AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041778.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041778.01.T01 MTR_7g117900 93.913 230 9 1 1 230 1 225 3.62e-155 429
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041778.01.T01 AT4G00895 40.945 127 61 3 65 187 75 191 2.88e-19 82.8
MsG0780041778.01.T01 AT4G09650 29.333 150 99 3 87 230 52 200 5.89e-14 69.3

Find 51 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAAGAGAAATGAGATTT+TGG 0.211304 7:+95056438 None:intergenic
ACTGAGTTTCTGCACACTTT+TGG 0.309140 7:+95055954 None:intergenic
TCAGTGGTGCAGTGAGGGTT+AGG 0.341774 7:-95055936 MsG0780041778.01.T01:CDS
ATGTGGAACTCAAGTTGTTT+TGG 0.349249 7:-95056022 MsG0780041778.01.T01:CDS
ATGGTTTGTGTTTGAGAGGT+TGG 0.358792 7:+95056349 None:intergenic
ATTGGTGAAGATGAAATGTT+TGG 0.381129 7:-95055982 MsG0780041778.01.T01:CDS
TGGTTTGTGTTTGAGAGGTT+GGG 0.403119 7:+95056350 None:intergenic
TTTGAGAGGTTGGGTCTTTG+AGG 0.403625 7:+95056359 None:intergenic
GCAGCAGCATAGCCAGTAGC+AGG 0.415721 7:+95056300 None:intergenic
TGAAGGGCATGTGGTTGGTT+TGG 0.422427 7:-95056124 MsG0780041778.01.T01:CDS
TGTTGGTGTTGACCCTTCAT+TGG 0.430284 7:-95056175 MsG0780041778.01.T01:CDS
TTGAGAGGTTGGGTCTTTGA+GGG 0.430753 7:+95056360 None:intergenic
ACCACCTTCCTCACCAATGA+AGG 0.445507 7:+95056162 None:intergenic
TTGTGAAGTGATAGAATTCA+CGG 0.447151 7:+95056502 None:intergenic
TTCCATCACAAGCCTGCTAC+TGG 0.468777 7:-95056312 MsG0780041778.01.T01:CDS
CACACTTCCCTCGGTTCAAA+GGG 0.473294 7:-95056250 MsG0780041778.01.T01:CDS
TTTGGTTGTGGAGGATGAGA+AGG 0.482489 7:+95056456 None:intergenic
ACACACTTCCCTCGGTTCAA+AGG 0.483662 7:-95056251 MsG0780041778.01.T01:CDS
TAGAATTCACGGTTGGTTGT+TGG 0.484183 7:+95056513 None:intergenic
TTGGTGAAGATGAAATGTTT+GGG 0.494512 7:-95055981 MsG0780041778.01.T01:CDS
CAGTGGTGCAGTGAGGGTTA+GGG 0.506987 7:-95055935 MsG0780041778.01.T01:CDS
AATTCACGGTTGGTTGTTGG+TGG 0.518266 7:+95056516 None:intergenic
TTGGTTGTGGAGGATGAGAA+GGG 0.520650 7:+95056457 None:intergenic
AGCCAGTAGCAGGCTTGTGA+TGG 0.522904 7:+95056310 None:intergenic
AGGATTTATGATGAATTATG+TGG 0.523605 7:-95056039 MsG0780041778.01.T01:CDS
GAAGATGGTTTGTGTTTGAG+AGG 0.530277 7:+95056345 None:intergenic
TGGAATACTTGAGAAGAAGA+TGG 0.533703 7:+95056330 None:intergenic
TTGAACCGAGGGAAGTGTGT+TGG 0.535631 7:+95056254 None:intergenic
AAATTTGAAGGGCATGTGGT+TGG 0.536206 7:-95056129 MsG0780041778.01.T01:CDS
AAGTGTTGGAAGAGTTCATG+AGG 0.536714 7:-95056059 MsG0780041778.01.T01:CDS
TGACCCTTCATTGGTGAGGA+AGG 0.538931 7:-95056166 MsG0780041778.01.T01:CDS
GTGGAGCAGAGAAAATTTGA+AGG 0.540648 7:-95056141 MsG0780041778.01.T01:CDS
