AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036965.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036965.01.T01 MTR_7g024560 95.489 133 6 0 3 135 175 307 1.93e-92 270
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036965.01.T01 AT3G04020 50.382 131 62 2 4 134 112 239 1.85e-36 125
MsG0780036965.01.T01 AT3G04020 50.382 131 62 2 4 134 109 236 2.23e-36 125
MsG0780036965.01.T01 AT3G04020 50.382 131 62 2 4 134 177 304 5.92e-36 125

Find 39 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAAGAATCTGCTAATATT+TGG 0.219834 7:+17236535 MsG0780036965.01.T01:three_prime_UTR
ATTTATTATAACAAAATAAA+AGG 0.224914 7:-17236412 None:intergenic
TCAATCAACTTCGCAGAAAA+TGG 0.267617 7:-17235620 None:intergenic
TTCACCTAATTGATCAGCTT+TGG 0.322940 7:-17235517 None:intergenic
AGATCTTGCACATTTATTGA+TGG 0.337055 7:+17235450 MsG0780036965.01.T01:CDS
ACATCCAAAGCTGATCAATT+AGG 0.353849 7:+17235513 MsG0780036965.01.T01:CDS
CCAACGCCATCCTTTGAAAC+AGG 0.397576 7:-17236235 None:intergenic
GATGTGCTAGCAAAAGTTAC+TGG 0.434201 7:+17236211 MsG0780036965.01.T01:CDS
CTAAAACATGCAACCTTGGT+TGG 0.437153 7:+17236387 MsG0780036965.01.T01:exon
CTGTCAACATCTAGTTCAAA+TGG 0.447013 7:+17236321 MsG0780036965.01.T01:CDS
GATGTGGATGTTGAAGATTT+AGG 0.465087 7:+17235489 MsG0780036965.01.T01:CDS
ATCGAATCCCTTGCGATCAA+CGG 0.485133 7:-17236191 None:intergenic
TGGAAGAGGAGGTCATCAAA+AGG 0.490710 7:+17236341 MsG0780036965.01.T01:CDS
TAAACTAAAACATGCAACCT+TGG 0.497628 7:+17236383 MsG0780036965.01.T01:exon
AGTTACTGGTCCTGTTTCAA+AGG 0.504240 7:+17236225 MsG0780036965.01.T01:CDS
TCATTAAGTTACCTTTGCAT+TGG 0.518961 7:-17235708 None:intergenic
ACTGGTCCTGTTTCAAAGGA+TGG 0.521951 7:+17236229 MsG0780036965.01.T01:CDS
AATACCAATGGACAGAGCTC+AGG 0.523287 7:+17236260 MsG0780036965.01.T01:CDS
GGAAGAGGAGGTCATCAAAA+GGG 0.526294 7:+17236342 MsG0780036965.01.T01:CDS
TTTCTGCGAAGTTGATTGAT+GGG 0.526838 7:+17235624 MsG0780036965.01.T01:CDS
TGCACATTTATTGATGGCCA+TGG 0.529543 7:+17235456 MsG0780036965.01.T01:CDS
TGTAACATCCGTTGATCGCA+AGG 0.531235 7:+17236183 MsG0780036965.01.T01:CDS
CTCTACGCCTAGCTTCACTT+TGG 0.537449 7:-17235652 None:intergenic
TGAACCTGAGCTCTGTCCAT+TGG 0.545424 7:-17236264 None:intergenic
CAGGTTCATGTTCGAGCAAG+AGG 0.547326 7:+17236279 MsG0780036965.01.T01:CDS
ACATCTAGTTCAAATGGAAG+AGG 0.555304 7:+17236327 MsG0780036965.01.T01:CDS
CCTGTTTCAAAGGATGGCGT+TGG 0.557484 7:+17236235 MsG0780036965.01.T01:CDS
AAAAGGGTTTCAGCCTCTAG+TGG 0.558190 7:+17236358 MsG0780036965.01.T01:CDS
GGATTAACCAAAGTGAAGCT+AGG 0.579660 7:+17235645 MsG0780036965.01.T01:CDS
TTGACAGAATGTTTCAACAT+CGG 0.583310 7:-17236305 None:intergenic
