AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99462


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99462.t1 MTR_4g019080 95.415 349 15 1 3 350 2 350 0.0 675
MS.gene99462.t1 MTR_7g066210 47.967 369 149 11 7 350 2 352 4.26e-105 312
MS.gene99462.t1 MTR_7g011345 85.000 80 12 0 252 331 1 80 3.38e-41 139
MS.gene99462.t1 MTR_3g069910 30.395 329 178 13 43 341 29 336 1.71e-26 108
MS.gene99462.t1 MTR_5g068770 26.877 253 135 11 32 243 33 276 1.74e-13 72.0
MS.gene99462.t1 MTR_5g077100 29.630 216 116 9 51 242 45 248 1.21e-12 69.3
MS.gene99462.t1 MTR_7g062940 23.985 271 150 8 11 242 2 255 3.34e-11 65.1
MS.gene99462.t1 MTR_8g067720 25.385 390 208 18 1 348 1 349 5.76e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99462.t1 AT5G01090 35.674 356 200 11 8 344 6 351 3.25e-59 195
MS.gene99462.t1 AT3G09190 35.159 347 199 11 11 337 4 344 2.48e-52 177
MS.gene99462.t1 AT3G09035 38.123 341 177 12 11 327 4 334 1.15e-43 154
MS.gene99462.t1 AT3G54080 26.959 319 189 10 39 337 40 334 1.07e-22 97.8
MS.gene99462.t1 AT5G03140 33.663 202 100 10 51 228 51 242 3.91e-15 77.0
MS.gene99462.t1 AT2G29250 30.488 246 134 10 32 242 18 261 1.36e-12 69.3
MS.gene99462.t1 AT5G60270 27.843 255 143 11 51 272 41 287 3.77e-12 67.8
MS.gene99462.t1 AT2G37710 27.511 229 132 8 66 265 62 285 8.36e-12 67.0
MS.gene99462.t1 AT4G02420 27.542 236 120 10 45 242 38 260 1.62e-11 65.9
MS.gene99462.t1 AT5G55830 28.994 338 176 19 8 294 2 326 2.42e-11 65.5
MS.gene99462.t1 AT2G29220 30.000 270 143 13 9 242 2 261 3.89e-11 64.7
MS.gene99462.t1 AT3G53810 28.837 215 114 9 66 249 62 268 5.14e-11 64.3

Find 54 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTTGCTGCAATGATATT+TGG 0.190612 4.2:-83361782 MS.gene99462:CDS
GAACAAAATGATGAAAATTA+AGG 0.213685 4.2:+83362603 None:intergenic
AAGATTCAATTCTCTGAATC+TGG 0.271114 4.2:-83362460 MS.gene99462:CDS
CTTTATTTGTGGACAATCTT+TGG 0.296212 4.2:-83361725 MS.gene99462:CDS
AATGGTGTCAAAAGTGTTAA+AGG 0.299364 4.2:-83362307 MS.gene99462:CDS
GCAAAGGAAAACAAAGGAAT+TGG 0.308883 4.2:+83362559 None:intergenic
AAGGAATTGGGTCAAAAGAT+AGG 0.346432 4.2:+83362572 None:intergenic
GTGGTGAAAAGTTGCATACT+TGG 0.363123 4.2:-83362120 MS.gene99462:CDS
CATTCACTGTGCTTTATTTG+TGG 0.378610 4.2:-83361736 MS.gene99462:CDS
GGTTGCATAGAACATCCTTC+TGG 0.388793 4.2:+83361680 None:intergenic
GAAAATGAAACTAATTGCTT+AGG 0.395154 4.2:+83362394 None:intergenic
AATTAAGGATTTGAAGTAAA+GGG 0.401934 4.2:+83362618 None:intergenic
TGACATATCTAATGTCTTGA+AGG 0.404093 4.2:-83362005 MS.gene99462:CDS
AAGGATGAAGAAATGTTTGT+TGG 0.406456 4.2:-83361986 MS.gene99462:CDS
