AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040572.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040572.01.T01 MTR_7g093000 70.242 289 35 2 1 238 199 487 7.01e-128 373
MsG0780040572.01.T01 MTR_5g084810 32.484 157 105 1 82 238 347 502 4.80e-19 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040572.01.T01 AT5G06811 36.620 284 129 5 3 238 218 498 2.37e-44 157
MsG0780040572.01.T01 AT5G06810 33.929 280 138 6 3 238 226 502 1.36e-41 149
MsG0780040572.01.T01 AT4G19650 42.038 157 89 1 82 238 357 511 3.75e-27 109
MsG0780040572.01.T01 AT4G19650 42.038 157 89 1 82 238 340 494 4.43e-27 109
MsG0780040572.01.T01 AT5G45113 35.443 158 97 2 82 238 180 333 4.55e-21 91.3

Find 55 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAATGGATTGGGCGTTTA+AGG 0.166970 7:-79360130 MsG0780040572.01.T01:CDS
GAAATGAAGAACTGGGTTCT+TGG 0.309862 7:-79360258 MsG0780040572.01.T01:CDS
AGGTAAGGGAGCTGAGCTTC+AGG 0.325160 7:-79360100 MsG0780040572.01.T01:CDS
TTAAGCCTATGGTTGGCTTA+AGG 0.351833 7:-79360227 MsG0780040572.01.T01:CDS
ATCCTCTTGAAATGAAGAAC+TGG 0.352452 7:-79360266 MsG0780040572.01.T01:CDS
TAAGCCTATGGTTGGCTTAA+GGG 0.353016 7:-79360226 MsG0780040572.01.T01:CDS
GGCAAGACACTCTCGATGAT+TGG 0.410057 7:-79360549 MsG0780040572.01.T01:CDS
TTCTTAATCAGAACACAGAT+AGG 0.440115 7:-79360002 MsG0780040572.01.T01:CDS
CAGTCTCACACAGTACTTGC+GGG 0.440618 7:-79360372 MsG0780040572.01.T01:CDS
TATGGTTGGCTTAAGGGATC+TGG 0.446200 7:-79360220 MsG0780040572.01.T01:CDS
TGTGGAAAACACGAAAGAAA+TGG 0.448745 7:-79360145 MsG0780040572.01.T01:CDS
CTTGTTGCGCTTAGGTTATG+TGG 0.449939 7:-79360163 MsG0780040572.01.T01:CDS
GTTTAAGGCGTTCAGAGGTA+AGG 0.457273 7:-79360115 MsG0780040572.01.T01:CDS
GAGATGGAAGCTAAAGAGAT+TGG 0.461660 7:-79360405 MsG0780040572.01.T01:CDS
TGTTAATGTTGAATTTGTTA+AGG 0.462584 7:-79360780 MsG0780040572.01.T01:CDS
CCGCAAGTACTGTGTGAGAC+TGG 0.465962 7:+79360373 None:intergenic
GTTGCTTGAATGTAGGTAAG+AGG 0.469865 7:-79360314 MsG0780040572.01.T01:CDS
CGAGAAACAAGAGGGCAATC+TGG 0.474907 7:+79360498 None:intergenic
AAGACAACTAAGAGTTTGCT+TGG 0.477982 7:-79360336 MsG0780040572.01.T01:CDS
AAAACACGAAAGAAATGGAT+TGG 0.486098 7:-79360140 MsG0780040572.01.T01:CDS
TTGCTTGGTTGCTTGAATGT+AGG 0.497867 7:-79360321 MsG0780040572.01.T01:CDS
ACAGAGTTCTTGTTGCGCTT+AGG 0.497883 7:-79360171 MsG0780040572.01.T01:CDS
TGGAATAAGAGTTAAGCCTA+TGG 0.513478 7:-79360238 MsG0780040572.01.T01:CDS
ACCCAGTTCTTCATTTCAAG+AGG 0.514113 7:+79360264 None:intergenic
