AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005009.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005009.01.T01 MTR_4g056040 66.920 263 83 2 2 261 203 464 1.59e-114 339
MsG0180005009.01.T01 MTR_4g055990 62.671 292 99 4 2 285 210 499 6.80e-114 337
MsG0180005009.01.T01 MTR_4g056020 60.069 288 110 3 1 285 172 457 4.03e-111 329
MsG0180005009.01.T01 MTR_4g055990 64.207 271 88 3 2 264 210 479 5.94e-110 327
MsG0180005009.01.T01 MTR_1g090883 87.293 181 23 0 1 181 181 361 2.29e-108 319
MsG0180005009.01.T01 MTR_4g056030 59.431 281 109 3 2 279 173 451 8.14e-106 315
MsG0180005009.01.T01 MTR_7g110920 62.724 279 81 5 2 259 40 316 1.74e-105 310
MsG0180005009.01.T01 MTR_1g038710 65.299 268 89 3 2 266 187 453 2.49e-105 314
MsG0180005009.01.T01 MTR_4g055580 62.630 289 101 5 2 287 301 585 2.08e-104 316
MsG0180005009.01.T01 MTR_4g056070 65.044 226 78 1 2 227 213 437 4.91e-97 292
MsG0180005009.01.T01 MTR_4g056020 60.870 161 59 2 128 285 2 161 6.28e-62 193
MsG0180005009.01.T01 MTR_1g090890 86.420 81 11 0 181 261 33 113 4.10e-42 141
MsG0180005009.01.T01 MTR_4g093690 49.495 99 45 4 2 98 181 276 1.09e-13 70.1
MsG0180005009.01.T01 MTR_5g008950 35.514 107 62 3 141 243 233 336 5.80e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005009.01.T01 AT1G32375 27.509 269 164 12 2 263 168 412 4.42e-11 63.5
MsG0180005009.01.T01 AT1G32375 27.509 269 164 12 2 263 168 412 4.42e-11 63.5
MsG0180005009.01.T01 AT3G26930 26.016 246 162 9 3 239 169 403 6.26e-11 63.2
MsG0180005009.01.T01 AT3G26930 26.016 246 162 9 3 239 124 358 7.48e-11 62.8

Find 42 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTTTATACATGCCTTAA+TGG 0.130536 1:-85624521 None:intergenic
ACTTGAGGATCTTGCCATTA+AGG 0.236917 1:+85624507 MsG0180005009.01.T01:CDS
AAATCTTCCGTGTACAAGTA+TGG 0.262701 1:+85628108 MsG0180005009.01.T01:CDS
CTTCTTTCTACCGATTTATC+TGG 0.265237 1:-85628066 None:intergenic
ATGGCAAGATCCTCAAGTTT+TGG 0.290006 1:-85624502 None:intergenic
GGAGAGGCATCTCTATAAAA+AGG 0.318417 1:-85624044 None:intergenic
TGCCATTAAGGCATGTATAA+AGG 0.333413 1:+85624519 MsG0180005009.01.T01:CDS
GATTCTCACACCAGATAAAT+CGG 0.434058 1:+85628056 MsG0180005009.01.T01:CDS
TTCCTCAAGATGTGGACAAC+AGG 0.435338 1:-85623953 None:intergenic
GTGAAAGGAGACAAAATTGA+TGG 0.435767 1:-85624004 None:intergenic
TTCTAATGTCAAATCATTGA+CGG 0.438643 1:+85624248 MsG0180005009.01.T01:CDS
AAGACTAGCTTGATCAAGAT+TGG 0.443562 1:-85624172 None:intergenic
AAATATCTGAGAATTGTAGT+TGG 0.484238 1:+85624449 MsG0180005009.01.T01:CDS
AGTTCATGTCGACGTCTTCA+AGG 0.488620 1:+85628609 MsG0180005009.01.T01:CDS
TGAAAGACATGTTGGAGTTA+CGG 0.492083 1:-85627929 None:intergenic
TGTGAAAAGTGAGAAGATGT+AGG 0.495898 1:-85624405 None:intergenic
GAGAAAATCTAAAACAAAAG+AGG 0.499252 1:+85627879 MsG0180005009.01.T01:intron
TCAAAACATACAAACTGAGT+TGG 0.508227 1:+85627984 MsG0180005009.01.T01:CDS
TATCATGCAGCTTCTTAGCA+TGG 0.528074 1:+85624366 MsG0180005009.01.T01:intron
ATCCTGTTGTCCACATCTTG+AGG 0.529685 1:+85623951 MsG0180005009.01.T01:CDS
AAATCTAAAACAAAAGAGGT+TGG 0.529827 1:+85627883 MsG0180005009.01.T01:CDS
GTGAAAAGTGAGAAGATGTA+GGG 0.534578 1:-85624404 None:intergenic
AAGAGGTTGGAAACTCAGCT+TGG 0.535040 1:+85627896 MsG0180005009.01.T01:CDS
