AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92650


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92650.t1 MTR_3g030055 86.928 153 15 2 1 153 8 155 1.15e-92 265
MS.gene92650.t1 MTR_3g030055 88.776 98 9 1 1 98 1 96 3.32e-58 177
MS.gene92650.t1 MTR_3g034200 46.795 156 72 4 6 151 2 156 3.88e-34 118
MS.gene92650.t1 MTR_3g050860 38.217 157 86 6 1 151 1 152 1.70e-17 78.2
MS.gene92650.t1 MTR_0439s0020 34.459 148 86 7 1 142 1 143 2.01e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92650.t1 AT5G13780 43.871 155 76 4 6 150 2 155 5.90e-30 107
MS.gene92650.t1 AT1G03150 28.070 114 78 3 35 146 39 150 1.72e-11 59.3

Find 38 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCACAATGTGCCAATATT+CGG 0.287513 3.3:+33732521 None:intergenic
CTCGTTACTCGCCCGAATAT+TGG 0.335099 3.3:-33732533 MS.gene92650:CDS
TGCATAAGAGTTGCAACATT+TGG 0.337938 3.3:-33732587 MS.gene92650:CDS
TCCACAATGTGCCAATATTC+GGG 0.349965 3.3:+33732522 None:intergenic
TCTTGGTTTGTTTCAAAAGA+TGG 0.379062 3.3:-33731610 MS.gene92650:CDS
TGCAACATTTGGAGACGTAT+TGG 0.394327 3.3:-33732576 MS.gene92650:CDS
GATAGGTATGGTGAACGAAT+AGG 0.421395 3.3:+33732393 None:intergenic
CTCCTCATCATCAAAATACT+TGG 0.430558 3.3:+33731563 None:intergenic
ATAGGTATGGTGAACGAATA+GGG 0.433258 3.3:+33732394 None:intergenic
CTCTGAAGGTGAAGAGGAAG+AGG 0.447607 3.3:-33732444 MS.gene92650:CDS
CTTGGTTTGTTTCAAAAGAT+GGG 0.449922 3.3:-33731609 MS.gene92650:CDS
CGCCCAAAACACCATGGAAA+AGG 0.453353 3.3:-33732336 MS.gene92650:intron
AAGACGTTTCAGTGCATGTT+CGG 0.461363 3.3:-33731650 MS.gene92650:CDS
AAGAGCAACTCTGCAGCTCT+TGG 0.462439 3.3:-33731627 MS.gene92650:CDS
CATTTGGAGACGTATTGGAT+TGG 0.499964 3.3:-33732571 MS.gene92650:CDS
AGGAACAATGTCTTCAAAGA+AGG 0.508100 3.3:-33732612 None:intergenic
GTTGCTTACCTCCTCTCTGA+AGG 0.508220 3.3:-33732458 MS.gene92650:CDS
AAGGTGAAGAGGAAGAGGAA+GGG 0.530803 3.3:-33732439 MS.gene92650:CDS
GTCTTGAGGAAGATACTCAA+GGG 0.554037 3.3:-33731531 MS.gene92650:CDS
GAAGGTGAAGAGGAAGAGGA+AGG 0.556577 3.3:-33732440 MS.gene92650:CDS
TGTCTTGAGGAAGATACTCA+AGG 0.562517 3.3:-33731532 MS.gene92650:CDS
CCTCCTCTCTGAAGGTGAAG+AGG 0.564442 3.3:-33732450 MS.gene92650:CDS
GCCCGAATATTGGCACATTG+TGG 0.568169 3.3:-33732523 MS.gene92650:CDS
TTACACACTTCACGAGGTGA+AGG 0.607853 3.3:-33731586 MS.gene92650:CDS
