AlfalfaGEDB | MS.gene77188

M. sativa cultivar XinJiangDaYe / MS.gene77188

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77188.t1	XP_003595828.2	97.4	154	3	1	1	153	1	154	5.00E-72	280.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77188.t1	A2Q3C1	97.4	154	3	1	1	153	1	154	3.6e-72	280.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049501	MS.gene77188	0.831228	1.88E-55	-1.69E-46
MS.gene050787	MS.gene77188	0.817625	2.99E-52	-1.69E-46
MS.gene050844	MS.gene77188	0.822581	2.19E-53	-1.69E-46
MS.gene051265	MS.gene77188	0.857516	1.55E-62	-1.69E-46
MS.gene051388	MS.gene77188	0.826245	3.01E-54	-1.69E-46
MS.gene052020	MS.gene77188	0.817027	4.08E-52	-1.69E-46
MS.gene052042	MS.gene77188	0.816446	5.51E-52	-1.69E-46
MS.gene052561	MS.gene77188	0.807965	3.91E-50	-1.69E-46
MS.gene052611	MS.gene77188	0.808684	2.74E-50	-1.69E-46
MS.gene052863	MS.gene77188	0.817273	3.59E-52	-1.69E-46
MS.gene05288	MS.gene77188	0.814386	1.58E-51	-1.69E-46
MS.gene052896	MS.gene77188	0.811683	6.19E-51	-1.69E-46
MS.gene05289	MS.gene77188	0.817855	2.66E-52	-1.69E-46
MS.gene053584	MS.gene77188	0.806824	6.82E-50	-1.69E-46
MS.gene05417	MS.gene77188	0.808319	3.28E-50	-1.69E-46
MS.gene054236	MS.gene77188	0.807426	5.08E-50	-1.69E-46
MS.gene054467	MS.gene77188	0.833307	5.75E-56	-1.69E-46
MS.gene054472	MS.gene77188	0.802198	6.29E-49	-1.69E-46
MS.gene05468	MS.gene77188	0.820265	7.52E-53	-1.69E-46
MS.gene054713	MS.gene77188	0.801468	8.88E-49	-1.69E-46
MS.gene054775	MS.gene77188	0.803208	3.89E-49	-1.69E-46
MS.gene054801	MS.gene77188	0.828357	9.40E-55	-1.69E-46
MS.gene055356	MS.gene77188	0.831623	1.50E-55	-1.69E-46
MS.gene055357	MS.gene77188	0.818012	2.45E-52	-1.69E-46
MS.gene055358	MS.gene77188	0.818769	1.65E-52	-1.69E-46
MS.gene055466	MS.gene77188	0.816617	5.04E-52	-1.69E-46
MS.gene055608	MS.gene77188	0.837001	6.72E-57	-1.69E-46
MS.gene055804	MS.gene77188	0.806334	8.65E-50	-1.69E-46
MS.gene055818	MS.gene77188	0.81216	4.88E-51	-1.69E-46
MS.gene056173	MS.gene77188	0.81785	2.66E-52	-1.69E-46
MS.gene056553	MS.gene77188	0.813216	2.86E-51	-1.69E-46
MS.gene058168	MS.gene77188	0.823041	1.71E-53	-1.69E-46
MS.gene058515	MS.gene77188	0.814713	1.34E-51	-1.69E-46
MS.gene058625	MS.gene77188	0.807425	5.09E-50	-1.69E-46
MS.gene059124	MS.gene77188	0.808805	2.59E-50	-1.69E-46
MS.gene059128	MS.gene77188	0.807375	5.21E-50	-1.69E-46
MS.gene059272	MS.gene77188	0.811773	5.92E-51	-1.69E-46
MS.gene060137	MS.gene77188	-0.814778	1.30E-51	-1.69E-46
MS.gene060167	MS.gene77188	0.802227	6.20E-49	-1.69E-46
MS.gene060316	MS.gene77188	0.80205	6.74E-49	-1.69E-46
MS.gene060322	MS.gene77188	0.812079	5.08E-51	-1.69E-46
MS.gene060781	MS.gene77188	0.800561	1.36E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77188.t1	MTR_2g062280	97.403	154	3	1	1	153	1	154	4.27e-107	302

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77188.t1	AT2G31930	33.544	158	77	7	2	151	4	141	3.62e-11	57.8