AATCTTCCGTGTACAAGTAT+GGG 0.536316 1:+85628109 MsG0180005009.01.T01:CDS
TCACTCAACTAAGTTAAAGA+AGG 0.541246 1:+85628029 MsG0180005009.01.T01:CDS
AGAGGTTGGAAACTCAGCTT+GGG 0.544035 1:+85627897 MsG0180005009.01.T01:CDS
GTGGACAAATGTCAGAATCA+AGG 0.550644 1:-85624065 None:intergenic
GGTTGGAAACTCAGCTTGGG+TGG 0.552779 1:+85627900 MsG0180005009.01.T01:CDS
TAATCTCTTCAATGAGTGAA+AGG 0.562935 1:-85624019 None:intergenic
AGACTGTTTGAAAGACATGT+TGG 0.565896 1:-85627937 None:intergenic
ACATGAAGATCTTCAAGCGA+AGG 0.578514 1:-85624102 None:intergenic
AGTTTGTATACACAATTGAA+AGG 0.591507 1:+85624222 MsG0180005009.01.T01:CDS
CATGAAGATCTTCAAGCGAA+GGG 0.591533 1:-85624101 None:intergenic
ACAGAATGCTCCAAAACTTG+AGG 0.601771 1:+85624492 MsG0180005009.01.T01:CDS
AAAGCAAATTCCTCAAGATG+TGG 0.606587 1:-85623961 None:intergenic
AGACATGTTGGAGTTACGGA+TGG 0.638903 1:-85627925 None:intergenic
GAAGGGGCATTAATTTGCAG+TGG 0.645272 1:-85624084 None:intergenic
TGAAAGGAGACAAAATTGAT+GGG 0.651423 1:-85624003 None:intergenic
ATGAAGATCTTCAAGCGAAG+GGG 0.669563 1:-85624100 None:intergenic
TTCATACCCATACTTGTACA+CGG 0.672812 1:-85628115 None:intergenic
CATGCAGCTTCTTAGCATGG+AGG 0.683407 1:+85624369 MsG0180005009.01.T01:intron
CAAATGTCAGAATCAAGGAG+AGG 0.696111 1:-85624060 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATGAAAAGAAAAAAAAA+AGG - Chr1:85625240-85625259 None:intergenic 10.0%
!! GTTATTATTAAAAAATAAGA+AGG - Chr1:85627694-85627713 None:intergenic 10.0%
!!! TTCTTTTAAATTCAAATTTA+AGG - Chr1:85627963-85627982 None:intergenic 10.0%
!!! TTTTTATTTGAATATATGAA+AGG + Chr1:85627088-85627107 MsG0180005009.01.T01:intron 10.0%
!! ATAATTATAGTAGTTTATTG+TGG - Chr1:85626093-85626112 None:intergenic 15.0%
!! TCCTTCAAAAAAAAATTTAT+TGG - Chr1:85626174-85626193 None:intergenic 15.0%
!! TGTCAAAAAAAAATGTATAA+TGG + Chr1:85626795-85626814 MsG0180005009.01.T01:intron 15.0%
!! TTAAGTCAATACAAAAAATT+TGG - Chr1:85625523-85625542 None:intergenic 15.0%
!! TTACTAACAAATTATATGAA+TGG - Chr1:85627727-85627746 None:intergenic 15.0%
!! TTTCAATTAGAAATATTGTT+AGG + Chr1:85628181-85628200 MsG0180005009.01.T01:intron 15.0%
!!! ACCAATAAATTTTTTTTTGA+AGG + Chr1:85626170-85626189 MsG0180005009.01.T01:intron 15.0%
!!! ATAAAAAAAAAAAGTGCATA+TGG - Chr1:85624349-85624368 None:intergenic 15.0%
!!! ATTCATATAATTTGTTAGTA+AGG + Chr1:85627726-85627745 MsG0180005009.01.T01:intron 15.0%
!!! TAAAAAAAAAAAGTGCATAT+GGG - Chr1:85624348-85624367 None:intergenic 15.0%
!!! TAATTATTCATATTTGTTAC+AGG + Chr1:85628569-85628588 MsG0180005009.01.T01:intron 15.0%
!!! TATATTTTTCTTTGAGAAAA+AGG - Chr1:85627498-85627517 None:intergenic 15.0%
!!! TTTGTACAATCTTTTTTAAA+AGG + Chr1:85624554-85624573 MsG0180005009.01.T01:intron 15.0%
!! AAAAATAAGAAGGTTTAACA+TGG - Chr1:85627684-85627703 None:intergenic 20.0%
!! AAAATTCCTAGTTGTATAAT+AGG - Chr1:85627389-85627408 None:intergenic 20.0%
!! AATTAAATGTTCTAGTAAGT+TGG - Chr1:85624899-85624918 None:intergenic 20.0%
!! ACTAAAAAGGAAAAAATGAT+AGG - Chr1:85625945-85625964 None:intergenic 20.0%
!! ATTCTTATAAATATGACTAG+AGG - Chr1:85624708-85624727 None:intergenic 20.0%
!! ATTCTTTCTTTACAAAGTTT+AGG - Chr1:85626302-85626321 None:intergenic 20.0%
