Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66463.t1 | XP_003589063.1 | 91.4 | 292 | 17 | 2 | 1 | 291 | 1 | 285 | 1.30E-113 | 419.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66463.t1 | G7ICB3 | 91.4 | 292 | 17 | 2 | 1 | 291 | 1 | 285 | 9.4e-114 | 419.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049259 | MS.gene66463 | 0.835734 | 1.41E-56 | -1.69E-46 |
| MS.gene04986 | MS.gene66463 | 0.808424 | 3.12E-50 | -1.69E-46 |
| MS.gene050173 | MS.gene66463 | 0.828288 | 9.77E-55 | -1.69E-46 |
| MS.gene050216 | MS.gene66463 | -0.814871 | 1.24E-51 | -1.69E-46 |
| MS.gene050381 | MS.gene66463 | 0.813017 | 3.17E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene66463 | -0.8061 | 9.69E-50 | -1.69E-46 |
| MS.gene050871 | MS.gene66463 | -0.808389 | 3.17E-50 | -1.69E-46 |
| MS.gene051044 | MS.gene66463 | 0.824777 | 6.72E-54 | -1.69E-46 |
| MS.gene051127 | MS.gene66463 | 0.840323 | 9.32E-58 | -1.69E-46 |
| MS.gene051138 | MS.gene66463 | 0.838406 | 2.93E-57 | -1.69E-46 |
| MS.gene051863 | MS.gene66463 | 0.813713 | 2.23E-51 | -1.69E-46 |
| MS.gene052566 | MS.gene66463 | 0.81164 | 6.33E-51 | -1.69E-46 |
| MS.gene053069 | MS.gene66463 | 0.8194 | 1.18E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene66463 | 0.809104 | 2.23E-50 | -1.69E-46 |
| MS.gene053488 | MS.gene66463 | 0.810689 | 1.02E-50 | -1.69E-46 |
| MS.gene053561 | MS.gene66463 | 0.850045 | 2.19E-60 | -1.69E-46 |
| MS.gene054056 | MS.gene66463 | 0.858562 | 7.56E-63 | -1.69E-46 |
| MS.gene055124 | MS.gene66463 | 0.8225 | 2.29E-53 | -1.69E-46 |
| MS.gene055154 | MS.gene66463 | 0.812821 | 3.50E-51 | -1.69E-46 |
| MS.gene055776 | MS.gene66463 | -0.812894 | 3.37E-51 | -1.69E-46 |
| MS.gene055942 | MS.gene66463 | 0.862803 | 3.91E-64 | -1.69E-46 |
| MS.gene056188 | MS.gene66463 | 0.808874 | 2.50E-50 | -1.69E-46 |
| MS.gene058093 | MS.gene66463 | 0.806598 | 7.61E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene66463 | 0.843354 | 1.48E-58 | -1.69E-46 |
| MS.gene058109 | MS.gene66463 | 0.818592 | 1.81E-52 | -1.69E-46 |
| MS.gene058117 | MS.gene66463 | 0.806713 | 7.20E-50 | -1.69E-46 |
| MS.gene058171 | MS.gene66463 | 0.847651 | 1.01E-59 | -1.69E-46 |
| MS.gene058173 | MS.gene66463 | 0.831395 | 1.71E-55 | -1.69E-46 |
| MS.gene058175 | MS.gene66463 | 0.849341 | 3.44E-60 | -1.69E-46 |
| MS.gene058268 | MS.gene66463 | 0.804686 | 1.92E-49 | -1.69E-46 |
| MS.gene058493 | MS.gene66463 | 0.803814 | 2.91E-49 | -1.69E-46 |
| MS.gene058628 | MS.gene66463 | 0.824385 | 8.30E-54 | -1.69E-46 |
