Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004495.01.T01 | KEH43028.1 | 97.345 | 113 | 3 | 0 | 1 | 113 | 1 | 113 | 2.48E-75 | 230 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004495.01.T01 | A0A072VNN2 | 97.345 | 113 | 3 | 0 | 1 | 113 | 1 | 113 | 1.18e-75 | 230 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000479.01 | MsG0180004495.01 | 0.815779 | 7.762460e-52 | 5.402432e-49 |
| MsG0180002989.01 | MsG0180004495.01 | 0.800659 | 1.299507e-48 | 6.084911e-46 |
| MsG0180004147.01 | MsG0180004495.01 | 0.853210 | 2.778314e-61 | 5.957714e-58 |
| MsG0180004181.01 | MsG0180004495.01 | 0.800330 | 1.516233e-48 | 7.040884e-46 |
| MsG0180004258.01 | MsG0180004495.01 | 0.846918 | 1.606209e-59 | 2.807590e-56 |
| MsG0180004388.01 | MsG0180004495.01 | 0.800299 | 1.537939e-48 | 7.136264e-46 |
| MsG0180004495.01 | MsG0180004949.01 | 0.805178 | 1.513254e-49 | 7.952621e-47 |
| MsG0180004495.01 | MsG0180005692.01 | 0.801043 | 1.084392e-48 | 5.127704e-46 |
| MsG0180004495.01 | MsG0180006226.01 | -0.810363 | 1.196449e-50 | 7.197691e-48 |
| MsG0180004495.01 | MsG0280006436.01 | 0.810855 | 9.366791e-51 | 5.709676e-48 |
| MsG0180004495.01 | MsG0280006586.01 | 0.853115 | 2.956901e-61 | 6.319422e-58 |
| MsG0180004495.01 | MsG0280007481.01 | 0.804750 | 1.859308e-49 | 9.664822e-47 |
| MsG0180004495.01 | MsG0280007609.01 | 0.820216 | 7.713540e-53 | 6.065777e-50 |
| MsG0180004495.01 | MsG0280008270.01 | 0.809771 | 1.604754e-50 | 9.506195e-48 |
| MsG0180004495.01 | MsG0280008590.01 | 0.812220 | 4.730761e-51 | 2.990434e-48 |
| MsG0180004495.01 | MsG0280009509.01 | 0.805196 | 1.500486e-49 | 7.889069e-47 |
| MsG0180004495.01 | MsG0280009514.01 | 0.824167 | 9.347751e-54 | 8.216101e-51 |
| MsG0180004495.01 | MsG0280010391.01 | 0.821401 | 4.117513e-53 | 3.346347e-50 |
| MsG0180004495.01 | MsG0280010459.01 | 0.809644 | 1.708773e-50 | 1.008845e-47 |
| MsG0180004495.01 | MsG0280010614.01 | 0.813810 | 2.120073e-51 | 1.398577e-48 |
| MsG0180004495.01 | MsG0280011348.01 | 0.814749 | 1.314958e-51 | 8.897297e-49 |
| MsG0180004495.01 | MsG0380012021.01 | 0.816610 | 5.060121e-52 | 3.602425e-49 |
| MsG0180004495.01 | MsG0380012033.01 | 0.824861 | 6.415366e-54 | 5.752596e-51 |
| MsG0180004495.01 | MsG0380014163.01 | 0.819578 | 1.078915e-52 | 8.335392e-50 |
| MsG0180004495.01 | MsG0380014754.01 | 0.803431 | 3.498309e-49 | 1.757636e-46 |
| MsG0180004495.01 | MsG0380015931.01 | 0.802337 | 5.884030e-49 | 2.875031e-46 |
| MsG0180004495.01 | MsG0480018230.01 | 0.802965 | 4.368067e-49 | 2.168567e-46 |
| MsG0180004495.01 | MsG0480018780.01 | 0.800699 | 1.274744e-48 | 5.974836e-46 |
| MsG0180004495.01 | MsG0480019034.01 | 0.840676 | 7.531292e-58 | 1.080728e-54 |
| MsG0180004495.01 | MsG0480020445.01 | 0.815500 | 8.957423e-52 | 6.186482e-49 |
| MsG0180004495.01 | MsG0480021225.01 | 0.844469 | 7.414821e-59 | 1.198230e-55 |
| MsG0180004495.01 | MsG0480021958.01 | 0.806582 | 7.670788e-50 | 4.178760e-47 |
