AlfalfaGEDB | MsG0680033862.01

M. sativa cultivar ZhongmuNo.1 / MsG0680033862.01

KEGG: -
GO: GO:0005515
PFAM: PF13041

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033862.01.T01	XP_003614163.1	98.246	171	3	0	1	171	490	660	6.89E-118	358
MsG0680033862.01.T02	PNX82214.1	98.507	67	1	0	1	67	62	128	8.42E-39	134

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033862.01.T01	P0C7R4	75.439	171	41	1	1	170	487	657	2.20E-93	287
MsG0680033862.01.T02	P0C7R4	81.818	66	12	0	1	66	592	657	1.24E-32	120

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033862.01.T01	G7JZ42	98.246	171	3	0	1	171	490	660	3.29e-118	358
MsG0680033862.01.T02	G7JY14	100.000	67	0	0	1	67	598	664	7.56e-37	139

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048660.01	MsG0680033862.01	0.802397	5.720235e-49	2.799249e-46
MsG0080048683.01	MsG0680033862.01	0.800713	1.266882e-48	5.939930e-46
MsG0080048826.01	MsG0680033862.01	0.801803	7.579104e-49	3.653039e-46
MsG0180000470.01	MsG0680033862.01	0.822644	2.121377e-53	1.786180e-50
MsG0180003951.01	MsG0680033862.01	0.811373	7.233148e-51	4.469665e-48
MsG0180004500.01	MsG0680033862.01	0.810988	8.763351e-51	5.361333e-48
MsG0180004765.01	MsG0680033862.01	0.811271	7.608662e-51	4.689044e-48
MsG0180006096.01	MsG0680033862.01	0.811801	5.838361e-51	3.648731e-48
MsG0280006309.01	MsG0680033862.01	0.810358	1.199603e-50	7.215671e-48
MsG0280010796.01	MsG0680033862.01	0.817014	4.106733e-52	2.956384e-49
MsG0280010916.01	MsG0680033862.01	0.801552	8.533809e-49	4.087055e-46
MsG0280010992.01	MsG0680033862.01	0.809771	1.604441e-50	9.504394e-48
MsG0280011217.01	MsG0680033862.01	0.807654	4.549306e-50	2.548064e-47
MsG0380012005.01	MsG0680033862.01	0.800678	1.287479e-48	6.031515e-46
MsG0680033862.01	MsG0680034230.01	0.813134	2.985003e-51	1.933155e-48
MsG0680033862.01	MsG0780036190.01	0.802828	4.661420e-49	2.306402e-46
MsG0680033862.01	MsG0780038439.01	0.804480	2.116838e-49	1.092609e-46
MsG0680033862.01	MsG0780039139.01	0.831725	1.418457e-55	1.552667e-52
MsG0680033862.01	MsG0780040763.01	0.802569	5.273022e-49	2.591512e-46
MsG0680033862.01	MsG0780041387.01	0.807875	4.081627e-50	2.299331e-47
MsG0680033862.01	MsG0780041416.01	0.807291	5.429371e-50	3.012608e-47
MsG0680033862.01	MsG0780041491.01	0.804242	2.372703e-49	1.217163e-46
MsG0680033862.01	MsG0780041673.01	0.836302	1.013427e-56	1.272116e-53
MsG0680033862.01	MsG0880041861.01	0.815609	8.468462e-52	5.866474e-49
MsG0680033862.01	MsG0880041863.01	0.810731	9.964473e-51	6.053772e-48
MsG0680033862.01	MsG0880041864.01	0.808571	2.900601e-50	1.664239e-47
MsG0680033862.01	MsG0880042022.01	0.818611	1.790430e-52	1.346558e-49
MsG0680033862.01	MsG0880043157.01	0.824633	7.260072e-54	6.467875e-51
MsG0680033862.01	MsG0880043298.01	0.800576	1.351045e-48	6.312886e-46
MsG0680033862.01	MsG0880043937.01	0.810108	1.358120e-50	8.116194e-48
MsG0680033862.01	MsG0880044036.01	0.805747	1.149624e-49	6.131047e-47
