AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95934


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95934.t1 MTR_3g030055 88.235 153 13 2 1 153 8 155 2.94e-94 270
MS.gene95934.t1 MTR_3g030055 89.796 98 8 1 1 98 1 96 2.48e-59 180
MS.gene95934.t1 MTR_3g034200 48.408 157 68 5 6 151 2 156 1.93e-35 121
MS.gene95934.t1 MTR_3g050860 39.490 157 84 6 1 151 1 152 4.48e-19 82.4
MS.gene95934.t1 MTR_0439s0020 35.135 148 85 7 1 142 1 143 1.32e-13 64.3
MS.gene95934.t1 MTR_7g117040 28.070 114 78 3 35 146 39 150 6.32e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95934.t1 AT5G13780 45.513 156 72 5 6 150 2 155 2.63e-31 111
MS.gene95934.t1 AT1G03150 28.947 114 77 3 35 146 39 150 3.64e-12 61.2

Find 40 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCACAATGTGCCAATATT+CGG 0.287513 3.2:+33977095 None:intergenic
CTCGTTACTCGCCCGAATAT+TGG 0.335099 3.2:-33977107 MS.gene95934:CDS
TGCATAAGAGTTGCAACATT+TGG 0.337938 3.2:-33977161 MS.gene95934:CDS
GAGCTGCAGAGTTGCTCTTC+CGG 0.345662 3.2:+33976220 None:intergenic
TCCACAATGTGCCAATATTC+GGG 0.349965 3.2:+33977096 None:intergenic
TCTTGGTTTGTTTGAAAAGA+TGG 0.363394 3.2:-33976199 MS.gene95934:CDS
TGCAACATTTGGAGACGTAT+TGG 0.394327 3.2:-33977150 MS.gene95934:CDS
GATAGGTATGGTGAACGAAT+AGG 0.421395 3.2:+33976967 None:intergenic
CTCCTCATCATCAAAATACT+TGG 0.430558 3.2:+33976152 None:intergenic
ATAGGTATGGTGAACGAATA+GGG 0.433258 3.2:+33976968 None:intergenic
CTCTGAAGGTGAAGAGGAAG+AGG 0.447607 3.2:-33977018 MS.gene95934:CDS
CGCCCAAAACACCATGGAAA+AGG 0.453353 3.2:-33976910 MS.gene95934:intron
CTTGGTTTGTTTGAAAAGAT+GGG 0.459057 3.2:-33976198 MS.gene95934:CDS
AAGAGCAACTCTGCAGCTCT+TGG 0.462439 3.2:-33976216 MS.gene95934:CDS
AAGACGTCTCAGTGCACGTC+CGG 0.493597 3.2:-33976239 MS.gene95934:CDS
CATTTGGAGACGTATTGGAT+TGG 0.499964 3.2:-33977145 MS.gene95934:CDS
AGGAACAATGTCTTCAAAGA+AGG 0.508100 3.2:-33977186 None:intergenic
GTTGCTTACCTCCTCTCTGA+AGG 0.508220 3.2:-33977032 MS.gene95934:CDS
AAGGTGAAGAGGAAGAGGAA+GGG 0.530803 3.2:-33977013 MS.gene95934:CDS
GTCTTGAGGAAGATACTCAA+GGG 0.554037 3.2:-33976120 MS.gene95934:CDS
GAAGGTGAAGAGGAAGAGGA+AGG 0.556577 3.2:-33977014 MS.gene95934:CDS
TGTCTTGAGGAAGATACTCA+AGG 0.562517 3.2:-33976121 MS.gene95934:CDS
CCTCCTCTCTGAAGGTGAAG+AGG 0.564442 3.2:-33977024 MS.gene95934:CDS
GCCCGAATATTGGCACATTG+TGG 0.568169 3.2:-33977097 MS.gene95934:CDS
