Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71940.t1 | XP_003608722.2 | 66.5 | 236 | 19 | 2 | 1 | 176 | 1 | 236 | 2.00E-53 | 218.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71940.t1 | G7JIX6 | 66.5 | 236 | 19 | 2 | 1 | 176 | 1 | 236 | 1.5e-53 | 218.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene71940 | 0.803309 | 3.71E-49 | -1.69E-46 |
| MS.gene04919 | MS.gene71940 | 0.810758 | 9.83E-51 | -1.69E-46 |
| MS.gene04920 | MS.gene71940 | 0.829796 | 4.21E-55 | -1.69E-46 |
| MS.gene049254 | MS.gene71940 | 0.804891 | 1.74E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene71940 | 0.821125 | 4.77E-53 | -1.69E-46 |
| MS.gene049419 | MS.gene71940 | -0.830069 | 3.61E-55 | -1.69E-46 |
| MS.gene049499 | MS.gene71940 | 0.841428 | 4.78E-58 | -1.69E-46 |
| MS.gene049983 | MS.gene71940 | 0.837947 | 3.85E-57 | -1.69E-46 |
| MS.gene050654 | MS.gene71940 | 0.815092 | 1.10E-51 | -1.69E-46 |
| MS.gene050737 | MS.gene71940 | 0.828435 | 9.00E-55 | -1.69E-46 |
| MS.gene050759 | MS.gene71940 | 0.801406 | 9.14E-49 | -1.69E-46 |
| MS.gene050941 | MS.gene71940 | 0.852002 | 6.14E-61 | -1.69E-46 |
| MS.gene051122 | MS.gene71940 | 0.801896 | 7.25E-49 | -1.69E-46 |
| MS.gene051275 | MS.gene71940 | 0.803175 | 3.95E-49 | -1.69E-46 |
| MS.gene051541 | MS.gene71940 | 0.804966 | 1.68E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene71940 | 0.812266 | 4.62E-51 | -1.69E-46 |
| MS.gene052493 | MS.gene71940 | 0.810574 | 1.08E-50 | -1.69E-46 |
| MS.gene052518 | MS.gene71940 | 0.805606 | 1.23E-49 | -1.69E-46 |
| MS.gene052674 | MS.gene71940 | 0.820794 | 5.68E-53 | -1.69E-46 |
| MS.gene052675 | MS.gene71940 | 0.81844 | 1.96E-52 | -1.69E-46 |
| MS.gene052677 | MS.gene71940 | 0.81597 | 7.04E-52 | -1.69E-46 |
| MS.gene053006 | MS.gene71940 | 0.8271 | 1.88E-54 | -1.69E-46 |
| MS.gene053726 | MS.gene71940 | 0.83751 | 4.98E-57 | -1.69E-46 |
| MS.gene053963 | MS.gene71940 | 0.811763 | 5.95E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene71940 | 0.849354 | 3.41E-60 | -1.69E-46 |
| MS.gene054892 | MS.gene71940 | 0.833079 | 6.55E-56 | -1.69E-46 |
| MS.gene054893 | MS.gene71940 | 0.837214 | 5.93E-57 | -1.69E-46 |
| MS.gene055724 | MS.gene71940 | 0.825211 | 5.31E-54 | -1.69E-46 |
| MS.gene055841 | MS.gene71940 | 0.803524 | 3.35E-49 | -1.69E-46 |
| MS.gene055864 | MS.gene71940 | 0.803973 | 2.70E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene71940 | 0.814823 | 1.27E-51 | -1.69E-46 |
| MS.gene056699 | MS.gene71940 | 0.83069 | 2.55E-55 | -1.69E-46 |
| MS.gene056776 | MS.gene71940 | 0.820869 | 5.46E-53 | -1.69E-46 |
| MS.gene056779 | MS.gene71940 | 0.814717 | 1.34E-51 | -1.69E-46 |
| MS.gene056787 | MS.gene71940 | 0.817791 | 2.75E-52 | -1.69E-46 |
| MS.gene05690 | MS.gene71940 | 0.80566 | 1.20E-49 | -1.69E-46 |
| MS.gene057155 | MS.gene71940 | 0.857631 | 1.43E-62 | -1.69E-46 |