| MsG0180004495.01 | MsG0480022479.01 | 0.843768 | 1.143596e-58 | 1.807788e-55 |
| MsG0180004495.01 | MsG0480022480.01 | 0.836206 | 1.071733e-56 | 1.341492e-53 |
| MsG0180004495.01 | MsG0480022486.01 | 0.841131 | 5.723285e-58 | 8.328955e-55 |
| MsG0180004495.01 | MsG0480022520.01 | 0.811706 | 6.121976e-51 | 3.816341e-48 |
| MsG0180004495.01 | MsG0480022608.01 | 0.829133 | 6.104484e-55 | 6.192037e-52 |
| MsG0180004495.01 | MsG0480022663.01 | 0.807566 | 4.747196e-50 | 2.652856e-47 |
| MsG0180004495.01 | MsG0480022987.01 | 0.815261 | 1.011958e-51 | 6.942965e-49 |
| MsG0180004495.01 | MsG0480023903.01 | 0.881206 | 2.910522e-70 | 1.689842e-66 |
| MsG0180004495.01 | MsG0580024383.01 | 0.824020 | 1.012185e-53 | 8.858940e-51 |
| MsG0180004495.01 | MsG0580025072.01 | 0.807610 | 4.647488e-50 | 2.600068e-47 |
| MsG0180004495.01 | MsG0580025352.01 | 0.801055 | 1.078650e-48 | 5.101982e-46 |
| MsG0180004495.01 | MsG0580027129.01 | 0.823751 | 1.170389e-53 | 1.016833e-50 |
| MsG0180004495.01 | MsG0580029148.01 | 0.814254 | 1.692263e-51 | 1.129829e-48 |
| MsG0180004495.01 | MsG0580029672.01 | 0.818763 | 1.654324e-52 | 1.249510e-49 |
| MsG0180004495.01 | MsG0580029767.01 | 0.837067 | 6.469464e-57 | 8.308309e-54 |
| MsG0180004495.01 | MsG0580029960.01 | 0.804389 | 2.211058e-49 | 1.138589e-46 |
| MsG0180004495.01 | MsG0680031384.01 | 0.808067 | 3.715785e-50 | 2.103925e-47 |
| MsG0180004495.01 | MsG0780036235.01 | 0.812727 | 3.665486e-51 | 2.348411e-48 |
| MsG0180004495.01 | MsG0780037094.01 | 0.841846 | 3.709535e-58 | 5.520493e-55 |
| MsG0180004495.01 | MsG0780038444.01 | 0.837447 | 5.171584e-57 | 6.719799e-54 |
| MsG0180004495.01 | MsG0780038495.01 | 0.800799 | 1.216493e-48 | 5.716309e-46 |
| MsG0180004495.01 | MsG0780039430.01 | 0.809996 | 1.435300e-50 | 8.552757e-48 |
| MsG0180004495.01 | MsG0780039622.01 | -0.810306 | 1.230875e-50 | 7.393458e-48 |
| MsG0180004495.01 | MsG0780040060.01 | 0.816057 | 6.729454e-52 | 4.719122e-49 |
| MsG0180004495.01 | MsG0780040541.01 | 0.811273 | 7.602338e-51 | 4.685312e-48 |
| MsG0180004495.01 | MsG0780040601.01 | 0.817955 | 2.520829e-52 | 1.861974e-49 |
| MsG0180004495.01 | MsG0880042967.01 | 0.800810 | 1.210341e-48 | 5.688905e-46 |
| MsG0180004495.01 | MsG0880043262.01 | 0.816458 | 5.473575e-52 | 3.880348e-49 |
| MsG0180004495.01 | MsG0880043547.01 | 0.812885 | 3.384096e-51 | 2.177163e-48 |
| MsG0180004495.01 | MsG0880046467.01 | 0.810406 | 1.171095e-50 | 7.052908e-48 |
| MsG0180004495.01 | MsG0880046586.01 | 0.806478 | 8.068581e-50 | 4.383867e-47 |
| MsG0180004495.01 | MsG0880046642.01 | 0.802711 | 4.927650e-49 | 2.430711e-46 |
| MsG0180004495.01 | MsG0880046762.01 | 0.800683 | 1.284699e-48 | 6.019094e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004495.01.T01 | MTR_1g080990 | 97.345 | 113 | 3 | 0 | 1 | 113 | 1 | 113 | 3.00e-79 | 230 |
