AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81121


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81121.t1 MTR_2g061450 93.309 269 18 0 1 269 164 432 0.0 513
MS.gene81121.t1 MTR_2g060620 87.387 111 12 1 139 249 61 169 1.63e-62 195
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81121.t1 AT1G74120 57.993 269 113 0 1 269 177 445 1.81e-113 334
MS.gene81121.t1 AT5G54180 30.270 185 124 4 10 191 290 472 1.79e-14 73.2
MS.gene81121.t1 AT1G62010 32.787 122 79 3 101 220 268 388 2.86e-13 69.7
MS.gene81121.t1 AT1G62120 29.167 120 84 1 102 220 291 410 4.67e-11 63.2
MS.gene81121.t1 AT1G62085 25.414 181 127 4 46 220 266 444 6.67e-11 62.4
MS.gene81121.t1 AT1G62085 25.414 181 127 4 46 220 266 444 6.67e-11 62.4
MS.gene81121.t1 AT5G23930 29.091 110 74 1 120 225 336 445 6.82e-11 62.4

Find 63 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATTGTTTCCTAGGGTTT+TGG 0.194253 2.3:+32499972 MS.gene81121:CDS
ATTCATGAGCTGAGGGGTTT+GGG 0.198657 2.3:+32500027 MS.gene81121:CDS
CATGTTCATCACATCTTTAC+TGG 0.212061 2.3:-32500652 None:intergenic
GATTCATGAGCTGAGGGGTT+TGG 0.264040 2.3:+32500026 MS.gene81121:CDS
CAACACTATATTTCATCCTT+TGG 0.303808 2.3:-32500347 None:intergenic
GAGGTTGATTGGTTATGATT+TGG 0.308965 2.3:+32500293 MS.gene81121:CDS
AGGTCAGGGCGGAGGTTGTT+AGG 0.309694 2.3:+32500064 MS.gene81121:CDS
TTGAAAGGAAAAGGTGCAAT+TGG 0.315697 2.3:+32500450 MS.gene81121:CDS
AAATTCACACCCAAGTATTC+AGG 0.317076 2.3:-32500387 None:intergenic
GATGAAATTGATAGAGTTGT+TGG 0.328831 2.3:+32499952 MS.gene81121:CDS
GAGTTGTTGGATTGTTTCCT+AGG 0.358743 2.3:+32499965 MS.gene81121:CDS
GATGAACATGAAGGCTTTCA+TGG 0.369314 2.3:+32500665 MS.gene81121:CDS
GGTGCAATTGGGTTTGAAGT+TGG 0.399622 2.3:+32500462 MS.gene81121:CDS
ACTCTATCAATTTCATCCTT+AGG 0.408293 2.3:-32499946 None:intergenic
TGGGATTTGGTATTGAGGAT+AGG 0.409253 2.3:+32499992 MS.gene81121:CDS
GAAAGCATCCCGTTGGACTT+TGG 0.413704 2.3:+32500598 MS.gene81121:CDS
TCACATCTTTACTGGTTTGA+GGG 0.416518 2.3:-32500644 None:intergenic
AATTCGTAGGGATGCTTTGA+AGG 0.418729 2.3:+32500254 MS.gene81121:CDS
TGGAAGGAACCGAGGTTGAT+TGG 0.420423 2.3:+32500282 MS.gene81121:CDS
ATCACATCTTTACTGGTTTG+AGG 0.423102 2.3:-32500645 None:intergenic
TGAAAGGAAAAGGTGCAATT+GGG 0.424464 2.3:+32500451 MS.gene81121:CDS
