AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65465

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65465.t1 MTR_1g042160 71.429 308 43 3 1 263 1 308 2.94e-151 424
MS.gene65465.t1 MTR_7g107200 35.714 378 128 6 1 265 1 376 3.09e-72 226
MS.gene65465.t1 MTR_7g107160 36.585 328 139 9 2 263 119 443 1.22e-53 180
MS.gene65465.t1 MTR_7g107160 47.619 105 55 0 69 173 1 105 3.17e-27 109
MS.gene65465.t1 MTR_3g079840 49.618 131 64 1 13 143 56 184 1.01e-40 150
MS.gene65465.t1 MTR_3g079840 49.618 131 64 1 13 143 57 185 1.01e-40 150
MS.gene65465.t1 MTR_5g086700 50.000 128 62 1 13 140 61 186 5.14e-40 148
MS.gene65465.t1 MTR_5g086700 50.000 128 62 1 13 140 61 186 5.60e-40 147
MS.gene65465.t1 MTR_1g041240 47.656 128 64 2 13 140 59 183 7.88e-35 133
MS.gene65465.t1 MTR_1g041240 47.656 128 64 2 13 140 58 182 7.96e-35 133
MS.gene65465.t1 MTR_1g041240 47.656 128 64 2 13 140 57 181 8.43e-35 132
MS.gene65465.t1 MTR_5g091450 43.651 126 70 1 13 138 33 157 2.36e-34 131
MS.gene65465.t1 MTR_7g107170 45.963 161 44 4 152 269 109 269 1.52e-30 115
MS.gene65465.t1 MTR_5g091180 42.063 126 72 1 13 138 46 170 2.98e-29 116
MS.gene65465.t1 MTR_8g011730 43.511 131 71 2 11 139 12 141 1.25e-26 106
MS.gene65465.t1 MTR_5g091550 36.220 127 79 1 12 138 18 142 7.00e-25 103
MS.gene65465.t1 MTR_5g091550 36.220 127 79 1 12 138 18 142 7.34e-25 103
MS.gene65465.t1 MTR_3g106700 31.655 139 86 2 13 142 406 544 8.13e-20 89.4
MS.gene65465.t1 MTR_3g106700 29.457 129 88 3 16 141 71 199 1.37e-14 73.9
MS.gene65465.t1 MTR_1g073790 31.757 148 95 4 2 148 51 193 7.21e-18 83.6
MS.gene65465.t1 MTR_1g044450 33.333 141 88 4 9 148 61 196 2.47e-17 82.0
MS.gene65465.t1 MTR_1g073800 30.405 148 99 4 2 148 38 182 3.67e-16 78.2
MS.gene65465.t1 MTR_8g006190 28.986 138 92 3 26 158 5 141 4.87e-15 74.7
MS.gene65465.t1 MTR_4g123750 28.889 135 88 3 14 140 20 154 1.59e-14 72.4
MS.gene65465.t1 MTR_4g123750 29.630 135 93 2 13 146 176 309 6.42e-12 65.1
MS.gene65465.t1 MTR_5g091530 35.632 87 56 0 54 140 1 87 1.41e-13 70.1
MS.gene65465.t1 MTR_8g006190 28.689 122 81 3 42 158 13 133 1.18e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65465.t1 AT5G06600 52.344 128 59 1 13 140 56 181 9.99e-40 147
MS.gene65465.t1 AT5G06600 52.344 128 59 1 13 140 56 181 1.25e-39 147
MS.gene65465.t1 AT5G06600 52.344 128 59 1 13 140 55 180 1.31e-39 147
MS.gene65465.t1 AT3G11910 52.344 128 59 1 13 140 54 179 7.02e-39 144
MS.gene65465.t1 AT3G11910 52.344 128 59 1 13 140 54 179 7.02e-39 144
MS.gene65465.t1 AT3G11910 52.344 128 59 1 13 140 55 180 7.16e-39 144
MS.gene65465.t1 AT3G11910 52.344 128 59 1 13 140 55 180 7.16e-39 144
