Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66627.t1 | XP_024637566.1 | 96 | 100 | 1 | 1 | 1 | 100 | 51 | 147 | 2.10E-42 | 182.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66627.t1 | Q9XFB0 | 60.5 | 86 | 30 | 2 | 15 | 100 | 68 | 149 | 2.4e-18 | 93.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66627.t1 | A0A396I9S2 | 96.0 | 100 | 1 | 1 | 1 | 100 | 51 | 147 | 1.5e-42 | 182.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene66627.t1 | TF | C2C2-YABBY |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049369 | MS.gene66627 | 0.813181 | 2.91E-51 | -1.69E-46 |
| MS.gene049427 | MS.gene66627 | 0.800996 | 1.11E-48 | -1.69E-46 |
| MS.gene049491 | MS.gene66627 | 0.805148 | 1.54E-49 | -1.69E-46 |
| MS.gene049614 | MS.gene66627 | 0.823925 | 1.07E-53 | -1.69E-46 |
| MS.gene049661 | MS.gene66627 | 0.804996 | 1.65E-49 | -1.69E-46 |
| MS.gene050083 | MS.gene66627 | -0.80144 | 9.00E-49 | -1.69E-46 |
| MS.gene050397 | MS.gene66627 | 0.82921 | 5.85E-55 | -1.69E-46 |
| MS.gene050767 | MS.gene66627 | 0.813982 | 1.94E-51 | -1.69E-46 |
| MS.gene050770 | MS.gene66627 | 0.815939 | 7.15E-52 | -1.69E-46 |
| MS.gene05101 | MS.gene66627 | 0.80755 | 4.79E-50 | -1.69E-46 |
| MS.gene051228 | MS.gene66627 | 0.808101 | 3.65E-50 | -1.69E-46 |
| MS.gene051692 | MS.gene66627 | 0.819138 | 1.36E-52 | -1.69E-46 |
| MS.gene051858 | MS.gene66627 | 0.803109 | 4.08E-49 | -1.69E-46 |
| MS.gene052078 | MS.gene66627 | 0.802904 | 4.50E-49 | -1.69E-46 |
| MS.gene052178 | MS.gene66627 | 0.802294 | 6.01E-49 | -1.69E-46 |
| MS.gene052271 | MS.gene66627 | 0.816701 | 4.83E-52 | -1.69E-46 |
| MS.gene052290 | MS.gene66627 | 0.815456 | 9.16E-52 | -1.69E-46 |
| MS.gene052310 | MS.gene66627 | 0.803942 | 2.74E-49 | -1.69E-46 |
| MS.gene052326 | MS.gene66627 | 0.805163 | 1.52E-49 | -1.69E-46 |
| MS.gene052379 | MS.gene66627 | 0.819517 | 1.11E-52 | -1.69E-46 |
| MS.gene052484 | MS.gene66627 | 0.80637 | 8.50E-50 | -1.69E-46 |
| MS.gene053104 | MS.gene66627 | 0.813726 | 2.21E-51 | -1.69E-46 |
| MS.gene053209 | MS.gene66627 | 0.818735 | 1.68E-52 | -1.69E-46 |
| MS.gene053322 | MS.gene66627 | 0.833402 | 5.44E-56 | -1.69E-46 |
| MS.gene053449 | MS.gene66627 | 0.818865 | 1.57E-52 | -1.69E-46 |
| MS.gene053813 | MS.gene66627 | 0.813226 | 2.85E-51 | -1.69E-46 |
| MS.gene054376 | MS.gene66627 | 0.811382 | 7.20E-51 | -1.69E-46 |
| MS.gene054802 | MS.gene66627 | 0.807551 | 4.78E-50 | -1.69E-46 |
| MS.gene054803 | MS.gene66627 | 0.805936 | 1.05E-49 | -1.69E-46 |
| MS.gene054804 | MS.gene66627 | 0.807861 | 4.11E-50 | -1.69E-46 |
| MS.gene055254 | MS.gene66627 | 0.809162 | 2.17E-50 | -1.69E-46 |
| MS.gene055277 | MS.gene66627 | 0.838666 | 2.51E-57 | -1.69E-46 |
| MS.gene055397 | MS.gene66627 | 0.806395 | 8.40E-50 | -1.69E-46 |
| MS.gene055499 | MS.gene66627 | 0.831255 | 1.85E-55 | -1.69E-46 |
| MS.gene05592 | MS.gene66627 | 0.823165 | 1.60E-53 | -1.69E-46 |