| MS.gene057208 | MS.gene71940 | 0.818441 | 1.96E-52 | -1.69E-46 |
| MS.gene057371 | MS.gene71940 | 0.803068 | 4.16E-49 | -1.69E-46 |
| MS.gene057622 | MS.gene71940 | 0.800844 | 1.19E-48 | -1.69E-46 |
| MS.gene058159 | MS.gene71940 | 0.811072 | 8.41E-51 | -1.69E-46 |
| MS.gene058429 | MS.gene71940 | 0.836062 | 1.17E-56 | -1.69E-46 |
| MS.gene059283 | MS.gene71940 | 0.82491 | 6.25E-54 | -1.69E-46 |
| MS.gene05952 | MS.gene71940 | 0.847563 | 1.07E-59 | -1.69E-46 |
| MS.gene059952 | MS.gene71940 | 0.803731 | 3.03E-49 | -1.69E-46 |
| MS.gene060259 | MS.gene71940 | 0.847423 | 1.17E-59 | -1.69E-46 |
| MS.gene060520 | MS.gene71940 | 0.822589 | 2.18E-53 | -1.69E-46 |
| MS.gene060850 | MS.gene71940 | 0.844428 | 7.61E-59 | -1.69E-46 |
| MS.gene060851 | MS.gene71940 | 0.847209 | 1.34E-59 | -1.69E-46 |
| MS.gene060852 | MS.gene71940 | 0.848097 | 7.61E-60 | -1.69E-46 |
| MS.gene060932 | MS.gene71940 | 0.851761 | 7.19E-61 | -1.69E-46 |
| MS.gene060935 | MS.gene71940 | 0.805764 | 1.14E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene71940 | 0.810707 | 1.01E-50 | -1.69E-46 |
| MS.gene06121 | MS.gene71940 | 0.81089 | 9.20E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71940.t1 | MTR_4g101030 | 73.729 | 236 | 2 | 2 | 1 | 176 | 1 | 236 | 2.29e-93 | 271 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71940.t1 | AT4G16535 | 48.913 | 184 | 36 | 4 | 1 | 131 | 1 | 179 | 2.76e-36 | 126 |
Find 37 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGTTTAAGCTGTTCTTCTT+TGG | 0.295938 | 8.3:-20109643 | None:intergenic |
| AATTTATATTTCTCATCTTC+CGG | 0.307288 | 8.3:-20109969 | None:intergenic |
| GTTATGGTGTTGCTGGGAAA+GGG | 0.314214 | 8.3:+20109994 | MS.gene71940:CDS |
| GAAGATGAGAAATATAAATT+AGG | 0.320007 | 8.3:+20109972 | MS.gene71940:CDS |
| CTCGACGACTTTGTTCCTTC+GGG | 0.345048 | 8.3:-20110208 | None:intergenic |
| TGAGGGAAGAGAGGTTGAAA+AGG | 0.347869 | 8.3:+20110144 | MS.gene71940:CDS |
| AAATTAGGTTATGGTGTTGC+TGG | 0.350657 | 8.3:+20109987 | MS.gene71940:CDS |
| TCTCGACGACTTTGTTCCTT+CGG | 0.381444 | 8.3:-20110209 | None:intergenic |
| GAGAAATATAAATTAGGTTA+TGG | 0.398827 | 8.3:+20109978 | MS.gene71940:CDS |
| GGTTATGGTGTTGCTGGGAA+AGG | 0.406889 | 8.3:+20109993 | MS.gene71940:CDS |
| GAAGACATTGGAAGAGTTGA+GGG | 0.423090 | 8.3:+20110127 | MS.gene71940:CDS |
| GGTGAGACTGTTCGTGCTGT+TGG | 0.427915 | 8.3:+20109945 | MS.gene71940:CDS |
| CGGCGGCTTCTTCGTCTTTG+CGG | 0.430480 | 8.3:-20109620 | None:intergenic |
| GAGGGAAGAGAGGTTGAAAA+GGG | 0.431550 | 8.3:+20110145 | MS.gene71940:CDS |
| TTATGGTGTTGCTGGGAAAG+GGG | 0.455179 | 8.3:+20109995 | MS.gene71940:CDS |
| GAACAACTCCGTGTTGCTAA+AGG | 0.455180 | 8.3:+20109702 | MS.gene71940:CDS |
| TGGAAGAGTTGAGGGAAGAG+AGG | 0.459509 | 8.3:+20110135 | MS.gene71940:CDS |
| GGGAGAAGAAAGAGAAGGAG+AGG | 0.475130 | 8.3:+20110165 | MS.gene71940:CDS |