MS.gene65465.t1 AT3G58250 40.152 132 79 0 12 143 9 140 4.14e-29 112
MS.gene65465.t1 AT3G58270 42.222 135 76 1 9 143 4 136 1.82e-28 111
MS.gene65465.t1 AT3G58270 42.222 135 76 1 9 143 4 136 1.82e-28 111
MS.gene65465.t1 AT3G58270 42.222 135 76 1 9 143 4 136 1.82e-28 111
MS.gene65465.t1 AT3G58340 30.000 160 105 2 12 166 7 164 2.21e-22 94.4
MS.gene65465.t1 AT3G58360 37.778 135 82 1 9 143 4 136 2.24e-22 94.4
MS.gene65465.t1 AT3G58340 34.091 132 85 1 12 143 7 136 3.04e-22 94.4
MS.gene65465.t1 AT3G58370 38.129 139 83 2 7 143 3 140 1.15e-20 89.7
MS.gene65465.t1 AT3G58350 35.338 133 83 2 12 142 7 138 6.26e-20 87.4
MS.gene65465.t1 AT3G58350 35.338 133 83 2 12 142 7 138 6.26e-20 87.4
MS.gene65465.t1 AT3G58210 33.588 131 87 0 13 143 8 138 6.76e-20 87.8
MS.gene65465.t1 AT2G05410 35.252 139 87 2 5 143 2 137 1.19e-19 86.7
MS.gene65465.t1 AT3G58410 32.331 133 85 2 9 141 4 131 1.17e-18 84.3
MS.gene65465.t1 AT2G25320 30.137 146 92 3 13 149 404 548 1.03e-17 83.2
MS.gene65465.t1 AT2G25320 30.233 129 87 3 16 141 75 203 2.16e-15 76.6
MS.gene65465.t1 AT2G25320 30.137 146 92 3 13 149 405 549 1.07e-17 83.2
MS.gene65465.t1 AT2G25320 30.233 129 87 3 16 141 76 204 2.18e-15 76.3
MS.gene65465.t1 AT3G27040 31.818 132 89 1 12 143 8 138 1.19e-17 80.9
MS.gene65465.t1 AT3G58260 31.387 137 92 2 9 143 4 140 1.48e-17 81.3
MS.gene65465.t1 AT3G58290 30.000 140 88 3 12 142 10 148 1.58e-17 80.5
MS.gene65465.t1 AT2G25320 30.137 146 92 3 13 149 405 549 1.75e-17 82.8
MS.gene65465.t1 AT2G25320 30.233 129 87 3 16 141 76 204 2.22e-15 76.3
MS.gene65465.t1 AT3G17380 29.605 152 99 3 17 163 25 173 3.65e-17 80.1
MS.gene65465.t1 AT3G17380 27.407 135 96 2 13 146 175 308 2.40e-12 66.2
MS.gene65465.t1 AT2G25330 32.374 139 85 2 13 142 359 497 4.13e-17 81.3
MS.gene65465.t1 AT2G25330 31.008 129 86 3 16 141 50 178 4.72e-15 75.1
MS.gene65465.t1 AT3G44800 33.083 133 88 1 11 143 3 134 1.36e-16 79.0
MS.gene65465.t1 AT2G01790 32.558 129 86 1 16 143 11 139 1.80e-16 77.4
MS.gene65465.t1 AT5G52330 33.600 125 78 3 9 133 16 135 9.81e-16 76.6
MS.gene65465.t1 AT5G52330 33.600 125 78 3 9 133 16 135 9.81e-16 76.6
MS.gene65465.t1 AT5G52330 33.600 125 78 3 9 133 16 135 9.81e-16 76.6
MS.gene65465.t1 AT3G44790 33.835 133 83 2 11 143 3 130 3.21e-15 74.7
MS.gene65465.t1 AT5G43560 32.624 141 89 4 9 148 66 201 4.11e-15 75.5
MS.gene65465.t1 AT5G43560 32.624 141 89 4 9 148 66 201 4.11e-15 75.5
MS.gene65465.t1 AT3G58200 32.759 116 75 2 13 127 8 121 1.10e-14 73.2
MS.gene65465.t1 AT3G58440 33.077 130 83 4 12 141 9 134 1.66e-14 73.6
MS.gene65465.t1 AT1G04300 33.600 125 78 3 9 133 65 184 2.64e-14 73.2
MS.gene65465.t1 AT2G05420 30.147 136 88 2 14 143 10 144 7.85e-14 70.5
