AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58248


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58248.t1 MTR_8g032320 95.946 148 6 0 1 148 1 148 3.88e-103 291
MS.gene58248.t1 MTR_8g032300 83.784 148 24 0 1 148 1 148 8.89e-93 265
MS.gene58248.t1 MTR_1g008950 63.200 125 46 0 1 125 1 125 2.65e-57 175
MS.gene58248.t1 MTR_5g079880 40.645 155 77 4 1 144 1 151 6.88e-37 124
MS.gene58248.t1 MTR_5g079890 40.506 158 78 5 1 145 1 155 1.09e-33 116
MS.gene58248.t1 MTR_5g079890 40.580 138 69 4 1 125 1 138 9.07e-30 105
MS.gene58248.t1 MTR_8g030870 33.108 148 85 4 1 135 1 147 2.95e-20 82.4
MS.gene58248.t1 MTR_7g075960 56.604 53 23 0 25 77 1 53 1.85e-19 77.0
MS.gene58248.t1 MTR_5g004800 26.984 126 90 2 6 130 15 139 1.06e-13 66.2
MS.gene58248.t1 MTR_3g106660 33.333 126 78 4 3 126 439 560 5.95e-13 65.5
MS.gene58248.t1 MTR_3g106660 30.303 132 82 4 2 124 255 385 4.77e-11 59.7
MS.gene58248.t1 MTR_2g027100 38.542 96 57 2 4 99 6 99 6.12e-13 62.8
MS.gene58248.t1 MTR_5g004870 28.571 119 83 2 6 123 11 128 9.60e-13 63.9
MS.gene58248.t1 MTR_4g115660 36.275 102 62 3 3 102 440 540 6.95e-11 59.3
MS.gene58248.t1 MTR_3g017500 33.000 100 65 2 4 103 244 341 7.99e-11 58.9
MS.gene58248.t1 MTR_3g017500 33.000 100 65 2 4 103 214 311 8.11e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58248.t1 AT1G63220 56.757 148 63 1 1 148 1 147 3.61e-56 172
MS.gene58248.t1 AT1G63220 52.703 148 55 2 1 148 1 133 4.46e-47 149
MS.gene58248.t1 AT3G55470 37.342 158 85 4 1 147 1 155 6.57e-27 99.0
MS.gene58248.t1 AT3G55470 37.736 159 84 5 1 147 1 156 7.05e-26 96.3
MS.gene58248.t1 AT4G00467 33.083 133 78 3 1 122 1 133 7.53e-18 76.3
MS.gene58248.t1 AT4G00467 33.083 133 78 3 1 122 1 133 1.23e-17 76.3
MS.gene58248.t1 AT5G47710 38.776 98 58 2 2 99 4 99 6.81e-14 65.5
MS.gene58248.t1 AT5G47710 38.776 98 58 2 2 99 4 99 6.81e-14 65.5
MS.gene58248.t1 AT5G47710 38.776 98 58 2 2 99 4 99 6.81e-14 65.5
MS.gene58248.t1 AT5G47710 38.776 98 58 2 2 99 4 99 6.81e-14 65.5
MS.gene58248.t1 AT4G34150 27.966 118 83 2 6 122 12 128 2.81e-13 65.1
MS.gene58248.t1 AT5G04220 29.452 146 90 4 2 135 37 181 2.65e-12 63.2
MS.gene58248.t1 AT5G04220 29.452 146 90 4 2 135 197 341 3.55e-12 63.2
MS.gene58248.t1 AT5G04220 29.054 148 92 4 2 137 259 405 4.29e-12 62.8
MS.gene58248.t1 AT1G05500 35.200 125 75 5 3 125 290 410 2.77e-11 60.5
