AlfalfaGEDB | MsG0280011217.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011217.01

KEGG: -
GO: GO:0005515
PFAM: PF01535

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011217.01.T01	XP_024631394.1	48.352	364	108	5	51	337	14	374	8.51E-89	290
MsG0280011217.01.T01	XP_024631394.1	56.458	271	39	5	135	345	236	487	1.33E-80	268

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011217.01.T01	P0C8R0	43.103	232	72	4	176	347	277	508	7.47E-49	177

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011217.01.T01	A2Q460	48.352	364	108	5	51	337	14	374	4.06e-89	290

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047957.01	MsG0280011217.01	0.821156	4.690144e-53	3.785986e-50
MsG0080047959.01	MsG0280011217.01	0.808498	3.007791e-50	1.722489e-47
MsG0080048683.01	MsG0280011217.01	0.812822	3.494191e-51	2.244053e-48
MsG0080048856.01	MsG0280011217.01	0.819322	1.234069e-52	9.465247e-50
MsG0080048892.01	MsG0280011217.01	0.806147	9.473026e-50	5.103709e-47
MsG0180000065.01	MsG0280011217.01	0.801681	8.027431e-49	3.857081e-46
MsG0180000091.01	MsG0280011217.01	0.819986	8.706763e-53	6.803627e-50
MsG0180000145.01	MsG0280011217.01	0.823912	1.072758e-53	9.361761e-51
MsG0180000470.01	MsG0280011217.01	0.832215	1.073573e-55	1.191972e-52
MsG0180000649.01	MsG0280011217.01	0.802332	5.900386e-49	2.882596e-46

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011217.01.T01	MTR_2g100200	48.352	364	108	5	51	337	14	374	1.03e-92	290
MsG0280011217.01.T01	MTR_2g100200	56.458	271	39	5	135	345	236	487	1.61e-84	268
MsG0280011217.01.T01	MTR_7g066990	27.723	202	107	6	157	336	209	393	1.15e-12	70.5
MsG0280011217.01.T01	MTR_5g077220	26.953	256	154	8	106	338	359	604	2.51e-12	69.3
MsG0280011217.01.T01	MTR_8g105170	26.000	250	146	5	157	385	334	565	2.18e-11	66.6
MsG0280011217.01.T01	MTR_8g054410	25.100	251	137	8	100	329	332	552	2.23e-11	66.2
MsG0280011217.01.T01	MTR_6g072030	25.248	202	115	4	157	337	247	433	2.68e-11	65.9
MsG0280011217.01.T01	MTR_6g075180	22.619	252	136	5	157	358	245	487	6.08e-11	64.7
MsG0280011217.01.T01	MTR_3g114700	22.180	266	149	8	139	358	150	403	7.73e-11	64.3
MsG0280011217.01.T01	MTR_7g103600	28.261	184	104	5	176	342	125	297	9.74e-11	64.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011217.01.T01	AT5G43820	43.103	232	72	4	176	347	277	508	7.61e-50	177
MsG0280011217.01.T01	AT5G43820	29.201	363	156	8	68	338	41	394	4.46e-32	128
MsG0280011217.01.T01	AT5G41170	26.190	210	118	6	148	335	54	248	9.63e-12	67.0
MsG0280011217.01.T01	AT5G41170	26.442	208	116	6	149	334	155	347	1.04e-11	67.0
MsG0280011217.01.T01	AT5G41170	26.442	208	116	6	149	334	155	347	1.09e-11	67.0
MsG0280011217.01.T01	AT5G39710	24.658	219	127	5	141	337	351	553	3.92e-11	65.5

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Find 86 sgRNAs with CRISPR-Local