| MsG0180004495.01.T01 | MTR_1g080990 | 97.321 | 112 | 3 | 0 | 1 | 112 | 1 | 112 | 3.89e-77 | 226 |
| MsG0180004495.01.T01 | MTR_1g080990 | 97.321 | 112 | 3 | 0 | 1 | 112 | 1 | 112 | 4.40e-77 | 224 |
| MsG0180004495.01.T01 | MTR_1g041410 | 46.667 | 105 | 51 | 2 | 12 | 112 | 10 | 113 | 1.17e-25 | 95.9 |
| MsG0180004495.01.T01 | MTR_7g114890 | 45.000 | 120 | 56 | 5 | 1 | 112 | 1 | 118 | 5.80e-25 | 93.2 |
| MsG0180004495.01.T01 | MTR_7g114890 | 45.000 | 120 | 56 | 5 | 1 | 112 | 1 | 118 | 9.15e-25 | 93.6 |
| MsG0180004495.01.T01 | MTR_7g114890 | 45.000 | 120 | 56 | 5 | 1 | 112 | 1 | 118 | 9.20e-25 | 93.6 |
| MsG0180004495.01.T01 | MTR_7g114880 | 40.909 | 88 | 47 | 3 | 1 | 85 | 1 | 86 | 1.42e-14 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004495.01.T01 | AT4G14746 | 34.234 | 111 | 61 | 3 | 10 | 114 | 10 | 114 | 1.29e-14 | 66.6 |
| MsG0180004495.01.T01 | AT4G14746 | 33.333 | 111 | 63 | 3 | 10 | 115 | 10 | 114 | 2.54e-13 | 63.9 |
Find 32 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTTTGTGAATGCGATTC+TGG | 0.220907 | 1:-78869475 | MsG0180004495.01.T01:CDS |
| ATGGGTTCTTTCAAGTTGTT+GGG | 0.370656 | 1:-78869831 | MsG0180004495.01.T01:CDS |
| CTTGTAGGTAGCTTGAATTA+TGG | 0.388414 | 1:-78869235 | MsG0180004495.01.T01:intron |
| AATGGGTTCTTTCAAGTTGT+TGG | 0.394087 | 1:-78869832 | MsG0180004495.01.T01:CDS |
| ATATATCATCATCTTCATCT+TGG | 0.410673 | 1:+78869440 | None:intergenic |
| TATATCATCATCTTCATCTT+GGG | 0.418577 | 1:+78869441 | None:intergenic |
| TTGCGAGGTAAAGTCTTCTC+TGG | 0.418920 | 1:+78869187 | None:intergenic |
| TCTGGAACTGGAGGAGGTGC+TGG | 0.422912 | 1:+78869205 | None:intergenic |
| AAAATCCCCTTTGGCAGCCA+TGG | 0.429161 | 1:+78869776 | None:intergenic |
| GGTAAAGTCTTCTCTGGAAC+TGG | 0.430461 | 1:+78869193 | None:intergenic |
| TACTTCCCATGGCTGCCAAA+GGG | 0.472045 | 1:-78869782 | MsG0180004495.01.T01:CDS |
| GTCTTCTCTGGAACTGGAGG+AGG | 0.479737 | 1:+78869199 | None:intergenic |
| ATCATCAACAAAATCCCCTT+TGG | 0.497557 | 1:+78869767 | None:intergenic |
| GTTGTTGGGTCTTATAGCCA+TGG | 0.505786 | 1:-78869817 | MsG0180004495.01.T01:CDS |
| ATTCACAAACAAAGTTCAAA+GGG | 0.522384 | 1:+78869485 | None:intergenic |
| TTTGTGAATGCGATTCTGGC+TGG | 0.522677 | 1:-78869471 | MsG0180004495.01.T01:CDS |
| CATTCACAAACAAAGTTCAA+AGG | 0.535528 | 1:+78869484 | None:intergenic |
| CTACTTCCCATGGCTGCCAA+AGG | 0.546322 | 1:-78869783 | MsG0180004495.01.T01:CDS |
| TCTCATGTTACTACTTCCCA+TGG | 0.547836 | 1:-78869793 | MsG0180004495.01.T01:CDS |
| AGAAAAGGTTTGTGAAGAAG+TGG | 0.556344 | 1:-78869545 | MsG0180004495.01.T01:CDS |
| AAATCCCCTTTGGCAGCCAT+GGG | 0.560106 | 1:+78869777 | None:intergenic |
| GAACTGGAGGAGGTGCTGGT+TGG | 0.589415 | 1:+78869209 | None:intergenic |
| GAAGAAGTGGAGTGTGGGAA+AGG | 0.596466 | 1:-78869532 | MsG0180004495.01.T01:CDS |
| ACTTCCCATGGCTGCCAAAG+GGG | 0.616013 | 1:-78869781 | MsG0180004495.01.T01:CDS |
| AAAGTCTTCTCTGGAACTGG+AGG | 0.629011 | 1:+78869196 | None:intergenic |