MS.gene58248.t1 AT1G05500 35.200 125 75 5 3 125 434 554 3.63e-11 60.1
MS.gene58248.t1 AT1G03370 33.065 124 76 3 6 123 3 125 5.05e-11 60.1
MS.gene58248.t1 AT1G03370 33.065 124 76 3 6 123 3 125 6.38e-11 59.7

Find 26 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAATAATGATGATAATAT+TGG 0.281672 8.4:+64381428 MS.gene58248:CDS
AGAATATTGTGGAGAGATTA+AGG 0.301540 8.4:+64381980 MS.gene58248:CDS
TGAGCTGAATCTAAAGATAA+TGG 0.346296 8.4:+64381394 MS.gene58248:CDS
ACAAGATTGTAAGAATTCTC+TGG 0.392769 8.4:-64381948 None:intergenic
GCTCCTGTGCTTTGTAACTA+AGG 0.446848 8.4:-64381195 None:intergenic
GAAAAGTCTCATTCCATTGT+GGG 0.465952 8.4:-64381346 None:intergenic
GAATCTAAAGATAATGGAAA+AGG 0.493781 8.4:+64381400 MS.gene58248:CDS
TCTTCAAACACTGCTTCTAA+AGG 0.503608 8.4:-64381915 None:intergenic
AAGAGGAGGAGACTGGTGGA+TGG 0.524498 8.4:+64382150 MS.gene58248:CDS
TACAATGAAGAGGAGGAGAC+TGG 0.545609 8.4:+64382143 MS.gene58248:CDS
AGAAAAGTCTCATTCCATTG+TGG 0.555092 8.4:-64381347 None:intergenic
TTGTAACTAAGGATCACATA+AGG 0.555757 8.4:-64381184 None:intergenic
CACAAGAGTAAAGTTCAAGA+AGG 0.561261 8.4:+64381217 MS.gene58248:CDS
TTAGAAGCAGTGTTTGAAGA+GGG 0.587548 8.4:+64381918 MS.gene58248:CDS
GTGAAGGATGAAGAATATTG+TGG 0.592520 8.4:+64381969 MS.gene58248:CDS
GATCCTTAGTTACAAAGCAC+AGG 0.599886 8.4:+64381192 MS.gene58248:CDS
TGATCAGAGTTACAATGAAG+AGG 0.605643 8.4:+64382133 MS.gene58248:CDS
TTTAGAAGCAGTGTTTGAAG+AGG 0.610475 8.4:+64381917 MS.gene58248:CDS
GAATTCTTACAATCTTGTGA+AGG 0.613624 8.4:+64381953 MS.gene58248:CDS
TCAGAGTTACAATGAAGAGG+AGG 0.621822 8.4:+64382136 MS.gene58248:CDS
AATGAAGAGGAGGAGACTGG+TGG 0.640663 8.4:+64382146 MS.gene58248:CDS
GATTGTAAGAATTCTCTGGA+AGG 0.642476 8.4:-64381944 None:intergenic
AATCAAAGAAAGATGCCTCG+TGG 0.675663 8.4:+64380678 None:intergenic
GTGCTGGATCTAACCCACAA+TGG 0.679425 8.4:+64381333 MS.gene58248:CDS
ATATTGTGGAGAGATTAAGG+TGG 0.788819 8.4:+64381983 MS.gene58248:CDS
ATAACTTCAAGAGTTCCACG+AGG 0.830018 8.4:-64380693 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATAATGATGATAATAT+TGG + chr8.4:64381428-64381447 MS.gene58248:CDS 10.0%
!! AACAATTTAATTAGTTAAGT+TGG - chr8.4:64381109-64381128 None:intergenic 15.0%
!! TAAAATGTACTATCAATTAT+TGG + chr8.4:64382096-64382115 MS.gene58248:intron 15.0%
!! TAATGAAATATAAACAAAAG+TGG - chr8.4:64381656-64381675 None:intergenic 15.0%
!!! AATTGATAGTACATTTTAAT+AGG - chr8.4:64382094-64382113 None:intergenic 15.0%
