Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033619.01.T01 | KEH25700.1 | 51.22 | 205 | 36 | 6 | 1 | 141 | 262 | 466 | 1.39E-46 | 167 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033619.01.T01 | Q689D6 | 70.526 | 95 | 28 | 0 | 1 | 95 | 262 | 356 | 4.04E-40 | 141 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033619.01.T01 | A0A072UIR4 | 51.220 | 205 | 36 | 6 | 1 | 141 | 262 | 466 | 6.63e-47 | 167 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047957.01 | MsG0680033619.01 | 0.800821 | 1.204108e-48 | 5.661272e-46 |
| MsG0080047959.01 | MsG0680033619.01 | 0.808097 | 3.660904e-50 | 2.074561e-47 |
| MsG0080048461.01 | MsG0680033619.01 | 0.801302 | 9.598571e-49 | 4.568328e-46 |
| MsG0080048856.01 | MsG0680033619.01 | 0.807653 | 4.550181e-50 | 2.548508e-47 |
| MsG0180000144.01 | MsG0680033619.01 | 0.800296 | 1.540205e-48 | 7.146111e-46 |
| MsG0180000195.01 | MsG0680033619.01 | 0.803612 | 3.207784e-49 | 1.619227e-46 |
| MsG0180000235.01 | MsG0680033619.01 | 0.817589 | 3.049354e-52 | 2.229835e-49 |
| MsG0180000470.01 | MsG0680033619.01 | 0.821910 | 3.140399e-53 | 2.589225e-50 |
| MsG0180000555.01 | MsG0680033619.01 | 0.819654 | 1.036606e-52 | 8.025358e-50 |
| MsG0180000732.01 | MsG0680033619.01 | 0.808911 | 2.454276e-50 | 1.420757e-47 |
| MsG0180000836.01 | MsG0680033619.01 | 0.800962 | 1.126931e-48 | 5.317763e-46 |
| MsG0180001141.01 | MsG0680033619.01 | 0.814114 | 1.816567e-51 | 1.208223e-48 |
| MsG0180001312.01 | MsG0680033619.01 | 0.803471 | 3.431449e-49 | 1.725847e-46 |
| MsG0180001381.01 | MsG0680033619.01 | 0.814397 | 1.572890e-51 | 1.054283e-48 |
| MsG0180001570.01 | MsG0680033619.01 | 0.817267 | 3.603085e-52 | 2.611557e-49 |
| MsG0180002283.01 | MsG0680033619.01 | 0.801797 | 7.598982e-49 | 3.662119e-46 |
| MsG0180003073.01 | MsG0680033619.01 | 0.808575 | 2.895302e-50 | 1.661374e-47 |
| MsG0180003097.01 | MsG0680033619.01 | 0.800569 | 1.355525e-48 | 6.332563e-46 |
| MsG0180003669.01 | MsG0680033619.01 | 0.800984 | 1.114996e-48 | 5.264530e-46 |
| MsG0180003810.01 | MsG0680033619.01 | 0.814204 | 1.735081e-51 | 1.156830e-48 |
| MsG0180003854.01 | MsG0680033619.01 | 0.831774 | 1.379167e-55 | 1.511931e-52 |
| MsG0180004372.01 | MsG0680033619.01 | 0.801077 | 1.067189e-48 | 5.050587e-46 |
| MsG0180004449.01 | MsG0680033619.01 | 0.803664 | 3.129164e-49 | 1.581591e-46 |
| MsG0180004926.01 | MsG0680033619.01 | 0.806926 | 6.489425e-50 | 3.566670e-47 |
| MsG0180005050.01 | MsG0680033619.01 | 0.831174 | 1.938715e-55 | 2.087365e-52 |
| MsG0180005786.01 | MsG0680033619.01 | 0.809870 | 1.528121e-50 | 9.075253e-48 |
| MsG0180005993.01 | MsG0680033619.01 | 0.803059 | 4.176317e-49 | 2.078287e-46 |
| MsG0180006143.01 | MsG0680033619.01 | 0.811298 | 7.507849e-51 | 4.630126e-48 |
| MsG0280006406.01 | MsG0680033619.01 | 0.815355 | 9.645550e-52 | 6.635080e-49 |
