Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87406.t1 | RHN45016.1 | 95.8 | 310 | 13 | 0 | 453 | 762 | 420 | 729 | 6.10E-179 | 637.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87406.t1 | Q9Y222 | 25.8 | 256 | 173 | 7 | 483 | 733 | 156 | 399 | 5.1e-17 | 91.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87406.t1 | A0A396GV57 | 95.8 | 310 | 13 | 0 | 453 | 762 | 420 | 729 | 4.4e-179 | 637.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene87406.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene87406 | 0.846856 | 1.67E-59 | -1.69E-46 |
| MS.gene049159 | MS.gene87406 | 0.826699 | 2.35E-54 | -1.69E-46 |
| MS.gene049368 | MS.gene87406 | 0.823449 | 1.38E-53 | -1.69E-46 |
| MS.gene049411 | MS.gene87406 | 0.80586 | 1.09E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene87406 | 0.806282 | 8.87E-50 | -1.69E-46 |
| MS.gene050384 | MS.gene87406 | 0.816623 | 5.03E-52 | -1.69E-46 |
| MS.gene050386 | MS.gene87406 | 0.822865 | 1.88E-53 | -1.69E-46 |
| MS.gene050551 | MS.gene87406 | 0.801763 | 7.72E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene87406 | 0.871484 | 6.60E-67 | -1.69E-46 |
| MS.gene050737 | MS.gene87406 | 0.815182 | 1.05E-51 | -1.69E-46 |
| MS.gene050815 | MS.gene87406 | 0.821597 | 3.71E-53 | -1.69E-46 |
| MS.gene050838 | MS.gene87406 | 0.820323 | 7.29E-53 | -1.69E-46 |
| MS.gene050941 | MS.gene87406 | 0.852631 | 4.07E-61 | -1.69E-46 |
| MS.gene051003 | MS.gene87406 | 0.82309 | 1.67E-53 | -1.69E-46 |
| MS.gene051102 | MS.gene87406 | 0.804838 | 1.78E-49 | -1.69E-46 |
| MS.gene051328 | MS.gene87406 | 0.841793 | 3.83E-58 | -1.69E-46 |
| MS.gene051642 | MS.gene87406 | 0.805401 | 1.36E-49 | -1.69E-46 |
| MS.gene051757 | MS.gene87406 | 0.811252 | 7.68E-51 | -1.69E-46 |
| MS.gene052307 | MS.gene87406 | 0.80319 | 3.92E-49 | -1.69E-46 |
| MS.gene052342 | MS.gene87406 | 0.811242 | 7.72E-51 | -1.69E-46 |
| MS.gene052397 | MS.gene87406 | 0.806283 | 8.87E-50 | -1.69E-46 |
| MS.gene052434 | MS.gene87406 | 0.820679 | 6.04E-53 | -1.69E-46 |
| MS.gene052516 | MS.gene87406 | 0.836083 | 1.15E-56 | -1.69E-46 |
| MS.gene052518 | MS.gene87406 | 0.825254 | 5.18E-54 | -1.69E-46 |
| MS.gene052863 | MS.gene87406 | 0.860733 | 1.68E-63 | -1.69E-46 |
| MS.gene052896 | MS.gene87406 | 0.83501 | 2.15E-56 | -1.69E-46 |
| MS.gene053008 | MS.gene87406 | 0.861549 | 9.49E-64 | -1.69E-46 |
| MS.gene053381 | MS.gene87406 | 0.837973 | 3.79E-57 | -1.69E-46 |
| MS.gene054472 | MS.gene87406 | 0.808758 | 2.65E-50 | -1.69E-46 |
| MS.gene05458 | MS.gene87406 | 0.819497 | 1.13E-52 | -1.69E-46 |
| MS.gene054823 | MS.gene87406 | 0.813992 | 1.93E-51 | -1.69E-46 |
| MS.gene05517 | MS.gene87406 | 0.811622 | 6.38E-51 | -1.69E-46 |
| MS.gene055311 | MS.gene87406 | 0.812325 | 4.49E-51 | -1.69E-46 |
| MS.gene055313 | MS.gene87406 | 0.806176 | 9.34E-50 | -1.69E-46 |
| MS.gene055468 | MS.gene87406 | 0.806311 | 8.75E-50 | -1.69E-46 |
| MS.gene055512 | MS.gene87406 | 0.802355 | 5.84E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene87406 | 0.829601 | 4.70E-55 | -1.69E-46 |
| MS.gene055623 | MS.gene87406 | 0.803496 | 3.39E-49 | -1.69E-46 |
| MS.gene055979 | MS.gene87406 | 0.858702 | 6.87E-63 | -1.69E-46 |
| MS.gene056181 | MS.gene87406 | 0.82888 | 7.03E-55 | -1.69E-46 |
| MS.gene056787 | MS.gene87406 | 0.802627 | 5.13E-49 | -1.69E-46 |
| MS.gene056831 | MS.gene87406 | 0.807525 | 4.84E-50 | -1.69E-46 |
| MS.gene057057 | MS.gene87406 | 0.810139 | 1.34E-50 | -1.69E-46 |