Find 26 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGTCGTTATGCTAAAGTTT+TGG	0.258762	2.4:+31594339	MS.gene77188:CDS
AGAAACAACTTCAACTTGTT+AGG	0.273115	2.4:+31594269	MS.gene77188:CDS
TCTAAGCGTGATCCTCATTT+AGG	0.318366	2.4:+31594376	MS.gene77188:CDS
GGTGTTTCAACCTTTGAGAT+TGG	0.400760	2.4:-31594064	None:intergenic
TAAGCTGATAACTTGAAAAC+AGG	0.423889	2.4:-31594415	None:intergenic
AGCTTGAAACAATTGATTGT+AGG	0.428759	2.4:+31594157	MS.gene77188:CDS
GAAACAACTTCAACTTGTTA+GGG	0.446869	2.4:+31594270	MS.gene77188:CDS
TCATTACCTTCTTTCACCTC+AGG	0.450380	2.4:-31594115	None:intergenic
GATCCTCATTTAGGTGATGC+TGG	0.461176	2.4:+31594385	MS.gene77188:CDS
CAACAAAAGGACAATCTTCA+AGG	0.467431	2.4:+31594295	MS.gene77188:CDS
TTGAAGTTGTTTCTCTTCCT+CGG	0.467806	2.4:-31594260	None:intergenic
ACTTGTTAGGGTGCAACAAA+AGG	0.473235	2.4:+31594282	MS.gene77188:CDS
AGGTTTGAAGTTTAAGAAGT+TGG	0.481500	2.4:+31594315	MS.gene77188:CDS
AAGAAGTGTAGTAGTGATGA+AGG	0.488228	2.4:+31594199	MS.gene77188:CDS
TTCCCAGCATCACCTAAATG+AGG	0.511600	2.4:-31594388	None:intergenic
ACCATTCCTGAGGTGAAAGA+AGG	0.519436	2.4:+31594109	MS.gene77188:CDS
GTTTCAAGTGAAACATCCTT+AGG	0.528693	2.4:-31594085	None:intergenic
TTGACATAGTTATGTCATAG+TGG	0.535617	2.4:-31594003	None:intergenic
ATCCTCATTTAGGTGATGCT+GGG	0.548085	2.4:+31594386	MS.gene77188:CDS
CTCAAAGGTTGAAACACCTA+AGG	0.551532	2.4:+31594069	MS.gene77188:CDS
ACCTTCTTTCACCTCAGGAA+TGG	0.551885	2.4:-31594110	None:intergenic
AGTGATGAAGGTGAAAGCAA+AGG	0.564280	2.4:+31594211	MS.gene77188:CDS
GTCATAGTGGCGTTTCGTCG+AGG	0.574257	2.4:-31593990	None:intergenic
AGCAAAGGAAGAAACTCGCT+CGG	0.605530	2.4:+31594226	MS.gene77188:CDS
ACTTGAAACTACCATTCCTG+AGG	0.625626	2.4:+31594099	MS.gene77188:CDS
TAACTATGTCAAGAAGAACA+AGG	0.716307	2.4:+31594014	MS.gene77188:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGCTTGAAACAATTGATTGT+AGG	+	chr2.4:31594157-31594176	MS.gene77188:CDS	30.0%
	CAATGAAAAACCAATCTCAA+AGG	+	chr2.4:31594054-31594073	MS.gene77188:CDS	30.0%
	TAACTATGTCAAGAAGAACA+AGG	+	chr2.4:31594014-31594033	MS.gene77188:CDS	30.0%
	TAAGCTGATAACTTGAAAAC+AGG	-	chr2.4:31594418-31594437	None:intergenic	30.0%
	TTGACATAGTTATGTCATAG+TGG	-	chr2.4:31594006-31594025	None:intergenic	30.0%
!	AGAAACAACTTCAACTTGTT+AGG	+	chr2.4:31594269-31594288	MS.gene77188:CDS	30.0%
!	AGGTTTGAAGTTTAAGAAGT+TGG	+	chr2.4:31594315-31594334	MS.gene77188:CDS	30.0%
!!	GAAACAACTTCAACTTGTTA+GGG	+	chr2.4:31594270-31594289	MS.gene77188:CDS	30.0%
!!!	CTTTGAGATTGGTTTTTCAT+TGG	-	chr2.4:31594056-31594075	None:intergenic	30.0%
	CAACAAAAGGACAATCTTCA+AGG	+	chr2.4:31594295-31594314	MS.gene77188:CDS	35.0%
!	AAGAAGTGTAGTAGTGATGA+AGG	+	chr2.4:31594199-31594218	MS.gene77188:CDS	35.0%
!	CGTCGTTATGCTAAAGTTTT+GGG	+	chr2.4:31594340-31594359	MS.gene77188:CDS	35.0%
!	GTCGTTATGCTAAAGTTTTG+GGG	+	chr2.4:31594341-31594360	MS.gene77188:CDS	35.0%
!	GTTTCAAGTGAAACATCCTT+AGG	-	chr2.4:31594088-31594107	None:intergenic	35.0%
!	TCGTCGTTATGCTAAAGTTT+TGG	+	chr2.4:31594339-31594358	MS.gene77188:CDS	35.0%
!!	TTGAAGTTGTTTCTCTTCCT+CGG	-	chr2.4:31594263-31594282	None:intergenic	35.0%
	ACTTGAAACTACCATTCCTG+AGG	+	chr2.4:31594099-31594118	MS.gene77188:CDS	40.0%
	AGTGATGAAGGTGAAAGCAA+AGG	+	chr2.4:31594211-31594230	MS.gene77188:CDS	40.0%
	ATCCTCATTTAGGTGATGCT+GGG	+	chr2.4:31594386-31594405	MS.gene77188:CDS	40.0%
	CTCAAAGGTTGAAACACCTA+AGG	+	chr2.4:31594069-31594088	MS.gene77188:CDS	40.0%
	TCATTACCTTCTTTCACCTC+AGG	-	chr2.4:31594118-31594137	None:intergenic	40.0%
	TCTAAGCGTGATCCTCATTT+AGG	+	chr2.4:31594376-31594395	MS.gene77188:CDS	40.0%
!	ACTTGTTAGGGTGCAACAAA+AGG	+	chr2.4:31594282-31594301	MS.gene77188:CDS	40.0%
!!	GGTGTTTCAACCTTTGAGAT+TGG	-	chr2.4:31594067-31594086	None:intergenic	40.0%
	ACCATTCCTGAGGTGAAAGA+AGG	+	chr2.4:31594109-31594128	MS.gene77188:CDS	45.0%
	ACCTTCTTTCACCTCAGGAA+TGG	-	chr2.4:31594113-31594132	None:intergenic	45.0%
	AGCAAAGGAAGAAACTCGCT+CGG	+	chr2.4:31594226-31594245	MS.gene77188:CDS	45.0%
	GATCCTCATTTAGGTGATGC+TGG	+	chr2.4:31594385-31594404	MS.gene77188:CDS	45.0%
	TTCCCAGCATCACCTAAATG+AGG	-	chr2.4:31594391-31594410	None:intergenic	45.0%
!!	GCTCGGTGAACATTTTACCG+AGG	+	chr2.4:31594243-31594262	MS.gene77188:CDS	50.0%
	GTCATAGTGGCGTTTCGTCG+AGG	-	chr2.4:31593993-31594012	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	31593977	31594438	31593977	ID=MS.gene77188
chr2.4	mRNA	31593977	31594438	31593977	ID=MS.gene77188.t1;Parent=MS.gene77188
chr2.4	exon	31593977	31594438	31593977	ID=MS.gene77188.t1.exon1;Parent=MS.gene77188.t1
chr2.4	CDS	31593977	31594438	31593977	ID=cds.MS.gene77188.t1;Parent=MS.gene77188.t1