Find 336 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGCTGTTCTTGTCATGG+AGG	0.741667	2:-81063084	MsG0280011217.01.T01:CDS
AAATGGTGAAATTGAATGAG+TGG	0.645651	2:-81066222	MsG0280011217.01.T01:CDS
AACATTGTTAAGTGTATGGC+AGG	0.509537	2:-81063189	MsG0280011217.01.T01:intron
AAGGAGGTTATAGTCCCAGT+AGG	0.631328	2:+81063348	None:intergenic
AAGGAGTTGTGCCTCCTACT+GGG	0.549624	2:-81063362	MsG0280011217.01.T01:CDS
AAGTGCATCGGCCACTTTCC+TGG	0.348306	2:+81063410	None:intergenic
AATTCAGTTGTTTGGGAATT+TGG	0.146474	2:-81063896	MsG0280011217.01.T01:CDS
AATTGAATGAGTGGCGAAAG+AGG	0.598233	2:-81066213	MsG0280011217.01.T01:CDS
ACCACGTAGTTTATCTTCAG+GGG	0.614563	2:+81064256	None:intergenic
AGTATTGAACTTTGGGAATT+TGG	0.122868	2:-81064144	MsG0280011217.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATACGTTACCAATTTCGTA+TGG	+	Chr2:81064802-81064821	None:intergenic	30.0%
AATTCAGTTGTTTGGGAATT+TGG	-	Chr2:81063524-81063543	MsG0280011217.01.T01:CDS	30.0%
ACGAAAATGACCTATATTAC+AGG	-	Chr2:81061581-81061600	MsG0280011217.01.T01:intron	30.0%
ATCACAGAAACTAGAAGAAT+TGG	+	Chr2:81062924-81062943	None:intergenic	30.0%
ATCTATTATCTGCATATGCA+TGG	-	Chr2:81065497-81065516	MsG0280011217.01.T01:intron	30.0%
ATGAGAAACGTTGATGTAAT+TGG	+	Chr2:81063018-81063037	None:intergenic	30.0%
ATGAGATTGAGAAGTTATGA+AGG	-	Chr2:81063722-81063741	MsG0280011217.01.T01:intron	30.0%
CAAAAGCAATTCAGTTGTTT+GGG	-	Chr2:81063517-81063536	MsG0280011217.01.T01:intron	30.0%
CCAAATCGTAAATCTAGAAA+AGG	+	Chr2:81064684-81064703	None:intergenic	30.0%
CCAGATATGTGAAAATCAAA+TGG	+	Chr2:81064571-81064590	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	81061200	81066242	81061200	ID=MsG0280011217.01;Name=MsG0280011217.01
Chr2	mRNA	81061200	81066242	81061200	ID=MsG0280011217.01.T01;Parent=MsG0280011217.01;Name=MsG0280011217.01.T01;_AED=0.56;_eAED=0.57;_QI=0\|0\|0\|0.71\|1\|1\|7\|0\|433
Chr2	exon	81066154	81066242	81066154	ID=MsG0280011217.01.T01:exon:11989;Parent=MsG0280011217.01.T01
Chr2	exon	81064130	81064508	81064130	ID=MsG0280011217.01.T01:exon:11988;Parent=MsG0280011217.01.T01
Chr2	exon	81063886	81064016	81063886	ID=MsG0280011217.01.T01:exon:11987;Parent=MsG0280011217.01.T01
Chr2	exon	81063520	81063630	81063520	ID=MsG0280011217.01.T01:exon:11986;Parent=MsG0280011217.01.T01
Chr2	exon	81063276	81063434	81063276	ID=MsG0280011217.01.T01:exon:11985;Parent=MsG0280011217.01.T01
Chr2	exon	81063049	81063199	81063049	ID=MsG0280011217.01.T01:exon:11984;Parent=MsG0280011217.01.T01
Chr2	exon	81061200	81061481	81061200	ID=MsG0280011217.01.T01:exon:11983;Parent=MsG0280011217.01.T01
Chr2	CDS	81066154	81066242	81066154	ID=MsG0280011217.01.T01:cds;Parent=MsG0280011217.01.T01
Chr2	CDS	81064130	81064508	81064130	ID=MsG0280011217.01.T01:cds;Parent=MsG0280011217.01.T01
Chr2	CDS	81063886	81064016	81063886	ID=MsG0280011217.01.T01:cds;Parent=MsG0280011217.01.T01
Chr2	CDS	81063520	81063630	81063520	ID=MsG0280011217.01.T01:cds;Parent=MsG0280011217.01.T01
Chr2	CDS	81063276	81063434	81063276	ID=MsG0280011217.01.T01:cds;Parent=MsG0280011217.01.T01
Chr2	CDS	81063049	81063199	81063049	ID=MsG0280011217.01.T01:cds;Parent=MsG0280011217.01.T01
Chr2	CDS	81061200	81061481	81061200	ID=MsG0280011217.01.T01:cds;Parent=MsG0280011217.01.T01

