AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88437


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88437.t1 MTR_8g040630 100.000 188 0 0 1 188 30 217 7.67e-142 395
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88437.t1 AT3G12300 87.766 188 23 0 1 188 1 188 4.37e-129 362

Find 55 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACATTTGAGATTCAAGTTT+TGG 0.209840 8.3:-57416582 MS.gene88437:CDS
ATTAGTCCCATATGCTTTCT+TGG 0.233780 8.3:+57416158 None:intergenic
TCTAATGTACTGGAAATAAT+TGG 0.268445 8.3:-57416713 MS.gene88437:CDS
ATACCCAAGGCTGTATAATA+TGG 0.289391 8.3:+57417206 None:intergenic
TGGTATAAAACTTCCATTCT+TGG 0.289803 8.3:-57416636 MS.gene88437:CDS
CATCTTGTGGTCGCTTAATA+TGG 0.311001 8.3:+57416747 None:intergenic
GGAGCAAACCTTTGCAGATA+TGG 0.317310 8.3:-57417082 MS.gene88437:CDS
TAAAGTCAGCCAGGTTAAAC+TGG 0.353864 8.3:+57416181 None:intergenic
TTTCCATATTATACAGCCTT+GGG 0.401172 8.3:-57417209 MS.gene88437:intron
TGTGGAGACACTACGAGTTC+AGG 0.427848 8.3:-57416133 MS.gene88437:CDS
GACTTTACCAAGAAAGCATA+TGG 0.429193 8.3:-57416165 MS.gene88437:CDS
TTCACAATCATAACCAAGAA+TGG 0.450408 8.3:+57416623 None:intergenic
AGTGTGGCAGCTGGGTCAGC+CGG 0.451970 8.3:+57416659 None:intergenic
CTTTCCATATTATACAGCCT+TGG 0.463889 8.3:-57417210 MS.gene88437:CDS
AGAAATAATGAAAATGTCAT+TGG 0.467147 8.3:-57415921 MS.gene88437:CDS
TTTATACCAAGTGTGGCAGC+TGG 0.471135 8.3:+57416650 None:intergenic
CAAGTTGTACCTTCCAATGC+AGG 0.471690 8.3:-57416034 MS.gene88437:intron
TTCAAGAACACATTCCAGTC+TGG 0.472331 8.3:-57417237 MS.gene88437:CDS
TTGCACTATGCCACTGAGAA+TGG 0.489210 8.3:-57416223 MS.gene88437:CDS
AGACATACAATCTAATGTAC+TGG 0.495892 8.3:-57416723 MS.gene88437:CDS
GATTGTATGTCTTCATCTTG+TGG 0.496368 8.3:+57416734 None:intergenic
CTTTCTTGGTAAAGTCAGCC+AGG 0.501342 8.3:+57416172 None:intergenic
TGGAGGATGGAATTGATGCC+AGG 0.511205 8.3:-57415901 MS.gene88437:CDS
GCTGACCCAGCTGCCACACT+TGG 0.511732 8.3:-57416656 MS.gene88437:CDS
ATGAAAATGTCATTGGAGGA+TGG 0.513519 8.3:-57415914 MS.gene88437:CDS
GAGCAAACCTTTGCAGATAT+GGG 0.519182 8.3:-57417081 MS.gene88437:CDS
TTCTCAAGCGACAGTTTGCA+TGG 0.520738 8.3:+57416109 None:intergenic
GTGTGGCAGCTGGGTCAGCC+GGG 0.525412 8.3:+57416660 None:intergenic
CAGACATCTCATACCTGCAT+TGG 0.527544 8.3:+57416021 None:intergenic
GGAAGGTACAACTTGAACTC+TGG 0.537228 8.3:+57416042 None:intergenic
AATAATGAAAATGTCATTGG+AGG 0.544133 8.3:-57415918 MS.gene88437:CDS
CTTCTCTGATCGTCTCTACT+CGG 0.546100 8.3:-57416079 MS.gene88437:CDS
GTCCACATACATTACGTGCC+CGG 0.551642 8.3:-57416678 MS.gene88437:CDS
TATGCCACTGAGAATGGATG+AGG 0.552706 8.3:-57416217 MS.gene88437:CDS
CATAACATTCAACCATCTCC+TGG 0.552872 8.3:+57415883 None:intergenic
TCTTTGTCCCATATCTGCAA+AGG 0.557834 8.3:+57417074 None:intergenic
