AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010796.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010796.01.T01 MTR_2g090885 96.522 115 4 0 1 115 240 354 5.33e-78 234
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010796.01.T01 AT1G06270 63.478 115 42 0 1 115 229 343 2.63e-49 160

Find 29 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGCCTCACTTTGATATA+TGG 0.223784 2:-75189500 None:intergenic
TTCACCATTGTTCCATGATC+TGG 0.271071 2:-75189290 None:intergenic
GGAGAGACGCGAGTATGTTT+TGG 0.327273 2:+75189436 MsG0280010796.01.T01:CDS
AAGCAAATGGTGGCGAAATA+CGG 0.373399 2:+75189260 MsG0280010796.01.T01:CDS
TCACCATTGTTCCATGATCT+GGG 0.374962 2:-75189289 None:intergenic
TACGGGCAAACAAAGAGATA+TGG 0.420506 2:+75189327 MsG0280010796.01.T01:CDS
GATTGAGTTCCTTGAGATAG+AGG 0.423715 2:+75189364 MsG0280010796.01.T01:CDS
TTGGCTGGAAAGGTTGCTAT+GGG 0.425539 2:+75189455 MsG0280010796.01.T01:CDS
AGTTATGAGTTGGTGATTGA+AGG 0.487641 2:+75189407 MsG0280010796.01.T01:CDS
CCAAGATTTAATGAAGCAAA+TGG 0.503573 2:+75189247 None:intergenic
AGCAAATGGTGGCGAAATAC+GGG 0.506036 2:+75189261 MsG0280010796.01.T01:CDS
TTTGGCTGGAAAGGTTGCTA+TGG 0.507338 2:+75189454 MsG0280010796.01.T01:CDS
GTTGGTGATTGAAGGTTGCT+TGG 0.507626 2:+75189415 MsG0280010796.01.T01:CDS
CACCATTGTTCCATGATCTG+GGG 0.508647 2:-75189288 None:intergenic
TACGGGTTGACCCCAGATCA+TGG 0.517436 2:+75189278 MsG0280010796.01.T01:CDS
GTGAGGCAGAAGATAATTGA+AGG 0.519423 2:+75189512 MsG0280010796.01.T01:CDS
GCAGAAGATAATTGAAGGGT+TGG 0.534726 2:+75189517 MsG0280010796.01.T01:CDS
GCTATGGGGATGACAGAAAG+AGG 0.555551 2:+75189470 MsG0280010796.01.T01:CDS
TATCTTCCACTCATCAATGC+TGG 0.559286 2:-75189540 None:intergenic
GAGATAGAGGGAAACTCTGT+CGG 0.569139 2:+75189377 MsG0280010796.01.T01:CDS
ATTGAGTTCCTTGAGATAGA+GGG 0.582699 2:+75189365 MsG0280010796.01.T01:CDS
CGGATTTGAGAGTTATGAGT+TGG 0.595180 2:+75189397 MsG0280010796.01.T01:CDS
CAGAGTTTCCCTCTATCTCA+AGG 0.605707 2:-75189373 None:intergenic
TGAGGCAGAAGATAATTGAA+GGG 0.627213 2:+75189513 MsG0280010796.01.T01:CDS
TGGCTGGAAAGGTTGCTATG+GGG 0.628225 2:+75189456 MsG0280010796.01.T01:CDS
GACCCCAGATCATGGAACAA+TGG 0.637060 2:+75189286 MsG0280010796.01.T01:CDS
TTATTCCATATATCAAAGTG+AGG 0.652765 2:+75189495 MsG0280010796.01.T01:CDS
AGATTTAATGAAGCAAATGG+TGG 0.664608 2:+75189250 None:intergenic
GGTTGGCCAGCATTGATGAG+TGG 0.665276 2:+75189534 MsG0280010796.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTATTCCATATATCAAAGTG+AGG + Chr2:75189495-75189514 MsG0280010796.01.T01:CDS 25.0%
!! TGAAAATTTTGTCAGCATTA+CGG + Chr2:75189309-75189328 MsG0280010796.01.T01:CDS 25.0%
! GAAAATTTTGTCAGCATTAC+GGG + Chr2:75189310-75189329 MsG0280010796.01.T01:CDS 30.0%
ATTGAGTTCCTTGAGATAGA+GGG + Chr2:75189365-75189384 MsG0280010796.01.T01:CDS 35.0%
TGAGGCAGAAGATAATTGAA+GGG + Chr2:75189513-75189532 MsG0280010796.01.T01:CDS 35.0%
TTCTGCCTCACTTTGATATA+TGG - Chr2:75189503-75189522 None:intergenic 35.0%
!! AGTTATGAGTTGGTGATTGA+AGG + Chr2:75189407-75189426 MsG0280010796.01.T01:CDS 35.0%