| MS.gene056191 | MS.gene66627 | 0.833219 | 6.05E-56 | -1.69E-46 |
| MS.gene056410 | MS.gene66627 | 0.869683 | 2.58E-66 | -1.69E-46 |
| MS.gene056609 | MS.gene66627 | 0.851565 | 8.17E-61 | -1.69E-46 |
| MS.gene056611 | MS.gene66627 | 0.836532 | 8.86E-57 | -1.69E-46 |
| MS.gene056897 | MS.gene66627 | 0.808324 | 3.28E-50 | -1.69E-46 |
| MS.gene057129 | MS.gene66627 | 0.816519 | 5.30E-52 | -1.69E-46 |
| MS.gene057796 | MS.gene66627 | 0.811098 | 8.30E-51 | -1.69E-46 |
| MS.gene057926 | MS.gene66627 | 0.812777 | 3.57E-51 | -1.69E-46 |
| MS.gene058588 | MS.gene66627 | 0.807875 | 4.08E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene66627 | 0.833378 | 5.52E-56 | -1.69E-46 |
| MS.gene058928 | MS.gene66627 | 0.819875 | 9.23E-53 | -1.69E-46 |
| MS.gene05907 | MS.gene66627 | 0.810504 | 1.12E-50 | -1.69E-46 |
| MS.gene059294 | MS.gene66627 | 0.834085 | 3.68E-56 | -1.69E-46 |
| MS.gene059536 | MS.gene66627 | 0.849995 | 2.26E-60 | -1.69E-46 |
| MS.gene059676 | MS.gene66627 | 0.806783 | 6.96E-50 | -1.69E-46 |
| MS.gene059688 | MS.gene66627 | 0.803608 | 3.21E-49 | -1.69E-46 |
| MS.gene059692 | MS.gene66627 | 0.812718 | 3.68E-51 | -1.69E-46 |
| MS.gene059695 | MS.gene66627 | 0.803716 | 3.05E-49 | -1.69E-46 |
| MS.gene059708 | MS.gene66627 | 0.8034 | 3.55E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene66627 | 0.804298 | 2.31E-49 | -1.69E-46 |
| MS.gene059902 | MS.gene66627 | 0.803015 | 4.26E-49 | -1.69E-46 |
| MS.gene059960 | MS.gene66627 | 0.808352 | 3.23E-50 | -1.69E-46 |
| MS.gene059974 | MS.gene66627 | 0.828285 | 9.79E-55 | -1.69E-46 |
| MS.gene060005 | MS.gene66627 | 0.811113 | 8.23E-51 | -1.69E-46 |
| MS.gene060804 | MS.gene66627 | 0.835244 | 1.88E-56 | -1.69E-46 |
| MS.gene060818 | MS.gene66627 | 0.83352 | 5.09E-56 | -1.69E-46 |
| MS.gene060837 | MS.gene66627 | 0.80482 | 1.80E-49 | -1.69E-46 |
| MS.gene061041 | MS.gene66627 | 0.82551 | 4.51E-54 | -1.69E-46 |
| MS.gene061051 | MS.gene66627 | -0.816411 | 5.61E-52 | -1.69E-46 |
| MS.gene061200 | MS.gene66627 | 0.828788 | 7.40E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66627.t1 | MTR_4g050300 | 94.118 | 102 | 1 | 2 | 1 | 100 | 51 | 149 | 5.42e-59 | 182 |
| MS.gene66627.t1 | MTR_2g087740 | 67.290 | 107 | 27 | 4 | 1 | 100 | 54 | 159 | 1.05e-31 | 113 |
| MS.gene66627.t1 | MTR_4g114730 | 67.143 | 70 | 22 | 1 | 32 | 100 | 84 | 153 | 2.27e-19 | 81.3 |
| MS.gene66627.t1 | MTR_5g034030 | 47.826 | 92 | 38 | 2 | 14 | 100 | 71 | 157 | 8.45e-18 | 77.8 |
| MS.gene66627.t1 | MTR_4g025900 | 90.000 | 40 | 4 | 0 | 61 | 100 | 135 | 174 | 1.29e-17 | 77.4 |
| MS.gene66627.t1 | MTR_5g046230 | 72.549 | 51 | 12 | 1 | 52 | 100 | 101 | 151 | 3.00e-17 | 76.3 |
| MS.gene66627.t1 | MTR_4g101660 | 85.000 | 40 | 6 | 0 | 61 | 100 | 130 | 169 | 2.47e-16 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66627.t1 | AT1G08465 | 60.465 | 86 | 30 | 2 | 15 | 100 | 68 | 149 | 2.91e-25 | 96.3 |