TGATCGTCTCTACTCGGAAG+AGG 0.559398 8.3:-57416073 MS.gene88437:CDS
AGCATATGGGACTAATTATG+TGG 0.561284 8.3:-57416151 MS.gene88437:CDS
AGCCGGGCACGTAATGTATG+TGG 0.564997 8.3:+57416676 None:intergenic
CATCTCATACCTGCATTGGA+AGG 0.576069 8.3:+57416025 None:intergenic
ATGCAAACTGTCGCTTGAGA+AGG 0.579025 8.3:-57416107 MS.gene88437:CDS
AGGTACAACTTGAACTCTGG+TGG 0.579778 8.3:+57416045 None:intergenic
GAATCAAATCCAGTTTAACC+TGG 0.582198 8.3:-57416190 MS.gene88437:CDS
AGTGGCATAGTGCAAATGTA+CGG 0.583812 8.3:+57416231 None:intergenic
CACTGAGAATGGATGAGGGT+TGG 0.589041 8.3:-57416212 MS.gene88437:CDS
TTGCAGATATGGGACAAAGA+AGG 0.596515 8.3:-57417071 MS.gene88437:intron
ATATGGAAAGAAATCCAGAC+TGG 0.597297 8.3:+57417223 None:intergenic
TCTGCATGATGAACAATACA+AGG 0.597722 8.3:+57415941 None:intergenic
ACTTTACCAAGAAAGCATAT+GGG 0.601556 8.3:-57416164 MS.gene88437:CDS
TGTTATGTGTAGTTGTGTGT+CGG 0.606396 8.3:-57415867 MS.gene88437:CDS
ATGGAATTGATGCCAGGAGA+TGG 0.612954 8.3:-57415895 MS.gene88437:CDS
TTATACCAAGTGTGGCAGCT+GGG 0.613588 8.3:+57416651 None:intergenic
GGTACAACTTGAACTCTGGT+GGG 0.629720 8.3:+57416046 None:intergenic
CAACCCTCATCCATTCTCAG+TGG 0.706213 8.3:+57416213 None:intergenic
ATGCCACTGAGAATGGATGA+GGG 0.727443 8.3:-57416216 MS.gene88437:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATTTTTTTTATTATTGT+AGG - chr8.3:57415990-57416009 MS.gene88437:intron 10.0%
!!! TTTTATTATTTTTATGTCAT+AGG - chr8.3:57416822-57416841 MS.gene88437:intron 10.0%
!! TAATCAAAGTAAAATGATTT+CGG + chr8.3:57416128-57416147 None:intergenic 15.0%
!! AAGTTCTCCATATAATTAAA+TGG + chr8.3:57416675-57416694 None:intergenic 20.0%
!! AGAAATAATGAAAATGTCAT+TGG - chr8.3:57417172-57417191 MS.gene88437:intron 20.0%
!! CTTTCTTCCATTTAATTATA+TGG - chr8.3:57416665-57416684 MS.gene88437:CDS 20.0%
! AAATTAAACATCTGCAAGAT+TGG + chr8.3:57416621-57416640 None:intergenic 25.0%
! AATAATGAAAATGTCATTGG+AGG - chr8.3:57417175-57417194 MS.gene88437:intron 25.0%
! TCTAATGTACTGGAAATAAT+TGG - chr8.3:57416380-57416399 MS.gene88437:intron 25.0%
!! TAAACTTACGATTTTGTCTT+GGG - chr8.3:57416299-57416318 MS.gene88437:intron 25.0%
!! TTAAACTTACGATTTTGTCT+TGG - chr8.3:57416298-57416317 MS.gene88437:intron 25.0%
!!! TCATATGTTTGTTGTGATTA+AGG - chr8.3:57415944-57415963 MS.gene88437:CDS 25.0%
AGACATACAATCTAATGTAC+TGG - chr8.3:57416370-57416389 MS.gene88437:intron 30.0%
TGGTATAAAACTTCCATTCT+TGG - chr8.3:57416457-57416476 MS.gene88437:intron 30.0%
TTCACAATCATAACCAAGAA+TGG + chr8.3:57416473-57416492 None:intergenic 30.0%
TTTCCATATTATACAGCCTT+GGG - chr8.3:57415884-57415903 MS.gene88437:CDS 30.0%
! AAACTTACGATTTTGTCTTG+GGG - chr8.3:57416300-57416319 MS.gene88437:intron 30.0%