TTTACCTTGAATAACCTTGA+TGG 0.409606 4.2:+83362417 None:intergenic
AAAGCTACTTTACCAATTTG+AGG 0.410593 4.2:-83362150 MS.gene99462:CDS
CTGAAATTTAAGTCCAATGT+TGG 0.412543 4.2:-83362520 MS.gene99462:CDS
TTCAATCTCAATGGAAAATG+AGG 0.414272 4.2:-83362356 MS.gene99462:CDS
AGATTCAGAGAATTGAATCT+TGG 0.417959 4.2:+83362462 None:intergenic
ATTCATGGAGCTTTGTTATG+AGG 0.423293 4.2:-83361928 MS.gene99462:CDS
TTCCTATACTCAACATCTAA+AGG 0.425116 4.2:+83361659 None:intergenic
AGCCATTGGATCCATTGTTA+TGG 0.433636 4.2:-83362036 MS.gene99462:CDS
GCTGCAATGATATTTGGTAC+TGG 0.447642 4.2:-83361776 MS.gene99462:CDS
ATGATATTTGGTACTGGTTG+TGG 0.463600 4.2:-83361770 MS.gene99462:CDS
GTTGTAGATAATAAAACCAT+TGG 0.483802 4.2:-83361626 MS.gene99462:CDS
ACATGGAAATTCAAAGCCAT+TGG 0.487541 4.2:-83362050 MS.gene99462:CDS
CAAAGGAAAACAAAGGAATT+GGG 0.488244 4.2:+83362560 None:intergenic
TTGATTATGAAGCAAGTTCA+AGG 0.491101 4.2:-83362096 MS.gene99462:CDS
TTACCTTGAATAACCTTGAT+GGG 0.491388 4.2:+83362418 None:intergenic
AACCTTTAGATGTTGAGTAT+AGG 0.496316 4.2:-83361661 MS.gene99462:CDS
GGTTGTTAAACCAAAGGAAA+AGG 0.497433 4.2:-83361831 MS.gene99462:CDS
AAACCCATCAAGGTTATTCA+AGG 0.503209 4.2:-83362421 MS.gene99462:CDS
AGGAATTGGGTCAAAAGATA+GGG 0.505116 4.2:+83362573 None:intergenic
TGCCATAACAATGGATCCAA+TGG 0.520573 4.2:+83362034 None:intergenic
AGATTCAATTCTCTGAATCT+GGG 0.525904 4.2:-83362459 MS.gene99462:CDS
GATAATAAAACCATTGGAGA+TGG 0.530127 4.2:-83361620 MS.gene99462:CDS
TTCAATGGTTGTTAAACCAA+AGG 0.530255 4.2:-83361837 MS.gene99462:CDS
CTACTTATTACCATCTCCAA+TGG 0.535555 4.2:+83361610 None:intergenic
AAATTAAGGATTTGAAGTAA+AGG 0.544616 4.2:+83362617 None:intergenic
GTTGGATTTAGTCATGTGAA+GGG 0.567364 4.2:-83361968 MS.gene99462:CDS
TGTTGGATTTAGTCATGTGA+AGG 0.574358 4.2:-83361969 MS.gene99462:CDS
ACAGAAACATTAGGATCAAG+TGG 0.578057 4.2:+83361875 None:intergenic
TCAATCTCAATGGAAAATGA+GGG 0.579881 4.2:-83362355 MS.gene99462:CDS
TTGGATTTAGTCATGTGAAG+GGG 0.605657 4.2:-83361967 MS.gene99462:CDS
GAAGCGAGACTGAGTAAACA+TGG 0.608963 4.2:-83362067 MS.gene99462:CDS
GATTTACAAGAAACCCATCA+AGG 0.610971 4.2:-83362431 MS.gene99462:CDS
ATCTCAATGGAAAATGAGGG+TGG 0.611815 4.2:-83362352 MS.gene99462:CDS
CGTGGTGCAGTTGTTCCAGA+AGG 0.612769 4.2:-83361695 MS.gene99462:CDS
ATTACCATAAAATCCAACAT+TGG 0.612888 4.2:+83362507 None:intergenic
AAATCAGCAAAGGAAAACAA+AGG 0.645808 4.2:+83362553 None:intergenic
ACAATCTTTGGCAATAAACG+TGG 0.699558 4.2:-83361713 MS.gene99462:CDS
GGCTCTGAATGCATAGAATG+TGG 0.706249 4.2:+83361896 None:intergenic
ATTATGAAGCAAGTTCAAGG+AGG 0.716891 4.2:-83362093 MS.gene99462:CDS