MsG0680033862.01	MsG0880045371.01	0.800931	1.143444e-48	5.391347e-46
MsG0680033862.01	MsG0880045765.01	0.823336	1.463551e-53	1.256465e-50
MsG0680033862.01	MsG0880045992.01	0.825802	3.843814e-54	3.540213e-51
MsG0680033862.01	MsG0880046699.01	0.828827	7.241538e-55	7.279277e-52
MsG0680033862.01	MsG0880047754.01	0.810886	9.220312e-51	5.625154e-48
MsG0480018653.01	MsG0680033862.01	0.819530	1.106567e-52	8.537706e-50
MsG0480020024.01	MsG0680033862.01	0.807049	6.110263e-50	3.369051e-47
MsG0480020887.01	MsG0680033862.01	0.829747	4.330404e-55	4.472845e-52
MsG0480021796.01	MsG0680033862.01	0.804687	1.916740e-49	9.946561e-47
MsG0480021869.01	MsG0680033862.01	0.811699	6.144827e-51	3.829743e-48
MsG0480022068.01	MsG0680033862.01	0.808535	2.952888e-50	1.692683e-47
MsG0480023488.01	MsG0680033862.01	0.804239	2.377014e-49	1.219273e-46
MsG0580024038.01	MsG0680033862.01	0.800917	1.150935e-48	5.424797e-46
MsG0580025832.01	MsG0680033862.01	0.811158	8.052869e-51	4.947929e-48
MsG0380014948.01	MsG0680033862.01	0.831330	1.774626e-55	1.919557e-52
MsG0380015277.01	MsG0680033862.01	0.802428	5.637174e-49	2.760785e-46
MsG0380015372.01	MsG0680033862.01	0.818533	1.865643e-52	1.400186e-49
MsG0380016285.01	MsG0680033862.01	0.809452	1.879574e-50	1.103924e-47
MsG0380017202.01	MsG0680033862.01	0.827448	1.555406e-54	1.502137e-51
MsG0380017244.01	MsG0680033862.01	0.801162	1.025659e-48	4.864278e-46
MsG0380017293.01	MsG0680033862.01	0.801856	7.389315e-49	3.566554e-46
MsG0380017931.01	MsG0680033862.01	0.803491	3.398774e-49	1.710320e-46
MsG0480018236.01	MsG0680033862.01	0.813864	2.062872e-51	1.362781e-48
MsG0580026122.01	MsG0680033862.01	0.822020	2.961896e-53	2.449537e-50
MsG0580026131.01	MsG0680033862.01	0.800531	1.379664e-48	6.439191e-46
MsG0580027922.01	MsG0680033862.01	0.817202	3.727446e-52	2.696997e-49
MsG0580029236.01	MsG0680033862.01	0.840775	7.096843e-58	1.021536e-54
MsG0580029925.01	MsG0680033862.01	0.813466	2.523403e-51	1.649237e-48
MsG0680030313.01	MsG0680033862.01	0.802542	5.340599e-49	2.622991e-46
MsG0280007575.01	MsG0680033862.01	0.810081	1.376026e-50	8.217060e-48
MsG0280007955.01	MsG0680033862.01	0.820637	6.175675e-53	4.912970e-50
MsG0280008076.01	MsG0680033862.01	0.808939	2.420933e-50	1.402482e-47
MsG0280010189.01	MsG0680033862.01	0.806347	8.595375e-50	4.654505e-47
MsG0280010350.01	MsG0680033862.01	0.808846	2.533464e-50	1.464103e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033862.01.T01	MTR_5g045620	98.246	171	3	0	1	171	490	660	8.34e-122	358
MsG0680033862.01.T01	MTR_5g044930	98.246	171	3	0	1	171	494	664	1.08e-121	357
MsG0680033862.01.T01	MTR_4g081540	36.207	174	103	4	1	169	615	785	4.45e-32	121
MsG0680033862.01.T01	MTR_4g099200	29.936	157	102	3	19	170	533	686	3.56e-22	92.8
MsG0680033862.01.T01	MTR_1g066900	29.775	178	112	3	5	169	524	701	9.17e-21	88.6
MsG0680033862.01.T02	MTR_5g044930	100.000	67	0	0	1	67	598	664	1.92e-40	139
MsG0680033862.01.T02	MTR_5g045620	100.000	67	0	0	1	67	594	660	2.16e-40	139

