AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020791.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020791.01.T01 MTR_4g058950 98.701 77 1 0 1 77 1 77 4.87e-50 155
MsG0480020791.01.T01 MTR_7g078440 58.108 74 17 1 1 74 1 60 7.32e-23 83.2
MsG0480020791.01.T01 MTR_5g033270 55.405 74 12 1 1 74 36 88 1.37e-18 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020791.01.T01 AT2G37200 57.143 77 28 1 1 77 2 73 1.10e-20 80.9
MsG0480020791.01.T01 AT2G28370 66.234 77 20 1 1 77 1 71 1.17e-20 80.9
MsG0480020791.01.T01 AT2G37200 57.143 77 28 1 1 77 31 102 1.80e-20 80.9

Find 22 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTTCTGTCACTGCCTTC+TGG 0.281533 4:+48389670 MsG0480020791.01.T01:CDS
GGCTGCTGCACTATCTGTTA+TGG 0.379001 4:+48389632 MsG0480020791.01.T01:CDS
GTAGTTGGAACTTCTTCAAC+TGG 0.379154 4:-48389486 None:intergenic
CAAGGCATGCCTGGTACTAT+TGG 0.395458 4:+48389573 MsG0480020791.01.T01:CDS
GGCATGCCTGGTACTATTGG+TGG 0.484167 4:+48389576 MsG0480020791.01.T01:CDS
ACTGCGAAACACGCAGAGAA+AGG 0.497789 4:-48389599 None:intergenic
ACAAAATGTAAATGTTCCTA+TGG 0.529828 4:+48389533 MsG0480020791.01.T01:CDS
TGTTCCTATGGTGAGGATGA+AGG 0.538780 4:+48389545 MsG0480020791.01.T01:CDS
GCAACACCACCATCAGTAGT+TGG 0.541853 4:-48389501 None:intergenic
AAGGATATTCAAGGCATGCC+TGG 0.544386 4:+48389564 MsG0480020791.01.T01:CDS
GTGAGGATGAAGGATATTCA+AGG 0.554642 4:+48389555 MsG0480020791.01.T01:CDS
GAAGAAGTTCCAACTACTGA+TGG 0.579056 4:+48389492 MsG0480020791.01.T01:CDS
TACCAGAAGGCAGTGACAGA+AGG 0.584308 4:-48389672 None:intergenic
GAAAGGCCACCAATAGTACC+AGG 0.589357 4:-48389582 None:intergenic
ATATCCTTCATCCTCACCAT+AGG 0.600777 4:-48389549 None:intergenic
AGTGACAGAAGGAAAATCAC+TGG 0.602419 4:-48389661 None:intergenic
CAACTGGGTGAATTGAAGCA+TGG 0.610931 4:-48389470 None:intergenic
GACAGAAGGAAAATCACTGG+TGG 0.621848 4:-48389658 None:intergenic
AAACAAAACGGATTACCAGA+AGG 0.648151 4:-48389685 None:intergenic
TAGTTGGAACTTCTTCAACT+GGG 0.665313 4:-48389485 None:intergenic
ATGTAAATGTTCCTATGGTG+AGG 0.675797 4:+48389538 MsG0480020791.01.T01:CDS
GAAGTTCCAACTACTGATGG+TGG 0.707650 4:+48389495 MsG0480020791.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACAAAATGTAAATGTTCCTA+TGG + Chr4:48389533-48389552 MsG0480020791.01.T01:CDS 25.0%
ATGTAAATGTTCCTATGGTG+AGG + Chr4:48389538-48389557 MsG0480020791.01.T01:CDS 35.0%
TAGTTGGAACTTCTTCAACT+GGG - Chr4:48389488-48389507 None:intergenic 35.0%
!!! TGTTTCGCAGTTTGTTTTTG+CGG + Chr4:48389611-48389630 MsG0480020791.01.T01:CDS 35.0%
AGTGACAGAAGGAAAATCAC+TGG - Chr4:48389664-48389683 None:intergenic 40.0%
ATATCCTTCATCCTCACCAT+AGG - Chr4:48389552-48389571 None:intergenic 40.0%
GAAGAAGTTCCAACTACTGA+TGG + Chr4:48389492-48389511 MsG0480020791.01.T01:CDS 40.0%
GTAGTTGGAACTTCTTCAAC+TGG - Chr4:48389489-48389508 None:intergenic 40.0%
GTGAGGATGAAGGATATTCA+AGG + Chr4:48389555-48389574 MsG0480020791.01.T01:CDS 40.0%
AAGGATATTCAAGGCATGCC+TGG + Chr4:48389564-48389583 MsG0480020791.01.T01:CDS 45.0%
CAACTGGGTGAATTGAAGCA+TGG - Chr4:48389473-48389492 None:intergenic 45.0%
GAAGTTCCAACTACTGATGG+TGG + Chr4:48389495-48389514 MsG0480020791.01.T01:CDS 45.0%
GACAGAAGGAAAATCACTGG+TGG - Chr4:48389661-48389680 None:intergenic 45.0%
TGTTCCTATGGTGAGGATGA+AGG + Chr4:48389545-48389564 MsG0480020791.01.T01:CDS 45.0%
ACTGCGAAACACGCAGAGAA+AGG - Chr4:48389602-48389621 None:intergenic 50.0%
CAAGGCATGCCTGGTACTAT+TGG + Chr4:48389573-48389592 MsG0480020791.01.T01:CDS 50.0%
GAAAGGCCACCAATAGTACC+AGG - Chr4:48389585-48389604 None:intergenic 50.0%
GCAACACCACCATCAGTAGT+TGG - Chr4:48389504-48389523 None:intergenic 50.0%
GGCTGCTGCACTATCTGTTA+TGG + Chr4:48389632-48389651 MsG0480020791.01.T01:CDS 50.0%
TACCAGAAGGCAGTGACAGA+AGG - Chr4:48389675-48389694 None:intergenic 50.0%
TTCCTTCTGTCACTGCCTTC+TGG + Chr4:48389670-48389689 MsG0480020791.01.T01:CDS 50.0%
GGCATGCCTGGTACTATTGG+TGG + Chr4:48389576-48389595 MsG0480020791.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 48389459 48389695 48389459 ID=MsG0480020791.01;Name=MsG0480020791.01
Chr4 mRNA 48389459 48389695 48389459 ID=MsG0480020791.01.T01;Parent=MsG0480020791.01;Name=MsG0480020791.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|78
Chr4 exon 48389459 48389695 48389459 ID=MsG0480020791.01.T01:exon:17152;Parent=MsG0480020791.01.T01
Chr4 CDS 48389459 48389695 48389459 ID=MsG0480020791.01.T01:cds;Parent=MsG0480020791.01.T01
Gene Sequence

>MsG0480020791.01.T01

ATGAACGTTAGCCATGCTTCAATTCACCCAGTTGAAGAAGTTCCAACTACTGATGGTGGTGTTGCTGTTGCTGAACAAAATGTAAATGTTCCTATGGTGAGGATGAAGGATATTCAAGGCATGCCTGGTACTATTGGTGGCCTTTCTCTGCGTGTTTCGCAGTTTGTTTTTGCGGCTGCTGCACTATCTGTTATGGCTTCCACCAGTGATTTTCCTTCTGTCACTGCCTTCTGGTAA

Protein sequence

>MsG0480020791.01.T01

MNVSHASIHPVEEVPTTDGGVAVAEQNVNVPMVRMKDIQGMPGTIGGLSLRVSQFVFAAAALSVMASTSDFPSVTAFW*