| MS.gene057140 | MS.gene87406 | 0.807531 | 4.83E-50 | -1.69E-46 |
| MS.gene057915 | MS.gene87406 | 0.810093 | 1.37E-50 | -1.69E-46 |
| MS.gene058646 | MS.gene87406 | 0.842564 | 2.39E-58 | -1.69E-46 |
| MS.gene058654 | MS.gene87406 | 0.819107 | 1.38E-52 | -1.69E-46 |
| MS.gene058906 | MS.gene87406 | 0.814493 | 1.50E-51 | -1.69E-46 |
| MS.gene059283 | MS.gene87406 | 0.815103 | 1.10E-51 | -1.69E-46 |
| MS.gene059842 | MS.gene87406 | 0.837777 | 4.25E-57 | -1.69E-46 |
| MS.gene06010 | MS.gene87406 | 0.812446 | 4.22E-51 | -1.69E-46 |
| MS.gene060870 | MS.gene87406 | 0.800251 | 1.57E-48 | -1.69E-46 |
| MS.gene060935 | MS.gene87406 | 0.802962 | 4.37E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87406.t1 | MTR_8g077990 | 82.413 | 779 | 34 | 6 | 1 | 762 | 1 | 693 | 0.0 | 1090 |
| MS.gene87406.t1 | MTR_1g076600 | 95.050 | 101 | 5 | 0 | 662 | 762 | 55 | 155 | 1.76e-66 | 217 |
| MS.gene87406.t1 | MTR_7g029435 | 93.478 | 92 | 6 | 0 | 671 | 762 | 1 | 92 | 7.53e-57 | 189 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87406.t1 | AT5G41020 | 55.124 | 283 | 126 | 1 | 477 | 759 | 302 | 583 | 1.88e-112 | 353 |
Find 128 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATAAATTCAAGCTCTTC+AGG | 0.254261 | 8.3:-26111238 | None:intergenic |
| ATCATCATCTTCACATTCAT+TGG | 0.267885 | 8.3:-26110375 | None:intergenic |
| AACAAATGATCCACCATATT+GGG | 0.286872 | 8.3:+26111722 | MS.gene87406:CDS |
| ACATTGCTTTCGGACATATA+TGG | 0.292466 | 8.3:-26109633 | None:intergenic |
| GAACAAATGATCCACCATAT+TGG | 0.329038 | 8.3:+26111721 | MS.gene87406:CDS |
| CTATATAGATTCTCATGGTT+TGG | 0.339430 | 8.3:+26111057 | MS.gene87406:CDS |
| TATATAGATTCTCATGGTTT+GGG | 0.352407 | 8.3:+26111058 | MS.gene87406:CDS |
| TCTCTATCTTTCAACAAGTT+GGG | 0.365789 | 8.3:-26111802 | None:intergenic |
| CTCTCTATCTTTCAACAAGT+TGG | 0.365952 | 8.3:-26111803 | None:intergenic |
| CAGATCAGTTACATTGCTTT+CGG | 0.367004 | 8.3:-26109643 | None:intergenic |
| TTATGTGTGCTTTCTTCACT+TGG | 0.367181 | 8.3:-26110140 | None:intergenic |
| TAGCCCTCTCAAGCAGATCT+AGG | 0.372193 | 8.3:-26111423 | None:intergenic |
| GATAGACGCTTTATATGCTT+TGG | 0.375237 | 8.3:+26111621 | MS.gene87406:CDS |
| ACTCCTAACTCTGCACATAA+TGG | 0.384709 | 8.3:+26110908 | MS.gene87406:CDS |
| TTTGATCTTCTCATCTTCTT+CGG | 0.386429 | 8.3:-26111020 | None:intergenic |
| GTTCCATTATGTGCAGAGTT+AGG | 0.391649 | 8.3:-26110911 | None:intergenic |
| CTGATCTGCCTTTGATCAAA+AGG | 0.395638 | 8.3:+26109658 | MS.gene87406:CDS |
| TGGCGGCATGCTCCCCAATA+TGG | 0.396320 | 8.3:-26111735 | None:intergenic |
| GTTTCATTCTGTGCACAGTT+AGG | 0.404650 | 8.3:-26110755 | None:intergenic |
| GGGAGACGAAGGTCTTGATA+TGG | 0.410993 | 8.3:+26111078 | MS.gene87406:CDS |
| TTCCAACAGCCGCTAACCTC+AGG | 0.416232 | 8.3:-26111121 | None:intergenic |
| AGAAGCAATGAAGGTGATAA+TGG | 0.417384 | 8.3:+26109894 | MS.gene87406:CDS |
| GTTTCATTATGTGCACAGTT+AGG | 0.421260 | 8.3:-26110623 | None:intergenic |
| GTTTCATTATGTGCACAGTT+AGG | 0.421260 | 8.3:-26110887 | None:intergenic |
| TATTCTTCTTCGTCTTGCCT+TGG | 0.426509 | 8.3:-26110451 | None:intergenic |
| GAGAGCAGTAGCTGACGCTT+TGG | 0.427556 | 8.3:+26111291 | MS.gene87406:CDS |
| TGCTCATACTTTGTTAGAAA+AGG | 0.429545 | 8.3:+26111195 | MS.gene87406:CDS |
| GTTGCGAGATAATATTTGTT+GGG | 0.434220 | 8.3:+26111477 | MS.gene87406:CDS |
| TCATCATCTTCACATTCATT+GGG | 0.442787 | 8.3:-26110374 | None:intergenic |
| AGAGCAGTAGCTGACGCTTT+GGG | 0.446113 | 8.3:+26111292 | MS.gene87406:CDS |