MS.gene65465.t1 AT3G58410 30.000 130 72 3 9 138 25 135 8.43e-14 70.5
MS.gene65465.t1 AT2G42480 30.282 142 90 5 14 152 8 143 9.58e-13 67.8
MS.gene65465.t1 AT4G09770 28.462 130 87 4 21 149 24 148 1.07e-12 66.6
MS.gene65465.t1 AT4G09770 28.462 130 87 4 21 149 24 148 1.26e-12 67.0
MS.gene65465.t1 AT4G09770 28.462 130 87 4 21 149 24 148 1.26e-12 67.0
MS.gene65465.t1 AT1G31390 29.412 119 83 1 12 129 7 125 1.42e-12 66.6
MS.gene65465.t1 AT4G09770 28.462 130 87 4 21 149 50 174 1.51e-12 66.6
MS.gene65465.t1 AT1G04300 32.331 133 77 4 9 133 65 192 1.65e-12 67.8
MS.gene65465.t1 AT4G09770 28.462 130 87 4 21 149 50 174 1.75e-12 66.6
MS.gene65465.t1 AT5G26260 29.078 141 91 4 17 149 63 202 1.91e-12 67.0
MS.gene65465.t1 AT4G09780 34.677 124 77 4 13 134 214 335 3.63e-12 66.2
MS.gene65465.t1 AT1G31400 28.571 133 91 2 12 143 7 136 1.75e-11 63.5
MS.gene65465.t1 AT5G26320 29.839 124 81 3 31 149 81 203 2.35e-11 63.5
MS.gene65465.t1 AT2G32870 31.852 135 85 3 13 141 277 410 2.63e-11 63.5
MS.gene65465.t1 AT2G32880 31.655 139 81 5 13 141 178 312 6.09e-11 62.4
MS.gene65465.t1 AT2G42460 33.088 136 87 3 8 143 4 135 6.43e-11 62.0
MS.gene65465.t1 AT3G58240 27.586 174 121 3 13 183 8 179 8.04e-11 62.0
MS.gene65465.t1 AT4G01390 32.593 135 84 5 12 143 166 296 8.28e-11 61.6
MS.gene65465.t1 AT3G58220 27.481 131 92 3 14 143 13 141 8.62e-11 62.0
MS.gene65465.t1 AT3G58220 27.481 131 92 3 14 143 13 141 8.87e-11 61.2
MS.gene65465.t1 AT3G58220 27.481 131 92 3 14 143 13 141 9.24e-11 61.6

Find 61 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAAAAGAGTTTCAATATT+TGG 0.072812 1.1:-26061226 None:intergenic
GTGAATGATACTTGTATTAT+TGG 0.182812 1.1:+26061064 MS.gene65465:CDS
CAACCAGTTACATATATATT+TGG 0.218251 1.1:+26060538 MS.gene65465:CDS
GTAAAAGAGTTTCAATATTT+GGG 0.278091 1.1:-26061225 None:intergenic
GAAGGAGAGCATGGTAGTTC+TGG 0.285409 1.1:+26061504 MS.gene65465:CDS
GACCTTCAGGCTTTGCTGTT+GGG 0.323931 1.1:-26061198 None:intergenic
ACTGCACTATTCTTCCCCTT+TGG 0.324707 1.1:-26060515 None:intergenic
CTGCACTATTCTTCCCCTTT+GGG 0.326451 1.1:-26060514 None:intergenic
AGTGAGGATGACTGGGGATT+TGG 0.328601 1.1:+26060992 MS.gene65465:CDS
TCCGAGCCTTTCGTCCTTAG+TGG 0.329886 1.1:+26060359 MS.gene65465:CDS
ATATTTGGGCCTCGACCTTC+AGG 0.334872 1.1:-26061211 None:intergenic
GTAGATTTAGATTCTGAATT+GGG 0.350592 1.1:+26061622 MS.gene65465:CDS
GGCTTGCTCGTTCATGTCTC+TGG 0.355614 1.1:-26061350 None:intergenic
AGTAGATTTAGATTCTGAAT+TGG 0.356087 1.1:+26061621 MS.gene65465:CDS
CGACCTTCAGGCTTTGCTGT+TGG 0.374349 1.1:-26061199 None:intergenic
CCTCCAAATATATATGTAAC+TGG 0.386489 1.1:-26060541 None:intergenic