ATGGCGTTGGTCAATACCAA+TGG 0.594282 7:+17236248 MsG0780036965.01.T01:CDS
GTAACATCCGTTGATCGCAA+GGG 0.604514 7:+17236184 MsG0780036965.01.T01:CDS
TCTAGTTCAAATGGAAGAGG+AGG 0.611304 7:+17236330 MsG0780036965.01.T01:CDS
TTACATGAACACCAATGCAA+AGG 0.620843 7:+17235697 MsG0780036965.01.T01:CDS
AAACAAAACATAAAAGCACA+AGG 0.660653 7:-17235676 None:intergenic
AAAATAAAAGGATCCAACCA+AGG 0.671507 7:-17236400 None:intergenic
ACATTTATTGATGGCCATGG+TGG 0.680039 7:+17235459 MsG0780036965.01.T01:CDS
AACAAAACATAAAAGCACAA+GGG 0.697914 7:-17235675 None:intergenic
CCACATCAAAAGTACCACCA+TGG 0.733695 7:-17235473 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAATGAACTGATATA+TGG - Chr7:17236094-17236113 None:intergenic 15.0%
!!! TCATATGACTAATATCTTTT+AGG - Chr7:17236448-17236467 None:intergenic 20.0%
! ATAGATTGTTTATCTTCGTA+TGG + Chr7:17235902-17235921 MsG0780036965.01.T01:intron 25.0%
! CAAAAGAATCTGCTAATATT+TGG + Chr7:17236535-17236554 MsG0780036965.01.T01:three_prime_UTR 25.0%
! CATTGATCATTCTTAGATAA+TGG + Chr7:17235808-17235827 MsG0780036965.01.T01:intron 25.0%
! TAGAGATGAATACTGATATA+TGG + Chr7:17236039-17236058 MsG0780036965.01.T01:intron 25.0%
!! AAACAAAACATAAAAGCACA+AGG - Chr7:17235679-17235698 None:intergenic 25.0%
!! AACAAAACATAAAAGCACAA+GGG - Chr7:17235678-17235697 None:intergenic 25.0%
!!! TGGTTTTAAATGCATTAGTT+CGG + Chr7:17235544-17235563 MsG0780036965.01.T01:intron 25.0%
AAAATAAAAGGATCCAACCA+AGG - Chr7:17236403-17236422 None:intergenic 30.0%
AGATCTTGCACATTTATTGA+TGG + Chr7:17235450-17235469 MsG0780036965.01.T01:CDS 30.0%
ATGAATACTGATATATGGCA+GGG + Chr7:17236044-17236063 MsG0780036965.01.T01:intron 30.0%
TAAACTAAAACATGCAACCT+TGG + Chr7:17236383-17236402 MsG0780036965.01.T01:exon 30.0%
TCATTAAGTTACCTTTGCAT+TGG - Chr7:17235711-17235730 None:intergenic 30.0%
TTGACAGAATGTTTCAACAT+CGG - Chr7:17236308-17236327 None:intergenic 30.0%
! GAACTAATGCATTTAAAACC+AGG - Chr7:17235545-17235564 None:intergenic 30.0%
!! AGGGTAAATTGTTTTGTCTT+AGG + Chr7:17236063-17236082 MsG0780036965.01.T01:intron 30.0%
!! ATTTTCCTCTTTTTTCGTCA+AGG + Chr7:17236501-17236520 MsG0780036965.01.T01:three_prime_UTR 30.0%
AAGCTACCATAATCTAGAGA+AGG - Chr7:17235987-17236006 None:intergenic 35.0%
ACATCCAAAGCTGATCAATT+AGG + Chr7:17235513-17235532 MsG0780036965.01.T01:CDS 35.0%
ATTCTCCTTGACGAAAAAAG+AGG - Chr7:17236509-17236528 None:intergenic 35.0%
GATGAATACTGATATATGGC+AGG + Chr7:17236043-17236062 MsG0780036965.01.T01:intron 35.0%
GATGTGGATGTTGAAGATTT+AGG + Chr7:17235489-17235508 MsG0780036965.01.T01:CDS 35.0%
TACCATAATCTAGAGAAGGA+TGG - Chr7:17235983-17236002 None:intergenic 35.0%
TCAATCAACTTCGCAGAAAA+TGG - Chr7:17235623-17235642 None:intergenic 35.0%