| GGTCATATTAATTTATTCAA+AGG | 0.480959 | 8.3:+20109819 | MS.gene71940:intron |
| AATTAGGTTATGGTGTTGCT+GGG | 0.492710 | 8.3:+20109988 | MS.gene71940:CDS |
| GGAAAGGGGTTGAATTGCCT+TGG | 0.497813 | 8.3:+20110009 | MS.gene71940:CDS |
| AGAAGACATTGGAAGAGTTG+AGG | 0.503007 | 8.3:+20110126 | MS.gene71940:CDS |
| TGAGAAAAGGAAGAAGACAT+TGG | 0.509644 | 8.3:+20110115 | MS.gene71940:intron |
| ATTATAAGAAGTTGAAGATG+AGG | 0.510408 | 8.3:+20109913 | MS.gene71940:CDS |
| GACTGTTCGTGCTGTTGGAC+CGG | 0.511227 | 8.3:+20109950 | MS.gene71940:CDS |
| TTTAAGCTGTTCTTCTTTGG+CGG | 0.512755 | 8.3:-20109640 | None:intergenic |
| GGAGAAGAAAGAGAAGGAGA+GGG | 0.514567 | 8.3:+20110166 | MS.gene71940:CDS |
| GAGTTGTTCAAGACGGACTT+CGG | 0.535233 | 8.3:-20109688 | None:intergenic |
| AAGCTGTTCTTCTTTGGCGG+CGG | 0.548709 | 8.3:-20109637 | None:intergenic |
| TTGAAGATGAGGAAAGAGCC+TGG | 0.554510 | 8.3:+20109924 | MS.gene71940:CDS |
| AGCGTTGATGCGGCACCCGA+AGG | 0.578195 | 8.3:+20110193 | MS.gene71940:CDS |
| GAAAAGGGAGAAGAAAGAGA+AGG | 0.585811 | 8.3:+20110160 | MS.gene71940:CDS |
| TTGCAAGTGATAGATACTAC+AGG | 0.596940 | 8.3:+20110237 | MS.gene71940:CDS |
| AGAGGGAGAGAGCGTTGATG+CGG | 0.601096 | 8.3:+20110183 | MS.gene71940:CDS |
| GTGCCAAACCTTTAGCAACA+CGG | 0.641531 | 8.3:-20109710 | None:intergenic |
| ACAGCACGAACAGTCTCACC+AGG | 0.646246 | 8.3:-20109942 | None:intergenic |
| CAACACGGAGTTGTTCAAGA+CGG | 0.674484 | 8.3:-20109695 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTATATTTCTCATCTTC+CGG | - | chr8.3:20109972-20109991 | None:intergenic | 20.0% |
| !! | GAAGATGAGAAATATAAATT+AGG | + | chr8.3:20109972-20109991 | MS.gene71940:CDS | 20.0% |
| !! | GAGAAATATAAATTAGGTTA+TGG | + | chr8.3:20109978-20109997 | MS.gene71940:CDS | 20.0% |
| !! | GGTCATATTAATTTATTCAA+AGG | + | chr8.3:20109819-20109838 | MS.gene71940:intron | 20.0% |
| ! | ATTATAAGAAGTTGAAGATG+AGG | + | chr8.3:20109913-20109932 | MS.gene71940:CDS | 25.0% |
| ! | AGAGATTGTTGATGAAAAAG+AGG | + | chr8.3:20109878-20109897 | MS.gene71940:CDS | 30.0% |
| ! | TCATCAACAATCTCTCTTTT+CGG | - | chr8.3:20109873-20109892 | None:intergenic | 30.0% |
| ! | TTCAATCATGATGATGATGT+TGG | + | chr8.3:20110050-20110069 | MS.gene71940:intron | 30.0% |
| ! | TTCTTCTTTTGAACATACCA+AGG | - | chr8.3:20110029-20110048 | None:intergenic | 30.0% |
| TGAGAAAAGGAAGAAGACAT+TGG | + | chr8.3:20110115-20110134 | MS.gene71940:intron | 35.0% | |
| TTGCAAGTGATAGATACTAC+AGG | + | chr8.3:20110237-20110256 | MS.gene71940:CDS | 35.0% | |
| TTTAAGCTGTTCTTCTTTGG+CGG | - | chr8.3:20109643-20109662 | None:intergenic | 35.0% | |
| ! | AAACGTTCCAACGTTTTTGA+GGG | - | chr8.3:20109575-20109594 | None:intergenic | 35.0% |
| ! | AATCATGATGATGATGTTGG+TGG | + | chr8.3:20110053-20110072 | MS.gene71940:intron | 35.0% |
| ! | TAAACGTTCCAACGTTTTTG+AGG | - | chr8.3:20109576-20109595 | None:intergenic | 35.0% |