| MsG0280011334.01 | MsG0680033619.01 | 0.803721 | 3.045901e-49 | 1.541758e-46 |
| MsG0380011661.01 | MsG0680033619.01 | 0.804963 | 1.678419e-49 | 8.772391e-47 |
| MsG0380011716.01 | MsG0680033619.01 | 0.809698 | 1.664205e-50 | 9.838876e-48 |
| MsG0380012057.01 | MsG0680033619.01 | 0.820718 | 5.916535e-53 | 4.717708e-50 |
| MsG0380013834.01 | MsG0680033619.01 | 0.814561 | 1.447416e-51 | 9.744534e-49 |
| MsG0380014701.01 | MsG0680033619.01 | 0.809766 | 1.609134e-50 | 9.530804e-48 |
| MsG0380014770.01 | MsG0680033619.01 | 0.807622 | 4.618966e-50 | 2.585004e-47 |
| MsG0380014885.01 | MsG0680033619.01 | 0.806227 | 9.111863e-50 | 4.919389e-47 |
| MsG0680030753.01 | MsG0680033619.01 | 0.808887 | 2.483668e-50 | 1.436829e-47 |
| MsG0680031262.01 | MsG0680033619.01 | 0.810592 | 1.067524e-50 | 6.461412e-48 |
| MsG0680032430.01 | MsG0680033619.01 | 0.807308 | 5.385067e-50 | 2.989409e-47 |
| MsG0680033391.01 | MsG0680033619.01 | 0.804548 | 2.049028e-49 | 1.059457e-46 |
| MsG0680033392.01 | MsG0680033619.01 | 0.807592 | 4.687542e-50 | 2.621219e-47 |
| MsG0680033619.01 | MsG0680034739.01 | 0.808879 | 2.492519e-50 | 1.441680e-47 |
| MsG0680033619.01 | MsG0680035278.01 | 0.824368 | 8.383722e-54 | 7.410812e-51 |
| MsG0680033619.01 | MsG0680035346.01 | 0.806178 | 9.332531e-50 | 5.032135e-47 |
| MsG0680033619.01 | MsG0680035704.01 | 0.801174 | 1.019646e-48 | 4.837206e-46 |
| MsG0680033619.01 | MsG0780035948.01 | 0.813751 | 2.184465e-51 | 1.438661e-48 |
| MsG0680033619.01 | MsG0780036609.01 | 0.804592 | 2.006616e-49 | 1.038722e-46 |
| MsG0680033619.01 | MsG0780036719.01 | 0.815638 | 8.345709e-52 | 5.785871e-49 |
| MsG0680033619.01 | MsG0780039144.01 | 0.802335 | 5.890263e-49 | 2.877884e-46 |
| MsG0680033619.01 | MsG0780039952.01 | 0.832199 | 1.083224e-55 | 1.202150e-52 |
| MsG0680033619.01 | MsG0780040445.01 | 0.853325 | 2.574829e-61 | 5.542610e-58 |
| MsG0680033619.01 | MsG0780040572.01 | 0.829017 | 6.513830e-55 | 6.584197e-52 |
| MsG0680033619.01 | MsG0780040861.01 | 0.815631 | 8.371932e-52 | 5.803151e-49 |
| MsG0680033619.01 | MsG0780041065.01 | 0.815844 | 7.504746e-52 | 5.231825e-49 |
| MsG0680033619.01 | MsG0780041294.01 | 0.803166 | 3.968639e-49 | 1.980477e-46 |
| MsG0680033619.01 | MsG0780041469.01 | 0.803344 | 3.646122e-49 | 1.827773e-46 |
| MsG0680033619.01 | MsG0780041559.01 | 0.822146 | 2.768915e-53 | 2.298313e-50 |
| MsG0680033619.01 | MsG0880042314.01 | 0.805937 | 1.048690e-49 | 5.619860e-47 |
| MsG0680033619.01 | MsG0880042404.01 | 0.821250 | 4.461346e-53 | 3.610516e-50 |
| MsG0680033619.01 | MsG0880042518.01 | 0.824227 | 9.049444e-54 | 7.967391e-51 |
| MsG0680033619.01 | MsG0880042792.01 | 0.803421 | 3.513644e-49 | 1.764947e-46 |
| MsG0680033619.01 | MsG0880043059.01 | 0.826091 | 3.280424e-54 | 3.046091e-51 |
| MsG0680033619.01 | MsG0880044237.01 | 0.808274 | 3.357255e-50 | 1.911447e-47 |
