AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040872.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040872.01.T01 MTR_7g100230 92.105 152 12 0 12 163 12 163 1.42e-91 263
MsG0780040872.01.T01 MTR_7g100200 61.538 156 57 2 8 163 7 159 1.14e-53 167
MsG0780040872.01.T01 MTR_7g100170 49.057 53 26 1 65 116 75 127 1.20e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040872.01.T01 AT3G58090 51.754 114 44 2 9 120 8 112 6.73e-33 118

Find 53 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCGAACCACATAGGAATT+TGG 0.220618 7:+83717282 MsG0780040872.01.T01:CDS
AAGAAATCTATTCCTAAATC+TGG 0.252000 7:+83717529 MsG0780040872.01.T01:CDS
GCACTACTCTTCCCAGATTT+AGG 0.253924 7:-83717541 None:intergenic
CTGCTACCGGATACTATTTC+AGG 0.274069 7:-83717220 None:intergenic
TGGGAGAATGGTTTGTGGGG+AGG 0.323895 7:+83717308 MsG0780040872.01.T01:CDS
AGAGGGCAAAGAAGAAAATA+AGG 0.333305 7:+83717126 MsG0780040872.01.T01:CDS
GGGAGAATGGTTTGTGGGGA+GGG 0.334434 7:+83717309 MsG0780040872.01.T01:CDS
ATCGAACCACATAGGAATTT+GGG 0.364456 7:+83717283 MsG0780040872.01.T01:CDS
GGCTTGGGAGAATGGTTTGT+GGG 0.370784 7:+83717304 MsG0780040872.01.T01:CDS
ACCGAGTATGACAAAGAATC+TGG 0.379264 7:+83717376 MsG0780040872.01.T01:CDS
GGAATTTGGGCTTGGGAGAA+TGG 0.402637 7:+83717296 MsG0780040872.01.T01:CDS
ACCACATAGGAATTTGGGCT+TGG 0.411883 7:+83717288 MsG0780040872.01.T01:CDS
GGGCTTGGGAGAATGGTTTG+TGG 0.416018 7:+83717303 MsG0780040872.01.T01:CDS
CATTGGCGCAGAGGGTTGTT+CGG 0.423791 7:+83717500 MsG0780040872.01.T01:CDS
ATCAAAACAAAGAGAACAAA+AGG 0.438164 7:+83717583 MsG0780040872.01.T01:CDS
GTGATTCTGAAGATCTTGAT+TGG 0.441563 7:+83717425 MsG0780040872.01.T01:CDS
GACGGCGCTGCTGCTGCTAC+CGG 0.450572 7:-83717233 None:intergenic
TCTTGATTGGCCGGAGTTGA+AGG 0.457279 7:+83717438 MsG0780040872.01.T01:CDS
GAAGTTCTCAGTCCTTTAGA+TGG 0.458489 7:+83717460 MsG0780040872.01.T01:CDS
ATTACAGTCGCAGGCTCTCC+AGG 0.465049 7:-83717187 None:intergenic
ATCTAATTCTTCTGCGGCAA+GGG 0.467917 7:+83717153 MsG0780040872.01.T01:CDS
CCCAGATTCTTTGTCATACT+CGG 0.473382 7:-83717377 None:intergenic
AGCATTGCTGCTGGAAGGGA+CGG 0.473470 7:-83717251 None:intergenic
TTCTGAAGATCTTGATTGGC+CGG 0.482353 7:+83717429 MsG0780040872.01.T01:CDS
GCTTGGGAGAATGGTTTGTG+GGG 0.486598 7:+83717305 MsG0780040872.01.T01:CDS
CCACATAGGAATTTGGGCTT+GGG 0.489077 7:+83717289 MsG0780040872.01.T01:CDS
AGTGAAATTCCACCATCTAA+AGG 0.490197 7:-83717472 None:intergenic
AGAAATCTATTCCTAAATCT+GGG 0.513142 7:+83717530 MsG0780040872.01.T01:CDS
