AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039622.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039622.01.T01 MTR_7g076200 96.250 80 3 0 10 89 147 226 1.34e-49 159
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039622.01.T01 AT1G01820 72.500 80 22 0 10 89 147 226 1.79e-36 126
MsG0780039622.01.T01 AT2G45740 70.000 80 24 0 10 89 148 227 1.54e-35 123
MsG0780039622.01.T01 AT2G45740 70.000 80 24 0 10 89 148 227 1.54e-35 123
MsG0780039622.01.T01 AT2G45740 70.000 80 24 0 10 89 148 227 1.54e-35 123
MsG0780039622.01.T01 AT3G61070 62.500 80 26 1 10 89 147 222 2.86e-29 107
MsG0780039622.01.T01 AT3G61070 62.500 80 26 1 10 89 147 222 2.86e-29 107
MsG0780039622.01.T01 AT3G61070 62.500 80 26 1 10 89 147 222 2.86e-29 107

Find 26 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTCGGACAGCAGCTATTT+TGG 0.221592 7:+66792767 None:intergenic
CCCCGTGTTACCGGGGCATT+TGG 0.290157 7:-66792993 MsG0780039622.01.T01:CDS
ATCTCTAATATCTTGCTATC+AGG 0.317696 7:-66792961 MsG0780039622.01.T01:CDS
AAAGTCACTCCCCGTGTTAC+CGG 0.327800 7:-66793002 MsG0780039622.01.T01:CDS
TCGGGAGCTTGGAAGACTTC+TGG 0.372497 7:-66793277 MsG0780039622.01.T01:CDS
TTCTGATAGCTTTAGCAGCT+CGG 0.456319 7:+66792751 None:intergenic
AACACGGGGAGTGACTTTCT+CGG 0.476441 7:+66793007 None:intergenic
AAGTCACTCCCCGTGTTACC+GGG 0.483116 7:-66793001 MsG0780039622.01.T01:CDS
ATCCAAATGCCCCGGTAACA+CGG 0.484737 7:+66792991 None:intergenic
TCCAAATGCCCCGGTAACAC+GGG 0.487559 7:+66792992 None:intergenic
ACACGGGGAGTGACTTTCTC+GGG 0.492261 7:+66793008 None:intergenic
GTTTGATTAGCTCGGGAGCT+TGG 0.509994 7:-66793288 MsG0780039622.01.T01:CDS
TTTATGTGTTTGATTAGCTC+GGG 0.515748 7:-66793295 MsG0780039622.01.T01:CDS
TGCTACAGAGGGTAGCAGAT+GGG 0.538459 7:-66792827 MsG0780039622.01.T01:CDS
ACTCTTTCACTCATCAAAGC+AGG 0.538753 7:-66793071 MsG0780039622.01.T01:CDS
AGCAGTTGGACTGCTTCAGT+TGG 0.552071 7:-66793033 MsG0780039622.01.T01:CDS
TTGTCAATAGCGTGCTACAG+AGG 0.591440 7:-66792839 MsG0780039622.01.T01:CDS
TGAAACAAATCCAAATGCCC+CGG 0.597552 7:+66792983 None:intergenic
GTGCTACAGAGGGTAGCAGA+TGG 0.613154 7:-66792828 MsG0780039622.01.T01:CDS
ATTGATACAGTGGTAGCAGT+TGG 0.619234 7:-66793047 MsG0780039622.01.T01:CDS
GATGCAGAATGAGCAATACC+AGG 0.621633 7:-66793120 MsG0780039622.01.T01:intron
TGTCAATAGCGTGCTACAGA+GGG 0.622177 7:-66792838 MsG0780039622.01.T01:CDS
AGTCACTCCCCGTGTTACCG+GGG 0.628907 7:-66793000 MsG0780039622.01.T01:CDS
CAAAGCAGGCATTGATACAG+TGG 0.634472 7:-66793057 MsG0780039622.01.T01:CDS
CCAAATGCCCCGGTAACACG+GGG 0.648543 7:+66792993 None:intergenic
TAGCACGCTATTGACAACAC+TGG 0.707282 7:+66792845 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATGCAGATACAAAATTTTA+GGG + Chr7:66793149-66793168 None:intergenic 20.0%
! AGCTAAAAAAATCAAATGAG+AGG - Chr7:66792941-66792960 MsG0780039622.01.T01:CDS 25.0%
! ATTTCCTTACCTTTATCTTA+TGG - Chr7:66792893-66792912 MsG0780039622.01.T01:CDS 25.0%
!! GTATGCAGATACAAAATTTT+AGG + Chr7:66793150-66793169 None:intergenic 25.0%
!!! AAGTTGCTTCTTATTTTTCT+TGG - Chr7:66793120-66793139 MsG0780039622.01.T01:intron 25.0%
!!! CTCATTTGATTTTTTTAGCT+TGG + Chr7:66792941-66792960 None:intergenic 25.0%
ATTCTGCATCCATAAGATAA+AGG + Chr7:66792905-66792924 None:intergenic 30.0%