ATCTCCATTCCTAAAATCCT+TGG 0.428501 2.3:-32500090 None:intergenic
TGTTGCAGAGTTTGAAATGC+AGG 0.431814 2.3:+32500139 MS.gene81121:CDS
GCATGAAGTTCCTGAATACT+TGG 0.443861 2.3:+32500377 MS.gene81121:CDS
CCCCTCAGCTCATGAATCAA+TGG 0.448660 2.3:-32500021 None:intergenic
CAGCCGAGAGGTCAGGGCGG+AGG 0.450679 2.3:+32500056 MS.gene81121:CDS
TACTCAATCACATTGTATCT+AGG 0.456654 2.3:-32500426 None:intergenic
GCGGTCACGGTTTAATTCGT+AGG 0.464329 2.3:+32500241 MS.gene81121:CDS
GTTGATTGGTTATGATTTGG+AGG 0.468999 2.3:+32500296 MS.gene81121:CDS
TTATAAAATCTAAGCCTAGT+TGG 0.470603 2.3:-32500504 None:intergenic
CTCCGCCCTGACCTCTCGGC+TGG 0.473380 2.3:-32500055 None:intergenic
ATTGAGTATTTGAAAGGAAA+AGG 0.475963 2.3:+32500441 MS.gene81121:CDS
GGTCAGGGCGGAGGTTGTTA+GGG 0.481020 2.3:+32500065 MS.gene81121:CDS
AACATCTTCCAAAGTCCAAC+GGG 0.486105 2.3:-32500606 None:intergenic
GTGGATTGTTTGTGCGGTCA+CGG 0.490001 2.3:+32500228 MS.gene81121:CDS
TGTGGAATTTGGGATTCCTA+AGG 0.495755 2.3:+32499930 MS.gene81121:CDS
AGTTGTTGGATTGTTTCCTA+GGG 0.504801 2.3:+32499966 MS.gene81121:CDS
AATGTGATTGAGTATTTGAA+AGG 0.505696 2.3:+32500435 MS.gene81121:CDS
TTTGAGGGAACTTCGGTGGT+TGG 0.508881 2.3:-32500629 None:intergenic
TCATGGACTCGTTGGTGTAG+TGG 0.510176 2.3:+32500682 MS.gene81121:CDS
CGGTCACGGTTTAATTCGTA+GGG 0.517633 2.3:+32500242 MS.gene81121:CDS
TTCATGAGCTGAGGGGTTTG+GGG 0.523718 2.3:+32500028 MS.gene81121:CDS
GCCATTGATTCATGAGCTGA+GGG 0.528936 2.3:+32500020 MS.gene81121:CDS
TTACTGGTTTGAGGGAACTT+CGG 0.536810 2.3:-32500636 None:intergenic
CGGAGGTTGTTAGGGATCCA+AGG 0.538817 2.3:+32500073 MS.gene81121:CDS
CAACCTCCGCCCTGACCTCT+CGG 0.563699 2.3:-32500059 None:intergenic
ATACCAAATCCCAAAACCCT+AGG 0.565652 2.3:-32499982 None:intergenic
GAAGGCTTTCATGGACTCGT+TGG 0.570328 2.3:+32500674 MS.gene81121:CDS
CTGGTTTGAGGGAACTTCGG+TGG 0.572836 2.3:-32500633 None:intergenic
TGCTTTGAAGGTGTTGTGGA+AGG 0.580553 2.3:+32500266 MS.gene81121:CDS
CATGAAGTTCCTGAATACTT+GGG 0.593187 2.3:+32500378 MS.gene81121:CDS
AGCCATTGATTCATGAGCTG+AGG 0.597553 2.3:+32500019 MS.gene81121:CDS
AAGACATTATCAAACCAACT+AGG 0.602970 2.3:+32500490 MS.gene81121:CDS
GTTAAAAGAAAGCATCCCGT+TGG 0.604141 2.3:+32500591 MS.gene81121:CDS
TAAAGATGTGATGAACATGA+AGG 0.609470 2.3:+32500656 MS.gene81121:CDS
GGGATGCTTTGAAGGTGTTG+TGG 0.613256 2.3:+32500262 MS.gene81121:CDS
