Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63427.t1 | KEH33239.1 | 49 | 253 | 23 | 1 | 1 | 253 | 8 | 154 | 3.60E-51 | 211.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63427.t1 | Q9FKI4 | 41.4 | 99 | 49 | 2 | 6 | 95 | 2 | 100 | 1.5e-10 | 68.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63427.t1 | A0A072UTR7 | 49.0 | 253 | 23 | 1 | 1 | 253 | 8 | 154 | 2.6e-51 | 211.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene63427.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene63427 | MS.gene41916 | PPI |
| MS.gene63427 | MS.gene77827 | PPI |
| MS.gene63427 | MS.gene024463 | PPI |
| MS.gene63427 | MS.gene067362 | PPI |
| MS.gene63427 | MS.gene037362 | PPI |
| MS.gene63427 | MS.gene62426 | PPI |
| MS.gene63427 | MS.gene037324 | PPI |
| MS.gene63427 | MS.gene050629 | PPI |
| MS.gene63427 | MS.gene61498 | PPI |
| MS.gene63427 | MS.gene054186 | PPI |
| MS.gene63427 | MS.gene045843 | PPI |
| MS.gene63427 | MS.gene056257 | PPI |
| MS.gene63427 | MS.gene65650 | PPI |
| MS.gene63427 | MS.gene01518 | PPI |
| MS.gene63427 | MS.gene22814 | PPI |
| MS.gene63427 | MS.gene029211 | PPI |
| MS.gene036800 | MS.gene63427 | PPI |
| MS.gene63427 | MS.gene002540 | PPI |
| MS.gene63427 | MS.gene93131 | PPI |
| MS.gene63427 | MS.gene99744 | PPI |
| MS.gene63427 | MS.gene029242 | PPI |
| MS.gene59047 | MS.gene63427 | PPI |
| MS.gene63427 | MS.gene025927 | PPI |
| MS.gene63427 | MS.gene020996 | PPI |
| MS.gene63427 | MS.gene038837 | PPI |
| MS.gene63427 | MS.gene004214 | PPI |
| MS.gene059422 | MS.gene63427 | PPI |
| MS.gene63427 | MS.gene001986 | PPI |
| MS.gene63427 | MS.gene38111 | PPI |
| MS.gene63427 | MS.gene047400 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63427.t1 | MTR_3g030055 | 81.633 | 98 | 18 | 0 | 1 | 98 | 8 | 105 | 1.15e-52 | 168 |
| MS.gene63427.t1 | MTR_3g030055 | 67.647 | 68 | 22 | 0 | 186 | 253 | 87 | 154 | 4.50e-24 | 94.7 |
| MS.gene63427.t1 | MTR_3g030055 | 84.211 | 95 | 15 | 0 | 1 | 95 | 1 | 95 | 1.71e-51 | 164 |
| MS.gene63427.t1 | MTR_3g034200 | 42.991 | 107 | 48 | 4 | 6 | 102 | 2 | 105 | 1.44e-15 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63427.t1 | AT5G13780 | 42.000 | 100 | 47 | 3 | 6 | 95 | 2 | 100 | 9.55e-14 | 68.2 |
Find 62 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGAGTGGGTTGTGTTCTT+AGG | 0.147686 | 4.4:-60738984 | MS.gene63427:CDS |
| TTTGCAGGTGGTGAAATTAT+TGG | 0.274198 | 4.4:-60739003 | MS.gene63427:intron |
| GGTTAAGTCGGCGGCAAATA+TGG | 0.284105 | 4.4:+60739933 | None:intergenic |
| TTCCACAATCTGCCAATATT+CGG | 0.284634 | 4.4:+60742738 | None:intergenic |
| TCTTGGTTTGTTTGAAAAGA+TGG | 0.323368 | 4.4:-60735250 | MS.gene63427:CDS |
| CTCGTTACTCGCCCGAATAT+TGG | 0.336302 | 4.4:-60742750 | MS.gene63427:CDS |
| TCCACAATCTGCCAATATTC+GGG | 0.340184 | 4.4:+60742739 | None:intergenic |
| CTAGGATTATCTTCTATTCC+TGG | 0.344667 | 4.4:-60735680 | MS.gene63427:CDS |
| TGGAGTGGGTTGTGTTCTTA+GGG | 0.360382 | 4.4:-60738983 | MS.gene63427:CDS |
