AlfalfaGEDB | MsG0380015466.01

M. sativa cultivar ZhongmuNo.1 / MsG0380015466.01

KEGG: K01802
GO: GO:0000413 GO:0003755 GO:0006457
PFAM: PF00160

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015466.01.T01	PNX99573.1	93.373	166	7	1	1	166	1	162	8.81E-106	309

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015466.01.T01	Q9SP02	79.375	160	29	1	5	164	47	202	2.27E-91	267

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015466.01.T01	A0A2K3N947	93.373	166	7	1	1	166	1	162	4.21e-106	309

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047791.01	MsG0380015466.01	0.816037	6.798718e-52	4.765094e-49
MsG0080048817.01	MsG0380015466.01	0.831937	1.257138e-55	1.384696e-52
MsG0180000139.01	MsG0380015466.01	0.830204	3.350354e-55	3.506270e-52
MsG0180000152.01	MsG0380015466.01	0.841390	4.892277e-58	7.178000e-55
MsG0180000544.01	MsG0380015466.01	-0.803853	2.858428e-49	1.451891e-46
MsG0180000614.01	MsG0380015466.01	0.825807	3.832325e-54	3.530177e-51
MsG0180001118.01	MsG0380015466.01	0.809862	1.534273e-50	9.109804e-48
MsG0180001131.01	MsG0380015466.01	0.825503	4.523017e-54	4.130188e-51
MsG0180001141.01	MsG0380015466.01	0.827640	1.399505e-54	1.358916e-51
MsG0180001153.01	MsG0380015466.01	0.808468	3.051694e-50	1.746292e-47

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PPI

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Gene1	Gene2	Type
MsG0280006841.01	MsG0380015466.01	PPI
MsG0380015466.01	MsG0380018047.01	PPI
MsG0380015466.01	MsG0280006841.01	PPI
MsG0380015466.01	MsG0780041153.01	PPI
MsG0480021461.01	MsG0380015466.01	PPI
MsG0780041153.01	MsG0380015466.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015466.01.T01	MTR_3g070500	93.750	160	6	1	7	166	78	233	2.62e-105	301
MsG0380015466.01.T01	MTR_3g463900	81.132	159	26	1	6	164	47	201	5.81e-93	268
MsG0380015466.01.T01	MTR_7g116340	66.871	163	48	2	4	165	15	172	4.23e-75	222
MsG0380015466.01.T01	MTR_3g070500	92.174	115	5	1	7	121	78	188	2.22e-72	216
MsG0380015466.01.T01	MTR_4g075290	63.750	160	54	1	6	165	17	172	1.11e-71	214
MsG0380015466.01.T01	MTR_3g117750	65.409	159	51	1	7	165	26	180	1.04e-70	212
MsG0380015466.01.T01	MTR_4g073650	59.748	159	59	2	7	164	60	214	2.64e-62	192
MsG0380015466.01.T01	MTR_1g085560	60.494	162	51	3	5	164	97	247	7.22e-62	191
MsG0380015466.01.T01	MTR_7g095750	60.870	161	58	2	8	167	22	178	4.57e-61	199
MsG0380015466.01.T01	MTR_7g110980	59.748	159	52	2	7	164	110	257	4.16e-60	187

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015466.01.T01	AT5G58710	79.375	160	29	1	5	164	47	202	2.32e-92	267
MsG0380015466.01.T01	AT2G29960	80.000	160	28	1	5	164	44	199	2.49e-91	265
MsG0380015466.01.T01	AT2G29960	80.000	160	28	1	5	164	44	199	2.49e-91	265
MsG0380015466.01.T01	AT3G55920	74.375	160	37	1	6	165	72	227	9.33e-85	249
MsG0380015466.01.T01	AT2G29960	75.000	160	26	2	5	164	44	189	6.62e-82	240
MsG0380015466.01.T01	AT2G21130	66.460	161	50	1	6	166	18	174	2.08e-75	223
MsG0380015466.01.T01	AT3G56070	66.258	163	51	1	4	166	15	173	2.95e-74	220
MsG0380015466.01.T01	AT3G56070	66.258	163	51	1	4	166	15	173	2.95e-74	220
MsG0380015466.01.T01	AT4G38740	65.000	160	52	1	6	165	17	172	1.49e-73	218
MsG0380015466.01.T01	AT2G16600	65.432	162	50	2	5	165	17	173	1.87e-73	218