!! CAACAAAACTTTGTATAATT+AGG - Chr1:85626749-85626768 None:intergenic 20.0%
!! CTGTTTGAAAAAGATAAAAA+GGG + Chr1:85626055-85626074 MsG0180005009.01.T01:intron 20.0%
!! GAAAAAATAATGTTTAACGA+AGG - Chr1:85626219-85626238 None:intergenic 20.0%
!! GAAAAGAAAAAAAAAAGGAT+AGG - Chr1:85625235-85625254 None:intergenic 20.0%
!! TACAAATATCATTTCTCATT+AGG - Chr1:85626558-85626577 None:intergenic 20.0%
!! TATAACTATGCAAAAACAAA+AGG + Chr1:85625666-85625685 MsG0180005009.01.T01:intron 20.0%
!! TCATAAAGTAGAAGAAAAAA+TGG - Chr1:85625915-85625934 None:intergenic 20.0%
!! TGTAAGGATAAAAACTAAAA+AGG - Chr1:85625958-85625977 None:intergenic 20.0%
!! TTACAAACAAAACAAAAAGA+TGG - Chr1:85627210-85627229 None:intergenic 20.0%
!! TTCTTATAAATATGACTAGA+GGG - Chr1:85624707-85624726 None:intergenic 20.0%
!! TTCTTATTGGATAAAAACAA+TGG - Chr1:85626664-85626683 None:intergenic 20.0%
!!! AAACTTGTTCATAATTGATA+TGG - Chr1:85628512-85628531 None:intergenic 20.0%
!!! AATTCGAAAATTTTCAAATG+TGG - Chr1:85624822-85624841 None:intergenic 20.0%
!!! AGAAGAAAAAATGGTTTTAT+TGG - Chr1:85625906-85625925 None:intergenic 20.0%
!!! AGTTTTAAATCAAGCTTTAT+AGG - Chr1:85625313-85625332 None:intergenic 20.0%
!!! ATTTTTTCTTCTACTTTATG+AGG + Chr1:85625914-85625933 MsG0180005009.01.T01:intron 20.0%
!!! CTAATTATACAAAGTTTTGT+TGG + Chr1:85626747-85626766 MsG0180005009.01.T01:intron 20.0%
!!! TTGAAAGAGTATAAACATTA+TGG - Chr1:85625111-85625130 None:intergenic 20.0%
!!! TTTAGGATAAGCATAATTTA+TGG - Chr1:85626942-85626961 None:intergenic 20.0%
!!! TTTATAAGCTCATATTTTCT+GGG - Chr1:85624147-85624166 None:intergenic 20.0%
! AAAAAGGAGAAGTTACAAAA+GGG - Chr1:85627482-85627501 None:intergenic 25.0%
! AAATATCTGAGAATTGTAGT+TGG + Chr1:85624449-85624468 MsG0180005009.01.T01:CDS 25.0%
! AACAGCTAATTAAATGCATT+AGG - Chr1:85624857-85624876 None:intergenic 25.0%
! AACATAAAATAAAGGCAAGA+AGG + Chr1:85625823-85625842 MsG0180005009.01.T01:intron 25.0%
! AAGAAATCAAGTAGAGTATA+AGG + Chr1:85627608-85627627 MsG0180005009.01.T01:intron 25.0%
! AAGACTTAGGAAAAGATATT+TGG + Chr1:85624617-85624636 MsG0180005009.01.T01:intron 25.0%
! ACATAAAATAAAGGCAAGAA+GGG + Chr1:85625824-85625843 MsG0180005009.01.T01:intron 25.0%
! AGGAATGTTAAAACTATGTA+AGG - Chr1:85625974-85625993 None:intergenic 25.0%
! AGTTTGTATACACAATTGAA+AGG + Chr1:85624222-85624241 MsG0180005009.01.T01:CDS 25.0%
! CAAAACACATTCAATCAAAA+AGG - Chr1:85625480-85625499 None:intergenic 25.0%
! CAAAACTTTGTATAATTAGG+AGG - Chr1:85626746-85626765 None:intergenic 25.0%
! CATGGATATCATTAAAACTA+CGG - Chr1:85627642-85627661 None:intergenic 25.0%
! GACAACTATACTATATTTGT+TGG + Chr1:85626817-85626836 MsG0180005009.01.T01:intron 25.0%
! GCTGTTTGAAAAAGATAAAA+AGG + Chr1:85626054-85626073 MsG0180005009.01.T01:intron 25.0%
! TAACTCAACTTTATCGAAAA+TGG + Chr1:85626020-85626039 MsG0180005009.01.T01:intron 25.0%
! TATCATGTTCTCAAAAAACA+TGG - Chr1:85627660-85627679 None:intergenic 25.0%
! TCTCTCATTAACTATAATTC+AGG + Chr1:85625386-85625405 MsG0180005009.01.T01:intron 25.0%
! TTCTAATGTCAAATCATTGA+CGG + Chr1:85624248-85624267 MsG0180005009.01.T01:CDS 25.0%
! TTTGCTAGGATTAGATATAA+AGG - Chr1:85624755-85624774 None:intergenic 25.0%