CCTCTTCACCTTCAGAGAGG+AGG 0.614706 3.3:+33732450 None:intergenic
AGGAGGACGCTATTGTCTTG+AGG 0.615172 3.3:-33731545 MS.gene92650:CDS
CACCGCCGCCCAAAACACCA+TGG 0.620119 3.3:-33732342 MS.gene92650:CDS
ATAGGGAGGCGATACTAAAG+TGG 0.620673 3.3:+33732411 None:intergenic
CACCATACCTATCGTAAACG+TGG 0.631082 3.3:-33732383 MS.gene92650:CDS
GAGTGGGTCTCACAGTTAGG+TGG 0.634645 3.3:+33731497 None:intergenic
CTTCCTCTTCACCTTCAGAG+AGG 0.638002 3.3:+33732447 None:intergenic
GAAGAAGCTTATTCATGTCG+CGG 0.645766 3.3:-33732501 MS.gene92650:CDS
TACCACGTTTACGATAGGTA+TGG 0.651983 3.3:+33732381 None:intergenic
AGCGATACCACGTTTACGAT+AGG 0.681782 3.3:+33732376 None:intergenic
TCTTGAGGAAGATACTCAAG+GGG 0.688967 3.3:-33731530 MS.gene92650:CDS
CATGTCGCGGACAATTGCAG+CGG 0.697708 3.3:-33732488 MS.gene92650:CDS
GATGGGTTACACACTTCACG+AGG 0.713948 3.3:-33731592 MS.gene92650:CDS
GGTATGGTGAACGAATAGGG+AGG 0.789435 3.3:+33732397 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTGTTTTTTGGATT+AGG - chr3.3:33731830-33731849 MS.gene92650:intron 15.0%
!!! TCTGTTATAATTTTGTTTTT+TGG - chr3.3:33731824-33731843 MS.gene92650:intron 15.0%
!!! AGACTTTTAATGGAAAAAAT+AGG + chr3.3:33732412-33732431 None:intergenic 20.0%
!!! TTTCTTCTTGTTAATGATTT+TGG - chr3.3:33732204-33732223 MS.gene92650:intron 20.0%
! CAACCAAAAACATCAAAAAA+AGG + chr3.3:33732383-33732402 None:intergenic 25.0%
! GAAAACCAAAAGAAAAATCT+CGG + chr3.3:33732288-33732307 None:intergenic 25.0%
!! ACATTGTAACAGACTTTTAA+TGG + chr3.3:33732422-33732441 None:intergenic 25.0%
!! ATGGAGTTTGTTTAATGTTT+AGG - chr3.3:33731910-33731929 MS.gene92650:intron 25.0%
!!! AGACCTTTTTTTGATGTTTT+TGG - chr3.3:33732377-33732396 MS.gene92650:CDS 25.0%
!!! TTTTCTTGTTTCCTAATCTT+GGG - chr3.3:33732303-33732322 MS.gene92650:intron 25.0%
ACAATGTGTAATTTGACTGA+AGG - chr3.3:33732435-33732454 MS.gene92650:CDS 30.0%
AGAAAACAGAAAATCATCAC+GGG + chr3.3:33732033-33732052 None:intergenic 30.0%
CAGAAAACAGAAAATCATCA+CGG + chr3.3:33732034-33732053 None:intergenic 30.0%
TAATGTTTAGGTATGAAGCT+TGG - chr3.3:33731922-33731941 MS.gene92650:intron 30.0%
TCCATACATAACCAGTTAAA+AGG + chr3.3:33731895-33731914 None:intergenic 30.0%
! CTTCTTGTTAATGATTTTGG+TGG - chr3.3:33732207-33732226 MS.gene92650:intron 30.0%
! TTTCTTGTTTCCTAATCTTG+GGG - chr3.3:33732304-33732323 MS.gene92650:intron 30.0%
!! CTTGGTTTGTTTCAAAAGAT+GGG - chr3.3:33732508-33732527 MS.gene92650:CDS 30.0%