GGGAATTGTTGAGGAAGTGT+TGG 0.544791 7:-95056073 MsG0780041778.01.T01:CDS
GCTGATGAAGAAGAACAAGC+TGG 0.546081 7:-95056094 MsG0780041778.01.T01:CDS
AAATTTCAATCAGTTGATGT+TGG 0.559351 7:-95056192 MsG0780041778.01.T01:CDS
GAAACTCAGTGGTGCAGTGA+GGG 0.562635 7:-95055941 MsG0780041778.01.T01:CDS
ATTCACGGTTGGTTGTTGGT+GGG 0.565174 7:+95056517 None:intergenic
TTCATTGGTGAGGAAGGTGG+TGG 0.567833 7:-95056160 MsG0780041778.01.T01:CDS
CCCTTCATTGGTGAGGAAGG+TGG 0.586166 7:-95056163 MsG0780041778.01.T01:CDS
CTGATGAAGAAGAACAAGCT+GGG 0.589597 7:-95056093 MsG0780041778.01.T01:CDS
GAGAAAATTTGAAGGGCATG+TGG 0.597790 7:-95056133 MsG0780041778.01.T01:CDS
TGAACATCCCTTTGAACCGA+GGG 0.602693 7:+95056243 None:intergenic
GTGTTGACCCTTCATTGGTG+AGG 0.604863 7:-95056170 MsG0780041778.01.T01:CDS
TTGAACATCCCTTTGAACCG+AGG 0.616637 7:+95056242 None:intergenic
GAAGGGTTTGCAGTTGCATG+AGG 0.616977 7:+95056474 None:intergenic
GAACAAGCTGGGAATTGTTG+AGG 0.625515 7:-95056082 MsG0780041778.01.T01:CDS
CCACCTTCCTCACCAATGAA+GGG 0.627070 7:+95056163 None:intergenic
GAAGTGATAGAATTCACGGT+TGG 0.666630 7:+95056506 None:intergenic
TGGAGCAGAGAAAATTTGAA+GGG 0.672649 7:-95056140 MsG0780041778.01.T01:CDS
AGAAACTCAGTGGTGCAGTG+AGG 0.695602 7:-95055942 MsG0780041778.01.T01:CDS
AAAAGTGTGCAGAAACTCAG+TGG 0.740335 7:-95055952 MsG0780041778.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAGAAAGTTTTTGTTTTTT+GGG + Chr7:95056036-95056055 None:intergenic 20.0%
! AGGATTTATGATGAATTATG+TGG - Chr7:95056401-95056420 MsG0780041778.01.T01:CDS 25.0%
! GTTTCATCAAAAAGAGAAAT+TGG - Chr7:95056440-95056459 MsG0780041778.01.T01:CDS 25.0%
!! AAATTTCAATCAGTTGATGT+TGG - Chr7:95056248-95056267 MsG0780041778.01.T01:CDS 25.0%
!! CTGATTGAAATTTCAGTTTT+TGG + Chr7:95056241-95056260 None:intergenic 25.0%
!!! ATTGAAATTTCAGTTTTTGG+AGG + Chr7:95056238-95056257 None:intergenic 25.0%
!!! GTGAGAAAGTTTTTGTTTTT+TGG + Chr7:95056037-95056056 None:intergenic 25.0%
ATTGGTGAAGATGAAATGTT+TGG - Chr7:95056458-95056477 MsG0780041778.01.T01:CDS 30.0%
TTGGTGAAGATGAAATGTTT+GGG - Chr7:95056459-95056478 MsG0780041778.01.T01:CDS 30.0%
TTGTGAAGTGATAGAATTCA+CGG + Chr7:95055941-95055960 None:intergenic 30.0%
! TTGGAAGAGAAATGAGATTT+TGG + Chr7:95056005-95056024 None:intergenic 30.0%
GAGGGTTAGGGTTAAAAATT+TGG - Chr7:95056517-95056536 MsG0780041778.01.T01:CDS 35.0%
TGGAATACTTGAGAAGAAGA+TGG + Chr7:95056113-95056132 None:intergenic 35.0%
TGGAGCAGAGAAAATTTGAA+GGG - Chr7:95056300-95056319 MsG0780041778.01.T01:CDS 35.0%
!! ATGTGGAACTCAAGTTGTTT+TGG - Chr7:95056418-95056437 MsG0780041778.01.T01:CDS 35.0%
!!! AAATGAGATTTTGGTTGTGG+AGG + Chr7:95055996-95056015 None:intergenic 35.0%