GAAGAGGAAGAGGAAGGGAA+TGG 0.582705 3.2:-33977008 MS.gene95934:CDS
TTACACACTTCACGAGGTGA+AGG 0.607853 3.2:-33976175 MS.gene95934:CDS
ATAGGGAGGCGATACTAAAG+TGG 0.612204 3.2:+33976985 None:intergenic
CCTCTTCACCTTCAGAGAGG+AGG 0.614706 3.2:+33977024 None:intergenic
AGGAGGACGCTATTGTCTTG+AGG 0.615172 3.2:-33976134 MS.gene95934:CDS
CACCGCCGCCCAAAACACCA+TGG 0.620119 3.2:-33976916 MS.gene95934:CDS
CACCATACCTATCGTAAACG+TGG 0.631082 3.2:-33976957 MS.gene95934:CDS
GAGTGGGTCTCACAGTTAGG+TGG 0.634645 3.2:+33976086 None:intergenic
CTTCCTCTTCACCTTCAGAG+AGG 0.638002 3.2:+33977021 None:intergenic
GAAGAAGCTTATTCATGTCG+CGG 0.645766 3.2:-33977075 MS.gene95934:CDS
TACCACGTTTACGATAGGTA+TGG 0.651983 3.2:+33976955 None:intergenic
AGCGATACCACGTTTACGAT+AGG 0.681782 3.2:+33976950 None:intergenic
TCTTGAGGAAGATACTCAAG+GGG 0.688967 3.2:-33976119 MS.gene95934:CDS
CATGTCGCGGACAATTGCAG+CGG 0.697708 3.2:-33977062 MS.gene95934:CDS
GATGGGTTACACACTTCACG+AGG 0.713948 3.2:-33976181 MS.gene95934:CDS
GGTATGGTGAACGAATAGGG+AGG 0.789435 3.2:+33976971 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTGTTTTTTGGATT+AGG - chr3.2:33976419-33976438 MS.gene95934:intron 15.0%
!!! TCTGTTATAATTTTGTTTTT+TGG - chr3.2:33976413-33976432 MS.gene95934:intron 15.0%
! ACAACCAAAAACATCAAAAA+AGG + chr3.2:33976958-33976977 None:intergenic 25.0%
! GAAAACCAAAAGAAAAATCT+CGG + chr3.2:33976863-33976882 None:intergenic 25.0%
!! ACATTGTAACAGACTTTTAA+TGG + chr3.2:33976996-33977015 None:intergenic 25.0%
!! ATGGAGTTTGTTTAATGTTT+AGG - chr3.2:33976499-33976518 MS.gene95934:intron 25.0%
!!! TTTTCTTGTTTCCTAATCTT+GGG - chr3.2:33976878-33976897 MS.gene95934:intron 25.0%
ACAATGTGTAATTTGACTGA+AGG - chr3.2:33977009-33977028 MS.gene95934:CDS 30.0%
AGAAAACAGAAAATCATCAC+GGG + chr3.2:33976622-33976641 None:intergenic 30.0%
CAGAAAACAGAAAATCATCA+CGG + chr3.2:33976623-33976642 None:intergenic 30.0%
TAATGTTTAGGTATGAAGCT+TGG - chr3.2:33976511-33976530 MS.gene95934:intron 30.0%
TCCATACATAACCAGTTAAA+AGG + chr3.2:33976484-33976503 None:intergenic 30.0%
! TTTCTTCTTGTTAGTGCTTT+TGG - chr3.2:33976779-33976798 MS.gene95934:intron 30.0%
! TTTCTTGTTTCCTAATCTTG+GGG - chr3.2:33976879-33976898 MS.gene95934:intron 30.0%
!! CTTGGTTTGTTTGAAAAGAT+GGG - chr3.2:33977082-33977101 MS.gene95934:CDS 30.0%
!! GTTTTCTTGTTTCCTAATCT+TGG - chr3.2:33976877-33976896 MS.gene95934:intron 30.0%
