AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014908.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014908.01.T01 MTR_3g031370 92.763 152 10 1 1 152 1 151 1.40e-84 245
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014908.01.T01 AT1G07020 50.000 148 66 4 9 150 1 146 3.18e-31 109

Find 2 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTCGATTGAATCAATAA+TGG 0.424877 3:+62304861 None:intergenic
GCCATTATTGATTCAATCGA+AGG 0.497435 3:-62304862 MsG0380014908.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAATTTTTGTTCTTGAT+CGG - Chr3:62304320-62304339 MsG0380014908.01.T01:exon 15.0%
!!! TGTTAACAAAATTGTGTTTT+GGG - Chr3:62304860-62304879 MsG0380014908.01.T01:intron 20.0%
!!! TTGTTAACAAAATTGTGTTT+TGG - Chr3:62304859-62304878 MsG0380014908.01.T01:intron 20.0%
!!! TTTGTTCTTCATTTTGATTA+TGG - Chr3:62304803-62304822 MsG0380014908.01.T01:intron 20.0%
AATTTGATGGAAATCATTCG+CGG + Chr3:62304451-62304470 None:intergenic 30.0%
ACCTTCGATTGAATCAATAA+TGG + Chr3:62304237-62304256 None:intergenic 30.0%
TCTTCAAACACTTGAAGATT+TGG - Chr3:62304759-62304778 MsG0380014908.01.T01:intron 30.0%
GATTTCCATCAAATTCCGAA+CGG - Chr3:62304456-62304475 MsG0380014908.01.T01:CDS 35.0%
TGTTCTTGATCGGTAGAAAA+CGG - Chr3:62304330-62304349 MsG0380014908.01.T01:exon 35.0%
!! GCCATTATTGATTCAATCGA+AGG - Chr3:62304233-62304252 MsG0380014908.01.T01:exon 35.0%
GTAGTATTCCGTAGCATCAT+CGG + Chr3:62304382-62304401 None:intergenic 40.0%
TGATCGGTAGAAAACGGAAA+CGG - Chr3:62304336-62304355 MsG0380014908.01.T01:exon 40.0%
TGTAACCGTTCGGAATTTGA+TGG + Chr3:62304464-62304483 None:intergenic 40.0%
TGTGAATTCCGATGATGCTA+CGG - Chr3:62304371-62304390 MsG0380014908.01.T01:CDS 40.0%
!! ATGAGATTCAGAGAGCGTTG+AGG - Chr3:62304580-62304599 MsG0380014908.01.T01:CDS 45.0%
!! TGAGATTCAGAGAGCGTTGA+GGG - Chr3:62304581-62304600 MsG0380014908.01.T01:CDS 45.0%
AAGGAGGATTGCACCGTTGA+CGG - Chr3:62304614-62304633 MsG0380014908.01.T01:CDS 50.0%
ATGCGGGGGATTTCGTTTGT+TGG - Chr3:62304666-62304685 MsG0380014908.01.T01:CDS 50.0%
CGTTGAGGGAAGAGGAAAGA+AGG - Chr3:62304595-62304614 MsG0380014908.01.T01:CDS 50.0%
GGAACTTCCGAAACCCATCT+CGG + Chr3:62304699-62304718 None:intergenic 50.0%
TGAGGGAAGAGGAAAGAAGG+AGG - Chr3:62304598-62304617 MsG0380014908.01.T01:CDS 50.0%
TGATCAATCCAACGCTCCTC+CGG + Chr3:62304720-62304739 None:intergenic 50.0%
TGTTGGAGAAGCTCCGAGAT+GGG - Chr3:62304683-62304702 MsG0380014908.01.T01:CDS 50.0%
TTGTTGGAGAAGCTCCGAGA+TGG - Chr3:62304682-62304701 MsG0380014908.01.T01:CDS 50.0%
! AGAAGCTCCGAGATGGGTTT+CGG - Chr3:62304689-62304708 MsG0380014908.01.T01:CDS 50.0%
! GAGATGGGTTTCGGAAGTTC+CGG - Chr3:62304698-62304717 MsG0380014908.01.T01:CDS 50.0%
ATGGGTTTCGGAAGTTCCGG+AGG - Chr3:62304701-62304720 MsG0380014908.01.T01:CDS 55.0%
GAGGAAGAGGAGGAGAGAAG+CGG - Chr3:62304543-62304562 MsG0380014908.01.T01:CDS 55.0%
GATGCTGGATCTGAACGACG+AGG - Chr3:62304515-62304534 MsG0380014908.01.T01:CDS 55.0%
GGAGAATGCGACAAGGGTGA+TGG - Chr3:62304638-62304657 MsG0380014908.01.T01:CDS 55.0%
!! AAGGGTGATGGAAGCGATGC+GGG - Chr3:62304650-62304669 MsG0380014908.01.T01:CDS 55.0%
!! CAAGGGTGATGGAAGCGATG+CGG - Chr3:62304649-62304668 MsG0380014908.01.T01:CDS 55.0%
!! TCAGAGAGCGTTGAGGGAAG+AGG - Chr3:62304587-62304606 MsG0380014908.01.T01:CDS 55.0%
AACGGTTACACTGCGCACGG+TGG - Chr3:62304474-62304493 MsG0380014908.01.T01:CDS 60.0%
ATATCCGCCGTCGACGACGA+CGG - Chr3:62304408-62304427 MsG0380014908.01.T01:CDS 60.0%
CCGAACGGTTACACTGCGCA+CGG - Chr3:62304471-62304490 MsG0380014908.01.T01:CDS 60.0%
CCGTGCGCAGTGTAACCGTT+CGG + Chr3:62304474-62304493 None:intergenic 60.0%
CGTCGACGGCGGATATTGGT+TGG + Chr3:62304404-62304423 None:intergenic 60.0%
GACGACGACGGCAGTTCAGA+CGG - Chr3:62304420-62304439 MsG0380014908.01.T01:CDS 60.0%
GACGGCGGAGAATGCGACAA+GGG - Chr3:62304632-62304651 MsG0380014908.01.T01:CDS 60.0%
GAGGATTGCACCGTTGACGG+CGG - Chr3:62304617-62304636 MsG0380014908.01.T01:CDS 60.0%
GCTGGATCTGAACGACGAGG+AGG - Chr3:62304518-62304537 MsG0380014908.01.T01:CDS 60.0%
GGATCTGAACGACGAGGAGG+AGG - Chr3:62304521-62304540 MsG0380014908.01.T01:CDS 60.0%
GGTGAACGGGATCTCGATGC+TGG - Chr3:62304500-62304519 MsG0380014908.01.T01:CDS 60.0%
GTCGCATTCTCCGCCGTCAA+CGG + Chr3:62304630-62304649 None:intergenic 60.0%
TCGTCGTCGACGGCGGATAT+TGG + Chr3:62304408-62304427 None:intergenic 60.0%
TCTGAACGACGAGGAGGAGG+AGG - Chr3:62304524-62304543 MsG0380014908.01.T01:CDS 60.0%
TGAACTGCCGTCGTCGTCGA+CGG + Chr3:62304418-62304437 None:intergenic 60.0%
TGACGGCGGAGAATGCGACA+AGG - Chr3:62304631-62304650 MsG0380014908.01.T01:CDS 60.0%
TTACACTGCGCACGGTGGAG+CGG - Chr3:62304479-62304498 MsG0380014908.01.T01:CDS 60.0%
!! AGGGTGATGGAAGCGATGCG+GGG - Chr3:62304651-62304670 MsG0380014908.01.T01:CDS 60.0%
CGACGAGGAGGAGGAGGAAG+AGG - Chr3:62304530-62304549 MsG0380014908.01.T01:CDS 65.0%
CGAGGAGGAGGAGGAAGAGG+AGG - Chr3:62304533-62304552 MsG0380014908.01.T01:CDS 65.0%
! CGGAAGTTCCGGAGGAGCGT+TGG - Chr3:62304709-62304728 MsG0380014908.01.T01:CDS 65.0%
!! GGGTGATGGAAGCGATGCGG+GGG - Chr3:62304652-62304671 MsG0380014908.01.T01:CDS 65.0%
ACTGCCGTCGTCGTCGACGG+CGG + Chr3:62304415-62304434 None:intergenic 70.0%
CGCACGGTGGAGCGGTGAAC+GGG - Chr3:62304487-62304506 MsG0380014908.01.T01:CDS 70.0%
GCGCACGGTGGAGCGGTGAA+CGG - Chr3:62304486-62304505 MsG0380014908.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 62304206 62304911 62304206 ID=MsG0380014908.01;
Chr3 mRNA 62304206 62304911 62304206 ID=MsG0380014908.01.T01;Parent=MsG0380014908.01
Chr3 exon 62304206 62304771 62304206 ID=MsG0380014908.01.T01.exon;Parent=MsG0380014908.01.T01
Chr3 CDS 62304344 62304771 62304344 ID=MsG0380014908.01.T01.cds;Parent=MsG0380014908.01.T01
Chr3 CDS 62304863 62304893 62304863 ID=MsG0380014908.01.T01.cds;Parent=MsG0380014908.01.T01
Chr3 exon 62304863 62304911 62304863 ID=MsG0380014908.01.T01.exon;Parent=MsG0380014908.01.T01
Gene Sequence