GTTGCAGAGTTTGAAATGCA+GGG 0.619561 2.3:+32500140 MS.gene81121:CDS
GAACATCTTCCAAAGTCCAA+CGG 0.624427 2.3:-32500607 None:intergenic
CTGAGAGTGGATTGTTTGTG+CGG 0.636549 2.3:+32500222 MS.gene81121:CDS
AAATCATAACCAATCAACCT+CGG 0.663302 2.3:-32500291 None:intergenic
CCATTGATTCATGAGCTGAG+GGG 0.690477 2.3:+32500021 MS.gene81121:CDS
TTCCAGCCGAGAGGTCAGGG+CGG 0.702811 2.3:+32500053 MS.gene81121:CDS
AGGTGTTGTGGAAGGAACCG+AGG 0.744040 2.3:+32500274 MS.gene81121:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAAAGAGAAGATTTTTG+GGG + chr2.3:32500167-32500186 MS.gene81121:CDS 20.0%
!!! CAATTAAAGAGAAGATTTTT+GGG + chr2.3:32500166-32500185 MS.gene81121:CDS 20.0%
!!! CATATATTTTTTCACATTCA+GGG - chr2.3:32500548-32500567 None:intergenic 20.0%
! AATGTGATTGAGTATTTGAA+AGG + chr2.3:32500435-32500454 MS.gene81121:CDS 25.0%
! ATTGAGTATTTGAAAGGAAA+AGG + chr2.3:32500441-32500460 MS.gene81121:CDS 25.0%
! CCTGAATGTGAAAAAATATA+TGG + chr2.3:32500546-32500565 MS.gene81121:CDS 25.0%
! TTATAAAATCTAAGCCTAGT+TGG - chr2.3:32500507-32500526 None:intergenic 25.0%
!! AGATTGAGTTTTTAATCCAA+AGG + chr2.3:32500331-32500350 MS.gene81121:CDS 25.0%
!! GCAATTAAAGAGAAGATTTT+TGG + chr2.3:32500165-32500184 MS.gene81121:CDS 25.0%
!! TTGATTTTCTTGTGGAATTT+GGG + chr2.3:32499920-32499939 MS.gene81121:CDS 25.0%
!!! ATTGATTTTCTTGTGGAATT+TGG + chr2.3:32499919-32499938 MS.gene81121:CDS 25.0%
!!! CCATATATTTTTTCACATTC+AGG - chr2.3:32500549-32500568 None:intergenic 25.0%
AAATCATAACCAATCAACCT+CGG - chr2.3:32500294-32500313 None:intergenic 30.0%
AAGACATTATCAAACCAACT+AGG + chr2.3:32500490-32500509 MS.gene81121:CDS 30.0%
ACTCTATCAATTTCATCCTT+AGG - chr2.3:32499949-32499968 None:intergenic 30.0%
CAACACTATATTTCATCCTT+TGG - chr2.3:32500350-32500369 None:intergenic 30.0%
GATGAAATTGATAGAGTTGT+TGG + chr2.3:32499952-32499971 MS.gene81121:CDS 30.0%
TAAAGATGTGATGAACATGA+AGG + chr2.3:32500656-32500675 MS.gene81121:CDS 30.0%
TACTCAATCACATTGTATCT+AGG - chr2.3:32500429-32500448 None:intergenic 30.0%
! ATATGGCAGATTTTCAACAA+AGG + chr2.3:32500563-32500582 MS.gene81121:CDS 30.0%
!!! ATTTTTTCACATTCAGGGTA+AGG - chr2.3:32500543-32500562 None:intergenic 30.0%
!!! TAGTGTCATTGATTTTCTTG+TGG + chr2.3:32499912-32499931 MS.gene81121:CDS 30.0%
AAATTCACACCCAAGTATTC+AGG - chr2.3:32500390-32500409 None:intergenic 35.0%