| GAGTTGCAGAGTTGCTCTTC+CGG | 0.364191 | 4.4:+60735271 | None:intergenic |
| TATTCCTGGTAGTGGTTCTT+CGG | 0.401419 | 4.4:-60735666 | MS.gene63427:intron |
| CTTCAAAATTGACTTCCATA+TGG | 0.405838 | 4.4:+60739226 | None:intergenic |
| TTAGGGTTCTGTACATATCT+AGG | 0.420390 | 4.4:-60738966 | MS.gene63427:intron |
| TGCATAAGAGTCGTAACATT+TGG | 0.424993 | 4.4:-60742804 | MS.gene63427:CDS |
| CATTTGGAGACGTATTAGAT+TGG | 0.435564 | 4.4:-60742788 | MS.gene63427:CDS |
| TTATCTTCTATTCCTGGTAG+TGG | 0.439635 | 4.4:-60735674 | MS.gene63427:CDS |
| CCATTGATTGTCGGTGGTGG+TGG | 0.446067 | 4.4:+60739883 | None:intergenic |
| CTTGGTTTGTTTGAAAAGAT+GGG | 0.446348 | 4.4:-60735249 | MS.gene63427:CDS |
| GTTGCTTACCTCCTCTCTAA+AGG | 0.454440 | 4.4:-60742675 | MS.gene63427:CDS |
| CTTCTCATCATCAAAATACT+TGG | 0.455146 | 4.4:+60735203 | None:intergenic |
| AAGAGCAACTCTGCAACTCT+TGG | 0.460789 | 4.4:-60735267 | MS.gene63427:CDS |
| GAGTGGCTCTCACAGTTAGA+TGG | 0.462257 | 4.4:+60735137 | None:intergenic |
| CCACCACCACCGACAATCAA+TGG | 0.489302 | 4.4:-60739883 | MS.gene63427:CDS |
| AAGACGTCTCAGTGCACGTC+CGG | 0.493597 | 4.4:-60735290 | MS.gene63427:CDS |
| TGCCGAAAACACCATGGAAA+AGG | 0.499281 | 4.4:-60742559 | MS.gene63427:intron |
| CCAGCATATTGTACACAGAA+TGG | 0.507865 | 4.4:+60738292 | None:intergenic |
| AATCTAAGTTTCACTAGGCT+AGG | 0.513674 | 4.4:-60735698 | MS.gene63427:CDS |
| TCTCTAAAGGTGAAGAGGAA+TGG | 0.513826 | 4.4:-60742662 | MS.gene63427:CDS |
| GCCCGAATATTGGCAGATTG+TGG | 0.515192 | 4.4:-60742740 | MS.gene63427:CDS |
| CAGCATATTGTACACAGAAT+GGG | 0.520756 | 4.4:+60738293 | None:intergenic |
| CCATTCTGTGTACAATATGC+TGG | 0.535184 | 4.4:-60738292 | MS.gene63427:intron |
| GAGTAGAAACCATTGATTGT+CGG | 0.538972 | 4.4:+60739874 | None:intergenic |
| CCTCTTCACCTTTAGAGAGG+AGG | 0.539853 | 4.4:+60742667 | None:intergenic |
| CCTCCTCTCTAAAGGTGAAG+AGG | 0.541904 | 4.4:-60742667 | MS.gene63427:CDS |
| AGGGAAAATGTCGTCAAAGA+AGG | 0.547752 | 4.4:-60742829 | None:intergenic |
| GGTGGATTCATCAGCACTGA+AGG | 0.547906 | 4.4:+60739901 | None:intergenic |
| GTCTTGAGGAAGATACTCAA+GGG | 0.554037 | 4.4:-60735171 | MS.gene63427:CDS |
| TTCACCGAAGAACCACTACC+AGG | 0.557386 | 4.4:+60735662 | None:intergenic |
| TGTCTTGAGGAAGATACTCA+AGG | 0.562517 | 4.4:-60735172 | MS.gene63427:CDS |
| CAGCACTGAAGGCGGAGGAT+CGG | 0.569513 | 4.4:+60739912 | None:intergenic |
| ATAGGTATGGTGAACAAAGA+GGG | 0.575631 | 4.4:+60742617 | None:intergenic |
| AAACCATTGATTGTCGGTGG+TGG | 0.576785 | 4.4:+60739880 | None:intergenic |
| GGTGGTGAAATTATTGGAGT+GGG | 0.585203 | 4.4:-60738997 | MS.gene63427:CDS |
| ATTCCTCTTCACCTTTAGAG+AGG | 0.588352 | 4.4:+60742664 | None:intergenic |
| TTCATCAGCACTGAAGGCGG+AGG | 0.588611 | 4.4:+60739907 | None:intergenic |
| TACCACATTTACGATAGGTA+TGG | 0.589360 | 4.4:+60742604 | None:intergenic |
| CACCATACCTATCGTAAATG+TGG | 0.595431 | 4.4:-60742606 | MS.gene63427:CDS |