| MS.gene058629 | MS.gene66463 | 0.814637 | 1.39E-51 | -1.69E-46 |
| MS.gene058630 | MS.gene66463 | 0.8143 | 1.65E-51 | -1.69E-46 |
| MS.gene05884 | MS.gene66463 | 0.817041 | 4.05E-52 | -1.69E-46 |
| MS.gene059045 | MS.gene66463 | 0.840823 | 6.89E-58 | -1.69E-46 |
| MS.gene059690 | MS.gene66463 | 0.845205 | 4.70E-59 | -1.69E-46 |
| MS.gene059691 | MS.gene66463 | 0.806644 | 7.44E-50 | -1.69E-46 |
| MS.gene059694 | MS.gene66463 | 0.835894 | 1.29E-56 | -1.69E-46 |
| MS.gene059697 | MS.gene66463 | 0.82618 | 3.12E-54 | -1.69E-46 |
| MS.gene059865 | MS.gene66463 | -0.817829 | 2.69E-52 | -1.69E-46 |
| MS.gene059943 | MS.gene66463 | 0.801486 | 8.80E-49 | -1.69E-46 |
| MS.gene06012 | MS.gene66463 | 0.821326 | 4.28E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66463.t1 | MTR_1g017990 | 91.438 | 292 | 17 | 2 | 1 | 291 | 1 | 285 | 0.0 | 534 |
| MS.gene66463.t1 | MTR_3g103190 | 42.672 | 232 | 96 | 8 | 1 | 207 | 1 | 220 | 8.23e-47 | 157 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66463.t1 | AT1G59590 | 40.212 | 189 | 102 | 5 | 22 | 202 | 7 | 192 | 7.93e-33 | 120 |
| MS.gene66463.t1 | AT1G10220 | 40.000 | 180 | 95 | 5 | 22 | 190 | 3 | 180 | 1.73e-25 | 100 |
| MS.gene66463.t1 | AT1G10220 | 37.696 | 191 | 95 | 5 | 22 | 190 | 3 | 191 | 5.92e-24 | 97.1 |
Find 41 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTGTTGTGATAATGATTT+TGG | 0.226913 | 1.4:-4959868 | MS.gene66463:CDS |
| TCTAGGCCACGTGTGGAATA+AGG | 0.234724 | 1.4:+4960206 | None:intergenic |
| ATTCCAAGAAATTCTCTTCT+TGG | 0.237143 | 1.4:+4959932 | None:intergenic |
| TATCTGATTTAAATGATAAA+AGG | 0.270978 | 1.4:-4960166 | MS.gene66463:CDS |
| CATAGTTCATCATCATCTTC+TGG | 0.290163 | 1.4:+4960287 | None:intergenic |
| TATCTTCTAGGGCTTGAATT+AGG | 0.299114 | 1.4:+4960254 | None:intergenic |
| AGGCTTGAGTGAGAAGAAAA+TGG | 0.318153 | 1.4:-4959724 | MS.gene66463:CDS |
| AACCCAAGAAGAGAATTTCT+TGG | 0.322330 | 1.4:-4959935 | MS.gene66463:CDS |
| GACTTAGGACTCAAGCTTCT+TGG | 0.327238 | 1.4:+4960014 | None:intergenic |
| TCAAAATCACCACCACTTCT+TGG | 0.337591 | 1.4:+4959522 | None:intergenic |
| TTCCAAGAAATTCTCTTCTT+GGG | 0.377178 | 1.4:+4959933 | None:intergenic |
| AAGATGGAGTGAGAAGAAAA+TGG | 0.393067 | 1.4:-4959571 | MS.gene66463:CDS |
| AAGAAATTCTCTTCTTGGGT+TGG | 0.410917 | 1.4:+4959937 | None:intergenic |
| TTAAAAGTTCCACAAACAAA+AGG | 0.417291 | 1.4:+4960311 | None:intergenic |
| GATTTAATATCCTTCAAAGT+TGG | 0.427450 | 1.4:+4959780 | None:intergenic |
| AAAATCATTATCACAACAAA+TGG | 0.436688 | 1.4:+4959870 | None:intergenic |