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033862.01.T01	AT1G69290	75.439	171	41	1	1	170	487	657	2.24e-94	287
MsG0680033862.01.T01	AT1G68980	64.072	167	53	1	1	167	456	615	1.55e-73	232
MsG0680033862.01.T01	AT1G03100	38.857	175	100	4	1	170	621	793	3.62e-35	130
MsG0680033862.01.T01	AT3G09650	32.967	182	103	3	6	169	582	762	4.18e-26	103
MsG0680033862.01.T01	AT4G17616	30.159	126	85	1	19	144	521	643	1.26e-16	77.0
MsG0680033862.01.T02	AT1G69290	81.818	66	12	0	1	66	592	657	1.26e-33	120
MsG0680033862.01.T02	AT1G68980	63.492	63	23	0	1	63	553	615	4.33e-23	90.9

Find 34 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTCATTCGGCTCGAATTGC+AGG	0.306666	6:-75127249	None:intergenic
CTGAAACATATCCGTTAATC+AGG	0.319880	6:-75127285	None:intergenic
TGAAAATATTTGTTGATAAG+TGG	0.364460	6:+75127482	MsG0680033862.01.T01:CDS
AGCTCAAAGTGGCCAGGTTA+AGG	0.380872	6:+75127539	MsG0680033862.01.T01:CDS
GGAATGCATCAATCAGATTC+TGG	0.383498	6:-75127393	None:intergenic
GACAAGTATGTCTGGTCATT+CGG	0.398593	6:-75127262	None:intergenic
GAGATACAAGCAAGCATTCA+TGG	0.427867	6:+75127504	MsG0680033862.01.T01:CDS
ACATACTTGTCCCTGATTAA+CGG	0.428702	6:+75127274	MsG0680033862.01.T01:CDS
TGGTCAAATTTGATCCCCTT+CGG	0.433100	6:-75127373	None:intergenic
GGCAGCTTTCTTAGATGAGT+TGG	0.449243	6:+75127114	MsG0680033862.01.T01:CDS
GAGGTTAAGAGAAAGTTAGC+AGG	0.460627	6:+75127343	MsG0680033862.01.T01:CDS
TTGAAGTGGGTACTCATGAT+TGG	0.463951	6:+75127152	MsG0680033862.01.T01:CDS
TTTCTTAGATGAGTTGGTTG+AGG	0.493444	6:+75127120	MsG0680033862.01.T01:CDS
AGGTTAAGAGAAAGTTAGCA+GGG	0.503999	6:+75127344	MsG0680033862.01.T01:CDS
TAATCAGGGACAAGTATGTC+TGG	0.515514	6:-75127270	None:intergenic
GTTAGCAGGGGATGGGCCGA+AGG	0.520097	6:+75127357	MsG0680033862.01.T01:CDS
AAGAGAAAGTTAGCAGGGGA+TGG	0.520299	6:+75127349	MsG0680033862.01.T01:CDS
TGCAATGATGTTGTGGAATG+AGG	0.521891	6:+75127324	MsG0680033862.01.T01:CDS
CTCCCTTGACCATAGCATAC+AGG	0.534135	6:-75127414	None:intergenic
TTAGCAGGGGATGGGCCGAA+GGG	0.566169	6:+75127358	MsG0680033862.01.T01:CDS
ATTCCTGTATGCTATGGTCA+AGG	0.570553	6:+75127411	MsG0680033862.01.T01:CDS
AGAGAAAGTTAGCAGGGGAT+GGG	0.579488	6:+75127350	MsG0680033862.01.T01:CDS
TGAGGATCCTCGTATTGAAG+TGG	0.582306	6:+75127138	MsG0680033862.01.T01:CDS
TTCCTGTATGCTATGGTCAA+GGG	0.590606	6:+75127412	MsG0680033862.01.T01:CDS
ATGCAAGAAGGACTTTCAGA+AGG	0.601668	6:+75127218	MsG0680033862.01.T01:CDS
TGATGCATTCCTGTATGCTA+TGG	0.602058	6:+75127405	MsG0680033862.01.T01:CDS
GGTTAAGAGAAAGTTAGCAG+GGG	0.610374	6:+75127345	MsG0680033862.01.T01:CDS
TGATGCTGTGATGCAAGTTG+TGG	0.610490	6:+75127444	MsG0680033862.01.T01:CDS