!! GTTTTCTTGTTTCCTAATCT+TGG - chr3.3:33732302-33732321 MS.gene92650:intron 30.0%
!! TCTTGGTTTGTTTCAAAAGA+TGG - chr3.3:33732507-33732526 MS.gene92650:CDS 30.0%
!! TTGTTAATGATTTTGGTGGT+TGG - chr3.3:33732211-33732230 MS.gene92650:intron 30.0%
AAAACACCATGGAAAAGGTT+AGG - chr3.3:33731786-33731805 MS.gene92650:intron 35.0%
AACCAAAAGAAAAATCTCGG+AGG + chr3.3:33732285-33732304 None:intergenic 35.0%
AATCTGAGTTTCACTATGCT+AGG - chr3.3:33732058-33732077 MS.gene92650:intron 35.0%
ATAGGTATGGTGAACGAATA+GGG + chr3.3:33731726-33731745 None:intergenic 35.0%
ATGTGTAATTTGACTGAAGG+TGG - chr3.3:33732438-33732457 MS.gene92650:CDS 35.0%
CTAGGATTATCTTCTATTCC+TGG - chr3.3:33732076-33732095 MS.gene92650:intron 35.0%
CTCCTCATCATCAAAATACT+TGG + chr3.3:33732557-33732576 None:intergenic 35.0%
TGCATAAGAGTTGCAACATT+TGG - chr3.3:33731530-33731549 MS.gene92650:CDS 35.0%
TGTGTAATTTGACTGAAGGT+GGG - chr3.3:33732439-33732458 MS.gene92650:CDS 35.0%
TTATCTTCTATTCCTGGAAG+TGG - chr3.3:33732082-33732101 MS.gene92650:intron 35.0%
TTCCACAATGTGCCAATATT+CGG + chr3.3:33731599-33731618 None:intergenic 35.0%
! AACAAACCTAACCTTTTCCA+TGG + chr3.3:33731795-33731814 None:intergenic 35.0%
! AACCTTTTCCATGGTGTTTT+GGG + chr3.3:33731786-33731805 None:intergenic 35.0%
! CGTGATGATTTTCTGTTTTC+TGG - chr3.3:33732032-33732051 MS.gene92650:intron 35.0%
! TAACCTTTTCCATGGTGTTT+TGG + chr3.3:33731787-33731806 None:intergenic 35.0%
! TTGGTTGAGTTGTTTCGTTA+GGG - chr3.3:33731941-33731960 MS.gene92650:intron 35.0%
! TTTTCAGATAGATCACACAG+TGG + chr3.3:33732010-33732029 None:intergenic 35.0%
!! GCCTTTTAACTGGTTATGTA+TGG - chr3.3:33731891-33731910 MS.gene92650:intron 35.0%
!! TTCTGTAGTTAGTCTTGTCT+GGG - chr3.3:33732247-33732266 MS.gene92650:intron 35.0%
!! TTTCTGTAGTTAGTCTTGTC+TGG - chr3.3:33732246-33732265 MS.gene92650:intron 35.0%
!!! CAAGTATTTTGATGATGAGG+AGG - chr3.3:33732555-33732574 MS.gene92650:CDS 35.0%
AAACACTTCCCACAGAGAAT+TGG + chr3.3:33732162-33732181 None:intergenic 40.0%
AAAGGGAGTGGCAATGATTA+GGG + chr3.3:33732122-33732141 None:intergenic 40.0%
AAGACGTTTCAGTGCATGTT+CGG - chr3.3:33732467-33732486 MS.gene92650:CDS 40.0%
CATTTGGAGACGTATTGGAT+TGG - chr3.3:33731546-33731565 MS.gene92650:CDS 40.0%
GAAGAAGCTTATTCATGTCG+CGG - chr3.3:33731616-33731635 MS.gene92650:CDS 40.0%