! ACTCTTTTTATAGTTGTGGA+TGG - chr8.3:57416323-57416342 MS.gene88437:intron 30.0%
! ACTTTACCAAGAAAGCATAT+GGG - chr8.3:57416929-57416948 MS.gene88437:intron 30.0%
!! CACATTTGAGATTCAAGTTT+TGG - chr8.3:57416511-57416530 MS.gene88437:intron 30.0%
AATATGTTGAAGAGTGACTG+AGG - chr8.3:57416257-57416276 MS.gene88437:intron 35.0%
AGCATATGGGACTAATTATG+TGG - chr8.3:57416942-57416961 MS.gene88437:intron 35.0%
ATACCCAAGGCTGTATAATA+TGG + chr8.3:57415890-57415909 None:intergenic 35.0%
ATATGGAAAGAAATCCAGAC+TGG + chr8.3:57415873-57415892 None:intergenic 35.0%
ATATGTTGAAGAGTGACTGA+GGG - chr8.3:57416258-57416277 MS.gene88437:intron 35.0%
ATGAAAATGTCATTGGAGGA+TGG - chr8.3:57417179-57417198 MS.gene88437:intron 35.0%
ATTAGTCCCATATGCTTTCT+TGG + chr8.3:57416938-57416957 None:intergenic 35.0%
CCTATAAGACACACAAACAT+GGG + chr8.3:57416594-57416613 None:intergenic 35.0%
CTTTCCATATTATACAGCCT+TGG - chr8.3:57415883-57415902 MS.gene88437:CDS 35.0%
GAATCAAATCCAGTTTAACC+TGG - chr8.3:57416903-57416922 MS.gene88437:intron 35.0%
GATTGTATGTCTTCATCTTG+TGG + chr8.3:57416362-57416381 None:intergenic 35.0%
TCCTATAAGACACACAAACA+TGG + chr8.3:57416595-57416614 None:intergenic 35.0%
TCTGCATGATGAACAATACA+AGG + chr8.3:57417155-57417174 None:intergenic 35.0%
TGTTATGTGTAGTTGTGTGT+CGG - chr8.3:57417226-57417245 MS.gene88437:CDS 35.0%
TTGTTAGCAGTGACATTAGA+TGG - chr8.3:57416056-57416075 MS.gene88437:CDS 35.0%
TTTCATCTCAATTGCATGTC+AGG - chr8.3:57417124-57417143 MS.gene88437:intron 35.0%
! GACTTTACCAAGAAAGCATA+TGG - chr8.3:57416928-57416947 MS.gene88437:intron 35.0%
! GTTTAGTTGTGCTTCTGAAA+TGG - chr8.3:57416172-57416191 MS.gene88437:CDS 35.0%
!! TGGAAGTTTTATACCAAGTG+TGG + chr8.3:57416453-57416472 None:intergenic 35.0%
AATGGACAGCACAACACATA+AGG + chr8.3:57416209-57416228 None:intergenic 40.0%
AGTGGCATAGTGCAAATGTA+CGG + chr8.3:57416865-57416884 None:intergenic 40.0%
CACATCAGCCTTTCATAACA+TGG + chr8.3:57416235-57416254 None:intergenic 40.0%
CATAACATTCAACCATCTCC+TGG + chr8.3:57417213-57417232 None:intergenic 40.0%
CATCTTGTGGTCGCTTAATA+TGG + chr8.3:57416349-57416368 None:intergenic 40.0%
CCATTTGGCCATGTTATGAA+AGG - chr8.3:57416224-57416243 MS.gene88437:CDS 40.0%
CCCATGTTTGTGTGTCTTAT+AGG - chr8.3:57416591-57416610 MS.gene88437:CDS 40.0%
CCTTTCATAACATGGCCAAA+TGG + chr8.3:57416227-57416246 None:intergenic 40.0%
GAGCAAACCTTTGCAGATAT+GGG - chr8.3:57416012-57416031 MS.gene88437:intron 40.0%
TAAAGTCAGCCAGGTTAAAC+TGG + chr8.3:57416915-57416934 None:intergenic 40.0%
TATGTGTTGTGCTGTCCATT+TGG - chr8.3:57416209-57416228 MS.gene88437:CDS 40.0%
TCTTTGTCCCATATCTGCAA+AGG + chr8.3:57416022-57416041 None:intergenic 40.0%
TTCAAGAACACATTCCAGTC+TGG - chr8.3:57415856-57415875 MS.gene88437:CDS 40.0%
TTGCAGATATGGGACAAAGA+AGG - chr8.3:57416022-57416041 MS.gene88437:intron 40.0%