GATACTCAAAGCATTGCTGC+TGG 0.527679 7:-83717260 None:intergenic
CTCAAAGCATTGCTGCTGGA+AGG 0.529613 7:-83717256 None:intergenic
GTTTATGGGACGTTATTACT+CGG 0.537141 7:+83717345 MsG0780040872.01.T01:CDS
ACTCAAGACATTGGCGCAGA+GGG 0.544285 7:+83717492 MsG0780040872.01.T01:CDS
CTGAGAACTTCCTTCAACTC+CGG 0.550027 7:-83717448 None:intergenic
TCAAAGCATTGCTGCTGGAA+GGG 0.554064 7:-83717255 None:intergenic
GTTTCTGTTTGAAATGGAAG+AGG 0.555062 7:+83717108 None:intergenic
TGGAATTTCACTCAAGACAT+TGG 0.557096 7:+83717483 MsG0780040872.01.T01:CDS
TTTCTGTTTGAAATGGAAGA+GGG 0.564578 7:+83717109 None:intergenic
CCCAAGCCCAAATTCCTATG+TGG 0.565828 7:-83717289 None:intergenic
GGGCAAAGAAGAAAATAAGG+AGG 0.566148 7:+83717129 MsG0780040872.01.T01:CDS
CACCGTCTTCATACTCAACA+TGG 0.568627 7:-83717405 None:intergenic
CACTCAAGACATTGGCGCAG+AGG 0.573004 7:+83717491 MsG0780040872.01.T01:CDS
TTTATGGGACGTTATTACTC+GGG 0.573677 7:+83717346 MsG0780040872.01.T01:CDS
GTTCTCAGTCCTTTAGATGG+TGG 0.581755 7:+83717463 MsG0780040872.01.T01:CDS
GAGCCTGCGACTGTAATTGA+CGG 0.584299 7:+83717193 MsG0780040872.01.T01:CDS
GATCTAATTCTTCTGCGGCA+AGG 0.585721 7:+83717152 MsG0780040872.01.T01:CDS
GGAGTGCCTGAAATAGTATC+CGG 0.596939 7:+83717214 MsG0780040872.01.T01:CDS
CCGAGTATGACAAAGAATCT+GGG 0.597649 7:+83717377 MsG0780040872.01.T01:CDS
ACTCCGTCAATTACAGTCGC+AGG 0.599135 7:-83717196 None:intergenic
TGAGTATCATCGAACCACAT+AGG 0.617574 7:+83717275 MsG0780040872.01.T01:CDS
GGCAAAGAAGAAAATAAGGA+GGG 0.632888 7:+83717130 MsG0780040872.01.T01:CDS
GGAGGGATCTAATTCTTCTG+CGG 0.660755 7:+83717147 MsG0780040872.01.T01:CDS
TACCATGTTGAGTATGAAGA+CGG 0.707224 7:+83717403 MsG0780040872.01.T01:CDS
AGTATGACAAAGAATCTGGG+TGG 0.768571 7:+83717380 MsG0780040872.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATGTTCAAAAATTGTTTAT+GGG + Chr7:83717331-83717350 MsG0780040872.01.T01:CDS 20.0%
! AAGAAATCTATTCCTAAATC+TGG + Chr7:83717529-83717548 MsG0780040872.01.T01:CDS 25.0%
! AGAAATCTATTCCTAAATCT+GGG + Chr7:83717530-83717549 MsG0780040872.01.T01:CDS 25.0%
! ATCAAAACAAAGAGAACAAA+AGG + Chr7:83717583-83717602 MsG0780040872.01.T01:CDS 25.0%
! GGATGTTCAAAAATTGTTTA+TGG + Chr7:83717330-83717349 MsG0780040872.01.T01:CDS 25.0%
!!! GTTCTCTTTGTTTTGATTTG+AGG - Chr7:83717580-83717599 None:intergenic 30.0%
AGAGGGCAAAGAAGAAAATA+AGG + Chr7:83717126-83717145 MsG0780040872.01.T01:CDS 35.0%
AGTGAAATTCCACCATCTAA+AGG - Chr7:83717475-83717494 None:intergenic 35.0%
ATCGAACCACATAGGAATTT+GGG + Chr7:83717283-83717302 MsG0780040872.01.T01:CDS 35.0%