| MsG0680033619.01 | MsG0880044574.01 | 0.805150 | 1.533686e-49 | 8.054387e-47 |
| MsG0680033619.01 | MsG0880045736.01 | 0.819831 | 9.445555e-53 | 7.348888e-50 |
| MsG0680033619.01 | MsG0880046285.01 | 0.825821 | 3.802720e-54 | 3.504260e-51 |
| MsG0680033619.01 | MsG0880046321.01 | 0.818985 | 1.472401e-52 | 1.119072e-49 |
| MsG0680033619.01 | MsG0880046973.01 | 0.810668 | 1.028239e-50 | 6.236343e-48 |
| MsG0680033619.01 | MsG0880047019.01 | 0.804284 | 2.326058e-49 | 1.194569e-46 |
| MsG0680033619.01 | MsG0880047038.01 | 0.822473 | 2.324734e-53 | 1.947808e-50 |
| MsG0480019373.01 | MsG0680033619.01 | 0.826313 | 2.905864e-54 | 2.715479e-51 |
| MsG0480019970.01 | MsG0680033619.01 | 0.839681 | 1.369800e-57 | 1.905764e-54 |
| MsG0480021274.01 | MsG0680033619.01 | 0.807263 | 5.506056e-50 | 3.052896e-47 |
| MsG0480021724.01 | MsG0680033619.01 | 0.801622 | 8.255039e-49 | 3.960512e-46 |
| MsG0480021853.01 | MsG0680033619.01 | 0.812200 | 4.778582e-51 | 3.018942e-48 |
| MsG0480021994.01 | MsG0680033619.01 | 0.807816 | 4.202140e-50 | 2.363630e-47 |
| MsG0480022318.01 | MsG0680033619.01 | 0.801362 | 9.331360e-49 | 4.447916e-46 |
| MsG0480022615.01 | MsG0680033619.01 | -0.812567 | 3.972042e-51 | 2.534116e-48 |
| MsG0480022768.01 | MsG0680033619.01 | 0.802078 | 6.652780e-49 | 3.229108e-46 |
| MsG0480022791.01 | MsG0680033619.01 | 0.826285 | 2.950360e-54 | 2.754893e-51 |
| MsG0480022851.01 | MsG0680033619.01 | 0.812961 | 3.257187e-51 | 2.099637e-48 |
| MsG0480022985.01 | MsG0680033619.01 | 0.813401 | 2.607908e-51 | 1.701415e-48 |
| MsG0480022986.01 | MsG0680033619.01 | 0.827602 | 1.428869e-54 | 1.385889e-51 |
| MsG0480023014.01 | MsG0680033619.01 | 0.801564 | 8.482175e-49 | 4.063678e-46 |
| MsG0480023415.01 | MsG0680033619.01 | 0.806723 | 7.161164e-50 | 3.915380e-47 |
| MsG0480023659.01 | MsG0680033619.01 | 0.809930 | 1.483513e-50 | 8.824195e-48 |
| MsG0480023718.01 | MsG0680033619.01 | 0.806250 | 9.011250e-50 | 4.867876e-47 |
| MsG0480023943.01 | MsG0680033619.01 | 0.819685 | 1.019964e-52 | 7.903067e-50 |
| MsG0580024114.01 | MsG0680033619.01 | 0.802745 | 4.849511e-49 | 2.394228e-46 |
| MsG0580024165.01 | MsG0680033619.01 | 0.822298 | 2.553536e-53 | 2.128842e-50 |
| MsG0580024274.01 | MsG0680033619.01 | 0.816327 | 5.854266e-52 | 4.135919e-49 |
| MsG0580024304.01 | MsG0680033619.01 | 0.830255 | 3.254964e-55 | 3.411452e-52 |
| MsG0580024374.01 | MsG0680033619.01 | 0.800838 | 1.194252e-48 | 5.617600e-46 |
| MsG0580024390.01 | MsG0680033619.01 | 0.813197 | 2.891224e-51 | 1.875642e-48 |
| MsG0580024733.01 | MsG0680033619.01 | 0.816487 | 5.390941e-52 | 3.824906e-49 |
| MsG0580024946.01 | MsG0680033619.01 | 0.806691 | 7.272762e-50 | 3.973012e-47 |
| MsG0580025421.01 | MsG0680033619.01 | 0.802096 | 6.596982e-49 | 3.203509e-46 |
| MsG0580025448.01 | MsG0680033619.01 | 0.827527 | 1.489366e-54 | 1.441501e-51 |