| CTAGGCCACGTGTGGAATAA+GGG | 0.436782 | 1.4:+4960207 | None:intergenic |
| AGAAAGATTATGTTAGAAGA+TGG | 0.449967 | 1.4:-4959587 | MS.gene66463:CDS |
| AACATTCACACAAGAAAAGT+TGG | 0.459312 | 1.4:-4959470 | MS.gene66463:CDS |
| AAAATGGTTACTACTAATGA+TGG | 0.460282 | 1.4:-4959555 | MS.gene66463:CDS |
| GAATATGAAGAATATTGAAA+TGG | 0.462608 | 1.4:-4959913 | MS.gene66463:CDS |
| AGGGTTTAGTGAAAAGAAGA+TGG | 0.477859 | 1.4:-4959634 | MS.gene66463:CDS |
| ATGGATGTAGTGAATAATGA+AGG | 0.481019 | 1.4:-4959705 | MS.gene66463:CDS |
| AAAGTGCTGAAAATTTATCT+AGG | 0.485667 | 1.4:+4960189 | None:intergenic |
| GTATGTTATTCCAACTTTGA+AGG | 0.503391 | 1.4:-4959790 | MS.gene66463:CDS |
| TAAAAGTTCCACAAACAAAA+GGG | 0.520070 | 1.4:+4960312 | None:intergenic |
| TGAATCATCAATTGTTGCTA+TGG | 0.523729 | 1.4:-4959970 | MS.gene66463:CDS |
| AAGAAGATGGTGGTTAGTGA+AGG | 0.545224 | 1.4:-4959621 | MS.gene66463:CDS |
| AGAAGATGGTGGTTAGTGAA+GGG | 0.582106 | 1.4:-4959620 | MS.gene66463:CDS |
| GGATTAGTTTCTCCAAGAAG+TGG | 0.582854 | 1.4:-4959534 | MS.gene66463:CDS |
| GTTTAGTGAAAAGAAGATGG+TGG | 0.609161 | 1.4:-4959631 | MS.gene66463:CDS |
| CGAACAAAAGAAATATCATG+AGG | 0.627048 | 1.4:+4960119 | None:intergenic |
| AAGCTTGAGTCCTAAGTCAA+CGG | 0.627738 | 1.4:-4960009 | MS.gene66463:CDS |
| TTAGTTTCTCCAAGAAGTGG+TGG | 0.628810 | 1.4:-4959531 | MS.gene66463:CDS |
| AAATTTATCTAGGCCACGTG+TGG | 0.632904 | 1.4:+4960199 | None:intergenic |
| ACAAATGGCAAATAGAACAA+TGG | 0.651573 | 1.4:+4959885 | None:intergenic |
| GAACAAAAGAAATATCATGA+GGG | 0.654561 | 1.4:+4960120 | None:intergenic |
| TGGATGTAGTGAATAATGAA+GGG | 0.664223 | 1.4:-4959704 | MS.gene66463:CDS |
| TGTAGTGAATAATGAAGGGG+TGG | 0.690570 | 1.4:-4959700 | MS.gene66463:CDS |
| AATAACCCTTATTCCACACG+TGG | 0.697457 | 1.4:-4960212 | MS.gene66463:CDS |
| GGATGTAGTGAATAATGAAG+GGG | 0.711044 | 1.4:-4959703 | MS.gene66463:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATCTGATTTAAATGATAAA+AGG | - | chr1.4:4959621-4959640 | MS.gene66463:CDS | 15.0% |
| !! | AAAATCATTATCACAACAAA+TGG | + | chr1.4:4959920-4959939 | None:intergenic | 20.0% |
| !! | AAGAAAAAAGATTATGTTAG+AGG | - | chr1.4:4960043-4960062 | MS.gene66463:CDS | 20.0% |
| !! | AGAAAAAAGATTATGTTAGA+GGG | - | chr1.4:4960044-4960063 | MS.gene66463:CDS | 20.0% |
| !! | GAATATGAAGAATATTGAAA+TGG | - | chr1.4:4959874-4959893 | MS.gene66463:CDS | 20.0% |
| !!! | ATTTGTTGTGATAATGATTT+TGG | - | chr1.4:4959919-4959938 | MS.gene66463:CDS | 20.0% |
| !!! | TAATTTTCACTTGTATTTTG+TGG | - | chr1.4:4959975-4959994 | MS.gene66463:CDS | 20.0% |