>MsG0380014908.01.T01

ATGAACAGTGCCATTATTGATTCAATCGAAGAAAACGGAAACGGAAGCGATTCAGATGTGAATTCCGATGATGCTACGGAATACTACCAACCAATATCCGCCGTCGACGACGACGGCAGTTCAGACGGAGAAACCGCGAATGATTTCCATCAAATTCCGAACGGTTACACTGCGCACGGTGGAGCGGTGAACGGGATCTCGATGCTGGATCTGAACGACGAGGAGGAGGAGGAAGAGGAGGAGAGAAGCGGAGAAGAAGTTGAAAATGAGATTCAGAGAGCGTTGAGGGAAGAGGAAAGAAGGAGGATTGCACCGTTGACGGCGGAGAATGCGACAAGGGTGATGGAAGCGATGCGGGGGATTTCGTTTGTTGGAGAAGCTCCGAGATGGGTTTCGGAAGTTCCGGAGGAGCGTTGGATTGATCAGATTCGAAGACGAAGACAATCTTCAAACACTTGA

Protein sequence

>MsG0380014908.01.T01

MNSAIIDSIEENGNGSDSDVNSDDATEYYQPISAVDDDGSSDGETANDFHQIPNGYTAHGGAVNGISMLDLNDEEEEEEEERSGEEVENEIQRALREEERRRIAPLTAENATRVMEAMRGISFVGEAPRWVSEVPEERWIDQIRRRRQSSNT*