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Find 55 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAGTCTTGAAATTCAG+AGG	0.576402	3:+70578631	None:intergenic
AAAATGACGTTTCTTGCTTC+AGG	0.313364	3:-70579937	MsG0380015466.01.T01:CDS
AACTTCAAGCTGAAGCATAC+TGG	0.421126	3:-70579504	MsG0380015466.01.T01:CDS
AACTTCCGTGCACTTTGTAC+AGG	0.492063	3:-70579779	MsG0380015466.01.T01:intron
AAGCTGAAGCATACTGGTCC+AGG	0.505316	3:-70579498	MsG0380015466.01.T01:intron
AAGTAAATGAACAAAATTTG+CGG	0.458182	3:-70578801	MsG0380015466.01.T01:three_prime_UTR
AAGTGGCTCTCCCAAACGTA+CGG	0.616615	3:-70578900	MsG0380015466.01.T01:CDS
AATACCAAGCTTCATGGTTC+AGG	0.307705	3:-70579595	MsG0380015466.01.T01:CDS
AATATGACAACCGTACGTTT+GGG	0.483233	3:+70578890	None:intergenic
AATCACATTTCAGGAGAGAA+AGG	0.447009	3:-70579671	MsG0380015466.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACATATGGATGCATGTT+AGG	+	Chr3:70579510-70579529	None:intergenic	30.0%
ACTTATCAATGGCAAATTCT+GGG	-	Chr3:70579165-70579184	MsG0380015466.01.T01:intron	30.0%
ATTGAACAGAATCACATTTC+AGG	-	Chr3:70578838-70578857	MsG0380015466.01.T01:three_prime_UTR	30.0%
GGATCAATCTTGAAATTTCT+AGG	-	Chr3:70578800-70578819	MsG0380015466.01.T01:three_prime_UTR	30.0%
TAACAACAGTAAAAACTAGC+TGG	-	Chr3:70579216-70579235	MsG0380015466.01.T01:intron	30.0%
TACTTATCAATGGCAAATTC+TGG	-	Chr3:70579164-70579183	MsG0380015466.01.T01:intron	30.0%
TAGGTTGTGAGATTAAAGAA+AGG	+	Chr3:70579491-70579510	None:intergenic	30.0%
AATATGACAACCGTACGTTT+GGG	+	Chr3:70579631-70579650	None:intergenic	35.0%
AATCACATTTCAGGAGAGAA+AGG	-	Chr3:70578847-70578866	MsG0380015466.01.T01:exon	35.0%
AGAGGTGGAGAATCAATTTA+TGG	-	Chr3:70578972-70578991	MsG0380015466.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	70578584	70579956	70578584	ID=MsG0380015466.01;Name=MsG0380015466.01
Chr3	mRNA	70578584	70579956	70578584	ID=MsG0380015466.01.T01;Parent=MsG0380015466.01;Name=MsG0380015466.01.T01;_AED=0.29;_eAED=0.29;_QI=0\|0.75\|0.6\|1\|0.75\|0.8\|5\|278\|167
Chr3	exon	70578584	70579003	70578584	ID=MsG0380015466.01.T01:exon:462;Parent=MsG0380015466.01.T01
Chr3	exon	70579303	70579378	70579303	ID=MsG0380015466.01.T01:exon:461;Parent=MsG0380015466.01.T01
Chr3	exon	70579499	70579693	70579499	ID=MsG0380015466.01.T01:exon:460;Parent=MsG0380015466.01.T01
Chr3	exon	70579780	70579803	70579780	ID=MsG0380015466.01.T01:exon:459;Parent=MsG0380015466.01.T01
Chr3	exon	70579890	70579956	70579890	ID=MsG0380015466.01.T01:exon:458;Parent=MsG0380015466.01.T01
Chr3	CDS	70579890	70579956	70579890	ID=MsG0380015466.01.T01:cds;Parent=MsG0380015466.01.T01
Chr3	CDS	70579780	70579803	70579780	ID=MsG0380015466.01.T01:cds;Parent=MsG0380015466.01.T01
Chr3	CDS	70579499	70579693	70579499	ID=MsG0380015466.01.T01:cds;Parent=MsG0380015466.01.T01
Chr3	CDS	70579303	70579378	70579303	ID=MsG0380015466.01.T01:cds;Parent=MsG0380015466.01.T01
Chr3	CDS	70578862	70579003	70578862	ID=MsG0380015466.01.T01:cds;Parent=MsG0380015466.01.T01
Chr3	three_prime_UTR	70578584	70578861	70578584	ID=MsG0380015466.01.T01:three_prime_utr;Parent=MsG0380015466.01.T01

Gene Sequence

>MsG0380015466.01.T01

ATGACGTTTCTTGCTTCAGGCCGCATAGTCATGGGCCTTTTTGGGACCACAGTACCGAAAACTGCAGAAAACTTCCGTGCACTTTGTACAGAATCACATTTCAGGAGAGAAAGGGTTGGAAAAAGTGGGAAGCCTCTTCATTATAAAGGGAGTGCATTCCATAGGATAATACCAAGCTTCATGGTTCAGGGTGGTGATTTCACCAGTGGTGATGGAAGAGGTGGAGAATCAATTTATGGAGATAAGTTTGCTGATGAAAACTTCAAGCTGAAGCATACTGGTCCAGGGTACTTATCAATGGCAAATTCTGGGCAAGATACCAATGGCTCACAGTTTTTCATAACAACAGTAAAAACTAGCTGGCTGGATGGACGCCATGTTGTATTCGGCAAGGTACTTTCTGGTATGGATGTTTTGTACAAGATTGAAGCAGAAGGAAGTGAAAGTGGCTCTCCCAAACGTACGGTTGTCATATTAGACAGTGGGGAATTAACTTCAGTTTAG

Protein sequence

>MsG0380015466.01.T01

MTFLASGRIVMGLFGTTVPKTAENFRALCTESHFRRERVGKSGKPLHYKGSAFHRIIPSFMVQGGDFTSGDGRGGESIYGDKFADENFKLKHTGPGYLSMANSGQDTNGSQFFITTVKTSWLDGRHVVFGKVLSGMDVLYKIEAEGSESGSPKRTVVILDSGELTSV*