AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004600.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004600.01.T02 MTR_1g083380 96.429 84 3 0 1 84 1 84 6.44e-54 165
MsG0180004600.01.T02 MTR_1g083350 96.429 84 3 0 1 84 1 84 6.51e-54 165
MsG0180004600.01.T02 MTR_1g083340 87.234 94 2 1 1 84 1 94 1.56e-51 159
MsG0180004600.01.T02 MTR_2g436620 84.524 84 12 1 1 84 1 83 1.20e-44 141
MsG0180004600.01.T02 MTR_6g071090 79.762 84 16 1 1 84 1 83 6.97e-40 129
MsG0180004600.01.T02 MTR_2g436620 82.759 58 9 1 27 84 1 57 3.02e-27 97.1
MsG0180004600.01.T01 MTR_1g083380 96.429 84 3 0 1 84 1 84 6.44e-54 165
MsG0180004600.01.T01 MTR_1g083350 96.429 84 3 0 1 84 1 84 6.51e-54 165
MsG0180004600.01.T01 MTR_1g083340 87.234 94 2 1 1 84 1 94 1.56e-51 159
MsG0180004600.01.T01 MTR_2g436620 84.524 84 12 1 1 84 1 83 1.20e-44 141
MsG0180004600.01.T01 MTR_6g071090 79.762 84 16 1 1 84 1 83 6.97e-40 129
MsG0180004600.01.T01 MTR_2g436620 82.759 58 9 1 27 84 1 57 3.02e-27 97.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004600.01.T02 AT3G16640 84.524 84 13 0 1 84 1 84 8.80e-46 144
MsG0180004600.01.T02 AT3G05540 83.333 84 13 1 1 84 1 83 9.67e-38 124
MsG0180004600.01.T01 AT3G16640 84.524 84 13 0 1 84 1 84 8.80e-46 144
MsG0180004600.01.T01 AT3G05540 83.333 84 13 1 1 84 1 83 9.67e-38 124