| MS.gene66627.t1 | AT2G45190 | 72.727 | 66 | 16 | 2 | 37 | 100 | 49 | 114 | 8.30e-23 | 89.7 |
| MS.gene66627.t1 | AT2G45190 | 73.846 | 65 | 15 | 2 | 38 | 100 | 114 | 178 | 3.61e-22 | 89.4 |
| MS.gene66627.t1 | AT2G26580 | 66.667 | 72 | 23 | 1 | 29 | 100 | 72 | 142 | 7.93e-19 | 79.3 |
| MS.gene66627.t1 | AT2G26580 | 66.667 | 72 | 23 | 1 | 29 | 100 | 72 | 142 | 7.93e-19 | 79.3 |
| MS.gene66627.t1 | AT4G00180 | 92.500 | 40 | 3 | 0 | 61 | 100 | 120 | 159 | 3.56e-18 | 78.6 |
| MS.gene66627.t1 | AT4G00180 | 92.500 | 40 | 3 | 0 | 61 | 100 | 151 | 190 | 7.72e-18 | 78.2 |
| MS.gene66627.t1 | AT1G69180 | 68.182 | 44 | 14 | 0 | 57 | 100 | 109 | 152 | 2.04e-15 | 70.5 |
| MS.gene66627.t1 | AT1G23420 | 69.767 | 43 | 13 | 0 | 58 | 100 | 130 | 172 | 8.00e-13 | 64.7 |
| MS.gene66627.t1 | AT1G23420 | 69.767 | 43 | 13 | 0 | 58 | 100 | 161 | 203 | 1.28e-12 | 64.3 |
Find 44 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGCTGATATCTGGATTAC+TGG | 0.287474 | 4.3:-49461661 | None:intergenic |
| GCCTCCCTGTGGCTGATATC+TGG | 0.289400 | 4.3:-49461669 | None:intergenic |
| TATGCGGTTGAGCAGATTTA+CGG | 0.342589 | 4.3:+49466570 | MS.gene66627:CDS |
| GAAGCAAGCAGCAAGGAAAT+TGG | 0.345303 | 4.3:+49460166 | MS.gene66627:CDS |
| CAGTGGTCTTGTACAATAAT+TGG | 0.349086 | 4.3:-49472063 | None:intergenic |
| GGAGGAAATTCAAAGGATTA+AGG | 0.360502 | 4.3:+49461638 | MS.gene66627:CDS |
| TTCTAGATACATCTTGTGTT+TGG | 0.377513 | 4.3:-49472035 | None:intergenic |
| GATACATCTTGTGTTTGGTT+TGG | 0.416418 | 4.3:-49472030 | None:intergenic |
| ATATAACTTACTTAATGAAC+CGG | 0.422652 | 4.3:-49461280 | None:intergenic |
| TACTGCAAACCGTTCAAAAC+CGG | 0.430848 | 4.3:-49466593 | None:intergenic |
| GGGAGTTGAGGAGGAGGAAA+TGG | 0.440779 | 4.3:-49458260 | None:intergenic |
| ATCTGATCTCACAATCGGTC+TGG | 0.440792 | 4.3:-49466531 | None:intergenic |
| CGACGAATGGGAGGAATCCT+AGG | 0.445790 | 4.3:-49460250 | None:intergenic |
| GTGTTCCTTCTGCTTACAAC+CGG | 0.449198 | 4.3:+49461261 | MS.gene66627:CDS |
| TCCTCCTCCTCAACTCCCTC+AGG | 0.474865 | 4.3:+49458265 | MS.gene66627:CDS |
| TACAAGACCACTGCACTAGC+AGG | 0.475632 | 4.3:+49472073 | MS.gene66627:CDS |
| CAACAAAATAGCATCATTTG+AGG | 0.493091 | 4.3:+49460207 | MS.gene66627:CDS |
| AAACTCGAACAAATATGAAA+AGG | 0.514523 | 4.3:+49466491 | MS.gene66627:CDS |
| AGAGTGTGACTCACGACGAA+TGG | 0.516345 | 4.3:-49460263 | None:intergenic |
| TAGTAGAGCACGAACATCCT+AGG | 0.516780 | 4.3:+49460233 | MS.gene66627:CDS |
| GAGTGTGACTCACGACGAAT+GGG | 0.531160 | 4.3:-49460262 | None:intergenic |
| GACCAATCTGATCTCACAAT+CGG | 0.531315 | 4.3:-49466536 | None:intergenic |
| ACCTGAGGGAGTTGAGGAGG+AGG | 0.539948 | 4.3:-49458266 | None:intergenic |
| ATCTCACAATCGGTCTGGAA+TGG | 0.540798 | 4.3:-49466526 | None:intergenic |
| GGAGGAAATGGCTGAAGTAA+TGG | 0.547935 | 4.3:-49458248 | None:intergenic |