! ATCTCTAATATCTTGCTATC+AGG - Chr7:66793074-66793093 MsG0780039622.01.T01:CDS 30.0%
! TTCTGTCTTTGGTTTACTTT+GGG - Chr7:66792864-66792883 MsG0780039622.01.T01:CDS 30.0%
! TTTCTGTCTTTGGTTTACTT+TGG - Chr7:66792863-66792882 MsG0780039622.01.T01:CDS 30.0%
ATCTTCGAAGTTTCTGTCTT+TGG - Chr7:66792853-66792872 MsG0780039622.01.T01:CDS 35.0%
CGAAGATTTATACACATGCA+TGG + Chr7:66792840-66792859 None:intergenic 35.0%
GCATCCATAAGATAAAGGTA+AGG + Chr7:66792900-66792919 None:intergenic 35.0%
! TCTCTTTTCGCTAAAAGACA+CGG - Chr7:66793236-66793255 MsG0780039622.01.T01:CDS 35.0%
!!! TTGATTTTTTTAGCTTGGCC+TGG + Chr7:66792936-66792955 None:intergenic 35.0%
ACTCTTTCACTCATCAAAGC+AGG - Chr7:66792964-66792983 MsG0780039622.01.T01:CDS 40.0%
ATTGATACAGTGGTAGCAGT+TGG - Chr7:66792988-66793007 MsG0780039622.01.T01:CDS 40.0%
TGAAACAAATCCAAATGCCC+CGG + Chr7:66793055-66793074 None:intergenic 40.0%
!! TTCTGATAGCTTTAGCAGCT+CGG + Chr7:66793287-66793306 None:intergenic 40.0%
GATGCAGAATGAGCAATACC+AGG - Chr7:66792915-66792934 MsG0780039622.01.T01:CDS 45.0%
TAGCACGCTATTGACAACAC+TGG + Chr7:66793193-66793212 None:intergenic 45.0%
TGTCAATAGCGTGCTACAGA+GGG - Chr7:66793197-66793216 MsG0780039622.01.T01:intron 45.0%
TTGTCAATAGCGTGCTACAG+AGG - Chr7:66793196-66793215 MsG0780039622.01.T01:intron 45.0%
!! CAAAGCAGGCATTGATACAG+TGG - Chr7:66792978-66792997 MsG0780039622.01.T01:CDS 45.0%
AAAGTCACTCCCCGTGTTAC+CGG - Chr7:66793033-66793052 MsG0780039622.01.T01:CDS 50.0%
AACACGGGGAGTGACTTTCT+CGG + Chr7:66793031-66793050 None:intergenic 50.0%
AGCAGTTGGACTGCTTCAGT+TGG - Chr7:66793002-66793021 MsG0780039622.01.T01:CDS 50.0%
ATCCAAATGCCCCGGTAACA+CGG + Chr7:66793047-66793066 None:intergenic 50.0%
TGCTACAGAGGGTAGCAGAT+GGG - Chr7:66793208-66793227 MsG0780039622.01.T01:intron 50.0%
! AGCTCGGACAGCAGCTATTT+TGG + Chr7:66793271-66793290 None:intergenic 50.0%
! GTTTGATTAGCTCGGGAGCT+TGG - Chr7:66792747-66792766 MsG0780039622.01.T01:CDS 50.0%
AAGTCACTCCCCGTGTTACC+GGG - Chr7:66793034-66793053 MsG0780039622.01.T01:CDS 55.0%
ACACGGGGAGTGACTTTCTC+GGG + Chr7:66793030-66793049 None:intergenic 55.0%
GTGCTACAGAGGGTAGCAGA+TGG - Chr7:66793207-66793226 MsG0780039622.01.T01:intron 55.0%
TCCAAATGCCCCGGTAACAC+GGG + Chr7:66793046-66793065 None:intergenic 55.0%
TCGGGAGCTTGGAAGACTTC+TGG - Chr7:66792758-66792777 MsG0780039622.01.T01:CDS 55.0%
AGTCACTCCCCGTGTTACCG+GGG - Chr7:66793035-66793054 MsG0780039622.01.T01:CDS 60.0%
CCAAATGCCCCGGTAACACG+GGG + Chr7:66793045-66793064 None:intergenic 60.0%
! CCCCGTGTTACCGGGGCATT+TGG - Chr7:66793042-66793061 MsG0780039622.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 66792743 66793314 66792743 ID=MsG0780039622.01;Name=MsG0780039622.01
Chr7 mRNA 66792743 66793314 66792743 ID=MsG0780039622.01.T01;Parent=MsG0780039622.01;Name=MsG0780039622.01.T01;_AED=0.50;_eAED=0.73;_QI=0|1|0|1|1|1|2|0|159
Chr7 exon 66793228 66793314 66793228 ID=MsG0780039622.01.T01:exon:13259;Parent=MsG0780039622.01.T01
Chr7 exon 66792743 66793135 66792743 ID=MsG0780039622.01.T01:exon:13258;Parent=MsG0780039622.01.T01
Chr7 CDS 66793228 66793314 66793228 ID=MsG0780039622.01.T01:cds;Parent=MsG0780039622.01.T01
Chr7 CDS 66792743 66793135 66792743 ID=MsG0780039622.01.T01:cds;Parent=MsG0780039622.01.T01
Gene Sequence