!!! GAGAAATGAGATTTTGGTTG+TGG + Chr7:95055999-95056018 None:intergenic 35.0%
!!! TGTTTTTTGGGTGACTAAGT+TGG + Chr7:95056024-95056043 None:intergenic 35.0%
AAAAACCAACACACTTCCCT+CGG - Chr7:95056181-95056200 MsG0780041778.01.T01:CDS 40.0%
CTGATGAAGAAGAACAAGCT+GGG - Chr7:95056347-95056366 MsG0780041778.01.T01:CDS 40.0%
GAAGTGATAGAATTCACGGT+TGG + Chr7:95055937-95055956 None:intergenic 40.0%
GTGGAGCAGAGAAAATTTGA+AGG - Chr7:95056299-95056318 MsG0780041778.01.T01:CDS 40.0%
! AAAAGTGTGCAGAAACTCAG+TGG - Chr7:95056488-95056507 MsG0780041778.01.T01:CDS 40.0%
! AAATTTGAAGGGCATGTGGT+TGG - Chr7:95056311-95056330 MsG0780041778.01.T01:CDS 40.0%
! ACTGAGTTTCTGCACACTTT+TGG + Chr7:95056489-95056508 None:intergenic 40.0%
! ATGGTTTGTGTTTGAGAGGT+TGG + Chr7:95056094-95056113 None:intergenic 40.0%
! GAAGATGGTTTGTGTTTGAG+AGG + Chr7:95056098-95056117 None:intergenic 40.0%
! GAGAAAATTTGAAGGGCATG+TGG - Chr7:95056307-95056326 MsG0780041778.01.T01:CDS 40.0%
! TAGAATTCACGGTTGGTTGT+TGG + Chr7:95055930-95055949 None:intergenic 40.0%
! TGGTTTGTGTTTGAGAGGTT+GGG + Chr7:95056093-95056112 None:intergenic 40.0%
!! AAGTGTTGGAAGAGTTCATG+AGG - Chr7:95056381-95056400 MsG0780041778.01.T01:CDS 40.0%
GAACAAGCTGGGAATTGTTG+AGG - Chr7:95056358-95056377 MsG0780041778.01.T01:CDS 45.0%
GCTGATGAAGAAGAACAAGC+TGG - Chr7:95056346-95056365 MsG0780041778.01.T01:CDS 45.0%
TGAACATCCCTTTGAACCGA+GGG + Chr7:95056200-95056219 None:intergenic 45.0%
TTGAACATCCCTTTGAACCG+AGG + Chr7:95056201-95056220 None:intergenic 45.0%
TTGAGAGGTTGGGTCTTTGA+GGG + Chr7:95056083-95056102 None:intergenic 45.0%
TTGGTTGTGGAGGATGAGAA+GGG + Chr7:95055986-95056005 None:intergenic 45.0%
TTTGAGAGGTTGGGTCTTTG+AGG + Chr7:95056084-95056103 None:intergenic 45.0%
TTTGGTTGTGGAGGATGAGA+AGG + Chr7:95055987-95056006 None:intergenic 45.0%
! AATTCACGGTTGGTTGTTGG+TGG + Chr7:95055927-95055946 None:intergenic 45.0%
! ATTCACGGTTGGTTGTTGGT+GGG + Chr7:95055926-95055945 None:intergenic 45.0%
! GGGAATTGTTGAGGAAGTGT+TGG - Chr7:95056367-95056386 MsG0780041778.01.T01:CDS 45.0%
!! GGTTGTTGGTGGGATTTTGA+GGG + Chr7:95055916-95055935 None:intergenic 45.0%
!! TGGTTGTTGGTGGGATTTTG+AGG + Chr7:95055917-95055936 None:intergenic 45.0%
!! TGTTGGTGTTGACCCTTCAT+TGG - Chr7:95056265-95056284 MsG0780041778.01.T01:CDS 45.0%
ACACACTTCCCTCGGTTCAA+AGG - Chr7:95056189-95056208 MsG0780041778.01.T01:CDS 50.0%
ACCACCTTCCTCACCAATGA+AGG + Chr7:95056281-95056300 None:intergenic 50.0%
AGAAACTCAGTGGTGCAGTG+AGG - Chr7:95056498-95056517 MsG0780041778.01.T01:CDS 50.0%
CACACTTCCCTCGGTTCAAA+GGG - Chr7:95056190-95056209 MsG0780041778.01.T01:CDS 50.0%