| GCTGCATGGAGGAGGTAGAC+TGG | 0.446516 | 8.3:+26111650 | MS.gene87406:CDS |
| GTCACTGATGTTAATGATCA+AGG | 0.447087 | 8.3:+26110683 | MS.gene87406:CDS |
| GTCACTGATGTTAATGATCA+AGG | 0.447087 | 8.3:+26110815 | MS.gene87406:CDS |
| CTAAACGCAAACAAGTAACT+TGG | 0.447239 | 8.3:+26110936 | MS.gene87406:CDS |
| CGCCAAGTCATTCATTGAGC+AGG | 0.451714 | 8.3:+26111753 | MS.gene87406:CDS |
| CATTGGTTGCTAGTGGTGAT+TGG | 0.452021 | 8.3:+26111575 | MS.gene87406:CDS |
| AATATTTGTTGGGAGGCAAT+TGG | 0.463198 | 8.3:+26111487 | MS.gene87406:CDS |
| GTCACTGAAGTTAATGATCA+AGG | 0.467385 | 8.3:+26110551 | MS.gene87406:CDS |
| CGTCGATACACACTCTCATA+AGG | 0.471062 | 8.3:-26111172 | None:intergenic |
| ACACTCTCATAAGGCCTCTC+AGG | 0.477449 | 8.3:-26111163 | None:intergenic |
| AAGAAGCACAAACTAATTGA+AGG | 0.478439 | 8.3:+26110221 | MS.gene87406:CDS |
| ACTAGCTCCATGTTGCTCAT+AGG | 0.479373 | 8.3:-26111266 | None:intergenic |
| TGCTTTGGATGCTTGCTGCA+TGG | 0.479442 | 8.3:+26111636 | MS.gene87406:CDS |
| AAAAGGAGGGAAGGTGTAAG+TGG | 0.481580 | 8.3:+26111212 | MS.gene87406:CDS |
| TAGAAGCAATGGAGGTGAAG+AGG | 0.481860 | 8.3:+26110271 | MS.gene87406:CDS |
| ACGGCTTATTGCGTGGGAAA+AGG | 0.484774 | 8.3:+26110990 | MS.gene87406:CDS |
| TGTTGCGAGATAATATTTGT+TGG | 0.489321 | 8.3:+26111476 | MS.gene87406:CDS |
| GGATGCTTGCTGCATGGAGG+AGG | 0.492182 | 8.3:+26111642 | MS.gene87406:CDS |
| ACCCTGAGGTTAGCGGCTGT+TGG | 0.493985 | 8.3:+26111119 | MS.gene87406:CDS |
| AAGTGAAGAAAGCACACATA+AGG | 0.495960 | 8.3:+26110142 | MS.gene87406:CDS |
| AAGTGAAGAAAGTAAACATA+AGG | 0.498172 | 8.3:+26110334 | MS.gene87406:CDS |
| TGAAGGTGATAATGGCGAGC+AGG | 0.499793 | 8.3:+26109902 | MS.gene87406:CDS |
| GAGTGACGAAAGCAAACATA+AGG | 0.503590 | 8.3:+26109953 | MS.gene87406:CDS |
| CTCTATCTTTCAACAAGTTG+GGG | 0.504361 | 8.3:-26111801 | None:intergenic |
| AGAAACTAATTGAGGAAGGT+GGG | 0.510841 | 8.3:+26110420 | MS.gene87406:CDS |
| TGTAATGACTTTAGAAGCAA+TGG | 0.511180 | 8.3:+26110260 | MS.gene87406:CDS |
| AAAAGCAATAAAAGTGAAGA+TGG | 0.511576 | 8.3:+26109702 | MS.gene87406:CDS |
| CTGCTCAATGAATGACTTGG+CGG | 0.516982 | 8.3:-26111752 | None:intergenic |
| AAGAAACTAATTGAGGAAGG+TGG | 0.519932 | 8.3:+26110419 | MS.gene87406:CDS |
| AATCTACTTGAGCATAGGTC+TGG | 0.523977 | 8.3:+26111676 | MS.gene87406:CDS |
| CTGCATGGAGGAGGTAGACT+GGG | 0.526890 | 8.3:+26111651 | MS.gene87406:CDS |
| TCAGCATCACCTTCATTGCT+AGG | 0.528214 | 8.3:-26110647 | None:intergenic |
| TCAGCATCACCTTCATTGCT+AGG | 0.528214 | 8.3:-26110779 | None:intergenic |
| TCATACTTTGTTAGAAAAGG+AGG | 0.529507 | 8.3:+26111198 | MS.gene87406:CDS |
| AATGAAGAAGAAACTAATTG+AGG | 0.530342 | 8.3:+26110412 | MS.gene87406:CDS |
| TTTGACTATATAGATTCTCA+TGG | 0.532297 | 8.3:+26111052 | MS.gene87406:CDS |
| AAGAAGCGGAAGCTAATTGA+AGG | 0.534553 | 8.3:+26109843 | MS.gene87406:CDS |
| CATATATGGATCGCCTCCAT+TGG | 0.534788 | 8.3:-26109619 | None:intergenic |
| GGAGGCAATTGGTCACAAGT+TGG | 0.536501 | 8.3:+26111498 | MS.gene87406:CDS |
| AAAAGTGAAGATGGCGAGCA+AGG | 0.538269 | 8.3:+26109711 | MS.gene87406:CDS |
| ATAGGTCTGGTGATGTGTGC+AGG | 0.541652 | 8.3:+26111689 | MS.gene87406:CDS |
| ATGAGCAACATGGAGCTAGT+TGG | 0.543870 | 8.3:+26111269 | MS.gene87406:CDS |
| AATCACAGGTTTGTTGTCAA+AGG | 0.550299 | 8.3:-26111827 | None:intergenic |
| TTTGGGTAAAAGTCGAGCTC+AGG | 0.550583 | 8.3:+26111309 | MS.gene87406:CDS |
| AGTAACTTGGGCTGATGAAG+TGG | 0.552578 | 8.3:+26110949 | MS.gene87406:CDS |