| GTTTGTGAAGAAGTGGAGTG+TGG | 0.633621 | 1:-78869538 | MsG0180004495.01.T01:CDS |
| TTTGTGAAGAAGTGGAGTGT+GGG | 0.637331 | 1:-78869537 | MsG0180004495.01.T01:CDS |
| TTACATTGAGGAATGACACA+TGG | 0.645633 | 1:+78869400 | None:intergenic |
| GTTACACTTACGATCAAAAG+CGG | 0.661338 | 1:+78869159 | None:intergenic |
| AAGTAGTAACATGAGAACCA+TGG | 0.695164 | 1:+78869800 | None:intergenic |
| TCAAAAGCGGAAATATTGCG+AGG | 0.697244 | 1:+78869172 | None:intergenic |
| GTGTGGGAAAGGAAACTGTG+TGG | 0.765418 | 1:-78869521 | MsG0180004495.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAACTTAATTTTTTTTATGA+AGG | - | Chr1:78869677-78869696 | MsG0180004495.01.T01:intron | 10.0% |
| !! | TATATATGCATTAGATATAT+AGG | + | Chr1:78869284-78869303 | None:intergenic | 15.0% |
| !! | ATATCCATATATATATGTCA+CGG | + | Chr1:78869407-78869426 | None:intergenic | 20.0% |
| !! | ATATCTAATGCATATATACA+TGG | - | Chr1:78869286-78869305 | MsG0180004495.01.T01:intron | 20.0% |
| !! | TATTCTATCATTTATCTTGT+AGG | - | Chr1:78869731-78869750 | MsG0180004495.01.T01:intron | 20.0% |
| ! | AGAGATTGAAATTTACATTG+AGG | + | Chr1:78869596-78869615 | None:intergenic | 25.0% |
| ! | ATATATCATCATCTTCATCT+TGG | + | Chr1:78869544-78869563 | None:intergenic | 25.0% |
| ! | ATTCACAAACAAAGTTCAAA+GGG | + | Chr1:78869499-78869518 | None:intergenic | 25.0% |
| ! | CAAGATAAATGATAGAATAG+AGG | + | Chr1:78869730-78869749 | None:intergenic | 25.0% |
| ! | CAAGTAAATAAGAGTTAGTA+AGG | + | Chr1:78869625-78869644 | None:intergenic | 25.0% |
| ! | GGATATATTAATTGCAGAAA+AGG | - | Chr1:78869421-78869440 | MsG0180004495.01.T01:CDS | 25.0% |
| ! | TATATCATCATCTTCATCTT+GGG | + | Chr1:78869543-78869562 | None:intergenic | 25.0% |
| ! | TTAACCGTGACATATATATA+TGG | - | Chr1:78869400-78869419 | MsG0180004495.01.T01:intron | 25.0% |
| AAAAATGAGTTTACGTACCA+AGG | + | Chr1:78869242-78869261 | None:intergenic | 30.0% | |
| CATTCACAAACAAAGTTCAA+AGG | + | Chr1:78869500-78869519 | None:intergenic | 30.0% | |
| GTAAATAAGAGTTAGTAAGG+AGG | + | Chr1:78869622-78869641 | None:intergenic | 30.0% | |
| TGATAGAATAGAGGAGATTT+AGG | + | Chr1:78869721-78869740 | None:intergenic | 30.0% | |
| AAGTAGTAACATGAGAACCA+TGG | + | Chr1:78869184-78869203 | None:intergenic | 35.0% | |
| AGAAAAGGTTTGTGAAGAAG+TGG | - | Chr1:78869436-78869455 | MsG0180004495.01.T01:CDS | 35.0% | |
| ATCATCAACAAAATCCCCTT+TGG | + | Chr1:78869217-78869236 | None:intergenic | 35.0% | |
| CTTGTAGGTAGCTTGAATTA+TGG | - | Chr1:78869746-78869765 | MsG0180004495.01.T01:intron | 35.0% | |
| GTTACACTTACGATCAAAAG+CGG | + | Chr1:78869825-78869844 | None:intergenic | 35.0% | |
| TTACATTGAGGAATGACACA+TGG | + | Chr1:78869584-78869603 | None:intergenic | 35.0% | |
| TTTGTTTGTGAATGCGATTC+TGG | - | Chr1:78869506-78869525 | MsG0180004495.01.T01:CDS | 35.0% | |
| !! | ATGGGTTCTTTCAAGTTGTT+GGG | - | Chr1:78869150-78869169 | MsG0180004495.01.T01:CDS | 35.0% |