| GATAGGTATGGTGAACAAAG+AGG | 0.607709 | 4.4:+60742616 | None:intergenic |
| TTACACACTTCACGAGGTGA+AGG | 0.607853 | 4.4:-60735226 | MS.gene63427:CDS |
| GTTACAATCTAAGTTTCACT+AGG | 0.610544 | 4.4:-60735703 | MS.gene63427:CDS |
| CACTGCTGCCGAAAACACCA+TGG | 0.615785 | 4.4:-60742565 | MS.gene63427:CDS |
| AGAAGGGCGCTATTGTCTTG+AGG | 0.624802 | 4.4:-60735185 | MS.gene63427:CDS |
| AGGTGGTGAAATTATTGGAG+TGG | 0.625774 | 4.4:-60738998 | MS.gene63427:CDS |
| CGGAGGATCGGTTAAGTCGG+CGG | 0.630252 | 4.4:+60739924 | None:intergenic |
| AGGCGGAGGATCGGTTAAGT+CGG | 0.636993 | 4.4:+60739921 | None:intergenic |
| TGGTTTCTACTCGAACTCAA+CGG | 0.645728 | 4.4:-60739863 | MS.gene63427:intron |
| TAGAAACCATTGATTGTCGG+TGG | 0.647279 | 4.4:+60739877 | None:intergenic |
| CACATCGCGGATGATTGCAG+TGG | 0.650393 | 4.4:-60742705 | MS.gene63427:CDS |
| GGATTCATCAGCACTGAAGG+CGG | 0.668779 | 4.4:+60739904 | None:intergenic |
| AGCGATACCACATTTACGAT+AGG | 0.672035 | 4.4:+60742599 | None:intergenic |
| TCTTGAGGAAGATACTCAAG+GGG | 0.688967 | 4.4:-60735170 | MS.gene63427:CDS |
| GATGGGTTACACACTTCACG+AGG | 0.713948 | 4.4:-60735232 | MS.gene63427:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 60735152 | 60742844 | 60735152 | ID=MS.gene63427 |
| chr4.4 | mRNA | 60735152 | 60742844 | 60735152 | ID=MS.gene63427.t1;Parent=MS.gene63427 |
| chr4.4 | exon | 60742560 | 60742844 | 60742560 | ID=MS.gene63427.t1.exon1;Parent=MS.gene63427.t1 |
| chr4.4 | CDS | 60742560 | 60742844 | 60742560 | ID=cds.MS.gene63427.t1;Parent=MS.gene63427.t1 |
| chr4.4 | exon | 60739864 | 60739959 | 60739864 | ID=MS.gene63427.t1.exon2;Parent=MS.gene63427.t1 |
| chr4.4 | CDS | 60739864 | 60739959 | 60739864 | ID=cds.MS.gene63427.t1;Parent=MS.gene63427.t1 |
| chr4.4 | exon | 60739209 | 60739261 | 60739209 | ID=MS.gene63427.t1.exon3;Parent=MS.gene63427.t1 |
| chr4.4 | CDS | 60739209 | 60739261 | 60739209 | ID=cds.MS.gene63427.t1;Parent=MS.gene63427.t1 |
| chr4.4 | exon | 60739081 | 60739103 | 60739081 | ID=MS.gene63427.t1.exon4;Parent=MS.gene63427.t1 |
| chr4.4 | CDS | 60739081 | 60739103 | 60739081 | ID=cds.MS.gene63427.t1;Parent=MS.gene63427.t1 |
| chr4.4 | exon | 60738967 | 60739018 | 60738967 | ID=MS.gene63427.t1.exon5;Parent=MS.gene63427.t1 |
| chr4.4 | CDS | 60738967 | 60739018 | 60738967 | ID=cds.MS.gene63427.t1;Parent=MS.gene63427.t1 |
| chr4.4 | exon | 60738293 | 60738315 | 60738293 | ID=MS.gene63427.t1.exon6;Parent=MS.gene63427.t1 |
| chr4.4 | CDS | 60738293 | 60738315 | 60738293 | ID=cds.MS.gene63427.t1;Parent=MS.gene63427.t1 |
| chr4.4 | exon | 60735667 | 60735728 | 60735667 | ID=MS.gene63427.t1.exon7;Parent=MS.gene63427.t1 |
| chr4.4 | CDS | 60735667 | 60735728 | 60735667 | ID=cds.MS.gene63427.t1;Parent=MS.gene63427.t1 |
| chr4.4 | exon | 60735152 | 60735322 | 60735152 | ID=MS.gene63427.t1.exon8;Parent=MS.gene63427.t1 |
| chr4.4 | CDS | 60735152 | 60735322 | 60735152 | ID=cds.MS.gene63427.t1;Parent=MS.gene63427.t1 |
| Gene Sequence |
| Protein sequence |