| AAGAAGAAACTAATTGAGGA+AGG | 0.552685 | 8.3:+26110416 | MS.gene87406:CDS |
| AATGAAACTCCTAGCAATGA+AGG | 0.554019 | 8.3:+26110638 | MS.gene87406:CDS |
| AATGAAACTCCTAGCAATGA+AGG | 0.554019 | 8.3:+26110770 | MS.gene87406:CDS |
| AAGAGAACTGAAGGCGAAGA+GGG | 0.556027 | 8.3:+26109591 | MS.gene87406:CDS |
| GACGGCTTTAGAAGCAATGA+AGG | 0.559488 | 8.3:+26109885 | MS.gene87406:CDS |
| TGTTGTGACGGCTTATTGCG+TGG | 0.561335 | 8.3:+26110983 | MS.gene87406:CDS |
| TGAGCAATTAACATCAACAT+TGG | 0.568371 | 8.3:+26111558 | MS.gene87406:CDS |
| TAAACCTAGATCTGCTTGAG+AGG | 0.570723 | 8.3:+26111419 | MS.gene87406:CDS |
| AGAAGCAATGGAGGTGAAGA+GGG | 0.572295 | 8.3:+26110272 | MS.gene87406:CDS |
| TAGAAACCTCAACAAATCAC+AGG | 0.579568 | 8.3:-26111841 | None:intergenic |
| GAAGGTGATAATGGCGAGCA+GGG | 0.580967 | 8.3:+26109903 | MS.gene87406:CDS |
| CATACTTTGTTAGAAAAGGA+GGG | 0.581182 | 8.3:+26111199 | MS.gene87406:CDS |
| ACATCAACATTGGTTGCTAG+TGG | 0.583463 | 8.3:+26111568 | MS.gene87406:CDS |
| AACAAACCTGTGATTTGTTG+AGG | 0.586181 | 8.3:+26111835 | MS.gene87406:CDS |
| GCGAGATAATATTTGTTGGG+AGG | 0.587211 | 8.3:+26111480 | MS.gene87406:CDS |
| CATATAAAGCGTCTATCAAG+CGG | 0.589919 | 8.3:-26111615 | None:intergenic |
| GGCGAAGAGGGTGAAACCAA+TGG | 0.590103 | 8.3:+26109603 | MS.gene87406:CDS |
| GAGGAGAAGTTATGTTGTGA+CGG | 0.591020 | 8.3:+26110971 | MS.gene87406:CDS |
| AAATTACACCCTGAGGTTAG+CGG | 0.591381 | 8.3:+26111112 | MS.gene87406:CDS |
| CTTTGTTAGAAAAGGAGGGA+AGG | 0.592917 | 8.3:+26111203 | MS.gene87406:CDS |
| CGGCATGCTCCCCAATATGG+TGG | 0.593209 | 8.3:-26111732 | None:intergenic |
| AGAAGCAATGATGATGAAGA+TGG | 0.594055 | 8.3:+26110080 | MS.gene87406:CDS |
| AAACCTAGATCTGCTTGAGA+GGG | 0.602034 | 8.3:+26111420 | MS.gene87406:CDS |
| TCCAACAGCCGCTAACCTCA+GGG | 0.602654 | 8.3:-26111120 | None:intergenic |
| TAAACGCAAACAAGTAACTT+GGG | 0.603412 | 8.3:+26110937 | MS.gene87406:CDS |
| CAACTTGTTGAAAGATAGAG+AGG | 0.613256 | 8.3:+26111804 | MS.gene87406:CDS |
| AACTTGGGCTGATGAAGTGG+AGG | 0.617904 | 8.3:+26110952 | MS.gene87406:CDS |
| GGGACAATCTACTTGAGCAT+AGG | 0.618965 | 8.3:+26111671 | MS.gene87406:CDS |
| CAAGAGAACTGAAGGCGAAG+AGG | 0.619279 | 8.3:+26109590 | MS.gene87406:CDS |
| GAAGGAAACACACTTGAGGA+AGG | 0.622295 | 8.3:+26109861 | MS.gene87406:CDS |
| ACAAATGATCCACCATATTG+GGG | 0.622830 | 8.3:+26111723 | MS.gene87406:CDS |
| TCTCATGGTTTGGGAGACGA+AGG | 0.623440 | 8.3:+26111067 | MS.gene87406:CDS |
| AACCTGCTCAATGAATGACT+TGG | 0.625939 | 8.3:-26111755 | None:intergenic |
| GTGATGTGTGCAGGAAACGA+TGG | 0.634053 | 8.3:+26111698 | MS.gene87406:CDS |
| GAAGGTGGGAATCGTGACCA+AGG | 0.636683 | 8.3:+26110434 | MS.gene87406:CDS |
| CTGCCTTTGATCAAAAGGAA+TGG | 0.637174 | 8.3:+26109663 | MS.gene87406:CDS |
| GAAAAGACCTATGAGCAACA+TGG | 0.638568 | 8.3:+26111259 | MS.gene87406:CDS |
| GTTGTGACGGCTTATTGCGT+GGG | 0.642851 | 8.3:+26110984 | MS.gene87406:CDS |
| TTTGGATGCTTGCTGCATGG+AGG | 0.643384 | 8.3:+26111639 | MS.gene87406:CDS |
| TGATGTGTGCAGGAAACGAT+GGG | 0.644218 | 8.3:+26111699 | MS.gene87406:CDS |
| AATGAATGTGAAGATGATGA+TGG | 0.645642 | 8.3:+26110377 | MS.gene87406:CDS |
| AATTGAAGGAAACACACTGA+AGG | 0.653637 | 8.3:+26110043 | MS.gene87406:CDS |
| AATGACTTTAGAAGCAATGG+AGG | 0.658645 | 8.3:+26110263 | MS.gene87406:CDS |
| AACACACTTGAGGAAGGTGA+CGG | 0.661228 | 8.3:+26109867 | MS.gene87406:CDS |
| AAATGTCAAATTACACCCTG+AGG | 0.666420 | 8.3:+26111105 | MS.gene87406:CDS |
| AAAAGGACATTGGTCCCAAG+AGG | 0.666914 | 8.3:+26111372 | MS.gene87406:CDS |