Gene Sequence

>MS.gene77188

ATGGAGGTTCAATCCTCGACGAAACGCCACTATGACATAACTATGTCAAGAAGAACAAGGAAACAACAATCATTAGCCAATGAAAAACCAATCTCAAAGGTTGAAACACCTAAGGATGTTTCACTTGAAACTACCATTCCTGAGGTGAAAGAAGGTAATGAGAATAATATTGATCACAAAAGCTTGAAACAATTGATTGTAGGAGAAGAGAAAGAATCAACAAAGAAGTGTAGTAGTGATGAAGGTGAAAGCAAAGGAAGAAACTCGCTCGGTGAACATTTTACCGAGGAAGAGAAACAACTTCAACTTGTTAGGGTGCAACAAAAGGACAATCTTCAAGGTTTGAAGTTTAAGAAGTTGGTTCGTCGTTATGCTAAAGTTTTGGGGCATATGATGAAGTCTAAGCGTGATCCTCATTTAGGTGATGCTGGGAAAAAACCTGTTTTCAAGTTATCAGCTTAG

Protein sequence

>MS.gene77188.t1

MEVQSSTKRHYDITMSRRTRKQQSLANEKPISKVETPKDVSLETTIPEVKEGNENNIDHKSLKQLIVGEEKESTKKCSSDEGESKGRNSLGEHFTEEEKQLQLVRVQQKDNLQGLKFKKLVRRYAKVLGHMMKSKRDPHLGDAGKKPVFKLSA