GGAGGCTAGAAGATGCAAGA+AGG	0.616733	6:+75127206	MsG0680033862.01.T01:CDS
TAGCAGGGGATGGGCCGAAG+GGG	0.624535	6:+75127359	MsG0680033862.01.T01:CDS
GAGGATCCTCGTATTGAAGT+GGG	0.632847	6:+75127139	MsG0680033862.01.T01:CDS
CTGTATGCTATGGTCAAGGG+AGG	0.634721	6:+75127415	MsG0680033862.01.T01:CDS
TGAAACATATCCGTTAATCA+GGG	0.644986	6:-75127284	None:intergenic
TGAGTACCCACTTCAATACG+AGG	0.733899	6:-75127145	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGAAAATATTTGTTGATAAG+TGG	+	Chr6:75127482-75127501	MsG0680033862.01.T01:CDS	20.0%
!	GAAAAAGAATGTCAGAAAAA+TGG	+	Chr6:75127561-75127580	MsG0680033862.01.T01:CDS	25.0%
!!!	GCTTATTGCTTTTAAGAATT+GGG	+	Chr6:75127588-75127607	MsG0680033862.01.T01:CDS	25.0%
	TGAAACATATCCGTTAATCA+GGG	-	Chr6:75127287-75127306	None:intergenic	30.0%
!!	ATTTTGATGCAATGATGTTG+TGG	+	Chr6:75127317-75127336	MsG0680033862.01.T01:CDS	30.0%
!!	CGCTTATTGCTTTTAAGAAT+TGG	+	Chr6:75127587-75127606	MsG0680033862.01.T01:CDS	30.0%
!!	TATAATCCACGCTTTTTGTA+AGG	+	Chr6:75127180-75127199	MsG0680033862.01.T01:CDS	30.0%
	ACATACTTGTCCCTGATTAA+CGG	+	Chr6:75127274-75127293	MsG0680033862.01.T01:CDS	35.0%
	AGGTTAAGAGAAAGTTAGCA+GGG	+	Chr6:75127344-75127363	MsG0680033862.01.T01:CDS	35.0%
	CTGAAACATATCCGTTAATC+AGG	-	Chr6:75127288-75127307	None:intergenic	35.0%
	GACACATAAAAAGCTCAAAG+TGG	+	Chr6:75127528-75127547	MsG0680033862.01.T01:CDS	35.0%
!	GACATTCTTTTTCCTTAACC+TGG	-	Chr6:75127554-75127573	None:intergenic	35.0%
!!	ATTGCTTTTAAGAATTGGGC+TGG	+	Chr6:75127592-75127611	MsG0680033862.01.T01:CDS	35.0%
!!	TTTCTTAGATGAGTTGGTTG+AGG	+	Chr6:75127120-75127139	MsG0680033862.01.T01:CDS	35.0%
	ATAAAAAGCTCAAAGTGGCC+AGG	+	Chr6:75127533-75127552	MsG0680033862.01.T01:CDS	40.0%
	ATGCAAGAAGGACTTTCAGA+AGG	+	Chr6:75127218-75127237	MsG0680033862.01.T01:CDS	40.0%
	ATTCCTGTATGCTATGGTCA+AGG	+	Chr6:75127411-75127430	MsG0680033862.01.T01:CDS	40.0%
	GACAAGTATGTCTGGTCATT+CGG	-	Chr6:75127265-75127284	None:intergenic	40.0%
	GAGATACAAGCAAGCATTCA+TGG	+	Chr6:75127504-75127523	MsG0680033862.01.T01:CDS	40.0%
	GAGGTTAAGAGAAAGTTAGC+AGG	+	Chr6:75127343-75127362	MsG0680033862.01.T01:CDS	40.0%
	GGAATGCATCAATCAGATTC+TGG	-	Chr6:75127396-75127415	None:intergenic	40.0%
	GGTTAAGAGAAAGTTAGCAG+GGG	+	Chr6:75127345-75127364	MsG0680033862.01.T01:CDS	40.0%
	TAATCAGGGACAAGTATGTC+TGG	-	Chr6:75127273-75127292	None:intergenic	40.0%
	TGATGCATTCCTGTATGCTA+TGG	+	Chr6:75127405-75127424	MsG0680033862.01.T01:CDS	40.0%
	TGCAATGATGTTGTGGAATG+AGG	+	Chr6:75127324-75127343	MsG0680033862.01.T01:CDS	40.0%
	TGGTCAAATTTGATCCCCTT+CGG	-	Chr6:75127376-75127395	None:intergenic	40.0%