| AATTGAAGGAAACACACTTG+AGG | 0.669341 | 8.3:+26109857 | MS.gene87406:CDS |
| TGTGAAGATGATGATGGCCA+AGG | 0.683186 | 8.3:+26110383 | MS.gene87406:CDS |
| GAGCTCAGGTAAAAGATGCG+TGG | 0.687675 | 8.3:+26111323 | MS.gene87406:CDS |
| GAAGAGGGTGAAACCAATGG+AGG | 0.700449 | 8.3:+26109606 | MS.gene87406:CDS |
| GAATGTGAAGCTGATGACCA+AGG | 0.711673 | 8.3:+26109999 | MS.gene87406:CDS |
| AATGAATGTGAAGATGACCA+AGG | 0.719006 | 8.3:+26110185 | MS.gene87406:CDS |
| GAATGTGAAGATGATGACCA+AGG | 0.731540 | 8.3:+26109807 | MS.gene87406:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATACCAAAGCAAAAAA+AGG | + | chr8.3:26111355-26111374 | MS.gene87406:CDS | 20.0% |
| !!! | AATGATTTTAATAGCAAAGA+AGG | + | chr8.3:26110506-26110525 | MS.gene87406:CDS | 20.0% |
| ! | AAAAGCAATAAAAGTGAAGA+TGG | + | chr8.3:26109702-26109721 | MS.gene87406:CDS | 25.0% |
| ! | AAGTGAAGAAAGTAAACATA+AGG | + | chr8.3:26110334-26110353 | MS.gene87406:CDS | 25.0% |
| ! | AATGAAGAAGAAACTAATTG+AGG | + | chr8.3:26110412-26110431 | MS.gene87406:CDS | 25.0% |
| ! | ATGAAAAAGAAGCTAATTGA+AGG | + | chr8.3:26110029-26110048 | MS.gene87406:CDS | 25.0% |
| ! | TATATAGATTCTCATGGTTT+GGG | + | chr8.3:26111058-26111077 | MS.gene87406:CDS | 25.0% |
| ! | TTTGACTATATAGATTCTCA+TGG | + | chr8.3:26111052-26111071 | MS.gene87406:CDS | 25.0% |
| !!! | ATAAATTTTGATACTCCTCT+TGG | - | chr8.3:26111390-26111409 | None:intergenic | 25.0% |
| !!! | TAAATTTTGATACTCCTCTT+GGG | - | chr8.3:26111389-26111408 | None:intergenic | 25.0% |
| AACAAATGATCCACCATATT+GGG | + | chr8.3:26111722-26111741 | MS.gene87406:CDS | 30.0% | |
| AAGAAAAACAAGAGAACTGA+AGG | + | chr8.3:26109582-26109601 | MS.gene87406:CDS | 30.0% | |
| AAGAAGAAACTAATTGAGGA+AGG | + | chr8.3:26110416-26110435 | MS.gene87406:CDS | 30.0% | |
| AATGAATGTGAAGATGATGA+TGG | + | chr8.3:26109804-26109823 | MS.gene87406:CDS | 30.0% | |
| ATCATCATCTTCACATTCAT+TGG | - | chr8.3:26110378-26110397 | None:intergenic | 30.0% | |
| CATACTTTGTTAGAAAAGGA+GGG | + | chr8.3:26111199-26111218 | MS.gene87406:CDS | 30.0% | |
| CCTTATAAAAAATCACAGCA+TGG | + | chr8.3:26111451-26111470 | MS.gene87406:CDS | 30.0% | |
| CTATATAGATTCTCATGGTT+TGG | + | chr8.3:26111057-26111076 | MS.gene87406:CDS | 30.0% | |
| GAAAGAAGATGAAAAAGAAG+CGG | + | chr8.3:26109829-26109848 | MS.gene87406:CDS | 30.0% | |
| GTGTCAGAAAAAGAAAAGTA+AGG | + | chr8.3:26110478-26110497 | MS.gene87406:CDS | 30.0% | |
| GTTGCGAGATAATATTTGTT+GGG | + | chr8.3:26111477-26111496 | MS.gene87406:CDS | 30.0% | |
| TAAACGCAAACAAGTAACTT+GGG | + | chr8.3:26110937-26110956 | MS.gene87406:CDS | 30.0% | |
| TCAATAAATTCAAGCTCTTC+AGG | - | chr8.3:26111241-26111260 | None:intergenic | 30.0% | |
| TCATACTTTGTTAGAAAAGG+AGG | + | chr8.3:26111198-26111217 | MS.gene87406:CDS | 30.0% | |
| TCATCATCTTCACATTCATT+GGG | - | chr8.3:26109804-26109823 | None:intergenic | 30.0% | |
| TCTCTATCTTTCAACAAGTT+GGG | - | chr8.3:26111805-26111824 | None:intergenic | 30.0% | |
| TGAGCAATTAACATCAACAT+TGG | + | chr8.3:26111558-26111577 | MS.gene87406:CDS | 30.0% | |
| TGCTCATACTTTGTTAGAAA+AGG | + | chr8.3:26111195-26111214 | MS.gene87406:CDS | 30.0% | |
| TGTTGCGAGATAATATTTGT+TGG | + | chr8.3:26111476-26111495 | MS.gene87406:CDS | 30.0% | |
| TTTGATCTTCTCATCTTCTT+CGG | - | chr8.3:26111023-26111042 | None:intergenic | 30.0% | |
| ! | AAGAAGCACAAACTAATTGA+AGG | + | chr8.3:26110221-26110240 | MS.gene87406:CDS | 30.0% |