| MsG0580025621.01 | MsG0680033619.01 | 0.814794 | 1.284879e-51 | 8.704563e-49 |
| MsG0380015164.01 | MsG0680033619.01 | 0.802283 | 6.037702e-49 | 2.946052e-46 |
| MsG0380015300.01 | MsG0680033619.01 | 0.805131 | 1.548041e-49 | 8.125762e-47 |
| MsG0380015440.01 | MsG0680033619.01 | 0.817169 | 3.790541e-52 | 2.740203e-49 |
| MsG0380015831.01 | MsG0680033619.01 | 0.804148 | 2.482852e-49 | 1.270665e-46 |
| MsG0380016971.01 | MsG0680033619.01 | 0.800034 | 1.741179e-48 | 8.024681e-46 |
| MsG0380017266.01 | MsG0680033619.01 | -0.802985 | 4.325583e-49 | 2.148527e-46 |
| MsG0380017551.01 | MsG0680033619.01 | -0.823352 | 1.450938e-53 | 1.246167e-50 |
| MsG0380017680.01 | MsG0680033619.01 | 0.809203 | 2.124615e-50 | 1.239697e-47 |
| MsG0380017864.01 | MsG0680033619.01 | 0.819694 | 1.015338e-52 | 7.869259e-50 |
| MsG0380018009.01 | MsG0680033619.01 | 0.817958 | 2.517486e-52 | 1.859622e-49 |
| MsG0480018163.01 | MsG0680033619.01 | 0.822439 | 2.368167e-53 | 1.982234e-50 |
| MsG0580026439.01 | MsG0680033619.01 | 0.807573 | 4.732343e-50 | 2.644982e-47 |
| MsG0580028991.01 | MsG0680033619.01 | 0.809701 | 1.661435e-50 | 9.823311e-48 |
| MsG0580029008.01 | MsG0680033619.01 | 0.806484 | 8.042698e-50 | 4.370520e-47 |
| MsG0580029651.01 | MsG0680033619.01 | 0.822196 | 2.695755e-53 | 2.240714e-50 |
| MsG0580029704.01 | MsG0680033619.01 | 0.802592 | 5.214259e-49 | 2.564295e-46 |
| MsG0580029762.01 | MsG0680033619.01 | 0.814269 | 1.678777e-51 | 1.121329e-48 |
| MsG0680030351.01 | MsG0680033619.01 | 0.823314 | 1.481028e-53 | 1.270720e-50 |
| MsG0680030413.01 | MsG0680033619.01 | 0.803166 | 3.969117e-49 | 1.980705e-46 |
| MsG0680030597.01 | MsG0680033619.01 | 0.813531 | 2.442377e-51 | 1.599047e-48 |
| MsG0280007064.01 | MsG0680033619.01 | 0.809826 | 1.561405e-50 | 9.262380e-48 |
| MsG0280007088.01 | MsG0680033619.01 | 0.805261 | 1.454194e-49 | 7.658113e-47 |
| MsG0280007222.01 | MsG0680033619.01 | 0.819063 | 1.413496e-52 | 1.076490e-49 |
| MsG0280007636.01 | MsG0680033619.01 | 0.820874 | 5.445952e-53 | 4.361861e-50 |
| MsG0280007727.01 | MsG0680033619.01 | 0.801577 | 8.431396e-49 | 4.040672e-46 |
| MsG0280007745.01 | MsG0680033619.01 | 0.808382 | 3.183844e-50 | 1.817831e-47 |
| MsG0280008615.01 | MsG0680033619.01 | 0.821319 | 4.301850e-53 | 3.488140e-50 |
| MsG0280008696.01 | MsG0680033619.01 | 0.810543 | 1.094196e-50 | 6.613853e-48 |
| MsG0280008907.01 | MsG0680033619.01 | 0.806132 | 9.543164e-50 | 5.139494e-47 |
| MsG0280008970.01 | MsG0680033619.01 | 0.817623 | 2.995476e-52 | 2.192530e-49 |
| MsG0280009024.01 | MsG0680033619.01 | 0.801376 | 9.270000e-49 | 4.420143e-46 |
| MsG0280009739.01 | MsG0680033619.01 | 0.806269 | 8.928245e-50 | 4.825430e-47 |
| MsG0280009748.01 | MsG0680033619.01 | 0.814781 | 1.293799e-51 | 8.761729e-49 |
| MsG0280010233.01 | MsG0680033619.01 | 0.801777 | 7.672431e-49 | 3.695545e-46 |