GATAGGTATGGTGAACGAAT+AGG + chr3.3:33731727-33731746 None:intergenic 40.0%
GTCTTGAGGAAGATACTCAA+GGG - chr3.3:33732586-33732605 MS.gene92650:CDS 40.0%
TAAAGGGAGTGGCAATGATT+AGG + chr3.3:33732123-33732142 None:intergenic 40.0%
TACCACGTTTACGATAGGTA+TGG + chr3.3:33731739-33731758 None:intergenic 40.0%
TATTCCTGGAAGTGGTTCTT+CGG - chr3.3:33732090-33732109 MS.gene92650:intron 40.0%
TCCACAATGTGCCAATATTC+GGG + chr3.3:33731598-33731617 None:intergenic 40.0%
TCTTGAGGAAGATACTCAAG+GGG - chr3.3:33732587-33732606 MS.gene92650:CDS 40.0%
TGCAACATTTGGAGACGTAT+TGG - chr3.3:33731541-33731560 MS.gene92650:CDS 40.0%
TGTCTTGAGGAAGATACTCA+AGG - chr3.3:33732585-33732604 MS.gene92650:CDS 40.0%
! CTTGGTTGAGTTGTTTCGTT+AGG - chr3.3:33731940-33731959 MS.gene92650:intron 40.0%
! GGATTAGGTTTGTCACTTGT+GGG - chr3.3:33731845-33731864 MS.gene92650:intron 40.0%
! GTCCTCCGAGATTTTTCTTT+TGG - chr3.3:33732280-33732299 MS.gene92650:intron 40.0%
! TGGATTAGGTTTGTCACTTG+TGG - chr3.3:33731844-33731863 MS.gene92650:intron 40.0%
! TGGTTGAGTTGTTTCGTTAG+GGG - chr3.3:33731942-33731961 MS.gene92650:intron 40.0%
! TGTGGTATAGGCCTTTTAAC+TGG - chr3.3:33731881-33731900 MS.gene92650:intron 40.0%
!! TCTGTAGTTAGTCTTGTCTG+GGG - chr3.3:33732248-33732267 MS.gene92650:intron 40.0%
!!! GGCCAAGTATTTTGATGATG+AGG - chr3.3:33732552-33732571 MS.gene92650:CDS 40.0%
!!! TTCTCTGTGGGAAGTGTTTT+TGG - chr3.3:33732163-33732182 MS.gene92650:intron 40.0%
AAAATCCGCACCCCAAGATT+AGG + chr3.3:33732317-33732336 None:intergenic 45.0%
AAGGTGAAGAGGAAGAGGAA+GGG - chr3.3:33731678-33731697 MS.gene92650:intron 45.0%
AGCGATACCACGTTTACGAT+AGG + chr3.3:33731744-33731763 None:intergenic 45.0%
AGGCGAAACCAATTCTCTGT+GGG - chr3.3:33732151-33732170 MS.gene92650:intron 45.0%
ATAGGGAGGCGATACTAAAG+TGG + chr3.3:33731709-33731728 None:intergenic 45.0%
ATTGCCACTCCCTTTATGAG+TGG - chr3.3:33732127-33732146 MS.gene92650:intron 45.0%
CACCATACCTATCGTAAACG+TGG - chr3.3:33731734-33731753 MS.gene92650:intron 45.0%
TAGCGTATAGGGTGTGGTAT+AGG - chr3.3:33731869-33731888 MS.gene92650:intron 45.0%
TGTTTCCTAATCTTGGGGTG+CGG - chr3.3:33732309-33732328 MS.gene92650:intron 45.0%
TTACACACTTCACGAGGTGA+AGG - chr3.3:33732531-33732550 MS.gene92650:CDS 45.0%
TTGCCACTCCCTTTATGAGT+GGG - chr3.3:33732128-33732147 MS.gene92650:intron 45.0%
TTTCGCCTCCCACTCATAAA+GGG + chr3.3:33732139-33732158 None:intergenic 45.0%