!! AAATCTAAAACAAAAGAGGT+TGG + Chr1:85627883-85627902 MsG0180005009.01.T01:CDS 25.0%
!! CTTTATAAGCTCATATTTTC+TGG - Chr1:85624148-85624167 None:intergenic 25.0%
!! GAGAAAATCTAAAACAAAAG+AGG + Chr1:85627879-85627898 MsG0180005009.01.T01:intron 25.0%
!! TTTTTATCCAATAAGAAGAG+AGG + Chr1:85626667-85626686 MsG0180005009.01.T01:intron 25.0%
!!! AAATATTTCTAGGGCAATAA+AGG + Chr1:85626440-85626459 MsG0180005009.01.T01:intron 25.0%
!!! ATATTTTTTTTTGGTGGTTG+TGG + Chr1:85626395-85626414 MsG0180005009.01.T01:intron 25.0%
!!! GTTAAGGTTTCATTTTGATT+AGG - Chr1:85624933-85624952 None:intergenic 25.0%
!!! TATTTTTTTTTGGTGGTTGT+GGG + Chr1:85626396-85626415 MsG0180005009.01.T01:intron 25.0%
!!! TGTTGGTGTTTTATGTATAA+TGG + Chr1:85626764-85626783 MsG0180005009.01.T01:intron 25.0%
AAACTCATCAAAATCAAAGC+TGG - Chr1:85625554-85625573 None:intergenic 30.0%
AACCTTTATACATGCCTTAA+TGG - Chr1:85624524-85624543 None:intergenic 30.0%
AACTCATCTGAACCATATAT+CGG - Chr1:85625771-85625790 None:intergenic 30.0%
AACTTTGTATAATTAGGAGG+AGG - Chr1:85626743-85626762 None:intergenic 30.0%
AATGTTAGTATGATGTTGTG+GGG + Chr1:85628244-85628263 MsG0180005009.01.T01:intron 30.0%
ACGAAGGAATACACAATTAT+TGG - Chr1:85626203-85626222 None:intergenic 30.0%
AGAATGTTAGTATGATGTTG+TGG + Chr1:85628242-85628261 MsG0180005009.01.T01:intron 30.0%
AGACAGTTTGAATTAACTGA+TGG - Chr1:85625191-85625210 None:intergenic 30.0%
AGCATAAGATGTAAAGACTT+AGG + Chr1:85624604-85624623 MsG0180005009.01.T01:intron 30.0%
AGCTAATTAAATGCATTAGG+AGG - Chr1:85624854-85624873 None:intergenic 30.0%
ATATGCATATATGATTGCAG+AGG + Chr1:85626326-85626345 MsG0180005009.01.T01:intron 30.0%
ATGAATTGAAGAGAACATGA+AGG + Chr1:85626494-85626513 MsG0180005009.01.T01:intron 30.0%
ATGGAAAATATACACATCAG+CGG - Chr1:85625092-85625111 None:intergenic 30.0%
ATTCCTAGTTGTATAATAGG+TGG - Chr1:85627386-85627405 None:intergenic 30.0%
CTACTTGATTTCTTTGCAAA+AGG - Chr1:85627602-85627621 None:intergenic 30.0%
CTATGCAAAAACAAAAGGAA+TGG + Chr1:85625671-85625690 MsG0180005009.01.T01:intron 30.0%
CTGTTTCACAAAAATGTTAG+AGG - Chr1:85625994-85626013 None:intergenic 30.0%
CTTGAGGGAACATAAAATAA+AGG + Chr1:85625815-85625834 MsG0180005009.01.T01:intron 30.0%
GAAAAAGGAGAAGTTACAAA+AGG - Chr1:85627483-85627502 None:intergenic 30.0%
GAATGTTAGTATGATGTTGT+GGG + Chr1:85628243-85628262 MsG0180005009.01.T01:intron 30.0%
GACAGTTTGAATTAACTGAT+GGG - Chr1:85625190-85625209 None:intergenic 30.0%
GAGAATTGATTGAGATGTAT+TGG - Chr1:85625360-85625379 None:intergenic 30.0%
GCTAATTAAATGCATTAGGA+GGG - Chr1:85624853-85624872 None:intergenic 30.0%
GGGAAGAAAAAAAAAACTGA+GGG - Chr1:85625430-85625449 None:intergenic 30.0%
TAATCTCTTCAATGAGTGAA+AGG - Chr1:85624022-85624041 None:intergenic 30.0%
TATAGTTCTCCATTGAATGT+TGG + Chr1:85625262-85625281 MsG0180005009.01.T01:intron 30.0%
TCAAAACATACAAACTGAGT+TGG + Chr1:85627984-85628003 MsG0180005009.01.T01:CDS 30.0%
TCACTCAACTAAGTTAAAGA+AGG + Chr1:85628029-85628048 MsG0180005009.01.T01:CDS 30.0%
TCTGATTACTTGACTAATTG+CGG + Chr1:85625146-85625165 MsG0180005009.01.T01:intron 30.0%
TGAAAGGAGACAAAATTGAT+GGG - Chr1:85624006-85624025 None:intergenic 30.0%
TGCATTTAATTAGCTGTTGT+TGG + Chr1:85624859-85624878 MsG0180005009.01.T01:intron 30.0%