AGTTGTTGGATTGTTTCCTA+GGG + chr2.3:32499966-32499985 MS.gene81121:CDS 35.0%
ATCTCCATTCCTAAAATCCT+TGG - chr2.3:32500093-32500112 None:intergenic 35.0%
CATGAAGTTCCTGAATACTT+GGG + chr2.3:32500378-32500397 MS.gene81121:CDS 35.0%
CATGTTCATCACATCTTTAC+TGG - chr2.3:32500655-32500674 None:intergenic 35.0%
! GAGGTTGATTGGTTATGATT+TGG + chr2.3:32500293-32500312 MS.gene81121:CDS 35.0%
! GTTGATTGGTTATGATTTGG+AGG + chr2.3:32500296-32500315 MS.gene81121:CDS 35.0%
! TGAAAGGAAAAGGTGCAATT+GGG + chr2.3:32500451-32500470 MS.gene81121:CDS 35.0%
! TTGAAAGGAAAAGGTGCAAT+TGG + chr2.3:32500450-32500469 MS.gene81121:CDS 35.0%
!! AAAGAGAAGATTTTTGGGGA+GGG + chr2.3:32500171-32500190 MS.gene81121:CDS 35.0%
!! AGGATTTTAGGAATGGAGAT+TGG + chr2.3:32500093-32500112 MS.gene81121:CDS 35.0%
!! ATCACATCTTTACTGGTTTG+AGG - chr2.3:32500648-32500667 None:intergenic 35.0%
!! GATTTTAGGAATGGAGATTG+GGG + chr2.3:32500095-32500114 MS.gene81121:CDS 35.0%
!! GGATTTTAGGAATGGAGATT+GGG + chr2.3:32500094-32500113 MS.gene81121:CDS 35.0%
!! TAAAGAGAAGATTTTTGGGG+AGG + chr2.3:32500170-32500189 MS.gene81121:CDS 35.0%
!! TCACATCTTTACTGGTTTGA+GGG - chr2.3:32500647-32500666 None:intergenic 35.0%
AACATCTTCCAAAGTCCAAC+GGG - chr2.3:32500609-32500628 None:intergenic 40.0%
AATTCGTAGGGATGCTTTGA+AGG + chr2.3:32500254-32500273 MS.gene81121:CDS 40.0%
ATACCAAATCCCAAAACCCT+AGG - chr2.3:32499985-32500004 None:intergenic 40.0%
GAACATCTTCCAAAGTCCAA+CGG - chr2.3:32500610-32500629 None:intergenic 40.0%
GAGTTGTTGGATTGTTTCCT+AGG + chr2.3:32499965-32499984 MS.gene81121:CDS 40.0%
GATGAACATGAAGGCTTTCA+TGG + chr2.3:32500665-32500684 MS.gene81121:CDS 40.0%
GCATGAAGTTCCTGAATACT+TGG + chr2.3:32500377-32500396 MS.gene81121:CDS 40.0%
! GGATTGTTTCCTAGGGTTTT+GGG + chr2.3:32499973-32499992 MS.gene81121:CDS 40.0%
! GTTAGGGATCCAAGGATTTT+AGG + chr2.3:32500081-32500100 MS.gene81121:CDS 40.0%
! GTTGCAGAGTTTGAAATGCA+GGG + chr2.3:32500140-32500159 MS.gene81121:CDS 40.0%
! TGGAGATTGGGGAATTTTCA+CGG + chr2.3:32500106-32500125 MS.gene81121:CDS 40.0%
! TGGATTGTTTCCTAGGGTTT+TGG + chr2.3:32499972-32499991 MS.gene81121:CDS 40.0%
! TGGGATTTGGTATTGAGGAT+AGG + chr2.3:32499992-32500011 MS.gene81121:CDS 40.0%
! TGTGGAATTTGGGATTCCTA+AGG + chr2.3:32499930-32499949 MS.gene81121:CDS 40.0%
! TGTTGCAGAGTTTGAAATGC+AGG + chr2.3:32500139-32500158 MS.gene81121:CDS 40.0%