CGGATTTGAGAGTTATGAGT+TGG + Chr2:75189397-75189416 MsG0280010796.01.T01:CDS 40.0%
GATTGAGTTCCTTGAGATAG+AGG + Chr2:75189364-75189383 MsG0280010796.01.T01:CDS 40.0%
GCAGAAGATAATTGAAGGGT+TGG + Chr2:75189517-75189536 MsG0280010796.01.T01:CDS 40.0%
GTGAGGCAGAAGATAATTGA+AGG + Chr2:75189512-75189531 MsG0280010796.01.T01:CDS 40.0%
TACGGGCAAACAAAGAGATA+TGG + Chr2:75189327-75189346 MsG0280010796.01.T01:CDS 40.0%
TATCTTCCACTCATCAATGC+TGG - Chr2:75189543-75189562 None:intergenic 40.0%
TCACCATTGTTCCATGATCT+GGG - Chr2:75189292-75189311 None:intergenic 40.0%
TTCACCATTGTTCCATGATC+TGG - Chr2:75189293-75189312 None:intergenic 40.0%
! AAGCAAATGGTGGCGAAATA+CGG + Chr2:75189260-75189279 MsG0280010796.01.T01:CDS 40.0%
CACCATTGTTCCATGATCTG+GGG - Chr2:75189291-75189310 None:intergenic 45.0%
CAGAGTTTCCCTCTATCTCA+AGG - Chr2:75189376-75189395 None:intergenic 45.0%
GAGATAGAGGGAAACTCTGT+CGG + Chr2:75189377-75189396 MsG0280010796.01.T01:CDS 45.0%
TTGGCTGGAAAGGTTGCTAT+GGG + Chr2:75189455-75189474 MsG0280010796.01.T01:CDS 45.0%
TTTGGCTGGAAAGGTTGCTA+TGG + Chr2:75189454-75189473 MsG0280010796.01.T01:CDS 45.0%
! AGCAAATGGTGGCGAAATAC+GGG + Chr2:75189261-75189280 MsG0280010796.01.T01:CDS 45.0%
!! GTTGGTGATTGAAGGTTGCT+TGG + Chr2:75189415-75189434 MsG0280010796.01.T01:CDS 45.0%
!!! CGAGTATGTTTTGGCTGGAA+AGG + Chr2:75189445-75189464 MsG0280010796.01.T01:CDS 45.0%
GACCCCAGATCATGGAACAA+TGG + Chr2:75189286-75189305 MsG0280010796.01.T01:CDS 50.0%
GCTATGGGGATGACAGAAAG+AGG + Chr2:75189470-75189489 MsG0280010796.01.T01:CDS 50.0%
TGGCTGGAAAGGTTGCTATG+GGG + Chr2:75189456-75189475 MsG0280010796.01.T01:CDS 50.0%
! GGAGAGACGCGAGTATGTTT+TGG + Chr2:75189436-75189455 MsG0280010796.01.T01:CDS 50.0%
!!! AGACGCGAGTATGTTTTGGC+TGG + Chr2:75189440-75189459 MsG0280010796.01.T01:CDS 50.0%
! TACGGGTTGACCCCAGATCA+TGG + Chr2:75189278-75189297 MsG0280010796.01.T01:CDS 55.0%
!! GGTTGGCCAGCATTGATGAG+TGG + Chr2:75189534-75189553 MsG0280010796.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 75189257 75189604 75189257 ID=MsG0280010796.01;Name=MsG0280010796.01
Chr2 mRNA 75189257 75189604 75189257 ID=MsG0280010796.01.T01;Parent=MsG0280010796.01;Name=MsG0280010796.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|115
Chr2 exon 75189257 75189604 75189257 ID=MsG0280010796.01.T01:exon:19959;Parent=MsG0280010796.01.T01
Chr2 CDS 75189257 75189604 75189257 ID=MsG0280010796.01.T01:cds;Parent=MsG0280010796.01.T01
Gene Sequence

>MsG0280010796.01.T01

ATGAAGCAAATGGTGGCGAAATACGGGTTGACCCCAGATCATGGAACAATGGTGAAAATTTTGTCAGCATTACGGGCAAACAAAGAGATATGGAAAGCTGTTGAGATGATTGAGTTCCTTGAGATAGAGGGAAACTCTGTCGGATTTGAGAGTTATGAGTTGGTGATTGAAGGTTGCTTGGAGAGACGCGAGTATGTTTTGGCTGGAAAGGTTGCTATGGGGATGACAGAAAGAGGTTTTATTCCATATATCAAAGTGAGGCAGAAGATAATTGAAGGGTTGGCCAGCATTGATGAGTGGAAGATAGCTTGTGCTGTGAGACAAAGATTCGCAACACTTAAATCTTAG

Protein sequence

>MsG0280010796.01.T01

MKQMVAKYGLTPDHGTMVKILSALRANKEIWKAVEMIEFLEIEGNSVGFESYELVIEGCLERREYVLAGKVAMGMTERGFIPYIKVRQKIIEGLASIDEWKIACAVRQRFATLKS*