TGGAAAGAATTGAGATATCA+AGG - Chr1:85625340-85625359 None:intergenic 30.0%
TGGGAAGAAAAAAAAAACTG+AGG - Chr1:85625431-85625450 None:intergenic 30.0%
TTAGGTTAAGATTGTTTGCT+AGG - Chr1:85624769-85624788 None:intergenic 30.0%
TTCAGTTATACAACTTTCCT+TGG + Chr1:85626841-85626860 MsG0180005009.01.T01:intron 30.0%
TTCCAAAAGTTGTCAAAAAG+TGG - Chr1:85626584-85626603 None:intergenic 30.0%
TTCTTCCTGTATTGTCTATA+TGG - Chr1:85627797-85627816 None:intergenic 30.0%
TTTGTCCATATAGACAATAC+AGG + Chr1:85627789-85627808 MsG0180005009.01.T01:intron 30.0%
! AAACCCTAATTCTACACTTT+TGG + Chr1:85624794-85624813 MsG0180005009.01.T01:intron 30.0%
! AGAATTAGGGTTTTGCAATT+AGG - Chr1:85624787-85624806 None:intergenic 30.0%
! GAGTTAGGGAAATATTTCTA+GGG + Chr1:85626431-85626450 MsG0180005009.01.T01:intron 30.0%
! TTGTGTTTTTCTCGCTTTAT+TGG + Chr1:85625611-85625630 MsG0180005009.01.T01:intron 30.0%
!! TTGTTGTTTGATTTGACACT+CGG - Chr1:85627458-85627477 None:intergenic 30.0%
!!! AACCACTTTTTGACAACTTT+TGG + Chr1:85626579-85626598 MsG0180005009.01.T01:intron 30.0%
!!! GGTTGTGGGATATTTTTTTT+TGG + Chr1:85626386-85626405 MsG0180005009.01.T01:intron 30.0%
!!! GGTTTTATTGGCAAAAGAAT+TGG - Chr1:85625894-85625913 None:intergenic 30.0%
!!! TGTGGGATATTTTTTTTTGG+TGG + Chr1:85626389-85626408 MsG0180005009.01.T01:intron 30.0%
!!! TGTTTTGTTTGTAAATGCTC+TGG + Chr1:85627216-85627235 MsG0180005009.01.T01:intron 30.0%
AAAGCAAATTCCTCAAGATG+TGG - Chr1:85623964-85623983 None:intergenic 35.0%
AAATCTTCCGTGTACAAGTA+TGG + Chr1:85628108-85628127 MsG0180005009.01.T01:CDS 35.0%
AAGGAATGGTAGTAACAAAG+TGG + Chr1:85625685-85625704 MsG0180005009.01.T01:intron 35.0%
AATCTTCCGTGTACAAGTAT+GGG + Chr1:85628109-85628128 MsG0180005009.01.T01:CDS 35.0%
AATGGTAGTAACAAAGTGGA+GGG + Chr1:85625689-85625708 MsG0180005009.01.T01:intron 35.0%
ACATGTGAGTATGATAGAGA+GGG - Chr1:85626610-85626629 None:intergenic 35.0%
AGACTGTTTGAAAGACATGT+TGG - Chr1:85627940-85627959 None:intergenic 35.0%
ATATACATAAGCGAGAAGCA+CGG - Chr1:85625588-85625607 None:intergenic 35.0%
ATATGCTTCCCTCAAAATCA+AGG + Chr1:85627515-85627534 MsG0180005009.01.T01:intron 35.0%
ATTGAAGAGAACATGAAGGT+TGG + Chr1:85626498-85626517 MsG0180005009.01.T01:intron 35.0%
CAATAAGAAGAGAGGAAAAG+TGG + Chr1:85626675-85626694 MsG0180005009.01.T01:intron 35.0%
CGAGTTAGGGAAATATTTCT+AGG + Chr1:85626430-85626449 MsG0180005009.01.T01:intron 35.0%
CTAGTTGTATAATAGGTGGT+GGG - Chr1:85627382-85627401 None:intergenic 35.0%
GATGATTGCATGATAGACTT+TGG - Chr1:85625026-85625045 None:intergenic 35.0%
GATTCTCACACCAGATAAAT+CGG + Chr1:85628056-85628075 MsG0180005009.01.T01:CDS 35.0%
GCAAAAGAATTGGATCTCAA+TGG - Chr1:85625884-85625903 None:intergenic 35.0%
GCAGAGGTGAAGAAAAAAAT+GGG - Chr1:85625450-85625469 None:intergenic 35.0%
GCATGATAGACTTTGGTATT+AGG - Chr1:85625019-85625038 None:intergenic 35.0%
GGATATTGTTGATCGAGTTA+GGG + Chr1:85626417-85626436 MsG0180005009.01.T01:intron 35.0%
GGCGATCATTGTATTACTAA+AGG - Chr1:85627545-85627564 None:intergenic 35.0%
GTGAAAAGTGAGAAGATGTA+GGG - Chr1:85624407-85624426 None:intergenic 35.0%
GTGAAAGGAGACAAAATTGA+TGG - Chr1:85624007-85624026 None:intergenic 35.0%
