Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83218.t1 | XP_013448778.1 | 100 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 2.70E-37 | 164.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83218.t1 | Q9FKC0 | 93.8 | 81 | 5 | 0 | 1 | 81 | 1 | 81 | 3.6e-38 | 158.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83218.t1 | B7FMG6 | 100.0 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 2.0e-37 | 164.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049341 | MS.gene83218 | 0.81606 | 6.72E-52 | -1.69E-46 |
| MS.gene049580 | MS.gene83218 | 0.807454 | 5.02E-50 | -1.69E-46 |
| MS.gene050277 | MS.gene83218 | 0.851736 | 7.31E-61 | -1.69E-46 |
| MS.gene050807 | MS.gene83218 | 0.832791 | 7.73E-56 | -1.69E-46 |
| MS.gene050838 | MS.gene83218 | 0.851103 | 1.10E-60 | -1.69E-46 |
| MS.gene051233 | MS.gene83218 | 0.800728 | 1.26E-48 | -1.69E-46 |
| MS.gene051328 | MS.gene83218 | 0.852856 | 3.51E-61 | -1.69E-46 |
| MS.gene051330 | MS.gene83218 | 0.83529 | 1.83E-56 | -1.69E-46 |
| MS.gene051343 | MS.gene83218 | 0.818968 | 1.49E-52 | -1.69E-46 |
| MS.gene05134 | MS.gene83218 | 0.819692 | 1.02E-52 | -1.69E-46 |
| MS.gene051687 | MS.gene83218 | 0.80256 | 5.29E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene83218 | 0.803507 | 3.37E-49 | -1.69E-46 |
| MS.gene051746 | MS.gene83218 | 0.823331 | 1.47E-53 | -1.69E-46 |
| MS.gene051748 | MS.gene83218 | 0.821493 | 3.92E-53 | -1.69E-46 |
| MS.gene051850 | MS.gene83218 | 0.82733 | 1.66E-54 | -1.69E-46 |
| MS.gene051903 | MS.gene83218 | 0.848251 | 6.90E-60 | -1.69E-46 |
| MS.gene052133 | MS.gene83218 | 0.831311 | 1.79E-55 | -1.69E-46 |
| MS.gene052335 | MS.gene83218 | 0.804555 | 2.04E-49 | -1.69E-46 |
| MS.gene052947 | MS.gene83218 | 0.888583 | 5.19E-73 | -1.69E-46 |
| MS.gene053581 | MS.gene83218 | 0.806676 | 7.33E-50 | -1.69E-46 |
| MS.gene055424 | MS.gene83218 | 0.831794 | 1.36E-55 | -1.69E-46 |
| MS.gene055468 | MS.gene83218 | 0.803554 | 3.30E-49 | -1.69E-46 |
| MS.gene055580 | MS.gene83218 | -0.802556 | 5.30E-49 | -1.69E-46 |
| MS.gene055808 | MS.gene83218 | 0.832551 | 8.86E-56 | -1.69E-46 |
| MS.gene056043 | MS.gene83218 | -0.809774 | 1.60E-50 | -1.69E-46 |
| MS.gene056075 | MS.gene83218 | 0.804941 | 1.70E-49 | -1.69E-46 |
| MS.gene056091 | MS.gene83218 | 0.809231 | 2.10E-50 | -1.69E-46 |
| MS.gene056580 | MS.gene83218 | 0.816627 | 5.02E-52 | -1.69E-46 |
| MS.gene056631 | MS.gene83218 | 0.833739 | 4.49E-56 | -1.69E-46 |
| MS.gene056760 | MS.gene83218 | 0.824172 | 9.32E-54 | -1.69E-46 |
| MS.gene057057 | MS.gene83218 | 0.823038 | 1.72E-53 | -1.69E-46 |
| MS.gene057094 | MS.gene83218 | 0.843679 | 1.21E-58 | -1.69E-46 |
| MS.gene057234 | MS.gene83218 | 0.83469 | 2.59E-56 | -1.69E-46 |
| MS.gene057705 | MS.gene83218 | 0.841387 | 4.90E-58 | -1.69E-46 |
| MS.gene05781 | MS.gene83218 | 0.810537 | 1.10E-50 | -1.69E-46 |
| MS.gene058054 | MS.gene83218 | 0.828176 | 1.04E-54 | -1.69E-46 |