| !! | AAATTAGGTTATGGTGTTGC+TGG | + | chr8.3:20109987-20110006 | MS.gene71940:CDS | 35.0% |
| !! | AATTAGGTTATGGTGTTGCT+GGG | + | chr8.3:20109988-20110007 | MS.gene71940:CDS | 35.0% |
| ACATTCTCCCTCAAAAACGT+TGG | + | chr8.3:20109565-20109584 | MS.gene71940:CDS | 40.0% | |
| AGAAGACATTGGAAGAGTTG+AGG | + | chr8.3:20110126-20110145 | MS.gene71940:CDS | 40.0% | |
| GAAAAGGGAGAAGAAAGAGA+AGG | + | chr8.3:20110160-20110179 | MS.gene71940:CDS | 40.0% | |
| GAAGACATTGGAAGAGTTGA+GGG | + | chr8.3:20110127-20110146 | MS.gene71940:CDS | 40.0% | |
| GCGTTTAAGCTGTTCTTCTT+TGG | - | chr8.3:20109646-20109665 | None:intergenic | 40.0% | |
| ! | TCTCTTTTCGGTACACGAAT+CGG | - | chr8.3:20109861-20109880 | None:intergenic | 40.0% |
| ! | TTCAGCTTCGGATTTAGGTT+TGG | - | chr8.3:20109769-20109788 | None:intergenic | 40.0% |
| ! | TTTAGGTTTGGCTTCAGGTT+CGG | - | chr8.3:20109757-20109776 | None:intergenic | 40.0% |
| !! | TTCGATTTCAGGTTCAGCTT+CGG | - | chr8.3:20109781-20109800 | None:intergenic | 40.0% |
| CAACACGGAGTTGTTCAAGA+CGG | - | chr8.3:20109698-20109717 | None:intergenic | 45.0% | |
| CCTGAAATCGAAGACAAAGC+TGG | + | chr8.3:20109789-20109808 | MS.gene71940:intron | 45.0% | |
| GAGGGAAGAGAGGTTGAAAA+GGG | + | chr8.3:20110145-20110164 | MS.gene71940:CDS | 45.0% | |
| GGAGAAGAAAGAGAAGGAGA+GGG | + | chr8.3:20110166-20110185 | MS.gene71940:CDS | 45.0% | |
| GTGATAGGAAGGGTGAGAAA+AGG | + | chr8.3:20110102-20110121 | MS.gene71940:intron | 45.0% | |
| GTGCCAAACCTTTAGCAACA+CGG | - | chr8.3:20109713-20109732 | None:intergenic | 45.0% | |
| TCAGGTTCAGCTTCGGATTT+AGG | - | chr8.3:20109774-20109793 | None:intergenic | 45.0% | |
| TCTCGACGACTTTGTTCCTT+CGG | - | chr8.3:20110212-20110231 | None:intergenic | 45.0% | |
| TGAGGGAAGAGAGGTTGAAA+AGG | + | chr8.3:20110144-20110163 | MS.gene71940:CDS | 45.0% | |
| TTGAAGATGAGGAAAGAGCC+TGG | + | chr8.3:20109924-20109943 | MS.gene71940:CDS | 45.0% | |
| ! | ACTCCGTGTTGCTAAAGGTT+TGG | + | chr8.3:20109707-20109726 | MS.gene71940:intron | 45.0% |
| ! | CATGATGATGATGTTGGTGG+TGG | + | chr8.3:20110056-20110075 | MS.gene71940:intron | 45.0% |
| ! | CCAGCTTTGTCTTCGATTTC+AGG | - | chr8.3:20109792-20109811 | None:intergenic | 45.0% |
| ! | GAACAACTCCGTGTTGCTAA+AGG | + | chr8.3:20109702-20109721 | MS.gene71940:CDS | 45.0% |
| ! | GAAGACAAAGCTGGTGATGT+AGG | + | chr8.3:20109798-20109817 | MS.gene71940:intron | 45.0% |
| ! | GAGTTGTTCAAGACGGACTT+CGG | - | chr8.3:20109691-20109710 | None:intergenic | 45.0% |
| ! | TCGGATTTAGGTTTGGCTTC+AGG | - | chr8.3:20109762-20109781 | None:intergenic | 45.0% |
| !! | AAATGGTGGTGGTGATAGGA+AGG | + | chr8.3:20110091-20110110 | MS.gene71940:intron | 45.0% |
| !! | AATGGTGGTGGTGATAGGAA+GGG | + | chr8.3:20110092-20110111 | MS.gene71940:intron | 45.0% |
| !! | GTTATGGTGTTGCTGGGAAA+GGG | + | chr8.3:20109994-20110013 | MS.gene71940:CDS | 45.0% |