	TTCCTGTATGCTATGGTCAA+GGG	+	Chr6:75127412-75127431	MsG0680033862.01.T01:CDS	40.0%
	TTGAAGTGGGTACTCATGAT+TGG	+	Chr6:75127152-75127171	MsG0680033862.01.T01:CDS	40.0%
	AAGAGAAAGTTAGCAGGGGA+TGG	+	Chr6:75127349-75127368	MsG0680033862.01.T01:CDS	45.0%
	AGAGAAAGTTAGCAGGGGAT+GGG	+	Chr6:75127350-75127369	MsG0680033862.01.T01:CDS	45.0%
	GAGGATCCTCGTATTGAAGT+GGG	+	Chr6:75127139-75127158	MsG0680033862.01.T01:CDS	45.0%
	GGCAGCTTTCTTAGATGAGT+TGG	+	Chr6:75127114-75127133	MsG0680033862.01.T01:CDS	45.0%
	TGAGGATCCTCGTATTGAAG+TGG	+	Chr6:75127138-75127157	MsG0680033862.01.T01:CDS	45.0%
	TGAGTACCCACTTCAATACG+AGG	-	Chr6:75127148-75127167	None:intergenic	45.0%
	TGATGCTGTGATGCAAGTTG+TGG	+	Chr6:75127444-75127463	MsG0680033862.01.T01:CDS	45.0%
!!	ATCCACGCTTTTTGTAAGGC+TGG	+	Chr6:75127184-75127203	MsG0680033862.01.T01:CDS	45.0%
!!	TCCACGCTTTTTGTAAGGCT+GGG	+	Chr6:75127185-75127204	MsG0680033862.01.T01:CDS	45.0%
	AGCTCAAAGTGGCCAGGTTA+AGG	+	Chr6:75127539-75127558	MsG0680033862.01.T01:CDS	50.0%
	CTCCCTTGACCATAGCATAC+AGG	-	Chr6:75127417-75127436	None:intergenic	50.0%
	CTGTATGCTATGGTCAAGGG+AGG	+	Chr6:75127415-75127434	MsG0680033862.01.T01:CDS	50.0%
	GGAGGCTAGAAGATGCAAGA+AGG	+	Chr6:75127206-75127225	MsG0680033862.01.T01:CDS	50.0%
	TCCCAGCCTTACAAAAAGCG+TGG	-	Chr6:75127189-75127208	None:intergenic	50.0%
!!	ACGCTTTTTGTAAGGCTGGG+AGG	+	Chr6:75127188-75127207	MsG0680033862.01.T01:CDS	50.0%
!	GGTCATTCGGCTCGAATTGC+AGG	-	Chr6:75127252-75127271	None:intergenic	55.0%
	TTAGCAGGGGATGGGCCGAA+GGG	+	Chr6:75127358-75127377	MsG0680033862.01.T01:CDS	60.0%
	GTTAGCAGGGGATGGGCCGA+AGG	+	Chr6:75127357-75127376	MsG0680033862.01.T01:CDS	65.0%
	TAGCAGGGGATGGGCCGAAG+GGG	+	Chr6:75127359-75127378	MsG0680033862.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	75127112	75127627	75127112	ID=MsG0680033862.01;Name=MsG0680033862.01
Chr6	mRNA	75127112	75127627	75127112	ID=MsG0680033862.01.T01;Parent=MsG0680033862.01;Name=MsG0680033862.01.T01;_AED=0.40;_eAED=0.40;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|171
Chr6	exon	75127112	75127627	75127112	ID=MsG0680033862.01.T01:exon:7633;Parent=MsG0680033862.01.T01
Chr6	CDS	75127112	75127627	75127112	ID=MsG0680033862.01.T01:cds;Parent=MsG0680033862.01.T01
Chr6	mRNA	75127424	75127627	75127424	ID=MsG0680033862.01.T02;Parent=MsG0680033862.01;Name=MsG0680033862.01.T02;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|67
Chr6	exon	75127424	75127627	75127424	ID=MsG0680033862.01.T02:exon:7634;Parent=MsG0680033862.01.T02
Chr6	CDS	75127424	75127627	75127424	ID=MsG0680033862.01.T02:cds;Parent=MsG0680033862.01.T02