| MsG0280010443.01 | MsG0680033619.01 | 0.800790 | 1.221585e-48 | 5.738811e-46 |
| MsG0280010531.01 | MsG0680033619.01 | 0.805175 | 1.515183e-49 | 7.962296e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033619.01.T01 | MTR_6g033595 | 51.220 | 205 | 36 | 6 | 1 | 141 | 262 | 466 | 1.68e-50 | 167 |
| MsG0680033619.01.T01 | MTR_2g067960 | 49.268 | 205 | 40 | 6 | 1 | 141 | 227 | 431 | 2.97e-46 | 155 |
| MsG0680033619.01.T01 | MTR_6g033585 | 94.595 | 37 | 2 | 0 | 105 | 141 | 137 | 173 | 3.13e-16 | 71.6 |
| MsG0680033619.01.T01 | MTR_0020s0170 | 97.222 | 36 | 1 | 0 | 105 | 140 | 24 | 59 | 4.37e-16 | 68.2 |
| MsG0680033619.01.T01 | MTR_3g105530 | 35.802 | 162 | 67 | 8 | 4 | 134 | 257 | 412 | 1.86e-12 | 63.5 |
| MsG0680033619.01.T01 | MTR_3g029990 | 36.082 | 97 | 48 | 2 | 3 | 89 | 223 | 315 | 1.55e-11 | 61.2 |
| MsG0680033619.01.T01 | MTR_3g029990 | 36.458 | 96 | 47 | 2 | 4 | 89 | 224 | 315 | 1.71e-11 | 60.8 |
| MsG0680033619.01.T01 | MTR_3g030020 | 36.458 | 96 | 47 | 2 | 4 | 89 | 181 | 272 | 2.03e-11 | 60.5 |
| MsG0680033619.01.T01 | MTR_1g019170 | 36.458 | 96 | 47 | 2 | 4 | 89 | 224 | 315 | 2.09e-11 | 60.5 |
| MsG0680033619.01.T01 | MTR_3g063230 | 51.786 | 56 | 27 | 0 | 4 | 59 | 272 | 327 | 2.67e-11 | 60.5 |
| MsG0680033619.01.T01 | MTR_3g063230 | 51.786 | 56 | 27 | 0 | 4 | 59 | 248 | 303 | 2.78e-11 | 60.1 |
| MsG0680033619.01.T01 | MTR_1g098960 | 35.417 | 96 | 48 | 2 | 4 | 89 | 226 | 317 | 6.16e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033619.01.T01 | AT4G33495 | 70.526 | 95 | 28 | 0 | 1 | 95 | 262 | 356 | 4.12e-41 | 141 |
| MsG0680033619.01.T01 | AT5G45790 | 43.011 | 93 | 42 | 4 | 4 | 94 | 196 | 279 | 2.00e-12 | 63.5 |
| MsG0680033619.01.T01 | AT1G06440 | 55.357 | 56 | 24 | 1 | 4 | 59 | 257 | 311 | 2.84e-12 | 63.2 |
| MsG0680033619.01.T01 | AT5G45790 | 43.011 | 93 | 42 | 4 | 4 | 94 | 281 | 364 | 2.86e-12 | 63.2 |
| MsG0680033619.01.T01 | AT5G21970 | 40.000 | 95 | 43 | 3 | 4 | 88 | 271 | 361 | 1.36e-11 | 61.2 |
| MsG0680033619.01.T01 | AT5G21970 | 40.000 | 95 | 43 | 3 | 4 | 88 | 271 | 361 | 1.36e-11 | 61.2 |
Find 29 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACCTTATGAGGATGTTTC+GGG | 0.240740 | 6:-69612029 | MsG0680033619.01.T01:CDS |
| GTCACCTTATGAGGATGTTT+CGG | 0.256859 | 6:-69612030 | MsG0680033619.01.T01:CDS |
| AGTGGCAAAGGTTACCTTAT+TGG | 0.325465 | 6:-69612052 | MsG0680033619.01.T01:CDS |
| ACCTTATTGGTCACCTTATG+AGG | 0.335154 | 6:-69612039 | MsG0680033619.01.T01:CDS |
| GATGGTTTGTCGTCAGATAT+TGG | 0.346608 | 6:-69611541 | MsG0680033619.01.T01:CDS |
| TCCTCATAAGGTGACCAATA+AGG | 0.368472 | 6:+69612038 | None:intergenic |
| CAAAACTCCTTCAACTTCTT+CGG | 0.393749 | 6:+69611867 | None:intergenic |
| CGCAGAAGAGAATGGAGAAA+AGG | 0.413764 | 6:-69611983 | MsG0680033619.01.T01:CDS |
| ATGTTTCGGGGTATGATTTG+AGG | 0.434955 | 6:-69612016 | MsG0680033619.01.T01:CDS |