| !! | TTATGGTGTTGCTGGGAAAG+GGG | + | chr8.3:20109995-20110014 | MS.gene71940:CDS | 45.0% |
| AAGCTGTTCTTCTTTGGCGG+CGG | - | chr8.3:20109640-20109659 | None:intergenic | 50.0% | |
| CTCGACGACTTTGTTCCTTC+GGG | - | chr8.3:20110211-20110230 | None:intergenic | 50.0% | |
| GGAAAGGGGTTGAATTGCCT+TGG | + | chr8.3:20110009-20110028 | MS.gene71940:CDS | 50.0% | |
| GGGAGAAGAAAGAGAAGGAG+AGG | + | chr8.3:20110165-20110184 | MS.gene71940:CDS | 50.0% | |
| TGGAAGAGTTGAGGGAAGAG+AGG | + | chr8.3:20110135-20110154 | MS.gene71940:CDS | 50.0% | |
| ! | TTGGCTTCAGGTTCGGATTC+AGG | - | chr8.3:20109750-20109769 | None:intergenic | 50.0% |
| !! | GATGATAGCGGAAATGGTGG+TGG | + | chr8.3:20110080-20110099 | MS.gene71940:intron | 50.0% |
| !! | GATTCAGGTGGCGGAATTGT+TGG | - | chr8.3:20109735-20109754 | None:intergenic | 50.0% |
| !! | GGTGATGATAGCGGAAATGG+TGG | + | chr8.3:20110077-20110096 | MS.gene71940:intron | 50.0% |
| !! | GGTGGTGATGATAGCGGAAA+TGG | + | chr8.3:20110074-20110093 | MS.gene71940:intron | 50.0% |
| !! | GGTTATGGTGTTGCTGGGAA+AGG | + | chr8.3:20109993-20110012 | MS.gene71940:CDS | 50.0% |
| !! | GTTGGTGGTGGTGATGATAG+CGG | + | chr8.3:20110068-20110087 | MS.gene71940:intron | 50.0% |
| ACAGCACGAACAGTCTCACC+AGG | - | chr8.3:20109945-20109964 | None:intergenic | 55.0% | |
| GGTGAGACTGTTCGTGCTGT+TGG | + | chr8.3:20109945-20109964 | MS.gene71940:CDS | 55.0% | |
| TCAGGTTCGGATTCAGGTGG+CGG | - | chr8.3:20109744-20109763 | None:intergenic | 55.0% | |
| ! | GACTGTTCGTGCTGTTGGAC+CGG | + | chr8.3:20109950-20109969 | MS.gene71940:CDS | 55.0% |
| ! | GCTTCAGGTTCGGATTCAGG+TGG | - | chr8.3:20109747-20109766 | None:intergenic | 55.0% |
| !! | AGAGGGAGAGAGCGTTGATG+CGG | + | chr8.3:20110183-20110202 | MS.gene71940:CDS | 55.0% |
| !! | GCGGAAATGGTGGTGGTGAT+AGG | + | chr8.3:20110087-20110106 | MS.gene71940:intron | 55.0% |
| CGGCGGCTTCTTCGTCTTTG+CGG | - | chr8.3:20109623-20109642 | None:intergenic | 60.0% | |
| !! | AGCGTTGATGCGGCACCCGA+AGG | + | chr8.3:20110193-20110212 | MS.gene71940:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 20109543 | 20110262 | 20109543 | ID=MS.gene71940 |
| chr8.3 | mRNA | 20109543 | 20110262 | 20109543 | ID=MS.gene71940.t1;Parent=MS.gene71940 |
| chr8.3 | exon | 20109543 | 20109723 | 20109543 | ID=MS.gene71940.t1.exon1;Parent=MS.gene71940.t1 |
| chr8.3 | CDS | 20109543 | 20109723 | 20109543 | ID=cds.MS.gene71940.t1;Parent=MS.gene71940.t1 |
| chr8.3 | exon | 20109820 | 20110030 | 20109820 | ID=MS.gene71940.t1.exon2;Parent=MS.gene71940.t1 |
| chr8.3 | CDS | 20109820 | 20110030 | 20109820 | ID=cds.MS.gene71940.t1;Parent=MS.gene71940.t1 |
| chr8.3 | exon | 20110124 | 20110262 | 20110124 | ID=MS.gene71940.t1.exon3;Parent=MS.gene71940.t1 |
| chr8.3 | CDS | 20110124 | 20110262 | 20110124 | ID=cds.MS.gene71940.t1;Parent=MS.gene71940.t1 |
| Gene Sequence |
| Protein sequence |