| ! | TCTTCTTCATTTTCTTTCCT+TGG | - | chr8.3:26110403-26110422 | None:intergenic | 30.0% |
| ! | TGTAATGACTTTAGAAGCAA+TGG | + | chr8.3:26110260-26110279 | MS.gene87406:CDS | 30.0% |
| !! | TCTTTTTCATCTTCTTTCCT+TGG | - | chr8.3:26109827-26109846 | None:intergenic | 30.0% |
| !! | TCTTTTTCGTCTTTTTTCCT+TGG | - | chr8.3:26110205-26110224 | None:intergenic | 30.0% |
| !!! | CCATGCTGTGATTTTTTATA+AGG | - | chr8.3:26111454-26111473 | None:intergenic | 30.0% |
| AAATGTCAAATTACACCCTG+AGG | + | chr8.3:26111105-26111124 | MS.gene87406:CDS | 35.0% | |
| AAGAAACTAATTGAGGAAGG+TGG | + | chr8.3:26110419-26110438 | MS.gene87406:CDS | 35.0% | |
| AATGAAACTCCTAGCAATGA+AGG | + | chr8.3:26110638-26110657 | MS.gene87406:CDS | 35.0% | |
| AATGAATGTGAAGATGACCA+AGG | + | chr8.3:26110185-26110204 | MS.gene87406:CDS | 35.0% | |
| AATTGAAGGAAACACACTGA+AGG | + | chr8.3:26110043-26110062 | MS.gene87406:CDS | 35.0% | |
| AATTGAAGGAAACACACTTG+AGG | + | chr8.3:26109857-26109876 | MS.gene87406:CDS | 35.0% | |
| ACAAATGATCCACCATATTG+GGG | + | chr8.3:26111723-26111742 | MS.gene87406:CDS | 35.0% | |
| ACATTGCTTTCGGACATATA+TGG | - | chr8.3:26109636-26109655 | None:intergenic | 35.0% | |
| AGAAACTAATTGAGGAAGGT+GGG | + | chr8.3:26110420-26110439 | MS.gene87406:CDS | 35.0% | |
| AGAAGCAATGAAGGTGATAA+TGG | + | chr8.3:26109894-26109913 | MS.gene87406:CDS | 35.0% | |
| CAGATCAGTTACATTGCTTT+CGG | - | chr8.3:26109646-26109665 | None:intergenic | 35.0% | |
| CCAAAGCAAAAAAAGGACAT+TGG | + | chr8.3:26111362-26111381 | MS.gene87406:CDS | 35.0% | |
| CTAAACGCAAACAAGTAACT+TGG | + | chr8.3:26110936-26110955 | MS.gene87406:CDS | 35.0% | |
| CTCTATCTTTCAACAAGTTG+GGG | - | chr8.3:26111804-26111823 | None:intergenic | 35.0% | |
| CTCTCTATCTTTCAACAAGT+TGG | - | chr8.3:26111806-26111825 | None:intergenic | 35.0% | |
| GAACAAATGATCCACCATAT+TGG | + | chr8.3:26111721-26111740 | MS.gene87406:CDS | 35.0% | |
| GATAGACGCTTTATATGCTT+TGG | + | chr8.3:26111621-26111640 | MS.gene87406:CDS | 35.0% | |
| GTCACTGAAGTTAATGATCA+AGG | + | chr8.3:26110551-26110570 | MS.gene87406:CDS | 35.0% | |
| GTCACTGATGTTAATGATCA+AGG | + | chr8.3:26110683-26110702 | MS.gene87406:CDS | 35.0% | |
| GTTTCATTATGTGCACAGTT+AGG | - | chr8.3:26110626-26110645 | None:intergenic | 35.0% | |
| TTATGTGTGCTTTCTTCACT+TGG | - | chr8.3:26110143-26110162 | None:intergenic | 35.0% | |
| ! | AAGTGAAGAAAGCACACATA+AGG | + | chr8.3:26110142-26110161 | MS.gene87406:CDS | 35.0% |
| ! | AATATTTGTTGGGAGGCAAT+TGG | + | chr8.3:26111487-26111506 | MS.gene87406:CDS | 35.0% |
| ! | AATGACTTTAGAAGCAATGG+AGG | + | chr8.3:26110263-26110282 | MS.gene87406:CDS | 35.0% |
| ! | AGAAGCAATGATGATGAAGA+TGG | + | chr8.3:26110080-26110099 | MS.gene87406:CDS | 35.0% |
| ! | CATATAAAGCGTCTATCAAG+CGG | - | chr8.3:26111618-26111637 | None:intergenic | 35.0% |
| ! | TCACCATTCCTTTTGATCAA+AGG | - | chr8.3:26109669-26109688 | None:intergenic | 35.0% |
| !! | AATCACAGGTTTGTTGTCAA+AGG | - | chr8.3:26111830-26111849 | None:intergenic | 35.0% |
| !! | CAACTTGTTGAAAGATAGAG+AGG | + | chr8.3:26111804-26111823 | MS.gene87406:CDS | 35.0% |
| !!! | CCAATGTCCTTTTTTTGCTT+TGG | - | chr8.3:26111365-26111384 | None:intergenic | 35.0% |
| !!! | GTTCTCTTGTTTTTCTTGCT+CGG | - | chr8.3:26109579-26109598 | None:intergenic | 35.0% |
| AAACCTAGATCTGCTTGAGA+GGG | + | chr8.3:26111420-26111439 | MS.gene87406:CDS | 40.0% | |
| AAATTACACCCTGAGGTTAG+CGG | + | chr8.3:26111112-26111131 | MS.gene87406:CDS | 40.0% | |