| GATTGTGAGATCAGATTGGT+CGG | 0.554823 | 4.3:+49466538 | MS.gene66627:CDS |
| CTTTCACCCTGCTAGTGCAG+TGG | 0.566015 | 4.3:-49472080 | None:intergenic |
| AAACATACCTGAGGGAGTTG+AGG | 0.577695 | 4.3:-49458272 | None:intergenic |
| ATGAACCGGTTGTAAGCAGA+AGG | 0.582569 | 4.3:-49461266 | None:intergenic |
| TGGTCGGGTGGCAGTTTATG+CGG | 0.584948 | 4.3:+49466554 | MS.gene66627:CDS |
| GTAATCCAGATATCAGCCAC+AGG | 0.588300 | 4.3:+49461664 | MS.gene66627:CDS |
| ATTGTGAGATCAGATTGGTC+GGG | 0.593034 | 4.3:+49466539 | MS.gene66627:CDS |
| GACCGATTGTGAGATCAGAT+TGG | 0.593523 | 4.3:+49466534 | MS.gene66627:CDS |
| GTGAGATCAGATTGGTCGGG+TGG | 0.621943 | 4.3:+49466542 | MS.gene66627:CDS |
| TCTGTAGGGAGGAAATTCAA+AGG | 0.621966 | 4.3:+49461631 | MS.gene66627:intron |
| CTGAGCTGAAAGCCTCCCTG+TGG | 0.623676 | 4.3:-49461680 | None:intergenic |
| CAGGGAGGCTTTCAGCTCAG+CGG | 0.634037 | 4.3:+49461683 | MS.gene66627:CDS |
| CATACCTGAGGGAGTTGAGG+AGG | 0.635233 | 4.3:-49458269 | None:intergenic |
| ACAAGACCACTGCACTAGCA+GGG | 0.642198 | 4.3:+49472074 | MS.gene66627:CDS |
| TGTGACTCACGACGAATGGG+AGG | 0.645995 | 4.3:-49460259 | None:intergenic |
| CGGTTGAGCAGATTTACGGC+CGG | 0.652921 | 4.3:+49466574 | MS.gene66627:CDS |
| TCATGAAGAAGCAAGCAGCA+AGG | 0.659976 | 4.3:+49460159 | MS.gene66627:CDS |
| TCCAGATATCAGCCACAGGG+AGG | 0.683704 | 4.3:+49461668 | MS.gene66627:CDS |
| TAATCCAGATATCAGCCACA+GGG | 0.696359 | 4.3:+49461665 | MS.gene66627:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 49458239 | 49472116 | 49458239 | ID=MS.gene66627 |
| chr4.3 | mRNA | 49458239 | 49472116 | 49458239 | ID=MS.gene66627.t1;Parent=MS.gene66627 |
| chr4.3 | exon | 49458239 | 49458286 | 49458239 | ID=MS.gene66627.t1.exon1;Parent=MS.gene66627.t1 |
| chr4.3 | CDS | 49458239 | 49458286 | 49458239 | ID=cds.MS.gene66627.t1;Parent=MS.gene66627.t1 |
| chr4.3 | exon | 49460145 | 49460271 | 49460145 | ID=MS.gene66627.t1.exon2;Parent=MS.gene66627.t1 |
| chr4.3 | CDS | 49460145 | 49460271 | 49460145 | ID=cds.MS.gene66627.t1;Parent=MS.gene66627.t1 |
| chr4.3 | exon | 49461243 | 49461291 | 49461243 | ID=MS.gene66627.t1.exon3;Parent=MS.gene66627.t1 |
| chr4.3 | CDS | 49461243 | 49461291 | 49461243 | ID=cds.MS.gene66627.t1;Parent=MS.gene66627.t1 |
| chr4.3 | exon | 49461638 | 49461713 | 49461638 | ID=MS.gene66627.t1.exon4;Parent=MS.gene66627.t1 |
| chr4.3 | CDS | 49461638 | 49461713 | 49461638 | ID=cds.MS.gene66627.t1;Parent=MS.gene66627.t1 |
| chr4.3 | exon | 49466481 | 49466605 | 49466481 | ID=MS.gene66627.t1.exon5;Parent=MS.gene66627.t1 |
| chr4.3 | CDS | 49466481 | 49466605 | 49466481 | ID=cds.MS.gene66627.t1;Parent=MS.gene66627.t1 |
| chr4.3 | exon | 49472014 | 49472116 | 49472014 | ID=MS.gene66627.t1.exon6;Parent=MS.gene66627.t1 |
| chr4.3 | CDS | 49472014 | 49472116 | 49472014 | ID=cds.MS.gene66627.t1;Parent=MS.gene66627.t1 |
| Gene Sequence |
| Protein sequence |