TAGATTGTTCGTAGATTGCA+TGG + Chr1:85626470-85626489 MsG0180005009.01.T01:intron 35.0%
TATGCTTCCCTCAAAATCAA+GGG + Chr1:85627516-85627535 MsG0180005009.01.T01:intron 35.0%
TATGTTCACTTCTACATTCG+TGG + Chr1:85628322-85628341 MsG0180005009.01.T01:intron 35.0%
TGAAAGACATGTTGGAGTTA+CGG - Chr1:85627932-85627951 None:intergenic 35.0%
TGAATTAACTGATGGGATAG+TGG - Chr1:85625183-85625202 None:intergenic 35.0%
TGCAGAGGTGAAGAAAAAAA+TGG - Chr1:85625451-85625470 None:intergenic 35.0%
TGCCATTAAGGCATGTATAA+AGG + Chr1:85624519-85624538 MsG0180005009.01.T01:CDS 35.0%
TGTGAAAAGTGAGAAGATGT+AGG - Chr1:85624408-85624427 None:intergenic 35.0%
TTCATACCCATACTTGTACA+CGG - Chr1:85628118-85628137 None:intergenic 35.0%
TTGCAAAAGGAGAGATTCAT+TGG - Chr1:85627589-85627608 None:intergenic 35.0%
! CACTTTTCCTCTCTTCTTAT+TGG - Chr1:85626677-85626696 None:intergenic 35.0%
! GAGAGATTCATTGGTGATAT+GGG - Chr1:85627580-85627599 None:intergenic 35.0%
! GAGCCAAAAGTGTAGAATTA+GGG - Chr1:85624800-85624819 None:intergenic 35.0%
! GTTTTTCTTCGATTACCTTC+AGG - Chr1:85626145-85626164 None:intergenic 35.0%
! TCACAAAAGTTGTCTAGGAT+TGG + Chr1:85626701-85626720 MsG0180005009.01.T01:intron 35.0%
!! AAGACTAGCTTGATCAAGAT+TGG - Chr1:85624175-85624194 None:intergenic 35.0%
!! CAACTAGGAATTTTGAAGAG+AGG + Chr1:85627395-85627414 MsG0180005009.01.T01:intron 35.0%
!! CTTCTTTCTACCGATTTATC+TGG - Chr1:85628069-85628088 None:intergenic 35.0%
!!! ATTTTTTTCTTCACCTCTGC+AGG + Chr1:85625450-85625469 MsG0180005009.01.T01:intron 35.0%
ACAGAATGCTCCAAAACTTG+AGG + Chr1:85624492-85624511 MsG0180005009.01.T01:CDS 40.0%
ACATGAAGATCTTCAAGCGA+AGG - Chr1:85624105-85624124 None:intergenic 40.0%
ACTTGAGGATCTTGCCATTA+AGG + Chr1:85624507-85624526 MsG0180005009.01.T01:CDS 40.0%
AGGCAAGAAGGGAAACTTAA+CGG + Chr1:85625835-85625854 MsG0180005009.01.T01:intron 40.0%
AGTTTAGGCAACTTGAAGCT+TGG - Chr1:85626287-85626306 None:intergenic 40.0%
ATGAAGATCTTCAAGCGAAG+GGG - Chr1:85624103-85624122 None:intergenic 40.0%
ATGCTTCCCTCAAAATCAAG+GGG + Chr1:85627517-85627536 MsG0180005009.01.T01:intron 40.0%
ATGGTAGTAACAAAGTGGAG+GGG + Chr1:85625690-85625709 MsG0180005009.01.T01:intron 40.0%
ATTGTAGCTAGTAGCTACCA+AGG - Chr1:85626861-85626880 None:intergenic 40.0%
CAAATGTCAGAATCAAGGAG+AGG - Chr1:85624063-85624082 None:intergenic 40.0%
CAACTTATAAGCCCAGAAAG+TGG - Chr1:85625794-85625813 None:intergenic 40.0%
CACATGTGAGTATGATAGAG+AGG - Chr1:85626611-85626630 None:intergenic 40.0%
CACGCATATATATAGCAGCA+TGG - Chr1:85624968-85624987 None:intergenic 40.0%
CATGAAGATCTTCAAGCGAA+GGG - Chr1:85624104-85624123 None:intergenic 40.0%
CATTCGTGGTTGAGTACTAT+AGG + Chr1:85628336-85628355 MsG0180005009.01.T01:intron 40.0%
CCACCACCTATTATACAACT+AGG + Chr1:85627380-85627399 MsG0180005009.01.T01:intron 40.0%
CCTAGTTGTATAATAGGTGG+TGG - Chr1:85627383-85627402 None:intergenic 40.0%
CTGTACTCAGATGTAGTTCT+TGG + Chr1:85625634-85625653 MsG0180005009.01.T01:intron 40.0%
GAATGGTAGTAACAAAGTGG+AGG + Chr1:85625688-85625707 MsG0180005009.01.T01:intron 40.0%
GACTTTGGTATTAGGCTCAT+TGG - Chr1:85625011-85625030 None:intergenic 40.0%
GCTTTCTTTCCTCCGATATA+TGG + Chr1:85625756-85625775 MsG0180005009.01.T01:intron 40.0%
GGGATATTGTTGATCGAGTT+AGG + Chr1:85626416-85626435 MsG0180005009.01.T01:intron 40.0%