| AACCTGCTCAATGAATGACT+TGG | - | chr8.3:26111758-26111777 | None:intergenic | 40.0% | |
| AAGAAGCGGAAGCTAATTGA+AGG | + | chr8.3:26109843-26109862 | MS.gene87406:CDS | 40.0% | |
| AATCTACTTGAGCATAGGTC+TGG | + | chr8.3:26111676-26111695 | MS.gene87406:CDS | 40.0% | |
| ACTCCTAACTCTGCACATAA+TGG | + | chr8.3:26110908-26110927 | MS.gene87406:CDS | 40.0% | |
| CTGATCTGCCTTTGATCAAA+AGG | + | chr8.3:26109658-26109677 | MS.gene87406:CDS | 40.0% | |
| CTGCCTTTGATCAAAAGGAA+TGG | + | chr8.3:26109663-26109682 | MS.gene87406:CDS | 40.0% | |
| CTTTGTTAGAAAAGGAGGGA+AGG | + | chr8.3:26111203-26111222 | MS.gene87406:CDS | 40.0% | |
| GAAAAGACCTATGAGCAACA+TGG | + | chr8.3:26111259-26111278 | MS.gene87406:CDS | 40.0% | |
| GAATGTGAAGATGATGACCA+AGG | + | chr8.3:26109807-26109826 | MS.gene87406:CDS | 40.0% | |
| GAGGAGAAGTTATGTTGTGA+CGG | + | chr8.3:26110971-26110990 | MS.gene87406:CDS | 40.0% | |
| GAGTGACGAAAGCAAACATA+AGG | + | chr8.3:26109953-26109972 | MS.gene87406:CDS | 40.0% | |
| GTTCCATTATGTGCAGAGTT+AGG | - | chr8.3:26110914-26110933 | None:intergenic | 40.0% | |
| GTTTCATTCTGTGCACAGTT+AGG | - | chr8.3:26110758-26110777 | None:intergenic | 40.0% | |
| TAAACCTAGATCTGCTTGAG+AGG | + | chr8.3:26111419-26111438 | MS.gene87406:CDS | 40.0% | |
| TATTCTTCTTCGTCTTGCCT+TGG | - | chr8.3:26110454-26110473 | None:intergenic | 40.0% | |
| ! | ACATCAACATTGGTTGCTAG+TGG | + | chr8.3:26111568-26111587 | MS.gene87406:CDS | 40.0% |
| ! | GCGAGATAATATTTGTTGGG+AGG | + | chr8.3:26111480-26111499 | MS.gene87406:CDS | 40.0% |
| ! | TTGCAAAAAGCATACCTGAG+AGG | + | chr8.3:26111149-26111168 | MS.gene87406:CDS | 40.0% |
| AAAAGGACATTGGTCCCAAG+AGG | + | chr8.3:26111372-26111391 | MS.gene87406:CDS | 45.0% | |
| AAAAGTGAAGATGGCGAGCA+AGG | + | chr8.3:26109711-26109730 | MS.gene87406:CDS | 45.0% | |
| AACACACTTGAGGAAGGTGA+CGG | + | chr8.3:26109867-26109886 | MS.gene87406:CDS | 45.0% | |
| AAGAGAACTGAAGGCGAAGA+GGG | + | chr8.3:26109591-26109610 | MS.gene87406:CDS | 45.0% | |
| ACTAGCTCCATGTTGCTCAT+AGG | - | chr8.3:26111269-26111288 | None:intergenic | 45.0% | |
| AGAAGCAATGGAGGTGAAGA+GGG | + | chr8.3:26110272-26110291 | MS.gene87406:CDS | 45.0% | |
| AGTAACTTGGGCTGATGAAG+TGG | + | chr8.3:26110949-26110968 | MS.gene87406:CDS | 45.0% | |
| ATGAGCAACATGGAGCTAGT+TGG | + | chr8.3:26111269-26111288 | MS.gene87406:CDS | 45.0% | |
| CATATATGGATCGCCTCCAT+TGG | - | chr8.3:26109622-26109641 | None:intergenic | 45.0% | |
| CGTCGATACACACTCTCATA+AGG | - | chr8.3:26111175-26111194 | None:intergenic | 45.0% | |
| GAAGGAAACACACTTGAGGA+AGG | + | chr8.3:26109861-26109880 | MS.gene87406:CDS | 45.0% | |
| GAATGTGAAGCTGATGACCA+AGG | + | chr8.3:26109999-26110018 | MS.gene87406:CDS | 45.0% | |
| GGGACAATCTACTTGAGCAT+AGG | + | chr8.3:26111671-26111690 | MS.gene87406:CDS | 45.0% | |
| TAGAAGCAATGGAGGTGAAG+AGG | + | chr8.3:26110271-26110290 | MS.gene87406:CDS | 45.0% | |
| TCAGCATCACCTTCATTGCT+AGG | - | chr8.3:26110650-26110669 | None:intergenic | 45.0% | |
| TGATGTGTGCAGGAAACGAT+GGG | + | chr8.3:26111699-26111718 | MS.gene87406:CDS | 45.0% | |
| TTTGGGTAAAAGTCGAGCTC+AGG | + | chr8.3:26111309-26111328 | MS.gene87406:CDS | 45.0% | |
| ! | AAAAGGAGGGAAGGTGTAAG+TGG | + | chr8.3:26111212-26111231 | MS.gene87406:CDS | 45.0% |
| ! | CATTGGTTGCTAGTGGTGAT+TGG | + | chr8.3:26111575-26111594 | MS.gene87406:CDS | 45.0% |
| ! | CTGCTCAATGAATGACTTGG+CGG | - | chr8.3:26111755-26111774 | None:intergenic | 45.0% |
| ! | GTGCATTTTGCTGCAAGAAG+TGG | + | chr8.3:26111533-26111552 | MS.gene87406:CDS | 45.0% |