GAGTCGGTGTTTGCTGCATT+AGG 0.394888 1.1:+26061295 MS.gene65465:CDS
AGAGACTTGTTGAAATCAAA+AGG 0.412312 1.1:+26061589 MS.gene65465:CDS
GGTAGTTCTGGAGCTCGAAT+TGG 0.424459 1.1:+26061516 MS.gene65465:CDS
AAGATATGTGGAGAAAGCTT+TGG 0.447649 1.1:+26061468 MS.gene65465:CDS
TTAGCTGAGCTTAGTGATCC+TGG 0.453456 1.1:+26061028 MS.gene65465:CDS
TCAGCGCAAGTGAGGATGAC+TGG 0.460636 1.1:+26060984 MS.gene65465:CDS
AGGATTCTTCTCTTCCCAAA+GGG 0.475030 1.1:+26060500 MS.gene65465:CDS
GCAAACACCGACTCTGCGTC+AGG 0.489005 1.1:-26061286 None:intergenic
TTAGATTCTGAATTGGGATG+TGG 0.494944 1.1:+26061628 MS.gene65465:CDS
GACAACAGTACAATCACAAA+AGG 0.495774 1.1:+26060641 MS.gene65465:CDS
TAGATTCTGAATTGGGATGT+GGG 0.496207 1.1:+26061629 MS.gene65465:CDS
TTTGGAGGCTGAAAAGTTGA+AGG 0.501303 1.1:+26061486 MS.gene65465:CDS
GAATTGGGATGTGGGAGTTG+TGG 0.508997 1.1:+26061637 MS.gene65465:CDS
CCAGTTACATATATATTTGG+AGG 0.512397 1.1:+26060541 MS.gene65465:CDS
CAGCGCAAGTGAGGATGACT+GGG 0.513280 1.1:+26060985 MS.gene65465:CDS
ATATGTGGAGAAAGCTTTGG+AGG 0.523862 1.1:+26061471 MS.gene65465:CDS
CCTCACTTGCGCTGAACTCG+TGG 0.540550 1.1:-26060976 None:intergenic
GCTCGAATTGGAAAGGCTAA+AGG 0.542142 1.1:+26061528 MS.gene65465:CDS
ACAGCAAAGCCTGAAGGTCG+AGG 0.543195 1.1:+26061202 MS.gene65465:CDS
TGAGCTTAGTGATCCTGGAA+GGG 0.551733 1.1:+26061033 MS.gene65465:CDS
GAGCTTAGTGATCCTGGAAG+GGG 0.560810 1.1:+26061034 MS.gene65465:CDS
CTGAGCTTAGTGATCCTGGA+AGG 0.562085 1.1:+26061032 MS.gene65465:CDS
AGCTTAGTGATCCTGGAAGG+GGG 0.579102 1.1:+26061035 MS.gene65465:CDS
CTTACCATGGATATCCACTA+AGG 0.579500 1.1:-26060373 None:intergenic
AAAGACTGCTAATATGTCTG+AGG 0.579958 1.1:+26060568 MS.gene65465:CDS
TGAAAAGTTGAAGGAGAGCA+TGG 0.580493 1.1:+26061495 MS.gene65465:CDS
TGATACTTGTATTATTGGTG+TGG 0.585879 1.1:+26061069 MS.gene65465:CDS
TCCACTAAGGACGAAAGGCT+CGG 0.589489 1.1:-26060360 None:intergenic
CTGCTAATATGTCTGAGGGA+TGG 0.590956 1.1:+26060573 MS.gene65465:CDS
GAGGATTCTTCTCTTCCCAA+AGG 0.594512 1.1:+26060499 MS.gene65465:CDS
TTCTGGAGCTCGAATTGGAA+AGG 0.594903 1.1:+26061521 MS.gene65465:CDS
CCACGAGTTCAGCGCAAGTG+AGG 0.596224 1.1:+26060976 MS.gene65465:CDS
TGCTGTATTCAATCAAGTCG+AGG 0.599719 1.1:+26060619 MS.gene65465:CDS
TCGTCCTTAGTGGATATCCA+TGG 0.600369 1.1:+26060369 MS.gene65465:CDS
AAGACTGCTAATATGTCTGA+GGG 0.604084 1.1:+26060569 MS.gene65465:CDS
CTCCCAACAGCAAAGCCTGA+AGG 0.610004 1.1:+26061196 MS.gene65465:CDS
TAAAGATCCTGACGCAGAGT+CGG 0.611168 1.1:+26061279 MS.gene65465:CDS
TAACTTATCAACATTCAAGT+GGG 0.611555 1.1:-26060333 None:intergenic
GGATATCCACTAAGGACGAA+AGG 0.618904 1.1:-26060365 None:intergenic
CTCGAGTTTACAGAAATGGA+GGG 0.619993 1.1:+26060254 None:intergenic