Gene Sequence

>MsG0280011217.01.T01

ATGGTGAAATTGAATGAGTGGCGAAAGAGGGTGAAAATGGTGAAGTGCAAGATCGATGGAGAAGAAGAGGATGAAAGAGAAAGTGTAGATTTCTGTGATTATTACATTCTTCATGGCGTTTCGATTTTTGCAATTCGTGCTTCACTTCTCAAAACCAAACACCCTCTTCCCCAATTACATCAACGTTTCTCATCGCTACAAAATTCTTCAAACCTTGATGAACGCCTCATTCTCCATCAAATCTCTCAACTTTTACCAATACCCACTTCCAAAACCTCAGAATCACAATCAGAATCCAAATCCGTTGATGGGTTTTTGTCCCCTGAAGATAAACTACGTGGTATCTTTCTTCAAAAACTAAAGGGTAAGGCTGCAATTGAACAAGCTTTATCCAATGTGTGTATTGATGTAAATGTTGATATTATTGGTAAAGTATTGAACTTTGGGAATTTGGGTGGTGAAGCAATGGTTTTAGATGAGATGAGATTGAATGGTGTGAAAGCTGATTTGTTAATGTTATCTATTGTTATTGATAGTTTTGTCAATGCCGGTCATGTGTCAAAAGCAATTCAGTTGTTTGGGAATTTGGATGATCTCGGAGCAGGTAGAATGAATGAGGCTGTTGAGGTTTTCGGTAGTATGAAGGAGAAAGACACTGCTATCTATAATGCAATGATTTTTAATTTCATTTCGATTGGAGATTTTGATGGGACCAGGAAAGTGGCCGATGCACTTTTGATGTTTGATGAGATGTTAAGGCAAGGAGTTGTGCCTCCTACTGGGACTATAACCTCCTTCATCAAACAGTTATGCAGCTATGGTCCACCATATGCAGCTATGATGATCTACAAGAAGACAAGAAAATTGGATGTATGGCAGGAGATGCAAGAATGTGGGTATAGTTCAGATGTTGAAGTTTACGAGTACATCATAAGTGGCCTTTACAACATAGGACAACTTGAAAATGCTGTTCTTGTCATGGAGGAGGCTTTGCGCAAAGGGTTCTGTCCCAGCAGGCTTGATTCCAACAAGATGGATACCATGCAAGCTTCCATAAGCAAGATTTCTGACATGATCTCAAATCTTCAGCACAAAAACATTGAAACCGTAACAGATCTTGGACCAGCAGTCAAGAAGAACGACACACTTACAAACTCGGATCGAGATTCAGATGATGATCCAGACCCGTTCTTGGATGAAAAAGCTGGTTTTACACCTACCTTCAAAGGCACGGATCCAATAGGGTTGCTGGCTCTCACTGACAAATTATCGCTGTGCAGAAAGTCGAACCTTCAAAGCTGA

Protein sequence

>MsG0280011217.01.T01

MVKLNEWRKRVKMVKCKIDGEEEDERESVDFCDYYILHGVSIFAIRASLLKTKHPLPQLHQRFSSLQNSSNLDERLILHQISQLLPIPTSKTSESQSESKSVDGFLSPEDKLRGIFLQKLKGKAAIEQALSNVCIDVNVDIIGKVLNFGNLGGEAMVLDEMRLNGVKADLLMLSIVIDSFVNAGHVSKAIQLFGNLDDLGAGRMNEAVEVFGSMKEKDTAIYNAMIFNFISIGDFDGTRKVADALLMFDEMLRQGVVPPTGTITSFIKQLCSYGPPYAAMMIYKKTRKLDVWQEMQECGYSSDVEVYEYIISGLYNIGQLENAVLVMEEALRKGFCPSRLDSNKMDTMQASISKISDMISNLQHKNIETVTDLGPAVKKNDTLTNSDRDSDDDPDPFLDEKAGFTPTFKGTDPIGLLALTDKLSLCRKSNLQS*