| MS.gene058055 | MS.gene83218 | 0.839633 | 1.41E-57 | -1.69E-46 |
| MS.gene058074 | MS.gene83218 | 0.801294 | 9.64E-49 | -1.69E-46 |
| MS.gene058238 | MS.gene83218 | 0.837386 | 5.36E-57 | -1.69E-46 |
| MS.gene058852 | MS.gene83218 | 0.806544 | 7.81E-50 | -1.69E-46 |
| MS.gene058906 | MS.gene83218 | 0.818894 | 1.54E-52 | -1.69E-46 |
| MS.gene059146 | MS.gene83218 | 0.801532 | 8.61E-49 | -1.69E-46 |
| MS.gene059171 | MS.gene83218 | 0.808822 | 2.56E-50 | -1.69E-46 |
| MS.gene05978 | MS.gene83218 | 0.801394 | 9.19E-49 | -1.69E-46 |
| MS.gene06001 | MS.gene83218 | 0.879397 | 1.29E-69 | -1.69E-46 |
| MS.gene060429 | MS.gene83218 | 0.841615 | 4.27E-58 | -1.69E-46 |
| MS.gene060587 | MS.gene83218 | 0.816577 | 5.15E-52 | -1.69E-46 |
| MS.gene060917 | MS.gene83218 | 0.82961 | 4.67E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83218.t1 | MTR_7g057770 | 100.000 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 5.20e-55 | 169 |
| MS.gene83218.t1 | MTR_1g023590 | 100.000 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 1.01e-54 | 168 |
| MS.gene83218.t1 | MTR_5g083790 | 96.296 | 81 | 3 | 0 | 1 | 81 | 1 | 81 | 1.14e-52 | 163 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83218.t1 | AT5G48760 | 93.827 | 81 | 5 | 0 | 1 | 81 | 1 | 81 | 2.08e-52 | 162 |
| MS.gene83218.t1 | AT5G48760 | 93.827 | 81 | 5 | 0 | 1 | 81 | 1 | 81 | 2.08e-52 | 162 |
| MS.gene83218.t1 | AT3G07110 | 91.358 | 81 | 7 | 0 | 1 | 81 | 1 | 81 | 1.08e-51 | 160 |
| MS.gene83218.t1 | AT3G07110 | 91.358 | 81 | 7 | 0 | 1 | 81 | 1 | 81 | 1.26e-51 | 160 |
| MS.gene83218.t1 | AT3G24830 | 91.358 | 81 | 7 | 0 | 1 | 81 | 1 | 81 | 7.39e-51 | 158 |
| MS.gene83218.t1 | AT4G13170 | 91.358 | 81 | 7 | 0 | 1 | 81 | 1 | 81 | 2.43e-50 | 157 |
Find 20 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGCTTCTGGTGGTCTTGTT+AGG | 0.322378 | 7.3:+47320465 | MS.gene83218:CDS |
| TGAAGTATATGAGATTTCTT+AGG | 0.358557 | 7.3:+47320495 | MS.gene83218:CDS |
| TGTGAAGAGATTTGTGCTTC+TGG | 0.391599 | 7.3:+47320452 | MS.gene83218:CDS |
| GATGCAAGGCACCACATGTT+AGG | 0.408002 | 7.3:+47320368 | MS.gene83218:CDS |
| CTGGGAGCCCTAAAATGAAT+AGG | 0.417019 | 7.3:-47320548 | None:intergenic |
| AATAGGACCATGAGAAGGTT+TGG | 0.442500 | 7.3:-47320531 | None:intergenic |
| ATATGAGATTTCTTAGGAAG+AGG | 0.471394 | 7.3:+47320501 | MS.gene83218:CDS |
| GTTGCAAAGGAGCTTCTCAA+TGG | 0.520484 | 7.3:+47320407 | MS.gene83218:CDS |
| ACTTGCCTCCATCGTTGCAA+AGG | 0.580774 | 7.3:+47320394 | MS.gene83218:CDS |
| GAGAAGCTCCTTTGCAACGA+TGG | 0.584142 | 7.3:-47320402 | None:intergenic |
| GAAGAGATTTGTGCTTCTGG+TGG | 0.586152 | 7.3:+47320455 | MS.gene83218:CDS |
| GGAGCTTCTCAATGGACAGA+AGG | 0.600116 | 7.3:+47320415 | MS.gene83218:CDS |
| AGGTATCTGCGCAAAGAGGG+TGG | 0.610561 | 7.3:+47320340 | MS.gene83218:CDS |
| TTCAGGTATCTGCGCAAAGA+GGG | 0.610853 | 7.3:+47320337 | MS.gene83218:CDS |