ATAACTTATCAACATTCAAG+TGG 0.634087 1.1:-26060334 None:intergenic
GGATTCTTCTCTTCCCAAAG+GGG 0.641187 1.1:+26060501 MS.gene65465:CDS
GGAAAGGCTAAAGGTCAAGT+CGG 0.643411 1.1:+26061537 MS.gene65465:CDS
AGATATTTGAGAAGTTCACA+TGG 0.666468 1.1:+26060294 MS.gene65465:CDS
AGCGCAAGTGAGGATGACTG+GGG 0.764616 1.1:+26060986 MS.gene65465:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAAATATGATATTACT+TGG + chr1.1:26061410-26061429 MS.gene65465:intron 10.0%
!! GTAAAAGAGTTTCAATATTT+GGG - chr1.1:26061228-26061247 None:intergenic 20.0%
!! TGTAAAAGAGTTTCAATATT+TGG - chr1.1:26061229-26061248 None:intergenic 20.0%
!!! ATTTGTGTTTTGATCTTATT+TGG + chr1.1:26060465-26060484 MS.gene65465:intron 20.0%
! AGTAGATTTAGATTCTGAAT+TGG + chr1.1:26061621-26061640 MS.gene65465:CDS 25.0%
! AGTCAAATTACTTGTGTAAA+AGG - chr1.1:26060898-26060917 None:intergenic 25.0%
! ATAACTTATCAACATTCAAG+TGG - chr1.1:26060337-26060356 None:intergenic 25.0%
! ATAATCTATTAATCTACCAG+TGG - chr1.1:26060421-26060440 None:intergenic 25.0%
! CAACCAGTTACATATATATT+TGG + chr1.1:26060538-26060557 MS.gene65465:CDS 25.0%
! GTAGATTTAGATTCTGAATT+GGG + chr1.1:26061622-26061641 MS.gene65465:CDS 25.0%
! GTCAAATTACTTGTGTAAAA+GGG - chr1.1:26060897-26060916 None:intergenic 25.0%
! GTGAATGATACTTGTATTAT+TGG + chr1.1:26061064-26061083 MS.gene65465:CDS 25.0%
! GTGTATGTTTGATATCATTA+TGG + chr1.1:26060703-26060722 MS.gene65465:intron 25.0%
! TAACTTATCAACATTCAAGT+GGG - chr1.1:26060336-26060355 None:intergenic 25.0%
! TTCATTCAAATAACTTACCA+TGG - chr1.1:26060389-26060408 None:intergenic 25.0%
! TTTAGGGATAAAAAGATATG+TGG + chr1.1:26061456-26061475 MS.gene65465:intron 25.0%
!!! ATCTTATTTGGTTTTGTTGT+AGG + chr1.1:26060477-26060496 MS.gene65465:intron 25.0%
!!! TAAGGATCTTCAAGTTTTAT+GGG + chr1.1:26061375-26061394 MS.gene65465:intron 25.0%
AGAGATATAGAGAGATTATG+TGG + chr1.1:26060830-26060849 MS.gene65465:intron 30.0%
AGATATTTGAGAAGTTCACA+TGG + chr1.1:26060294-26060313 MS.gene65465:CDS 30.0%
CCAGTTACATATATATTTGG+AGG + chr1.1:26060541-26060560 MS.gene65465:CDS 30.0%
CCTCCAAATATATATGTAAC+TGG - chr1.1:26060544-26060563 None:intergenic 30.0%
TAAAACTTGAAGATCCTTAC+AGG - chr1.1:26061374-26061393 None:intergenic 30.0%
TGATACTTGTATTATTGGTG+TGG + chr1.1:26061069-26061088 MS.gene65465:CDS 30.0%
TGCATGTTCATTATGCTTTA+GGG + chr1.1:26061440-26061459 MS.gene65465:intron 30.0%
TTGCATGTTCATTATGCTTT+AGG + chr1.1:26061439-26061458 MS.gene65465:intron 30.0%
! CACTTAATCATTTTGCAAGA+CGG - chr1.1:26060686-26060705 None:intergenic 30.0%
!! AGAGACTTGTTGAAATCAAA+AGG + chr1.1:26061589-26061608 MS.gene65465:CDS 30.0%
!! GTAAGGATCTTCAAGTTTTA+TGG + chr1.1:26061374-26061393 MS.gene65465:intron 30.0%