| !!! | GATTTTGTTGATGATGTCCT+TGG | - | Chr1:78869222-78869241 | MsG0180004495.01.T01:CDS | 35.0% |
| TCAAAAGCGGAAATATTGCG+AGG | + | Chr1:78869812-78869831 | None:intergenic | 40.0% | |
| TCTCATGTTACTACTTCCCA+TGG | - | Chr1:78869188-78869207 | MsG0180004495.01.T01:CDS | 40.0% | |
| TTTGTGAAGAAGTGGAGTGT+GGG | - | Chr1:78869444-78869463 | MsG0180004495.01.T01:CDS | 40.0% | |
| GTTTGTGAAGAAGTGGAGTG+TGG | - | Chr1:78869443-78869462 | MsG0180004495.01.T01:CDS | 45.0% | |
| TTGCGAGGTAAAGTCTTCTC+TGG | + | Chr1:78869797-78869816 | None:intergenic | 45.0% | |
| TTTGTGAATGCGATTCTGGC+TGG | - | Chr1:78869510-78869529 | MsG0180004495.01.T01:CDS | 45.0% | |
| ! | GTTGTTGGGTCTTATAGCCA+TGG | - | Chr1:78869164-78869183 | MsG0180004495.01.T01:CDS | 45.0% |
| !! | AAAGTCTTCTCTGGAACTGG+AGG | + | Chr1:78869788-78869807 | None:intergenic | 45.0% |
| !! | GGTAAAGTCTTCTCTGGAAC+TGG | + | Chr1:78869791-78869810 | None:intergenic | 45.0% |
| !! | AAATAAATATATAAGAATTA+AGG | - | Chr1:78869651-78869670 | MsG0180004495.01.T01:intron | 5.0% |
| AAAATCCCCTTTGGCAGCCA+TGG | + | Chr1:78869208-78869227 | None:intergenic | 50.0% | |
| AAATCCCCTTTGGCAGCCAT+GGG | + | Chr1:78869207-78869226 | None:intergenic | 50.0% | |
| GAAGAAGTGGAGTGTGGGAA+AGG | - | Chr1:78869449-78869468 | MsG0180004495.01.T01:CDS | 50.0% | |
| GTGTGGGAAAGGAAACTGTG+TGG | - | Chr1:78869460-78869479 | MsG0180004495.01.T01:CDS | 50.0% | |
| TACTTCCCATGGCTGCCAAA+GGG | - | Chr1:78869199-78869218 | MsG0180004495.01.T01:CDS | 50.0% | |
| ACTTCCCATGGCTGCCAAAG+GGG | - | Chr1:78869200-78869219 | MsG0180004495.01.T01:CDS | 55.0% | |
| CTACTTCCCATGGCTGCCAA+AGG | - | Chr1:78869198-78869217 | MsG0180004495.01.T01:CDS | 55.0% | |
| !! | GTCTTCTCTGGAACTGGAGG+AGG | + | Chr1:78869785-78869804 | None:intergenic | 55.0% |
| ! | TCTGGAACTGGAGGAGGTGC+TGG | + | Chr1:78869779-78869798 | None:intergenic | 60.0% |
| !! | GAACTGGAGGAGGTGCTGGT+TGG | + | Chr1:78869775-78869794 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 78869150 | 78869853 | 78869150 | ID=MsG0180004495.01;Name=MsG0180004495.01 |
| Chr1 | mRNA | 78869150 | 78869853 | 78869150 | ID=MsG0180004495.01.T01;Parent=MsG0180004495.01;Name=MsG0180004495.01.T01;_AED=0.48;_eAED=0.51;_QI=0|0.5|0|1|1|1|3|0|118 |
| Chr1 | exon | 78869760 | 78869853 | 78869760 | ID=MsG0180004495.01.T01:exon:13049;Parent=MsG0180004495.01.T01 |
| Chr1 | exon | 78869404 | 78869565 | 78869404 | ID=MsG0180004495.01.T01:exon:13048;Parent=MsG0180004495.01.T01 |
| Chr1 | exon | 78869150 | 78869250 | 78869150 | ID=MsG0180004495.01.T01:exon:13047;Parent=MsG0180004495.01.T01 |
| Chr1 | CDS | 78869760 | 78869853 | 78869760 | ID=MsG0180004495.01.T01:cds;Parent=MsG0180004495.01.T01 |
| Chr1 | CDS | 78869404 | 78869565 | 78869404 | ID=MsG0180004495.01.T01:cds;Parent=MsG0180004495.01.T01 |
| Chr1 | CDS | 78869150 | 78869250 | 78869150 | ID=MsG0180004495.01.T01:cds;Parent=MsG0180004495.01.T01 |
| Gene Sequence |
| Protein sequence |