| AGAGGGTGGTGATCGATGCA+AGG | 0.618099 | 7.3:+47320354 | MS.gene83218:CDS |
| ATGAACACCAAACCTTCTCA+TGG | 0.620990 | 7.3:+47320524 | MS.gene83218:CDS |
| GTTCAGGTATCTGCGCAAAG+AGG | 0.631106 | 7.3:+47320336 | MS.gene83218:CDS |
| AAGCTCCTTTGCAACGATGG+AGG | 0.638563 | 7.3:-47320399 | None:intergenic |
| AAATGAATAGGACCATGAGA+AGG | 0.662695 | 7.3:-47320536 | None:intergenic |
| AGGCAAGTCTACCTAACATG+TGG | 0.695141 | 7.3:-47320379 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGAAGTATATGAGATTTCTT+AGG | + | chr7.3:47320495-47320514 | MS.gene83218:CDS | 25.0% |
| ATATGAGATTTCTTAGGAAG+AGG | + | chr7.3:47320501-47320520 | MS.gene83218:CDS | 30.0% | |
| ! | TCATGGTCCTATTCATTTTA+GGG | + | chr7.3:47320541-47320560 | MS.gene83218:CDS | 30.0% |
| AAATGAATAGGACCATGAGA+AGG | - | chr7.3:47320539-47320558 | None:intergenic | 35.0% | |
| ! | CTCATGGTCCTATTCATTTT+AGG | + | chr7.3:47320540-47320559 | MS.gene83218:CDS | 35.0% |
| AATAGGACCATGAGAAGGTT+TGG | - | chr7.3:47320534-47320553 | None:intergenic | 40.0% | |
| ATGAACACCAAACCTTCTCA+TGG | + | chr7.3:47320524-47320543 | MS.gene83218:CDS | 40.0% | |
| TGTGAAGAGATTTGTGCTTC+TGG | + | chr7.3:47320452-47320471 | MS.gene83218:CDS | 40.0% | |
| AGGCAAGTCTACCTAACATG+TGG | - | chr7.3:47320382-47320401 | None:intergenic | 45.0% | |
| ATACCTGAACCAGACACCAT+TGG | - | chr7.3:47320326-47320345 | None:intergenic | 45.0% | |
| GAAGAGATTTGTGCTTCTGG+TGG | + | chr7.3:47320455-47320474 | MS.gene83218:CDS | 45.0% | |
| GTTGCAAAGGAGCTTCTCAA+TGG | + | chr7.3:47320407-47320426 | MS.gene83218:CDS | 45.0% | |
| TTCAGGTATCTGCGCAAAGA+GGG | + | chr7.3:47320337-47320356 | MS.gene83218:CDS | 45.0% | |
| AAGCTCCTTTGCAACGATGG+AGG | - | chr7.3:47320402-47320421 | None:intergenic | 50.0% | |
| ACTTGCCTCCATCGTTGCAA+AGG | + | chr7.3:47320394-47320413 | MS.gene83218:CDS | 50.0% | |
| GAGAAGCTCCTTTGCAACGA+TGG | - | chr7.3:47320405-47320424 | None:intergenic | 50.0% | |
| GGAGCTTCTCAATGGACAGA+AGG | + | chr7.3:47320415-47320434 | MS.gene83218:CDS | 50.0% | |
| GTTCAGGTATCTGCGCAAAG+AGG | + | chr7.3:47320336-47320355 | MS.gene83218:CDS | 50.0% | |
| ! | GATGCAAGGCACCACATGTT+AGG | + | chr7.3:47320368-47320387 | MS.gene83218:CDS | 50.0% |
| !! | GTGCTTCTGGTGGTCTTGTT+AGG | + | chr7.3:47320465-47320484 | MS.gene83218:CDS | 50.0% |
| AGAGGGTGGTGATCGATGCA+AGG | + | chr7.3:47320354-47320373 | MS.gene83218:CDS | 55.0% | |
| AGGTATCTGCGCAAAGAGGG+TGG | + | chr7.3:47320340-47320359 | MS.gene83218:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 47320326 | 47320569 | 47320326 | ID=MS.gene83218 |
| chr7.3 | mRNA | 47320326 | 47320569 | 47320326 | ID=MS.gene83218.t1;Parent=MS.gene83218 |
| chr7.3 | exon | 47320326 | 47320569 | 47320326 | ID=MS.gene83218.t1.exon1;Parent=MS.gene83218.t1 |
| chr7.3 | CDS | 47320326 | 47320569 | 47320326 | ID=cds.MS.gene83218.t1;Parent=MS.gene83218.t1 |
| Gene Sequence |
| Protein sequence |