!! TCTTGGTTTGTTTGAAAAGA+TGG - chr3.2:33977081-33977100 MS.gene95934:CDS 30.0%
!!! CAGACCTTTTTTGATGTTTT+TGG - chr3.2:33976951-33976970 MS.gene95934:CDS 30.0%
AAAACACCATGGAAAAGGTT+AGG - chr3.2:33976375-33976394 MS.gene95934:intron 35.0%
AACCAAAAGAAAAATCTCGG+AGG + chr3.2:33976860-33976879 None:intergenic 35.0%
AATCTGAGTTTCACTATGCT+AGG - chr3.2:33976647-33976666 MS.gene95934:intron 35.0%
ATAGGTATGGTGAACGAATA+GGG + chr3.2:33976315-33976334 None:intergenic 35.0%
ATGTGTAATTTGACTGAAGG+TGG - chr3.2:33977012-33977031 MS.gene95934:CDS 35.0%
CTAGGATTATCTTCTATTCC+GGG - chr3.2:33976665-33976684 MS.gene95934:intron 35.0%
CTCCTCATCATCAAAATACT+TGG + chr3.2:33977131-33977150 None:intergenic 35.0%
GCTAGGATTATCTTCTATTC+CGG - chr3.2:33976664-33976683 MS.gene95934:intron 35.0%
TGCATAAGAGTTGCAACATT+TGG - chr3.2:33976119-33976138 MS.gene95934:CDS 35.0%
TGTGTAATTTGACTGAAGGT+GGG - chr3.2:33977013-33977032 MS.gene95934:CDS 35.0%
TTCCACAATGTGCCAATATT+CGG + chr3.2:33976188-33976207 None:intergenic 35.0%
! AACAAACCTAACCTTTTCCA+TGG + chr3.2:33976384-33976403 None:intergenic 35.0%
! AACCTTTTCCATGGTGTTTT+GGG + chr3.2:33976375-33976394 None:intergenic 35.0%
! CGTGATGATTTTCTGTTTTC+TGG - chr3.2:33976621-33976640 MS.gene95934:intron 35.0%
! TAACCTTTTCCATGGTGTTT+TGG + chr3.2:33976376-33976395 None:intergenic 35.0%
! TTGGTTGAGTTGTTTCGTTA+GGG - chr3.2:33976530-33976549 MS.gene95934:intron 35.0%
! TTTTCAGATAGATCACACAG+TGG + chr3.2:33976599-33976618 None:intergenic 35.0%
!! GCCTTTTAACTGGTTATGTA+TGG - chr3.2:33976480-33976499 MS.gene95934:intron 35.0%
!! TCTGTAGTTAGTCTTGTCTA+GGG - chr3.2:33976823-33976842 MS.gene95934:intron 35.0%
!! TTCTGTAGTTAGTCTTGTCT+AGG - chr3.2:33976822-33976841 MS.gene95934:intron 35.0%
!!! CAAGTATTTTGATGATGAGG+AGG - chr3.2:33977129-33977148 MS.gene95934:CDS 35.0%
AAACACTTCCCACAGAGAAT+TGG + chr3.2:33976751-33976770 None:intergenic 40.0%
AAAGGGAGTGGCAATGATTA+GGG + chr3.2:33976711-33976730 None:intergenic 40.0%
AGGCAAAACCAATTCTCTGT+GGG - chr3.2:33976740-33976759 MS.gene95934:intron 40.0%
CATTTGGAGACGTATTGGAT+TGG - chr3.2:33976135-33976154 MS.gene95934:CDS 40.0%
GAAGAAGCTTATTCATGTCG+CGG - chr3.2:33976205-33976224 MS.gene95934:CDS 40.0%
GATAGGTATGGTGAACGAAT+AGG + chr3.2:33976316-33976335 None:intergenic 40.0%
GTCTTGAGGAAGATACTCAA+GGG - chr3.2:33977160-33977179 MS.gene95934:CDS 40.0%