Find 10 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCAACCATGCTTGTTTACC+AGG 0.349778 1:-80205536 MsG0180004600.01.T01:exon
GAAAGAGTTCAAGTGATTGT+AGG 0.357231 1:+80205661 None:intergenic
GTTTACCAGGATCTTCTTAC+GGG 0.390880 1:-80205523 MsG0180004600.01.T01:intron
TGTTTACCAGGATCTTCTTA+CGG 0.395300 1:-80205524 MsG0180004600.01.T01:CDS
AATAAGAGAGAAGCAAAAGA+AGG 0.503108 1:+80205571 None:intergenic
AAAGTGAGATTGAGCAATAG+AGG 0.537193 1:+80205612 None:intergenic
GTTTACCCGTAAGAAGATCC+TGG 0.562606 1:+80205518 None:intergenic
GTGAGATTGAGCAATAGAGG+TGG 0.598021 1:+80205615 None:intergenic
CTGGTAAACAAGCATGGTTG+CGG 0.598178 1:+80205537 None:intergenic
AAGATCCTGGTAAACAAGCA+TGG 0.602746 1:+80205531 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTATGATCATATATT+TGG + Chr1:80204965-80204984 None:intergenic 10.0%
!!! ATTAAAATTTTGGATAATAA+TGG + Chr1:80204534-80204553 None:intergenic 10.0%
!!! TTTAATTTAATAGAATTTGT+TGG - Chr1:80204548-80204567 MsG0180004600.01.T01:CDS 10.0%
!! AAAACAAAAATTCATTCAAA+TGG - Chr1:80204764-80204783 MsG0180004600.01.T01:intron 15.0%
!! ATTATAAAATTTCAGTAGTA+TGG - Chr1:80205691-80205710 MsG0180004600.01.T02:five_prime_UTR 15.0%
!!! TTATATATTAACCTTTTGTA+AGG - Chr1:80204334-80204353 MsG0180004600.01.T01:three_prime_UTR 15.0%
!!! TTTTTTTTTTTTTTGACACA+AGG - Chr1:80204386-80204405 MsG0180004600.01.T01:three_prime_UTR 15.0%
!! AAAAGTAAAAGAAACCTAAT+TGG + Chr1:80204129-80204148 None:intergenic 20.0%
!! AAAGTAAAAGAAACCTAATT+GGG + Chr1:80204128-80204147 None:intergenic 20.0%
!! TAATCTTAAATATTAGTAGC+AGG + Chr1:80204715-80204734 None:intergenic 20.0%
!! TTATCATTCATCGTAATATT+TGG + Chr1:80204207-80204226 None:intergenic 20.0%
!!! ACAAGGTTAAAATACTTTTT+TGG - Chr1:80204403-80204422 MsG0180004600.01.T01:intron 20.0%
!!! ATTAAGTTTTCTCTTTTTTG+TGG - Chr1:80205343-80205362 MsG0180004600.01.T01:intron 20.0%
!!! ATTATCTTTCATCTCTTTTT+TGG - Chr1:80205316-80205335 MsG0180004600.01.T01:intron 20.0%
!!! TTTTTCATAAACTTCACTTT+TGG - Chr1:80204282-80204301 MsG0180004600.01.T01:intron 20.0%
! AAAATCCACAATCAAGTATT+AGG + Chr1:80204675-80204694 None:intergenic 25.0%
! AAATATTACTTAGCAGTTTG+TGG + Chr1:80204626-80204645 None:intergenic 25.0%
! AAATTCATTCACTAAACACA+GGG + Chr1:80204239-80204258 None:intergenic 25.0%
! AAATTTCAGTAGTATGGTTA+TGG - Chr1:80205697-80205716 MsG0180004600.01.T02:five_prime_UTR 25.0%
! AATCTTAACAACCTTACAAA+AGG + Chr1:80204348-80204367 None:intergenic 25.0%
! ACACATTTAGTTATCATGTT+AGG - Chr1:80205518-80205537 MsG0180004600.01.T01:intron 25.0%
! ACTATTATAGATCAAACTTG+TGG - Chr1:80204457-80204476 MsG0180004600.01.T01:CDS 25.0%
! CAAAATCCTATGAAAAACAA+TGG + Chr1:80204154-80204173 None:intergenic 25.0%
! GAGTTTACTATTCTCTAATT+AGG - Chr1:80205616-80205635 MsG0180004600.01.T01:intron 25.0%
! GATGAAATACATACAATTGT+AGG + Chr1:80205296-80205315 None:intergenic 25.0%
! TGGAAAACCTTAAATTTCTT+GGG - Chr1:80204423-80204442 MsG0180004600.01.T01:CDS 25.0%
! TTGGAAAACCTTAAATTTCT+TGG - Chr1:80204422-80204441 MsG0180004600.01.T01:CDS 25.0%
! TTTACTATTCTCTAATTAGG+AGG - Chr1:80205619-80205638 MsG0180004600.01.T01:intron 25.0%
!! GATAATAATGGAAAACCTAA+AGG + Chr1:80204522-80204541 None:intergenic 25.0%
!!! TTTTCTCCATTGTTTTTCAT+AGG - Chr1:80204145-80204164 MsG0180004600.01.T01:three_prime_UTR 25.0%
AAATTCAGAATGGAATGTTG+TGG - Chr1:80204863-80204882 MsG0180004600.01.T01:intron 30.0%
AATAAGAGAGAAGCAAAAGA+AGG + Chr1:80204018-80204037 None:intergenic 30.0%
AATTCAGAATGGAATGTTGT+GGG - Chr1:80204864-80204883 MsG0180004600.01.T01:intron 30.0%
ACAAAAAACTCAAAGATGCA+AGG + Chr1:80204093-80204112 None:intergenic 30.0%
AGTAACAACTTATCAAAAGC+TGG + Chr1:80205160-80205179 None:intergenic 30.0%