! TGAGTTGTTTCGTTAGGGGT+GGG - chr3.3:33731946-33731965 MS.gene92650:intron 45.0%
! TTGAGTTGTTTCGTTAGGGG+TGG - chr3.3:33731945-33731964 MS.gene92650:intron 45.0%
!! CACTTGTGGGTTAGCGTATA+GGG - chr3.3:33731858-33731877 MS.gene92650:intron 45.0%
!! CTTTTCCATGGTGTTTTGGG+CGG + chr3.3:33731783-33731802 None:intergenic 45.0%
!! TCACTTGTGGGTTAGCGTAT+AGG - chr3.3:33731857-33731876 MS.gene92650:intron 45.0%
AAGAGCAACTCTGCAGCTCT+TGG - chr3.3:33732490-33732509 MS.gene92650:CDS 50.0%
AAGGTCTGTACAACAGCTGC+CGG + chr3.3:33732364-33732383 None:intergenic 50.0%
AGGAGGACGCTATTGTCTTG+AGG - chr3.3:33732572-33732591 MS.gene92650:CDS 50.0%
CGCCCAAAACACCATGGAAA+AGG - chr3.3:33731781-33731800 MS.gene92650:intron 50.0%
CTCGTTACTCGCCCGAATAT+TGG - chr3.3:33731584-33731603 MS.gene92650:CDS 50.0%
CTTCCTCTTCACCTTCAGAG+AGG + chr3.3:33731673-33731692 None:intergenic 50.0%
GAAGGTGAAGAGGAAGAGGA+AGG - chr3.3:33731677-33731696 MS.gene92650:intron 50.0%
GAGGCGAAACCAATTCTCTG+TGG - chr3.3:33732150-33732169 MS.gene92650:intron 50.0%
GCCCGAATATTGGCACATTG+TGG - chr3.3:33731594-33731613 MS.gene92650:CDS 50.0%
GGTATGGTGAACGAATAGGG+AGG + chr3.3:33731723-33731742 None:intergenic 50.0%
GGTTCTCTCTACCTTGAGAC+CGG - chr3.3:33732342-33732361 MS.gene92650:CDS 50.0%
GTTGCTTACCTCCTCTCTGA+AGG - chr3.3:33731659-33731678 MS.gene92650:CDS 50.0%
GTTTCGCCTCCCACTCATAA+AGG + chr3.3:33732140-33732159 None:intergenic 50.0%
TTCACCGAAGAACCACTTCC+AGG + chr3.3:33732097-33732116 None:intergenic 50.0%
TTGTTTCGTTAGGGGTGGGA+AGG - chr3.3:33731950-33731969 MS.gene92650:intron 50.0%
! CTCTGAAGGTGAAGAGGAAG+AGG - chr3.3:33731673-33731692 MS.gene92650:intron 50.0%
! TTGGGGTGCGGATTTTCTCT+AGG - chr3.3:33732321-33732340 MS.gene92650:intron 50.0%
!! GATGGGTTACACACTTCACG+AGG - chr3.3:33732525-33732544 MS.gene92650:CDS 50.0%
CATGTCGCGGACAATTGCAG+CGG - chr3.3:33731629-33731648 MS.gene92650:CDS 55.0%
CCACTCCCTTTATGAGTGGG+AGG - chr3.3:33732131-33732150 MS.gene92650:intron 55.0%
CCTCCCACTCATAAAGGGAG+TGG + chr3.3:33732134-33732153 None:intergenic 55.0%
CCTCTTCACCTTCAGAGAGG+AGG + chr3.3:33731670-33731689 None:intergenic 55.0%
TACAACAGCTGCCGGTCTCA+AGG + chr3.3:33732356-33732375 None:intergenic 55.0%
! CCTCCTCTCTGAAGGTGAAG+AGG - chr3.3:33731667-33731686 MS.gene92650:intron 55.0%
!! GTCTTGTCTGGGGAGTGTTC+TGG - chr3.3:33732258-33732277 MS.gene92650:intron 55.0%