TCTGAATGCATAGAATGTGG+TGG 0.723371 4.2:+83361899 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAAAAATAATGATTTTCA+AGG - chr4.2:83362006-83362025 MS.gene99462:CDS 15.0%
!! GAACAAAATGATGAAAATTA+AGG + chr4.2:83361650-83361669 None:intergenic 20.0%
!! TAAAAAAAGTGAAACTTCAA+TGG - chr4.2:83362398-83362417 MS.gene99462:CDS 20.0%
!!! AAATTAAGGATTTGAAGTAA+AGG + chr4.2:83361636-83361655 None:intergenic 20.0%
!!! AATGAGATTTTGATTAGAAA+TGG - chr4.2:83362066-83362085 MS.gene99462:CDS 20.0%
!!! AATTAAGGATTTGAAGTAAA+GGG + chr4.2:83361635-83361654 None:intergenic 20.0%
!!! CTTTTTTTAACAGAAACATT+AGG + chr4.2:83362387-83362406 None:intergenic 20.0%
!!! TTTTACATTTTCAATCTCAA+TGG - chr4.2:83361885-83361904 MS.gene99462:CDS 20.0%
!!! TTTTTAATCAAAGTTCATCT+GGG - chr4.2:83361971-83361990 MS.gene99462:CDS 20.0%
! ATTACCATAAAATCCAACAT+TGG + chr4.2:83361746-83361765 None:intergenic 25.0%
! GAAAATGAAACTAATTGCTT+AGG + chr4.2:83361859-83361878 None:intergenic 25.0%
! GTTGTAGATAATAAAACCAT+TGG - chr4.2:83362624-83362643 MS.gene99462:CDS 25.0%
!! ATAATGCTTTTCTGTATTCA+TGG - chr4.2:83362307-83362326 MS.gene99462:CDS 25.0%
!! GTTTTTAATCAAAGTTCATC+TGG - chr4.2:83361970-83361989 MS.gene99462:CDS 25.0%
!!! ATCTTTTTCAAAATCAGCAA+AGG + chr4.2:83361710-83361729 None:intergenic 25.0%
AAATCAGCAAAGGAAAACAA+AGG + chr4.2:83361700-83361719 None:intergenic 30.0%
AACCTTTAGATGTTGAGTAT+AGG - chr4.2:83362589-83362608 MS.gene99462:CDS 30.0%
AAGATTCAATTCTCTGAATC+TGG - chr4.2:83361790-83361809 MS.gene99462:CDS 30.0%
AAGGATGAAGAAATGTTTGT+TGG - chr4.2:83362264-83362283 MS.gene99462:CDS 30.0%
AGATTCAATTCTCTGAATCT+GGG - chr4.2:83361791-83361810 MS.gene99462:CDS 30.0%
AGATTCAGAGAATTGAATCT+TGG + chr4.2:83361791-83361810 None:intergenic 30.0%
CAAAGGAAAACAAAGGAATT+GGG + chr4.2:83361693-83361712 None:intergenic 30.0%
CTGAAATTTAAGTCCAATGT+TGG - chr4.2:83361730-83361749 MS.gene99462:CDS 30.0%
GATAATAAAACCATTGGAGA+TGG - chr4.2:83362630-83362649 MS.gene99462:CDS 30.0%
GGTAATGCAAAAATTGTCAA+TGG - chr4.2:83361760-83361779 MS.gene99462:CDS 30.0%
TCAATCTCAATGGAAAATGA+GGG - chr4.2:83361895-83361914 MS.gene99462:CDS 30.0%
TGACATATCTAATGTCTTGA+AGG - chr4.2:83362245-83362264 MS.gene99462:CDS 30.0%
TTACCTTGAATAACCTTGAT+GGG + chr4.2:83361835-83361854 None:intergenic 30.0%
TTCAATCTCAATGGAAAATG+AGG - chr4.2:83361894-83361913 MS.gene99462:CDS 30.0%
TTCAATGGTTGTTAAACCAA+AGG - chr4.2:83362413-83362432 MS.gene99462:CDS 30.0%
TTCCTATACTCAACATCTAA+AGG + chr4.2:83362594-83362613 None:intergenic 30.0%
TTGATTATGAAGCAAGTTCA+AGG - chr4.2:83362154-83362173 MS.gene99462:CDS 30.0%