| ! | AAAATGGTTACTACTAATGA+TGG | - | chr1.4:4960232-4960251 | MS.gene66463:CDS | 25.0% |
| ! | AGAAAGATTATGTTAGAAGA+TGG | - | chr1.4:4960200-4960219 | MS.gene66463:CDS | 25.0% |
| ! | GAACAAAAGAAATATCATGA+GGG | + | chr1.4:4959670-4959689 | None:intergenic | 25.0% |
| ! | GATTTAATATCCTTCAAAGT+TGG | + | chr1.4:4960010-4960029 | None:intergenic | 25.0% |
| ! | TAAAAGTTCCACAAACAAAA+GGG | + | chr1.4:4959478-4959497 | None:intergenic | 25.0% |
| ! | TTAAAAGTTCCACAAACAAA+AGG | + | chr1.4:4959479-4959498 | None:intergenic | 25.0% |
| !! | CTTTTCTTTTGTATCTTCTA+GGG | + | chr1.4:4959547-4959566 | None:intergenic | 25.0% |
| !! | TCTTTTCTTTTGTATCTTCT+AGG | + | chr1.4:4959548-4959567 | None:intergenic | 25.0% |
| !! | TTTTTCACCTTAACAACAAT+AGG | + | chr1.4:4959725-4959744 | None:intergenic | 25.0% |
| !!! | AAAGTGCTGAAAATTTATCT+AGG | + | chr1.4:4959601-4959620 | None:intergenic | 25.0% |
| !!! | TTTTGTTCCTATTGTTGTTA+AGG | - | chr1.4:4959715-4959734 | MS.gene66463:CDS | 25.0% |
| AACATTCACACAAGAAAAGT+TGG | - | chr1.4:4960317-4960336 | MS.gene66463:CDS | 30.0% | |
| ACAAATGGCAAATAGAACAA+TGG | + | chr1.4:4959905-4959924 | None:intergenic | 30.0% | |
| ATGGATGTAGTGAATAATGA+AGG | - | chr1.4:4960082-4960101 | MS.gene66463:CDS | 30.0% | |
| ATTCCAAGAAATTCTCTTCT+TGG | + | chr1.4:4959858-4959877 | None:intergenic | 30.0% | |
| CGAACAAAAGAAATATCATG+AGG | + | chr1.4:4959671-4959690 | None:intergenic | 30.0% | |
| GTATGTTATTCCAACTTTGA+AGG | - | chr1.4:4959997-4960016 | MS.gene66463:CDS | 30.0% | |
| TGAATCATCAATTGTTGCTA+TGG | - | chr1.4:4959817-4959836 | MS.gene66463:CDS | 30.0% | |
| TGGATGTAGTGAATAATGAA+GGG | - | chr1.4:4960083-4960102 | MS.gene66463:CDS | 30.0% | |
| TTCCAAGAAATTCTCTTCTT+GGG | + | chr1.4:4959857-4959876 | None:intergenic | 30.0% | |
| ! | TTTTGCTTGAATCTTGTTCT+TGG | + | chr1.4:4960299-4960318 | None:intergenic | 30.0% |
| !! | GTTTCACTACTTTTAACTCT+TGG | + | chr1.4:4959754-4959773 | None:intergenic | 30.0% |
| AACCCAAGAAGAGAATTTCT+TGG | - | chr1.4:4959852-4959871 | MS.gene66463:CDS | 35.0% | |
| AAGAAATTCTCTTCTTGGGT+TGG | + | chr1.4:4959853-4959872 | None:intergenic | 35.0% | |
| AAGATGGAGTGAGAAGAAAA+TGG | - | chr1.4:4960216-4960235 | MS.gene66463:CDS | 35.0% | |
| AGGGTTTAGTGAAAAGAAGA+TGG | - | chr1.4:4960153-4960172 | MS.gene66463:CDS | 35.0% | |
| CATAGTTCATCATCATCTTC+TGG | + | chr1.4:4959503-4959522 | None:intergenic | 35.0% | |
| GGATGTAGTGAATAATGAAG+GGG | - | chr1.4:4960084-4960103 | MS.gene66463:CDS | 35.0% | |
