AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010904.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010904.01.T01 MTR_2g094160 97.101 69 2 0 1 69 1 69 1.44e-39 138
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010904.01.T01 AT5G60600 56.522 69 30 0 1 69 1 69 3.68e-21 85.9
MsG0280010904.01.T01 AT5G60600 56.522 69 30 0 1 69 1 69 3.68e-21 85.9
MsG0280010904.01.T01 AT5G60600 56.522 69 30 0 1 69 1 69 3.68e-21 85.9
MsG0280010904.01.T01 AT5G60600 56.522 69 30 0 1 69 1 69 3.84e-21 85.9
MsG0280010904.01.T01 AT5G60600 56.522 69 30 0 1 69 1 69 3.84e-21 85.9

Find 19 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGACATGTGATTCTGGTTT+GGG 0.212043 2:+76598578 MsG0280010904.01.T01:CDS
GTGATTCTGGTTTGGGGTTT+GGG 0.229538 2:+76598585 MsG0280010904.01.T01:CDS
TGTGATTCTGGTTTGGGGTT+TGG 0.230302 2:+76598584 MsG0280010904.01.T01:CDS
ACCCAAAAGTTGACTTCCTT+TGG 0.298868 2:-76598726 None:intergenic
ATCCAAAGGAAGTCAACTTT+TGG 0.309626 2:+76598724 MsG0280010904.01.T01:CDS
CAGGACATGTGATTCTGGTT+TGG 0.324391 2:+76598577 MsG0280010904.01.T01:CDS
AGCCTCAGGACATGTGATTC+TGG 0.355255 2:+76598572 MsG0280010904.01.T01:CDS
GGAGGAAGGTGTCAATTATC+AGG 0.388891 2:+76598660 MsG0280010904.01.T01:CDS
AGTTGACTTCCTTTGGATGC+AGG 0.455190 2:-76598719 None:intergenic
TGCAAAGAATGAAATCTTCA+AGG 0.459544 2:+76598639 MsG0280010904.01.T01:CDS
CAGGAATTCAAATCCTAGTC+AGG 0.472746 2:+76598679 MsG0280010904.01.T01:CDS
AGAATCACATGTCCTGAGGC+TGG 0.521171 2:-76598570 None:intergenic
GGACATGTGATTCTGGTTTG+GGG 0.553233 2:+76598579 MsG0280010904.01.T01:CDS
CTGAGGCTGGAAAATGAAGC+AGG 0.555201 2:-76598557 None:intergenic
AATGAAATCTTCAAGGAGGA+AGG 0.569471 2:+76598646 MsG0280010904.01.T01:CDS
AGTTCAGCAATATCCTGACT+AGG 0.582064 2:-76598692 None:intergenic
GAACTTCAACCTGCATCCAA+AGG 0.587296 2:+76598710 MsG0280010904.01.T01:CDS
AACCAGAATCACATGTCCTG+AGG 0.672465 2:-76598574 None:intergenic
AAAGAATGAAATCTTCAAGG+AGG 0.702362 2:+76598642 MsG0280010904.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAGAATGAAATCTTCAAGG+AGG + Chr2:76598642-76598661 MsG0280010904.01.T01:CDS 30.0%
TGCAAAGAATGAAATCTTCA+AGG + Chr2:76598639-76598658 MsG0280010904.01.T01:CDS 30.0%
AATGAAATCTTCAAGGAGGA+AGG + Chr2:76598646-76598665 MsG0280010904.01.T01:CDS 35.0%
! ATCCAAAGGAAGTCAACTTT+TGG + Chr2:76598724-76598743 MsG0280010904.01.T01:CDS 35.0%
! TCCAAAGGAAGTCAACTTTT+GGG + Chr2:76598725-76598744 MsG0280010904.01.T01:CDS 35.0%
!!! CGCTTCAATTGTTTTGTTTG+TGG + Chr2:76598763-76598782 MsG0280010904.01.T01:CDS 35.0%
AGTTCAGCAATATCCTGACT+AGG - Chr2:76598695-76598714 None:intergenic 40.0%
CAGGAATTCAAATCCTAGTC+AGG + Chr2:76598679-76598698 MsG0280010904.01.T01:CDS 40.0%
! ACCCAAAAGTTGACTTCCTT+TGG - Chr2:76598729-76598748 None:intergenic 40.0%
!! AGGACATGTGATTCTGGTTT+GGG + Chr2:76598578-76598597 MsG0280010904.01.T01:CDS 40.0%
AACCAGAATCACATGTCCTG+AGG - Chr2:76598577-76598596 None:intergenic 45.0%
GAACTTCAACCTGCATCCAA+AGG + Chr2:76598710-76598729 MsG0280010904.01.T01:CDS 45.0%
! AGTTGACTTCCTTTGGATGC+AGG - Chr2:76598722-76598741 None:intergenic 45.0%
! CAGGACATGTGATTCTGGTT+TGG + Chr2:76598577-76598596 MsG0280010904.01.T01:CDS 45.0%
!! GGACATGTGATTCTGGTTTG+GGG + Chr2:76598579-76598598 MsG0280010904.01.T01:CDS 45.0%
!! GGAGGAAGGTGTCAATTATC+AGG + Chr2:76598660-76598679 MsG0280010904.01.T01:CDS 45.0%
!! GTGATTCTGGTTTGGGGTTT+GGG + Chr2:76598585-76598604 MsG0280010904.01.T01:CDS 45.0%
!! TGTGATTCTGGTTTGGGGTT+TGG + Chr2:76598584-76598603 MsG0280010904.01.T01:CDS 45.0%
AGAATCACATGTCCTGAGGC+TGG - Chr2:76598573-76598592 None:intergenic 50.0%
AGCCTCAGGACATGTGATTC+TGG + Chr2:76598572-76598591 MsG0280010904.01.T01:CDS 50.0%
CTGAGGCTGGAAAATGAAGC+AGG - Chr2:76598560-76598579 None:intergenic 50.0%
! CTGCTTCATTTTCCAGCCTC+AGG + Chr2:76598558-76598577 MsG0280010904.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 76598539 76598790 76598539 ID=MsG0280010904.01;Name=MsG0280010904.01
Chr2 mRNA 76598539 76598790 76598539 ID=MsG0280010904.01.T01;Parent=MsG0280010904.01;Name=MsG0280010904.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|83
Chr2 exon 76598539 76598790 76598539 ID=MsG0280010904.01.T01:exon:27812;Parent=MsG0280010904.01.T01
Chr2 CDS 76598539 76598790 76598539 ID=MsG0280010904.01.T01:cds;Parent=MsG0280010904.01.T01
Gene Sequence

>MsG0280010904.01.T01

ATGGCTGCTGGAACTGTGCCTGCTTCATTTTCCAGCCTCAGGACATGTGATTCTGGTTTGGGGTTTGGGAAAAGTATTGATTTTGTGAGAATTTCTGATTTGCAAAGAATGAAATCTTCAAGGAGGAAGGTGTCAATTATCAGGAATTCAAATCCTAGTCAGGATATTGCTGAACTTCAACCTGCATCCAAAGGAAGTCAACTTTTGGGTATGAAAGTGTTTAACGCTTCAATTGTTTTGTTTGTGGATTAA

Protein sequence

>MsG0280010904.01.T01

MAAGTVPASFSSLRTCDSGLGFGKSIDFVRISDLQRMKSSRRKVSIIRNSNPSQDIAELQPASKGSQLLGMKVFNASIVLFVD*