TTTACCTTGAATAACCTTGA+TGG + chr4.2:83361836-83361855 None:intergenic 30.0%
! AAAGCTACTTTACCAATTTG+AGG - chr4.2:83362100-83362119 MS.gene99462:CDS 30.0%
! AAGTCCAATGTTGGATTTTA+TGG - chr4.2:83361739-83361758 MS.gene99462:CDS 30.0%
! CTTTATTTGTGGACAATCTT+TGG - chr4.2:83362525-83362544 MS.gene99462:CDS 30.0%
! TCAATTTTGTGCCATAACAA+TGG + chr4.2:83362228-83362247 None:intergenic 30.0%
! TTAACACTTTTGACACCATT+TGG + chr4.2:83361943-83361962 None:intergenic 30.0%
!! AATGGTGTCAAAAGTGTTAA+AGG - chr4.2:83361943-83361962 MS.gene99462:CDS 30.0%
!! GCTTTTGTTATGATTCCAAA+TGG - chr4.2:83361925-83361944 MS.gene99462:CDS 30.0%
!! TGTTGAGTTTTGTGGTTATA+AGG - chr4.2:83362035-83362054 MS.gene99462:CDS 30.0%
AAACCCATCAAGGTTATTCA+AGG - chr4.2:83361829-83361848 MS.gene99462:CDS 35.0%
AAGGAATTGGGTCAAAAGAT+AGG + chr4.2:83361681-83361700 None:intergenic 35.0%
ACAATCTTTGGCAATAAACG+TGG - chr4.2:83362537-83362556 MS.gene99462:CDS 35.0%
ACAGAAACATTAGGATCAAG+TGG + chr4.2:83362378-83362397 None:intergenic 35.0%
ACATGGAAATTCAAAGCCAT+TGG - chr4.2:83362200-83362219 MS.gene99462:CDS 35.0%
AGGAATTGGGTCAAAAGATA+GGG + chr4.2:83361680-83361699 None:intergenic 35.0%
ATTATGAAGCAAGTTCAAGG+AGG - chr4.2:83362157-83362176 MS.gene99462:CDS 35.0%
CTACTTATTACCATCTCCAA+TGG + chr4.2:83362643-83362662 None:intergenic 35.0%
GATTTACAAGAAACCCATCA+AGG - chr4.2:83361819-83361838 MS.gene99462:CDS 35.0%
GCAAAGGAAAACAAAGGAAT+TGG + chr4.2:83361694-83361713 None:intergenic 35.0%
GGTTGTTAAACCAAAGGAAA+AGG - chr4.2:83362419-83362438 MS.gene99462:CDS 35.0%
GTTCTTGCTGCAATGATATT+TGG - chr4.2:83362468-83362487 MS.gene99462:CDS 35.0%
GTTGGATTTAGTCATGTGAA+GGG - chr4.2:83362282-83362301 MS.gene99462:CDS 35.0%
TGTTGGATTTAGTCATGTGA+AGG - chr4.2:83362281-83362300 MS.gene99462:CDS 35.0%
TTGGATTTAGTCATGTGAAG+GGG - chr4.2:83362283-83362302 MS.gene99462:CDS 35.0%
! ACCAATTTGAGGCTAAAAAG+TGG - chr4.2:83362111-83362130 MS.gene99462:CDS 35.0%
! TTCTTCTTTGCCTTTTCCTT+TGG + chr4.2:83362432-83362451 None:intergenic 35.0%
!! ATGATATTTGGTACTGGTTG+TGG - chr4.2:83362480-83362499 MS.gene99462:CDS 35.0%
!! ATTCATGGAGCTTTGTTATG+AGG - chr4.2:83362322-83362341 MS.gene99462:CDS 35.0%
!! CATTCACTGTGCTTTATTTG+TGG - chr4.2:83362514-83362533 MS.gene99462:CDS 35.0%
!! GAGTTTTGTGGTTATAAGGA+TGG - chr4.2:83362039-83362058 MS.gene99462:CDS 35.0%
!! GGTATTAGTGTTGAGTTTTG+TGG - chr4.2:83362027-83362046 MS.gene99462:CDS 35.0%
!!! ACCACTTTTTAGCCTCAAAT+TGG + chr4.2:83362115-83362134 None:intergenic 35.0%
AGCCATTGGATCCATTGTTA+TGG - chr4.2:83362214-83362233 MS.gene99462:CDS 40.0%