!! GGATCCAAGGATTTTAGGAA+TGG + chr2.3:32500086-32500105 MS.gene81121:CDS 40.0%
!! GTTAAAAGAAAGCATCCCGT+TGG + chr2.3:32500591-32500610 MS.gene81121:CDS 40.0%
!! TTACTGGTTTGAGGGAACTT+CGG - chr2.3:32500639-32500658 None:intergenic 40.0%
!!! GGTTTTGGGATTTGGTATTG+AGG + chr2.3:32499987-32500006 MS.gene81121:CDS 40.0%
!!! TTTCCTAGGGTTTTGGGATT+TGG + chr2.3:32499979-32499998 MS.gene81121:CDS 40.0%
!!! TTTTGAAGTGAAGCTGAGAG+TGG + chr2.3:32500209-32500228 MS.gene81121:CDS 40.0%
ATTCATGAGCTGAGGGGTTT+GGG + chr2.3:32500027-32500046 MS.gene81121:CDS 45.0%
CGGTCACGGTTTAATTCGTA+GGG + chr2.3:32500242-32500261 MS.gene81121:CDS 45.0%
! GGTGCAATTGGGTTTGAAGT+TGG + chr2.3:32500462-32500481 MS.gene81121:CDS 45.0%
!! AGCCATTGATTCATGAGCTG+AGG + chr2.3:32500019-32500038 MS.gene81121:CDS 45.0%
!! CCATTGATTCATGAGCTGAG+GGG + chr2.3:32500021-32500040 MS.gene81121:CDS 45.0%
!! CTGAGAGTGGATTGTTTGTG+CGG + chr2.3:32500222-32500241 MS.gene81121:CDS 45.0%
!! GAAGATTTTTGGGGAGGGTT+TGG + chr2.3:32500176-32500195 MS.gene81121:CDS 45.0%
!! GCCATTGATTCATGAGCTGA+GGG + chr2.3:32500020-32500039 MS.gene81121:CDS 45.0%
!! TGCTTTGAAGGTGTTGTGGA+AGG + chr2.3:32500266-32500285 MS.gene81121:CDS 45.0%
!! TTTTGGGGAGGGTTTGGTTA+GGG + chr2.3:32500182-32500201 MS.gene81121:CDS 45.0%
!! TTTTTGGGGAGGGTTTGGTT+AGG + chr2.3:32500181-32500200 MS.gene81121:CDS 45.0%
CCCCTCAGCTCATGAATCAA+TGG - chr2.3:32500024-32500043 None:intergenic 50.0%
GAAGGCTTTCATGGACTCGT+TGG + chr2.3:32500674-32500693 MS.gene81121:CDS 50.0%
GATTCATGAGCTGAGGGGTT+TGG + chr2.3:32500026-32500045 MS.gene81121:CDS 50.0%
GCGGTCACGGTTTAATTCGT+AGG + chr2.3:32500241-32500260 MS.gene81121:CDS 50.0%
TTCATGAGCTGAGGGGTTTG+GGG + chr2.3:32500028-32500047 MS.gene81121:CDS 50.0%
TTTGAGGGAACTTCGGTGGT+TGG - chr2.3:32500632-32500651 None:intergenic 50.0%
! GTGGATTGTTTGTGCGGTCA+CGG + chr2.3:32500228-32500247 MS.gene81121:CDS 50.0%
! TGGAAGGAACCGAGGTTGAT+TGG + chr2.3:32500282-32500301 MS.gene81121:CDS 50.0%
!! GAAAGCATCCCGTTGGACTT+TGG + chr2.3:32500598-32500617 MS.gene81121:CDS 50.0%
!! GGGATGCTTTGAAGGTGTTG+TGG + chr2.3:32500262-32500281 MS.gene81121:CDS 50.0%
CGGAGGTTGTTAGGGATCCA+AGG + chr2.3:32500073-32500092 MS.gene81121:CDS 55.0%
! AGGTGTTGTGGAAGGAACCG+AGG + chr2.3:32500274-32500293 MS.gene81121:CDS 55.0%
