69E-46
MS.gene058435	MS.gene57951	0.837342	5.50E-57	-1.69E-46
MS.gene058749	MS.gene57951	0.807519	4.86E-50	-1.69E-46
MS.gene059247	MS.gene57951	0.803247	3.82E-49	-1.69E-46
MS.gene059285	MS.gene57951	0.828049	1.12E-54	-1.69E-46
MS.gene059511	MS.gene57951	0.821412	4.09E-53	-1.69E-46
MS.gene059894	MS.gene57951	0.804585	2.01E-49	-1.69E-46
MS.gene059899	MS.gene57951	0.816935	4.28E-52	-1.69E-46
MS.gene060297	MS.gene57951	0.802505	5.43E-49	-1.69E-46
MS.gene060683	MS.gene57951	0.800355	1.50E-48	-1.69E-46
MS.gene060879	MS.gene57951	0.820847	5.52E-53	-1.69E-46
MS.gene061040	MS.gene57951	-0.822509	2.28E-53	-1.69E-46
MS.gene061049	MS.gene57951	0.806844	6.75E-50	-1.69E-46
MS.gene061114	MS.gene57951	-0.840516	8.30E-58	-1.69E-46
MS.gene061159	MS.gene57951	0.816383	5.69E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57951.t1	MTR_4g129560	94.475	181	6	2	1	181	1	177	4.01e-118	332

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57951.t1	AT4G30410	42.391	184	98	5	1	180	1	180	1.40e-30	110
MS.gene57951.t1	AT4G30410	42.391	184	98	5	1	180	1	180	1.40e-30	110
MS.gene57951.t1	AT5G57780	38.764	178	85	6	1	177	2	156	1.54e-28	104

Find 25 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTATTCTCTTTATAGAAAA+AGG	0.151336	8.4:+5259525	MS.gene57951:CDS
TAAATCAGCAGAAAGCTTTA+TGG	0.212678	8.4:-5259360	None:intergenic
TCTTCACATAAGAAGGTAAT+AGG	0.223742	8.4:+5259485	MS.gene57951:CDS
TTGTTGAAGTATTTGCAATT+AGG	0.256685	8.4:-5259430	None:intergenic
TGAGAGATTCTATATAATCT+AGG	0.292570	8.4:-5259706	None:intergenic
TGTTGAAGTATTTGCAATTA+GGG	0.303984	8.4:-5259429	None:intergenic
ATTCCTAAGGAAATGGATAA+AGG	0.331543	8.4:+5259277	MS.gene57951:CDS
GTTACCAGGTGGAAAATTCA+TGG	0.380790	8.4:+5259658	MS.gene57951:CDS
AGCAAGAATTCCTAAGGAAA+TGG	0.389199	8.4:+5259270	MS.gene57951:CDS
CTTCACATAAGAAGGTAATA+GGG	0.431570	8.4:+5259486	MS.gene57951:CDS
TTCCTAAGGAAATGGATAAA+GGG	0.440057	8.4:+5259278	MS.gene57951:CDS
CTAAAGACAAAACTACCCTT+TGG	0.442557	8.4:+5259399	MS.gene57951:CDS
GACCCTTTATCCATTTCCTT+AGG	0.455134	8.4:-5259280	None:intergenic
GAGAGATTCTATATAATCTA+GGG	0.474006	8.4:-5259705	None:intergenic
GTCTTTAGACGTGGAAGCCA+TGG	0.491623	8.4:-5259384	None:intergenic
AACCTCATCTTCACATAAGA+AGG	0.491845	8.4:+5259478	MS.gene57951:CDS
TGCTCAAGCAAGAATTCCTA+AGG	0.547032	8.4:+5259264	MS.gene57951:CDS
TACCTTCTTATGTGAAGATG+AGG	0.560965	8.4:-5259480	None:intergenic
GCTTTCTGCTGATTTAGCCA+TGG	0.566451	8.4:+5259367	MS.gene57951:CDS
CTCAAGAGTTTGTTACCAGG+TGG	0.612619	8.4:+5259647	MS.gene57951:CDS
AGGCTCAAGAGTTTGTTACC+AGG	0.621754	8.4:+5259644	MS.gene57951:CDS
TGGTTAAGAAAAGAACAAGA+AGG	0.644687	8.4:+5259624	MS.gene57951:CDS
TCGATTAACGAAGAGTTCTG+AGG	0.651928	8.4:-5259765	None:intergenic
GTTGAGAAGAAATCGTGCAA+AGG	0.662740	8.4:+5259568	MS.gene57951:CDS
TCGTGCAAAGGAAAGAGTTG+AGG	0.666359	8.4:+5259580	MS.gene57951:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTTTATAGAAAAAGGATAAT+TGG	+	chr8.4:5259533-5259552	MS.gene57951:CDS	20.0%
!	ATAGAAAAAGGATAATTGGA+AGG	+	chr8.4:5259537-5259556	MS.gene57951:CDS	25.0%
!	CCTATTCTCTTTATAGAAAA+AGG	+	chr8.4:5259525-5259544	MS.gene57951:CDS	25.0%
!	GAGAGATTCTATATAATCTA+GGG	-	chr8.4:5259708-5259727	None:intergenic	25.0%
!	TGAGAGATTCTATATAATCT+AGG	-	chr8.4:5259709-5259728	None:intergenic	25.0%
!!	CCTTTTTCTATAAAGAGAAT+AGG	-	chr8.4:5259528-5259547	None:intergenic	25.0%
!!	TGTTGAAGTATTTGCAATTA+GGG	-	chr8.4:5259432-5259451	None:intergenic	25.0%
!!	TTGTTGAAGTATTTGCAATT+AGG	-	chr8.4:5259433-5259452	None:intergenic	25.0%
	ATTCCTAAGGAAATGGATAA+AGG	+	chr8.4:5259277-5259296	MS.gene57951:CDS	30.0%
	CTTCACATAAGAAGGTAATA+GGG	+	chr8.4:5259486-5259505	MS.gene57951:CDS	30.0%
	TAAATCAGCAGAAAGCTTTA+TGG	-	chr8.4:5259363-5259382	None:intergenic	30.0%
	TCTTCACATAAGAAGGTAAT+AGG	+	chr8.4:5259485-5259504	MS.gene57951:CDS	30.0%
	TGGTTAAGAAAAGAACAAGA+AGG	+	chr8.4:5259624-5259643	MS.gene57951:CDS	30.0%
	TTCCTAAGGAAATGGATAAA+GGG	+	chr8.4:5259278-5259297	MS.gene57951:CDS	30.0%
	AACCTCATCTTCACATAAGA+AGG	+	chr8.4:5259478-5259497	MS.gene57951:CDS	35.0%
	AGCAAGAATTCCTAAGGAAA+TGG	+	chr8.4:5259270-5259289	MS.gene57951:CDS	35.0%
	CTAAAGACAAAACTACCCTT+TGG	+	chr8.4:5259399-5259418	MS.gene57951:CDS	35.0%
	TACCTTCTTATGTGAAGATG+AGG	-	chr8.4:5259483-5259502	None:intergenic	35.0%
!	AATTAGGGCTTTTCTCCAAA+GGG	-	chr8.4:5259417-5259436	None:intergenic	35.0%
	GACCCTTTATCCATTTCCTT+AGG	-	chr8.4:5259283-5259302	None:intergenic	40.0%
	GTTACCAGGTGGAAAATTCA+TGG	+	chr8.4:5259658-5259677	MS.gene57951:CDS	40.0%
	GTTGAGAAGAAATCGTGCAA+AGG	+	chr8.4:5259568-5259587	MS.gene57951:CDS	40.0%
	TCGATTAACGAAGAGTTCTG+AGG	-	chr8.4:5259768-5259787	None:intergenic	40.0%
	TGCTCAAGCAAGAATTCCTA+AGG	+	chr8.4:5259264-5259283	MS.gene57951:CDS	40.0%
!	CAATTAGGGCTTTTCTCCAA+AGG	-	chr8.4:5259418-5259437	None:intergenic	40.0%
!	TCATCCATGAATTTTCCACC+TGG	-	chr8.4:5259665-5259684	None:intergenic	40.0%
!!!	GGTAGTTTTGTCTTTAGACG+TGG	-	chr8.4:5259396-5259415	None:intergenic	40.0%
	TCGTGCAAAGGAAAGAGTTG+AGG	+	chr8.4:5259580-5259599	MS.gene57951:CDS	45.0%
!!	AGGCGAGTTTTATAGCGACA+CGG	+	chr8.4:5259600-5259619	MS.gene57951:CDS	45.0%
!!	AGGCTCAAGAGTTTGTTACC+AGG	+	chr8.4:5259644-5259663	MS.gene57951:CDS	45.0%
!!	CTCAAGAGTTTGTTACCAGG+TGG	+	chr8.4:5259647-5259666	MS.gene57951:CDS	45.0%
!!	GAGTTTTATAGCGACACGGT+TGG	+	chr8.4:5259604-5259623	MS.gene57951:CDS	45.0%
!!	GCTTTCTGCTGATTTAGCCA+TGG	+	chr8.4:5259367-5259386	MS.gene57951:CDS	45.0%
	GTCTTTAGACGTGGAAGCCA+TGG	-	chr8.4:5259387-5259406	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	5259248	5259793	5259248	ID=MS.gene57951
chr8.4	mRNA	5259248	5259793	5259248	ID=MS.gene57951.t1;Parent=MS.gene57951
chr8.4	exon	5259248	5259793	5259248	ID=MS.gene57951.t1.exon1;Parent=MS.gene57951.t1
chr8.4	CDS	5259248	5259793	5259248	ID=cds.MS.gene57951.t1;Parent=MS.gene57951.t1