! GGGGACTCTTTTTATAGTTG+TGG - chr8.3:57416319-57416338 MS.gene88437:intron 40.0%
!! GTTGTGCTTCTGAAATGGTT+AGG - chr8.3:57416177-57416196 MS.gene88437:CDS 40.0%
AGGTACAACTTGAACTCTGG+TGG + chr8.3:57417051-57417070 None:intergenic 45.0%
ATACAGCCTTGGGTATGATG+TGG - chr8.3:57415894-57415913 MS.gene88437:CDS 45.0%
ATGCCACTGAGAATGGATGA+GGG - chr8.3:57416877-57416896 MS.gene88437:intron 45.0%
ATGGAATTGATGCCAGGAGA+TGG - chr8.3:57417198-57417217 MS.gene88437:intron 45.0%
CAAGTTGTACCTTCCAATGC+AGG - chr8.3:57417059-57417078 MS.gene88437:intron 45.0%
CAGACATCTCATACCTGCAT+TGG + chr8.3:57417075-57417094 None:intergenic 45.0%
CATCTCATACCTGCATTGGA+AGG + chr8.3:57417071-57417090 None:intergenic 45.0%
CTTCTCTGATCGTCTCTACT+CGG - chr8.3:57417014-57417033 MS.gene88437:intron 45.0%
CTTTCTTGGTAAAGTCAGCC+AGG + chr8.3:57416924-57416943 None:intergenic 45.0%
GGAGCAAACCTTTGCAGATA+TGG - chr8.3:57416011-57416030 MS.gene88437:intron 45.0%
GGTACAACTTGAACTCTGGT+GGG + chr8.3:57417050-57417069 None:intergenic 45.0%
TATGCCACTGAGAATGGATG+AGG - chr8.3:57416876-57416895 MS.gene88437:intron 45.0%
TTATACCAAGTGTGGCAGCT+GGG + chr8.3:57416445-57416464 None:intergenic 45.0%
TTCTCAAGCGACAGTTTGCA+TGG + chr8.3:57416987-57417006 None:intergenic 45.0%
TTGCACTATGCCACTGAGAA+TGG - chr8.3:57416870-57416889 MS.gene88437:intron 45.0%
TTTATACCAAGTGTGGCAGC+TGG + chr8.3:57416446-57416465 None:intergenic 45.0%
! ACGTGCATCGACAGCAATTT+AGG + chr8.3:57416789-57416808 None:intergenic 45.0%
! ATGCAAACTGTCGCTTGAGA+AGG - chr8.3:57416986-57417005 MS.gene88437:intron 45.0%
! GGAAGGTACAACTTGAACTC+TGG + chr8.3:57417054-57417073 None:intergenic 45.0%
AGCCAACCACATCATACCCA+AGG + chr8.3:57415903-57415922 None:intergenic 50.0%
AGCCTTGGGTATGATGTGGT+TGG - chr8.3:57415898-57415917 MS.gene88437:CDS 50.0%
CAACCCTCATCCATTCTCAG+TGG + chr8.3:57416883-57416902 None:intergenic 50.0%
CACTGAGAATGGATGAGGGT+TGG - chr8.3:57416881-57416900 MS.gene88437:intron 50.0%
CATGGGCAGACTTACACACT+TGG + chr8.3:57416577-57416596 None:intergenic 50.0%
GTCCACATACATTACGTGCC+CGG - chr8.3:57416415-57416434 MS.gene88437:intron 50.0%
TGATCGTCTCTACTCGGAAG+AGG - chr8.3:57417020-57417039 MS.gene88437:intron 50.0%
TGGAGGATGGAATTGATGCC+AGG - chr8.3:57417192-57417211 MS.gene88437:intron 50.0%
TGTGGAGACACTACGAGTTC+AGG - chr8.3:57416960-57416979 MS.gene88437:intron 50.0%
AGCCGGGCACGTAATGTATG+TGG + chr8.3:57416420-57416439 None:intergenic 55.0%
AGTGTGGCAGCTGGGTCAGC+CGG + chr8.3:57416437-57416456 None:intergenic 65.0%
GCTGACCCAGCTGCCACACT+TGG - chr8.3:57416437-57416456 MS.gene88437:intron 65.0%
GTGTGGCAGCTGGGTCAGCC+GGG + chr8.3:57416436-57416455 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 57415853 57417262 57415853 ID=MS.gene88437