TGTCTTCTTGATTACAAGTG+TGG + Chr7:17235775-17235794 MsG0780036965.01.T01:intron 35.0%
TTAACAACTAACGAAGCATG+TGG + Chr7:17236140-17236159 MsG0780036965.01.T01:intron 35.0%
TTACATGAACACCAATGCAA+AGG + Chr7:17235697-17235716 MsG0780036965.01.T01:CDS 35.0%
TTCACCTAATTGATCAGCTT+TGG - Chr7:17235520-17235539 None:intergenic 35.0%
TTGTTTATCTTCGTATGGCA+TGG + Chr7:17235907-17235926 MsG0780036965.01.T01:intron 35.0%
! ACATCTAGTTCAAATGGAAG+AGG + Chr7:17236327-17236346 MsG0780036965.01.T01:CDS 35.0%
! CTGTCAACATCTAGTTCAAA+TGG + Chr7:17236321-17236340 MsG0780036965.01.T01:CDS 35.0%
! GTATTCATCTCTAAGAAGCA+TGG - Chr7:17236032-17236051 None:intergenic 35.0%
! GTTGCATAGCATTTTAGATG+CGG + Chr7:17235834-17235853 MsG0780036965.01.T01:intron 35.0%
! TTGCATAGCATTTTAGATGC+GGG + Chr7:17235835-17235854 MsG0780036965.01.T01:intron 35.0%
! TTTCTGCGAAGTTGATTGAT+GGG + Chr7:17235624-17235643 MsG0780036965.01.T01:CDS 35.0%
!! AAGCATGTGGTGTTGTATTA+AGG + Chr7:17236153-17236172 MsG0780036965.01.T01:intron 35.0%
!! TTTTCTGCGAAGTTGATTGA+TGG + Chr7:17235623-17235642 MsG0780036965.01.T01:CDS 35.0%
!!! GTTTTAGTTTAGTCCACTAG+AGG - Chr7:17236374-17236393 None:intergenic 35.0%
AAGTAACCAATTGCAGGATC+AGG - Chr7:17235753-17235772 None:intergenic 40.0%
AGTAACCAATTGCAGGATCA+GGG - Chr7:17235752-17235771 None:intergenic 40.0%
AGTTACTGGTCCTGTTTCAA+AGG + Chr7:17236225-17236244 MsG0780036965.01.T01:CDS 40.0%
CTAAAACATGCAACCTTGGT+TGG + Chr7:17236387-17236406 MsG0780036965.01.T01:exon 40.0%
GACATCAAGTAACCAATTGC+AGG - Chr7:17235759-17235778 None:intergenic 40.0%
GATGTGCTAGCAAAAGTTAC+TGG + Chr7:17236211-17236230 MsG0780036965.01.T01:CDS 40.0%
GGATTAACCAAAGTGAAGCT+AGG + Chr7:17235645-17235664 MsG0780036965.01.T01:CDS 40.0%
GTCCATCCTTCTCTAGATTA+TGG + Chr7:17235978-17235997 MsG0780036965.01.T01:intron 40.0%
TGATCAATTAGGTGAAGACC+TGG + Chr7:17235524-17235543 MsG0780036965.01.T01:intron 40.0%
! ACATTTATTGATGGCCATGG+TGG + Chr7:17235459-17235478 MsG0780036965.01.T01:CDS 40.0%
! TCTAGTTCAAATGGAAGAGG+AGG + Chr7:17236330-17236349 MsG0780036965.01.T01:CDS 40.0%
! TGCACATTTATTGATGGCCA+TGG + Chr7:17235456-17235475 MsG0780036965.01.T01:CDS 40.0%
AAAAGGGTTTCAGCCTCTAG+TGG + Chr7:17236358-17236377 MsG0780036965.01.T01:CDS 45.0%
AATACCAATGGACAGAGCTC+AGG + Chr7:17236260-17236279 MsG0780036965.01.T01:CDS 45.0%
ACTGGTCCTGTTTCAAAGGA+TGG + Chr7:17236229-17236248 MsG0780036965.01.T01:CDS 45.0%
ATCGAATCCCTTGCGATCAA+CGG - Chr7:17236194-17236213 None:intergenic 45.0%
GGAAGAGGAGGTCATCAAAA+GGG + Chr7:17236342-17236361 MsG0780036965.01.T01:CDS 45.0%
GGTAGCTTCTGTAGTATTGC+TGG + Chr7:17235999-17236018 MsG0780036965.01.T01:intron 45.0%