!!! ATTGATAGTACATTTTAATA+GGG - chr8.4:64382093-64382112 None:intergenic 15.0%
!!! CAATAATCAAATTATTTTTC+AGG - chr8.4:64380905-64380924 None:intergenic 15.0%
!!! CTTTTGTTTATATTTCATTA+TGG + chr8.4:64381656-64381675 MS.gene58248:intron 15.0%
!!! TATCTTTTTATGAATAAATC+AGG + chr8.4:64381311-64381330 MS.gene58248:intron 15.0%
!! AGAAATTATTCATTCTAAGT+TGG + chr8.4:64380931-64380950 MS.gene58248:intron 20.0%
!! GTTTAAAAATTTACACCTAA+AGG + chr8.4:64381735-64381754 MS.gene58248:intron 20.0%
!! TGAAATATAAGCAAAAAAGT+AGG + chr8.4:64381583-64381602 MS.gene58248:intron 20.0%
!!! AAACATAAGTAGTATTGATT+TGG + chr8.4:64381252-64381271 MS.gene58248:intron 20.0%
!!! TTTTTGCTTATATTTCATTC+TGG - chr8.4:64381580-64381599 None:intergenic 20.0%
! AAAAATTTACACCTAAAGGA+GGG + chr8.4:64381739-64381758 MS.gene58248:intron 25.0%
! AAAATTGAAAGTCAAACTCT+TGG - chr8.4:64380862-64380881 None:intergenic 25.0%
! GAAATATTTGCATGAAACAA+TGG + chr8.4:64380834-64380853 MS.gene58248:intron 25.0%
! GTAATTCTATGTCTATACAT+AGG + chr8.4:64380761-64380780 MS.gene58248:intron 25.0%
! TAAAAATTTACACCTAAAGG+AGG + chr8.4:64381738-64381757 MS.gene58248:intron 25.0%
!! TGTCTTTGATTCATCTTTTT+AGG + chr8.4:64381491-64381510 MS.gene58248:intron 25.0%
!!! AGAGTTTGACTTTCAATTTT+TGG + chr8.4:64380862-64380881 MS.gene58248:intron 25.0%
!!! GAATCTAAAGATAATGGAAA+AGG + chr8.4:64381400-64381419 MS.gene58248:CDS 25.0%
!!! TTTGTTATTTTGTTCTCTAG+TGG + chr8.4:64381278-64381297 MS.gene58248:intron 25.0%
ACAAGATTGTAAGAATTCTC+TGG - chr8.4:64381951-64381970 None:intergenic 30.0%
AGAATATTGTGGAGAGATTA+AGG + chr8.4:64381980-64381999 MS.gene58248:CDS 30.0%
GAATTCTTACAATCTTGTGA+AGG + chr8.4:64381953-64381972 MS.gene58248:CDS 30.0%
GAGAAAGTAAGTGTTAAAGT+TGG + chr8.4:64381678-64381697 MS.gene58248:intron 30.0%
GTTTGACTATGTAACTTCAA+AGG - chr8.4:64381829-64381848 None:intergenic 30.0%
TGAGCTGAATCTAAAGATAA+TGG + chr8.4:64381394-64381413 MS.gene58248:CDS 30.0%
TTGCTTATATTTCATTCTGG+AGG - chr8.4:64381577-64381596 None:intergenic 30.0%
TTGTAACTAAGGATCACATA+AGG - chr8.4:64381187-64381206 None:intergenic 30.0%
TTTATGAATAAATCAGGTGC+TGG + chr8.4:64381317-64381336 MS.gene58248:intron 30.0%
!! TTATATAGCCTTATTACCTC+AGG - chr8.4:64382023-64382042 None:intergenic 30.0%
!!! GTTATTTTGATCAGTGCTAA+AGG + chr8.4:64380711-64380730 MS.gene58248:CDS 30.0%