GGCAAAGAAGAAAATAAGGA+GGG + Chr7:83717130-83717149 MsG0780040872.01.T01:CDS 35.0%
GTTTATGGGACGTTATTACT+CGG + Chr7:83717345-83717364 MsG0780040872.01.T01:CDS 35.0%
TACCATGTTGAGTATGAAGA+CGG + Chr7:83717403-83717422 MsG0780040872.01.T01:CDS 35.0%
TGGAATTTCACTCAAGACAT+TGG + Chr7:83717483-83717502 MsG0780040872.01.T01:CDS 35.0%
TTTATGGGACGTTATTACTC+GGG + Chr7:83717346-83717365 MsG0780040872.01.T01:CDS 35.0%
! GTGATTCTGAAGATCTTGAT+TGG + Chr7:83717425-83717444 MsG0780040872.01.T01:CDS 35.0%
ACCGAGTATGACAAAGAATC+TGG + Chr7:83717376-83717395 MsG0780040872.01.T01:CDS 40.0%
AGTATGACAAAGAATCTGGG+TGG + Chr7:83717380-83717399 MsG0780040872.01.T01:CDS 40.0%
ATCTAATTCTTCTGCGGCAA+GGG + Chr7:83717153-83717172 MsG0780040872.01.T01:CDS 40.0%
CATCGAACCACATAGGAATT+TGG + Chr7:83717282-83717301 MsG0780040872.01.T01:CDS 40.0%
CCCAGATTCTTTGTCATACT+CGG - Chr7:83717380-83717399 None:intergenic 40.0%
CCGAGTATGACAAAGAATCT+GGG + Chr7:83717377-83717396 MsG0780040872.01.T01:CDS 40.0%
GAAGTTCTCAGTCCTTTAGA+TGG + Chr7:83717460-83717479 MsG0780040872.01.T01:CDS 40.0%
GGGCAAAGAAGAAAATAAGG+AGG + Chr7:83717129-83717148 MsG0780040872.01.T01:CDS 40.0%
TGAGTATCATCGAACCACAT+AGG + Chr7:83717275-83717294 MsG0780040872.01.T01:CDS 40.0%
! GCAAGGGTTTATGATTTTCC+TGG + Chr7:83717169-83717188 MsG0780040872.01.T01:CDS 40.0%
! TTCTGAAGATCTTGATTGGC+CGG + Chr7:83717429-83717448 MsG0780040872.01.T01:CDS 40.0%
ACCACATAGGAATTTGGGCT+TGG + Chr7:83717288-83717307 MsG0780040872.01.T01:CDS 45.0%
CACCGTCTTCATACTCAACA+TGG - Chr7:83717408-83717427 None:intergenic 45.0%
CCACATAGGAATTTGGGCTT+GGG + Chr7:83717289-83717308 MsG0780040872.01.T01:CDS 45.0%
CTGAGAACTTCCTTCAACTC+CGG - Chr7:83717451-83717470 None:intergenic 45.0%
CTGCTACCGGATACTATTTC+AGG - Chr7:83717223-83717242 None:intergenic 45.0%
GATCTAATTCTTCTGCGGCA+AGG + Chr7:83717152-83717171 MsG0780040872.01.T01:CDS 45.0%
GCACTACTCTTCCCAGATTT+AGG - Chr7:83717544-83717563 None:intergenic 45.0%
GGAGGGATCTAATTCTTCTG+CGG + Chr7:83717147-83717166 MsG0780040872.01.T01:CDS 45.0%
GTTCTCAGTCCTTTAGATGG+TGG + Chr7:83717463-83717482 MsG0780040872.01.T01:CDS 45.0%
! GGAGTGCCTGAAATAGTATC+CGG + Chr7:83717214-83717233 MsG0780040872.01.T01:CDS 45.0%
!! GATACTCAAAGCATTGCTGC+TGG - Chr7:83717263-83717282 None:intergenic 45.0%
!! TCAAAGCATTGCTGCTGGAA+GGG - Chr7:83717258-83717277 None:intergenic 45.0%
ACTCAAGACATTGGCGCAGA+GGG + Chr7:83717492-83717511 MsG0780040872.01.T01:CDS 50.0%