CCACCTTCCTCACCAATGAA+GGG + Chr7:95056280-95056299 None:intergenic 50.0%
GAAACTCAGTGGTGCAGTGA+GGG - Chr7:95056499-95056518 MsG0780041778.01.T01:CDS 50.0%
GAAGGGTTTGCAGTTGCATG+AGG + Chr7:95055969-95055988 None:intergenic 50.0%
TGACCCTTCATTGGTGAGGA+AGG - Chr7:95056274-95056293 MsG0780041778.01.T01:CDS 50.0%
TTCATTGGTGAGGAAGGTGG+TGG - Chr7:95056280-95056299 MsG0780041778.01.T01:CDS 50.0%
TTCCATCACAAGCCTGCTAC+TGG - Chr7:95056128-95056147 MsG0780041778.01.T01:CDS 50.0%
! GTGTTGACCCTTCATTGGTG+AGG - Chr7:95056270-95056289 MsG0780041778.01.T01:CDS 50.0%
! TGAAGGGCATGTGGTTGGTT+TGG - Chr7:95056316-95056335 MsG0780041778.01.T01:CDS 50.0%
! TTGAACCGAGGGAAGTGTGT+TGG + Chr7:95056189-95056208 None:intergenic 50.0%
AGCCAGTAGCAGGCTTGTGA+TGG + Chr7:95056133-95056152 None:intergenic 55.0%
CAGTGGTGCAGTGAGGGTTA+GGG - Chr7:95056505-95056524 MsG0780041778.01.T01:CDS 55.0%
CCCTTCATTGGTGAGGAAGG+TGG - Chr7:95056277-95056296 MsG0780041778.01.T01:CDS 55.0%
TCAGTGGTGCAGTGAGGGTT+AGG - Chr7:95056504-95056523 MsG0780041778.01.T01:CDS 55.0%
GCAGCAGCATAGCCAGTAGC+AGG + Chr7:95056143-95056162 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 95055885 95056577 95055885 ID=MsG0780041778.01;Name=MsG0780041778.01
Chr7 mRNA 95055885 95056577 95055885 ID=MsG0780041778.01.T01;Parent=MsG0780041778.01;Name=MsG0780041778.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|230
Chr7 exon 95055885 95056577 95055885 ID=MsG0780041778.01.T01:exon:20962;Parent=MsG0780041778.01.T01
Chr7 CDS 95055885 95056577 95055885 ID=MsG0780041778.01.T01:cds;Parent=MsG0780041778.01.T01
Gene Sequence

>MsG0780041778.01.T01

ATGGATGCTTTCTCAAGCTCTGTTTCAACCCTCAAAATCCCACCAACAACCAACCGTGAATTCTATCACTTCACAAGAACTCCTCATGCAACTGCAAACCCTTCTCATCCTCCACAACCAAAATCTCATTTCTCTTCCAACTTAGTCACCCAAAAAACAAAAACTTTCTCACACAACAATCATTCTACTTCACTACCCTCAAAGACCCAACCTCTCAAACACAAACCATCTTCTTCTCAAGTATTCCATCACAAGCCTGCTACTGGCTATGCTGCTGCAATCATAGATGTAGCTCAAAAAACCAACACACTTCCCTCGGTTCAAAGGGATGTTCAAAGACTCTTGAAGTTCCTCCAAAAACTGAAATTTCAATCAGTTGATGTTGGTGTTGACCCTTCATTGGTGAGGAAGGTGGTGGAGCAGAGAAAATTTGAAGGGCATGTGGTTGGTTTGGTGAAGATGCTGATGAAGAAGAACAAGCTGGGAATTGTTGAGGAAGTGTTGGAAGAGTTCATGAGGATTTATGATGAATTATGTGGAACTCAAGTTGTTTTGGTTTCATCAAAAAGAGAAATTGGTGAAGATGAAATGTTTGGGATTGCCAAAAGTGTGCAGAAACTCAGTGGTGCAGTGAGGGTTAGGGTTAAAAATTTGGTTCAAGAAGAGAGTTTTCCTTCTTCTTTTGCAGTGTAA

Protein sequence

>MsG0780041778.01.T01

MDAFSSSVSTLKIPPTTNREFYHFTRTPHATANPSHPPQPKSHFSSNLVTQKTKTFSHNNHSTSLPSKTQPLKHKPSSSQVFHHKPATGYAAAIIDVAQKTNTLPSVQRDVQRLLKFLQKLKFQSVDVGVDPSLVRKVVEQRKFEGHVVGLVKMLMKKNKLGIVEEVLEEFMRIYDELCGTQVVLVSSKREIGEDEMFGIAKSVQKLSGAVRVRVKNLVQEESFPSSFAV*