ATTCTTAATCGATAGTACTC+TGG - Chr1:80205559-80205578 MsG0180004600.01.T01:five_prime_UTR 30.0%
CACATCACACAATAAACTTT+AGG + Chr1:80204991-80205010 None:intergenic 30.0%
GAAATTCATTCACTAAACAC+AGG + Chr1:80204240-80204259 None:intergenic 30.0%
GATCTATAATAGTGTGTGTT+TGG + Chr1:80204450-80204469 None:intergenic 30.0%
! CTTTGTTGATGAATTTGTCT+AGG - Chr1:80204805-80204824 MsG0180004600.01.T01:intron 30.0%
! TTTTACTTTACCACCCAATT+AGG - Chr1:80204112-80204131 MsG0180004600.01.T01:three_prime_UTR 30.0%
!! AATTGTAGGTCTTTGAGTTT+TGG + Chr1:80205282-80205301 None:intergenic 30.0%
!! AGTTTTATGTTTGTGTTCTG+TGG - Chr1:80204491-80204510 MsG0180004600.01.T01:CDS 30.0%
!! ATTGTAGGTCTTTGAGTTTT+GGG + Chr1:80205281-80205300 None:intergenic 30.0%
!!! ATCAATGTTGCAGTTTTGTT+GGG - Chr1:80205387-80205406 MsG0180004600.01.T01:intron 30.0%
AAAGTGAGATTGAGCAATAG+AGG + Chr1:80203977-80203996 None:intergenic 35.0%
ATATTGTTCTCAGAGCAACA+TGG - Chr1:80205664-80205683 MsG0180004600.01.T02:five_prime_UTR 35.0%
CATTCTGAATTTCCTTGTAG+AGG + Chr1:80204855-80204874 None:intergenic 35.0%
CCACAATCAAGTATTAGGTT+AGG + Chr1:80204670-80204689 None:intergenic 35.0%
CCTAACCTAATACTTGATTG+TGG - Chr1:80204667-80204686 MsG0180004600.01.T01:intron 35.0%
CTCTACAAGGAAATTCAGAA+TGG - Chr1:80204853-80204872 MsG0180004600.01.T01:intron 35.0%
GAAAGAGTTCAAGTGATTGT+AGG + Chr1:80203928-80203947 None:intergenic 35.0%
GTAAAAGAAACCTAATTGGG+TGG + Chr1:80204125-80204144 None:intergenic 35.0%
GTGATGTGATTATTTGCAGT+GGG - Chr1:80205003-80205022 MsG0180004600.01.T01:intron 35.0%
GTTAGGCTAATCGTGTTTAA+GGG + Chr1:80204653-80204672 None:intergenic 35.0%
GTTTGGTCCCAAGAAATTTA+AGG + Chr1:80204433-80204452 None:intergenic 35.0%
TAGGCAAAGTAGAGAAAAGT+TGG + Chr1:80205473-80205492 None:intergenic 35.0%
TGTGATGTGATTATTTGCAG+TGG - Chr1:80205002-80205021 MsG0180004600.01.T01:intron 35.0%
TGTTCTGTGGTTTAACCTTT+AGG - Chr1:80204504-80204523 MsG0180004600.01.T01:CDS 35.0%
TGTTTACCAGGATCTTCTTA+CGG - Chr1:80204062-80204081 MsG0180004600.01.T01:three_prime_UTR 35.0%
TTGACATTGTTGACACTTTC+AGG - Chr1:80205122-80205141 MsG0180004600.01.T01:intron 35.0%
! ACTTTTCTCTACTTTGCCTA+TGG - Chr1:80205473-80205492 MsG0180004600.01.T01:intron 35.0%
! CGATAGTACTCTGGTAATTT+TGG - Chr1:80205568-80205587 MsG0180004600.01.T01:five_prime_UTR 35.0%
!! CATCAATGTTGCAGTTTTGT+TGG - Chr1:80205386-80205405 MsG0180004600.01.T01:intron 35.0%
!! TTGAACAACTCTTGCTTTTC+TGG + Chr1:80205225-80205244 None:intergenic 35.0%
!!! TCAATGTTGCAGTTTTGTTG+GGG - Chr1:80205388-80205407 MsG0180004600.01.T01:intron 35.0%
AAGATCCTGGTAAACAAGCA+TGG + Chr1:80204058-80204077 None:intergenic 40.0%
AGTTGTTGTGTTTGTGACAG+AGG - Chr1:80204582-80204601 MsG0180004600.01.T01:intron 40.0%
CCTTGTAGTAAGCAAACACA+AGG + Chr1:80205436-80205455 None:intergenic 40.0%
CCTTGTGTTTGCTTACTACA+AGG - Chr1:80205433-80205452 MsG0180004600.01.T01:intron 40.0%
CTTGATTTCCTTCAAGCCAT+AGG + Chr1:80205492-80205511 None:intergenic 40.0%
GGTTAGGCTAATCGTGTTTA+AGG + Chr1:80204654-80204673 None:intergenic 40.0%
GTAGAGAAAAGTTGGATCAG+TGG + Chr1:80205465-80205484 None:intergenic 40.0%
GTGTTTGCTTACTACAAGGA+TGG - Chr1:80205437-80205456 MsG0180004600.01.T01:intron 40.0%
GTTTACCAGGATCTTCTTAC+GGG - Chr1:80204063-80204082 MsG0180004600.01.T01:three_prime_UTR 40.0%
!! TATTTGCAGTGGGTTGTTCA+AGG - Chr1:80205013-80205032 MsG0180004600.01.T01:intron 40.0%
CTACTTTGCCTATGGCTTGA+AGG - Chr1:80205481-80205500 MsG0180004600.01.T01:intron 45.0%
CTCTGACTCATTCCTCTACA+AGG - Chr1:80204840-80204859 MsG0180004600.01.T01:intron 45.0%
CTGGTAAACAAGCATGGTTG+CGG + Chr1:80204052-80204071 None:intergenic 45.0%
GAATGGAATGTTGTGGGAAG+TGG - Chr1:80204870-80204889 MsG0180004600.01.T01:intron 45.0%