Gene Sequence

>MsG0680033862.01.T01

ATGGCAGCTTTCTTAGATGAGTTGGTTGAGGATCCTCGTATTGAAGTGGGTACTCATGATTGGAATTCTATAATCCACGCTTTTTGTAAGGCTGGGAGGCTAGAAGATGCAAGAAGGACTTTCAGAAGGATGACATTCCTGCAATTCGAGCCGAATGACCAGACATACTTGTCCCTGATTAACGGATATGTTTCAGCAGAAAAATATTTTGATGCAATGATGTTGTGGAATGAGGTTAAGAGAAAGTTAGCAGGGGATGGGCCGAAGGGGATCAAATTTGACCAGAATCTGATTGATGCATTCCTGTATGCTATGGTCAAGGGAGGTTTCTTTGATGCTGTGATGCAAGTTGTGGAGAAATCTAAAGAGATGAAAATATTTGTTGATAAGTGGAGATACAAGCAAGCATTCATGGAGACACATAAAAAGCTCAAAGTGGCCAGGTTAAGGAAAAAGAATGTCAGAAAAATGGAAGCGCTTATTGCTTTTAAGAATTGGGCTGGCTTAAATGCATGA

>MsG0680033862.01.T02

ATGGTCAAGGGAGGTTTCTTTGATGCTGTGATGCAAGTTGTGGAGAAATCTAAAGAGATGAAAATATTTGTTGATAAGTGGAGATACAAGCAAGCATTCATGGAGACACATAAAAAGCTCAAAGTGGCCAGGTTAAGGAAAAAGAATGTCAGAAAAATGGAAGCGCTTATTGCTTTTAAGAATTGGGCTGGCTTAAATGCATGA

Protein sequence

>MsG0680033862.01.T01

MAAFLDELVEDPRIEVGTHDWNSIIHAFCKAGRLEDARRTFRRMTFLQFEPNDQTYLSLINGYVSAEKYFDAMMLWNEVKRKLAGDGPKGIKFDQNLIDAFLYAMVKGGFFDAVMQVVEKSKEMKIFVDKWRYKQAFMETHKKLKVARLRKKNVRKMEALIAFKNWAGLNA*

>MsG0680033862.01.T02

MVKGGFFDAVMQVVEKSKEMKIFVDKWRYKQAFMETHKKLKVARLRKKNVRKMEALIAFKNWAGLNA*