| !! | GACGGCTTTAGAAGCAATGA+AGG | + | chr8.3:26109885-26109904 | MS.gene87406:CDS | 45.0% |
| !! | TGTGAAGATGATGATGGCCA+AGG | + | chr8.3:26110383-26110402 | MS.gene87406:CDS | 45.0% |
| AACTTGGGCTGATGAAGTGG+AGG | + | chr8.3:26110952-26110971 | MS.gene87406:CDS | 50.0% | |
| ACACTCTCATAAGGCCTCTC+AGG | - | chr8.3:26111166-26111185 | None:intergenic | 50.0% | |
| ACGGCTTATTGCGTGGGAAA+AGG | + | chr8.3:26110990-26111009 | MS.gene87406:CDS | 50.0% | |
| AGAGCAGTAGCTGACGCTTT+GGG | + | chr8.3:26111292-26111311 | MS.gene87406:CDS | 50.0% | |
| CAAGAGAACTGAAGGCGAAG+AGG | + | chr8.3:26109590-26109609 | MS.gene87406:CDS | 50.0% | |
| CGCCAAGTCATTCATTGAGC+AGG | + | chr8.3:26111753-26111772 | MS.gene87406:CDS | 50.0% | |
| GAAGAGGGTGAAACCAATGG+AGG | + | chr8.3:26109606-26109625 | MS.gene87406:CDS | 50.0% | |
| GAGCTCAGGTAAAAGATGCG+TGG | + | chr8.3:26111323-26111342 | MS.gene87406:CDS | 50.0% | |
| GGAGGCAATTGGTCACAAGT+TGG | + | chr8.3:26111498-26111517 | MS.gene87406:CDS | 50.0% | |
| GGGAGACGAAGGTCTTGATA+TGG | + | chr8.3:26111078-26111097 | MS.gene87406:CDS | 50.0% | |
| GTGATGTGTGCAGGAAACGA+TGG | + | chr8.3:26111698-26111717 | MS.gene87406:CDS | 50.0% | |
| GTTGTGACGGCTTATTGCGT+GGG | + | chr8.3:26110984-26111003 | MS.gene87406:CDS | 50.0% | |
| TAGCCCTCTCAAGCAGATCT+AGG | - | chr8.3:26111426-26111445 | None:intergenic | 50.0% | |
| TGTTGTGACGGCTTATTGCG+TGG | + | chr8.3:26110983-26111002 | MS.gene87406:CDS | 50.0% | |
| ! | ATAGGTCTGGTGATGTGTGC+AGG | + | chr8.3:26111689-26111708 | MS.gene87406:CDS | 50.0% |
| ! | TCTCATGGTTTGGGAGACGA+AGG | + | chr8.3:26111067-26111086 | MS.gene87406:CDS | 50.0% |
| ! | TGCTTTGGATGCTTGCTGCA+TGG | + | chr8.3:26111636-26111655 | MS.gene87406:CDS | 50.0% |
| ! | TTTGGATGCTTGCTGCATGG+AGG | + | chr8.3:26111639-26111658 | MS.gene87406:CDS | 50.0% |
| !! | GAAGGTGATAATGGCGAGCA+GGG | + | chr8.3:26109903-26109922 | MS.gene87406:CDS | 50.0% |
| !! | TGAAGGTGATAATGGCGAGC+AGG | + | chr8.3:26109902-26109921 | MS.gene87406:CDS | 50.0% |
| CTGCATGGAGGAGGTAGACT+GGG | + | chr8.3:26111651-26111670 | MS.gene87406:CDS | 55.0% | |
| GAAGGTGGGAATCGTGACCA+AGG | + | chr8.3:26110434-26110453 | MS.gene87406:CDS | 55.0% | |
| GAGAGCAGTAGCTGACGCTT+TGG | + | chr8.3:26111291-26111310 | MS.gene87406:CDS | 55.0% | |
| GGCGAAGAGGGTGAAACCAA+TGG | + | chr8.3:26109603-26109622 | MS.gene87406:CDS | 55.0% | |
| TCCAACAGCCGCTAACCTCA+GGG | - | chr8.3:26111123-26111142 | None:intergenic | 55.0% | |
| TTCCAACAGCCGCTAACCTC+AGG | - | chr8.3:26111124-26111143 | None:intergenic | 55.0% | |
| ACCCTGAGGTTAGCGGCTGT+TGG | + | chr8.3:26111119-26111138 | MS.gene87406:CDS | 60.0% | |
| CGGCATGCTCCCCAATATGG+TGG | - | chr8.3:26111735-26111754 | None:intergenic | 60.0% | |
| GCTGCATGGAGGAGGTAGAC+TGG | + | chr8.3:26111650-26111669 | MS.gene87406:CDS | 60.0% | |
| TGGCGGCATGCTCCCCAATA+TGG | - | chr8.3:26111738-26111757 | None:intergenic | 60.0% | |
| ! | GGATGCTTGCTGCATGGAGG+AGG | + | chr8.3:26111642-26111661 | MS.gene87406:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 26109567 | 26111855 | 26109567 | ID=MS.gene87406 |
| chr8.3 | mRNA | 26109567 | 26111855 | 26109567 | ID=MS.gene87406.t1;Parent=MS.gene87406 |
| chr8.3 | exon | 26109567 | 26111855 | 26109567 | ID=MS.gene87406.t1.exon1;Parent=MS.gene87406.t1 |
| chr8.3 | CDS | 26109567 | 26111855 | 26109567 | ID=cds.MS.gene87406.t1;Parent=MS.gene87406.t1 |
| Gene Sequence |
| Protein sequence |