!!! TTATTTGGTTTTGTTGTAGG+AGG + chr1.1:26060480-26060499 MS.gene65465:intron 30.0%
AAAGACTGCTAATATGTCTG+AGG + chr1.1:26060568-26060587 MS.gene65465:CDS 35.0%
AAGACTGCTAATATGTCTGA+GGG + chr1.1:26060569-26060588 MS.gene65465:CDS 35.0%
AAGATATGTGGAGAAAGCTT+TGG + chr1.1:26061468-26061487 MS.gene65465:CDS 35.0%
AGTTTGACTACCAAACGTAA+GGG - chr1.1:26061158-26061177 None:intergenic 35.0%
GAATGAATCACAATTTCCAC+TGG + chr1.1:26060402-26060421 MS.gene65465:intron 35.0%
GACAACAGTACAATCACAAA+AGG + chr1.1:26060641-26060660 MS.gene65465:CDS 35.0%
TAGATTCTGAATTGGGATGT+GGG + chr1.1:26061629-26061648 MS.gene65465:CDS 35.0%
TCAAACTGGTTAAATGGAAG+TGG + chr1.1:26061171-26061190 MS.gene65465:intron 35.0%
TGGTAGTCAAACTGGTTAAA+TGG + chr1.1:26061165-26061184 MS.gene65465:intron 35.0%
TTAGATTCTGAATTGGGATG+TGG + chr1.1:26061628-26061647 MS.gene65465:CDS 35.0%
! TTAATCATTTTGCAAGACGG+AGG - chr1.1:26060683-26060702 None:intergenic 35.0%
AGGATTCTTCTCTTCCCAAA+GGG + chr1.1:26060500-26060519 MS.gene65465:CDS 40.0%
CAGTTTGACTACCAAACGTA+AGG - chr1.1:26061159-26061178 None:intergenic 40.0%
CTTACCATGGATATCCACTA+AGG - chr1.1:26060376-26060395 None:intergenic 40.0%
TACCAAACGTAAGGGATGAA+GGG - chr1.1:26061150-26061169 None:intergenic 40.0%
TAGAATCATGGTGATCCACT+AGG + chr1.1:26060732-26060751 MS.gene65465:intron 40.0%
TAGCTTCTGCAAAATCCTAG+TGG - chr1.1:26060750-26060769 None:intergenic 40.0%
TGAAAAGTTGAAGGAGAGCA+TGG + chr1.1:26061495-26061514 MS.gene65465:CDS 40.0%
TGCTGTATTCAATCAAGTCG+AGG + chr1.1:26060619-26060638 MS.gene65465:CDS 40.0%
TTATGGCTACGCTAGAATCA+TGG + chr1.1:26060720-26060739 MS.gene65465:intron 40.0%
TTTGGAGGCTGAAAAGTTGA+AGG + chr1.1:26061486-26061505 MS.gene65465:CDS 40.0%
! ATATGTGGAGAAAGCTTTGG+AGG + chr1.1:26061471-26061490 MS.gene65465:CDS 40.0%
! CTTACGTTTGGTAGTCAAAC+TGG + chr1.1:26061157-26061176 MS.gene65465:intron 40.0%
!! GGATCTTCAAGTTTTATGGG+AGG + chr1.1:26061378-26061397 MS.gene65465:intron 40.0%
!!! CTCTGGCTTTTCTAGTCTTA+AGG - chr1.1:26061336-26061355 None:intergenic 40.0%
ACTGCACTATTCTTCCCCTT+TGG - chr1.1:26060518-26060537 None:intergenic 45.0%
ATCCCTTCATCCCTTACGTT+TGG + chr1.1:26061145-26061164 MS.gene65465:intron 45.0%
CTACCAAACGTAAGGGATGA+AGG - chr1.1:26061151-26061170 None:intergenic 45.0%
CTGCACTATTCTTCCCCTTT+GGG - chr1.1:26060517-26060536 None:intergenic 45.0%
CTGCTAATATGTCTGAGGGA+TGG + chr1.1:26060573-26060592 MS.gene65465:CDS 45.0%
GAGGATTCTTCTCTTCCCAA+AGG + chr1.1:26060499-26060518 MS.gene65465:CDS 45.0%
GCTCGAATTGGAAAGGCTAA+AGG + chr1.1:26061528-26061547 MS.gene65465:CDS 45.0%
GGAAAGGCTAAAGGTCAAGT+CGG + chr1.1:26061537-26061556 MS.gene65465:CDS 45.0%