TAAAGGGAGTGGCAATGATT+AGG + chr3.2:33976712-33976731 None:intergenic 40.0%
TACCACGTTTACGATAGGTA+TGG + chr3.2:33976328-33976347 None:intergenic 40.0%
TCCACAATGTGCCAATATTC+GGG + chr3.2:33976187-33976206 None:intergenic 40.0%
TCTTGAGGAAGATACTCAAG+GGG - chr3.2:33977161-33977180 MS.gene95934:CDS 40.0%
TGCAACATTTGGAGACGTAT+TGG - chr3.2:33976130-33976149 MS.gene95934:CDS 40.0%
TGTCTTGAGGAAGATACTCA+AGG - chr3.2:33977159-33977178 MS.gene95934:CDS 40.0%
TTATCTTCTATTCCGGGAAG+TGG - chr3.2:33976671-33976690 MS.gene95934:intron 40.0%
! CTTCTTGTTAGTGCTTTTGG+TGG - chr3.2:33976782-33976801 MS.gene95934:intron 40.0%
! CTTGGTTGAGTTGTTTCGTT+AGG - chr3.2:33976529-33976548 MS.gene95934:intron 40.0%
! GGATTAGGTTTGTCACTTGT+GGG - chr3.2:33976434-33976453 MS.gene95934:intron 40.0%
! GTCCTCCGAGATTTTTCTTT+TGG - chr3.2:33976855-33976874 MS.gene95934:intron 40.0%
! TGGATTAGGTTTGTCACTTG+TGG - chr3.2:33976433-33976452 MS.gene95934:intron 40.0%
! TGGTTGAGTTGTTTCGTTAG+GGG - chr3.2:33976531-33976550 MS.gene95934:intron 40.0%
! TGTGGTATAGGCCTTTTAAC+TGG - chr3.2:33976470-33976489 MS.gene95934:intron 40.0%
! TTTTGCCTCCCACTCATAAA+GGG + chr3.2:33976728-33976747 None:intergenic 40.0%
!! TTGTTAGTGCTTTTGGTGGT+AGG - chr3.2:33976786-33976805 MS.gene95934:intron 40.0%
!!! GGCCAAGTATTTTGATGATG+AGG - chr3.2:33977126-33977145 MS.gene95934:CDS 40.0%
!!! TTCTCTGTGGGAAGTGTTTT+TGG - chr3.2:33976752-33976771 MS.gene95934:intron 40.0%
AAAATCCGCACCCCAAGATT+AGG + chr3.2:33976892-33976911 None:intergenic 45.0%
AAGGTGAAGAGGAAGAGGAA+GGG - chr3.2:33976267-33976286 MS.gene95934:intron 45.0%
AGCGATACCACGTTTACGAT+AGG + chr3.2:33976333-33976352 None:intergenic 45.0%
ATAGGGAGGCGATACTAAAG+TGG + chr3.2:33976298-33976317 None:intergenic 45.0%
ATTGCCACTCCCTTTATGAG+TGG - chr3.2:33976716-33976735 MS.gene95934:intron 45.0%
CACCATACCTATCGTAAACG+TGG - chr3.2:33976323-33976342 MS.gene95934:intron 45.0%
GAGGCAAAACCAATTCTCTG+TGG - chr3.2:33976739-33976758 MS.gene95934:intron 45.0%
TAGCGTATAGGGTGTGGTAT+AGG - chr3.2:33976458-33976477 MS.gene95934:intron 45.0%
TATTCCGGGAAGTGGTTCTT+CGG - chr3.2:33976679-33976698 MS.gene95934:intron 45.0%
TGTTTCCTAATCTTGGGGTG+CGG - chr3.2:33976884-33976903 MS.gene95934:intron 45.0%
TTACACACTTCACGAGGTGA+AGG - chr3.2:33977105-33977124 MS.gene95934:CDS 45.0%
TTGCCACTCCCTTTATGAGT+GGG - chr3.2:33976717-33976736 MS.gene95934:intron 45.0%