>MsG0780039622.01.T01

ATGTGTTTGATTAGCTCGGGAGCTTGGAAGACTTCTGGATCAATGAAGAAGATAGAGAGAGAAATCAAGAACAGTAATAAATATGATAATGAGCAATACCAGGCCAAGCTAAAAAAATCAAATGAGAGGACTCTTTCACTCATCAAAGCAGGCATTGATACAGTGGTAGCAGTTGGACTGCTTCAGTTGGCACCCGAGAAAGTCACTCCCCGTGTTACCGGGGCATTTGGATTTGTTTCATCTCTAATATCTTGCTATCAGGTACTTTTACTGCTTGTATTATTAAAGTTGCTTCTTATTTTTCTTGGAGTCCCTAAAATTTTGTATCTGCATACTATGTGTACGCGCACACATACCAGTGTTGTCAATAGCGTGCTACAGAGGGTAGCAGATGGGAGCAGTCTCTTTTCGCTAAAAGACACGGCAGTAGCAGCCAAAATAGCTGCTGTCCGAGCTGCTAAAGCTATCAGAATGATATAA

Protein sequence

>MsG0780039622.01.T01

MCLISSGAWKTSGSMKKIEREIKNSNKYDNEQYQAKLKKSNERTLSLIKAGIDTVVAVGLLQLAPEKVTPRVTGAFGFVSSLISCYQVLLLLVLLKLLLIFLGVPKILYLHTMCTRTHTSVVNSVLQRVADGSSLFSLKDTAVAAKIAAVRAAKAIRMI*