GGATATCCACTAAGGACGAA+AGG - chr1.1:26060368-26060387 None:intergenic 45.0%
GGATTCTTCTCTTCCCAAAG+GGG + chr1.1:26060501-26060520 MS.gene65465:CDS 45.0%
TAAAGATCCTGACGCAGAGT+CGG + chr1.1:26061279-26061298 MS.gene65465:CDS 45.0%
TGAGCTTAGTGATCCTGGAA+GGG + chr1.1:26061033-26061052 MS.gene65465:CDS 45.0%
TTAGCTGAGCTTAGTGATCC+TGG + chr1.1:26061028-26061047 MS.gene65465:CDS 45.0%
TTCACAATAAACCCCCTTCC+AGG - chr1.1:26061049-26061068 None:intergenic 45.0%
! ATTTTGCAAGACGGAGGAAG+AGG - chr1.1:26060677-26060696 None:intergenic 45.0%
! TCGTCCTTAGTGGATATCCA+TGG + chr1.1:26060369-26060388 MS.gene65465:CDS 45.0%
!! TTCTGGAGCTCGAATTGGAA+AGG + chr1.1:26061521-26061540 MS.gene65465:CDS 45.0%
AGCTTAGTGATCCTGGAAGG+GGG + chr1.1:26061035-26061054 MS.gene65465:CDS 50.0%
AGTGAGGATGACTGGGGATT+TGG + chr1.1:26060992-26061011 MS.gene65465:CDS 50.0%
ATATTTGGGCCTCGACCTTC+AGG - chr1.1:26061214-26061233 None:intergenic 50.0%
CATGAACGAGCAAGCCTGTA+AGG + chr1.1:26061357-26061376 MS.gene65465:intron 50.0%
CTGAGCTTAGTGATCCTGGA+AGG + chr1.1:26061032-26061051 MS.gene65465:CDS 50.0%
GAATTGGGATGTGGGAGTTG+TGG + chr1.1:26061637-26061656 MS.gene65465:CDS 50.0%
GAGCTTAGTGATCCTGGAAG+GGG + chr1.1:26061034-26061053 MS.gene65465:CDS 50.0%
TCCACTAAGGACGAAAGGCT+CGG - chr1.1:26060363-26060382 None:intergenic 50.0%
! GAAGGAGAGCATGGTAGTTC+TGG + chr1.1:26061504-26061523 MS.gene65465:CDS 50.0%
! GAGTCGGTGTTTGCTGCATT+AGG + chr1.1:26061295-26061314 MS.gene65465:CDS 50.0%
!! GACCTTCAGGCTTTGCTGTT+GGG - chr1.1:26061201-26061220 None:intergenic 50.0%
!! GGTAGTTCTGGAGCTCGAAT+TGG + chr1.1:26061516-26061535 MS.gene65465:CDS 50.0%
ACAGCAAAGCCTGAAGGTCG+AGG + chr1.1:26061202-26061221 MS.gene65465:CDS 55.0%
AGCGCAAGTGAGGATGACTG+GGG + chr1.1:26060986-26061005 MS.gene65465:CDS 55.0%
CAGCGCAAGTGAGGATGACT+GGG + chr1.1:26060985-26061004 MS.gene65465:CDS 55.0%
CTCCCAACAGCAAAGCCTGA+AGG + chr1.1:26061196-26061215 MS.gene65465:CDS 55.0%
TCAGCGCAAGTGAGGATGAC+TGG + chr1.1:26060984-26061003 MS.gene65465:CDS 55.0%
TCCGAGCCTTTCGTCCTTAG+TGG + chr1.1:26060359-26060378 MS.gene65465:CDS 55.0%
! CGACCTTCAGGCTTTGCTGT+TGG - chr1.1:26061202-26061221 None:intergenic 55.0%
! GGCTTGCTCGTTCATGTCTC+TGG - chr1.1:26061353-26061372 None:intergenic 55.0%
CCACGAGTTCAGCGCAAGTG+AGG + chr1.1:26060976-26060995 MS.gene65465:CDS 60.0%
CCTCACTTGCGCTGAACTCG+TGG - chr1.1:26060979-26060998 None:intergenic 60.0%
!! GCAAACACCGACTCTGCGTC+AGG - chr1.1:26061289-26061308 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 26060269 26061666 26060269 ID=MS.gene65465
chr1.1 mRNA 26060269 26061666 26060269 ID=MS.gene65465.t1;Parent=MS.gene65465