! GTTTTGCCTCCCACTCATAA+AGG + chr3.2:33976729-33976748 None:intergenic 45.0%
! TGAGTTGTTTCGTTAGGGGT+GGG - chr3.2:33976535-33976554 MS.gene95934:intron 45.0%
! TTGAGTTGTTTCGTTAGGGG+TGG - chr3.2:33976534-33976553 MS.gene95934:intron 45.0%
!! CACTTGTGGGTTAGCGTATA+GGG - chr3.2:33976447-33976466 MS.gene95934:intron 45.0%
!! CTTTTCCATGGTGTTTTGGG+CGG + chr3.2:33976372-33976391 None:intergenic 45.0%
!! TCACTTGTGGGTTAGCGTAT+AGG - chr3.2:33976446-33976465 MS.gene95934:intron 45.0%
AAGAGCAACTCTGCAGCTCT+TGG - chr3.2:33977064-33977083 MS.gene95934:CDS 50.0%
AGGAGGACGCTATTGTCTTG+AGG - chr3.2:33977146-33977165 MS.gene95934:CDS 50.0%
CGCCCAAAACACCATGGAAA+AGG - chr3.2:33976370-33976389 MS.gene95934:intron 50.0%
CTCGTTACTCGCCCGAATAT+TGG - chr3.2:33976173-33976192 MS.gene95934:CDS 50.0%
CTTCCTCTTCACCTTCAGAG+AGG + chr3.2:33976262-33976281 None:intergenic 50.0%
GAAGAGGAAGAGGAAGGGAA+TGG - chr3.2:33976272-33976291 MS.gene95934:intron 50.0%
GAAGGTGAAGAGGAAGAGGA+AGG - chr3.2:33976266-33976285 MS.gene95934:intron 50.0%
GCCCGAATATTGGCACATTG+TGG - chr3.2:33976183-33976202 MS.gene95934:CDS 50.0%
GGTATGGTGAACGAATAGGG+AGG + chr3.2:33976312-33976331 None:intergenic 50.0%
GTTGCTTACCTCCTCTCTGA+AGG - chr3.2:33976248-33976267 MS.gene95934:CDS 50.0%
TTCACCGAAGAACCACTTCC+CGG + chr3.2:33976686-33976705 None:intergenic 50.0%
! CTCTGAAGGTGAAGAGGAAG+AGG - chr3.2:33976262-33976281 MS.gene95934:intron 50.0%
! TTGGGGTGCGGATTTTCTCT+AGG - chr3.2:33976896-33976915 MS.gene95934:intron 50.0%
!! GATGGGTTACACACTTCACG+AGG - chr3.2:33977099-33977118 MS.gene95934:CDS 50.0%
!! GTCTTGTCTAGGGAGTGTTC+TGG - chr3.2:33976833-33976852 MS.gene95934:intron 50.0%
AAGACGTCTCAGTGCACGTC+CGG - chr3.2:33977041-33977060 MS.gene95934:CDS 55.0%
AAGGTCTGTACAGCAGCTGC+AGG + chr3.2:33976939-33976958 None:intergenic 55.0%
CATGTCGCGGACAATTGCAG+CGG - chr3.2:33976218-33976237 MS.gene95934:CDS 55.0%
CCACTCCCTTTATGAGTGGG+AGG - chr3.2:33976720-33976739 MS.gene95934:intron 55.0%
CCTCCCACTCATAAAGGGAG+TGG + chr3.2:33976723-33976742 None:intergenic 55.0%
CCTCTTCACCTTCAGAGAGG+AGG + chr3.2:33976259-33976278 None:intergenic 55.0%
CTGCAGGTCTCAAGGTAGAG+AGG + chr3.2:33976923-33976942 None:intergenic 55.0%
GAGCTGCAGAGTTGCTCTTC+CGG + chr3.2:33977063-33977082 None:intergenic 55.0%
GTGGGAAAGCAGAGTAGGCT+AGG - chr3.2:33976553-33976572 MS.gene95934:intron 55.0%