AAAATTGAACGAGATGTCAG+TGG - chr8.4:64381857-64381876 None:intergenic 35.0%
AGAAAAGTCTCATTCCATTG+TGG - chr8.4:64381350-64381369 None:intergenic 35.0%
ATATTGTGGAGAGATTAAGG+TGG + chr8.4:64381983-64382002 MS.gene58248:CDS 35.0%
CACAAGAGTAAAGTTCAAGA+AGG + chr8.4:64381217-64381236 MS.gene58248:CDS 35.0%
GAAAAGTCTCATTCCATTGT+GGG - chr8.4:64381349-64381368 None:intergenic 35.0%
GATTGTAAGAATTCTCTGGA+AGG - chr8.4:64381947-64381966 None:intergenic 35.0%
GTGAAGGATGAAGAATATTG+TGG + chr8.4:64381969-64381988 MS.gene58248:CDS 35.0%
GTTGGATTCTCATTGAAAGT+TGG + chr8.4:64381696-64381715 MS.gene58248:intron 35.0%
TCTTCAAACACTGCTTCTAA+AGG - chr8.4:64381918-64381937 None:intergenic 35.0%
TGATCAGAGTTACAATGAAG+AGG + chr8.4:64382133-64382152 MS.gene58248:CDS 35.0%
TTAGAAGCAGTGTTTGAAGA+GGG + chr8.4:64381918-64381937 MS.gene58248:CDS 35.0%
TTTAGAAGCAGTGTTTGAAG+AGG + chr8.4:64381917-64381936 MS.gene58248:CDS 35.0%
! CTTTTACACCTGAGGTAATA+AGG + chr8.4:64382012-64382031 MS.gene58248:intron 35.0%
ATAACTTCAAGAGTTCCACG+AGG - chr8.4:64380696-64380715 None:intergenic 40.0%
GATCCTTAGTTACAAAGCAC+AGG + chr8.4:64381192-64381211 MS.gene58248:CDS 40.0%
TCAGAGTTACAATGAAGAGG+AGG + chr8.4:64382136-64382155 MS.gene58248:CDS 40.0%
AGTTGCAAAGTCGACGTATG+TGG - chr8.4:64381632-64381651 None:intergenic 45.0%
GCTCCTGTGCTTTGTAACTA+AGG - chr8.4:64381198-64381217 None:intergenic 45.0%
TACAATGAAGAGGAGGAGAC+TGG + chr8.4:64382143-64382162 MS.gene58248:CDS 45.0%
TTTCCTTTGTGCCCTCCTTT+AGG - chr8.4:64381753-64381772 None:intergenic 45.0%
AATGAAGAGGAGGAGACTGG+TGG + chr8.4:64382146-64382165 MS.gene58248:CDS 50.0%
GTGCTGGATCTAACCCACAA+TGG + chr8.4:64381333-64381352 MS.gene58248:CDS 50.0%
! ACACCTAAAGGAGGGCACAA+AGG + chr8.4:64381747-64381766 MS.gene58248:intron 50.0%
!! GGCTCTCACTTTTACACCTG+AGG + chr8.4:64382004-64382023 MS.gene58248:CDS 50.0%
AAGAGGAGGAGACTGGTGGA+TGG + chr8.4:64382150-64382169 MS.gene58248:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 64380690 64382196 64380690 ID=MS.gene58248
chr8.4 mRNA 64380690 64382196 64380690 ID=MS.gene58248.t1;Parent=MS.gene58248
chr8.4 exon 64380690 64380756 64380690 ID=MS.gene58248.t1.exon1;Parent=MS.gene58248.t1
chr8.4 CDS 64380690 64380756 64380690 ID=cds.MS.gene58248.t1;Parent=MS.gene58248.t1
chr8.4 exon 64381173 64381238 64381173 ID=MS.gene58248.t1.exon2;Parent=MS.gene58248.t1