GTAACATCCGTTGATCGCAA+GGG + Chr7:17236184-17236203 MsG0780036965.01.T01:CDS 45.0%
TGGAAGAGGAGGTCATCAAA+AGG + Chr7:17236341-17236360 MsG0780036965.01.T01:CDS 45.0%
TGTAACATCCGTTGATCGCA+AGG + Chr7:17236183-17236202 MsG0780036965.01.T01:CDS 45.0%
TTACACCCTGATCCTGCAAT+TGG + Chr7:17235744-17235763 MsG0780036965.01.T01:intron 45.0%
! CCATGGTGGTACTTTTGATG+TGG + Chr7:17235473-17235492 MsG0780036965.01.T01:CDS 45.0%
!! ATGGCGTTGGTCAATACCAA+TGG + Chr7:17236248-17236267 MsG0780036965.01.T01:CDS 45.0%
!! CCACATCAAAAGTACCACCA+TGG - Chr7:17235476-17235495 None:intergenic 45.0%
!! ATTTATTATAACAAAATAAA+AGG - Chr7:17236415-17236434 None:intergenic 5.0%
CAGGTTCATGTTCGAGCAAG+AGG + Chr7:17236279-17236298 MsG0780036965.01.T01:CDS 50.0%
CCTGTTTCAAAGGATGGCGT+TGG + Chr7:17236235-17236254 MsG0780036965.01.T01:CDS 50.0%
CTCTACGCCTAGCTTCACTT+TGG - Chr7:17235655-17235674 None:intergenic 50.0%
TGAACCTGAGCTCTGTCCAT+TGG - Chr7:17236267-17236286 None:intergenic 50.0%
!! CCAACGCCATCCTTTGAAAC+AGG - Chr7:17236238-17236257 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 17235444 17236609 17235444 ID=MsG0780036965.01;Name=MsG0780036965.01
Chr7 mRNA 17235444 17236609 17235444 ID=MsG0780036965.01.T01;Parent=MsG0780036965.01;Name=MsG0780036965.01.T01;_AED=0.49;_eAED=0.49;_QI=0|1|0.66|1|1|1|3|219|135
Chr7 exon 17235444 17235534 17235444 ID=MsG0780036965.01.T01:exon:11544;Parent=MsG0780036965.01.T01
Chr7 exon 17235618 17235718 17235618 ID=MsG0780036965.01.T01:exon:11545;Parent=MsG0780036965.01.T01
Chr7 exon 17236175 17236609 17236175 ID=MsG0780036965.01.T01:exon:11546;Parent=MsG0780036965.01.T01
Chr7 CDS 17235444 17235534 17235444 ID=MsG0780036965.01.T01:cds;Parent=MsG0780036965.01.T01
Chr7 CDS 17235618 17235718 17235618 ID=MsG0780036965.01.T01:cds;Parent=MsG0780036965.01.T01
Chr7 CDS 17236175 17236390 17236175 ID=MsG0780036965.01.T01:cds;Parent=MsG0780036965.01.T01
Chr7 three_prime_UTR 17236391 17236609 17236391 ID=MsG0780036965.01.T01:three_prime_utr;Parent=MsG0780036965.01.T01
Gene Sequence

>MsG0780036965.01.T01

ATGCTCAGATCTTGCACATTTATTGATGGCCATGGTGGTACTTTTGATGTGGATGTTGAAGATTTAGGAACATCCAAAGCTGATCAATTAGCTCCATTTTCTGCGAAGTTGATTGATGGGATTAACCAAAGTGAAGCTAGGCGTAGAGCCCTTGTGCTTTTATGTTTTGTTTACATGAACACCAATGCAAAGGATGCTTATGTAACATCCGTTGATCGCAAGGGATTCGATGTGCTAGCAAAAGTTACTGGTCCTGTTTCAAAGGATGGCGTTGGTCAATACCAATGGACAGAGCTCAGGTTCATGTTCGAGCAAGAGGCAACCGATGTTGAAACATTCTGTCAACATCTAGTTCAAATGGAAGAGGAGGTCATCAAAAGGGTTTCAGCCTCTAGTGGACTAAACTAA

Protein sequence

>MsG0780036965.01.T01

MLRSCTFIDGHGGTFDVDVEDLGTSKADQLAPFSAKLIDGINQSEARRRALVLLCFVYMNTNAKDAYVTSVDRKGFDVLAKVTGPVSKDGVGQYQWTELRFMFEQEATDVETFCQHLVQMEEEVIKRVSASSGLN*