| GGCTTATAGGAAAGGTGAGT+TGG | 0.481467 | 6:-69611770 | MsG0680033619.01.T01:intron |
| GTTTCTACCAGAGGGAATCA+TGG | 0.486926 | 6:-69611820 | MsG0680033619.01.T01:CDS |
| TTAGGATTGCAATGTGGAAG+TGG | 0.497748 | 6:-69612070 | None:intergenic |
| GCAGAAGAGAATGGAGAAAA+GGG | 0.500751 | 6:-69611982 | MsG0680033619.01.T01:CDS |
| CTTAGGGAGGCTTATAGGAA+AGG | 0.506493 | 6:-69611778 | MsG0680033619.01.T01:CDS |
| AAGCTTCCCATGATTCCCTC+TGG | 0.520335 | 6:+69611813 | None:intergenic |
| GATTGAGGCGCAGAAGAGAA+TGG | 0.521729 | 6:-69611991 | MsG0680033619.01.T01:CDS |
| GTATGATTTGAGGTCGATTG+AGG | 0.524825 | 6:-69612006 | MsG0680033619.01.T01:CDS |
| GAACTTGCCGAAGAAGTTGA+AGG | 0.527122 | 6:-69611874 | MsG0680033619.01.T01:CDS |
| AGAAGAAGATTACGTTGGAG+AGG | 0.535460 | 6:-69611920 | MsG0680033619.01.T01:CDS |
| TGATAGTGTGTCAAATGAAA+AGG | 0.538090 | 6:-69611479 | MsG0680033619.01.T01:CDS |
| CAAGGTGAGGATGAAGAAGA+TGG | 0.538217 | 6:-69611559 | MsG0680033619.01.T01:CDS |
| TACCCCGAAACATCCTCATA+AGG | 0.539481 | 6:+69612026 | None:intergenic |
| TGATGTTGATTCTGCAAATG+AGG | 0.551897 | 6:-69611515 | MsG0680033619.01.T01:CDS |
| TTGCAATGTGGAAGTGGCAA+AGG | 0.564702 | 6:-69612064 | None:intergenic |
| TTTCTACCAGAGGGAATCAT+GGG | 0.570000 | 6:-69611819 | MsG0680033619.01.T01:CDS |
| TGGATCGTGTAGGCAAGGTG+AGG | 0.600927 | 6:-69611572 | MsG0680033619.01.T01:intron |
| TGTTGAGAAGAAGATTACGT+TGG | 0.617217 | 6:-69611925 | MsG0680033619.01.T01:CDS |
| TGACAACAATTCATGAATCG+TGG | 0.625232 | 6:+69611953 | None:intergenic |
| CACCTTATGAGGATGTTTCG+GGG | 0.687439 | 6:-69612028 | MsG0680033619.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGCAAGTTAGATGATGAAAT+GGG | - | Chr6:69611883-69611902 | MsG0680033619.01.T01:CDS | 30.0% | |
| TTGAGCCTAATGATTGTATT+TGG | - | Chr6:69611780-69611799 | MsG0680033619.01.T01:CDS | 30.0% | |
| ! | ATGCTTGTGAAGATTTTAAG+GGG | - | Chr6:69611932-69611951 | MsG0680033619.01.T01:CDS | 30.0% |
| ! | GATGCTTGTGAAGATTTTAA+GGG | - | Chr6:69611931-69611950 | MsG0680033619.01.T01:CDS | 30.0% |
| ! | TGATAGTGTGTCAAATGAAA+AGG | - | Chr6:69612048-69612067 | MsG0680033619.01.T01:CDS | 30.0% |
| !! | ATTTTCTATGTTTCTACCAG+AGG | - | Chr6:69611698-69611717 | MsG0680033619.01.T01:intron | 30.0% |
| !! | TTTTCTATGTTTCTACCAGA+GGG | - | Chr6:69611699-69611718 | MsG0680033619.01.T01:intron | 30.0% |
| CAAAACTCCTTCAACTTCTT+CGG | + | Chr6:69611663-69611682 | None:intergenic | 35.0% | |
| GAGCAAGTTAGATGATGAAA+TGG | - | Chr6:69611882-69611901 | MsG0680033619.01.T01:CDS | 35.0% | |
| GCAAGTTAGATGATGAAATG+GGG | - | Chr6:69611884-69611903 | MsG0680033619.01.T01:CDS | 35.0% | |
| TGACAACAATTCATGAATCG+TGG | + | Chr6:69611577-69611596 | None:intergenic | 35.0% | |
| TGTTGAGAAGAAGATTACGT+TGG | - | Chr6:69611602-69611621 | MsG0680033619.01.T01:intron | 35.0% | |
| ! | GGATGCTTGTGAAGATTTTA+AGG | - | Chr6:69611930-69611949 | MsG0680033619.01.T01:CDS | 35.0% |