chr8.4 CDS 64381173 64381238 64381173 ID=cds.MS.gene58248.t1;Parent=MS.gene58248.t1
chr8.4 exon 64381333 64381459 64381333 ID=MS.gene58248.t1.exon3;Parent=MS.gene58248.t1
chr8.4 CDS 64381333 64381459 64381333 ID=cds.MS.gene58248.t1;Parent=MS.gene58248.t1
chr8.4 exon 64381911 64382025 64381911 ID=MS.gene58248.t1.exon4;Parent=MS.gene58248.t1
chr8.4 CDS 64381911 64382025 64381911 ID=cds.MS.gene58248.t1;Parent=MS.gene58248.t1
chr8.4 exon 64382125 64382196 64382125 ID=MS.gene58248.t1.exon5;Parent=MS.gene58248.t1
chr8.4 CDS 64382125 64382196 64382125 ID=cds.MS.gene58248.t1;Parent=MS.gene58248.t1
Gene Sequence

>MS.gene58248

ATGCCTCGTGGAACTCTTGAAGTTATTTTGATCAGTGCTAAAGGTCTTGAAGACAATGATTTTCTATGTAAGTAATTCTATGTCTATACATAGGAGATGTGAAATTCATGTATTGTATATGTTGATGATGTTGTGTTTTTCATTGAAATATTTGCATGAAACAATGGATCCAAGAGTTTGACTTTCAATTTTTGGATAAAAGTTCATCATGACCTGAAAAATAATTTGATTATTGAACTAGAGAAATTATTCATTCTAAGTTGGTCTGATCTACATATTTATTATTCATAAAGCTTGTTAAATAAGTATATTTATCTTTTCTAATTGAATTAGAAAATCAATTTAGTCAACTTTTTCTTTGTTAATTTCTTTGCTTATAAATTGTTATAACTATAATAACATAATCTTGAAAATTACCAACTTAACTAATTAAATTGTTCAAGATGATTCTCTAATGTTCTGATCTTTGTTCTGTATGAGAAGCTAGCATAGATCCTTATGTGATCCTTAGTTACAAAGCACAGGAGCACAAGAGTAAAGTTCAAGAAGGTAAAATTCTGACAAACATAAGTAGTATTGATTTGGTTATTTGTTATTTTGTTCTCTAGTGGCTAATTTGATTATCTTTTTATGAATAAATCAGGTGCTGGATCTAACCCACAATGGAATGAGACTTTTCTTTTCACTGTTTCTGACAATGCTTCTGAGCTGAATCTAAAGATAATGGAAAAGGACAACTATAATAATGATGATAATATTGGAGAAGCAATGTGAGATGAATTCTTCTTAAACTTGCAAATTTGTCTTTGATTCATCTTTTTAGGTTGAATAAATCGTAATATCGACAAGTAAATCGACTTTGTAACTGTAATTTAAAAATACTTCCTCCAGAATGAAATATAAGCAAAAAAGTAGGTGAAAAGTAGACTAATTTTTTCGCCACATACGTCGACTTTGCAACTACCACTTTTGTTTATATTTCATTATGGAGAAAGTAAGTGTTAAAGTTGGATTCTCATTGAAAGTTGGTCTCTTAAACTAGTTTGTTTAAAAATTTACACCTAAAGGAGGGCACAAAGGAAAAATTTGCAATTTTTGCATACTAATTATTCATTTAGATATGATTTAAAGTGCGTCCTTTGAAGTTACATAGTCAAACTCTTACCACTGACATCTCGTTCAATTTTGACATACTATATATTCATTCATTTCTATCTCTAGAATTCCTTTAGAAGCAGTGTTTGAAGAGGGTAGCCTTCCAGAGAATTCTTACAATCTTGTGAAGGATGAAGAATATTGTGGAGAGATTAAGGTGGCTCTCACTTTTACACCTGAGGTAATAAGGCTATATAATTTCTTTTTCTATATTATTTTTCTTTATAGTTTATGCATATTTTTCTCCCTATTAAAATGTACTATCAATTATTGGTTGTAGAGAGATGATGATCAGAGTTACAATGAAGAGGAGGAGACTGGTGGATGGAAAGAATCAGCTAGTGACTTTTGA

Protein sequence

>MS.gene58248.t1

MPRGTLEVILISAKGLEDNDFLSSIDPYVILSYKAQEHKSKVQEGAGSNPQWNETFLFTVSDNASELNLKIMEKDNYNNDDNIGEAIIPLEAVFEEGSLPENSYNLVKDEEYCGEIKVALTFTPERDDDQSYNEEEETGGWKESASDF