chr8.3 mRNA 57415853 57417262 57415853 ID=MS.gene88437.t1;Parent=MS.gene88437
chr8.3 exon 57417210 57417262 57417210 ID=MS.gene88437.t1.exon1;Parent=MS.gene88437.t1
chr8.3 CDS 57417210 57417262 57417210 ID=cds.MS.gene88437.t1;Parent=MS.gene88437.t1
chr8.3 exon 57417072 57417103 57417072 ID=MS.gene88437.t1.exon2;Parent=MS.gene88437.t1
chr8.3 CDS 57417072 57417103 57417072 ID=cds.MS.gene88437.t1;Parent=MS.gene88437.t1
chr8.3 exon 57416538 57416779 57416538 ID=MS.gene88437.t1.exon3;Parent=MS.gene88437.t1
chr8.3 CDS 57416538 57416779 57416538 ID=cds.MS.gene88437.t1;Parent=MS.gene88437.t1
chr8.3 exon 57416035 57416271 57416035 ID=MS.gene88437.t1.exon4;Parent=MS.gene88437.t1
chr8.3 CDS 57416035 57416271 57416035 ID=cds.MS.gene88437.t1;Parent=MS.gene88437.t1
chr8.3 exon 57415853 57415969 57415853 ID=MS.gene88437.t1.exon5;Parent=MS.gene88437.t1
chr8.3 CDS 57415853 57415969 57415853 ID=cds.MS.gene88437.t1;Parent=MS.gene88437.t1
Gene Sequence

>MS.gene88437

ATGTTCAAGAACACATTCCAGTCTGGATTTCTTTCCATATTATACAGCCTTGGGTATGATGTGGTTGGCTTGTTTATTTCATATTTCAGTATCATATGTTTGTTGTGATTAAGGTGCTGAAATTTTGCATTTTGTTTTCAATTTTTTTTATTATTGTAGGAGCAAACCTTTGCAGATATGGGACAAAGAAGGTAATTTTTGTTTTGTTAGCAGTGACATTAGATGGTCATTTTTCTGCTTGTTAGAAATTTGATATGCTGAATTGAATTGATCCGAAATCATTTTACTTTGATTATTTTATTCTCGTTGTTGAAGTTTTGTTTAGTTGTGCTTCTGAAATGGTTAGGCTGCTGCCTTATGTGTTGTGCTGTCCATTTGGCCATGTTATGAAAGGCTGATGTGTAAATATGTTGAAGAGTGACTGAGGGATGAAATAACAATTTTTTTAAACTTACGATTTTGTCTTGGGGACTCTTTTTATAGTTGTGGATGGCCATATTAAGCGACCACAAGATGAAGACATACAATCTAATGTACTGGAAATAATTGGATCGAATATTCAGTCCACATACATTACGTGCCCGGCTGACCCAGCTGCCACACTTGGTATAAAACTTCCATTCTTGGTTATGATTGTGAAGAATCTAAAGAAGTACTTCACATTTGAGATTCAAGTTTTGGATGATAAGAATGTCAGACGACGATTTCGAGCTTCTAATTTCCAAGTGTGTAAGTCTGCCCATGTTTGTGTGTCTTATAGGATTGCCAATCTTGCAGATGTTTAATTTCTTTCATATATATTTTGTGCTGTTCTTTCTTCCATTTAATTATATGGAGAACTTTGATTTTCTGACTAGTAACGCATTTGCATGATTCTTTCTTACTGAAATAAACTATTTATATAGAATCACAAATACAGTGATGCTATTTCTACCTAAATTGCTGTCGATGCACGTTACAAGTGATTTGTTTTATTATTTTTATGTCATAGGCTGTCACTAGAGTAAAGCCGTACATTTGCACTATGCCACTGAGAATGGATGAGGGTTGGAATCAAATCCAGTTTAACCTGGCTGACTTTACCAAGAAAGCATATGGGACTAATTATGTGGAGACACTACGAGTTCAGGTCCATGCAAACTGTCGCTTGAGAAGGATATACTTCTCTGATCGTCTCTACTCGGAAGAGGAACTCCCACCAGAGTTCAAGTTGTACCTTCCAATGCAGGTATGAGATGTCTGCATATGAACAGCTTGTTTTTTGTTTATCTTTTCATCTCAATTGCATGTCAGGTTTCACCTTGTATTGTTCATCATGCAGAAATAATGAAAATGTCATTGGAGGATGGAATTGATGCCAGGAGATGGTTGAATGTTATGTGTAGTTGTGTGTCGGCTTCCCTGCACTAA

Protein sequence

>MS.gene88437.t1

MFKNTFQSGFLSILYSLGSKPLQIWDKEVVDGHIKRPQDEDIQSNVLEIIGSNIQSTYITCPADPAATLGIKLPFLVMIVKNLKKYFTFEIQVLDDKNVRRRFRASNFQAVTRVKPYICTMPLRMDEGWNQIQFNLADFTKKAYGTNYVETLRVQVHANCRLRRIYFSDRLYSEEELPPEFKLYLPMQVSPCIVHHAEIMKMSLEDGIDARRWLNVMCSCVSASLH