ACTCCGTCAATTACAGTCGC+AGG - Chr7:83717199-83717218 None:intergenic 50.0%
CCCAAGCCCAAATTCCTATG+TGG - Chr7:83717292-83717311 None:intergenic 50.0%
GAGCCTGCGACTGTAATTGA+CGG + Chr7:83717193-83717212 MsG0780040872.01.T01:CDS 50.0%
GGAATTTGGGCTTGGGAGAA+TGG + Chr7:83717296-83717315 MsG0780040872.01.T01:CDS 50.0%
! GCTTGGGAGAATGGTTTGTG+GGG + Chr7:83717305-83717324 MsG0780040872.01.T01:CDS 50.0%
! GGCTTGGGAGAATGGTTTGT+GGG + Chr7:83717304-83717323 MsG0780040872.01.T01:CDS 50.0%
! TCTTGATTGGCCGGAGTTGA+AGG + Chr7:83717438-83717457 MsG0780040872.01.T01:CDS 50.0%
!! CTCAAAGCATTGCTGCTGGA+AGG - Chr7:83717259-83717278 None:intergenic 50.0%
AGCATTGCTGCTGGAAGGGA+CGG - Chr7:83717254-83717273 None:intergenic 55.0%
ATTACAGTCGCAGGCTCTCC+AGG - Chr7:83717190-83717209 None:intergenic 55.0%
CACTCAAGACATTGGCGCAG+AGG + Chr7:83717491-83717510 MsG0780040872.01.T01:CDS 55.0%
CATTGGCGCAGAGGGTTGTT+CGG + Chr7:83717500-83717519 MsG0780040872.01.T01:CDS 55.0%
GGGCTTGGGAGAATGGTTTG+TGG + Chr7:83717303-83717322 MsG0780040872.01.T01:CDS 55.0%
! GGGAGAATGGTTTGTGGGGA+GGG + Chr7:83717309-83717328 MsG0780040872.01.T01:CDS 55.0%
! TGGGAGAATGGTTTGTGGGG+AGG + Chr7:83717308-83717327 MsG0780040872.01.T01:CDS 55.0%
GACGGCGCTGCTGCTGCTAC+CGG - Chr7:83717236-83717255 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 83717121 83717612 83717121 ID=MsG0780040872.01;Name=MsG0780040872.01
Chr7 mRNA 83717121 83717612 83717121 ID=MsG0780040872.01.T01;Parent=MsG0780040872.01;Name=MsG0780040872.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|163
Chr7 exon 83717121 83717612 83717121 ID=MsG0780040872.01.T01:exon:19882;Parent=MsG0780040872.01.T01
Chr7 CDS 83717121 83717612 83717121 ID=MsG0780040872.01.T01:cds;Parent=MsG0780040872.01.T01
Gene Sequence

>MsG0780040872.01.T01

ATGGAAGAGGGCAAAGAAGAAAATAAGGAGGGATCTAATTCTTCTGCGGCAAGGGTTTATGATTTTCCTGGAGAGCCTGCGACTGTAATTGACGGAGTGCCTGAAATAGTATCCGGTAGCAGCAGCAGCGCCGTCCCTTCCAGCAGCAATGCTTTGAGTATCATCGAACCACATAGGAATTTGGGCTTGGGAGAATGGTTTGTGGGGAGGGATGTTCAAAAATTGTTTATGGGACGTTATTACTCGGGTAGAGTCACCGAGTATGACAAAGAATCTGGGTGGTACCATGTTGAGTATGAAGACGGTGATTCTGAAGATCTTGATTGGCCGGAGTTGAAGGAAGTTCTCAGTCCTTTAGATGGTGGAATTTCACTCAAGACATTGGCGCAGAGGGTTGTTCGGAATAGTAAGAAATCTATTCCTAAATCTGGGAAGAGTAGTGCTCGTTCGCAAAATCCTCAAATCAAAACAAAGAGAACAAAAGGAAATTAG

Protein sequence

>MsG0780040872.01.T01

MEEGKEENKEGSNSSAARVYDFPGEPATVIDGVPEIVSGSSSSAVPSSSNALSIIEPHRNLGLGEWFVGRDVQKLFMGRYYSGRVTEYDKESGWYHVEYEDGDSEDLDWPELKEVLSPLDGGISLKTLAQRVVRNSKKSIPKSGKSSARSQNPQIKTKRTKGN*