| GTTTAGTGAAAAGAAGATGG+TGG | - | chr1.4:4960156-4960175 | MS.gene66463:CDS | 35.0% | |
| !! | ATGTTGAACCCTTTTGTTTG+TGG | - | chr1.4:4959467-4959486 | MS.gene66463:CDS | 35.0% |
| !! | TATCTTCTAGGGCTTGAATT+AGG | + | chr1.4:4959536-4959555 | None:intergenic | 35.0% |
| !!! | TTGGTTTTGTTGACTGTGTT+TGG | - | chr1.4:4959938-4959957 | MS.gene66463:CDS | 35.0% |
| AAATTTATCTAGGCCACGTG+TGG | + | chr1.4:4959591-4959610 | None:intergenic | 40.0% | |
| AAGCTTGAGTCCTAAGTCAA+CGG | - | chr1.4:4959778-4959797 | MS.gene66463:CDS | 40.0% | |
| AATAACCCTTATTCCACACG+TGG | - | chr1.4:4959575-4959594 | MS.gene66463:CDS | 40.0% | |
| AGGCTTGAGTGAGAAGAAAA+TGG | - | chr1.4:4960063-4960082 | MS.gene66463:CDS | 40.0% | |
| GGGTGGTGAAGAAAAATGAA+GGG | - | chr1.4:4960104-4960123 | MS.gene66463:CDS | 40.0% | |
| GGTGGTGAAGAAAAATGAAG+GGG | - | chr1.4:4960105-4960124 | MS.gene66463:CDS | 40.0% | |
| GTGAAGAAAAATGAAGGGGT+GGG | - | chr1.4:4960109-4960128 | MS.gene66463:CDS | 40.0% | |
| TCAAAATCACCACCACTTCT+TGG | + | chr1.4:4960268-4960287 | None:intergenic | 40.0% | |
| TGAAGAAAAATGAAGGGGTG+GGG | - | chr1.4:4960110-4960129 | MS.gene66463:CDS | 40.0% | |
| TGTAGTGAATAATGAAGGGG+TGG | - | chr1.4:4960087-4960106 | MS.gene66463:CDS | 40.0% | |
| TTAGTTTCTCCAAGAAGTGG+TGG | - | chr1.4:4960256-4960275 | MS.gene66463:CDS | 40.0% | |
| ! | AAGAAGATGGTGGTTAGTGA+AGG | - | chr1.4:4960166-4960185 | MS.gene66463:CDS | 40.0% |
| ! | AGAAGATGGTGGTTAGTGAA+GGG | - | chr1.4:4960167-4960186 | MS.gene66463:CDS | 40.0% |
| !! | GGATTAGTTTCTCCAAGAAG+TGG | - | chr1.4:4960253-4960272 | MS.gene66463:CDS | 40.0% |
| !!! | GACTTTTTCTCCGTTGACTT+AGG | + | chr1.4:4959791-4959810 | None:intergenic | 40.0% |
| GACTTAGGACTCAAGCTTCT+TGG | + | chr1.4:4959776-4959795 | None:intergenic | 45.0% | |
| GGGGTGGTGAAGAAAAATGA+AGG | - | chr1.4:4960103-4960122 | MS.gene66463:CDS | 45.0% | |
| GGTGAAGAAAAATGAAGGGG+TGG | - | chr1.4:4960108-4960127 | MS.gene66463:CDS | 45.0% | |
| CTAGGCCACGTGTGGAATAA+GGG | + | chr1.4:4959583-4959602 | None:intergenic | 50.0% | |
| TCTAGGCCACGTGTGGAATA+AGG | + | chr1.4:4959584-4959603 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 4959467 | 4960342 | 4959467 | ID=MS.gene66463 |
| chr1.4 | mRNA | 4959467 | 4960342 | 4959467 | ID=MS.gene66463.t1;Parent=MS.gene66463 |
| chr1.4 | exon | 4959467 | 4960342 | 4959467 | ID=MS.gene66463.t1.exon1;Parent=MS.gene66463.t1 |
| chr1.4 | CDS | 4959467 | 4960342 | 4959467 | ID=cds.MS.gene66463.t1;Parent=MS.gene66463.t1 |
| Gene Sequence |
| Protein sequence |