GGTTGTCACAAAAGTTGTCT+AGG + Chr1:85626696-85626715 MsG0180005009.01.T01:intron 40.0%
GTGGACAAATGTCAGAATCA+AGG - Chr1:85624068-85624087 None:intergenic 40.0%
TATCATGCAGCTTCTTAGCA+TGG + Chr1:85624366-85624385 MsG0180005009.01.T01:intron 40.0%
TCAAAAAGGAAATCCTGCAG+AGG - Chr1:85625466-85625485 None:intergenic 40.0%
TCAGATGAGTTCCACTTTCT+GGG + Chr1:85625780-85625799 MsG0180005009.01.T01:intron 40.0%
TCATCTGAACCATATATCGG+AGG - Chr1:85625768-85625787 None:intergenic 40.0%
TCGACACAACCAACATTCAA+TGG - Chr1:85625274-85625293 None:intergenic 40.0%
TGACTAATTGCGGAGTACAT+TGG + Chr1:85625156-85625175 MsG0180005009.01.T01:intron 40.0%
TGCATATATGATTGCAGAGG+AGG + Chr1:85626329-85626348 MsG0180005009.01.T01:intron 40.0%
TTCAGATGAGTTCCACTTTC+TGG + Chr1:85625779-85625798 MsG0180005009.01.T01:intron 40.0%
! ATGGCAAGATCCTCAAGTTT+TGG - Chr1:85624505-85624524 None:intergenic 40.0%
! CATTGGTGATATGGGAACTT+GGG - Chr1:85627572-85627591 None:intergenic 40.0%
! GGAGAGATTCATTGGTGATA+TGG - Chr1:85627581-85627600 None:intergenic 40.0%
! GGAGCCAAAAGTGTAGAATT+AGG - Chr1:85624801-85624820 None:intergenic 40.0%
! TCATTGGTGATATGGGAACT+TGG - Chr1:85627573-85627592 None:intergenic 40.0%
! TGATTTGACACTCGGTTTTG+TGG - Chr1:85627450-85627469 None:intergenic 40.0%
!! GGAGAGGCATCTCTATAAAA+AGG - Chr1:85624047-85624066 None:intergenic 40.0%
!! GGGCTTATAAGTTGACTTGA+GGG + Chr1:85625800-85625819 MsG0180005009.01.T01:intron 40.0%
!! TGGGCTTATAAGTTGACTTG+AGG + Chr1:85625799-85625818 MsG0180005009.01.T01:intron 40.0%
AAAAGTGAACGTCTCTGCCA+AGG - Chr1:85626363-85626382 None:intergenic 45.0%
AGACATGTTGGAGTTACGGA+TGG - Chr1:85627928-85627947 None:intergenic 45.0%
AGTTCATGTCGACGTCTTCA+AGG + Chr1:85628609-85628628 MsG0180005009.01.T01:CDS 45.0%
ATCCTGTTGTCCACATCTTG+AGG + Chr1:85623951-85623970 MsG0180005009.01.T01:CDS 45.0%
ATGGCACACATCAGCTGTTA+AGG - Chr1:85624949-85624968 None:intergenic 45.0%
CAGAGGAGGATAAATTGCCT+TGG + Chr1:85626343-85626362 MsG0180005009.01.T01:intron 45.0%
TATACACATCAGCGGCTCAT+TGG - Chr1:85625084-85625103 None:intergenic 45.0%
TGCTTCCCTCAAAATCAAGG+GGG + Chr1:85627518-85627537 MsG0180005009.01.T01:intron 45.0%
TTCCTCAAGATGTGGACAAC+AGG - Chr1:85623956-85623975 None:intergenic 45.0%
TTGTGACAAGCTTCACCTGA+AGG + Chr1:85626127-85626146 MsG0180005009.01.T01:intron 45.0%
! AAAGGACCCCCTTGATTTTG+AGG - Chr1:85627527-85627546 None:intergenic 45.0%
! AAGGACCCCCTTGATTTTGA+GGG - Chr1:85627526-85627545 None:intergenic 45.0%
! TTGGCAGAGACGTTCACTTT+TGG + Chr1:85626362-85626381 MsG0180005009.01.T01:intron 45.0%
!! ACGTTCACTTTTGGTGGTTG+TGG + Chr1:85626371-85626390 MsG0180005009.01.T01:intron 45.0%
!! CGTTCACTTTTGGTGGTTGT+GGG + Chr1:85626372-85626391 MsG0180005009.01.T01:intron 45.0%
!! GAAGGGGCATTAATTTGCAG+TGG - Chr1:85624087-85624106 None:intergenic 45.0%
!!! TTTTGTAAATATTTTTTTTT+AGG - Chr1:85626959-85626978 None:intergenic 5.0%
AGTGGAGGGGATCAAGAATG+TGG + Chr1:85625703-85625722 MsG0180005009.01.T01:intron 50.0%
CATGCAGCTTCTTAGCATGG+AGG + Chr1:85624369-85624388 MsG0180005009.01.T01:intron 50.0%
GGTGATATGGGAACTTGGGA+GGG - Chr1:85627568-85627587 None:intergenic 50.0%