TTGAAGAATATTGTTGCAAA+GGG 0.516097 7:-79360746 MsG0780040572.01.T01:CDS
GCTTCTGACTGAAATTGAGA+TGG 0.523682 7:-79360421 MsG0780040572.01.T01:CDS
ACATCACCAACCACAATCCC+TGG 0.524076 7:+79360800 None:intergenic
ATTGTTGCAAAGGGTGGTGA+TGG 0.524388 7:-79360737 MsG0780040572.01.T01:CDS
TGGCTTAAGGGATCTGGAAG+AGG 0.530600 7:-79360214 MsG0780040572.01.T01:CDS
AAACACGAAAGAAATGGATT+GGG 0.533185 7:-79360139 MsG0780040572.01.T01:CDS
GAAGAGGAGAAATCGAGGGT+GGG 0.535364 7:-79360198 MsG0780040572.01.T01:CDS
TGCTGTAAGTCCAGGGATTG+TGG 0.540265 7:-79360810 MsG0780040572.01.T01:CDS
CGAGTTTCCACAGATTTCGA+TGG 0.543091 7:-79360477 MsG0780040572.01.T01:CDS
ATAAGAGTTAAGCCTATGGT+TGG 0.547914 7:-79360234 MsG0780040572.01.T01:CDS
AGATCCCTTAAGCCAACCAT+AGG 0.552682 7:+79360222 None:intergenic
AAGAATATTGTTGCAAAGGG+TGG 0.552691 7:-79360743 MsG0780040572.01.T01:CDS
GCGTCTATGTAGCATACTGC+AGG 0.554692 7:-79360292 MsG0780040572.01.T01:CDS
AAGAGGAGAAATCGAGGGTG+GGG 0.556838 7:-79360197 MsG0780040572.01.T01:CDS
GTTGAAGAATATTGTTGCAA+AGG 0.570077 7:-79360747 MsG0780040572.01.T01:CDS
AATGCTGTTGCTGTAAGTCC+AGG 0.570515 7:-79360818 MsG0780040572.01.T01:CDS
CCAGTCTCACACAGTACTTG+CGG 0.570636 7:-79360373 MsG0780040572.01.T01:CDS
TTTAAGGCGTTCAGAGGTAA+GGG 0.573608 7:-79360114 MsG0780040572.01.T01:CDS
GGAAGAGGAGAAATCGAGGG+TGG 0.579631 7:-79360199 MsG0780040572.01.T01:CDS
ATCTGGAAGAGGAGAAATCG+AGG 0.593033 7:-79360203 MsG0780040572.01.T01:CDS
TCCTCTTGAAATGAAGAACT+GGG 0.593818 7:-79360265 MsG0780040572.01.T01:CDS
TGGGCGTTTAAGGCGTTCAG+AGG 0.599598 7:-79360120 MsG0780040572.01.T01:CDS
TCTTAATCAGAACACAGATA+GGG 0.611252 7:-79360001 MsG0780040572.01.T01:CDS
GCTGAAGACACACCTCAAGT+TGG 0.614720 7:+79360442 None:intergenic
GTAAGTCCAGGGATTGTGGT+TGG 0.623479 7:-79360806 MsG0780040572.01.T01:CDS
GTGTTCTGATTAAGAATCTG+AGG 0.641643 7:+79360009 None:intergenic
GTGGAAACTCGAGAAACAAG+AGG 0.644988 7:+79360489 None:intergenic
TGGAAACTCGAGAAACAAGA+GGG 0.652528 7:+79360490 None:intergenic
ATGCTGTTGCTGTAAGTCCA+GGG 0.682252 7:-79360817 MsG0780040572.01.T01:CDS
AACTTGACCATCGAAATCTG+TGG 0.684694 7:+79360470 None:intergenic
TCTGGAAGAGGAGAAATCGA+GGG 0.689277 7:-79360202 MsG0780040572.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGATTTTATTGTTAATAC+TGG - Chr7:79360721-79360740 MsG0780040572.01.T01:intron 15.0%
!!! TGTATTATTTTTGAAGAATC+AGG - Chr7:79360220-79360239 MsG0780040572.01.T01:CDS 20.0%
!!! TGTTAATGTTGAATTTGTTA+AGG - Chr7:79360013-79360032 MsG0780040572.01.T01:CDS 20.0%