GGAGCAGTGGATGTGAATAT+TGG - Chr1:80205034-80205053 MsG0180004600.01.T01:intron 45.0%
GTGAGATTGAGCAATAGAGG+TGG + Chr1:80203974-80203993 None:intergenic 45.0%
GTTTACCCGTAAGAAGATCC+TGG + Chr1:80204071-80204090 None:intergenic 45.0%
TGTTGACACTTTCAGGCTTC+AGG - Chr1:80205129-80205148 MsG0180004600.01.T01:intron 45.0%
!!! GGTCTTTGAGTTTTGGGAGA+AGG + Chr1:80205275-80205294 None:intergenic 45.0%
!!! ATTCTATTAAATTAAAATTT+TGG + Chr1:80204544-80204563 None:intergenic 5.0%
CGCAACCATGCTTGTTTACC+AGG - Chr1:80204050-80204069 MsG0180004600.01.T01:three_prime_UTR 50.0%
GGAATGTTGTGGGAAGTGGA+AGG - Chr1:80204874-80204893 MsG0180004600.01.T01:intron 50.0%
GGGAAGTGGAAGGAAAGGTT+AGG - Chr1:80204884-80204903 MsG0180004600.01.T01:intron 50.0%
GTTGTGGGAAGTGGAAGGAA+AGG - Chr1:80204879-80204898 MsG0180004600.01.T01:intron 50.0%
! GGGGAGAGTATGCATGATGA+TGG - Chr1:80205407-80205426 MsG0180004600.01.T01:intron 50.0%
GGTGCTAACCCTTCTGCTGA+GGG - Chr1:80205055-80205074 MsG0180004600.01.T01:intron 55.0%
! GTGGGTTGTTCAAGGAGCAG+TGG - Chr1:80205021-80205040 MsG0180004600.01.T01:intron 55.0%
! TGGTGCTAACCCTTCTGCTG+AGG - Chr1:80205054-80205073 MsG0180004600.01.T01:intron 55.0%
CGTCACCACCCTCAGCAGAA+GGG + Chr1:80205066-80205085 None:intergenic 60.0%
GCTAACCCTTCTGCTGAGGG+TGG - Chr1:80205058-80205077 MsG0180004600.01.T01:intron 60.0%
TCGTCACCACCCTCAGCAGA+AGG + Chr1:80205067-80205086 None:intergenic 60.0%
! GAGGGTGGTGACGAGGATGA+CGG - Chr1:80205073-80205092 MsG0180004600.01.T01:intron 60.0%
! TTCTGCTGAGGGTGGTGACG+AGG - Chr1:80205066-80205085 MsG0180004600.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 80203885 80205723 80203885 ID=MsG0180004600.01;Name=MsG0180004600.01
Chr1 mRNA 80203885 80205669 80203885 ID=MsG0180004600.01.T01;Parent=MsG0180004600.01;Name=MsG0180004600.01.T01;_AED=0.49;_eAED=0.49;_QI=96|1|1|1|0.75|0.4|5|439|88
Chr1 exon 80205524 80205614 80205524 ID=MsG0180004600.01.T01:exon:45856;Parent=MsG0180004600.01.T01
Chr1 exon 80205637 80205669 80205637 ID=MsG0180004600.01.T01:exon:45855;Parent=MsG0180004600.01.T01
Chr1 five_prime_UTR 80205637 80205669 80205637 ID=MsG0180004600.01.T01:five_prime_utr;Parent=MsG0180004600.01.T01
Chr1 five_prime_UTR 80205552 80205614 80205552 ID=MsG0180004600.01.T01:five_prime_utr;Parent=MsG0180004600.01.T01
Chr1 CDS 80205524 80205551 80205524 ID=MsG0180004600.01.T01:cds;Parent=MsG0180004600.01.T01
Chr1 CDS 80204708 80204781 80204708 ID=MsG0180004600.01.T01:cds;Parent=MsG0180004600.01.T01
Chr1 CDS 80204422 80204586 80204422 ID=MsG0180004600.01.T01:cds;Parent=MsG0180004600.01.T01
Chr1 three_prime_UTR 80204307 80204421 80204307 ID=MsG0180004600.01.T01:three_prime_utr;Parent=MsG0180004600.01.T01
Chr1 three_prime_UTR 80203885 80204208 80203885 ID=MsG0180004600.01.T01:three_prime_utr;Parent=MsG0180004600.01.T01
Chr1 mRNA 80203885 80205723 80203885 ID=MsG0180004600.01.T02;Parent=MsG0180004600.01;Name=MsG0180004600.01.T02;_AED=0.49;_eAED=0.49;_QI=172|1|1|1|1|0.75|4|439|88
Chr1 exon 80205524 80205723 80205524 ID=MsG0180004600.01.T02:exon:45860;Parent=MsG0180004600.01.T02
Chr1 five_prime_UTR 80205552 80205723 80205552 ID=MsG0180004600.01.T02:five_prime_utr;Parent=MsG0180004600.01.T02
Chr1 CDS 80205524 80205551 80205524 ID=MsG0180004600.01.T02:cds;Parent=MsG0180004600.01.T02
Chr1 CDS 80204708 80204781 80204708 ID=MsG0180004600.01.T02:cds;Parent=MsG0180004600.01.T02
Chr1 CDS 80204422 80204586 80204422 ID=MsG0180004600.01.T02:cds;Parent=MsG0180004600.01.T02
Chr1 three_prime_UTR 80204307 80204421 80204307 ID=MsG0180004600.01.T02:three_prime_utr;Parent=MsG0180004600.01.T02
Chr1 three_prime_UTR 80203885 80204208 80203885 ID=MsG0180004600.01.T02:three_prime_utr;Parent=MsG0180004600.01.T02
Gene Sequence