ATCTCAATGGAAAATGAGGG+TGG - chr4.2:83361898-83361917 MS.gene99462:CDS 40.0%
GCTGCAATGATATTTGGTAC+TGG - chr4.2:83362474-83362493 MS.gene99462:CDS 40.0%
GTGGTGAAAAGTTGCATACT+TGG - chr4.2:83362130-83362149 MS.gene99462:CDS 40.0%
TCTGAATGCATAGAATGTGG+TGG + chr4.2:83362354-83362373 None:intergenic 40.0%
TGCCATAACAATGGATCCAA+TGG + chr4.2:83362219-83362238 None:intergenic 40.0%
! AGTTCATCTGGGTTTTCTCT+TGG - chr4.2:83361982-83362001 MS.gene99462:CDS 40.0%
GAAGCGAGACTGAGTAAACA+TGG - chr4.2:83362183-83362202 MS.gene99462:CDS 45.0%
GGTTGCATAGAACATCCTTC+TGG + chr4.2:83362573-83362592 None:intergenic 45.0%
!! GGCTCTGAATGCATAGAATG+TGG + chr4.2:83362357-83362376 None:intergenic 45.0%
CGTGGTGCAGTTGTTCCAGA+AGG - chr4.2:83362555-83362574 MS.gene99462:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 83361610 83362662 83361610 ID=MS.gene99462
chr4.2 mRNA 83361610 83362662 83361610 ID=MS.gene99462.t1;Parent=MS.gene99462
chr4.2 exon 83361610 83362662 83361610 ID=MS.gene99462.t1.exon1;Parent=MS.gene99462.t1
chr4.2 CDS 83361610 83362662 83361610 ID=cds.MS.gene99462.t1;Parent=MS.gene99462.t1
Gene Sequence

>MS.gene99462

ATGGCTTCTTCATTCACAACTACCCTTTACTTCAAATCCTTAATTTTCATCATTTTGTTCATAAAAACCCTATCTTTTGACCCAATTCCTTTGTTTTCCTTTGCTGATTTTGAAAAAGATCTGAAATTTAAGTCCAATGTTGGATTTTATGGTAATGCAAAAATTGTCAATGGTGAGTCCAAGATTCAATTCTCTGAATCTGGGAGACTGATTTACAAGAAACCCATCAAGGTTATTCAAGGTAAACCTAAGCAATTAGTTTCATTTTCAACTTATTTTACATTTTCAATCTCAATGGAAAATGAGGGTGGTTTAGCTTTTGTTATGATTCCAAATGGTGTCAAAAGTGTTAAAGGTGATGTTTTTAATCAAAGTTCATCTGGGTTTTCTCTTGGATTGAAAAATAATGATTTTCAAGGTATTAGTGTTGAGTTTTGTGGTTATAAGGATGGTAAAAATGAGATTTTGATTAGAAATGGTGTTTCAAGTGAAAGCTACTTTACCAATTTGAGGCTAAAAAGTGGTGAAAAGTTGCATACTTGGATTGATTATGAAGCAAGTTCAAGGAGGTTAGAAGCGAGACTGAGTAAACATGGAAATTCAAAGCCATTGGATCCATTGTTATGGCACAAAATTGACATATCTAATGTCTTGAAGGATGAAGAAATGTTTGTTGGATTTAGTCATGTGAAGGGGAATAATGCTTTTCTGTATTCATGGAGCTTTGTTATGAGGAATTTTCCACCACATTCTATGCATTCAGAGCCACTTGATCCTAATGTTTCTGTTAAAAAAAGTGAAACTTCAATGGTTGTTAAACCAAAGGAAAAGGCAAAGAAGAATGATTGTGTTTTAAGAGTTCTTGCTGCAATGATATTTGGTACTGGTTGTGGAGCATTGACAGCATTCACTGTGCTTTATTTGTGGACAATCTTTGGCAATAAACGTGGTGCAGTTGTTCCAGAAGGATGTTCTATGCAACCTTTAGATGTTGAGTATAGGAAAGTTAAAATTGTTGTAGATAATAAAACCATTGGAGATGGTAATAAGTAG

Protein sequence

>MS.gene99462.t1

MASSFTTTLYFKSLIFIILFIKTLSFDPIPLFSFADFEKDLKFKSNVGFYGNAKIVNGESKIQFSESGRLIYKKPIKVIQGKPKQLVSFSTYFTFSISMENEGGLAFVMIPNGVKSVKGDVFNQSSSGFSLGLKNNDFQGISVEFCGYKDGKNEILIRNGVSSESYFTNLRLKSGEKLHTWIDYEASSRRLEARLSKHGNSKPLDPLLWHKIDISNVLKDEEMFVGFSHVKGNNAFLYSWSFVMRNFPPHSMHSEPLDPNVSVKKSETSMVVKPKEKAKKNDCVLRVLAAMIFGTGCGALTAFTVLYLWTIFGNKRGAVVPEGCSMQPLDVEYRKVKIVVDNKTIGDGNK