!! GTGGGTTAGCGTATAGGGTG+TGG - chr3.3:33731863-33731882 MS.gene92650:intron 55.0%
!! TTCCATGGTGTTTTGGGCGG+CGG + chr3.3:33731780-33731799 None:intergenic 55.0%
GTGGGAAGGCAGAGTAGGCT+AGG - chr3.3:33731964-33731983 MS.gene92650:intron 60.0%
TAGGGGTGGGAAGGCAGAGT+AGG - chr3.3:33731959-33731978 MS.gene92650:intron 60.0%
CACCGCCGCCCAAAACACCA+TGG - chr3.3:33731775-33731794 MS.gene92650:intron 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 33731512 33732627 33731512 ID=MS.gene92650
chr3.3 mRNA 33731512 33732627 33731512 ID=MS.gene92650.t1;Parent=MS.gene92650
chr3.3 exon 33732337 33732627 33732337 ID=MS.gene92650.t1.exon1;Parent=MS.gene92650.t1
chr3.3 CDS 33732337 33732627 33732337 ID=cds.MS.gene92650.t1;Parent=MS.gene92650.t1
chr3.3 exon 33731512 33731682 33731512 ID=MS.gene92650.t1.exon2;Parent=MS.gene92650.t1
chr3.3 CDS 33731512 33731682 33731512 ID=cds.MS.gene92650.t1;Parent=MS.gene92650.t1
Gene Sequence

>MS.gene92650

ATGTCTTCAAAGAAGGTGTGCATAAGAGTTGCAACATTTGGAGACGTATTGGATTGGAACAGTAACAACAATCTCGTTACTCGCCCGAATATTGGCACATTGTGGAAGAAGCTTATTCATGTCGCGGACAATTGCAGCGGCTGTATTGTTGCTTACCTCCTCTCTGAAGGTGAAGAGGAAGAGGAAGGGAATGACCACTTTAGTATCGCCTCCCTATTCGTTCACCATACCTATCGTAAACGTGGTATCGCTACTGATCTTATCACCGCCGCCCAAAACACCATGGAAAAGGTTAGGTTTGTTTCTTTATATTCTGTTATAATTTTGTTTTTTGGATTAGGTTTGTCACTTGTGGGTTAGCGTATAGGGTGTGGTATAGGCCTTTTAACTGGTTATGTATGGAGTTTGTTTAATGTTTAGGTATGAAGCTTGGTTGAGTTGTTTCGTTAGGGGTGGGAAGGCAGAGTAGGCTAGGTTTTTTGTTAATCTAGCACGCCACTGTGTGATCTATCTGAAAACCCGTGATGATTTTCTGTTTTCTGGTAGAATCTGAGTTTCACTATGCTAGGATTATCTTCTATTCCTGGAAGTGGTTCTTCGGTGAATTCCCTAATCATTGCCACTCCCTTTATGAGTGGGAGGCGAAACCAATTCTCTGTGGGAAGTGTTTTTGGCTTCTTTTCTTCTTTCTCTTTCTTCTTGTTAATGATTTTGGTGGTTGGTGTTTGTTGTTTTTTCTGTAGTTAGTCTTGTCTGGGGAGTGTTCTGGTCCTCCGAGATTTTTCTTTTGGTTTTCTTGTTTCCTAATCTTGGGGTGCGGATTTTCTCTAGGTTCTCTCTACCTTGAGACCGGCAGCTGTTGTACAGACCTTTTTTTGATGTTTTTGGTTGTCAATACCTATTTTTTCCATTAAAAGTCTGTTACAATGTGTAATTTGACTGAAGGTGGGTGCTAAAGACGTTTCAGTGCATGTTCGGAAGAGCAACTCTGCAGCTCTTGGTTTGTTTCAAAAGATGGGTTACACACTTCACGAGGTGAAGGCCAAGTATTTTGATGATGAGGAGGACGCTATTGTCTTGAGGAAGATACTCAAGGGGAAGCATGTTGCCACCTAA

Protein sequence

>MS.gene92650.t1

MSSKKVCIRVATFGDVLDWNSNNNLVTRPNIGTLWKKLIHVADNCSGCIVAYLLSEGEEEEEGNDHFSIASLFVHHTYRKRGIATDLITAAQNTMEKVGAKDVSVHVRKSNSAALGLFQKMGYTLHEVKAKYFDDEEDAIVLRKILKGKHVAT