| ! | TTTTTCTTAGGGAGGCTTAT+AGG | - | Chr6:69611744-69611763 | MsG0680033619.01.T01:intron | 35.0% |
| !! | AGCTTCACACTGTTTTTCTT+AGG | - | Chr6:69611732-69611751 | MsG0680033619.01.T01:intron | 35.0% |
| !! | GCTTCACACTGTTTTTCTTA+GGG | - | Chr6:69611733-69611752 | MsG0680033619.01.T01:intron | 35.0% |
| !! | TGATGTTGATTCTGCAAATG+AGG | - | Chr6:69612012-69612031 | MsG0680033619.01.T01:CDS | 35.0% |
| AATGATTGTATTTGGCGCGA+CGG | - | Chr6:69611788-69611807 | MsG0680033619.01.T01:CDS | 40.0% | |
| ACCTTATTGGTCACCTTATG+AGG | - | Chr6:69611488-69611507 | MsG0680033619.01.T01:CDS | 40.0% | |
| AGAAGAAGATTACGTTGGAG+AGG | - | Chr6:69611607-69611626 | MsG0680033619.01.T01:intron | 40.0% | |
| AGTGGCAAAGGTTACCTTAT+TGG | - | Chr6:69611475-69611494 | MsG0680033619.01.T01:CDS | 40.0% | |
| ATAGGGAATTGGTTGGGTAT+AGG | - | Chr6:69611857-69611876 | MsG0680033619.01.T01:CDS | 40.0% | |
| ATGTTTCGGGGTATGATTTG+AGG | - | Chr6:69611511-69611530 | MsG0680033619.01.T01:CDS | 40.0% | |
| GCAGAAGAGAATGGAGAAAA+GGG | - | Chr6:69611545-69611564 | MsG0680033619.01.T01:CDS | 40.0% | |
| GTCACCTTATGAGGATGTTT+CGG | - | Chr6:69611497-69611516 | MsG0680033619.01.T01:CDS | 40.0% | |
| TCACCTTATGAGGATGTTTC+GGG | - | Chr6:69611498-69611517 | MsG0680033619.01.T01:CDS | 40.0% | |
| TCCTCATAAGGTGACCAATA+AGG | + | Chr6:69611492-69611511 | None:intergenic | 40.0% | |
| TCGCGCCAAATACAATCATT+AGG | + | Chr6:69611788-69611807 | None:intergenic | 40.0% | |
| TTTCTACCAGAGGGAATCAT+GGG | - | Chr6:69611708-69611727 | MsG0680033619.01.T01:intron | 40.0% | |
| ! | AGTTACAAGAGCGTATGTTG+AGG | - | Chr6:69611909-69611928 | MsG0680033619.01.T01:CDS | 40.0% |
| ! | GATGGTTTGTCGTCAGATAT+TGG | - | Chr6:69611986-69612005 | MsG0680033619.01.T01:CDS | 40.0% |
| ! | GTATGATTTGAGGTCGATTG+AGG | - | Chr6:69611521-69611540 | MsG0680033619.01.T01:CDS | 40.0% |
| !! | CTTGTGAAGATTTTAAGGGG+TGG | - | Chr6:69611935-69611954 | MsG0680033619.01.T01:CDS | 40.0% |
| !! | TCACACTGTTTTTCTTAGGG+AGG | - | Chr6:69611736-69611755 | MsG0680033619.01.T01:intron | 40.0% |
| CAAGGTGAGGATGAAGAAGA+TGG | - | Chr6:69611968-69611987 | MsG0680033619.01.T01:CDS | 45.0% | |
| CACCTTATGAGGATGTTTCG+GGG | - | Chr6:69611499-69611518 | MsG0680033619.01.T01:CDS | 45.0% | |
| CGCAGAAGAGAATGGAGAAA+AGG | - | Chr6:69611544-69611563 | MsG0680033619.01.T01:CDS | 45.0% | |
| CTTAGGGAGGCTTATAGGAA+AGG | - | Chr6:69611749-69611768 | MsG0680033619.01.T01:intron | 45.0% | |
| GAACTTGCCGAAGAAGTTGA+AGG | - | Chr6:69611653-69611672 | MsG0680033619.01.T01:intron | 45.0% | |
| GGCTTATAGGAAAGGTGAGT+TGG | - | Chr6:69611757-69611776 | MsG0680033619.01.T01:intron | 45.0% | |
| GTTTCTACCAGAGGGAATCA+TGG | - | Chr6:69611707-69611726 | MsG0680033619.01.T01:intron | 45.0% | |
| TACCCCGAAACATCCTCATA+AGG | + | Chr6:69611504-69611523 | None:intergenic | 45.0% | |