! CTGGTTTGAGGGAACTTCGG+TGG - chr2.3:32500636-32500655 None:intergenic 55.0%
! GTTTTCCAGCCGAGAGGTCA+GGG + chr2.3:32500050-32500069 MS.gene81121:CDS 55.0%
!! TTTGGGGTTTTCCAGCCGAG+AGG + chr2.3:32500044-32500063 MS.gene81121:CDS 55.0%
AGGTCAGGGCGGAGGTTGTT+AGG + chr2.3:32500064-32500083 MS.gene81121:CDS 60.0%
GGTCAGGGCGGAGGTTGTTA+GGG + chr2.3:32500065-32500084 MS.gene81121:CDS 60.0%
! GGTTTTCCAGCCGAGAGGTC+AGG + chr2.3:32500049-32500068 MS.gene81121:CDS 60.0%
CAACCTCCGCCCTGACCTCT+CGG - chr2.3:32500062-32500081 None:intergenic 65.0%
TTCCAGCCGAGAGGTCAGGG+CGG + chr2.3:32500053-32500072 MS.gene81121:CDS 65.0%
CAGCCGAGAGGTCAGGGCGG+AGG + chr2.3:32500056-32500075 MS.gene81121:CDS 75.0%
CTCCGCCCTGACCTCTCGGC+TGG - chr2.3:32500058-32500077 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 32499892 32500701 32499892 ID=MS.gene81121
chr2.3 mRNA 32499892 32500701 32499892 ID=MS.gene81121.t1;Parent=MS.gene81121
chr2.3 exon 32499892 32500701 32499892 ID=MS.gene81121.t1.exon1;Parent=MS.gene81121.t1
chr2.3 CDS 32499892 32500701 32499892 ID=cds.MS.gene81121.t1;Parent=MS.gene81121.t1
Gene Sequence

>MS.gene81121

ATGGGGGAAACTCAAATTGTTAGTGTCATTGATTTTCTTGTGGAATTTGGGATTCCTAAGGATGAAATTGATAGAGTTGTTGGATTGTTTCCTAGGGTTTTGGGATTTGGTATTGAGGATAGGCTGAAGCCATTGATTCATGAGCTGAGGGGTTTGGGGTTTTCCAGCCGAGAGGTCAGGGCGGAGGTTGTTAGGGATCCAAGGATTTTAGGAATGGAGATTGGGGAATTTTCACGGTGTTTGAAGTTGTTGCAGAGTTTGAAATGCAGGGATGCAATTAAAGAGAAGATTTTTGGGGAGGGTTTGGTTAGGGCTTGTTTTGAAGTGAAGCTGAGAGTGGATTGTTTGTGCGGTCACGGTTTAATTCGTAGGGATGCTTTGAAGGTGTTGTGGAAGGAACCGAGGTTGATTGGTTATGATTTGGAGGATATTGAGAAGAAGATTGAGTTTTTAATCCAAAGGATGAAATATAGTGTTGATTGTTTGCATGAAGTTCCTGAATACTTGGGTGTGAATTTTGAAAAACAGATTGTTCCTAGATACAATGTGATTGAGTATTTGAAAGGAAAAGGTGCAATTGGGTTTGAAGTTGGATTGAAAGACATTATCAAACCAACTAGGCTTAGATTTTATAATCTTTATGTCAAGCCTTACCCTGAATGTGAAAAAATATATGGCAGATTTTCAACAAAGGTTGAAGTTAAAAGAAAGCATCCCGTTGGACTTTGGAAGATGTTCCAACCACCGAAGTTCCCTCAAACCAGTAAAGATGTGATGAACATGAAGGCTTTCATGGACTCGTTGGTGTAG

Protein sequence

>MS.gene81121.t1

MGETQIVSVIDFLVEFGIPKDEIDRVVGLFPRVLGFGIEDRLKPLIHELRGLGFSSREVRAEVVRDPRILGMEIGEFSRCLKLLQSLKCRDAIKEKIFGEGLVRACFEVKLRVDCLCGHGLIRRDALKVLWKEPRLIGYDLEDIEKKIEFLIQRMKYSVDCLHEVPEYLGVNFEKQIVPRYNVIEYLKGKGAIGFEVGLKDIIKPTRLRFYNLYVKPYPECEKIYGRFSTKVEVKRKHPVGLWKMFQPPKFPQTSKDVMNMKAFMDSLV