chr1.1 exon 26060269 26060390 26060269 ID=MS.gene65465.t1.exon1;Parent=MS.gene65465.t1
chr1.1 CDS 26060269 26060390 26060269 ID=cds.MS.gene65465.t1;Parent=MS.gene65465.t1
chr1.1 exon 26060499 26060662 26060499 ID=MS.gene65465.t1.exon2;Parent=MS.gene65465.t1
chr1.1 CDS 26060499 26060662 26060499 ID=cds.MS.gene65465.t1;Parent=MS.gene65465.t1
chr1.1 exon 26060969 26061103 26060969 ID=MS.gene65465.t1.exon3;Parent=MS.gene65465.t1
chr1.1 CDS 26060969 26061103 26060969 ID=cds.MS.gene65465.t1;Parent=MS.gene65465.t1
chr1.1 exon 26061191 26061373 26061191 ID=MS.gene65465.t1.exon4;Parent=MS.gene65465.t1
chr1.1 CDS 26061191 26061373 26061191 ID=cds.MS.gene65465.t1;Parent=MS.gene65465.t1
chr1.1 exon 26061461 26061666 26061461 ID=MS.gene65465.t1.exon5;Parent=MS.gene65465.t1
chr1.1 CDS 26061461 26061666 26061461 ID=cds.MS.gene65465.t1;Parent=MS.gene65465.t1
Gene Sequence

>MS.gene65465

ATGGAGGGTCAAGAAACTAGTGTTGAGATATTTGAGAAGTTCACATGGAAGATTGAAAATTTTTCCCACTTGAATGTTGATAAGTTATACTCCGAGCCTTTCGTCCTTAGTGGATATCCATGGTAAGTTATTTGAATGAATCACAATTTCCACTGGTAGATTAATAGATTATACACTCTTTGACATGATTTATTACATTTGTGTTTTGATCTTATTTGGTTTTGTTGTAGGAGGATTCTTCTCTTCCCAAAGGGGAAGAATAGTGCAGTCAACCAGTTACATATATATTTGGAGGCTATAAAGACTGCTAATATGTCTGAGGGATGGAAGAGAGATGCGAATTTTAAGTTTGCTGTATTCAATCAAGTCGAGGACAACAGTACAATCACAAAAGGTTTTTTCTCTCCTCTTCCTCCGTCTTGCAAAATGATTAAGTGTATGTTTGATATCATTATGGCTACGCTAGAATCATGGTGATCCACTAGGATTTTGCAGAAGCTAAAAAGTGAAAAAGAGACTGTGTGATATACATATGATAGTTTTATGATGTGATATAGAAGCAGAGATATAGAGAGATTATGTGGTATTTTTTTAATATATGTTCATAATATGATGCTTGCATTTTCCCTTTTACACAAGTAATTTGACTCAAGTAAGAAAAAAATAAACTAAATTTTACTCTTATGCTTTATCTTGACAGAAACTAGCCACGAGTTCAGCGCAAGTGAGGATGACTGGGGATTTGGTTCTTTCATGACATTAGCTGAGCTTAGTGATCCTGGAAGGGGGTTTATTGTGAATGATACTTGTATTATTGGTGTGGAAATTTTTGTTCGTAAGTCAACTCATGAGAAACGAATAAATCAAATCGTTAACATCCCTTCATCCCTTACGTTTGGTAGTCAAACTGGTTAAATGGAAGTGGAGCTCCCAACAGCAAAGCCTGAAGGTCGAGGCCCAAATATTGAAACTCTTTTACAAGTTTCTTCACTAGTTTCTACTGAAGCTACTAAAGATCCTGACGCAGAGTCGGTGTTTGCTGCATTAGGTAGAGTTATGTATTTCCTTAAGACTAGAAAAGCCAGAGACATGAACGAGCAAGCCTGTAAGGATCTTCAAGTTTTATGGGAGGAACTTGCAAAATTTAAATATGATATTACTTGGCTTGAATTGCATGTTCATTATGCTTTAGGGATAAAAAGATATGTGGAGAAAGCTTTGGAGGCTGAAAAGTTGAAGGAGAGCATGGTAGTTCTGGAGCTCGAATTGGAAAGGCTAAAGGTCAAGTCGGTTGCAACTAAGATGAATCTCGACATAGAAAGAGACTTGTTGAAATCAAAAGGTTTCAAAGAAGTAGATTTAGATTCTGAATTGGGATGTGGGAGTTGTGGACCATAG
Protein sequence

>MS.gene65465.t1

MEGQETSVEIFEKFTWKIENFSHLNVDKLYSEPFVLSGYPWRILLFPKGKNSAVNQLHIYLEAIKTANMSEGWKRDANFKFAVFNQVEDNSTITKETSHEFSASEDDWGFGSFMTLAELSDPGRGFIVNDTCIIGVEIFVLELPTAKPEGRGPNIETLLQVSSLVSTEATKDPDAESVFAALGRVMYFLKTRKARDMNEQAWIKRYVEKALEAEKLKESMVVLELELERLKVKSVATKMNLDIERDLLKSKGFKEVDLDSELGCGSCGP