! TTGAAGAATATTGTTGCAAA+GGG - Chr7:79360047-79360066 MsG0780040572.01.T01:CDS 25.0%
!!! ATTATTTTTGAAGAATCAGG+AGG - Chr7:79360223-79360242 MsG0780040572.01.T01:CDS 25.0%
AAAACACGAAAGAAATGGAT+TGG - Chr7:79360653-79360672 MsG0780040572.01.T01:intron 30.0%
GTTGAAGAATATTGTTGCAA+AGG - Chr7:79360046-79360065 MsG0780040572.01.T01:CDS 30.0%
TCTTAATCAGAACACAGATA+GGG - Chr7:79360792-79360811 MsG0780040572.01.T01:CDS 30.0%
TTCTTAATCAGAACACAGAT+AGG - Chr7:79360791-79360810 MsG0780040572.01.T01:CDS 30.0%
! AAACACGAAAGAAATGGATT+GGG - Chr7:79360654-79360673 MsG0780040572.01.T01:intron 30.0%
!!! GATTGGTTTTTGGTCAAATT+TGG - Chr7:79360261-79360280 MsG0780040572.01.T01:CDS 30.0%
AAGAATATTGTTGCAAAGGG+TGG - Chr7:79360050-79360069 MsG0780040572.01.T01:CDS 35.0%
ATAAGAGTTAAGCCTATGGT+TGG - Chr7:79360559-79360578 MsG0780040572.01.T01:intron 35.0%
ATCCTCTTGAAATGAAGAAC+TGG - Chr7:79360527-79360546 MsG0780040572.01.T01:CDS 35.0%
GATGAGGTTTCTTGTAATTG+GGG - Chr7:79360116-79360135 MsG0780040572.01.T01:CDS 35.0%
GGATGAGGTTTCTTGTAATT+GGG - Chr7:79360115-79360134 MsG0780040572.01.T01:CDS 35.0%
GTGTTCTGATTAAGAATCTG+AGG + Chr7:79360787-79360806 None:intergenic 35.0%
TCCTCTTGAAATGAAGAACT+GGG - Chr7:79360528-79360547 MsG0780040572.01.T01:CDS 35.0%
TGGAATAAGAGTTAAGCCTA+TGG - Chr7:79360555-79360574 MsG0780040572.01.T01:intron 35.0%
TGGATGAGGTTTCTTGTAAT+TGG - Chr7:79360114-79360133 MsG0780040572.01.T01:CDS 35.0%
TGTGGAAAACACGAAAGAAA+TGG - Chr7:79360648-79360667 MsG0780040572.01.T01:intron 35.0%
! AAGACAACTAAGAGTTTGCT+TGG - Chr7:79360457-79360476 MsG0780040572.01.T01:CDS 35.0%
!!! ATGGTGATTTTGATAGTGGT+TGG - Chr7:79360075-79360094 MsG0780040572.01.T01:CDS 35.0%
!!! TCAAGTTTTTTGCCAACTTG+AGG - Chr7:79360339-79360358 MsG0780040572.01.T01:CDS 35.0%
!!! TTGGATTCAGTGGCATTTTT+TGG - Chr7:79360094-79360113 MsG0780040572.01.T01:CDS 35.0%
AACTTGACCATCGAAATCTG+TGG + Chr7:79360326-79360345 None:intergenic 40.0%
ACCCAGTTCTTCATTTCAAG+AGG + Chr7:79360532-79360551 None:intergenic 40.0%
GAAATGAAGAACTGGGTTCT+TGG - Chr7:79360535-79360554 MsG0780040572.01.T01:CDS 40.0%
GAGATGGAAGCTAAAGAGAT+TGG - Chr7:79360388-79360407 MsG0780040572.01.T01:CDS 40.0%
GCTTCTGACTGAAATTGAGA+TGG - Chr7:79360372-79360391 MsG0780040572.01.T01:CDS 40.0%
GTTGCTTGAATGTAGGTAAG+AGG - Chr7:79360479-79360498 MsG0780040572.01.T01:CDS 40.0%
TGGAAACTCGAGAAACAAGA+GGG + Chr7:79360306-79360325 None:intergenic 40.0%
TGGTTTCAGTGAGAAACAGT+TGG - Chr7:79360175-79360194 MsG0780040572.01.T01:CDS 40.0%