>MsG0180004600.01.T02

ATGCTTGTTTACCAGGATCTTCTTACGGGTGATGAGCTTCTCTCTGACTCATTCCTCTACAAGGAAATTCAGAATGGAATGTTGTGGGAAGTGGAAGGAAAGTGGGTTGTTCAAGGAGCAGTGGATGTGAATATTGGTGCTAACCCTTCTGCTGAGGGTGGTGACGAGGATGACGGTGTTGATGATCAAGCTGTCAAAGTTGTTGACATTGTTGACACTTTCAGGCTTCAGGAGCAACCAGCTTTTGATAAGTTGTTACTTATATGA

>MsG0180004600.01.T01

ATGCTTGTTTACCAGGATCTTCTTACGGGTGATGAGCTTCTCTCTGACTCATTCCTCTACAAGGAAATTCAGAATGGAATGTTGTGGGAAGTGGAAGGAAAGTGGGTTGTTCAAGGAGCAGTGGATGTGAATATTGGTGCTAACCCTTCTGCTGAGGGTGGTGACGAGGATGACGGTGTTGATGATCAAGCTGTCAAAGTTGTTGACATTGTTGACACTTTCAGGCTTCAGGAGCAACCAGCTTTTGATAAGTTGTTACTTATATGA

Protein sequence

>MsG0180004600.01.T02

MLVYQDLLTGDELLSDSFLYKEIQNGMLWEVEGKWVVQGAVDVNIGANPSAEGGDEDDGVDDQAVKVVDIVDTFRLQEQPAFDKLLLI*

>MsG0180004600.01.T01

MLVYQDLLTGDELLSDSFLYKEIQNGMLWEVEGKWVVQGAVDVNIGANPSAEGGDEDDGVDDQAVKVVDIVDTFRLQEQPAFDKLLLI*