GTGATATGGGAACTTGGGAG+GGG - Chr1:85627567-85627586 None:intergenic 50.0%
TAATAGACCCGTGCATCGCA+CGG + Chr1:85627301-85627320 MsG0180005009.01.T01:intron 50.0%
TGATATGGGAACTTGGGAGG+GGG - Chr1:85627566-85627585 None:intergenic 50.0%
TGGTGATATGGGAACTTGGG+AGG - Chr1:85627569-85627588 None:intergenic 50.0%
TGTGTGTCGAGCATTAAGCG+AGG - Chr1:85628283-85628302 None:intergenic 50.0%
!! AAGGTTGGTCTGCAGCGTAT+TGG + Chr1:85626513-85626532 MsG0180005009.01.T01:intron 50.0%
!! GCAGAGACGTTCACTTTTGG+TGG + Chr1:85626365-85626384 MsG0180005009.01.T01:intron 50.0%
AATAGACCCGTGCATCGCAC+GGG + Chr1:85627302-85627321 MsG0180005009.01.T01:intron 55.0%
CTTCTTACCCGTGCGATGCA+CGG - Chr1:85627312-85627331 None:intergenic 55.0%
GAGGGGATCAAGAATGTGGC+AGG + Chr1:85625707-85625726 MsG0180005009.01.T01:intron 55.0%
TTCTTACCCGTGCGATGCAC+GGG - Chr1:85627311-85627330 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 85623913 85628660 85623913 ID=MsG0180005009.01;Name=MsG0180005009.01
Chr1 mRNA 85623913 85628660 85623913 ID=MsG0180005009.01.T01;Parent=MsG0180005009.01;Name=MsG0180005009.01.T01;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|1|1|4|0|287
Chr1 exon 85623913 85624290 85623913 ID=MsG0180005009.01.T01:exon:28237;Parent=MsG0180005009.01.T01
Chr1 exon 85624376 85624540 85624376 ID=MsG0180005009.01.T01:exon:28238;Parent=MsG0180005009.01.T01
Chr1 exon 85627880 85628130 85627880 ID=MsG0180005009.01.T01:exon:28239;Parent=MsG0180005009.01.T01
Chr1 exon 85628591 85628660 85628591 ID=MsG0180005009.01.T01:exon:28240;Parent=MsG0180005009.01.T01
Chr1 CDS 85623913 85624290 85623913 ID=MsG0180005009.01.T01:cds;Parent=MsG0180005009.01.T01
Chr1 CDS 85624376 85624540 85624376 ID=MsG0180005009.01.T01:cds;Parent=MsG0180005009.01.T01
Chr1 CDS 85627880 85628130 85627880 ID=MsG0180005009.01.T01:cds;Parent=MsG0180005009.01.T01
Chr1 CDS 85628591 85628660 85628591 ID=MsG0180005009.01.T01:cds;Parent=MsG0180005009.01.T01
Gene Sequence

>MsG0180005009.01.T01

ATGGATGATCATATTGAGTATGATCTCTCTAATTTTTTATCCTGTTGTCCACATCTTGAGGAATTTGCTTTACATGACTTCTTCAAACAACCCATCAATTTTGTCTCCTTTCACTCATTGAAGAGATTATACCTTTTTATAGAGATGCCTCTCCTTGATTCTGACATTTGTCCACTGCAAATTAATGCCCCTTCGCTTGAAGATCTTCATGTTGTTGATACATCATTGACTCCCAGAAAATATGAGCTTATAAAGTTGTCCAATCTTGATCAAGCTAGTCTTCACATTGTTAAGTATTCAGATTTTAACAGTTTGTATACACAATTGAAAGGACTTTCTAATGTCAAATCATTGACGGTCAAATCTGAAGCTATTCATCTTCTTAGCATGGAGGACAATCTTGATGCCCTACATCTTCTCACTTTTCACAATTTATTATTTTTATCGATTGAAATATCTGAGAATTGTAGTTGGAATATGCTCGTTAGCTTTATACAGAATGCTCCAAAACTTGAGGATCTTGCCATTAAGGCATGTATAAAGAGAAAATCTAAAACAAAAGAGGTTGGAAACTCAGCTTGGGTGGAGCCATCCGTAACTCCAACATGTCTTTCAAACAGTCTCCTTAAATTTGAATTTAAAAGAATTCAAAACATACAAACTGAGTTGGACTTTGCACAATATATTATTAATCACTCAACTAAGTTAAAGAAGGTAAAGATTCTCACACCAGATAAATCGGTAGAAAGAAGTTTTCACAAGATGTCAAAGAAATCTTCCGTGTACAAGTATGGGATATTAAATCAATTTGAAGTTCATGTCGACGTCTTCAAGGATTCAATCAAGTCTACTCGACACTTCTAA
Protein sequence

>MsG0180005009.01.T01

MDDHIEYDLSNFLSCCPHLEEFALHDFFKQPINFVSFHSLKRLYLFIEMPLLDSDICPLQINAPSLEDLHVVDTSLTPRKYELIKLSNLDQASLHIVKYSDFNSLYTQLKGLSNVKSLTVKSEAIHLLSMEDNLDALHLLTFHNLLFLSIEISENCSWNMLVSFIQNAPKLEDLAIKACIKRKSKTKEVGNSAWVEPSVTPTCLSNSLLKFEFKRIQNIQTELDFAQYIINHSTKLKKVKILTPDKSVERSFHKMSKKSSVYKYGILNQFEVHVDVFKDSIKSTRHF*