TTGCTTGGTTGCTTGAATGT+AGG - Chr7:79360472-79360491 MsG0780040572.01.T01:CDS 40.0%
TTGTGCTTGCTTTCGAATGT+TGG - Chr7:79360155-79360174 MsG0780040572.01.T01:CDS 40.0%
! TTTAAGGCGTTCAGAGGTAA+GGG - Chr7:79360679-79360698 MsG0780040572.01.T01:intron 40.0%
!! AGAAATGGATTGGGCGTTTA+AGG - Chr7:79360663-79360682 MsG0780040572.01.T01:intron 40.0%
!! GGTGATGGTGATTTTGATAG+TGG - Chr7:79360071-79360090 MsG0780040572.01.T01:CDS 40.0%
!! TAAGCCTATGGTTGGCTTAA+GGG - Chr7:79360567-79360586 MsG0780040572.01.T01:intron 40.0%
!! TTAAGCCTATGGTTGGCTTA+AGG - Chr7:79360566-79360585 MsG0780040572.01.T01:intron 40.0%
!! TTGATAGTGGTTGGATTCAG+TGG - Chr7:79360084-79360103 MsG0780040572.01.T01:CDS 40.0%
!!! CACTCTCGATGATTGGTTTT+TGG - Chr7:79360251-79360270 MsG0780040572.01.T01:CDS 40.0%
!!! TCAGTGGCATTTTTTGGATG+AGG - Chr7:79360100-79360119 MsG0780040572.01.T01:CDS 40.0%
AATGCTGTTGCTGTAAGTCC+AGG - Chr7:79359975-79359994 MsG0780040572.01.T01:CDS 45.0%
ACAGAGTTCTTGTTGCGCTT+AGG - Chr7:79360622-79360641 MsG0780040572.01.T01:intron 45.0%
AGATCCCTTAAGCCAACCAT+AGG + Chr7:79360574-79360593 None:intergenic 45.0%
ATCTGGAAGAGGAGAAATCG+AGG - Chr7:79360590-79360609 MsG0780040572.01.T01:intron 45.0%
ATGCTGTTGCTGTAAGTCCA+GGG - Chr7:79359976-79359995 MsG0780040572.01.T01:CDS 45.0%
ATTGTTGCAAAGGGTGGTGA+TGG - Chr7:79360056-79360075 MsG0780040572.01.T01:CDS 45.0%
CAGTTGGCTGAGATTATTCG+TGG - Chr7:79360191-79360210 MsG0780040572.01.T01:CDS 45.0%
CGAGTTTCCACAGATTTCGA+TGG - Chr7:79360316-79360335 MsG0780040572.01.T01:CDS 45.0%
CTTGTTGCGCTTAGGTTATG+TGG - Chr7:79360630-79360649 MsG0780040572.01.T01:intron 45.0%
GTGGAAACTCGAGAAACAAG+AGG + Chr7:79360307-79360326 None:intergenic 45.0%
TCTGGAAGAGGAGAAATCGA+GGG - Chr7:79360591-79360610 MsG0780040572.01.T01:intron 45.0%
! GTTTAAGGCGTTCAGAGGTA+AGG - Chr7:79360678-79360697 MsG0780040572.01.T01:intron 45.0%
!! TATGGTTGGCTTAAGGGATC+TGG - Chr7:79360573-79360592 MsG0780040572.01.T01:intron 45.0%
AAGAGGAGAAATCGAGGGTG+GGG - Chr7:79360596-79360615 MsG0780040572.01.T01:intron 50.0%
ACATCACCAACCACAATCCC+TGG + Chr7:79359996-79360015 None:intergenic 50.0%
CAGTCTCACACAGTACTTGC+GGG - Chr7:79360421-79360440 MsG0780040572.01.T01:CDS 50.0%
CCAGTCTCACACAGTACTTG+CGG - Chr7:79360420-79360439 MsG0780040572.01.T01:CDS 50.0%
CGAGAAACAAGAGGGCAATC+TGG + Chr7:79360298-79360317 None:intergenic 50.0%
GAAGAGGAGAAATCGAGGGT+GGG - Chr7:79360595-79360614 MsG0780040572.01.T01:intron 50.0%
GCGTCTATGTAGCATACTGC+AGG - Chr7:79360501-79360520 MsG0780040572.01.T01:CDS 50.0%