TACAGCAGCTGCAGGTCTCA+AGG + chr3.2:33976931-33976950 None:intergenic 55.0%
TAGGGGTGGGAAAGCAGAGT+AGG - chr3.2:33976548-33976567 MS.gene95934:intron 55.0%
! CCTCCTCTCTGAAGGTGAAG+AGG - chr3.2:33976256-33976275 MS.gene95934:intron 55.0%
!! GTGGGTTAGCGTATAGGGTG+TGG - chr3.2:33976452-33976471 MS.gene95934:intron 55.0%
!! TTCCATGGTGTTTTGGGCGG+CGG + chr3.2:33976369-33976388 None:intergenic 55.0%
CACCGCCGCCCAAAACACCA+TGG - chr3.2:33976364-33976383 MS.gene95934:intron 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 33976101 33977201 33976101 ID=MS.gene95934
chr3.2 mRNA 33976101 33977201 33976101 ID=MS.gene95934.t1;Parent=MS.gene95934
chr3.2 exon 33976911 33977201 33976911 ID=MS.gene95934.t1.exon1;Parent=MS.gene95934.t1
chr3.2 CDS 33976911 33977201 33976911 ID=cds.MS.gene95934.t1;Parent=MS.gene95934.t1
chr3.2 exon 33976101 33976271 33976101 ID=MS.gene95934.t1.exon2;Parent=MS.gene95934.t1
chr3.2 CDS 33976101 33976271 33976101 ID=cds.MS.gene95934.t1;Parent=MS.gene95934.t1
Gene Sequence

>MS.gene95934

ATGTCTTCAAAGAAGGTGTGCATAAGAGTTGCAACATTTGGAGACGTATTGGATTGGAACAGTAACAACAATCTCGTTACTCGCCCGAATATTGGCACATTGTGGAAGAAGCTTATTCATGTCGCGGACAATTGCAGCGGCTGTATTGTTGCTTACCTCCTCTCTGAAGGTGAAGAGGAAGAGGAAGGGAATGGCCACTTTAGTATCGCCTCCCTATTCGTTCACCATACCTATCGTAAACGTGGTATCGCTACTGATCTTATCACCGCCGCCCAAAACACCATGGAAAAGGTTAGGTTTGTTTCTTTATATTCTGTTATAATTTTGTTTTTTGGATTAGGTTTGTCACTTGTGGGTTAGCGTATAGGGTGTGGTATAGGCCTTTTAACTGGTTATGTATGGAGTTTGTTTAATGTTTAGGTATGAAGCTTGGTTGAGTTGTTTCGTTAGGGGTGGGAAAGCAGAGTAGGCTAGGTTTTTTGTTAATCTAGCACGCCACTGTGTGATCTATCTGAAAACCCGTGATGATTTTCTGTTTTCTGGTAGAATCTGAGTTTCACTATGCTAGGATTATCTTCTATTCCGGGAAGTGGTTCTTCGGTGAATTCCCTAATCATTGCCACTCCCTTTATGAGTGGGAGGCAAAACCAATTCTCTGTGGGAAGTGTTTTTGGCTTCTTTCTTCTTGTTAGTGCTTTTGGTGGTAGGTGTTTGTTGTTTTTTCTGTAGTTAGTCTTGTCTAGGGAGTGTTCTGGTCCTCCGAGATTTTTCTTTTGGTTTTCTTGTTTCCTAATCTTGGGGTGCGGATTTTCTCTAGGTCCTCTCTACCTTGAGACCTGCAGCTGCTGTACAGACCTTTTTTGATGTTTTTGGTTGTCAATACATATTTTTTCCATTAAAAGTCTGTTACAATGTGTAATTTGACTGAAGGTGGGTGCTAAAGACGTCTCAGTGCACGTCCGGAAGAGCAACTCTGCAGCTCTTGGTTTGTTTGAAAAGATGGGTTACACACTTCACGAGGTGAAGGCCAAGTATTTTGATGATGAGGAGGACGCTATTGTCTTGAGGAAGATACTCAAGGGGAAGCATGTTGCCACCTAA

Protein sequence

>MS.gene95934.t1

MSSKKVCIRVATFGDVLDWNSNNNLVTRPNIGTLWKKLIHVADNCSGCIVAYLLSEGEEEEEGNGHFSIASLFVHHTYRKRGIATDLITAAQNTMEKVGAKDVSVHVRKSNSAALGLFEKMGYTLHEVKAKYFDDEEDAIVLRKILKGKHVAT