| ! | AGGAGTTTTGCTTCAGCATC+AGG | - | Chr6:69611673-69611692 | MsG0680033619.01.T01:intron | 45.0% |
| ! | GAGTTTTGCTTCAGCATCAG+GGG | - | Chr6:69611675-69611694 | MsG0680033619.01.T01:intron | 45.0% |
| ! | GGAGTTTTGCTTCAGCATCA+GGG | - | Chr6:69611674-69611693 | MsG0680033619.01.T01:intron | 45.0% |
| ! | TGGAGAGGATTGCGCATTTT+CGG | - | Chr6:69611622-69611641 | MsG0680033619.01.T01:intron | 45.0% |
| !! | AGCTAGGGTTGATAGGGAAT+TGG | - | Chr6:69611846-69611865 | MsG0680033619.01.T01:CDS | 45.0% |
| !! | AGGGTTGATAGGGAATTGGT+TGG | - | Chr6:69611850-69611869 | MsG0680033619.01.T01:CDS | 45.0% |
| !! | GGGTTGATAGGGAATTGGTT+GGG | - | Chr6:69611851-69611870 | MsG0680033619.01.T01:CDS | 45.0% |
| !! | TTTTAAGGGGTGGATCGTGT+AGG | - | Chr6:69611945-69611964 | MsG0680033619.01.T01:CDS | 45.0% |
| AAGCTTCCCATGATTCCCTC+TGG | + | Chr6:69611717-69611736 | None:intergenic | 50.0% | |
| GATTGAGGCGCAGAAGAGAA+TGG | - | Chr6:69611536-69611555 | MsG0680033619.01.T01:CDS | 50.0% | |
| GGGAATTGGTTGGGTATAGG+AGG | - | Chr6:69611860-69611879 | MsG0680033619.01.T01:CDS | 50.0% | |
| ! | AGCTTGTGTTGTTGAGTCCC+CGG | - | Chr6:69611821-69611840 | MsG0680033619.01.T01:CDS | 50.0% |
| ! | CCCTATCAACCCTAGCTTTC+CGG | + | Chr6:69611843-69611862 | None:intergenic | 50.0% |
| ! | CCTATCAACCCTAGCTTTCC+GGG | + | Chr6:69611842-69611861 | None:intergenic | 50.0% |
| ! | CTATCAACCCTAGCTTTCCG+GGG | + | Chr6:69611841-69611860 | None:intergenic | 50.0% |
| ! | GAGGATTGCGCATTTTCGGA+TGG | - | Chr6:69611626-69611645 | MsG0680033619.01.T01:intron | 50.0% |
| !! | CCGGAAAGCTAGGGTTGATA+GGG | - | Chr6:69611840-69611859 | MsG0680033619.01.T01:CDS | 50.0% |
| GTTGAGTCCCCGGAAAGCTA+GGG | - | Chr6:69611831-69611850 | MsG0680033619.01.T01:CDS | 55.0% | |
| TGGATCGTGTAGGCAAGGTG+AGG | - | Chr6:69611955-69611974 | MsG0680033619.01.T01:CDS | 55.0% | |
| TGTTGAGTCCCCGGAAAGCT+AGG | - | Chr6:69611830-69611849 | MsG0680033619.01.T01:CDS | 55.0% | |
| ! | CCCGGAAAGCTAGGGTTGAT+AGG | - | Chr6:69611839-69611858 | MsG0680033619.01.T01:CDS | 55.0% |
| AGGGGTGGATCGTGTAGGCA+AGG | - | Chr6:69611950-69611969 | MsG0680033619.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 69611468 | 69612081 | 69611468 | ID=MsG0680033619.01;Name=MsG0680033619.01 |
| Chr6 | mRNA | 69611468 | 69612081 | 69611468 | ID=MsG0680033619.01.T01;Parent=MsG0680033619.01;Name=MsG0680033619.01.T01;_AED=0.38;_eAED=0.38;_QI=0|0|0|1|1|1|2|0|141 |
| Chr6 | exon | 69611771 | 69612081 | 69611771 | ID=MsG0680033619.01.T01:exon:2230;Parent=MsG0680033619.01.T01 |
| Chr6 | exon | 69611468 | 69611582 | 69611468 | ID=MsG0680033619.01.T01:exon:2231;Parent=MsG0680033619.01.T01 |
| Chr6 | CDS | 69611771 | 69612081 | 69611771 | ID=MsG0680033619.01.T01:cds;Parent=MsG0680033619.01.T01 |
| Chr6 | CDS | 69611468 | 69611582 | 69611468 | ID=MsG0680033619.01.T01:cds;Parent=MsG0680033619.01.T01 |
| Gene Sequence |
| Protein sequence |