GCTGAAGACACACCTCAAGT+TGG + Chr7:79360354-79360373 None:intergenic 50.0%
GGCAAGACACTCTCGATGAT+TGG - Chr7:79360244-79360263 MsG0780040572.01.T01:CDS 50.0%
GTAAGTCCAGGGATTGTGGT+TGG - Chr7:79359987-79360006 MsG0780040572.01.T01:CDS 50.0%
TGCTGTAAGTCCAGGGATTG+TGG - Chr7:79359983-79360002 MsG0780040572.01.T01:CDS 50.0%
TGGCTTAAGGGATCTGGAAG+AGG - Chr7:79360579-79360598 MsG0780040572.01.T01:intron 50.0%
AGGTAAGGGAGCTGAGCTTC+AGG - Chr7:79360693-79360712 MsG0780040572.01.T01:intron 55.0%
GGAAGAGGAGAAATCGAGGG+TGG - Chr7:79360594-79360613 MsG0780040572.01.T01:intron 55.0%
! CCGCAAGTACTGTGTGAGAC+TGG + Chr7:79360423-79360442 None:intergenic 55.0%
! TGGGCGTTTAAGGCGTTCAG+AGG - Chr7:79360673-79360692 MsG0780040572.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 79359975 79360840 79359975 ID=MsG0780040572.01;Name=MsG0780040572.01
Chr7 mRNA 79359975 79360840 79359975 ID=MsG0780040572.01.T01;Parent=MsG0780040572.01;Name=MsG0780040572.01.T01;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|1|0.5|2|0|238
Chr7 exon 79360723 79360840 79360723 ID=MsG0780040572.01.T01:exon:16279;Parent=MsG0780040572.01.T01
Chr7 exon 79359975 79360573 79359975 ID=MsG0780040572.01.T01:exon:16278;Parent=MsG0780040572.01.T01
Chr7 CDS 79360723 79360840 79360723 ID=MsG0780040572.01.T01:cds;Parent=MsG0780040572.01.T01
Chr7 CDS 79359975 79360573 79359975 ID=MsG0780040572.01.T01:cds;Parent=MsG0780040572.01.T01
Gene Sequence

>MsG0780040572.01.T01

AATGCTGTTGCTGTAAGTCCAGGGATTGTGGTTGGTGATGTTAATGTTGAATTTGTTAAGGTTGTTGAGATGTTGAAGAATATTGTTGCAAAGGGTGGTGATGGTGATTTTGATAGTGGAGGCAAGACACTCTCGATGATTGGTTTTTGGTCAAATTTGGATGTCTGTGAACCAGATTGCCCTCTTGTTTCTCGAGTTTCCACAGATTTCGATGGTCAAGTTTTTTGCCAACTTGAGGTGTGTCTTCAGCTTCTGACTGAAATTGAGATGGAAGCTAAAGAGATTGGAAAGATTTTCCAGTCTCACACAGTACTTGCGGGTTCAAATACTCTAAAGACAACTAAGAGTTTGCTTGGTTGCTTGAATGTAGGTAAGAGGCGTCTATGTAGCATACTGCAGGATAATCCTCTTGAAATGAAGAACTGGGTTCTTGGAATAAGAGTTAAGCCTATGGTTGGCTTAAGGGATCTGGAAGAGGAGAAATCGAGGGTGGGGAAGACAGAGTTCTTGTTGCGCTTAGGTTATGTGGAAAACACGAAAGAAATGGATTGGGCGTTTAAGGCGTTCAGAGGTAAGGGAGCTGAGCTTCAGGAGAGATTTGATTTTATTGTTAATACTGGATTGACACGTGATGAAGTTCGTAGAATGATTAGAGTTTCACCTCAGATTCTTAATCAGAACACAGATAGGGTCAAGATGAAAATGAGTATCTTGTAA

Protein sequence

>MsG0780040572.01.T01

NAVAVSPGIVVGDVNVEFVKVVEMLKNIVAKGGDGDFDSGGKTLSMIGFWSNLDVCEPDCPLVSRVSTDFDGQVFCQLEVCLQLLTEIEMEAKEIGKIFQSHTVLAGSNTLKTTKSLLGCLNVGKRRLCSILQDNPLEMKNWVLGIRVKPMVGLRDLEEEKSRVGKTEFLLRLGYVENTKEMDWAFKAFRGKGAELQERFDFIVNTGLTRDEVRRMIRVSPQILNQNTDRVKMKMSIL*