Gene Sequence

>MS.gene57951

ATGCGAAATTTGAGCTTGCTCAAGCAAGAATTCCTAAGGAAATGGATAAAGGGTCTAAAAAAATATTATAACTCTCAAAAGAAGAATATGAACTTGTTCGAGAGAAAGAAAGCCATAAAGCTTTCTGCTGATTTAGCCATGGCTTCCACGTCTAAAGACAAAACTACCCTTTGGAGAAAAGCCCTAATTGCAAATACTTCAACAAGCATAGATGAACAAGACATATCAACAACCTCATCTTCACATAAGAAGGTAATAGGGAAAAACTCGACGAATTCCTATTCTCTTTATAGAAAAAGGATAATTGGAAGGAGAAAGACGTTGAGAAGAAATCGTGCAAAGGAAAGAGTTGAGGCGAGTTTTATAGCGACACGGTTGGTTAAGAAAAGAACAAGAAGGCTCAAGAGTTTGTTACCAGGTGGAAAATTCATGGATGATGCTTGTTTAGTTGATGAAACCCTAGATTATATAGAATCTCTCAAAGCTCAAGTTGAAGTGATGAGATGTCTTGTTACTGCCTCAGAACTCTTCGTTAATCGACCGTAA

Protein sequence

>MS.gene57951.t1

MRNLSLLKQEFLRKWIKGLKKYYNSQKKNMNLFERKKAIKLSADLAMASTSKDKTTLWRKALIANTSTSIDEQDISTTSSSHKKVIGKNSTNSYSLYRKRIIGRRKTLRRNRAKERVEASFIATRLVKKRTRRLKSLLPGGKFMDDACLVDETLDYIESLKAQVEVMRCLVTASELFVNRP